18-02-2015 дата публикации
Номер: CN104357450A
Принадлежит:
The invention provides an aphis gossypii spirotetramat resistance related CYP6A2 promoter and activity analysis, and belongs to the technical field of biological engineering. The sequence of the aphis gossypii spirotetramat resistance related CYP6A2 promoter is as shown in SEQ ID NO.1, wherein the region from -1bp to -489bp is the DNA sequence of the aphis gossypii spirotetramat resistance related CYP6A2 promoter, and the region from +1bp to +56bp is the DNA sequence of a 5' untranslated region of aphis gossypii P450 gene CYP6A2. The invention further provides a eukaryotic expression vector containing the sequence of the aphis gossypii spirotetramat resistance related CYP6A2 promoter for cell line transfection, and expression containing the sequence of the aphis gossypii spirotetramat resistance related CYP6A2 promoter for the eukaryotic expression vector. The promoter can be used for effective expression of eukaryotic genes and obtaining low abundance gene researches, so that high-level ...
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