23-05-2013 дата публикации
Номер: US20130129764A1
The invention relates to compositions and methods for the detection of various infectious organisms, including heartworm (), , and . More particularly, this invention relates to antibodies that bind to a heartworm antigen, the gp36 polypeptide, the p44 polypeptide, the OspA, OspC, OspF, p39, p41 and VlsE polypeptides, and uses thereof. 1Anaplasma phagocytophilumA. phagocytophilumA. phagocytophilum. An p44 polypeptide comprising amino acids 222-236 of SEQ ID NO:1 , wherein said polypeptide comprises at least one mutation , an p44 polypeptide comprising amino acids 222-237 , 222-238 , 222-239 , 222-240 , 222-241 , 222-242 , 222-243 , 222-244 , 222-245 , 222-246 , or 222-247 of SEQ ID NO:1 , or an p44 polypeptide comprising amino acids 222-237 , 222-238 , 222-239 , 222-240 , 222-241 , 222-242 , 222-243 , 222-244 , 222-245 , 222-246 , or 222-247 of SEQ ID NO:1 that comprises at least one mutation.2. A polypeptide comprising a multimer claim 1 , a combination claim 1 , or a chimera of the polypeptides of .3Anaplasma phagocytophilum. An p44 polypeptide that exhibits at least 75% identity to the amino acid sequence of SEQ ID NO:1 claim 1 , or the amino acids 222-237 claim 1 , 222-238 claim 1 , 222-239 claim 1 , 222-240 claim 1 , 222-241 claim 1 , 222-242 claim 1 , 222-243 claim 1 , 222-244 claim 1 , 222-245 claim 1 , 222-246 claim 1 , or 222-247 of SEQ ID NO:1 claim 1 , wherein said polypeptide is not a wild-type P44 protein claim 1 , and wherein said polypeptide binds to an antibody that is specific for a wild-type P44 protein.4Anaplasma phagocytophilumA. phagocytophilum. A polynucleotide which encodes an p44 polypeptide comprising the amino acid sequence of SEQ ID NO:1 claim 1 , or a complimentary strand thereof claim 1 , wherein said polynucleotide is not a wild-type P44 polynucleotide claim 1 , or a polynucleotide which encodes an p44 polypeptide having the amino acid sequence of 222-237 claim 1 , 222-238 claim 1 , 222-239 claim 1 , 222-240 claim 1 , 222-241 claim 1 , ...
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