Fluorescent probe in use for detecting glutathion inside cell, synthesis method and application

A method for preparing fluorescent probe enabling to detect glutathione in cell includes dissolving 150-200mg of Se-potassium hydride in 10mI of N, N-dimethyl formamide, adding 200-250mg of bromide reagent under room temperature and vacuum condition, adding 400-500mg of fluorescent dystuff and 500-600 micro-I of triethylamine as well as 150-190mg of cuprous iodide under inert gas protection, diluting reacted matter by acetic ether and using sodium hydroxide to wash it, separating mixture to obtain organic phase being dried by anhydrous magnesium sulfate, washing and drying solvent to obtain fluorescent probe.

Method for preparing dextran with Gluconobacter oxydans as strain

The invention discloses a dextran preparation method that uses Gluconabacterium axydans as a strain and comprises the following steps: (1) the Gluconabacterium axydans strain is cultivated on a slant culture medium; (2) culture fluid that is obtained by the step (1) is inoculated into a fermented culture medium and fermentation cultivated, thus obtaining fermented fluid that contains dextran dextrinase (DDase); and (3) the dextran dextrinase is extracted from the fermented fluid that is obtained in the step (2), and dextrine is transformed into the dextran through an enzyme transformation experiment. The dextran that is obtained by the dextran preparation method has lower viscosity and consequently has lower calorific value, and the dextran with low calorific value is called as an indigestible dextrine and is a water-soluble dietary fiber. With the characteristics of water holding capacity and viscosity, the indigestible dextrine can be used as a food additive with low calorific value and ...

Surface-enhanced Raman probe as well as preparation method and application thereof

The invention discloses a surface-enhanced Raman probe as well as a preparation method and application thereof, and relates to the field of biomedical imaging. The surface-enhanced Raman probe disclosed by the invention is mainly prepared from gold-silver core-shell nanoparticles modified by 4-mercaptophenylboronic acid pinacol ester molecules, and an enhanced Raman signal is obtained. The stability is improved by combining sulfydryl polyethylene glycol with a gold and silver shell layer, and the biological compatibility of the nano probe to normal cells is enhanced by a biological excitation protein coronal strategy. The specific response group is specific to peroxynitroso anions (ONOO <->). The synthesis steps of the surface-enhanced Raman probe are all carried out in an aqueous solution, and protein is used for wrapping, so that the biocompatibility of the existing surface-enhanced Raman probe can be improved, and the influence on the normal physiological function of the surface-enhanced ...

Fluorescent probe in use for detecting glutathion inside cell, synthesis method and application

A method for preparing fluorescent probe enabling to detect glutathione in cell includes dissolving 150-200mg of Se-potassium hydride in 10mI of N, N-dimethyl formamide, adding 200-250mg of bromide reagent under room temperature and vacuum condition, adding 400-500mg of fluorescent dystuff and 500-600 micro-I of triethylamine as well as 150-190mg of cuprous iodide under inert gas protection, diluting reacted matter by acetic ether and using sodium hydroxide to wash it, separating mixture to obtain organic phase being dried by anhydrous magnesium sulfate, washing and drying solvent to obtain fluorescent probe.

Micro-fluidic chip for multi-modal analysis of biological sample

The invention belongs to the field of design of micro-fluidic chips, and particularly relates to a micro-fluidic chip for multi-modal analysis of a biological sample, which comprises a pneumatic control chip layer, a fluid chip layer and a glass sheet layer, wherein the lower surface of the pneumatic control chip layer is attached to the upper surface of the fluid chip layer, and the lower surface of the fluid chip layer is arranged on the glass sheet layer; the pneumatic control chip layer is used for controlling the on-off of each channel in the fluid chip layer; the fluid chip layer is used for introducing a biological sample and a plurality of fluids in a detection process and carrying out multi-modal analysis in a limited range; and the glass sheet layer is used as a fixed carrier of the fluid chip layer and an attached carrier of a substrate of a detection area. According to the micro-fluidic chip disclosed by the invention, an antibody with immunocompetence is fixed on the chip by ...

Service discovery method and device, electronic equipment and storage medium

The embodiment of the invention provides a service discovery method and device, electronic equipment and a storage medium, and the method comprises the steps: obtaining a service list; storing the service list; obtaining a plurality of services in the service list and a connection weight of the current service; determining a target service to be connected with the current service in the plurality of services according to the connection weight; and establishing connection with the target service, and sending detection information to the target service. By implementing the above embodiment, decentralization can be realized, autonomous discovery between services can be realized without relying on a service center, and the communication quality is improved.

Near infrared fluorescent dye and its synthesis process

Method for detecting liquid aldehyde

The invention discloses a liquid aldehyde detecting method which adopts a fluorescent dye with amino groups as a fluorescent probe, and after the reaction is implemented under the acidulous, neutral or kalescent buffer liquid conditions and at the normal temperature for 15 to 60 minutes, the fluorescent intensity in a position with the largest emitting wavelength is detected. A solution without adding material to be detected is taken as the blank reference, and fluorescent signals are measured and found to be enhanced remarkably after the material to be detected, i.e. the isovaleric aldehyde is added, thus realizing the detection of the isovaleric aldehyde. The liquid aldehyde detecting method has high sensitivity, simple and convenient operation as well as quick response, and can be used for quick liquid aldehyde detection.

Photothermal-photodynamic antibacterial probe as well as preparation method and application thereof

The invention discloses a photo-thermal-photodynamic synergistic antibacterial multifunctional probe as well as a preparation method and application thereof, and relates to the field of biomedical antibiosis. The antibacterial probe comprises gold-silver alloy hollow nanoparticles and a photosensitizer modified on the surfaces of the gold-silver alloy hollow nanoparticles, and the photosensitizer for photodynamic antibacterial action is chlorin e6 (Ce6). The antibacterial probe disclosed by the invention is simple and convenient in synthesis steps, has excellent photo-thermal characteristics and photodynamic effect, has a remarkable effect on bacterial growth resistance, has good biocompatibility, and can be applied to the skin surface of animal tissues to achieve an antibacterial effect.

Pincer-shaped skeleton ligand containing phosphine bond and preparation of ligand, and preparation method of Pincer-shaped organic compound

The invention relates to preparation of a Pincer-shaped metallic organic compound, and particularly relates to a pincer-shaped skeleton ligand containing a phosphine bond and preparation of the ligand, and a preparation method of the Pincer-shaped organic compound. The structural formula of the ligand is shown in a formula 1 described in the specification, wherein R1, R2, R3 and R4 can be hydrogen or methyl. The ligand is simple in synthetic process, low in cost, high in yield, easily available in raw materials, and mild in reaction conditions. The chemical formula of the Pincer-shaped metallic organic compound formed by the pincer-shaped skeleton ligand containing the phosphine bond and cobalt atoms is C35H34Cl2CoO2P2, and the structural formula of the Pincer-shaped metallic organic compound can be abbreviated into (DPPM)CoCl2, wherein DPPM is 2,4-diphenyl dibenzyloxy phosphoiyl methylene mesitylene, the molecular weight is 678.43, the DPPM belongs to a triclinic system, the space group ...

Method for separating and purifying dextran dextrinase from gluconobater oxydans

The invention relates to a method for separating and purifying dextran dextrinase from gluconobater oxydans, comprising the following steps of: firstly, collecting thalli by centrifugation, suspending by using phosphate buffer, crushing cells by using ultrasonic waves, , collecting a supernatant by centrifugation; and finally carrying out protamine sulfate precipitation, ammonium sulfate precipitation, hydrophobic chromatography and gel chromatography to obtain the high-purity dextran dextrinase by separation. The method has the advantages of simplicity, good repeatability and high protein yield.

Method for quickly extracting and determining dextran dextrinase in cells of gluconobacter oxydans

The invention discloses a method for quickly extracting and determining dextran dextrinase in cells of gluconobacter oxydans, which comprises the following main steps: centrifugating gluconobacter oxydans fermentation liquid to collect thalli; after washing the thalli with phosphate buffer, carrying out permeabilization treatment on the cells by a proper amount of glycine+Triton X-100 to release the protein in the cells; and then determining the enzymatic activity of the dextran dextrinase translating nitrobenzene-alpha-D-glycopyranoside (NPG) for generating p-nitrophenol. The method of the invention has simple operation and good repeatability and parallelization, and can be used for quick extraction and activity detection of the dextran dextrinase in the cells of the gluconobacter oxydans.

Near-infrared small-molecule fluorescent probe as well as preparation method and application thereof

The invention discloses a near-infrared small-molecule fluorescent probe as well as a preparation method and application thereof, and relates to the field of biomedical imaging. The near-infrared small-molecule fluorescent probe disclosed by the invention is mainly used for carrying out specific response on hydrogen peroxide (H2O2). According to the near-infrared small-molecule fluorescent probe disclosed by the invention, the dicyandiamide-methyl benzopyran is mainly used as a near-infrared luminophore, and the pentafluorobenzene sulfonyl ester is used as a response group, so that the near-infrared small-molecule fluorescent probe makes specific response to H2O2. Due to the introduction of dicyandiamide-methyl benzopyran, the probe has large Skke dragging displacement, and spectrum overlapping is effectively avoided. The near-infrared small-molecule fluorescent probe disclosed by the invention is simple and convenient in synthesis steps, has excellent spectral characteristics and good ...

Preparation method and application of multi-charge electrostatic self-assembled nano composite material

The invention discloses a preparation method and application of a multi-charge electrostatic self-assembled nano composite material, and relates to the technical field of nano materials. Comprising the following steps: (1) synthesizing CAT-Ce6; (2) synthesis of negative electricity Ag2S-3MPAQDs (silver < 2 > S-3 > MPAQDs); (3) synthesis of positively charged Ag2S-NH2QDs is carried out; and (4) preparation of the Ag2S (at) CAT-Ce6 (at) Oxa nano composite material: carrying out ultrasonic mixing on the Ag2S-NH2QDs solution and the CAT-Ce6 solution to obtain a mixed solution, dissolving Oxa by using a mixture of methanol and DMSO, dropwise adding the dissolved Oxa into the mixed solution, stirring in a room-temperature dark environment to obtain an Ag2S (at) CAT-Ce6 (at) Oxa crude product, and purifying the Ag2S (at) CAT-Ce6 (at) Oxa crude product to obtain an Ag2S (at) CAT-Ce6 (at) Oxa nano particle pure product. Controllable release of the medicine can be achieved, and meanwhile the curative ...