Strain SRB-1 for anaerobic degradation of herbicide butachlor and application thereof

The invention discloses a strain SRB-1 for anaerobic degradation of a herbicide butachlor and application of the strain SRB-1, the strain is Terrisporobacter sp.SRB-1, is a gram positive bacterium, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No.40301. The invention further discloses a preparation method of the strain SRB-1 for anaerobic degradation of the herbicide butachlor and application of the strain SRB-1 for anaerobic degradation of the herbicide butachlor. The anaerobic degradation half-life period of the strain on butachlor with the concentration of 50 mg/L is 2.33 d, and the strain can be used for quickly and efficiently degrading the butachlor in an anaerobic manner; meanwhile, a good bioremediation effect is achieved on pollution of butachlor in industrial sewage or pollution of butachlor in the natural environment, the bioremediation advantage which is not achieved by aerobic microorganisms is achieved ...

Strain SRB-6 and application thereof in anaerobic degradation of herbicide acetochlor

The invention discloses a strain SRB-6 and an application of the strain SRB-6 in anaerobic degradation of a herbicide acetochlor, the strain is Cupidesulfovibriosp.SRB-6, is gram-negative bacteria, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No.40303. The invention further discloses a preparation method of the strain SRB-6. The strain SRB-6 can be applied to anaerobic degradation of the herbicide acetochlor. Under the anaerobic condition, the degradation half-life period of the strain on acetochlor with the concentration of 100 mg/L is 2.4 d. According to the invention, the resource library of pesticide degrading bacteria is enriched, and the strain can rapidly and efficiently degrade acetochlor and is beneficial to repairing industrial sewage or farmland polluted by acetochlor. Due to the bioremediation characteristic of the strain, the strain can be used for research and development of complex microbial inoculants ...

Sulfonylurea herbicide hydrolase TsmE mutant as well as coding gene and application thereof

The invention discloses a sulfonylurea herbicide hydrolase TsmE mutant as well as a coding gene and application thereof, the mutant comprises 270th alanine mutated into arginine, and the amino acid sequence of the mutant is shown as SEQ ID NO.4. The invention further discloses a preparation method of the sulfonylurea herbicide hydrolase TsmE mutant. The specific enzyme activity of the mutant M4 (A270R) to the sulfonylurea herbicides is improved by 1.3-3.2 times compared with that of a wild type, so that the mutant M4 (A270R) has a good application prospect in the bioremediation of residual pollution of the sulfonylurea herbicides and the transgenic engineering of resisting the sulfonylurea herbicides.

Method for rapidly measuring and calculating cation exchange capacity of soil based on strontium chloride extraction agent

The invention discloses a method for rapidly measuring and calculating the cation exchange capacity of soil based on a strontium chloride extractant, which comprises the following steps: fully leaching soil by using a 0.01 M SrCl2 solution, measuring the concentration of metal cations in an exchange liquid by coupling inductively coupled plasma emission spectrometry (ICP-OES), and calculating the total quantity of the cations in the soil by summation, and substituting the measured total amount of cations into the linear equation to obtain the cation exchange capacity of the soil. The ratio of soil to liquid used in the method is only 1: 10, exchange can be completed twice, the operation process is simple and rapid, the precision is high, and the result reproducibility is good; in addition, the reagent dosage and variety in the test process are less, and the method is friendly to both experiment operators and environment. Therefore, the method for rapidly measuring and calculating the cation ...

Special fertilizer for nursery grown plant

Anaerobic degradation strain SRB-2 and application thereof

The invention discloses an anaerobic degradation strain SRB-2 and application thereof, the strain is Trichococcus sp.SRB-2, is a facultative anaerobic gram positive bacterium, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No.40302. The invention further discloses a preparation method of the anaerobic degradation strain SRB-2. Under an anaerobic condition or a micro-aerobic condition, the anaerobic degradation half-life period of the strain on metolachlor with the concentration of 20 mg/L is 2.0 d, and meanwhile, the strain shows a very good bioremediation effect on industrial sewage polluted by metolachlor. The strain enriches a resource library of pesticide degrading bacteria, has a wide prospect in the aspects of bioremediation of pollution of metolachlor or preparation of metolachlor anaerobic degrading bacteria and the like, and has a good application value in environmental protection.

Sulfonylurea herbicide hydrolase TsmE mutant as well as coding gene and application thereof

The invention discloses a sulfonylurea herbicide hydrolase TsmE mutant as well as a coding gene and application thereof, and a mutant M5 (A183S) of which the enzyme activity is remarkably improved is obtained by taking wild sulfonylurea herbicide hydrolase TsmE as a starting template through a directed evolution technology. Compared with a wild type, the specific enzyme activities of the mutant M5 on metsulfuron-methyl, metsulfuron-methyl, tribenuron-methyl, bensulfuron methyl and tribenuron-methyl are respectively improved by 1.0, 1.2, 3.6, 1.2 and 1.7 times. The mutant and the coding gene thereof can be used for constructing sulfonylurea herbicide resistant transgenic crops, can also be used for removing sulfonylurea herbicides in soil and water bodies, and have very important theoretical and application values.