20-06-2013 дата публикации
Номер: US20130157345A1
Methods for use of select organic compounds for the activation and stabilization of thermally labile enzymes, including proteases, are disclosed. The invention further relates to compositions of certain organic compounds with enzymes to enable the use of such enzymes at high temperature conditions, such as for warewash temperatures and conditions. In particular, organic activator-stabilizers including heat-deactivated proteases and zwitterionic materials are combined with enzymes according to the invention. As a result, the invention provides enzyme cleaning efficacy for various high temperature conditions, including warewash applications. 1. A method of cleaning a hard surface comprising:contacting the hard surface with an aqueous use solution composition comprising from about 0.1 ppm. to about 10,000 ppm enzymes, and from about 0.1 ppm to about 10,000 ppm organic activator-stabilizers, wherein said organic activator-stabilizers increase stabilization of said enzymes at temperatures in excess of at least 150° F. up to temperatures of 180° F.2. The method of claim 1 , wherein said enzyme is a protease and said organic activator-stabilizer is a heat deactivated enzyme.3. The method of claim 1 , where said organic activator-stabilizers are selected froth the group consisting of heat-deactivated enzymes claim 1 , denatured enzymes claim 1 , zwitterionic materials and combinations of the same claim 1 , and wherein said zwitterionic materials are members of the group consisting of amphoteric surfactants claim 1 , amino acids claim 1 , proteins and combinations of the same.4. The method of claim 1 , wherein said enzyme is selected from the group consisting of protease claim 1 , amylase claim 1 , lipase claim 1 , cellulase claim 1 , cutinase claim 1 , gluconase claim 1 , peroxidase and combinations of the same.5. The method of claim 2 , wherein said protease in said aqueous use solution is between about 1 ppm and about 400 ppm claim 2 , and wherein said heat deactivated ...
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