Enrichment and domestication method of hydrogen nutrition type bacteria groups producing methane

The invention relates to an enrichment and domestication method of hydrogen nutrition type bacteria groups producing methane. The domestication method that a marsh gas fermentation system runs at constant temperature and constant pressure, H2 and CO2 are added through an external source, and gas circulation is conducted is designed, and the growth and enrichment of the hydrogen nutrition type bacteria groups producing methane in marsh gas fermentation activated sludge are promoted. By means of the method, the content of the hydrogen nutrition type bacteria groups producing methane in methanogens can be effectively increased, and the marsh gas fermentation activated sludge rich in the hydrogen nutrition type bacteria groups producing methane can be obtained.

Production process of neurotrophic factor

The present invention discloses the production process of neurotrophic factor. The production process includes computer-aided design, modifying and altering the gene of ciliary nerve trophic factor to obtain modified mutation human ciliary nerve trophic factor cDNA, artificially synthesizing the cDNA, constituting recombinant plasmid, converting to bacillus coli, high density expression in automatic fermentation tank; renaturation in tangent flow superfiltering mode; ion exchange chromatography, hydrophobic chromatography and molecular sieve chromatography to purify and thus to obtain high-purity ciliary nerve trophic factor. The present invention has the advantages of stable and efficient expression of mutant in bacillus coli, less polarization insuperfiltering course and high purity of renaturated ciliary nerve trophic factor.

Preparation method of positive electrode active material, positive electrode active material, positive electrode plate and electrode assembly

The invention provides a preparation method of a positive electrode active material, the positive electrode active material, a positive electrode plate and an electrode assembly, and belongs to the field of new material preparation and energy storage. The preparation method of the positive electrode active material comprises the following steps: weighing acetate and/or oxalate corresponding to each metal element according to a general formula, and mixing to obtain a metal salt mixture; mixing the metal salt mixture with citric acid in a powder form, and grinding to obtain a gel mixture; according to the preparation method, the preparation process of the transition metal oxide positive electrode active material can be simplified to a certain extent, the preparation efficiency of the transition metal oxide positive electrode active material is improved, and the preparation cost of the transition metal oxide positive electrode active material is reduced.

Polyethyleneglycol modificatory tecombined human granulocyte colony stimulating factor mutant

The invention relates to a preparation method and a validation method of a polyethyleneglycol modificatory recombined human granulocyte colony stimulating factor mutant (PEG-rmhG-CSF). Cysteine (cys) at the 18th site of the rhG-CSF is mutated into serine (ser) through a point mutation technology, then the rhG-CSF (wherein the 18-cysteine is mutated into the serine) which is mutated at the N end is expressed by a phage display technology, and then the rmhG-CSF with high stability and biologic activity is selected by utilizing a receptor of the rhG-CSF. The mutated rmhG-CSF reacts with polyethyleneglycol (PEG) with an amido reactive group, thus the PEG-rmhG-CSF with higher activity and stability is obtained after separation and purification.

Modification method of positive electrode material, positive electrode material, battery positive electrode, battery electrode assembly, sodium ion battery and electric equipment

The invention provides a modification method of a positive electrode material, the positive electrode material, a battery positive electrode, a battery electrode assembly, a sodium ion battery and electric equipment, and belongs to the field of new material preparation and energy storage. According to the modification method of the positive electrode material, the sodium-ion battery positive electrode material and boric acid are sequentially mixed and sintered, and through the modification method, the stability of the battery positive electrode material in air can be improved to a certain extent, so that the electrical property of the battery positive electrode material is ensured.

Broken root bag penetrating prevention treatment method for leucaena leucocephala cv. Salvador nursery-grown plants

The invention relates to a broken root bag penetrating prevention treatment method for leucaena leucocephala cv. Salvador nursery-grown plants, and belongs to the technical field of afforestation. The treatment method relates to nursery-grown plant treatment requirements and a treatment technology and specifically includes the steps that annual container-grown seedlings are adopted in bag penetrating prevention treatment, containers are generally nutrition bags with the specifications being 11 cm*14 cm, the bag seedlings are pulled up by the roots 10-20 days before the nursery-grown plants are planted, one thing to note is that the nutrition bags cannot be broken when the seedlings are pulled up, and soil balls in the nutrition bags cannot be broken either; the root systems of the nursery-grown plants which penetrate out of the nutrition bags are cut, trunks of 0.6-1.5 m are reserved, and the redundant parts are cut off; lateral branches and leaves are removed, seedling ridge furrows are ...

神经营养因子的生产方法

... 本发明公开了一种神经营养因子的生产方法。它通过计算机辅助设计，对人睫状神经营养因子的基因进行修饰改造，获得改造后突变人睫状神经营养因子基因的cDNA，人工合成该cDNA，构建重组质粒，转化大肠杆菌，经全自动发酵罐高密度表达；以切向流超滤方式进行复性；以离子交换层析、疏水层析和分子筛层析顺序组合纯化，生产得到高纯度的睫状神经营养因子。本发明的优点：1)采用计算机辅助设计，对天然密码子进行了优化，实现了突变体在大肠杆菌中的稳定高表达。2)采用切向流超滤方式复性，可减少超滤过程中的极化现象。3)离子交换层析、疏水层析和分子筛层析三种不同分离机理层析顺序组合，使复性后的CNTF得以纯化精制，其纯度均大于95％。 ...

Method for production of recombining human grunulocyte colony stimulation factor

The present invention relates to a production method of recombined human granulocyte colony stimulating factor (rhG-CSF). Said method includes the following steps: adopting polyase chain reaction after reverse transcription (TR-PCR)l hook-taking human granulocyte colony stimulating factor gene; transforming E. coli; renaturation of protein in the mode of hollow fiber ultrafiltration dialysis; making the above-mentioned material pass through such processes of ion exchange chromatography, hydrophobic chromatography and molecular sieve chromatography which are combined together sequentally; purifying so as to obtain a lot of high-purity medicinal rhG-CSF protein.

Polyglycol single-modificatory recombined human granular leukocyte colony stimulating factor mutant and preparation method thereof

The invention discloses a high activity polyethylene glycol single-modified recombinant human granulocyte colony stimulating factor mutant (PEG-rmhG-CSF), the preparation method and the medical application thereof. A free cysteine (Cys) of the recombinant human granulocyte colony stimulating factor mutant (rmhG-CSF) is subject to the site-directed chemical modification with a polyethylene glycol sulfhydryl reaction reagent to obtain a PEG-rmhG-CSF product with higher activity and more homogeneous structure. The product can be used for the treatment of neutrophilic granulocytopenia caused by radiotherapy and chemotherapy, etc.

Funnel bottom fish-scale pit soil preparation technology

The invention relates to funnel bottom fish-scale pit soil preparation technology and belongs to the technical field of afforestation soil preparation. The funnel bottom fish-scale pit soil preparation technology is characterized in that semilunar pits are dug on a steep barren mountain slope from top to bottom along a contour line and arranged in the shape of a Chinese character 'pin', and a vertical side of each semilunar pit faces the upper portion of the barren mountain slope while a semilunar face of the same faces the lower portion of the barren mountain slope; one side of each semilunar is the vertical side, the side, close to the vertical side, of each semilunar is semilunar, each semilunar pit is a funnel bottom with a large upper opening and a small lower opening, is fish-scale-shaped and is called a funnel bottom fish-scale pit, soil preparation specification of each semilunar pit is width of the upper opening x longitudinal width x depth=100cmx80cmx60cm, and specific specification ...

Information recommendation method and system

The invention provides an information recommendation method and system. The information recommendation method includes the steps that net friend comments related to one or more keywords of current information are acquired; text clustering is conducted on the acquired net friend comments, one or more focus points contained in the acquired net friend comments and one or more pieces of emotion or opinion expression information for the one or more focus points are found out; based on an emotion or opinion expression corpus, emotion or opinion tendency information for the one or more focus points in the acquired net friend comments is acquired through the one or more pieces of emotion or opinion expression information for the one or more focus points in the acquired net friend comments, and the emotion or opinion tendency information is displayed. By the adoption of the information recommendation method and system, emotion or opinion tendency information to which a network user pays attention ...

Sandwich insulating plate manufacturing method

The invention discloses a method for manufacturing a sandwich insulating plate, which belongs to the technical field of shaft insulation of a hydro-generator, and is characterized by comprising the following steps of: a, cutting a cold-roll steel plate into a fan-shaped steel plate, and slotting and knurling the surface of the fan-shaped steel plate; b, insulating paper is placed between the two fan-shaped steel plates, and a sandwich insulating plate is formed through high-temperature bonding and pressing; c, grinding the two surfaces of the sandwich insulating plate until the flatness reaches the standard; d, performing water cutting on the sandwich insulating plate; e, baking the sandwich insulating plate at low temperature, and measuring the dimensional accuracy and the insulation resistance of the sandwich insulating plate; and f, according to the data of the sandwich insulating plate, carrying out local clamp repair, packaging and carrying out moisture-proof treatment on the exposed ...

Method for production of recombining human grunlocyte colony stimulation factor

Method for preparing solid acid esterification catalyst by utilizing zinc smelting water quenching slag

The invention provides a method for preparing SO4<2-> or -SO3H (sulfonic group)/water quenching slag solid acid by utilizing zinc-containing wastes. The method comprises the following steps: carrying out wet impregnation to remove sulfions from water quenching slag, and processing the zinc smelting water quenching slag with 1-40% of sulfuric acid or sulfonic acid until no gas is released; drying the obtained separated jelly-like sample at 30-120DEG C for 0.5-8h, calcining the sample at 120-350DEG C for 1-10h, putting the calcined sample in a mortar, and grinding to powder with the diameter of 0.001-2cm; adding 1-30mass% of concentrated sulfuric acid or sulfonic acid into the sample, carrying out dry ageing at 20-80DEG C for 1-24h; and drying the aged sample at 30-120DEG C for 0.5-8h, and calcining the dried sample at 300-650DEG C for 1-10h to prepare the SO4<2-> or -SO3H/water quenching slag solid acid.