INTRACELLULAR DELIVERY VEHICLE

Disclosed are an intracellular delivery vehicle the surface of which is covered by a positive charge, an intracellular delivery complex in which the intracellular delivery vehicle is filled with a component or compound of interest, a temperature-sensitive probe including the intracellular delivery complex, and a method for measuring the temperature inside a cell by the temperature-sensitive probe. The intracellular delivery vehicle is useful insofar as it makes it possible to deliver a desired component or compound easily to within a cell without disturbing cell proliferation.

INTRACELLULAR DELIVERY VEHICLE

INTRACELLULAR DELIVERY VEHICLE

An intracellular delivery vehicle of which surface is covered by a positive charge, an intracellular delivery complex in which a component or compound desired is loaded in the intracellular delivery vehicle, a temperature-sensitive probe comprising the intracellular delivery complex, and a method for measuring the intracellular temperature by the temperature-sensitive probe are disclosed. The intracellular delivery vehicle is useful on account of its capability of easily delivering the component or compound desired inside the cell without inhibiting cell proliferation.

TEMPERATURE-SENSITIVE FLUORESCENT PROBE FOR INTRODUCTION INTO CELL

Provided is a method for introducing into a cell a temperature-sensitive probe comprising a copolymer containing a heat-sensitive unit and a fluorescent unit. This method uses the above copolymer also containing a cationic unit as a temperature-sensitive probe and includes a step for mixing the copolymer with a cell in a solvent. Due to the presence of cationic groups, the copolymer can be used suitably as a fluorescent temperature sensor for measuring the temperature inside a cell because the copolymer can penetrate inside the cell without using any special method.

INTRACELLULAR DELIVERY VEHICLE

Temperature-sensitive fluorescent probe for introduction into cell

There is provided a method for introducing a temperature-sensitive probe comprising a copolymer, which comprises a thermoresponsive unit and a fluorescent unit, into a cell, and the method using the copolymer further comprising a cationic unit as the temperature-sensitive probe, and the method comprising the step of mixing the copolymer with the cell in a solvent. The copolymer can be preferably used as a fluorescence temperature sensor which measures intracellular temperature since the copolymer has a cationic group and thus enters into a cell without using a special method.

Temperature-sensitive fluorescent probe for introduction into cell

There is provided a method for introducing a temperature-sensitive probe comprising a copolymer, which comprises a thermoresponsive unit and a fluorescent unit, into a cell, and the method using the copolymer further comprising a cationic unit as the temperature-sensitive probe, and the method comprising the step of mixing the copolymer with the cell in a solvent. The copolymer can be preferably used as a fluorescence temperature sensor which measures intracellular temperature since the copolymer has a cationic group and thus enters into a cell without using a special method.

INTRACELLULAR DELIVERY VEHICLE

An intracellular delivery vehicle of which surface is covered by a positive charge, an intracellular delivery complex in which a component or compound desired is loaded in the intracellular delivery vehicle, a temperature-sensitive probe comprising the intracellular delivery complex, and a method for measuring the intracellular temperature by the temperature-sensitive probe are disclosed. The intracellular delivery vehicle is useful on account of its capability of easily delivering the component or compound desired inside the cell without inhibiting cell proliferation. 16-. (canceled)8. The compound according to claim 7 , wherein said Y and Z represent single bond.9. The compound according to claim 7 , wherein said R claim 7 , R claim 7 , R claim 7 , and Rare each independently selected from the group consisting of methyl group claim 7 , ethyl group claim 7 , methylcarbonyl group claim 7 , isobutyl group claim 7 , and 2-methyl-2-methoxypropyl group.10. The compound according to claim 7 , wherein said Rand Rare a methyl group.11. The compound according to claim 7 , wherein said Rand R claim 7 , said Rand R claim 7 , said Rand R claim 7 , said Rand R claim 7 , said Rand R claim 7 , and said Rand R claim 7 , each represent an identical substituent claim 7 , and said Y and Z represent an identical substituent or a single bond.12. The compound according to claim 7 , wherein said R claim 7 , R claim 7 , R claim 7 , R claim 7 , R claim 7 , R claim 7 , R claim 7 , and Rare a methyl group claim 7 , and said R claim 7 , R claim 7 , Rand Rare a hydrogen atom claim 7 , and said Y and Z are a single bond.13. A cationic polymerization initiator claim 7 , comprising the compound according to .14. A method for producing an intracellular delivery vehicle claim 13 , comprising performing a radical polymerization reaction involving the cationic polymerization initiator according to claim 13 , a monomer comprising carbon-carbon double bonds claim 13 , and a crosslinker. The present ...

Temperature-sensitive fluorescent probe for introduction into cell

There is provided a method for introducing a temperature-sensitive probe comprising a copolymer, which comprises a thermoresponsive unit and a fluorescent unit, into a cell, and the method using the copolymer further comprising a cationic unit as the temperature-sensitive probe, and the method comprising the step of mixing the copolymer with the cell in a solvent. The copolymer can be preferably used as a fluorescence temperature sensor which measures intracellular temperature since the copolymer has a cationic group and thus enters into a cell without using a special method.

INTRACELLULAR DELIVERY VEHICLE

An intracellular delivery vehicle of which surface is covered by a positive charge, an intracellular delivery complex in which a component or compound desired is loaded in the intracellular delivery vehicle, a temperature-sensitive probe comprising the intracellular delivery complex, and a method for measuring the intracellular temperature by the temperature-sensitive probe are disclosed. The intracellular delivery vehicle is useful on account of its capability of easily delivering the component or compound desired inside the cell without inhibiting cell proliferation. 2. The method according to claim 1 , wherein said Y and Z represent single bond.3. The method according to claim 1 , wherein said R claim 1 , R claim 1 , R claim 1 , and Rare each independently selected from the group consisting of methyl group claim 1 , ethyl group claim 1 , methylcarbonyl group claim 1 , isobutyl group claim 1 , and 2-methyl-2-methoxypropyl group.4. The method according to claim 1 , wherein said Rand Rare a methyl group.5. The method according to claim 1 , wherein said Rand R claim 1 , said Rand R claim 1 , said Rand R claim 1 , said Rand R claim 1 , said Rand R claim 1 , and said Rand R claim 1 , each represent an identical substituent claim 1 , and said Y and Z represent an identical substituent or a single bond.6. The method according to claim 1 , wherein said R claim 1 , R claim 1 , R claim 1 , R claim 1 , R claim 1 , R claim 1 , R claim 1 , and Rare a methyl group claim 1 , and said R claim 1 , R claim 1 , Rand Rare a hydrogen atom claim 1 , and said Y and Z are a single bond.7. A gel particle claim 1 , obtained by the method according to .9. A linear polymer at least one end of which is positively charged claim 8 , obtained by the method according to .15. A temperature-sensitive probe claim 11 , comprising the copolymer according to .16. A method for measuring intracellular temperature claim 11 , comprising the steps of:{'claim-ref': {'@idref': 'CLM-00015', 'claim 15'}, '(a) ...

Intracellular delivery vehicle

細胞内送達ベヒクル

【課題】細胞増殖を妨げずに細胞内に所望の成分または化合物を容易に送達できるベヒクルの提供。【解決手段】表面が陽性荷電で覆われた、細胞内送達ベヒクル。【選択図】なし