01-08-2023 дата публикации
Номер: CN116515869A
Принадлежит:
The invention discloses a 2, 4-diaminobutyric acid transferase mutant gene and application thereof, and the gene encodes a 2, 4-diaminobutyric acid transferase mutant with an amino acid sequence as shown in SEQ ID NO.2. The invention also discloses a preparation method of the 2, 4-diaminobutyric acid transferase mutant. The mutant gene is applied to synthesis of ectoine, firstly, expression cassettes of 2, 4-diaminobutyric acid transferase mutant gene EctBm, 2, 4-diaminobutyric acid acetyltransferase EctA and ectoine synthetase EctC are constructed, the 2, 4-diaminobutyric acid transferase mutant gene, the 2, 4-diaminobutyric acid acetyltransferase gene and the ectoine synthetase gene are co-expressed, and then the ectoine synthetase is synthesized. According to the method, 2, 4-diaminobutyric acid is used as a raw material to obtain a 2, 4-diaminobutyric acid transferase mutant, 2, 4-diaminobutyric acid acetyltransferase and ectoine synthetase, and on the basis, glucose or glycerol is ...
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