29-08-2023 дата публикации
Номер: CN116649220A
Автор:
GONG JIANYING,
WANG HUAXIN,
WANG XIAOYU,
SUN LINA,
WANG ZHUNA,
PAN WEN,
WEI WEI,
WU JIANYUN,
LIANG TENGCHENG,
LI LIGANG,
HU WEIPIN,
YANG LIPING,
YI SHIBIN,
LIANG XIUHAO,
CHEN JIANWU
Принадлежит:
The invention discloses a dendrobium tissue culture seedling in-vitro preservation method which comprises the following steps: firstly, performing growth inhibition treatment on differentiated and rooted dendrobium tissue culture seedlings, and then putting the dendrobium tissue culture seedlings into a growth culture medium for growth recovery, the formula of the growth culture medium comprises 0.03 mg/L of 1/2MS + NAA, 1.0 g/L of peptone, 1.0 g/L of activated carbon, 25 g/L of cane sugar, 6.0 g/L of agar, 50 g/L of potatoes and 80 g/L of bananas; the formula of the inhibition culture medium for inhibiting growth comprises 60-100g/mL of coconut juice and 6g/L of agar. According to the method, through the methods of utilizing organic additives, preparing a proper culture medium, adjusting the PH value and the like, in the growth inhibition period, plants are short, pseudobulbs are thick and strong, the number of leaves is moderate, the number of new buds is small, the proliferation rate ...
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