Lead pollution detection probe and lead ion detection method

The invention belongs to the technical field of biological detection, and particularly relates to a lead pollution detection probe and a lead ion detection method. The kit is composed of a probe EDP and a probe RFP, wherein the probe EDP comprises a substrate chain combined with an RNA base (rA) and an enzyme chain; the probe RFP is a hairpin probe, the stem part of the hairpin probe RFP is provided with a fluorophore FAM and a quenching group Dabcyl, the ring part of the hairpin probe RFP is provided with a recognition site for cutting and carving an incision enzyme, and the method combines DNAzyme with an enzyme-assisted amplification strategy to realize low-cost, simple, convenient and efficient detection of Pb < 2 + >.