05-05-2016 дата публикации
Номер: US20160121293A1
Принадлежит:
Provided are a labelling agent including a compound of the following Formula 1, a labelling composition, a method of labelling a protein with the labelling agent, and a method of detecting the protein using the labelling agent: 2. The labelling agent of claim 1 , wherein R claim 1 , R claim 1 , R claim 1 , R claim 1 , R claim 1 , R claim 1 , and Rare independently H claim 1 , substituted or unsubstituted —C-alkyl- claim 1 ,{'sub': 7', '9', '10', '9', '10', '1-10, 'Ris H or —NRR, Rand Rare independently H, substituted or unsubstituted —C-alkyl-,'}{'sub': 11', '1-10, 'Ris H, substituted or unsubstituted —C-alkyl-,'}{'sub': 12', '1-10, 'Ris H, substituted or unsubstituted —C-alkyl-, and'}{'sub': 13', '14', '1-10, 'Rand Ris independently H, substituted or unsubstituted —C-alkyl-.'}6. The composition of claim 4 , wherein the compound is covalently linked to the protein.8. The method of claim 7 , wherein the irradiating light causes a radical reaction of the labelling agent to form a complex of the labelling agent and the protein. This application claims the benefit of Korean Patent Application No. 10-2014-0150621, filed on Oct. 31, 2014, in the Korean Intellectual Property Office, the disclosure of which is incorporated herein in its entirety by reference.1. FieldThe present disclosure relates to a labelling agent, a composition for labelling a protein, a method of labelling a protein using the labelling agent, and a method of detecting a protein.2. Description of the Related ArtA technology of labelling in vivo proteins is one of fields which are of great importance in molecular biology, since development of green fluorescence protein (GFP). This technology is used to monitor protein migration according to cell proliferation and signal transduction as well as location of proteins present in an organism, and thus has a great function to overcome resolution of the existing microscopes and to find out a new mechanism.To label proteins with GFP, transfection using virus is ...
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