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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 14. Отображено 14.
01-02-2012 дата публикации

Bacillus subtilis lipase, and preparation method and application thereof

Номер: CN0101857856B
Автор: CUI ZONGQIANG, WANG DIANBING, WANG XIAOYING, WEI HONGPING, ZHANG XIANEN, ZHANG ZHIPING, ZHOU YAFENG, ZONGQIANG CUI, DIANBING WANG, XIAOYING WANG, HONGPING WEI, XIANEN ZHANG, ZHIPING ZHANG, YAFENG ZHOU
Принадлежит:

The invention discloses a bacillus subtilis lipase, and a preparation method and application thereof. A bacillus subtilis lipase and an Escherichia coli BL21/pET28a-/Lip A f10-H1 (I12V/L140F/D144E), CCTCC NO: M2010058. The mutant coding gene of the high-activity lipase has nucleotide sequence indicated by SEQ ID NO.1and the encoded protein thereof has amino acid sequence indicated by SEQ ID NO.2.The method comprises the following steps, firstly, bacillus subtilis lipase Lip A structural gene is amplified through PCR, secondly, three pairs of mutagenic primers are designed and the mutant is introduced into the lipase Lip A gene by superposing the PCR, thirdly, high-activity lipase generation bacteria BL21 (DE3)/Lip A(I12V/L140F/D144E) is obtained, fourthly, target protein is induced and expressed in the Escherichia coli, and fifthly, the protein is purified. The invention further relates to the application of the bacillus subtilis lipase in detergent industry. The invention has easy operation ...

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Номер записи: 1
27-03-2018 дата публикации

Tightness test frock of pipeline part

Номер: CN0207147712U
Автор: GENG CHUANXING, YOU CHAO, CHEN BAOLIAN, WANG DIANBING, FANG TAO, CUI LEI, JIA JUN
Принадлежит: CHINA MERCHANTS HEAVY IND JIANGSU CO LTD, CHINA MERCHANTS HEAVY IND SHENZHEN CO LTD, CHINA MERCHANTS HEAVY INDUSTRY (JIANGSU) CO., LTD., CHINA MERCHANTS HEAVY INDUSTRY (SHENZHEN) CO., LTD.

The utility model discloses a tightness test frock of pipeline part, including first gland and the second gland that sets up side by side, both connect through a plurality of fasteners, one side thatfirst gland is close to the second gland is equipped with first sleeve pipe, the outside that first sleeve pipe is close to the one end of second gland is equipped with the second sleeve pipe, secondsleeve pipe and second gland fixed connection, the central authorities of second gland are equipped with the through -hole, be equipped with sealed rubber ring between second sleeve pipe and the firstsleeve pipe, the central authorities of first gland are equipped with the connecting pipe that communicates with first sleeve pipe, the inboard of connecting pipe outer end is equipped with the internal thread. This frock can make the pipeline part can all accomplish intensity tightness test in the workshop, can intactly carry out surface paint after experimental to handle, simultaneously on theship ...

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Номер записи: 2
09-05-2023 дата публикации

Multifunctional efficient machine tool production line

Номер: CN116079501A
Автор: WANG DIANBING, MAO PEIPEI
Принадлежит:

The multifunctional efficient machine tool production line comprises at least one machining center set which is connected with each other, and each machining center set comprises a first machining center and a second machining center which are connected with each other; the first machining center comprises a first base and a first stand column, a first installation frame is transversely installed on the first stand column in a sliding mode, a first machine head is vertically installed on the first installation frame in a sliding mode, a first clamping chuck is installed on the first machine head, and a first power driving part is connected to the first clamping chuck. A first numerical control tool turret is longitudinally and slidably mounted on the first base; the second machining center comprises a second base and a second stand column, a second mounting frame is transversely and slidably mounted on the second stand column, a second machine head is vertically and slidably mounted on ...

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Номер записи: 3
22-09-2010 дата публикации

Bacillus subtilis lipase, preparation method and application

Номер: CN0101838634A
Автор: ZONGQIANG CUI, DIANBING WANG, XIAOYING WANG, HONGPING WEI, XIANEN ZHANG, ZHIPING ZHANG, YAFENG ZHOU, CUI ZONGQIANG, WANG DIANBING, WANG XIAOYING, WEI HONGPING, ZHANG XIANEN, ZHANG ZHIPING, ZHOU YAFENG
Принадлежит:

The invention discloses bacillus subtilis lipase, a preparation method and application. The bacillus subtilis lipase is expressed, on Escherichia coli BL21/Pet28a-Lip A f12-C2 (R33W/L140F), as CCTCC NO: M2010057. Coding genes of a high-activity lipase mutant have a nucleotide sequence shown as SEQ ID NO.1, and proteins coded by the coding genes have an amino acid sequence shown as SEQ ID NO.2. The preparation method comprises the following steps of: 1, performing polymerase chain reaction (PCR) amplification to generate bacillus subtilis Lip A gene; 2, designing two pairs of mutagenic primers and sequentially introducing two mutational sites into the Lip A genes by using an overlapped PCR method; 3, obtaining high-activity lipase producing bacteria BL21 (DE3)/Lip A (R33W/L140F); 4, performing inducible expression on target proteins in the Escherichia coli BL21 (DE3); and 5, purifying the proteins. The invention also discloses the application of the bacillus subtilis lipase in leatherworking ...

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Номер записи: 4
02-11-2011 дата публикации

Kit and method for detecting influenza A virus based on immune magnetic bead enrichment

Номер: CN0102230938A
Автор: XIANEN ZHANG, DIANBING WANG, HONGPING WEI, ZHIPING ZHANG, ZHANG XIANEN, WANG DIANBING, WEI HONGPING, ZHANG ZHIPING
Принадлежит:

The invention discloses a kit and a method for detecting influenza A virus based on immune magnetic bead enrichment. The kit comprises A, an immune enrichment reaction system component and B, a loop-mediated isothermal nucleic acid amplification system component; and the preparation method of the components of the kit comprises C, preparing an antibody, D, preparing immune magnetic beads and E, designing a specific primer of influenza A virus subtype H1N1 for loop-mediated isothermal amplification reaction at 65 DEG C. The detection method comprises the following steps of: a, performing immune enrichment; b, thermally cracking a product, separating through a magnetic frame and taking supernate containing virus nucleic acid as a template for the loop-medicated isothermal amplification reaction; c, performing the loop-medicated isothermal amplification reaction; and d, determining a result. The kit has the advantages of high specificity, high sensitivity, low hardware requirement and the ...

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Номер записи: 5
11-03-2015 дата публикации

Quick changing device for partition board used for achieving dual stations of transverse cutting unit

Номер: CN104400112A
Автор: WANG DIANBING, HE JINGHAO
Принадлежит:

The invention relates to a quick changing device for a partition board used for achieving dual stations of a transverse cutting unit, and belongs to the technical field of steel product machining devices. The quick changing device is characterized by comprising a partition board base vertically mounted on a side of a piling bed and a quick-changing partition board mounted on the partition board base in pluggable manner; the quick-changing partition board is designed to be of a T-shaped structure; and the partition board base is provided with a T-shaped groove and a sealing positioning end, wherein the T-shaped groove is used for accepting the quick-changing partition board and is matched with the quick-changing partition board in shape, and the sealing positioning end is located on the lower portion of the T-shaped groove and positions the quick-changing partition board. The quick changing device is simple and practical in integral structural design, and the working efficiency is improved ...

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Номер записи: 6
21-09-2011 дата публикации

Gene encoding of firefly luciferase, its preparation method and application

Номер: CN0102191213A
Автор: HONGPING WEI, JINFENG ZHAO, DIANBING WANG, ZHIPING ZHANG, XIANEN ZHANG, WEI HONGPING, ZHAO JINFENG, WANG DIANBING, ZHANG ZHIPING, ZHANG XIANEN
Принадлежит:

The invention relates to a gene encoding of firefly luciferase, its preparation method and application, which comprises the following steps: 1. introducing mutation sites of mutant enzymes; 2. identifying recombinant plasmids; 3. sequencing to obtain polymerase chain reaction (PCR) fragment with 1060 mutation sites; 4. obtaining escherichia by using restriction endonucleasecoli purifying after recovering and purifying through gel; 5. identifying recombinant plasmids to obtain a separating protein gene; 6. culturing escherichia coli, 7. converting recombinant plasmids into BL21 competent cell to obtain bacterial strain capable of expressing mutant enzymes, 8. culturing the obtained BL21 expression bacterial strain; 9. centrifuging bacteria liquid of protein, centrifuging to obtain supernatant through ultrasonic fragmentation; 10. determining to obtain firefly luciferase with high heating stability and high enzyme activity. The invention relates to application of firefly luciferase gene on ...

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Номер записи: 7
25-04-2012 дата публикации

Bacillus subtilis lipase, preparation method and application

Номер: CN0101838634B
Автор: CUI ZONGQIANG, WANG DIANBING, WANG XIAOYING, WEI HONGPING, ZHANG XIANEN, ZHANG ZHIPING, ZHOU YAFENG, ZONGQIANG CUI, DIANBING WANG, XIAOYING WANG, HONGPING WEI, XIANEN ZHANG, ZHIPING ZHANG, YAFENG ZHOU
Принадлежит:

The invention discloses bacillus subtilis lipase, a preparation method and application. The bacillus subtilis lipase is expressed, on Escherichia coli BL21/Pet28a-Lip A f12-C2 (R33W/L140F), as CCTCC NO: M2010057. Coding genes of a high-activity lipase mutant have a nucleotide sequence shown as SEQ ID NO.1, and proteins coded by the coding genes have an amino acid sequence shown as SEQ ID NO.2. The preparation method comprises the following steps of: 1, performing polymerase chain reaction (PCR) amplification to generate bacillus subtilis Lip A gene; 2, designing two pairs of mutagenic primers and sequentially introducing two mutational sites into the Lip A genes by using an overlapped PCR method; 3, obtaining high-activity lipase producing bacteria BL21 (DE3)/Lip A (R33W/L140F); 4, performing inducible expression on target proteins in the Escherichia coli BL21 (DE3); and 5, purifying the proteins. The invention also discloses the application of the bacillus subtilis lipase in leatherworking ...

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Номер записи: 8
13-10-2010 дата публикации

Bacillus subtilis lipase, and preparation method and application thereof

Номер: CN0101857856A
Автор: ZONGQIANG CUI, DIANBING WANG, XIAOYING WANG, HONGPING WEI, XIANEN ZHANG, ZHIPING ZHANG, YAFENG ZHOU, CUI ZONGQIANG, WANG DIANBING, WANG XIAOYING, WEI HONGPING, ZHANG XIANEN, ZHANG ZHIPING, ZHOU YAFENG
Принадлежит:

The invention discloses a bacillus subtilis lipase, and a preparation method and application thereof. A bacillus subtilis lipase and an Escherichia coli BL21/pET28a-/Lip A f10-H1 (I12V/L140F/D144E), CCTCC NO: M2010058. The mutant coding gene of the high-activity lipase has nucleotide sequence indicated by SEQ ID NO.1and the encoded protein thereof has amino acid sequence indicated by SEQ ID NO.2. The method comprises the following steps, firstly, bacillus subtilis lipase Lip A structural gene is amplified through PCR, secondly, three pairs of mutagenic primers are designed and the mutant is introduced into the lipase Lip A gene by superposing the PCR, thirdly, high-activity lipase generation bacteria BL21 (DE3)/Lip A(I12V/L140F/D144E) is obtained, fourthly, target protein is induced and expressed in the Escherichia coli, and fifthly, the protein is purified. The invention further relates to the application of the bacillus subtilis lipase in detergent industry. The invention has easy operation ...

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Номер записи: 9
21-01-2015 дата публикации

Device capable of quickly fixing travelling crane lifting hook

Номер: CN104291211A
Автор: HE JINGHAO, WANG DIANBING
Принадлежит:

The invention provides a device capable of quickly fixing a travelling crane lifting hook and overcomes the technical problem that the current device capable of quickly fixing the travelling crane lifting hook is time-consuming and labor-consuming in disassembly and assembly. The device capable of quickly fixing the travelling crane lifting hook comprises a U-shaped plate, two vertical plates and a semicircular annular plate, wherein the lower ends of the two vertical plates are fixedly connected with the U-shaped plate respectively; the two vertical plates are respectively perpendicular to the plane of the U-shaped plate; the upper ends of the two vertical plates are fixedly connected with two ends of the semicircular annular plate; a second semicircular hole matched with a first semicircular hole of the semicircular annular plate is formed in the middle part of the upper side of each vertical plate; the first semicircular holes and the second semicircular holes are combined to form a ...

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Номер записи: 10
24-06-2015 дата публикации

High-activity methyl parathion hydrolase mutant and use thereof

Номер: CN104726426A
Автор: ZHENG CHUNYANG, WANG LEI, BAO PENG, ZHANG XIAN'EN, ZHANG ZHIPING, WANG DIANBING, WANG LIN
Принадлежит:

The invention discloses high-activity methyl parathion hydrolase and a use thereof. Compared with the existing methyl parathion hydrolase, the recombinant methyl parathion hydrolase obtained by site directed mutagenesis design and screening has substantially improved activity, and has a good capability of degrading organophosphorus pesticide such as methyl parathion. A fruit and vegetable pesticide cleaning agent prepared from the methyl parathion hydrolase has effects better than those of the same kind products on the market. The high-activity methyl parathion hydrolase can be used for removing pesticide on fruits and vegetables, tea and traditional Chinese medicine raw materials in processing, can be used in soil restoration and has a wide application prospect. The invention also discloses a preparation method of the recombinant methyl parathion hydrolase.

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Номер записи: 11
21-05-2014 дата публикации

Novel multifunctional nano biomaterial based on S-layer protein in-vitro self-assembly

Номер: CN103805580A
Автор: ZHANG XIANEN, WANG DIANBING, WANG XUYING, ZHANG ZHIPING
Принадлежит:

The invention discloses a brand-new novel multifunctional nano biomaterial based on S-layer protein in-vitro self-assembly, which is prepared through fusion expression of bacillus anthracis S-layer protein EA1 and methyl parathion hydrolase (MPH) for the first time on the basis of an in-vitro self-assembly technology. The material preparation comprises the steps of construction of recombinant vectors PQE3-MPH and PQE30-EA1-MPH, expression and purification of fusion protein, and in-vitro self-assembly of the fusion protein. The biomaterial disclosed by the invention is obvious in biological function, and is capable of prolonging MPH storage time, completely taking effect for a long term in organophosphorus pesticide degradation and reducing cost; and the biomaterial has an application prospect in bacterium inhibition and sterilization, and is capable of remarkably improving detection sensitivity and applicable to development of high-sensitivity detecting elements.

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Номер записи: 12
17-06-2015 дата публикации

PEG modified methyl parathion hydrolase (MPH) and application thereof

Номер: CN104711239A
Автор: ZHENG CHUNYANG, XIAO GUIPENG, ZHANG XIAN'EN, ZHANG ZHIPING, WANG DIANBING, WANG LIN
Принадлежит:

The invention discloses a PEG modified methyl parathion hydrolase (MPH) and an application thereof as a pesticide degrading agent. The preparation method of PEG modified MPH mainly comprises the following steps: placing MPH in a proper buffer liquid system, adjusting the pH value, adding a PEG modifier, carrying out modification reactions at a constant temperature of 4 to 37 DEG C, and performing combination according to the activity and stability characteristics of products with different modification degrees under the assist of different purification methods. After combination, the PEG modified MPH can be used as a pesticide degrading agent, the activity and stability are controllable, and the cost is reduced. The PEG modified MPH can be made into concentrate additive, disintegrating tablet, powder, and the like, is used to eliminate residual pesticide on the surface of fruits and vegetables, can also be used to remove residual pesticide during the processing process of agricultural products ...

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Номер записи: 13
07-09-2011 дата публикации

Detection method of mycobacterium tuberculosis pyrazinamide drug resistance

Номер: CN0102174652A
Автор: HONGPING WEI, MAN ZHOU, DIANBING WANG, ZHIPING ZHANG, XIANEN ZHANG, WEI HONGPING, ZHOU MAN, WANG DIANBING, ZHANG ZHIPING, ZHANG XIANEN
Принадлежит:

The invention discloses a detection method of mycobacterium tuberculosis pyrazinamide drug resistance, comprising the following steps: A. pyrolyzing a template of mycobacterium tuberculosis used for polymerase chain reaction (PCR); B. designing a PCR primer, and augmenting a full-length pyrazinamide (pncA) gene; C. concentrating the augmented pncA gene segment and quantifying; D. expressing pncA enzyme by adopting a wheat germ acellular expression system; and F. determining the expressed pncA enzymatic conversion pyrazinamide into the activity of pyrazine acid; comparing the pncA enzymatic activities of a sensitive strain and a drug resistance strain of the expressed tubercle bacillus standard; and determining the drug resistance of the mycobacterium tuberculosis. The method is characterized in that the drug resistance caused by pncA gene mutation can be determined by a pair of primers, and simultaneously discloses a primer sequence for augmenting the full-length pncA gene from the tubercle ...

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Номер записи: 14