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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 5. Отображено 5.
12-08-2021 дата публикации

Cell strain constructing method for preparing fully human anti-EGFR monoclonal antibody

Номер: AU2019422095A1
Автор: LI MINGLI, YIN CHUNGUANG, LI MINGXUAN, DENG CHANGJIANG, XING JINHUA, NIU CHUANDONG, ZHOU XIAOMENG, LI, Mingli, YIN, Chunguang, LI, Mingxuan, DENG, Changjiang, XING, Jinhua, NIU, Chuandong, ZHOU, Xiaomeng
Принадлежит:

Provided is a cell strain constructing method for preparing fully human anti-EGFR monoclonal antibody, wherein, in a POOL screening step after cells of dihydrofolate reductase negative Chinese hamster ovary cell lines (CHO/DHFR-) complete a transfection step, in the first stage, the cell lines integrated with the heavy chain gene are screened using a SFM4CHO culture medium without HT, and the cell lines integrated with the light chain gene are screened simultaneously using a Blasticidin in the culture medium; and in the second stage, MTX is added to increase copy numbers of the light and heavy chain genes integrated into the cell line genomes to improve the expression level. A stable cell line with an expression level of 2.0g/L-3.0g/L, which is suitable for a large trial production process, is cloned and screened out with improved product SEC, pI-cIEF, de-sugaring molecular weight and antibody activity based on the cells.

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Номер записи: 1
09-09-2021 дата публикации

ESTABLISHMENT METHOD OF CELL LINE FOR PREPARING ANTI-EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR) FULLY-HUMANIZED MONOCLONAL ANTIBODY (mAb)

Номер: AU2019101814A4
Автор: LI MINGLI, YIN CHUNGUANG, LI MINGXUAN, DENG CHANGJIANG, XING JINHUA, NIU CHUANDONG, ZHOU XIAOMENG, LI, Mingli, YIN, Chunguang, LI, Mingxuan, DENG, Changjiang, XING, Jinhua, NIU, Chuandong, ZHOU, Xiaomeng
Принадлежит:

The present disclosure provides an establishment method of a cell line for preparing an anti epidermal growth factor receptor (EGFR) fully-humanized monoclonal antibody (mAb), where dihydrofolate reductase (DHFR)-deficient Chinese hamster ovary (CHO) cell lines CHO/DHFR- are subjected to POOL screening after undergoing transfection, and the POOL screening includes: stage 1, using an HT-free SFM4CHO medium to screen out a cell line integrated with a heavy chain (HC) gene, and using Blasticidin in the medium to screen out a cell line integrated with a light chain (LC) gene; and stage 2, adding MTX to increase copy numbers of the LC gene and the HC gene that are integrated into a genome of a cell line, thereby increasing an expression level. A stable cell line with an expression level of 2.0 g/L to 3.0 g/L suitable for large-scale production processes is cloned and screened out, and the SEC, pI-cEF, deglycosylated molecular weight, and cell-based antibody activity of a product are improved ...

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Номер записи: 2
27-05-2009 дата публикации

Disease-resistant breeding method for avian influenza and special reagent kit therefor

Номер: CN0101440409A
Автор: DU LIXIN, LI HONGBIN, YIN CHUNGUANG, LIXIN DU, HONGBIN LI, CHUNGUANG YIN
Принадлежит:

The invention discloses a disease-resistant breeding method for chicken avian influenza, and a special kit. The method comprises the steps of detecting whether 2216th-position deoxyribonucleotide from a 5' end of a nucleotide sequence shown by a sequence 1 in a sequence table of a to-be-detected chicken is G or A, determining the genotype of the to-be-detected chicken is AA, BB or AB and adopting an AA genotype chicken for breeding, wherein an AA genotype is homozygote of the 2216th-position deoxyribonucleotide from a 5' end of a nucleotide sequence shown by a sequence 1 in a sequence table, which is A; the BB genotype is the homozygote of the 2216th-position deoxyribonucleotide from a 5' end of a nucleotide sequence shown by a sequence 1 in a sequence table, which is G; and the AB genotype is heterozygote of A and G. The method can screen out a strain having better capability of resisting avian influenza, and has great economic significance and practical significance for breeding poultry ...

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Номер записи: 3
05-05-2023 дата публикации

Occult blood appearing reagent based on aggregation-induced emission molecules as well as preparation method and application of occult blood appearing reagent

Номер: CN116067926A
Автор: TANG BENZHONG, YIN CHUNGUANG, HAO BIN, LIU YONG, WANG ZHIMING, XIE SHENG, LI CHUNYAN
Принадлежит:

The invention relates to an occult blood appearing reagent based on aggregation-induced emission molecules as well as a preparation method and application of the occult blood appearing reagent. The occult blood appearing reagent prepared by the invention consists of a reagent A and a reagent B, wherein the reagent A contains aggregation-induced emission developing molecules, a thickening agent, a stabilizing agent and water. The reagent B is a hydrogen peroxide solution. The reagent does not destroy the displayed blood DNA, and is wide in application range. In the occult blood appearing process, the reagent is oxidized by nascent oxygen generated by hydrogen peroxide catalyzed by ferriporphyrin in blood, so that fluorescence is quenched, and change of macroscopic absorption color is generated at the same time. The method disclosed by the invention fills up the blank of no reagent for identifying occult blood by utilizing fluorescence and absorption color change in the current market. Compared ...

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Номер записи: 4
04-08-2023 дата публикации

Method and system for optimizing data of FlinkSQL repeated consumption data source

Номер: CN116541413A
Автор: LI LIN, YIN CHUNGUANG, ZHANG LUBO, CONG GUANGHUI
Принадлежит:

The invention discloses a method and system for optimizing FlinkSQL repeated consumption data source data, and relates to the technical field of financial payment. The method comprises the following steps: when an Flink Job is submitted by an Flink Client, obtaining a generated StreamGraph (StreamGraph); and analyzing whether the generated StreamGraph satisfies a pruning condition, if so, performing pruning optimization on the StreamGraph to generate a new StreamGraph, and submitting the new StreamGraph to a cluster for operation. According to the method and the device, the problem of repeated consumption of data of the data source can be avoided when the FlinkSQL operates a plurality of SQL at the same time.

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Номер записи: 5