28-07-2023 дата публикации
Номер: CN116497032A
Принадлежит:
The invention provides an optimization method of a 5 'UTR (Untranslated Region) sequence, an optimized 5' UTR sequence and application. The optimization method of the 5 'UTR sequence for improving the ribosome load comprises the steps that part of original sequences in the 5' UTR sequence are replaced with optimization sequences, and the optimization sequences comprise a first motif with the length being 5 basic groups, a second motif with the length being 7 basic groups or a third motif with the length being 9 basic groups; the motif center of the optimized sequence is adenine or uracil, and the motif center is a basic group position in the middle of a character string of the optimized sequence; the optimization method using the first, second or third motifs comprises the step of replacing an original sequence composed of the-44-40 < th > position of the 5'end of the initiation codon AUG of mRNA with the first motif, the second motif or the third motifs, the problem that in the prior art ...
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