Whole-cell utilization method of beer yeast as well as product and application of whole-cell utilization method

The invention discloses a beer yeast whole-cell utilization method and a product and application thereof, and relates to the technical field of bioengineering, and the method comprises the following steps: pretreatment, yeast wall breaking, extraction of yeast nucleotide from an endolysate supernatant system, and simultaneous extraction of glucan, mannan oligosaccharide and other zymosan products from a precipitation system. The zymosan and the yeast nucleotide obtained by the method can be used as food additives and flavoring substances. According to the method, multiple zymosan and yeast nucleotide products are simultaneously separated and extracted in one technological process by using the eutectic solvent, and the yeast cell wall polysaccharide is extracted in a graded manner at normal temperature by using the eutectic solvent, so that the use amount of alcohol is reduced, and the risk in the alcohol precipitation separation process in industrial production can be reduced; the eutectic ...

Disotropic agent for disturbing mating of tomato leaf miner adults and application of disotropic agent

The invention discloses a disruption agent for disturbing mating of tomato leaf miner adults, and belongs to the field of biological control. The disruption agent comprises an active component and a slow release agent. The component of the tomato leaf miner isotropic agent is (3E, 8Z, 11Z)-tetradecatriene acetate, and the slow release agent is corn oil or sunflower seed oil. The isotropic agent components and the slow-release agent are mixed to prepare mother liquor, and the mother liquor is added into a polyethylene capillary tube to prepare the tomato leaf miner isotropic silk. The mating disruption agent can significantly reduce the encounter probability of male and female adults and effectively reduce the mating rate of the male and female adults, and generally, the mating rate can be reduced by 90% or above. Meanwhile, the isotropic agent is high in specificity, long in lasting period, safe to natural enemies and environment-friendly, and has important significance on green prevention ...

Spaceborne target detection tracing camera in sun viewing blind zone

Compounds and uses thereof

The present invention relates to methods and compositions for treating BAF-related conditions, such as cancer and viral infections.

Method for measuring volatile fatty acid in fat tissue

The invention discloses a method for measuring volatile fatty acid in fat tissue, which comprises the following steps: A1: saponifying a fat tissue sample; A2: separating the volatile fatty acid from the saponified product obtained in the step A1 by using a distillation extraction device; A3: concentration; and A4: gas chromatography. Based on the prior art, in the invention, the distillation extraction and gas chromatography techniques are combined, the operation steps aiming at the feature of the volatile fatty acid are properly improved, the original fatty acid extraction process is omitted, gas chromatography is directly performed on the separated volatile fatty acid, thus the method has no derivatization; a set of simple, convenient and economic method for measuring volatile fatty acid in fat tissue is established which lays a foundation for deeply researching the volatile fatty acid in fat tissue.

Method for adjusting fluorescence color of one-dimensional perovskite single crystal

The invention provides a simple and easy-to-operate method for adjusting fluorescence emission color of one-dimensional perovskite, which comprises the following steps of: 1) synthesizing manganese-doped perovskite C4N2H14PbBr4 one-dimensional perovskite by using a novel method, specifically comprising the following steps of: (1) adding PbBr2, MnBr2. 4H2O and N, N '-dimethylethylenediamine (DMEDA) into hydrobromic acid and continuously stirring, and (2) putting a mixed solution into an acetone steam diffusion chamber, standing and drying; and 2) exciting the obtained crystal by using laser, and changing the fluorescence emission color of the perovskite single crystal by changing the repetition frequency and pulse energy of the laser. The raw materials used in the adjusting mode are not high in cost, operation is easy, the requirement for equipment is low, and a good effect on the society and the environment is expected to be generated.

Rapid screening and evaluation method suitable for seaweed near-water beverage raw materials

The invention provides a rapid screening and evaluation method suitable for seaweed near-water beverage raw materials, and belongs to the technical field of food. According to the method, the seaweed raw materials suitable for the near-water beverage are comprehensively evaluated for the first time, the next production process can be formulated according to the evaluation result, and therefore it is guaranteed that the seaweed near-water beverage is clear and transparent in appearance, free of peculiar smell and remarkable in effect; besides, the method covers evaluation of the raw materials, evaluation of the initial beverage and evaluation of the whole shelf life of the beverage, the evaluation stage is more comprehensive, a technical basis is provided for comprehensively guaranteeing the product quality, and the problem that a traditional seaweed enzyme powder evaluation technology only passes qualitative evaluation, and the evaluation efficiency is low is effectively solved. The requirements ...

Method for detecting taurine in functional beer by adopting high performance liquid chromatography (HPLC)

The invention discloses a method for detecting taurine in functional beer by adopting a high performance liquid chromatography (HPLC), comprising the steps: (1) a derivation pretreatment procedure: carrying out pre-column derivation on 6-aminoquinoline-N-hydroxysuccinimido formate; (2) a gradient elution procedure: regulating the pH value of a mobile phase A to 4.98 by using 50% phosphoric acid, and optimizing the gradient elution procedure aiming at a standard sample and a beer sample respectively so as to completely separate the taurine from other interfering substances in the beer, whereinthe mobile phase A is a buffer solution including 139.92mmol/L of sodium acetate trihydrate and 16.39mmol/L of triethylamine, a chromatographic column used for sample analysis is an AccQ-Tag amino acid analysis column C18 of the American Waters company, and the chromatographic column is in the specification of 3.9mm*50mm and 4mum; and (3) a data treatment procedure: comparing beer to be detected with ...

Method for flocculating concentrate obtained by floating low-quality manganese carbonate ore

The invention discloses a method for flocculating concentrate obtained by floating low-quality manganese carbonate ore. In the method disclosed by the invention, cation 1200 at a concentration of 0.5g/L to 1.5g/L is selected to flocculate concentrates, and an ideal effect is achieved.

Regulation and control method for near-water beverage based on seaweed meal raw material performance

The invention provides a near-water beverage regulation and control method based on seaweed meal raw material performance, and belongs to the technical field of food. According to the regulation and control method provided by the invention, the raw materials of the seaweed near-water beverage are analyzed on the basis of appearance, sense, turbidity and functional components, and corresponding evaluation results are obtained; in combination with the evaluation result, the sense and turbidity of the raw materials of the seaweed near-water beverage are regulated and controlled, so that the raw materials with different quality indexes all meet the product requirements. According to the method, the raw materials with different quality indexes can meet the product requirements in combination with the raw material grading condition, the consistency of the product quality can be ensured based on raw material prediction, a technical basis is provided for comprehensively ensuring the product quality ...

Method for constructing gastric cancer prognosis model based on basilar membrane related gene

The invention belongs to the technical field of biology, and particularly relates to a method for constructing a gastric cancer prognosis model based on a basilar membrane related gene, and the method comprises the following steps: S1, data acquisition; s2, screening differential expression mRNA (messenger ribonucleic acid); s3, performing functional enrichment analysis; s4, establishing and evaluating a risk score based on the BMRGs; s5, performing gene set enrichment analysis (GSEA); s6, immune infiltration analysis; s7, potential drug prediction and drug sensitivity analysis; s8, statistical analysis; based on TCGA data and a bioinformatics technology, a risk scoring model of BMRGs is established, clinical pathology data is combined, the prognosis function, a possible molecular mechanism and a corresponding tumor microenvironment are discussed, potential target drugs are predicted, and theoretical support is provided for clinical decision-making of gastric cancer.

Analysis method for detecting furans compounds and pyrans compounds in beer

The invention discloses an analysis method for detecting furans compounds and pyrans compounds in beer, which combines a solid-phase extraction analysis method and a gas chromatogram/mass spectrometry analysis method. The analysis method comprises the following steps of preparation, solid-phase extraction, gas chromatogram/mass spectrometry analysis, detection and the like. The analysis method for detecting volatile furans compounds and pyrans compounds in beer is established and has the advantages of rapidness, small sample usage, accuracy, sensitiveness, high efficiency and the like. Small polystyrene/divinyl benzene columns selected in the solid-phase extraction process have the characteristics of strong adsorption capability, small organic solvent usage, convenience, safety, high efficiency and the like.

Ultra-clean sterilization device for biological information kidney bean gene breeding appliance

An ultra-clean sterilization device for a biological information kidney bean gene breeding appliance relates to the technical field of sterilization and disinfection devices and comprises a sterilization box and a vaporization box, and a moist heat sterilization assembly is arranged in the sterilization box; the moist heat sterilization assembly comprises a liftable and rotatable isolation net cylinder, the isolation net cylinder comprises a mounting bottom plate and a mounting top plate which are horizontally arranged, a plurality of centrosymmetric supporting columns are arranged between the mounting top plate and the mounting bottom plate, and an arc-shaped filter screen is fixedly connected between every two adjacent supporting columns; a rotary clamping seat is fixedly connected to the center of the lower surface of the mounting bottom plate, and a cross-shaped rotary clamping groove is formed in the lower surface of the rotary clamping seat; a cross-shaped limiting clamping block ...

Graded screening device for tomato seed processing

A grading screening device for tomato seed processing relates to the technical field of seed screening and comprises a hopper, a stirring mechanism is arranged in the hopper, two groups of symmetrical mounting support frames are fixedly connected to the lower end of the hopper, and a secondary separation box is transversely fixed between the two groups of mounting support frames; two symmetrical suspension mounting plates are fixedly connected to the side end face of the lower portion of the hopper, the primary screening box is fixed between the two suspension mounting plates in the transverse direction, an airflow screening assembly is arranged in the primary screening box, and a vibration sorting assembly is arranged in the secondary separation box. A vertical transfer communicating pipe is fixedly connected into an opening in the lower end of the hopper, the lower end of the transfer communicating pipe extends into the primary screening box, and a flow dividing buffering mechanism is ...

Whole-wheat barreled strong lager beer and preparation process thereof

The invention provides wholewheat barreled strong lager beer and a preparation process thereof, and belongs to the technical field of beer brewing. According to the wholewheat barreled strong lager beer provided by the invention, on the premise of high alcoholic strength (greater than or equal to 10% vol), the strong lager beer which is rich in flavor, integrates in-barrel flavor substances absorbed during barreling, is complex in taste and strong in layering sense, and breaks through the degree and storage time limit of traditional beer is developed through process technology innovation; and high-difficulty innovative challenge of high-alcohol beer is realized.

High-alcoholic-strength red lager beer and preparation method thereof

The invention provides high-alcoholic-strength red lager beer and a preparation method thereof, and belongs to the technical field of beer brewing. The invention provides high-alcoholic-strength red lager beer and a preparation method thereof, which are mainly adjusted from three aspects of malt formula, wort preparation and beer fermentation. According to the method, through a process innovation technology, the strong lager beer which is beautiful in color, rich in flavor and complex in taste on the premise of high alcoholic strength is obtained. The prepared high-alcoholic-strength red lager beer has the alcoholic strength of 8% vol-9% vol, the red intensity larger than or equal to 0.2, the color of L60-70/a 20-30/b 80-90 and the chromaticity of 25-30 EBC, has the flavors of caramel fragrance, hazelnut fragrance and the like, the original wort concentration of the beer is 18-20 degrees P, the bitterness quality is 20-50 BU, the carbon dioxide content is 0.50-0.60% mass, the dimethyl furanone ...

Two-machine hot standby switching circuit

The invention discloses a two-machine hot standby switching circuit. The two-machine hot standby switching circuit comprises an isolating circuit, a switching interlocking circuit and an active/standby indicating circuit, wherein the switching interlocking circuit comprises two relays, each relay comprises three contact switches, one of the three switches is normally closed and the other two of the three switches are normally open. Isolated system A active striving signals are connected to a first end of a coil of a system A relay through the normally closed switch of a system B relay, and a second end of the coil of the system A relay is connected with a system A control power supply; isolated system B active striving signals are connected to a first end of a coil of the system B relay through the normally closed switch of the system A relay, and the second end of the coil of the system B relay is connected with a system B control power supply; system A active/standby indicating signals ...

Wafer slip risk assessment method and chemical mechanical polishing device

The invention provides a wafer slip risk assessment method and a chemical mechanical polishing device, and the method comprises the steps: S100, collecting friction torques of a gas film and a retaining ring, and respectively recording the friction torques as torqueA and torqueB; assigning a value to the average friction force fRR of the retaining ring, enabling the total friction torque to be equal to torqueB, and calculating the lateral force FRR and the friction coefficient mu s borne by the retaining ring according to the fRR; assigning a value to the average friction force fw of the gas film, enabling the total friction torque to be equal to torqueA, and calculating the lateral friction force FW and the friction coefficient mu k borne by the wafer according to the fw; defining boundary conditions a and b, letting a = [mu] s-[mu] k and b = FRR-FW, if alt; -0.5 and/or blt; and-1500, the wafer has the risk of sliding. According to the slip sheet evaluation method provided by the invention ...