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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 3. Отображено 3.
14-07-2023 дата публикации

In-vitro activation and amplification method of NK (Natural Killer) cells

Номер: CN116426475A
Автор: MA SHIQI, LIN JIAHUI, CHENG CHENG, ZHAN MINGGAO, CHEN XU, CHEN GANG
Принадлежит:

The invention relates to an in-vitro activation and amplification method of NK cells. The method specifically comprises the following steps: separating and extracting a mononuclear cell PBMC from human peripheral blood, and carrying out specific activation on the extracted mononuclear cell by adopting a nanoparticle suspension coupled with a biotin-labeled 2B4 antibody and a biotin-labeled IL-1beta antibody on the same day of NK cell activation culture, the NK cells with high purity, high amplification efficiency and high lethality can be obtained after 14 days of amplification culture. According to the present invention, with the application of the polypeptide in combination with the cell amplification factors IL-15 and IL-2, the purity and the amplification efficiency of the NK cells in the PBMC can be significantly improved, the amplified NK cells are in the good activated state, the purity of the NK cells can achieve more than 85% on the eighth day of culture, and the polypeptide has ...

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Номер записи: 1
18-04-2023 дата публикации

Hematopoietic stem cell serum-free medium and application thereof

Номер: CN115975926A
Автор: LIN JIAHUI, MA SHIQI, CHENG CHENG, ZHAN MINGGAO, CHEN XU, CHEN GANG
Принадлежит:

The invention relates to a hematopoietic stem cell serum-free culture medium and application thereof.The culture medium is prepared by mixing a basic culture medium and additive components, and the additive components comprise stem cell factors (SCF), recombinant human thrombopoietin (TPO), recombinant human Flt3 ligands (Flt3-L), rhIL-6, rhIL-3, CITED2, HSF1A, OAC1, recombinant human insulin, recombinant human apotransferrin, recombinant human serum albumin and ethanolamine. And sodium selenite. The basic culture medium is IMDM. The culture medium is clear in chemical component, free of serum, free of human-derived and animal-derived components, simple in formula and excellent in performance, the amplification capacity of the hematopoietic stem cells can be remarkably improved, the hematopoietic stem cells can be amplified by 500 times or above after being cultured for 13 days, the cell viability can reach 95% or above during harvesting, and the positive proportion of CD34 + cells is remarkably ...

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Номер записи: 2
29-08-2023 дата публикации

T cell serum-free culture medium and application thereof

Номер: CN116656607A
Автор: ZHAN MINGGAO, LIN JIAHUI, CHENG CHENG, MA SHIQI, CHEN XU, CHEN GANG
Принадлежит:

The invention relates to a T cell serum-free culture medium and application thereof. The culture medium comprises alkyl glucoside (APG) with the final concentration of 100-1000 mu g/mL and Tween-80 with the volume final concentration of 0.05-0.2%. PBMC is separated from human peripheral blood, APG and Tween-80 are added into a culture system for culture on the 0th day of T cell activation culture, the lentivirus transduction efficiency of T cells induced and activated from PBMC can be effectively improved, meanwhile, the amplification capacity and the active state of the T cells are not affected, and a powerful means is provided for obtaining more CAR-T cells in the clinical treatment process.

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Номер записи: 3