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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 8. Отображено 8.
04-08-2023 дата публикации

Efficient and accurate multi-gene editing system mediated by replicative guide editor

Номер: CN116536352A
Автор: ZHAO SHUHONG, RUAN JINXUE, ZHAO GUANGXING, LI XINYUN, HAN XIAOSONG, XIONG YOUCAI, SU YINYU, XIE SHENGSONG
Принадлежит:

The invention discloses an efficient and accurate multi-gene editing system mediated by a replicated guide editor. The invention provides a vector for multi-gene editing. The vector can express fusion protein, EBNA1 protein and Csy4 protein, wherein the fusion protein is formed by fusion of Cas9 cutting enzyme or a variant thereof and reverse transcriptase or a variant thereof, and the EBNA1 protein and the Csy4 protein are formed by fusion of Cas9 cutting enzyme or a variant thereof and reverse transcriptase or a variant thereof. The primer group contains an Orip element and a region for inserting a pegRNA coding sequence; the region for inserting the pegRNA coding sequence contains an insertion site and a promoter located at the upstream of the insertion site. Compared with a traditional method, the method has the advantages that multiple genes can be accurately and efficiently edited at a time, and the method has wide application prospects in the fields of gene therapy, construction ...

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Номер записи: 1
04-08-2023 дата публикации

Replication type efficient guide editing system

Номер: CN116536353A
Автор: RUAN JINXUE, ZHAO SHUHONG, HAN XIAOSONG, LI XINYUN, ZHAO GUANGXING, XIONG YOUCAI, SU YINYU, XIE SHENGSONG
Принадлежит:

The invention discloses a duplicated efficient editing guiding system. The invention provides a vector for gene editing. The vector can express a fusion protein formed by fusing a Cas9 cutting enzyme or a variant thereof and a reverse transcriptase or a variant thereof, and an EBNA1 protein or a mutant EBNA1 Mut thereof; the primer pair contains an Orip element and a region for inserting a pegRNA coding gene; the region for inserting the pegRNA coding gene contains an insertion site and a promoter located at the upstream of the insertion site. According to the constructed additive guided editing system carrier, the editing efficiency is improved by prolonging the editing time, and insertion mutation caused by exogenous gene integration is avoided. Compared with other existing systems, the system integrates the characteristics of high efficiency, safety and accuracy which are difficult to be simultaneously realized by other systems, and lays a foundation for application of the guide editing ...

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Номер записи: 2
23-06-2023 дата публикации

Method for calculating water consumption of sparsely planted crops under pipeline damage condition

Номер: CN116307610A
Автор: ZHANG HENGJIA, BAI YOUSHUAI, XIONG YOUCAI, YU SHOUCHAO, WANG ZEYI, WANG YINGYING, WANG YONG
Принадлежит:

The invention relates to the technical field of agricultural water management, in particular to a sparse-planting crop water consumption calculation method under a pipeline damage condition, which comprises the following steps of: determining a growth period or a time period at the beginning and end of the growth period of a sparse-planting crop; determining the soil moisture content at the two time points through a soil drilling method, and calculating the soil water storage capacity of the sparse-planted crops; measuring the irrigation amount of a single sparse-planted crop by using a water meter; determining a water leakage point and a water leakage amount of each damaged pipeline; according to the method for calculating the water consumption of the sparse-planted crops under the pipeline damage condition based on the water balance principle, the water consumption of the sparse-planted crops is accurately determined, reliable water utilization requirements are provided for a water utilization ...

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Номер записи: 3
04-06-2014 дата публикации

Method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi

Номер: CN103828618A
Автор: ZHU YING, XIONG YOUCAI, CHEN YINGLONG, WANG ZHAOBIN, WANG JIANYONG
Принадлежит:

The invention discloses a method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi to bring the reciprocal symbiosis relation between AM fungi and host plants under drought stress into play reasonably and efficiently. According to the method, the AM fungi having a close symbiotic relation with the wheat are selectively used, and the symbiotic relation between the AM fungi and the wheat is built in a condition control mode to promote the wheat growth and improve drought adaptability. The AM fungi comprise one of glomus monosporum and acaulospora laevis, the two kinds of AM fungi are respectively propagated in an expanding mode in an expanding propagation matrix by using red clovers, and then two AM fungi inoculants and the wheat are cultured together in a symbiotic culture medium. The effective path for profitable symbiosis of the AM fungi and the host plants in adversity conditions is provided, the infection and symbiosis relation with the ...

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Номер записи: 4
18-07-2023 дата публикации

Water and fertilizer integrated drip irrigation device

Номер: CN116439111A
Автор: ZHANG HENGJIA, PAN XIAOFAN, XIONG YOUCAI, YU SHOUCHAO, WANG SHUOSHUO, CHEN TAO
Принадлежит:

The invention provides a water and fertilizer integrated drip irrigation device, and relates to the field of irrigation, the water and fertilizer integrated drip irrigation device comprises a mobile vehicle, the top of the mobile vehicle is fixedly connected with a modulation box, the top of the mobile vehicle is provided with a second water pump connected with the modulation box, the output end of the second water pump is provided with a fertilizer water pipe, and one end of the fertilizer water pipe is provided with a plurality of positioning cylinders; a water tank is fixedly connected to the top of the moving vehicle, a water outlet pipe connected with the positioning cylinders is arranged on the outer side of the water tank, auxiliary assemblies used for assisting in supporting the positioning cylinders are arranged on the outer sides of the multiple positioning cylinders, mixing cylinders are fixedly connected to the interiors of the multiple positioning cylinders, and first water ...

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Номер записи: 5
09-05-2023 дата публикации

Under-mulch drip irrigation water-nitrogen coupling irrigation fertilization method

Номер: CN116076218A
Автор: ZHANG HENGJIA, ZHOU CHENLI, XIONG YOUCAI, YU SHOUCHAO, CHEN XIETIAN, WANG YINGYING, WANG YONG, LIU LINTAO
Принадлежит:

The invention provides an under-mulch drip irrigation water-nitrogen coupling irrigation fertilization method, which relates to the field of under-mulch drip irrigation, and comprises the following steps: ditching in a planting area to apply a base fertilizer, and ridging after raking and leveling; laying drip irrigation belts between adjacent ridges, and covering the drip irrigation belts with a mulching film; transplanting eggplant seedlings on the ridges; different irrigation upper and lower limits are set in different growth periods of eggplant seedlings, irrigation treatment is conducted in the seedling period, the flowering and fruit setting period, the full bearing period and the consequence period of the eggplant seedlings through drip irrigation belts, and irrigation should be conducted to the upper limit immediately when the soil moisture content is low to the lower limit. According to the under-mulch drip irrigation eggplant water nitrogen deficit regulation irrigation fertilization ...

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Номер записи: 6
25-04-2023 дата публикации

Method for efficient gene editing through replicating type dCas9-FokI system

Номер: CN116004716A
Автор: RUAN JINXUE, ZHAO SHUHONG, SU YINYU, LI XINYUN, XIONG YOUCAI, HAN XIAOSONG, HE RUIGAO, WANG XIAOXUAN, XI XIAONING, YANG ZHIYUAN, YU MEI, XIE SHENGSONG, LIU XIANGDONG
Принадлежит:

The invention discloses a method for carrying out efficient gene editing by utilizing an ep-dCas9-FokI system, and particularly discloses a method for carrying out gene editing by mediating fusion protein dCas9-FokI expression through an attachment element (EBNA1/oriP), and the ep-dCas9-FokI system generates cut FokI nuclease in a dimerization form, so that a recognition sequence of sgRNA is not damaged, and the gene editing efficiency is improved. And continuously expressing dCas9-FokI nuclease through mediation of an EBNA1/oriP attachment, and continuously cutting a sequence which is not knocked out, so as to achieve the purposes of improving the knockout efficiency and increasing repeated editing of a target site which is not subjected to target editing for multiple times. By utilizing the method, the specific nucleotide sequence can be efficiently knocked out, and a feasible method and a powerful gene editing tool are provided for gene function research.

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Номер записи: 7
01-08-2023 дата публикации

Pumpkin water nitrogen deficiency regulating method

Номер: CN116508466A
Автор: ZHANG HENGJIA, LIU LINTAO, XIONG YOUCAI, YU SHOUCHAO, CHEN XIETIAN, WANG YINGYING, WANG YONG, ZHOU CHENLI
Принадлежит:

The invention provides a pumpkin water and nitrogen deficiency regulating method, which relates to the field of agriculture and comprises the following steps: S1, collecting a conventional water and nitrogen management method of farmers in a region and average rainfall of pumpkins in a conventional hydrological year in a growth period; s2, carrying out a long-term positioning test of different water and nitrogen management methods of pumpkins; s3, pumpkin planting; s4, establishing a regression equation of pumpkin yield, nitrogen fertilizer utilization rate and water utilization rate under different water and nitrogen management methods in the region; on the basis of a pumpkin water and nitrogen management long-term positioning test, data such as conventional hydrological annual rainfall, an irrigation mode, pumpkin yield, water utilization rate and nitrogen fertilizer utilization rate in a test area are investigated and analyzed; by means of a numerical simulation method, an optimal pumpkin ...

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Номер записи: 8