Recombinant adenovirus expressing E0, E2 gene of classical swine fever virus

The invention provides recombinant adenovirus expressing E0, E2 genes of classical swine fever virus, which is characterized in that the E0 gene of classical swine fever virus is inserted into Bgl II and Kpn I enzyme sites of a multiple cloning site at downstream of adenovirus CMV promoter, and a adenovirus CMV promoter and a CMV-E2 segment of the E2 gene of classical swine fever virus are inserted into Xho I and Xba I enzyme sites. The invention solves the problems of low yield and weak protective effect of the fusion tandem expression of the E0, E2 genes, allows an immunized swine to obtainan antibody against classical swine fever virus only by immunization once, and the production cost is half of that of conventional vaccines; the recombinant adenovirus belongs to non-replicative virus, and does not propagate in swine; the phenomenons of virus atavism and virulence enhancement due to the long-term inoculation of attenuated vaccine are solved. The recombinant adenovirus only comprises ...

Recombinant adenovirus and construction method thereof

The invention provides a recombinant adenovirus and a construction method thereof. The construction method comprises the following steps of: inserting swine fever virus E2 genes into a multiple cloning site Bg1 II and a Sa1 I enzyme cutting site on the downstream of a CMV (cytomegalovirus) promotor of the adenovirus; and inserting porcine reproductive and respiratory syndrome virus GP5 genes into enzyme cutting sites Kpn I and Not I on the downstream of the CMV promotor of the adenovirus. The problems of low expression yield and weak protection effect when the E2 and GP5 genes are fused and connected in series are solved. Only by once immune vaccination, a pig can obtain antibodies for a swine fever virus and a porcine reproductive and respiratory syndrome virus. The recombinant adenovirus is a nonreplicative virus and is not reproduced in a pig body. The phenomena of virus atavism and toxicity strengthening caused by long-term vaccination of an attenuated vaccine are avoided. Moreover, ...

Recombinant adenovirus expressing E0, E2 gene of classical swine fever virus