06-07-2017 дата публикации
Номер: US20170189558A1
The present disclosure relates to the production and use of ultrasmall luminescent nanoparticles coupled to dyes and the obtained nanoparticles exhibit functionalities that can be highly sensitive to the local chemical environment changes. These nanoparticles may act as ultrasmall nanosensors to ratiometrically report pH or glutathione in the local environment. 1. A composition comprising a noble metal nanoparticle , wherein the surface of the nanoparticle is conjugated with (a) a charged ligand susceptible to protonation or deprotonation by pH change and (b) a dye that is pH-insensitive when not conjugated with the noble metal nanoparticle.2. The composition of claim 1 , wherein the noble metal is gold.3. The composition of claim 1 , wherein the nanoparticle is about 0.5 nm to 10 nm in diameter claim 1 , or 1 nm to 5 nm in diameter.4. The composition of claim 1 , wherein the nanoparticle is luminescent.56.-. (canceled)7. The composition of claim 1 , wherein the charged ligand is glutathione claim 1 , cysteine claim 1 , cysteine-glycine claim 1 , cysteine-glutamate claim 1 , or any other ligand that is differentially protonated across various pHs.813.-. (canceled)14. The composition of claim 1 , wherein the composition comprises two or more pH insensitive dye molecules.1516.-. (canceled)17. The composition of claim 1 , wherein the nanoparticle is sensitive to a pH from about 5 to about 11 claim 1 , from about 6 to about 10 claim 1 , from about 7 to about 9 claim 1 , or at about pH 5 claim 1 , 6 claim 1 , 7 claim 1 , 8 claim 1 , 9 claim 1 , 10 or 11.18. The composition of claim 1 , noble metal may consist of claim 1 , comprise claim 1 , or consist essentially of silver claim 1 , copper claim 1 , platinum claim 1 , or carbon claim 1 , and optionally is luminescent.19. The composition of claim 18 , wherein the size of the nanoparticle is less than 3 nm.20. A method for detecting pH of an environment comprising the steps of:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1 ...
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