12-03-2020 дата публикации
Номер: US20200080046A1
Принадлежит:
Embodiments of the disclosed subject matter provide an automated method and system to isolate and collect cells using computerized analysis of images of cells and their surroundings obtained from a digital imaging device or system. Embodiments of the disclosed subject matter make use of a “microwell array,” which can comprise a formed, elastomeric grid of indentations or “wells.” Many, most, or all of the wells in a microwell array can contain a releasable, microfabricated element, which can be referred to as a “raft.” Embodiments of the disclosed subject matter provide a system and method for cell collection that includes computerized identification and collection of rafts with isolated single cells or a specific group or groups of cells, eliminating the need for continuous human identification and selection. 1. An automated , computer-controlled method for monitoring and collecting selected cells , the method comprising: an imaging device configured for obtaining the images of the microwell array;', 'an actuator configured for releasing the cell rafts from the microwell array;', 'a mechanical cell raft collector; and', obtaining the images of the microwell array using the imaging device;', 'identifying, by analyzing the images of the microwell array, the at least one selected cell raft that contains a cell undergoing a cellular process, storing a location of the selected cell raft on the microwell array, and assigning the selected cell raft to a mapped location of a collection plate;', 'controlling the actuator to release the selected cell raft from the microwell array; and', 'controlling the mechanical cell raft collector to collect the selected cell raft after release from the microwell array and controlling the mechanical cell raft collector to deposit the selected cell raft at the mapped location of the collection plate;, 'a computer system comprising at least one processor and memory, the computer system configured for], 'i) using a system, imaging a ...
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