Herstellung von Pflanzen mit verbesserter Wurzelbildung und verbesserter Vasen-Lebensdauer durch Verwendung von Stressresistenzgenen

TOWARDS FOR THE FATTY ACID DESATURASE ONES, MEANING GENE CONTAINING VECTOR, A PLANT, INTO WHICH MEANT GENE IS BROUGHT, AND A PROCEDURE FOR THE CREATION OF MEANING PLANT

Gene for fatty acid desaturase, vector containing said gene, plant containing said gene transferred thereinto, and process for creating said plant

IMPROVED PROMOTERS AND THEIR USE

Dna strands encoding glycerol-3-phosphate acyltransferase

Beta-ketoacyl-acp-synthetase ii enzyme and gene encoding the same

DNA STRANDS CODING FOR GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE

DNA strands having the ability to biotechnologically produce glycerol-3-phosphate acyltransferase (ATase) useful for converting the property of the PG of membrane lipids into that of more chilling resistance, specifically a chimeric gene of glycerol-3-phosphate acyltransferase (ATase) cDNA derived from squash in which the about one- third central region (the site cleaved by Kpn I and Hind III) has been replaced with the corresponding region of spinach ATase cDNA, a cDNA derived from squash in which the about one-sixth central region (the site cleaved by Hind III and Sac I) has been replaced with the corresponding region of spinach ATase cDNA, or a chimeric gene of ATase cDNA, derived from spinach in which the about one-third 3'- terminal region (the site cleaved by Kpn I and Eco RI) has been replaced with the corresponding region of squash ATase cDNA are disclosed. These chimeric genes can express a chimeric ATase which has a higher substrate selectivity to unsaturated fatty acids. The ...

.BETA.-KETOACYL-ACP-SYNTHETASE II ENZYMES AND GENES CODING FOR SAME

A gene of a protein having an enzymatic activity enabling the control or regulation of the contents of saturated and unsaturated fatty acids in plant cells and the enzyme protein as the expression product of this gene, more specifically: proteins showing a .beta.-ketoacyl-ACP-synthetase II (KAS II) activity and having a specific amino acid sequence typified by the amino acid sequence of KAS II originating in blue-green algae (Anacystis nidulans) or an amino acid sequence substantially equivalent thereto; a KAS II protein gene encoding the amino acid sequence of the above protein; and recombinant vectors containing this gene and cells having this gene transferred thereinto.

.BETA.-KETOACYL-ACP SYNTHETASE II ENZYMES AND GENES CODING FOR SAME

The object of the present invention is to provide a gene of a protein having an enzyme activity which makes it possible to regulate or control the content of saturated fatty acids and unsaturated fatty acids in plant cells, and an enzyme protein as the expression product. There is disclosed a protein with a KASII enzyme activity which has a specific amino acid sequence, typically the amino acid sequence of a .beta.-ketoacyl-ACP synthetase II (KASII) enzyme protein derived from cyanobacterium (Anacystis nidulans) or substantially the same amino acid sequence as the one described above. There is also disclosed a KASII enzyme protein gene coding for the amino acid sequence of the above described protein. Furthermore, there are disclosed a recombinant vector which comprises said gene, and a cell into which said gene has been introduced.

Promoters and utilization thereof

The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which as been considered a high expression promoter for plants.

Gene for Fatty Acid Desaturase, Vector Containing Said Gene, Plant Transformed with Said Gene, and Process for Creating Said Plant

GENE FOR FATTY ACID DESATURASE, VECTOR CONTAINING SAID GENE, PLANT TRANSFORMED WITH SAID GENE, AND PROCESS FOR CREATING SAID PLANT

Genes encoding proteins having an activity of desaturating lipid-bound fatty acids at the .DELTA.9 position; vectors which contain the genes or polynucleotides containing part of thereof; plant cells transformed with the genes or polynucleotides containing part of thereof; a method for creating plants by regenerating the plant cells to reproduce mature plants; and plants transformed with the genes or polynucleotides containing part of thereof.

DNA STRANDS CODING FOR GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE

Techniques relating to DNA strands having a bioengineering productivity of glycerol-3-phosphate acyltransferase (ATase) which is useful in the conversion of a membrane lipid PG into one having improved cold resistance, more particularly, a chimeric gene wherein the central region (about 1/3; cleaved with Kpn I and Hind III) of glycerol-3-phosphate ATase cDNA originating in pumpkin has been replaced by the corresponding region of spinach ATase cDNA; a chimeric gene wherein the central region (about 1/6; cleaved with Hind III Sac I) of cDNA originating in pumpkin has been replaced by cDNA originating in spinach; or a chimeric gene wherein the 3'-terminal region (about 1/3; cleaved with Kpn I and Eco RI) of cDNA of ATase originating in spinach has been replaced by the corresponding region of cDNA of pumpkin ATase. Such chimeric genes can express a chimeric ATase having an enhanced substrate-specificity for unsaturated fatty acids. When this DNA strand is transduced into a lowtemperature-sensitive ...

Gene for fatty acid desaturase, vector containing said gene, plant containing said gene transferred thereinto, and process for creating said plant

DNA strands encoding glycerol-3-phosphate acyltransferase

Production of plants having improved rooting efficiency and vase life using stress-resistance gene

Provided is a plant having improved efficiency in propagation by cutting resulting from the enhanced rooting efficiency, and improved vase life. A method of producing a transformed plant having improved rooting efficiency and/or prolonged vase life, comprising transforming a plant using a gene wherein a DNA encoding a protein that binds to a stress-responsive element contained in a stress-responsive promoter and regulates the transcription of a gene located downstream of the element is ligated downstream of the stress-responsive promoter.

GEN FÜR DIE FETTSÄURE-DESATURASE, BESAGTES GEN ENTHALTENDER VEKTOR, EINE PFLANZE, IN DIE BESAGTES GEN EINGEBRACHT IST, UND EIN VERFAHREN ZUR SCHAFFUNG BESAGTER PFLANZE

Dna chain coding for glycero-3-phosphate acyltransferase and use thereof

GEN FÜR DIE FETTSÄURE-DESATURASE, BESAGTES GEN ENTHALTENDER VEKTOR, EINE PFLANZE, IN DIE BESAGTES GEN EINGEBRACHT IST, UND EIN VERFAHREN ZUR SCHAFFUNG BESAGTER PFLANZE

IMPROVED PROMOTERS AND UTILIZATION THEREOF

Promoters having been improved so as not to undergo methylation in the course of constructing transformants. The above promoters are DNAs as defined by the following (1) or (2): (1) DNAs containing the base sequences represented by SEQ ID NOS:1 to 4; and (2) DNAs containing base sequences derived from the base sequences represented by SEQ ID NOS:1 to 4 by deletion, addition or insertion of one to several bases, being free from the consecutive sequences represented by CG, CAG, CTG, CCG and CGG in the deleted, added or inserted base sequence, and having a promoter activity. By using these promoters, the expression efficiency of a structural gene of a plant showing weak expression even by using cauliflower mosaic virus 35S promoter, which has been considered as a high-expression promoter for plants, (for example, chrysanthemum) can be elevated. © KIPO & WIPO 2007 ...

VERBESSERTE PROMOTOREN UND DEREN VERWENDUNG

BETA-KETOACYL-ACP-SYNTHELASE II-ENZYM UND DAFÜR KODIERENDES GEN

Peptide transporting to chromoplasts in petals and method of constructing plant having yellowish petals by using the same

It is an object of the present invention to provide a means of imparting a yellowish flower color to a plant having no or little yellowish flower color or enhancing the yellowish flower color in a plant. In accordance with the present invention, a method of producing a plant having yellowish petals with the use of a peptide having transport activity to chromoplasts in petals and a fused gene formed of a gene encoding such peptide and one, or two or more genes encoding enzyme proteins involved in the carotenoid biosynthetic pathway is provided.

Beta-ketoacyl-acp-synthetase II enzyme and gene encoding same

Gene for fatty acid desaturase, vector containing said gene, plant cell containing said gene transferred thereinto

beta-ketoacyl-ACP synthetase II enzymes and genes coding for same

The object of the present invention is to provide a gene of a protein having an enzyme activity which makes it possible to regulate or control the content of saturated fatty acids and unsaturated fatty acids in plant cells, and an enzyme protein as the expression product.There is disclosed a protein with a KASII enzyme activity which has a specific amino acid sequence, typically the amino acid sequence of a beta-ketoacyl-ACP synthetase II (KASII) enzyme protein derived from cyanobacterium (Anacystis nidulans) or substantially the same amino acid sequence as the one described above.There is also disclosed a KASII enzyme protein gene coding for the amino acid sequence of the above described protein.Furthermore, there are disclosed a recombinant vector which comprises said gene, and a cell into which said gene has been introduced.

Peptide Transporting To Chromoplasts In Petals And Method Of Constructing Plant Having Yellowish Petals By Using The Same

It is an object of the present invention to provide a means of imparting a yellowish flower color to a plant having no or little yellowish flower color or enhancing the yellowish flower color in a plant. In accordance with the present invention, a method of producing a plant having yellowish petals with the use of a peptide having transport activity to chromoplasts in petals and a fused gene formed of a gene encoding such peptide and one, or two or more genes encoding enzyme proteins involved in the carotenoid biosynthetic pathway is provided.

DNA strands encoding glycerol-3-phosphate acyltransferase

Promoters and utilization thereof

The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which as been considered a high expression promoter for plants.

Gene for fatty acid desaturase, vector containing said gene, plant containing said gene transferred thereinto, and process for creating said plant

Beta-ketoacyl-ACP-synthetase Ôàí enzyme and gene encoding same

Peptide migrating into chromoplast in petal and method of constructing plant having yellowish petal by using the peptide

It is an object of the present invention to provide a means of imparting a yellowish flower color to a plant having no or little yellowish flower color or enhancing the yellowish flower color in a plant. In accordance with the present invention, a method of producing a plant having yellowish petals with the use of a peptide having transport activity to chromoplasts in petals and a fused gene formed of a gene encoding such peptide and one, or two or more genes encoding enzyme proteins involved in the carotenoid biosynthetic pathway is provided.

Beta-ketoacyl-acp-synthetase ii enzyme and gene encoding the same

GEN THAT CODIFIES FAT-DESATURASE ACID, VECTOR CONTAINING SUCH GEN, PLANT CONTAINING SUCH GEN TRANSFERRED TO HER AND PROCEDURE TO CREATE SUCH PLANT.

UN GEN QUE CODIFICA UNA PROTEINA QUE TIENE UNA ACTIVIDAD DE DESATURACION DE LA POSICION {DE}9 DE UN ENLACE DE ACIDO GRASO A UN LIPIDO; UN VECTOR QUE CONTIENE UN POLINUCLEOTIDO QUE CONTIENE LA TOTALIDAD O PARTE DE DICHO GEN; UNA CELULA DE PLANTA QUE CONTIENE, TRANSFERIDO A LA MISMA, UN POLINUCLEOTIDO QUE CONTIENE LA TOTALIDAD O PARTE DE UN GEN QUE CODIFICA UNA PROTEINA QUE TIENE LA ACTIVIDAD DE DESATURACION DE LA POSICION {DE}9 DE UN ENLACE DE ACIDO GRASO A UN LIPIDO; UN PROCESO PARA CREAR UNA PLANTA QUE INCLUYE LA DIFERENCIACION DE DICHAS CELULAS DE PLANTA Y LA REGENERACION DEL CUERPO DE LA PLANTA; UNA PLANTA QUE CONTIENE, TRANSFERIDO EN LA MISMA, UN POLINUCLEOTIDO QUE CONTIENE LA TOTALIDAD O PARTE DE UN GEN QUE CODIFICA UNA PROTEINA QUE POSEE LA ACTIVIDAD DE DESATURACION DE LA POSICION {DE}9 DE UN ENLACE DE ACIDO GRASO A UN LIPIDO. A GENE THAT CODIFIES A PROTEIN THAT HAS A POSITION DESATURATION ACTIVITY {DE} 9 OF A GREEN ACID LINK TO A LIPID; A VECTOR CONTAINING A POLINUCLEOTIDE CONTAINING THE TOTALITY OR PART OF THIS GEN; A PLANT CELL THAT CONTAINS, TRANSFERRED TO IT, A POLINUCLEOTIDE CONTAINING THE TOTALITY OR PART OF A GENE THAT CODIFIES A PROTEIN THAT HAS THE ACTIVITY OF DESATURATION OF THE POSITION {OF} 9 OF A GREEN ACID LINK TO A LIPID; A PROCESS TO CREATE A PLANT THAT INCLUDES THE DIFFERENTIATION OF SUCH PLANT CELLS AND THE REGENERATION OF THE PLANT BODY; A PLANT THAT CONTAINS, TRANSFERRED IN THE SAME, A POLINUCLEOTIDE THAT CONTAINS THE TOTALITY OR PART OF A GENE THAT CODIFIES A PROTEIN THAT POSSESSES THE ACTIVITY OF DESATURATION OF THE POSITION {OF} 9 OF A GREEN ACID LINK TO A LIPID.

Peptide migrating into chromoplast in petal and method of constructing plant having yellowish petal by using the peptide

It is intended to provide a means of imparting a yellowish flower color to a plant, which has no or little yellowish color, or strengthening such a color. Namely, a peptide having an activity of migrating into chromoplast in petal; and a method of constructing a plant having yellowish petal by using a fused gene composed of a gene encoding the above peptide with one or more genes encoding enzyme proteins participating in the carotenoid synthesis system.

ENZYM BETA-CETOACIL-ACP-SINTETASA II AND GEN THAT CODIFIES IT.

Una proteína aislada de cianobacterias seleccionadas del grupo formado por: a) el cual tiene la secuencia de aminoácidos representada por SEQ ID NO: 2; b) el cual tiene la secuencia de aminoácidos representada por SEQ ID NO: 2 en donde un aminoácido está substituido, suprimido, insertado o añadido, y tiene la actividad de la enzima Beta-cetoacil-ACP sintetasa II; c) el cual es codificado por un nucleótido que comprende un mutante degenerado de la secuencia representada por SEQ ID NO: 1 y codifica una proteína de SEQ ID NO: 2; d) el cual es codificado por un nucleótido que comprende una secuencia representada por SEQ ID NO: 1, en donde dicho nucleótido codifica un polipéptido que tiene la actividad de la enzima Beta-cetoacil-ACP sintetasa II; e) el cual es codificado por un nucleótido capaz de hibridar con la secuencia de c) ó d) en una solución de hibridación que comprende 10xDenhardt, 5 x SSC, 10 mM de EDTA, 250 mug/ml de ADN de esperma de salmón a 60ºC durante 16 horas, seguido por un lavado con una solución con 5xSSC, 0,1% de SDS a 45ºC durante 15 minutos, en donde dicho nucleótido tiene una secuencia que codifica un polipéptido que tiene la actividad de la enzima Beta-cetoacil- ACP sintetasa II. An isolated protein from cyanobacteria selected from the group consisting of: a) which has the amino acid sequence represented by SEQ ID NO: 2; b) which has the amino acid sequence represented by SEQ ID NO: 2 wherein an amino acid is substituted, deleted, inserted or added, and has the activity of the enzyme Beta-ketoacyl-ACP synthetase II; c) which is encoded by a nucleotide comprising a degenerate mutant of the sequence represented by SEQ ID NO: 1 and encodes a protein of SEQ ID NO: 2; d) which is encoded by a nucleotide comprising a sequence represented by SEQ ID NO: 1, wherein said nucleotide encodes a polypeptide having the activity of the enzyme Beta-ketoacyl-ACP synthetase II; e) which is encoded by a nucleotide capable of hybridizing with the sequence of c) or d) ...

Production of plants having improved rooting efficiency and vase life using stress-resistance gene

Provided is a plant having improved efficiency in propagation by cutting resulting from the enhanced rooting efficiency, and improved vase life. A method of producing a transformed plant having improved rooting efficiency and/or prolonged vase life, comprising transforming a plant using a gene wherein a DNA encoding a protein that binds to a stress-responsive element contained in a stress-responsive promoter and regulates the transcription of a gene located downstream of the element is ligated downstream of the stress-responsive promoter.

Production of plants having improved rooting efficiency and vase life using stress-resistance gene

Provided is a plant having improved efficiency in propagation by cutting resulting from the enhanced rooting efficiency, and improved vase life. A method of producing a transformed plant having improved rooting efficiency and/or prolonged vase life, comprising transforming a plant using a gene wherein a DNA encoding a protein that binds to a stress-responsive element contained in a stress-responsive promoter and regulates the transcription of a gene located downstream of the element is ligated downstream of the stress-responsive promoter.

Gen für die fettsäure-desaturase, besagtes gen enthaltender vektor, eine pflanze, in die besagtes gen eingebracht ist, und ein verfahren zur schaffung besagter pflanze

Verbesserte promotoren und deren verwendung