METHODS AND MEANS FOR PROTECTING THE SKIN AGAINST PATHOGENIC MICROORGANISMS

Described are microorganisms which are, in a first aspect, able to stimulate the growth of microorganisms of the resident skin microbial flora and which do not stimulate the growth of microorganisms of the transient pathogenic micro flora. In a second aspect microorganisms are described which are able to inhibit the growth of microorganisms of the transient pathogenic skin micro flora and which do not inhibit the growth of microorganisms of the resident skin micro flora. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications. 1. A microorganism which is able to stimulate the growth of one or more microorganisms of the resident skin microbial flora and which does not stimulate the growth of microorganisms of the transient pathogenic micro flora.2Staphylococcus epidermidis.. The microorganism of which is able to stimulate the growth of3Staphylococcus epidermidis. The microorganism of which is able to stimulate growth of in vitro.4Staphylococcus epidermidis. The microorganism of which is able to stimulate growth of in an in situ skin assay.5Staphylococcus aureus.. The microorganism of which does not stimulate the growth of6Lactobacillus.. The microorganism of which is a microorganism belonging to the genus of7LactobacillusLactobacillus paracasei, Lactobacillus brevisLactobacillus fermentum.. The microorganism of claim 6 , wherein said is or8Lactobacillus paracaseiLactobacillus paracaseiparacasei.. The microorganism of claim 7 , wherein the is of the subspecies ssp.9Lactobacillus paracasei, Lactobacillus brevisLactobacillus fermentum. The microorganism of which is selected from the group consisting of or having DSMZ accession number DSM 17248 claim 7 , accession number DSM 17247 claim 7 , accession number DSM 17250 and accession number DSM 17249 or a mutant or derivative thereof claim 7 , wherein said mutant or derivative retains the ability to stimulate the growth of ...

PREPARATION OF 7-DEHYDROCHOLESTEROL AND/OR THE BIOSYNTHETIC INTERMEDIATES AND/OR SECONDARY PRODUCTS THEREOF IN TRANSGENIC ORGANISMS

The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves. 1. A method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms which , compared to the wild type , have an increased activity of at least one of the activities selected from the group consisting of Δ8-Δ7-isomerase activity , Δ5-desaturase activity and Δ24-reductase activity.2. The method of claim 1 , wherein the organisms claim 1 , compared to the wild type claim 1 , have an increased activity of at least two of the activities selected from the group consisting of Δ8-Δ7-isomerase activity claim 1 , Δ5-desaturase activity and Δ24-reductase activity.3. The method of claim 1 , wherein the organisms claim 1 , compared to the wild type claim 1 , have an increased Δ8-Δ7-isomerase activity claim 1 , Δ5-desaturase activity and Δ24-reductase activity.4. The method of claim 1 , wherein the Δ8-Δ7-isomerase activity is increased by increasing claim 1 , compared to the wild type claim 1 , gene expression of a nucleic acid encoding a Δ8-Δ7-isomerase.5. The method of claim 4 , wherein gene expression is increased by introducing into the organism one or more nucleic acids encoding a 48-47-isomerase.6. The method of claim 5 , wherein nucleic acids are introduced claim 5 , which encode proteins comprising the amino acid sequence SEQ. ID. NO. 2 or a sequence derived from this sequence by substitution claim 5 , insertion or deletion of amino acids claim 5 , which is at least 30% identical at the amino acid level with the sequence SEQ. ID. NO. 2 claim 5 , and having the enzyme property of a Δ8-Δ7- ...

Microorganisms inhibiting the formation of foot malodor

Described are microorganisms which are able to inhibit the formation of foot malodor by skin microorganisms. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications.

Microorganisms Inhibiting the Formation of Axillary Malodor

Described are microorganisms which are able to suppress the production of malodorous compounds by axillary bacteria. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications. 1. A microorganism that is able to suppress the release of 3-methyl-2-hexenoic acid or an odorous derivative by axillary bacteria.2. The microorganism of that is able to suppress the release of 3-methyl-2-hexenoic acid or its odorous derivatives by axillary bacteria in at least one of Assays A claim 1 , B or C as follows:Assay (A):(i) mixing the microorganism with a microorganism that is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with an odorless axillary secret;(ii) incubating the mixture under conditions allowing the release of 3-methyl-2-hexenoic acid;(iii) extracting short fatty acids from the supernatant of the mixture; and(iv) detecting odor release by the occurrence of 3-methyl-2-hexenoic acid;orAssay (B):(i) mixing the microorganism with a microorganism that is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with N-α-lauryl-glutamine;(ii) incubating the mixture under conditions allowing the release of laureate derived from N-α-lauryl-glutamine; and(iii) analyzing the supernatant of the mixture for the presence of N-α-lauryl-glutamine;or 'a sniffing assay with the nose.', 'Assay (C)3Corynebacterium.. The microorganism of claim 1 , wherein the 3-methyl-2-hexenoic acid or the odorous derivative is released by a bacterium of the genus4Corynebacterium jeikeium.. The microorganism of claim 3 , wherein the bacterium is5Lactobacillus.. The microorganism of claim 1 , wherein the bacterium belongs to the genus6LactobacillusLactobacillus plantarum, Lactobacillus crispatus, Lactobacillus acidophilusLactobacillus acidophilusLactobacillus delbrückii delbrückii.. The microorganism of claim 5 , wherein the is II claim 5 , III ...

Methods and Means for Protecting the Skin Against Pathogenic Microorganisms

Described are microorganisms which are, in a first aspect, able to to stimulate the growth of microorganisms of the resident skin microbial flora and which do not stimulate the growth of microorganisms of the transient pathogenic micro flora. In a second aspect microorganisms are described which are able to inhibit the growth of microorganisms of the transient pathogenic skin micro flora and which do not inhibit the growth of microorganisms of the resident skin micro flora. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications. 1. A microorganism which is able to stimulate the growth of one or more microorganisms of the resident skin microbial flora and which does not stimulate the growth of microorganisms of the transient pathogenic micro flora.2Staphylococcus epidermidis.. The microorganism of which is able to stimulate the growth of3Staphylococcus epidermidis. The microorganism of which is able to stimulate growth of in vitro.4Staphylococcus epidermidis. The microorganism of which is able to stimulate growth of in an in situ skin assay.5Staphylococcus aureus.. The microorganism of which does not stimulate the growth of6Lactobacillus.. The microorganism of which is a microorganism belonging to the genus of7LactobacillusLactobacillus paracasei, Lactobacillus brevisLactobacillus fermentum.. The microorganism of claim 6 , wherein said is or8Lactobacillus paracaseiLactobacillus paracaseiparacasei.. The microorganism of claim 7 , wherein the is of the subspecies ssp.9Lactobacillus paracasei, Lactobacillus brevisLactobacillus fermentum. The microorganism of which is selected from the group consisting of or having DSMZ accession number DSM 17248 claim 7 , accession number DSM 17247 claim 7 , accession number DSM 17250 and accession number DSM 17249 or a mutant or derivative thereof claim 7 , wherein said mutant or derivative retains the ability to stimulate the growth ...

FOOD COMPOSITION CONTAINING AMINO ACIDS AND COCOA

A food composition described herein contains a protein hydrolysate from a legume and cocoa. The food composition may be in a solid or powder composition, and it may also contain creatine, especially creatine monohydrate. The food composition may also contain at least one B vitamin and/or a zinc compound. 1. Food composition containing:a) a protein hydrolysate from a legume selected from peas, beans and lentils; andb) cocoa.2. Food composition according to claim 1 , which also contains creatine.3. Food composition according to or claim 1 , which also contains at least one B vitamin and/or a zinc compound.4. Food composition according to any one of the preceding claims claim 1 , wherein the protein hydrolysate from a legume is a pea protein hydrolysate.5. Food composition containing:a) hydrolysed pea protein; andb) cocoa.6. Food composition according to claim 5 , which also contains creatine.7. Food composition according to or claim 5 , which also contains at least one B vitamin and/or a zinc compound.8. Food composition claim 5 , especially a drink powder claim 5 , containing:a) 40-80% by weight hydrolysed pea protein;b) 20-50% by weight cocoa. The present invention relates to a food composition that contains a protein hydrolysate from a legume and cocoa.Preferably, the food composition according to the invention, especially when it is a solid or powder-form food composition, also contains creatine, especially creatine monohydrate, more especially Creapure©.More preferably, the food composition according to the invention contains at least one B vitamin and/or a zinc compound.More preferably, the food composition according to the invention contains all 20 standard amino acids in the form of the free amino acid and/or in the form of peptides.In the context of the present invention, the term legume relates to beans, lentils and peas, with preference being given to peas. In the context of the present invention, the term legume does not relate to soya or soybeans, that is ...

Microorganisms inhibiting the formation of foot malodor

Described are microorganisms which are able to inhibit the formation of foot malodor by skin microorganisms. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications.

MICROORGANISMS INHIBITING THE FORMATION OF AXILLARY MALODOR

Described are microorganisms which are able to suppress the production of malodorous compounds by axillary bacteria. Also described are compositions comprising such microorganisms as well as the use of such micoorganisms in cosmetic, prophylactic or therapeutic applications. 1. A microorganism that is able to suppress the release of 3-methyl-2-hexenoic acid or an odorous derivative by axillary bacteria.2. The microorganism of that is able to suppress the release of 3-methyl-2-hexenoic acid or its odorous derivatives by axillary bacteria in at least one of Assays A claim 1 , B or C as follows:Assay (A):(i) mixing the microorganism with a microorganism that is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with an odorless axillary secret;(ii) incubating the mixture under conditions allowing the release of 3-methyl-2-hexenoic acid;(iii) extracting short fatty acids from the supernatant of the mixture; and(iv) detecting odor release by the occurrence of 3-methyl-2-hexenoic acid; orAssay (B):(i) mixing the microorganism with a microorganism that is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with N-α-lauryl-glutamine;(ii) incubating the mixture under conditions allowing the release of laureate derived from N-α-lauryl-glutamine; and(iii) analyzing the supernatant of the mixture for the presence of N-α-lauryl-glutamine;or 'a sniffing assay with the nose.', 'Assay (C)3Corynebacterium.. The microorganism of claim 1 , wherein the 3-methyl-2-hexenoic acid or the odorous derivative is released by a bacterium of the genus4Corynebacterium jeikeium.. The microorganism of claim 3 , wherein the bacterium is5Lactobacillus.. The microorganism of claim 1 , wherein the bacterium belongs to the genus6LactobacillusLactobacillus plantarum, Lactobacillus crispatus, Lactobacillus acidophilusLactobacillus acidophilusLactobacillusdelbrückii. The microorganism of claim 5 , wherein the is II claim 5 , III or delbrückii . ...

FAT SUBSTITUTE COMPOSITIONS

The invention relates to fat replacer compositions with improved mouth feel and smooth texture, a method of making said fat replacer compositions and a method of improving the mouth feel and texture of food compositions by using gum in combination with inulin. 1Cassia. A fat replacer composition comprising inulin and gum.2Cassia. A fat replacer composition of consisting of inulin and gum.3Cassia. A fat replacer composition of comprising a total amount of inulin and gum of from about 0.01% by weight or more to about 100% by weight or less claim 1 , based on the total weight of the fat replacer composition.4Cassia. A fat replacer composition of having a weight ratio of gum to inulin of from about 1:15 to about 1:5.5Cassia. A fat replacer composition of wherein the weight average molecular weight of gum is within the range of about 100 claim 1 ,000 Daltons or more to about 1 claim 1 ,500 claim 1 ,000 Daltons or less.6Cassia. A fat replacer composition of wherein the Brookfield viscosity of gum is from about 100 mPa·s or more to about 500 mPa·s or less at 25° C.7. A fat replacer composition of wherein the polymerization degree of inulin is between about 15 or more and about 70 or less.8. A food claim 1 , fodder or beverage composition comprising the fat replacer composition as defined .9Cassia. A food claim 8 , fodder or beverage composition of comprising a total amount of gum and inulin of from about 0.01% by weight or more to about 20% by weight or less claim 8 , based on the total weight of the food claim 8 , fodder or beverage composition.10. A food claim 9 , fodder or beverage composition of wherein the food or fodder composition is a processed cheese product claim 9 , a yoghurt product claim 9 , a processed meat product claim 9 , an ice-cream product claim 9 , a dessert product claim 9 , a margarine product claim 9 , a spreadable oil or fat claim 9 , a fine food product or a dairy based beverage.11. A method of making the food claim 9 , fodder or beverage ...

MICROORGANISMS INHIBITING THE FORMATION OF FOOT MALODOR

Described are microorganisms which are able to inhibit the formation of foot malodor by skin microorganisms. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications. 1. A microorganism which is able to prevent the generation of foot malodor by skin microorganisms.2. The microorganism of which is able to prevent the generation of foot malodor by skin microorganisms in a sniffing assay.3. The microorganism of which is able to suppress the biosynthesis of isovaleric acid by skin microorganisms.4. The microorganism of claim 3 , which is able to suppress the biosynthesis of isovaleric acid by skin microorganisms in an assay comprising the following steps:(i) mixing said microorganism with a microorganism which is capable of generating isovaleric acid and with a precursor of isovaleric acid;(ii) incubating the mixture under conditions allowing the generation of isovaleric acid;(iii) extracting short fatty acids from the supernatant of the mixture; and(iv) detecting the generation of foot malodor by the occurrence of isovaleric acid.5MicrococcusPropionibacterium.. The microorganism of claim 3 , wherein isovaleric acid is generated by a bacterium of the genus or6PropionibacteriumPropionibacterium freudenreichii.. The microorganism of claim 5 , wherein the is7. The microorganism of claim 1 , which is able to inhibit the growth of foot malodor generating skin microorganisms.8. The microorganism of claim 7 , which is able to inhibit the growth of foot malodor generating skin microorganisms in an inhibition assay.9Lactobacillus, LactococcusLeuconostoc.. The microorganism of claim 1 , which belongs to a genus selected from the group consisting of and10Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus curvatus, Lactobacillus delbrückii delbrückiiLeuconostoc mesenteroides.. The microorganism of claim 9 , which is selected from the group consisting of claim 9 , ...

Microorganisms Inhibiting the Formation of Axillary Malodor

Described are microorganisms which are able to suppress the production of malodorous compounds by axillary bacteria. Also described are compositions comprising such microorganisms as well as the use of such micoorganisms in cosmetic, prophylactic or therapeutic applications. 1. A microorganism that is able to suppress the release of 3-methyl-2-hexenoic acid or an odorous derivative by axillary bacteria.2. The microorganism of that is able to suppress the release of 3-methyl-2-hexenoic acid or its odorous derivatives by axillary bacteria in at least one of Assays A claim 1 , B or C as follows:{'claim-text': ['(i) mixing the microorganism with a microorganism that is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with an odorless axillary secret;', '(ii) incubating the mixture under conditions allowing the release of 3-methyl-2-hexenoic acid;', '(iii) extracting short fatty acids from the supernatant of the mixture; and', '(iv) detecting odor release by the occurrence of 3-methyl-2-hexenoic acid;'], '#text': 'Assay (A):'}or{'claim-text': ['(i) mixing the microorganism with a microorganism that is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with N-α-lauryl-glutamine;', '(ii) incubating the mixture under conditions allowing the release of laureate derived from N-α-lauryl-glutamine; and', '(iii) analyzing the supernatant of the mixture for the presence of N-α-lauryl-glutamine;'], '#text': 'Assay (B):'}or{'claim-text': 'a sniffing assay with the nose.', '#text': 'Assay (C):'}3Corynebacterium.. The microorganism of claim 1 , wherein the 3-methyl-2-hexenoic acid or the odorous derivative is released by a bacterium of the genus4Corynebacterium jeikeium.. The microorganism of claim 3 , wherein the bacterium is5Lactobacillus.. The microorganism of claim 1 , wherein the bacterium belongs to the genus6LactobacillusLactobacillus plantarum, Lactobacillus crispatus, Lactobacillus acidophilus II, ...

FOOD COMPOSITION CONTAINING AMINO ACIDS AND COCOA

The present invention relates to a food composition which contains a protein hydrolysate from a legume and cocoa. 1. Food composition containing:a) a protein hydrolysate from a legume selected from peas, beans and lentils; andb) cocoa.2. Food composition according to claim 1 , which also contains creatine.3. Food composition according to or claim 1 , which also contains at least one B vitamin and/or a zinc compound.4. Food composition according to any one of the preceding claims claim 1 , wherein the protein hydrolysate from a legume is a pea protein hydrolysate.5. Food composition containing:a) hydrolysed pea protein; andb) cocoa.6. Food composition according to claim 5 , which also contains creatine.7. Food composition according to or claim 5 , which also contains at least one B vitamin and/or a zinc compound.8. Food composition claim 5 , especially a drink powder claim 5 , containing:a) 40-80% by weight hydrolysed pea protein;b) 20-50% by weight cocoa. The present invention relates to a food composition that contains a protein hydrolysate from a legume and cocoa.Preferably, the food composition according to the invention, especially when it is a solid or powder-form food composition, also contains creatine, especially creatine monohydrate, more especially Creapure©.More preferably, the food composition according to the invention contains at least one B vitamin and/or a zinc compound.More preferably, the food composition according to the invention contains all 20 standard amino acids in the form of the free amino acid and/or in the form of peptides.In the context of the present invention, the term legume relates to beans, lentils and peas, with preference being given to peas. In the context of the present invention, the term legume does not relate to soya or soybeans, that is to say soya and soybeans are excluded from the present invention.In accordance with a further preferred embodiment, the food composition according to the invention contains hydrolysed pea ...

MEANS AND METHODS FOR PREVENTING AND/OR TREATING CARIES

The present invention relates to a microorganism belonging to the group of lactic acid bacteria characterized in that it is capable of specifically binding to , wherein the specific binding is (i) resistant to heat treatment; and/or (ii) resistant to protease treatment; and/or (iii) calcium-dependent; and/or (iv) formed within a pH range between 4.5 and 8.5; and/or (v) formed in the presence of saliva. Preferably, the specific binding can be assayed as follows: 133-. (canceled)35. The composition of claim 34 , wherein the feed composition is a pet feed for dogs claim 34 , cats claim 34 , and rats claim 34 , a cattle feed for cows and pigs claim 34 , chicken feed for chicken and turkeys claim 34 , or a fish cultivation feed for porgy and yellowtail.36. The composition of claim 34 , wherein the at least one orally acceptable carrier or excipient comprises a raw feed material selected from the group consisting of cereals claim 34 , brans claim 34 , oil-seed meals claim 34 , animal-derived raw feed materials claim 34 , and combinations thereof.37. The composition of claim 34 , wherein the at least one orally acceptable carrier or excipient comprises a cereal selected from the group consisting of mile claim 34 , wheat claim 34 , barley claim 34 , oats claim 34 , rye claim 34 , brown rice claim 34 , buckwheat claim 34 , fox-tail millet claim 34 , Chinese millet claim 34 , Deccan grass claim 34 , corn claim 34 , soybean claim 34 , and combinations thereof.38. The composition of claim 34 , wherein the at least one orally acceptable carrier or excipient comprises a bran selected from the group consisting of rice bran claim 34 , defatted rice bran claim 34 , bran claim 34 , lowest-grade flour claim 34 , wheat germ claim 34 , barley bran claim 34 , screening pellet claim 34 , corn bran claim 34 , corn germ claim 34 , and combinations thereof.39. The composition of claim 34 , wherein the at least one orally acceptable carrier or excipient comprises an oil-seed meal selected ...

Meat Paste Compositions Having Improved Creaminess

The invention relates to spreadable meat paste compositions which are improved in their creaminess, a method of making said meat paste compositions and a method of improving the creaminess of meat paste compositions by using Cassia gum. 1. A meat paste composition comprising a meat base and from about 0.01 to about 0.5% by weight of active Cassia gum , based on the total weight of the meat paste composition.2. The meat paste composition of claim 1 , wherein the meat base is derived from pork claim 1 , beef claim 1 , poultry claim 1 , fish or blends thereof.3. The meat paste composition of claim 2 , wherein the meat base comprises up to about 50% by weight of animal fat claim 2 , up to about 100% by weight of meat (meat protein) and up to 60% by weight of water claim 2 , based on the weight of the meat base.4. The meat paste composition of comprising from about 25 to 95% by weight of meat base claim 1 , based on the total weight of the meat paste composition.5. The meat paste composition of further comprising additives/ingredients.6. The meat base of claim 5 , wherein the total amount of the additives/ingredients is from about 2 to about 15% by weight claim 5 , based on the total weight of the meat paste composition.7. The meat paste composition of further comprising liver selected from pork liver claim 1 , poultry liver and beef liver or fish liver or blends thereof.8. The meat paste composition of comprising up to about 30% by weight of liver.9. The meat past composition of wherein the Cassia gum is obtained from the endosperm of the seeds of Cassia tora claim 1 , Cassia obtusifolia claim 1 , and combinations thereof10. A method of making the meat paste composition as defined in claim 1 , said method comprising the steps of(i) providing the meat base,(ii) adding Cassia gum and optional additives/ingredients,(iii) blending the mixture, and(iv) treating the mixture by heat.11. A method of using Cassia gum for improving the creaminess of a spreadable meat paste ...

Methods And Means For Protecting The Skin Against Pathogenic Microorganisms

Described are microorganisms which are, in a first aspect, able to to stimulate the growth of microorganisms of the resident skin microbial flora and which do not stimulate the growth of microorganisms of the transient pathogenic micro flora. In a second aspect microorganisms are described which are able to inhibit the growth of microorganisms of the transient pathogenic skin micro flora and which do not inhibit the growth of microorganisms of the resident skin micro flora. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications. 1. A microorganism which is able to stimulate the growth of one or more microorganisms of the resident skin microbial flora and which does not stimulate the growth of microorganisms of the transient pathogenic micro flora.2. The microorganism of claim 1 , wherein said microorganism:{'i': 'Staphylococcus epidermidis;', 'i) is able to stimulate the growth of'}{'i': 'Staphylococcus epidermidis', 'ii) is able to stimulate growth of in vitro;'}{'i': 'Staphylococcus epidermidis', 'iii) is able to stimulate growth of in an in situ skin assay;'}{'i': 'Staphylococcus aureus;', 'iv) does not stimulate the growth of'}{'i': 'Lactobacillus;', 'v) is a microorganism belonging to the genus of'}{'i': Lactobacillus paracasei, Lactobacillus brevis', 'Lactobacillus fermentum;, 'vi) is or'}{'i': Lactobacillus paracasei', 'Paracasei, 'vii) ssp. ; or'}{'i': Lactobacillus paracasei, Lactobacillus brevis', 'Lactobacillus fermentum, 'viii) is selected from the group consisting of or having DSMZ accession number DSM 17248, accession number DSM 17247, accession number DSM 17250 and accession number DSM 17249 or a mutant or derivative thereof, wherein said mutant or derivative retains the ability to stimulate the growth of at least one microorganism of the resident skin microbial flora and does not stimulate the growth of microorganisms of the transient pathogenic ...

Microorganisms inhibiting the formation of foot malodor

Described are microorganisms which are able to inhibit the formation of foot malodor by skin microorganisms. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications.

PREPARATION OF 7-DEHYDROCHOLESTEROL AND/OR THE BIOSYNTHETIC INTERMEDIATES AND/OR SECONDARY PRODUCTS THEREOF IN TRANSGENIC ORGANISMS

The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves. 1. A method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof comprising the step of culturing organisms which , compared to the wild type , have an increased activity of at least one of the activities selected from the group consisting of Δ8-Δ7-isomerase activity , Δ5-desaturase activity and Δ24-reductase activity.2. The method of claim 1 , wherein the organisms claim 1 , compared to the wild type claim 1 , have an increased activity of at least two of the activities selected from the group consisting of Δ8-Δ7-isomerase activity claim 1 , Δ5-desaturase activity and Δ24-reductase activity.3. The method of claim 1 , wherein the organisms claim 1 , compared to the wild type claim 1 , have an increased Δ8-Δ7-isomerase activity claim 1 , Δ5-desaturase activity and Δ24-reductase activity.4. The method of claim 1 , further comprising the step of increasing the Δ8-Δ7-isomerase activity by increasing claim 1 , compared to the wild type claim 1 , gene expression of a nucleic acid encoding a Δ8-Δ7-isomerase.5. The method of claim 4 , wherein the gene expression is increased by introducing into the organism one or more nucleic acids encoding a Δ8-Δ7-isomerase.6. The method of claim 5 , further comprising the step of introducing the nucleic acids claim 5 , which encode proteins comprising the amino acid sequence SEQ. ID. NO. 2 or a sequence derived from this sequence by substitution claim 5 , insertion or deletion of amino acids claim 5 , which is at least 30% identical at the amino acid level with the sequence ...

Methods and means for protecting the skin against pathogenic microorganisms

Described are microorganisms which are, in a first aspect, able to stimulate the growth of microorganisms of the resident skin microbial flora and which do not stimulate the growth of microorganisms of the transient pathogenic micro flora. In a second aspect microorganisms are described which are able to inhibit the growth of microorganisms of the transient pathogenic skin micro flora and which do not inhibit the growth of microorganisms of the resident skin micro flora. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications.

MICROORGANISMS INHIBITING THE FORMATION OF AXILLARY MALODOR

Described are microorganisms which are able to suppress the production of malodorous compounds by axillary bacteria. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications. 1. A microorganism that is able to suppress the release of 3-methyl-2-hexenoic acid or an odorous derivative by axillary bacteria.2. The microorganism of that is able to suppress the release of 3-methyl-2-hexenoic acid or its odorous derivatives by axillary bacteria in at least one of Assays A claim 1 , B or C as follows:Assay (A):(i) mixing the microorganism with a microorganism that is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with an odorless axillary secret;(ii) incubating the mixture under conditions allowing the release of 3-methyl-2-hexenoic acid;(iii) extracting short fatty acids from the supernatant of the mixture; and(iv) detecting odor release by the occurrence of 3-methyl-2-hexenoic acid;orAssay (B):(i) mixing the microorganism with a microorganism that is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with N-α-lauryl-glutamine;(ii) incubating the mixture under conditions allowing the release of laureate derived from N-α-lauryl-glutamine; and(iii) analyzing the supernatant of the mixture for the presence of N-α-lauryl-glutamine;or 'a sniffing assay with the nose.', 'Assay (C)3Corynebacterium.. The microorganism of claim 1 , wherein the 3-methyl-2-hexenoic acid or the odorous derivative is released by a bacterium of the genus4Corynebacterium jeikeium.. The microorganism of claim 3 , wherein the bacterium is5Lactobacillus.. The microorganism of claim 1 , wherein the bacterium belongs to the genus6LactobacillusLactobacillus plantarum, Lactobacillus crispatus, Lactobacillus acidophilusLactobacillus acidophilusLactobacillus delbrückii delbrückii.. The microorganism of claim 5 , wherein the is II claim 5 , III ...

Microorganisms inhibiting the formation of axillary malodor

Described are microorganisms which are able to suppress the production of malodorous compounds by axillary bacteria. Also described are compositions comprising such microorganisms as well as the use of such micoorganisms in cosmetic, prophylactic or therapeutic applications.

Means and methods for preventing and/or treating caries

The present invention relates to a microorganism belonging to the group of lactic acid bacteria characterized in that it is capable of specifically binding to Streptococcus mutans , wherein the specific binding is (i) resistant to heat treatment; and/or (ii) resistant to protease treatment; and/or (iii) calcium-dependent; and/or (iv) formed within a pH range between 4.5 and 8.5; and/or (v) formed in the presence of saliva. Preferably, the specific binding can be assayed as follows: (a) growing said microorganism to stationary phase; (b) mixing said microorganism with Streptococcus mutans which has been grown to stationary phase; (c) incubating the mixture obtained in step (b) under conditions allowing the formation of aggregates of said microorganism and Streptococcus mutans and (d) detecting aggregates by the occurrence of a pellet. Another aspect of the present invention is an analog or fragment of said microorganism which is thermally inactivated or lyophilized, wherein said analog or fragment retains the capability of specifically binding to Streptococcus mutans . In addition, the present invention encompasses compositions and additives for food, feed or drinks comprising the microorganism belonging to the group of lactic acid bacteria which specifically bind to Streptococcus mutans or an analog or fragment thereof. Moreover, uses of said microorganism or said analog or fragment thereof for the preparation of an anticariogenic or pharmaceutical composition or anticariogenic food or feedstuff as well as methods for producing said compositions or food or feedstuff are provided by the present invention.

MICROORGANISMS INHIBITING THE FORMATION OF FOOT MALODOR

Described are microorganisms which are able to inhibit the formation of foot malodor by skin microorganisms. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications.

PREPARATION OF 7-DEHYDROCHOLESTEROL AND/OR THE BIOSYNTHETIC INTERMEDIATES AND/OR SECONDARY PRODUCTS THEREOF IN TRANSGENIC ORGANISMS

The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves. 1. A method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms which , compared to the wild type , have an increased activity of at least one of the activities selected from the group consisting of Δ8-Δ7-isomerase activity , Δ5-desaturase activity and Δ24-reductase activity.2. The method of claim 1 , wherein the organisms claim 1 , compared to the wild type claim 1 , have an increased activity of at least two of the activities selected from the group consisting of Δ8-Δ7-isomerase activity claim 1 , Δ5-desaturase activity and Δ24-reductase activity.3. The method of claim 1 , wherein the organisms claim 1 , compared to the wild type claim 1 , have an increased Δ8-Δ7-isomerase activity claim 1 , Δ5-desaturase activity and Δ24-reductase activity.4. The method of claim 1 , wherein the Δ8-Δ7-isomerase activity is increased by increasing claim 1 , compared to the wild type claim 1 , gene expression of a nucleic acid encoding a Δ8-Δ7-isomerase.5. The method of claim 4 , wherein gene expression is increased by introducing into the organism one or more nucleic acids encoding a Δ8-Δ7-isomerase.6. The method of claim 5 , wherein nucleic acids are introduced claim 5 , which encode proteins comprising the amino acid sequence SEQ. ID. NO. 2 or a sequence derived from this sequence by substitution claim 5 , insertion or deletion of amino acids claim 5 , which is at least 30% identical at the amino acid level with the sequence SEQ. ID. NO. 2 claim 5 , and having the enzyme property of a Δ8-Δ7- ...

Methods And Means For Protecting The Skin Against Pathogenic Microorganisms

Described are microorganisms which are, in a first aspect, able to to stimulate the growth of microorganisms of the resident skin microbial flora and which do not stimulate the growth of microorganisms of the transient pathogenic micro flora. In a second aspect microorganisms are described which are able to inhibit the growth of microorganisms of the transient pathogenic skin micro flora and which do not inhibit the growth of microorganisms of the resident skin micro flora. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications. 1. A microorganism which is able to stimulate the growth of one or more microorganisms of the resident skin microbial flora and which does not stimulate the growth of microorganisms of the transient pathogenic micro flora.2. The microorganism of claim 1 , wherein said microorganism:{'i': 'Staphylococcus epidermidis;', 'i) is able to stimulate the growth of'}{'i': 'Staphylococcus epidermidis', 'ii) is able to stimulate growth of in vitro;'}{'i': 'Staphylococcus epidermidis', 'iii) is able to stimulate growth of in an in situ skin assay;'}{'i': 'Staphylococcus aureus;', 'iv) does not stimulate the growth of'}{'i': 'Lactobacillus;', 'v) is a microorganism belonging to the genus of'}{'i': Lactobacillus paracasei, Lactobacillus brevis', 'Lactobacillus fermentum;, 'vi) is or'}{'i': Lactobacillus paracasei', 'Paracasei, 'vii) ssp. ; or'}{'i': Lactobacillus paracasei, Lactobacillus brevis', 'Lactobacillus fermentum, 'viii) is selected from the group consisting of or having DSMZ accession number DSM 17248, accession number DSM 17247, accession number DSM 17250 and accession number DSM 17249 or a mutant or derivative thereof, wherein said mutant or derivative retains the ability to stimulate the growth of at least one microorganism of the resident skin microbial flora and does not stimulate the growth of microorganisms of the transient pathogenic ...

Method for producing ergosta-5,7-dienol and/or biosynthetic intermediate and/or secondary products thereof in transgenic organisms

The invention relates to a method for producing ergosta-5,7-dienol and/or biosynthetic intermediate and/or secondary products thereof by cultivating genetically modified organisms. The invention also relates to the genetically modified organisms themselves, particularly yeasts.

Method for the production of 7-dehydrocholesterol and/or the biosynthetic intermediate or subsequent products thereof in transgenic organisms

The invention relates to a method for the production of 7-dehydrocholesterol and/or the biosynthetic intermediate or subsequent products thereof by the cultivation of organisms, in particular, yeasts which show an increased activity of at least one activity, selected from delta-8/delta-7 isomerisation activity, delta-5 desaturase activity or delta-24 reductase activity. The invention further relates to the nucleic acid constructs necessary for production of the genetically-modified organisms and the genetically-modified organisms, in particular, the yeasts themselves.

Microorganism for the production of succinic acid

Die Erfindung betrifft einen isolierten genetisch veränderten Mikroorganismus, wobei das Gen IDH1 sowie zumindest eines der Gene SDH2 und DIC1 unter der Kontrolle eines ersten Promotors steht, welcher durch einen Kultivierungszusatz zu einem Wachstumskulturmedium reprimiert wird und in Abwesenheit des Kultivierungszusatzes aktiv ist, wobei die Gene der Gruppe "PYC1, ACS1, CIT1, ACO1, ICL1, MSL1 und CIT2 optional auch MDH3" konstitutiv aktiv sind, sowie Verwendungen eines solchen Mikroorganismus, insbesondere zur Herstellung von Bernsteinsäure. The invention relates to an isolated genetically modified microorganism, wherein the gene IDH1 and at least one of the genes SDH2 and DIC1 is under the control of a first promoter, which is repressed by a cultivating addition to a growth culture medium and is active in the absence of the cultivation additive, the genes of the Group "PYC1, ACS1, CIT1, ACO1, ICL1, MSL1 and CIT2 optionally also MDH3" constitutively active, as well as uses of such a microorganism, in particular for the production of succinic acid.

Probiotic microorganisms for the reduction of manure odor

Described are microorganism which are able to reduce the generation of feces odor by decreasing the amount of at least one of the compounds methyl mercaptan, a sulphide compound, cadavarine, putrescine, indole or skatole, and wherein said decrease in the amount of said compounds is independent of the growth of the microorganism. Also described are compositions, comprising such microorganisms, e.g. food, feed or pharmaceutical compositions and the use of such microorganisms for suppressing feces odor or the preparation of foodstuff or feedstuff, as well as corresponding methods for the production of food or feed composition and additives for food, feed or drinks.

Means and methods for preventing and/or treating caries

Probiotic microorganisms for the reduction of manure odor

Described are microorganism which are able to reduce the generation of feces odor by decreasing the amount of at least one of the compounds methyl mercaptan, a sulphide compound, cadavarine, putrescine, indole or skatole, and wherein said decrease in the amount of said compounds is independent of the growth of the microorganism. Also described are compositions, comprising such microorganisms, e.g. food, feed or pharmaceutical compositions and the use of such microorganisms for suppressing feces odor or the preparation of foodstuff or feedstuff, as well as corresponding methods for the production of food or feed composition and additives for food, feed or drinks.

Microorganism for the production of succinic acid

The invention relates to an isolated genetically modified microorganism in which the gene IDH1 and at least one of the genes SDH2 and DIC1 are under the control of a first promoter that is repressed to a growth culture medium by means of a cultivation additive and is active in the absence of the cultivation additive. The genes that are part of the group comprising "PYC1, ACS1, CIT1, ACO1, ICL1, MSL1, and CIT2, optionally also MDH3" are constitutively active. The invention further relates to uses of such a microorganism, especially for producing succinic acid.

Process for the production of Ergosta-5,7-dienol and / or its biosynthetic intermediate and / or secondary products in transgenic organisms

Die vorliegende Erfindung betrifft ein Verfahren von Ergosta-5,7-dienol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten durch Kultivierung von genetisch veränderten Organismen sowie die genetisch veränderten Organismen, insbesondere Hefen selbst. The present invention relates to a method of Ergosta-5,7-dienol and / or its biosynthetic intermediate and / or secondary products by cultivating genetically modified organisms and the genetically modified organisms, in particular yeasts themselves.

Uses and methods for preventing and/or treating caries caused by mutans streptococci

The present invention relates to the use of a microorganism belonging to the group of lactic acid bacteria or a mutant or derivative thereof, characterized in that it is capable of specifically binding to a bacterium belonging to the group of mutans Streptococci, wherein the specific binding is (i) resistant to heat treatment; and/or (ii) resistant to protease treatment; and/or (iii) calcium-dependent; and/or (iv) formed within a pH range between 4.5 and 8.5; and/or (v) formed in the presence of saliva, for the preparation of an anticariogenic composition for the treatment or prevention of caries caused by mutans Streptococci other than Streptococcus mutans. Preferably, the specific binding can be assayed as follows: (a) growing said microorganism to stationary phase; (b) mixing said microorganism with a bacterium belonging to the group of mutans Streptococci which has been grown to stationary phase; (c) incubating the mixture obtained in step (b) under conditions allowing the formation of aggregates of said microorganism and a bacterium of the group of mutans Streptococci; and (d) detecting aggregates by the occurrence of a pellet. Another aspect of the present invention is a method of prophylaxis or treatment of caries caused by mutans Streptococci other than Streptococcus mutans, comprising administering a microorganism belonging to the group of lactic acid bacteria characterized in that said microorganism is capable of specifically binding to a bacterium belonging to the group of mutans Streptococci or a mutant, derivative, analog or fragment of said microorganism.

Means and Methods for Preventing and/or Treating Caries

The present invention relates to a microorganism belonging to the group of lactic acid bacteria characterized in that it is capable of specifically binding to Streptococcus mutans, wherein the specific binding is (i) resistant to heat treatment; and/or (ii) resistant to protease treatment; and/or (iii) calcium-dependent; and/or (iv) formed within a pH range between 4.5 and 8.5; and/or (v) formed in the presence of saliva. Preferably, the specific binding can be assayed as follows: (a) growing said microorganism to stationary phase; (b) mixing said microorganism with Streptococcus mutans which has been grown to stationary phase; (c) incubating the mixture obtained in step (b) under conditions allowing the formation of aggregates of said microorganism and Streptococcus mutans and (d) detecting aggregates by the occurrence of a pellet. Another aspect of the present invention is an analog or fragment of said microorganism which is thermally inactivated or lyophilized, wherein said analog or fragment retains the capability of specifically binding to Streptococcus mutans. In addition, the present invention encompasses compositions and additives for food, feed or drinks comprising the microorganism belonging to the group of lactic acid bacteria which specifically bind to Streptococcus mutans or an analog or fragment thereof. Moreover, uses of said microorganism or said analog or fragment thereof for the preparation of an anticariogenic or pharmaceutical composition or anticariogenic food or feedstuff as well as methods for producing said compositions or food or feedstuff are provided by the present invention.

Compositions, kits and uses for protecting the skin against pathogenic microorganisms

Described are compositions and kits comprising: (i) microorganisms which are able to stimulate the growth of microorganisms of the resident skin microbial flora and which do not stimulate the growth of microorganisms of the transient pathogenic micro flora and (ii) microorganisms which are able to inhibit the growth of one or more microorganisms of the transient pathogenic skin micro flora and which do not inhibit the growth of microorganisms of the healthy normal resident skin micro flora, in order to protect the skin against pathogenic microorganisms and to treat skin diseases. The present invention also relates to uses of the above mentioned microorganisms and to methods for the production of compositions and kits comprising such microorganisms.

Microorganism for the production of succinic acid

The invention relates to an isolated genetically modified microorganism in which the gene IDH1 and at least one of the genes SDH2 and DIC1 are under the control of a first promoter that is repressed to a growth culture medium by means of a cultivation additive and is active in the absence of the cultivation additive. The genes that are part of the group comprising 'PYC1, ACS1, CIT1, ACO1, ICL1, MSL1, and CIT2, optionally also MDH3' are constitutively active. The invention further relates to uses of such a microorganism, especially for producing succinic acid.

Use and methods for preventing and/or treating oral malodour

Uses and methods for preventing and/or treating oral malodour

Described is a microorganism belonging to the group of lactic acid bacteria which is able to drastically reduce the peptide concentration in saliva thereby depleting the substrate used by anaerobic microorganisms of the oral micro-flora which are the causative agent for oral malodour. Moreover, described is a microorganism belonging to the group of lactic acid bacteria which is able to stimulate the growth of Streptococcus salivarius but does not stimulate the growth of Streptococcus mutans and/or Porphyromonas gingivalis. Also described are compositions containing the above-mentioned microorganisms, their use for preventing and/or treating oral malodour and/or halitosis and to methods for preventing and/or treating oral malodour and/or halitosis.

MEANS AND METHODS TO AVOID AND / OR TREAT CARIES.

Un microorganismo que pertenece al género de Lactobacillus caracterizado porque es capaz de unirse específicamente a Streptococcus mutans, en donde la unión específica es (i)resistente a un tratamiento de calor a más de 95ºC durante por lo menos 20 minutos, y (ii)resistente al tratamiento de proteasa con una proteasa seleccionada del grupo que consisten en pronasa E, proteinasa K, la tripsina y quimotripsina, y (iii)dependiente de calcio, y (iv)se forma en un rango de pH entre 4.5 y 8.5, y (v)se forma en la presencia de saliva. A microorganism that belongs to the genus of Lactobacillus characterized in that it is capable of specifically binding to Streptococcus mutans, where the specific binding is (i) resistant to a heat treatment at more than 95 ° C for at least 20 minutes, and (ii) resistant to protease treatment with a protease selected from the group consisting of pronase E, proteinase K, trypsin and chymotrypsin, and (iii) calcium dependent, and (iv) is formed in a pH range between 4.5 and 8.5, and ( v) is formed in the presence of saliva.

Microorganism for the production of succinic acid

The invention relates to an isolated genetically modified microorganism in which the gene IDH1 and at least one of the genes SDH2 and DIC1 are under the control of a first promoter that is repressed to a growth culture medium by means of a cultivation additive and is active in the absence of the cultivation additive. The genes that are part of the group comprising “PYC1, ACS1, CIT1, ACO1, ICL1, MSL1, and CIT2, optionally also MDH3” are constitutively active. The invention further relates to uses of such a microorganism, especially for producing succinic acid.

Uses and methods for preventing and/or treating caries caused by mutants streptococci

The present invention relates to the use of a microorganism belonging to the group of lactic acid bacteria or a mutant or derivative thereof, characterized in that it is capable of specifically binding to a bacterium belonging to the group of mutans Streptococci, wherein the specific binding is (i) resistant to heat treatment; and/or (ii) resistant to protease treatment; and/or (iii) calcium-dependent; and/or (iv) formed within a pH range between 4.5 and 8.5; and/or (v) formed in the presence of saliva, for the preparation of an anticariogenic composition for the treatment or prevention of caries caused by mutans Streptococci other than Streptococcus mutans. Preferably, the specific binding can be assayed as follows: (a) growing said microorganism to stationary phase; (b) mixing said microorganism with a bacterium belonging to the group of mutans Streptococci which has been grown to stationary phase; (c) incubating the mixture obtained in step (b) under conditions allowing the formation of aggregates of said microorganism and a bacterium of the group of mutans Streptococci; and (d) detecting aggregates by the occurrence of a pellet. Another aspect of the present invention is a method of prophylaxis or treatment of caries caused by mutans Streptococci other than Streptococcus mutans, comprising administering a microorganism belonging to the group of lactic acid bacteria characterized in that said microorganism is capable of specifically binding to a bacterium belonging to the group of mutans Streptococci or a mutant, derivative, analog or fragment of said microorganism.

New strains of Lactobacillus and its use against Helicobacter pylori

Uso de células de Lactobacillus, tal como están depositadas bajo DSM 17646, DSM 17647, DSM 17648, DSM17649, DSM 17650, DSM 17651, DSM 17652 o DSM 17653, para la preparación de una composición farmacéuticao dietética para la profilaxis y/o el tratamiento de enfermedades condicionadas por la infección con Helicobacterpylori, en particular enfermedades gastrointestinales tales como úlceras gástricas, estando las células deLactobacillus en condiciones de agregarse a Helicobacter pylori bajo las condiciones de cultivo del tracto digestivohumano, en particular del estómago. Use of Lactobacillus cells, as deposited under DSM 17646, DSM 17647, DSM 17648, DSM17649, DSM 17650, DSM 17651, DSM 17652 or DSM 17653, for the preparation of a dietary pharmaceutical composition for prophylaxis and / or treatment of diseases conditioned by infection with Helicobacterpylori, in particular gastrointestinal diseases such as gastric ulcers, with Lactobacillus cells being able to be added to Helicobacter pylori under culture conditions of the human digestive tract, particularly the stomach.

Uses and methods for preventing and/or treating caries caused by mutants streptococci

The present invention relates to the use of a microorganism belonging to the group of lactic acid bacteria or a mutant or derivative thereof, characterized in that it is capable of specifically binding to a bacterium belonging to the group of mutans Streptococci, wherein the specific binding is (i) resistant to heat treatment; and/or (ii) resistant to protease treatment; and/or (iii) calcium-dependent; and/or (iv) formed within a pH range between 4.5 and 8.5; and/or (v) formed in the presence of saliva, for the preparation of an anticariogenic composition for the treatment or prevention of caries caused by mutans Streptococci other than Streptococcus mutans . Preferably, the specific binding can be assayed as follows: (a) growing said microorganism to stationary phase; (b) mixing said microorganism with a bacterium belonging to the group of mutans Streptococci which has been grown to stationary phase; (c) incubating the mixture obtained in step (b) under conditions allowing the formation of aggregates of said microorganism and a bacterium of the group of mutans Streptococci; and (d) detecting aggregates by the occurrence of a pellet. Another aspect of the present invention is a method of prophylaxis or treatment of caries caused by mutans Streptococci other than Streptococcus mutans , comprising administering a microorganism belonging to the group of lactic acid bacteria characterized in that said microorganism is capable of specifically binding to a bacterium belonging to the group of mutans Streptococci or a mutant, derivative, analog or fragment of said microorganism.

Preparation of 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof in transgenic organisms

The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.

Means and methods for preventing and/or treating caries

The present invention relates to a microorganism belonging to the group of lactic acid bacteria characterized in that it is capable of specifically binding to Streptococcus mutans, wherein the specific binding is (i) resistant to heat treatment; and/or (ii) resistant to protease treatment; and/or (iii) calcium-dependent; and/or (iv) formed within a pH range between 4.5 and 8.5; and/or (v) formed in the presence of saliva. Preferably, the specific binding can be assayed as follows: (a) growing said microorganism to stationary phase; (b) mixing said microorganism with Streptococcus mutans which has been grown to stationary phase; (c) incubating the mixture obtained in step (b) under conditions allowing the formation of aggregates of said microorganism and Streptococcus mutans and (d) detecting aggregates by the occurrence of a pellet. Another aspect of the present invention is an analog or fragment of said microorganism which is thermally inactivated or lyophilized, wherein said analog or fragment retains the capability of specifically binding to Streptococcus mutans. In addition, the present invention encompasses compositions and additives for food, feed or drinks comprising the microorganism belonging to the group of lactic acid bacteria which specifically bind to Streptococcus mutans or an analog or fragment thereof. Moreover, uses of said microorganism or said analog or fragment thereof for the preparation of an anticariogenic or pharmaceutical composition or anticariogenic food or feedstuff as well as methods for producing said compositions or food or feedstuff are provided by the present invention.

Mittel und verfahren zur vorbeugung und/oder behandlung von karies

Food composition containing amino acids and cocoa

The present invention relates to a food composition which contains a protein hydrolysate from a legume and cocoa.

Levnedsmiddelsammensætning indeholdende aminosyrer og cacao

Method for the production of zymosterol and/or the biosynthetic intermediate and/or subsequent products thereof in transgenic organisms

Method for the production of 7-dehydrocholesterol and/or the biosynthetic intermediate or subsequent products thereof in transgenic organisms