Negative selection of cell disease targets

The invention relates to negative selection of cell disease targets, in particular to the use of negative screening measurement based on RNAi to identify genes involved in activation of regulatory elements.

HIV medicament screening cell model and special pseudotype lentivirus therefor

The invention discloses an HIV drug screening cell model and special pseudotype slow virus thereof; the invention constructs recombinant plasmid for expressing Gag gene and Pol gene of HIV, recombinant slow-virus plasmid for expressing reporter gene and recombinant plasmid for expressing Rev gene of HIV; the three plasmids and recombinant plasmid for expressing glycoprotein gene of capsid G of herpetic stomatitis are transfected in the cells of mammals so as to obtain pseudotype slow virus; the pseudotype slow virus is used for infecting the cells of mammals, thus obtaining the HIV drug screening cell model based on the reporter gene. The HIV drug screening cell model provided by the invention uses the virus with one-time infection with good safety and the reporter gene leads the cell model to have super high sensitivity.

Combustion apparatus for EGR valve test

The invention discloses a combusting device used for an EGR valve test, which comprises a blower fan, an air cleaner, a first throttle valve, a second throttle valve, a combustor, an oil box, an oil pump, a fuel oil filter, an igniter, a first air inlet, a fuel oil spraying nozzle, a sparking plug and a second air inlet, wherein, the blower fan is respectively connected with the first throttle valve and the second throttle valve through the air cleaner, the first throttle valve is connected with the first air inlet of the combustor, the second throttle valve is mutually connected with the second air inlet of the combustor, the oil box is connected with the oil pump, the fuel oil filter and the fuel oil spraying nozzle in sequence, and the igniter is connected with the sparking plug. The combusting device can heat the inlet air by adopting a combusting and heat emitting method, to cause the oil and the air to be sufficiently mixed; both the air flow quantity and the air fuel ratio can be ...

Two stage hot pipe EGR cooler

The invention discloses a two-stage thermotube type EGR cooler. The two-stage thermotube type EGR cooler mainly consists of a shell with an exhaust gas inlet and an exhaust gas outlet respectively arranged on two ends of the cooler and a two-stage thermotube with heating sections arranged inside the shell. The thermotube is communicated with four pipe sections of a heating section, a steam tube, a condensation section and a condensation tube in turn from beginning to end; the heating sections of the two-stage thermotube are arranged along the exhaust gas flow direction in order; and the condensation sections of the two-stage thermotube are overlapped and arranged along the radiation wind blowing direction. In the two-stage thermotube, a first stage thermotube and a second stage thermotube respectively adopt water and methanol as cooling mediums. The two-stage thermotube type EGR cooler adopts the principle of cooling exhaust gas by the thermotube technology and has good cooling effect.

Combustion apparatus for EGR valve test

The invention discloses a combusting device used for an EGR valve test, which comprises a blower fan, an air cleaner, a first throttle valve, a second throttle valve, a combustor, an oil box, an oil pump, a fuel oil filter, an igniter, a first air inlet, a fuel oil spraying nozzle, a sparking plug and a second air inlet, wherein, the blower fan is respectively connected with the first throttle valve and the second throttle valve through the air cleaner, the first throttle valve is connected with the first air inlet of the combustor, the second throttle valve is mutually connected with the second air inlet of the combustor, the oil box is connected with the oil pump, the fuel oil filter and the fuel oil spraying nozzle in sequence, and the igniter is connected with the sparking plug. The combusting device can heat the inlet air by adopting a combusting and heat emitting method, to cause the oil and the air to be sufficiently mixed; both the air flow quantity and the air fuel ratio can be ...

Continuous rotary valve for EGR valve hot gas periodical impact fatigue durability test

Two stage hot pipe EGR cooler

The invention discloses a two-stage thermotube type EGR cooler. The two-stage thermotube type EGR cooler mainly consists of a shell with an exhaust gas inlet and an exhaust gas outlet respectively arranged on two ends of the cooler and a two-stage thermotube with heating sections arranged inside the shell. The thermotube is communicated with four pipe sections of a heating section, a steam tube, a condensation section and a condensation tube in turn from beginning to end; the heating sections of the two-stage thermotube are arranged along the exhaust gas flow direction in order; and the condensation sections of the two-stage thermotube are overlapped and arranged along the radiation wind blowing direction. In the two-stage thermotube, a first stage thermotube and a second stage thermotuberespectively adopt water and methanol as cooling mediums. The two-stage thermotube type EGR cooler adopts the principle of cooling exhaust gas by the thermotube technology and has good cooling effect. In ...

Exhaust gas recirculation valve and cooler combined test device

The invention discloses a test device for combining an exhaust gas recirculation valve with a cooler. Through a first three-way valve, a second three-way valve and a first four-way valve, a high-temperature gas device not only can be singly connected with a recirculation valve for exhaust gas to be tested, but also can be separately connected with a recirculation cooler for the exhaust gas to be tested, or can be jointly connected with the recirculation valve for the exhaust gas to be tested and the recirculation cooler for the exhaust gas to be tested; then through a second four-way valve and a fourth three-way valve, a high-temperature gas device is connected to a flow meter for measuring the flow. Meanwhile, two additional switch valves are installed in a pipeline, so as to control theprecedence sequence during the combination measurement of the recirculation valve for the exhaust gas to be tested and the recirculation cooler for the exhaust gas to be tested. The test device can be used ...

HIV medicament screening cell model and special pseudotype lentivirus therefor

The invention discloses an HIV drug screening cell model and special pseudotype slow virus thereof; the invention constructs recombinant plasmid for expressing Gag gene and Pol gene of HIV, recombinant slow-virus plasmid for expressing reporter gene and recombinant plasmid for expressing Rev gene of HIV; the three plasmids and recombinant plasmid for expressing glycoprotein gene of capsid G of herpetic stomatitis are transfected in the cells of mammals so as to obtain pseudotype slow virus; the pseudotype slow virus is used for infecting the cells of mammals, thus obtaining the HIV drug screening cell model based on the reporter gene. The HIV drug screening cell model provided by the invention uses the virus with one-time infection with good safety and the reporter gene leads the cell model to have super high sensitivity.

FUSION PROTEINS FOR IMAGING THE NUCLEUS AND CHROMOSOMES OF LIVE CELLS

Fusion proteins that fluoresce and bind DNA, as well as nucleic acid constructs encoding the same are described herein. These imaging agents function in live cells to bind DNA and are thus useful for visualizing nuclei and chromosomes to make observations of nuclear and chromosomal structure and dynamics. The imaging agents may be used in screening assays to test the activity of biological effector molecules.

Hot pipe EGR cooler

The invention discloses a heat pipe EGR cooler, mainly comprising a shell and a heat pipe, wherein, two ends of the shell are provided with a waste gas inlet and a waste gas outlet respectively; and the heat pipe consists of a heating section, a steam pipe, a condensing section and a condensation pipe which are communicated from beginning to end. The invention is characterized in that the heating section is arranged in the shell. The principle of the invention is to adopt the heat pipe technology to cool waste gas, which can ensure that the temperature of the waste gas is lower and can effectively relieve the influence of the waste gas to the power per liter of the engine on the premise of the same recirculation waste gas amount. Meanwhile, the heat pipe EGR cooler can effectively reduce the negative effect of the reduced heat exchanging efficiency caused by caking, and can effectively avoid a leaking occasion of welding lines of flange faces between the shell and the waste gas inlet and ...

Expression vector for constructing high-quality bacteriophage antibody library

The invention discloses an expression vector for constructing a high-quality bacteriophage antibody library. The vector disclosed by the invention is prepared by the following steps: 1) inserting coding genes of a light-chain region SacB in multiple cloning sites of pDF to obtain an intermediate vector PSBX; 2) mutating BssHII enzyme cutting sites of the intermediate vector PSBX obtained in the step 1) into BglII enzyme cutting sites to obtain an intermediate vector PSGX; and 3) inserting coding genes of a heavy-chain region SacB in Nhe I and Nco I enzyme cutting sites of the intermediate vector PSGX obtained in the step 2) to obtain a vector pDF-D-SacB. Experiment disclosed by the invention proves that the obtained phagemid vector pDF-D-SacB containing suicide genes is applicable to the construction of a high-capacity bacteriophage antibody library.

HIV stain drug-resistant phenotype analytical cell model and special pseudotype lentivirus therefor

The invention discloses a cell model of HIV strain phenotypic drug resistance analysis and special pseudotype slow virus thereof; the invention constructs plasmid for expressing Gag protein of HIV, recombinant slow-virus plasmid for expressing reporter gene and helper plasmid for expressing Rev protein of HIV; under the effect of the helper plasmid pVSV-G, the three plasmid and HIV reverse transcriptase and the gene fragment of protease which are amplified from strain can obtain the pseudotype slow virus of the HIV reverse transcriptase and protease gene containing strain. The pseudotype slow virus is used for infecting the cells of mammals, thus obtaining the strain phenotypic resistance cell model based on the reporter gene. The cell model of the invention can carry out phenotypic resistance analysis to the strain, the reporter gene leads the cell model to have super high sensitivity, and the cell model of HIV strain phenotypic drug resistance analysis is applicable to the phenotypic ...

Exhaust recycled valve multi-functional test device

The invention discloses a waste-gas recycling valve multi-function test apparatus. The gas outlet of a burner is successively connected with a cooler, a continuous rotary valve, one end of an overflow valve and the inlet of a waste-gas recycling valve under test; the gas inlet of a vacuum pump is successively connected with one end of the overflow valve, a two-position three-way valve and a vacuum tube of the waste-gas recycling valve under test; the outlet of the waste-gas recycling valve under test is successively connected with an exhaust cooler, a counterbalance valve, a high-temperature two-position three-way valve and a mass flowmeter, and then is filled with atmosphere; the water inlet of the cooler is successively connected with a speed-adjustable water pump and a cooling water tank; and the water outlet of the cooler is connected with a drain pipe. The invention can be used to test the flux characteristics of a waste-gas recycling pump, the dynamic characteristics of a vacuum waste-gas ...

Fusion protein for imaging nucleus and chromosomes in living cell

The invention provides a fusion protein for imaging a nucleus and chromosomes in a living cell. The invention also provides an imaging agent containing the fusion protein, nucleic acid for coding theimaging agent, a nucleic acid constructor and a carrier containing the nucleic acid, a cell containing the imaging agent, the nucleic acid, the nucleic acid constructor or the carrier and a fit containing one of the imaging agent, the nucleic acid, the nucleic acid constructor or the carrier. The imaging agent can be used for imaging the nucleus and the chromosomes of the cell, monitoring relevantchanges of the nucleus in the cell and testing the activity of a biological effect molecule.

Core of shell-and-tube heat exchanger and connection method of tube plate and tube

The invention discloses a core of a shell-and-tube heat exchanger and a connection method of a tube plate and a tube. In the design, flanging on a tube plate hole is added on the basis of the original structure. The design scheme increases braze welding joint area with the tube, and enhances strength of a welding line, thus leading the braze welding line not to crack easily. The adoption of the compound tube plate form enhances strength of the root part of the tube, and reduces stress at the connection part of the tube and the tube plate, thus leading the tube to bear the mechanical stress and the thermal stress of the use environment to a greater degree, and leading the tube not to crack easily; therefore, the technical scheme effectively solves shortcomings in the original scheme, and is more suitable for the requirement of use reliability.

Continuous rotary valve for EGR valve hot gas periodical impact fatigue durability test

The invention discloses a continuous rotary valve used for an EGR valve fatigue durability test under periodic impaction of hot gas. A connecting pipe, grooves and through holes are respectively arranged at both sides of a casing body, a rotator is arranged inside the casing body, a through hole and the grooves are arranged on the rotator, a gear is arranged at one end of the rotator, a left supporting plate, a washer and a sealing cover used for positioning the casing body are arranged at one end of the casing body, another through hole is arranged on the wall surface of the other end of the casing body, a front and a rear blocking plates are respectively arranged in the both grooves on the casing body wall, gear racks are respectively arranged on the front and the rear blocking plates, another gear is arranged between the front gear rack and the rear gear rack, a third gear is arranged at one side of an axle and shares an identical axle with the gear between the two gear racks, and the ...

Multifunction test device for exhaust gas recirculation valve

The invention discloses a waste-gas recycling valve multi-function test apparatus. The gas outlet of a burner is successively connected with a cooler, a continuous rotary valve, one end of an overflow valve and the inlet of a waste-gas recycling valve under test; the gas inlet of a vacuum pump is successively connected with one end of the overflow valve, a two-position three-way valve and a vacuum tube of the waste-gas recycling valve under test; the outlet of the waste-gas recycling valve under test is successively connected with an exhaust cooler, a counterbalance valve, a high-temperature two-position three-way valve and a mass flowmeter, and then is filled with atmosphere; the water inlet of the cooler is successively connected with a speed-adjustable water pump and a cooling water tank; and the water outlet of the cooler is connected with a drain pipe. The invention can be used to test the flux characteristics of a waste-gas recycling pump, the dynamic characteristics of a vacuum waste-gas ...

Well test analysis control method for low permeability gas reservoir and method thereof

The invention provides a well test analysis control system for a low permeability gas reservoir and a method thereof. The method comprises the following steps: measuring bottom hole pressure value of a gas well in a period after well startup to obtain a relation value between pressure difference and time; judging whether the relation value equals a preset relation value, if yes, performing the next step, or correcting well test interpretation parameters corresponding to the preset relation value; taking the well test interpretation parameters corresponding to the preset relation value as the well test interpretation parameters of the gas well; analyzing gas permeation situation of the gas well according to the well test interpretation parameters so as to control exploitation of the well gas; wherein, the well test interpretation parameters comprise gas slip factor, low level permeability, skin factor, well bore storage coefficient, flow coefficient and formation pressure. The well testinterpretation ...

Exhaust gas recirculation valve and cooler combined test device

The invention discloses a test device for combining an exhaust gas recirculation valve with a cooler. Through a first three-way valve, a second three-way valve and a first four-way valve, a high-temperature gas device not only can be singly connected with a recirculation valve for exhaust gas to be tested, but also can be separately connected with a recirculation cooler for the exhaust gas to be tested, or can be jointly connected with the recirculation valve for the exhaust gas to be tested and the recirculation cooler for the exhaust gas to be tested; then through a second four-way valve and a fourth three-way valve, a high-temperature gas device is connected to a flow meter for measuring the flow. Meanwhile, two additional switch valves are installed in a pipeline, so as to control the precedence sequence during the combination measurement of the recirculation valve for the exhaust gas to be tested and the recirculation cooler for the exhaust gas to be tested. The test device can be ...

HIV stain drug-resistant phenotype analytical cell model and special pseudotype lentivirus therefor

The invention discloses a cell model of HIV strain phenotypic drug resistance analysis and special pseudotype slow virus thereof; the invention constructs plasmid for expressing Gag protein of HIV, recombinant slow-virus plasmid for expressing reporter gene and helper plasmid for expressing Rev protein of HIV; under the effect of the helper plasmid pVSV-G, the three plasmid and HIV reverse transcriptase and the gene fragment of protease which are amplified from strain can obtain the pseudotype slow virus of the HIV reverse transcriptase and protease gene containing strain. The pseudotype slowvirus is used for infecting the cells of mammals, thus obtaining the strain phenotypic resistance cell model based on the reporter gene. The cell model of the invention can carry out phenotypic resistance analysis to the strain, the reporter gene leads the cell model to have super high sensitivity, and the cell model of HIV strain phenotypic drug resistance analysis is applicable to the phenotypic drug ...

Engine oil heater for heat-tube engine

The invention discloses a device for heating engine oil, which comprises a heating section, a steam pipe, a condensing section, and the four-section pipe body of a condenser pipe, which are connected end to end. The heating device is characterized in that the heating section is attached on the outer surface of the exhaust pipe of the engine, and the condensing section is arranged on the bottom layer of the engine oil pan. By using the operating principle of the heating pipe, the waste heat of the engine exhaust can be transmitted to the oil of the engine oil pan, so that the problems that the engine oil is extremely viscous during the starting process in the severe cold regions or under the state of extremely low temperature and is not favorable for lubrication can be solved. The heating device has the advantages that the extra energy is not required to be consumed, the structure is simple, and the operation is convenient.