18-02-2021 дата публикации
Номер: US20210046479A1
Принадлежит:
Disclosed herein is an integrated microfluidic chip for detecting cancerous cells, particularly, cholangio-cancerous cells, from a biological sample. Also disclosed herein is a method of detecting cholangio-cancerous cells from a biological sample. 2. The method of claim 1 , wherein the sulfated octasaccharide of formula (I) is further coupled to a biotin.3. The method of claim 1 , wherein in the sulfated octasaccharide of formula (I) claim 1 , Ris —NHSONa; and Rand Rare independently —SONa.4. The method of claim 1 , wherein in the sulfated octasaccharide of formula (I) claim 1 , Ris —NHSONa claim 1 , Rand Rare independently H.5. The method of claim 1 , wherein the biological sample is selected from the group consisting of blood claim 1 , plasma claim 1 , serum claim 1 , urine claim 1 , sputum claim 1 , saliva claim 1 , tissue sample claim 1 , biopsy claim 1 , and tissue lysate.6. A microfluid chip comprising:a plurality of wash buffer chambers respectively configured to hold a wash buffer therein;a plurality of capture chambers respectively configured to capture a cancerous cell on a magnetic bead pre-coated with a biomarker of the cancerous cell;a waste chamber configured to hold uncaptured cancerous cell washed out from the plurality of capture chambers; anda plurality of microchannels connecting the wash buffer chamber, the plurality of capture chambers and the waste chamber.7. The microfluidic chip of claim 6 , wherein the microfluidic chip is fabricated on a glass substrate and at least one layer of polydimethylsiloxane.8. The microfluidic chip of claim 7 , wherein each of the capture chambers is configured to isolate the magnetic bead with bound cancerous cell from a fluid sample.10. The microfluidic chip of claim 9 , wherein the sulfated octasaccharide of formula (I) is further coupled to a biotin.11. The microfluidic chip of claim 9 , wherein in the sulfated octasaccharide of formula (I) claim 9 , Ris —NHSONa; and Rand Rare independently —SONa.12. The ...
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