Intelligent terminal remote monitoring method and system

The invention provides an intelligent terminal remote monitoring method and system, and the method comprises the following steps: obtaining and executing a security policy from a monitoring server, the security policy being used for setting the application state of a software function and each hardware function module; acquiring and executing a behavior strategy from the monitoring server, wherein the behavior strategy is used for setting a permission state for executing each use behavior; acquiring operation state information of each hardware function module, and uploading the operation state information to a monitoring server; and judging whether the current use behavior violates the behavior strategy or not, if so, limiting the use of the software function and each hardware function module, and uploading behavior abnormal information to the monitoring server. According to the invention, remote monitoring of the card issuing equipment can be realized, and compliance use of the card issuing ...

Escherichia coli for producing L-valine as well as construction method and application of escherichia coli

The invention discloses escherichia coli for producing L-valine as well as a construction method and application of the escherichia coli. According to the present invention, after the ycgH gene in the escherichia coli is knocked out and ilvC is expressed, or the combination of the knockout of the yjiT gene and the expression of brnF and brnE, the knockout of the yjiV gene and the expression of ilvE and ilvD, the knockout of the trpR gene and the expression of ilvH mutant protein, and the knockout of lacI and lacZ genes and the expression of DNA polymerase is performed, the obtained recombinant bacterium can improve the L-valine yield, can be used for producing the L-valine, and has good application prospects.

Infrared image sea-sky-line self adaption detection method

The invention discloses an infrared image sea-sky-line self adaption detection method which includes the following steps that (1) an original infrared image Forg is obtained; (2) edge detection is conducted on the original infrared image through a Canny operator, and an edge image Fedge is obtained; (3) a sliding window model MWin, the sea-sky-line length and a model LEN sum are built; (4) full-figure traversal is conducted on the edge image Fedge through the sliding window model MWin, and the sum of lengths of sea-sky-lines of different lines is solved; (5) the length of the sea-sky-lines and the line with the maximum LEN sum are defined to be the positions of the current image sea-sky-lines; (6) smoothing is conducted on the current image sea-sky-lines, and the filter value Lineseasky of the p+1th frame of the sea-sky-lines is output.

Novel technique for extracting L-tryptophan from fermentation broth

The invention discloses a novel technique for extracting L-tryptophan from fermentation broth, which includes the following steps that: fermentation broth is pretreated, and the treated liquid is pumped into a ceramic microfiltration membrane filter, so that tryptophan microfiltrate without mycelium and mycoprotein are obtained; the tryptophan microfiltrate is pumped into an ultrafiltration membrane filter, so that tryptophan ultrafiltrate and concentrated liquid containing pigment and protein are obtained; the tryptophan ultrafiltrate is pumped into a nanofiltration membrane filter, so that concentrated tryptophan liquid and permeate containing salt are obtained; waste filtrate is comprehensively utilized to produce protein feed; industrial ethylic acid is used for promoting low-temperature crystallization, crystallization mother liquid is treated by the ion exchange method and used, and after crystals are centrifugally dehydrated and dried, the L-tryptophan product is obtained. The invention ...

Kiln top feeding device of double-hearth lime shaft kiln

The utility model discloses a kiln top feeding device of a double-hearth lime shaft kiln, belongs to the technical field of lime shaft kiln feeding equipment, and is used for double-hearth feeding. The technical scheme is as follows: the distribution hopper is fixedly connected with the bracket; a top cover is arranged at an upper opening of the distribution hopper; a rotary chute inlet hole is formed in the top cover, the rotary chute driving device is fixed to the upper portion of the support and connected with the rotary chute, the upper end of the rotary chute is rotationally connected with the lower end of the reversible adhesive tape machine head cover, and the lower portion of the rotary chute extends into the distribution hopper through the rotary chute inlet hole in the top coverof the distribution hopper; a rotary chute inlet hole of the distributing hopper top cover is located in the center of the distributing hopper top cover, the lower portion of the rotary chute is bentoutwards ...

Power grid visualization method based on plugins

The invention discloses a power grid visualization method based on plugins. The method comprises the following steps that a power grid visualization system is started, and a primitive plugin and a visualization special effect plugin register to the power grid visualization system; primitive instances of the primitive plugin are initialized, and primitive files are generated; according to the primitive files, the primitive instances are constructed, and all the primitive instances register to a primitive list; after the primitive list registers, a visualization special effect thread and a primitive drawing thread are triggered; the primitive drawing thread draws primitives in the primitive list, and visualization special effects are overlapped by the visualization special effect thread according to the primitives sequentially arranged in the primitive list. A unified standard interface is adopted by the primitive plugin and the visualization special effect plugin, the plugins conforming ...

Escherichia coli flagellum specific ATP synthetase gene fliI and application of mutant thereof in production of L-amino acid

The invention discloses an Escherichia coli flagellum specific ATP synthetase gene fliI and application of a mutant of the Escherichia coli flagellum specific ATP synthetase gene fliI in production of L-amino acid. The sequence of the gene fliI disclosed by the invention is SEQ ID No.1, a protein shown as SEQ ID No.2 is encoded, the sequence of a fliI mutant is SEQ ID No.4, and a protein shown as SEQ ID No.5 is encoded. Experiments prove that the gene fliI, the mutant thereof and related biological materials can improve the yield of L-amino acids, especially L-threonine, L-tryptophan, L-arginine and L-valine, can be used for producing the L-amino acids, and has a very good application prospect.

Method for identifying internet traffic of mobile terminal with mobile phone number in real time based on signaling association

The invention provides a method for identifying network traffic of a mobile terminal with mobile phone numbers in real time based on signaling association, which comprises the following steps of: establishing a full-quantity mobile phone number association relationship library for storing signaling association data received in real time; establishing a mobile phone number tracking state table; receiving a to-be-identified mobile phone number, taking the to-be-identified mobile phone number as an index, searching corresponding association relationship data in the full-amount mobile phone number association relationship library, and updating the corresponding association relationship data to a mobile phone number tracking state table; setting updated information as a unique feature of Internet traffic identification, and triggering a traffic analysis device to issue a feature rule of a current to-be-identified mobile phone number to a high-speed traffic matching engine according to the unique ...

Engineering bacterium for producing L-valine as well as construction method and application of engineering bacterium

The invention discloses an engineering bacterium for producing L-valine as well as a construction method and application thereof. It is found that after a yjiT gene in escherichia coli is knocked out and brnF and brnE are expressed, or a yjiV gene is knocked out and ilvE and ilvD are expressed, a trpR gene is knocked out and ilvH mutant protein is expressed, lacI and lacZ genes are knocked out and DNA polymerase is expressed, and a ycgH gene is knocked out and ilvC is expressed, the obtained recombinant bacterium can increase the yield of L-valine, can be used for producing L-valine and has a good application prospect.

Fast speed adjusting in broadband is receiver frequently based on DDS

The utility model discloses a quick frequency hopping receiver in broadband based on DDS, including a single -pole double -throw, two preamplification wave filters, the 2nd single -pole double -throw, intermediate frequency processing passageway, adc and the main control chip that connect gradually, main control chip controls a single -pole double -throw and the 2nd single -pole double -throw respectively to connect the 3rd single -pole double -throw through variable local oscillator module, the 3rd single -pole double -throw connects in parallel at the output of individual preamplification wave filter in the 2nd single -pole double -throw. The utility model discloses based on in DDS's the quick frequency hopping receiver, adopt DDS to obtain the required high frequency local oscillator of mixing as low frequency variable frequency source, the mode of utilizing the frequency multiplier to add filtering.

Hydraulic braking controlling means

The utility model relates to a hydraulic braking controlling means, include: the system is establish to pressure, pressure is establish the system and is establish high -pressure state to hydraulic oil, the energy storage system, the energy storage system with pressure is establish the system and is directly or indirectly linked together, the system is establish through being in to pressure the high pressure state hydraulic oil transmits to the energy storage of energy storage system implementation hydraulic pressure, the pressure control system, the pressure control system is through control track transportation vehicles's braking system is controlled to pressure in the energy storage system, and protection system, protection system with pressure establish the system with the energy storage system connects in parallel and/or establishes ties together, with the protection pressure establish the system with the energy storage system normally works. Therefore, the utility model provides a ...

Amino acid yield related protein coding gene pyrG and related strain, biological material and application thereof

The invention discloses an amino acid yield related protein pyrG as well as a biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the problem of regulating and controlling the yield of microbial amino acids, wherein the amino acids specifically can be L-threonine, L-tryptophan, L-arginine and L-valine. The protein is any one of the following proteins: A1) a protein of which the amino acid sequence is obtained by replacing the 450th aspartic acid in a sequence 2 with a sequence shown as histidine, serine, glycine, alanine, valine or phenylalanine; a2) a protein which is obtained by substitution and/or deletion and/or addition of amino acid residues on the amino acid sequence of A1), has 80% or more of identity with the protein shown in A1) and has the function of regulating and controlling the yield of microbial amino acid; and A3) a fusion protein which is obtained by connecting a tag to the N end and/or C end of A1) or ...

METHOD FOR PRODUCING L-LYSINE BY MODIFYING ACONITASE GENE AND/OR REGULATORY ELEMENTS THEREOF

Provided is an L-lysine generation method by fermentation, comprising modifying the aconitase gene and/or regulatory element thereof on a bacteria chromosome, such that the enzymatic activity and/or expression quantity of the aconitase are reduced but do not disappear; and generating L-lysine by fermenting the modified bacteria. Also disclosed are methods and uses derived from the present method, and the bacteria that can be used in these methods and uses.

Parking braking control system for railway vehicles

The invention provides a parking braking control system for railway vehicles. The parking braking control system comprises a parking cylinder and a double-pulse solenoid valve or a two-position three-way solenoid valve; a compression spring is arranged in the parking cylinder; one end of the compression spring is connected with the bottom of the parking cylinder and the other end of the compression spring is connected with a piston in the parking cylinder; the output end of the double-pulse solenoid valve or the two-position three-way solenoid valve is connected with the parking cylinder, and the input end of the double-pulse solenoid valve or the two-position three-way solenoid valve is connected with a wind source; when a vehicle runs, the wind source enters the parking cylinder through the double-pulse solenoid valve or the two-position three-way solenoid valve to compress the compression spring; when the vehicle is needed to be parked for a long time, the double-pulse solenoid valve ...

Slag dryer control system, control method and motor controller

The invention provides a slag drying machine control system, a control method and a motor controller, and relates to the technical field of boiler dry type slag discharging, the slag drying machine control system comprises a plurality of weighing rollers arranged on the surface of the side, away from ash receiving, of a conveying belt, and the motor controller electrically connected with the weighing rollers and a motor; wherein the weighing rollers are used for generating corresponding gravity signals based on the gravity of ash residues at the corresponding positions of the conveying belt and sending the gravity signals to the motor controller; and the motor controller is used for controlling the running speed of the motor according to the plurality of gravity signals. The working efficiency of the slag dryer can be improved, so that smooth slag discharge of the boiler is facilitated, and normal operation of the boiler is further facilitated.

Clutter map partitioning method based on image processing

The invention discloses a clutter map partitioning method based on imaging processing. The clutter map partitioning method includes steps of (1) calculating radar operating range and beam width and determining clutter map partitioning modes and resolution cells; (2) counting clutter signal strength in a clutter map unit and determining iteration coefficient; (3) subjecting clutter map data to median filtering and eliminating abnormal points having overlarge or too small estimated values by the median filtering; (4) partitioning a clutter map; (5) expanding a clutter map detecting unit, to be specific, simultaneously judging other eight units adjacent to one another in distance and direction when the clutter strength of the clutter unit is estimated, and finally subjecting the judgment results of the nine units to greatest selection processing as the final estimated value of the clutter scene; (6) detecting the clutter map, to be specific, expanding information by the clutter map detecting ...

Radar data moving-target detection processing method based on tracking filter

The invention discloses a radar data moving-target detection processing method based on a tracking filter. The method comprises the following steps: (1) conducting signal preprocessing on a pulse pressure signal; (2) filtering the signal obtained in the step (1), wherein filtration is divided into a detection state and a tracking state, in the detection state, M MTD filters are adopted to output target signals of M Doppler frequency channels, and in the tracking state, a tracking filter is adopted for filtering specified Doppler frequency signals; (3) conducting clutter map processing on output data in the step (2); (4) conducting constant false-alarm processing on a result of the step (3); (5) analyzing and calculating the clutter property, classifying clusters, adopting detecting thresholds in various corresponding clutter areas, and conducting target detection; (6) synchronizing detection signals of the same distance element of various channels to obtain a target detection result of ...

Microbial bacteriostatic agent for oil field and preparation method thereof

The invention relates to the technical field of petroleum assistants for oil field oil extraction and sewage treatment, and discloses a microbial bacteriostatic agent for an oil field and a preparation method thereof, the preparation method comprises the following six steps: (1) obtaining a preliminary screening strain: separating and storing the strain which grows rapidly in produced water and enables the SRB concentration to be greatly reduced as the preliminary screening strain; (2) obtaining dominant strains; (3) culturing and separating dominant strains; (4) selecting brevibacillus brevis and candida vesii; (5) obtaining a mixed bacterial agent; and (6) obtaining the mixed microbial bacteriostatic agent. The microbial bacteriostatic agent prepared by the preparation method disclosed by the invention can have a rapid and lasting bacteriostatic effect on harmful SRB bacteria under a low-concentration condition, is non-toxic to people, livestock and fishes, and does not cause pollution ...

Directional transformation method of tyrosine production strain, production strain and tyrosine fermentation method

According to the directional transformation method of the tyrosine production strain, the tyrosine production strain and the tyrosine fermentation method, the expression intensity of aroD, aroA, aroB and aroK genes is adjusted on the basis of an original strain E.coli TR03, and the tyrosine production strain obtained through shikimic acid pathway transformation has the advantages of being high in acid production rate and short in fermentation period; when the high-pressure fermentation method of the tyrosine production strain is used for high-pressure fermentation, the tyrosine production intensity can be effectively improved, the water and electricity consumption can be greatly reduced in production, the water and electricity cost and the labor cost of single fermentation can be greatly saved, and the tyrosine synthesis rate can be effectively improved; the production strain can reach the maximum product concentration in a short time, the fermentation batch is increased, and the production ...

Acetolactate synthase III regulatory subunit coding gene ilvH, mutant thereof and application of coding gene ilvH in production of L-amino acid

The invention discloses an acetolactate synthase III regulatory subunit coding gene ilvH, a mutant thereof and application of the acetolactate synthase III regulatory subunit coding gene ilvH in production of L-amino acid. The invention provides an ilvH mutant protein or a fusion protein containing the ilvH mutant protein. The ilvH mutant protein is a protein obtained by mutating the 47th amino acid residue of an acetolactate synthase III regulation subunit ilvH protein; experiments prove that in corynebacterium glutamicum, the yield of L-isoleucine can be increased by over-expression and point mutation of the corynebacterium glutamicum acetolactate synthase III regulation subunit coding gene ilvH; meanwhile, overexpression and point mutation of the ilvH gene in Escherichia coli can improve the yield of L-valine in Escherichia coli.

Steam curing trolley hook assembly and steam curing trolley using same

The invention relates to the field of article transfer tools, in particular to a steam curing trolley hook assembly and a steam curing trolley using the hook assembly. The steam curing trolley hook assembly comprises a hook. A crook part is arranged at the front end of the hook. The front side face of the crook part is a slope. A hinge hole is formed in the rear end of the hook and used for being hinged with the other trolley to enable the front end to do pitching motion. A trundle is assembled between the two ends of the hook. The axis of the trundle and the axis of the hinge hole are parallel to each other. In the using process, when two trolleys need to be connected, the rear trolley just needs to be pushed to make the slope on the front side of the crook part of the hook make contact with a hinge pin on the front trolley, and the hook pitches up under the matching effect of the hinge pin and the slope on the front side of the crook part to be connected with a hook of the front trolley ...

Gear transmission device

The utility model discloses a gear transmission device, including spacing mounting panel and rotating electrical machines, be equipped with the mounting panel and the motor mounting panel of the control panel of relative position detection on the spacing mounting panel, it is provided with the rotation axis of being connected with gear drive to run through between control panel mounting panel andthe motor mounting panel, straight -tooth conical gear has been cup jointed on the rotation axis, the output of rotating electrical machines realizes through tapered gear and straight -tooth conical gear meshing transmission that the right angle turns to the transmission. The utility model discloses a gear transmission device has simple structure, transmission stability is good, the commonality isstrong, the maintenance is convenient and the security is good characteristics.

Production method for extracting tryptophan from fermentation liquor by one-step refining

The invention discloses a production method for extracting tryptophan from fermentation liquor by one-step refining, which comprises the pretreatment of the fermentation liquor. In the method, mycoprotein is filtered off by using a ceramic microfiltration membrane, is decolorized by using an ultrafiltration membrane and is desalted and concentrated by using a nanofiltration membrane; the nanofiltration membrane concentrated liquor is directly decolorized by using activated carbon, the decolorized liquor is subjected to isoelectric point crystallization at a low temperature, and a qualified competitive product is obtained by rinsing with ice water during centrifugal separation; the crystallization mother liquor returns to the nanofiltration membrane for circulation; protein feed with high additional value is prepared by mixing, drying and crushing the ultrafiltration strong liquor and mycoprotein; and nanofiltration strong brine can be used as a culture medium for later use or sold serving ...

Novel technique for extracting L-tryptophan from fermentation broth

The invention discloses a novel technique for extracting L-tryptophan from fermentation broth, which includes the following steps that: fermentation broth is pretreated, and the treated liquid is pumped into a ceramic microfiltration membrane filter, so that tryptophan microfiltrate without mycelium and mycoprotein are obtained; the tryptophan microfiltrate is pumped into an ultrafiltration membrane filter, so that tryptophan ultrafiltrate and concentrated liquid containing pigment and protein are obtained; the tryptophan ultrafiltrate is pumped into a nanofiltration membrane filter, so that concentrated tryptophan liquid and permeate containing salt are obtained; waste filtrate is comprehensively utilized to produce protein feed; industrial ethylic acid is used for promoting low-temperature crystallization, crystallization mother liquid is treated by the ion exchange method and used, and after crystals are centrifugally dehydrated and dried, the L-tryptophan product is obtained. The invention ...

Using method for creating BIM model through laser scanning of existing building

The invention discloses a using method for creating a BIM model through laser scanning of an existing building, and the method is used for creating a digital building model and position sharing for the existing building, and the position in the building is determined by referring to official surveying and mapping points outside the building and serves as a reference floor of the building. Applying a machine readable mark in the creation family library at the specified position; wherein a mark in the reference original model is read through properly equipped mobile laser scanning equipment, so that data restoration is performed on the geometrical shape of the reference original model based on the position of the read mark; creating a digital volume model for a room in a reference floor with an appropriate symbol; when the digital volume model is created for basically the same floor of the building, the digital volume model of the reference floor is used as the reference volume model, and ...

Roller changing method for finishing mill

The invention provides a finishing mill roll changing method which comprises a rack preparation time sequence, an upper roll falling time sequence, an old roll extracting time sequence and a new roll pushing time sequence, specifically, when finishing mill roll changing is carried out, the rack preparation time sequence is carried out firstly, the upper roll falling time sequence is carried out after the rack preparation time sequence is completed, and the old roll extracting time sequence is carried out after the upper roll falling time sequence is completed; and after the old roller extraction time sequence is finished, a new roller pushing time sequence is carried out, and after the new roller pushing time sequence is finished, roller replacement of the finishing mill is completed. According to the method, the problem that the time used in the roll changing process of the finishing mill is too long is solved, the conventional steps during roll changing of the finishing mill are simplified ...

Formwork supporting structure convenient to mount and dismount

The invention discloses a formwork supporting structure convenient to mount and dismount. Mounting clamping grooves are symmetrically and fixedly formed in the two sides of a supporting plate. Connecting protruding plates are symmetrically and fixedly arranged on the two sides of the mounting base and are symmetrically fixed to a building wall through screws. Through cooperative use of the arranged connecting and fixing piece and the pressing piece, the supporting plate can be quickly mounted, the tightness and stability of connection between the supporting plate and the building wall can be improved through the pressing piece, and later dismounting of the supporting plate and the building wall is also very convenient; the mounting base and the connecting fixing piece are used in cooperation with the pressing piece, the mounting strength of the supporting plate is guaranteed, meanwhile, corresponding damage to a building wall is avoided, the construction quality of the supporting plate ...

Agricultural deep water-saving system for groundwater irrigation of Mauwu sand

The invention discloses an agricultural deep water-saving system for groundwater irrigation of Gou Uu sand, which comprises a plurality of groups of temperature monitoring units, each temperature monitoring unit comprises a plurality of temperature sensors, and the temperature sensors in each temperature monitoring unit are uniformly distributed in the longitudinal direction; each humidity monitoring unit comprises a plurality of humidity sensors, and the humidity sensors in each humidity monitoring unit are uniformly distributed in the longitudinal direction; the temperature sensor and the humidity sensor are connected in parallel; a temperature monitoring unit and a humidity monitoring unit are distributed on the periphery of each planting pit; water vapor in the planting pits is prevented from leaking out through the protective film, irrigation water resources are prevented from being rapidly evaporated in a special environment, the utilization rate of the water resources is increased ...

Industrial salt wastewater treatment equipment

The invention belongs to the field of industrial salt wastewater treatment, and particularly relates to industrial salt wastewater treatment equipment which comprises a crystallization barrel and is characterized in that an incineration box is fixedly arranged at the bottom of the crystallization barrel, and a bin door capable of being opened and closed is arranged at one end of the incineration box in the axis direction of the crystallization barrel; a collecting barrel for collecting solid crystals is rotatably arranged in the crystallization barrel, an arc-shaped baffle is fixedly arranged in the collecting barrel, a rotating shaft is rotatably arranged in the collecting barrel, a plurality of crystallization plates are uniformly distributed on the outer circumferential surface of the rotating shaft, and a plurality of meshes for accelerating evaporative crystallization of wastewater are formed in the surfaces of the crystallization plates. A gas washing barrel is fixedly arranged on ...

Feeder management system

A feeder management system is used for managing the feeder of a surface mount device, which comprises a storage card fixed in the feeder and can save the related information of the feeder, and a console electrically connected with the storage card and can read the storage information of the storage card, the console controls a machine desk of the surface mount device to pick up the need electronic elements in the feeder according to the storage information of the storage card. Because the feeder management system adopts a storage card to save the related information of the corresponding feeder, the feeder management system not only can save more information about the feeder, but also rewrite the related information according to the current status of the feeder, even improves the confidentiality of the related information by encrypting, which is convenient to manage the feeder.

Recombinant bacterium and application thereof in production of L-valine

The invention discloses a recombinant bacterium and an application of the recombinant bacterium in production of L-valine. The recombinant bacterium is obtained by knocking out an ilvE (E) gene and simultaneously inserting a ptrc-started ilvE (B) gene from bacillus subtilis in escherichia coli for producing valine. The engineering bacterium is obtained by'knockout of a thiE gene and knockout of an ilvE (E) gene and insertion of a ptrc-started ilvE (B) gene from bacillus subtilis' or'knockout of a thiE gene and an mdh gene and knockout of an ilvE (E) gene and insertion of a ptrc-started ilvE (B) gene from bacillus subtilis'. The yield of L-valine can be increased by fermenting the recombinant bacteria. The method has an important application value.

Adjustable vegetation irrigation machine

The invention discloses an adjustable vegetation irrigation machine, and relates to the technical field of vegetation irrigation, the adjustable vegetation irrigation machine comprises two bases, the bases are provided with displacement mechanisms, the displacement mechanisms are provided with insertion thorns and moving wheel mechanisms, the bases are fixedly connected with vertical rods, the ends, away from the bases, of the vertical rods are fixedly connected with a top plate, and the top plate is provided with a width adjusting mechanism. A height adjusting block mechanism is arranged on the vertical rod and the top plate, a centering mechanism is arranged on the top plate, a spraying irrigation mechanism is arranged between the height adjusting block mechanism and the centering mechanism, and a water supply mechanism is arranged between the base and the spraying irrigation mechanism. Inserting thorn positioning can be achieved through inserting thorns, moving of the whole device can ...

Portable ADS-B mobile system with 3g function

The invention discloses a portable ADS-B mobile system with 3G function, and the system comprises a signal data processing module, an ADS-B sending-receiving module, a 3G sending-receiving module, GNSS receiving module and a display module; the signal data processing module is used for processing the signal of the GNSS receiving module, 3G sending-receiving module and ADS-B sending-receiving module, extracting the information and filtering, unpacking and packing; the ADS-B sending-receiving module is used for receiving the ADS-B information of radio broadcast and transforming the ADS-B information packed by the signal data processing module into the radio frequency signal for sending; the 3G sending-receiving module is used for receiving the target information and flying service information sent by the ground server and sending the self-location information; the GNSS receiving module is used for receiving the signal from the GNSS navigation system and sending the data to the signal data ...

Effect of isocitrate lyase gene aceA on synthesis of L-amino acid

The invention discloses an effect of an isocitrate lyase gene aceA on synthesis of L-amino acid. The invention specifically discloses an application of a protein with an amino acid sequence of SEQ ID No.2 or a substance for regulating and controlling the activity and/or content of the protein in construction of genetically engineered bacteria for producing L-amino acid. According to the invention, an aceA gene mutant engineering strain obtained by introducing point mutation (C-T) to the 1111th site of an aceA gene coding region of an L-amino acid high-yield strain and wild escherichia coli W3110, and an engineering strain obtained by knocking out an aceA gene are constructed. Experiments show that the aceA gene and a variant aceAQ371 * gene thereof participate in biosynthesis of L-amino acid, and accumulation of the L-amino acid is facilitated by knocking out or weakening the aceA gene. The aceA gene and the variant thereof can be used for constructing a genetic engineering strain for producing ...

It receives face instrument to be used for hanging mould bottom concrete

The utility model discloses an it receives face instrument to be used for hanging mould bottom concrete, including trowel main part and sliding ruler, the last fixed surface of trowel main part installs two slip round bars, the fixed scale that is provided with of upper surface opposite side of trowel main part, sliding ruler includes two connecting rods and control panel, and the equal fixed connection in rear end of two connecting rods is in one side of control panel, the one end of sliding block all runs through and is provided with the elasticity screw, and in the head end of elasticity screw all extends to sliding clamping groove, and two sliding clamping groove block respectively and establish on two slip round bars, the control panel is located the top of scale. This it receives face instrument to be used for hanging mould bottom concrete to can adjust at will through scale and sliding ruler according to the on -the -spot condition and obtain required drilling depth size, avoidthe ...

Grape vine burring machine

The invention discloses a grape vine burring machine. A power input shaft is arranged on the upper part of a machine frame; a driving chain wheel is fixed to the tail end of the power input shaft; a power output shaft is arranged on the lower part of one side of the machine frame; a driven chain wheel is fixed to the tail end of the power output shaft; an adjustable depth wheel is fixed to the other side of the machine frame through an adjustable support; a plurality of scarification blades are fixed on the power output shaft; a soil collecting hood is arranged on the outer sides of rotating circles of the scarification blades; a soil loosening ploughshare is arranged at the front end of the power output shaft; an arc-shaped soil diversion hood is also arranged on the upper part of the machine frame; and the lower end of the soil diversion hood is communicated with the upper end of the soil collecting hood. The grape vine burring machine has the advantages of labor conservation and high ...

Poor charge level indicator of gravity

The utility model discloses a poor charge level indicator of gravity, including installing two sets of fixed pulleys on the feed bin roof, installing rope on two sets of fixed pulleys, installing one set at least proximity switch on the feed bin lateral wall and fix inside weight and the outside weight at the rope both ends respectively, two sets of fixed pulley levels are laid, and one of them cover is located feed bin roof middle part, and other one set is located limit department outside the feed bin roof, and inside weight and outside weight are located the feed bin inboard and the outside respectively, the utility model discloses for current charge level indicator, its environmental suitability is stronger, long service life, and the structure is simpler moreover, and the level check is accurate, is applicable to the level check of various graininess materials.

Infrared image target detection method based on histogram modification

The invention discloses an infrared image target detection method based on histogram modification. The method includes the following steps: (1) calculating a gradient image phi(x,y) based on an original image Forg; (2) performing non-maximum suppression on phi(x,y) to obtain an image phi1(x,y); (3) conducting edge connection by using the image phi1(x,y) to obtain a candidate target area Obj0; (4) obtaining the histogram Histo of Obj0; (5) setting the height proportionality coefficient of the Histo and calculating the high threshold Thigh and the low threshold Tlow; (6) modifying the Obj0 according to Thigh and Tlow to obtain Obj1; (7) determining the edge closure of Obj1 and calculating the target position Pos(x,y).

Tkt gene mutant and application thereof in preparation of L-lysine

The invention discloses a tkt gene mutant and application of the tkt gene mutant in preparation of L-lysine. The tkt gene mutant disclosed by the invention is DNA (deoxyribonucleic acid) molecules as shown in SEQ ID No.3, 5, 7, 9, 11 and 13 in a sequence table and is used for coding proteins as shown in SEQ ID No.4, 6, 8, 10, 12 and 14, and the wild type tkt gene is a DNA molecule as shown in SEQ ID No.1 and is used for coding a protein as shown in SEQ ID No.2. Experiments prove that the tkt gene and the mutant thereof can improve the yield of L-lysine, can be used for producing L-lysine, and have a very good application prospect.

Disc type brake gripping mechanism for railway vehicles

The invention discloses a disc type brake gripping mechanism for railway vehicles. The mechanism is invented for dissolving the problems of unstable brake force, poor brake effect and the like in the prior art. The mechanism comprises a brake rod mechanism which is formed by two opposite brake rods and a suspension arm, wherein the two ends of the suspension arm are movably connected with the two brake rods so that the two brake rods rotate in the same plane by taking the two ends of the suspension arm as supporting points; the two ends of the two brake rods at one side of the suspension arm are correspondingly connected with brake lining supports; the two ends of the two brake rods at the other side of the suspension arm are correspondingly connected with brake cylinders; and the middle part of the suspension arm is suspended on a bogie of a locomotive. According to the structure, two brake lining supports rotate around respective supporting point with the two brake rods and relatively ...

Antiskid blow down valve for braking system of high-speed train

The invention relates to an antiskid blow down valve for a braking system of a high-speed train. The antiskid blow down valve comprises a valve base body, wherein an air inlet, an air outlet and an exhaust port are formed on the valve base body; the communication between the air inlet and the air outlet and the communication between the air outlet and the exhaust port are achieved through dynamic switch passages. The antiskid blow down valve has a small size and a simpler structure, is convenient for installation during use, and is easy to maintain and repair.

Be used for reinforced (rfd) reinforcement instrument of oblique cylindrical template

The utility model discloses a be used for reinforced (rfd) reinforcement instrument of oblique cylindrical template, including first vaulting pole, second vaulting pole, third vaulting pole and fourthvaulting pole, the equal fixedly connected with base of lower extreme of first vaulting pole, second vaulting pole, third vaulting pole and fourth vaulting pole runs through on the sill bar and has seted up a plurality of screw eye, and spiral shell rasp bar's the equal fixedly connected with in upper end holds in the palm on the top, and the rotation groove has all been seted up to the upper endthat held in the palm on the top, rotate the inslot all the activity be provided with a rotating head, all run through being provided with fixing bolt II on the both sides that held in the palm on the top, fixing bolt II's front end all extends to the rotation inslot, and the upper end of rotating head is all through connecting rod fixedly connected with layer board. This be used for reinforced (rfd ...

L-tyrosine yield related protein yccE as well as biological material and application thereof

The invention discloses an L-tyrosine yield related protein yccE as well as a biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the problem of regulating and controlling the yield of microorganism L-tyrosine. The protein is any one of the following proteins: A1) a protein, the protein is a yccE protein or the 415th arginine residue of the amino acid sequence of the yccE protein is replaced by any one of the following substances: phenylalanine residue, leucine residue, isoleucine, methionine residue, valine residue, serine residue, proline residue, threonine residue, alanine residue, tyrosine residue, histidine residue and glutamine residue, and the amino acid sequence of the yccE protein is represented by the formula I. The amino acid sequence of the yccE protein is represented by the formula I. The amino acid sequence of the yccE protein is represented by the formula I. The amino acid sequence of the yccE protein is ...

A method of decoding common-frequency overlapping of 1090 ES signals based on secondary inflexion-point method

In a method of decoding common-frequency overlapping of 1090 ES signals based on secondary inflexion-point method of the present invention, a video signal relevant accumulation is adopted, and two rounds of inflexion-point detection is performed, to extract a secondary inflexion-point and determine a starting time and a terminating time of double overlapped pulses, so as to achieve the function of correctly decoding under the circumstance of 1090 ES signals overlapping. Since it is hard for the common-frequency overlapped pulses to be separated from the angle of the signals, the present invention considers from the time domain feature of the overlapped signals of an envelope, and recovers the frame of the original signals by determining the starting time and the terminating time of the two overlapped pulses. The present invention can technically improve the current 1090 ES ground monitoring system, achieve correct decoding under the circumstance of 1090 ES signals overlapping, and increasing ...

Method for fermentation-production of pentanediamine comprising carbon dioxide stripping technique

A method for fermentation-production of a pentanediamine, comprising: culturing a cell expressing a lysine decarboxylase to obtain a whole cell fermentation broth comprising a pentanediamine; and extracting the pentanediamine from the whole cell fermentation broth, and striping the whole cell fermentation broth of carbon dioxide contained therein before adding a strong base. The method greatly increases a production volume of the pentanediamine.

Grape vine burring machine

The invention discloses a grape vine burring machine. A power input shaft is arranged on the upper part of a machine frame; a driving chain wheel is fixed to the tail end of the power input shaft; a power output shaft is arranged on the lower part of one side of the machine frame; a driven chain wheel is fixed to the tail end of the power output shaft; an adjustable depth wheel is fixed to the other side of the machine frame through an adjustable support; a plurality of scarification blades are fixed on the power output shaft; a soil collecting hood is arranged on the outer sides of rotating circles of the scarification blades; a soil loosening ploughshare is arranged at the front end of the power output shaft; an arc-shaped soil diversion hood is also arranged on the upper part of the machine frame; and the lower end of the soil diversion hood is communicated with the upper end of the soil collecting hood. The grape vine burring machine has the advantages of labor conservation and high ...

Power grid integrated information remote access method

The invention discloses a power grid integrated information remote access method. The method includes allowing a local control center to transmit a power grid integrated information remote access service request to a remote control center, allowing the remote control center to make decisions according to the remote access service request and return image files to the local control center, and allowing the local control center to analyze the image files and transmit an image data service request to the remote control center; allowing the remote control center to query real-time data indexes in a power grid integrated information database according to the content of the image data service request, acquiring the power grid integrated information data according to the indexes, and transmitting the power grid integrated information data to the local control center after power grid integrated information combination; allowing the local control center to receive and display the power grid integrated ...

YejG gene, mutant thereof and application of YejG gene and mutant thereof in preparation of amino acid

The invention discloses a eejG gene, a mutant of the eejG gene and application of the eejG gene and the mutant in preparation of amino acid. The invention provides a ycjGW96 protein (obtained by mutating a 96th amino acid residue of the ycjG protein from W to other amino acid residues) or a fusion protein containing the ycjGW96 protein, and also provides a related biological material. Application of the ycjGW96 protein, the fusion protein or the biological material: application in improving the amino acid yield of microorganisms or cells; application in the production of amino acids; the invention also relates to application in construction of recombinant microorganisms or recombinant cells for producing amino acids. According to the invention, multiple mutation forms of ycjGW96 protein are obtained through point mutation, and the function of the ycjGW96 protein is superior to that of the ycjG protein. According to the invention, the ycjGW96L protein with the optimal function is obtained ...

Infrared degraded image adaptive restoration method

The invention relates to an infrared degraded image adaptive restoration method. The infrared degraded image adaptive restoration method comprises the following steps that: (1) an input image I [in] is obtained; (2) an image degradation model A is constructed; (3) the information entropy of the image I [in] is calculated; (4) a regularization coefficient Lambda is calculated according to H; (5) an image I can be restored through iterative operation; and (6) an restored image I [out] is outputted. Compared with the prior art, the infrared degraded image adaptive restoration method of the invention has the following advantages that: (1) the infrared degraded image adaptive restoration method is especially designed for low signal-to-noise-ratio infrared images, and detail information can be effectively restored when noise is suppressed; (2) the regularization parameter can be calculated adaptively according to the information entropy of local regions of the image, and the infrared degraded ...

Water treatment machine for pond floating garbage

The invention relates to the technical field of water garbage treatment, and discloses a pond floating garbage water treatment machine which comprises a floating plate, a garbage collecting mechanism and a walking mechanism. The garbage collecting mechanism comprises an opening-shaped receding groove formed in one end of the floating plate, supporting bases inclined to the floating plate are arranged on the two sides of the receding groove, and the two ends of each supporting base are rotationally connected with conveying wheels; the walking mechanism comprises a first supporting plate arranged at the end, close to the garbage collecting mechanism, of the floating plate, walking wheels are rotationally connected to the end of the first supporting plate, a second supporting plate is arranged in the middle of the floating plate, and propellers rotating synchronously with the walking wheels are arranged on the side face of the second supporting plate. The end, away from the garbage collecting ...

Double-inclined-bridge feeding device of double-hearth lime shaft kiln

The utility model discloses a double-inclined-bridge feeding device of a double-hearth lime shaft kiln, which belongs to the technical field of lime kiln equipment and is used for simultaneously feeding different materials and same materials with different granularities. The technical scheme is as follows: the two skew bridges are arranged in parallel; kiln front weighing hoppers are respectivelyarranged at the lower parts of the two skew bridges; a kiln top weighing hopper and a vibrating feeder are respectively arranged at the upper ends of the two skew bridges; a reversible sealing-tape machine is arranged below the two vibrating feeders; two rotary hoppers are arranged below the reversible sealing-tape machine, the two rotary hoppers are opposite to the two kiln chambers respectively,the winch is of a double-roller structure, the rotating directions of two rollers of the winch are opposite, steel wire ropes on the two rollers are connected with the two single-hopper trolleys respectively ...

Construction method and application of escherichia coli for producing L-valine

The invention discloses a construction method and application of escherichia coli for producing L-valine. According to the invention, after a trpR gene in escherichia coli is knocked out and ilvH is expressed, or lacI and lacZ genes are knocked out and DNA polymerase is expressed, and a ycgH gene is knocked out and ilvC is expressed, the obtained recombinant bacterium can improve the yield of L-valine, can be used for producing L-valine, and has a very good application prospect.

Intelligent fuel system of thermal power plant

The invention discloses an intelligent fuel system of a thermal power plant. The system comprises an as-fired coal quality analysis module, a coal full-process monitoring module, a fuel value management module and a matching blending combustion module, the in-furnace coal quality analysis module is used for analyzing the internal content of the coal before entering the furnace and calculating the amount of other elements in the coal; the coal full-flow monitoring module is used for carrying out online monitoring and mutual correction on a coal feeding belt, a coal plough, a material level and a coal feeding device; the fuel value management module is used for calculating the optimal coal blending combustion proportion and performing comprehensive calculation in combination with factors influencing coal combustion; the matching and blending combustion module is used for performing coal quality data comparison after the fuel value management module calculates the optimal coal blending combustion ...

Parking brake cylinder for railway vehicles

The invention provides a parking brake cylinder for railway vehicles. The parking brake cylinder comprises a concave parking cylinder cover and a parking intermediate body bucked above the parking cylinder cover, the parking cylinder cover and the parking intermediate body enclose to form a parking driving cavity, and an intermediate body is connected to the upper side of the parking intermediate body; the parking brake cylinder further comprises a driving mechanism arranged in the parking driving cavity; a concave surface is arranged in the upper portion of a switching mechanism and is arranged in the intermediate body, and the lower portion of the switching mechanism extends into the parking driving cylinder and is separately connected with the driving mechanism; the switching mechanism can be driven to move vertically through the driving mechanism; an executing mechanism is slidably abutted against the concave surface, and the switching mechanism pushes the executing mechanism to move ...

Low-speed target clutter map detection method

The invention relates to a low-speed target clutter map detection method. The method comprises the steps that equal sector division is carried out according to the radar action range to determine clutter map units obtained through division; the intensity of clutter signals in the clutter map units is calculated, and clutter map estimation is carried out on a single scanning frame; iteration is carried out on the clutter statistical average of the same clutter map units which are continuously scanned by antennas, and clutter maps are updated; the clutter maps are alternately updated; a low-speed target clutter map is calculated; clutter map area extension is carried out; a low-speed clutter map in an option register is extended; radar low-speed target detection is carried out; threshold processing is carried out on input radar data and the low-speed clutter map extension result, and a target detection result is output in a comparison mode.

Method for extracting L-tyrosine from fermentation liquor

The invention provides a method for extracting L-tyrosine from fermentation liquor. The method comprises the following steps: performing low-speed centrifugal separation to obtain an L-tyrosine crystal containing part of thalli and a bacterial suspension containing a saturated L-tyrosine solution; performing high-speed centrifugal separation on the bacterial suspension to obtain thalli and supernate, performing mixed resuspension and low-speed centrifugation on the supernate to obtain L-tyrosine crystals containing a small amount of thalli, and performing low-speed centrifugal separation on the crystals to obtain L-tyrosine crystals containing a very small amount of thalli; slowly dissolving all L-tyrosine crystals in the obtained L-tyrosine crystal suspension by using an acid solution to obtain an L-tyrosine solution containing a small amount of thalli, carrying out high-speed centrifugation to separate supernatant and thalli, recovering the thalli, decolorizing the supernatant by using ...

Method for detecting infrared puniness target

The invention discloses a detecting method of infrared weak and small objects. The method is characterized in that the method comprises the following steps: (1) an extended LBP operator matrix block with the size of 9*9 pixels is established and the 3*3 matrix block in the middle of the LBP operator matrix block is taken as a target area and 3*3 matrix blocks around the target area are taken as background areas in eight directions; (2) local signal-to-noise ratio is adopted as thresholds for carrying out coding on the background areas in the eight directions, thus obtaining binary system characteristic value of the background areas in the eight directions; (3) when the characteristic value is in accordance with judging standards, the 3*3 matrix block in the middle is taken as the target. The method has the advantages of improving the size and coding standards of traditional local dyadic mode operator, thus leading the method to be applicable to describing gray scale distribution of the ...

Amino acid yield related protein cynT as well as biological material and application thereof

The invention discloses amino acid yield related protein cynT as well as a biological material and application thereof. The technical problem to be solved is how to improve the microbial amino acid yield. The invention specifically discloses A1) protein, wherein the protein is cynT protein or cynT mutant protein obtained by replacing the 74th phenylalanine residue of the amino acid sequence of the cynT protein with other amino acid residues; the cynT protein is a protein of which the amino acid sequence is a sequence 2; a2) a protein which is obtained by substitution and/or deletion and/or addition of amino acid residues on the protein of A1), has 80% or more of identity with the protein of A1) and can increase the amino acid yield of microorganisms; and A3) a fusion protein which is obtained by connecting a tag to the N end and/or C end of A1) or A2) and has the same function. Substances that increase the expression of the cynT protein gene can be used to increase the amino acid yield ...

Production method of bio-based flame-retardant polyamide

The invention provides a production method of bio-based flame-retardant polyamide. The method comprises the following steps: extracting pentamethylene diamine with the purity of more than 99.9% and refined adipic acid with the purity of more than 99.9%, decoloring by using a stirring tank with a heat exchange system through a low-temperature salt forming technology and activated carbon under the protection of 99.999% high-purity nitrogen, reducing impurities in a nylon 56 salt solution, and carrying out continuous polymerization to obtain nylon 56 slices, short fibers or long fibers with the limit oxygen index of more than 32%, the polyamide material is initiated to reach a flame-retardant level under the condition that a flame retardant is not added.

Amino acid yield related protein coding gene rplP as well as biological material and application thereof

The invention discloses an amino acid yield related protein rplP as well as a biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the problem of regulating and controlling the yield of microbial amino acids, wherein the amino acids specifically can be L-threonine, L-tryptophan, L-arginine and L-valine. The protein is any one of the following proteins: A1) a protein of which the amino acid sequence is that isoleucine at the 73rd site of a sequence 2 is mutated into a sequence shown as leucine, phenylalanine, valine, aspartic acid, alanine or glycine; a2) a protein which is obtained by substitution and/or deletion and/or addition of amino acid residues on the amino acid sequence of A1), has 80% or more of identity with the protein shown in A1) and has the function of regulating and controlling the yield of microbial amino acid; and A3) a fusion protein which is obtained by connecting a tag to the N end and/or C end of A1) ...

Amino acid yield related protein cynT mutant as well as biological material and application thereof

The invention discloses an amino acid yield related protein cynTV13LQ46R as well as a biological material and application of the amino acid yield related protein cynTV13LQ46R. The technical problem to be solved is how to improve the microbial amino acid yield. The invention specifically discloses a protein which is any one of the following proteins: A1) a protein which is obtained by replacing the 13th valine residue of the amino acid sequence of the cynT protein with other amino acid residues, and A2) a protein which is obtained by replacing the 13th valine residue of the amino acid sequence of the cynT protein with other amino acid residues; a2) a protein which is obtained by substitution and/or deletion and/or addition of amino acid residues on the protein of A1), has 80% or more of identity with the protein of A1) and has the function of increasing the amino acid yield of microorganisms; and A2) a cynT mutant protein obtained by substitution and/or deletion and/or addition of amino ...

Method for improving yield and conversion rate of L-tyrosine

The invention provides a method for improving the yield and conversion rate of L-tyrosine, which adopts a two-stage continuous fermentation mode, performs graded fermentation culture in the middle and later periods of an acid-producing peak period through multi-equipment linkage, breaks the general rule of batch fermentation thallus growth, and supplements a fresh culture medium at the same time, so that thalli quickly enter the acid-producing peak period, and the yield and conversion rate of L-tyrosine are improved. The problem of acid production delay in the early stage of partial growth coupling type fermentation is well solved, the vitality of thalli is remarkably improved, product synthesis is promoted, the yield of L-tyrosine and the conversion rate of saccharic acid are increased, the fermentation period is effectively shortened, and the production intensity is improved. Meanwhile, a series of operations such as cleaning, charging and sterilizing of the secondary fermentation tank ...

Method for increasing yield of L-valine and recombinant bacteria used by same

The invention discloses a method for increasing the yield of L-valine and recombinant bacteria used by the method. The recombinant bacterium is an engineering bacterium which is obtained by knocking out a thiE gene and a mdh gene in escherichia coli (such as Escherichia coli YP045 CGMCC (China General Microbiological Culture Collection Center) No.22721 or escherichia coli W3110) capable of producing valine, or knocking out the thiE gene and the mdh gene at the same time. The yield of L-valine can be increased by fermenting the recombinant bacteria. The method has an important application value.

Method for improving L-tyrosine fermentation yield and sugar-acid conversion rate

The invention provides a method for improving the fermentation yield of L-tyrosine and the sugar-acid conversion rate. The L-tyrosine is produced by adopting escherichia coli engineering bacteria TYR-05 through a microbial fermentation method. Methionine, lysine, glutamic acid, Cu < 2 + >, Zn < 2 + >, Co < 2 + > and Mn < 2 + > are subjected to chelation reaction to prepare the oligopeptide microelement mixed chelating agent, and important auxiliary factors Cu < 2 + >, Zn < 2 + > and other microelements of enzymes in biochemical reaction are generated into amino acid chelates with more stable chemical energy, so that the loss of the microelements in sterilization and fermentation engineering is effectively prevented; in the fermentation process, a certain amount of oligopeptide microelement mixed chelating agent is fed, and the feeding speed is adjusted along with the change of the dissolved oxygen value so as to maintain the sub-appropriate nutrition supply, so that the vitality of thalli ...

METHOD FOR FERMENTATION-PRODUCTION OF PENTANEDIAMINE COMPRISING CARBON DIOXIDE STRIPPING TECHNIQUE

A method for fermentation-production of a pentanediamine, comprising: culturing a cell expressing a lysine decarboxylase to obtain a whole cell fermentation broth comprising a pentanediamine; and extracting the pentanediamine from the whole cell fermentation broth, and striping the whole cell fermentation broth of carbon dioxide contained therein before adding a strong base. The method greatly increases a production volume of the pentanediamine. 1. A method for preparing 1 ,5-pentanediamine by fermentation , comprising:(1) culturing a cell expressing a lysine decarboxylase to obtain a fermentation broth comprising 1, 5-pentanediamine;(21) separating the liquid from the fermentation broth;(22) removing carbon dioxide from the liquid obtained in the step (21), and then adding a base for treatment;(23) extracting a composition containing 1,5-pentanediamine from the treatment liquid obtained in the step (22).2. The method according to claim 1 , wherein in step (1) claim 1 , the cell is a cell overexpressing lysine decarboxylase.3. The method according to claim 1 , wherein in step (1) claim 1 , the cell is a cell with enhanced lysine decarboxylase gene expression.4. The method according to claim 1 , wherein in step (1) claim 1 , the cell is a cell whose expression is enhanced by replacing the promoter of the lysine decarboxylase gene with a strong promoter.5. The method according to claim 1 , wherein in step (1) claim 1 , the cell is a cell whose expression is enhanced by replacing the promoter of the lysine decarboxylase gene with T7 promoter.6. The method according to claim 1 , wherein in step (1) claim 1 , the cell is a bacterial cell.7E. coli. The method according to claim 1 , wherein in step (1) claim 1 , the cell is an cell.8E. coli. The method according to claim 1 , wherein in step (1) claim 1 , the cell is BL21 (DE3).9. The method according to claim 1 , wherein in step (1) claim 1 , no acidic pH regulator is added to the culture claim 1 , and the cells themselves ...

Production method for extracting tryptophan from fermentation liquor by one-step refining

The invention discloses a production method for extracting tryptophan from fermentation liquor by one-step refining, which comprises the pretreatment of the fermentation liquor. In the method, mycoprotein is filtered off by using a ceramic microfiltration membrane, is decolorized by using an ultrafiltration membrane and is desalted and concentrated by using a nanofiltration membrane; the nanofiltration membrane concentrated liquor is directly decolorized by using activated carbon, the decolorized liquor is subjected to isoelectric point crystallization at a low temperature, and a qualified competitive product is obtained by rinsing with ice water during centrifugal separation; the crystallization mother liquor returns to the nanofiltration membrane for circulation; protein feed with high additional value is prepared by mixing, drying and crushing the ultrafiltration strong liquor and mycoprotein; and nanofiltration strong brine can be used as a culture medium for later use or sold serving ...

Ejector pin with adjustable

The utility model relates to an ejector pin with adjustable belongs to the passenger car production field. The left -hand screw is female to be welded with the sheathed tube left end, right -handed nut and the welding of sheathed tube left end, and left pipe and the welding of left gusset, left pipe and the welding of right gusset, left gusset welds with the right spiral pole with left -hand screw pole welding, right gusset, left -hand screw pole and levogyration nut and threaded connection, right spiral pole and dextrorotation nut and threaded connection have the through -hole on the sleeve pipe, and the swing arm is placed in the through -hole. The utility model has the advantages of: novel structure is simple, convenient to use, and maneuverability is strong, can adjust according to the size of work piece, to carrying out accurate positioning adjustment after the welding of work piece to reach the size of requirement, can be used to the position that the various welding position of having ...

Escherichia coli, construction method thereof and application of escherichia coli in production of L-valine

The invention discloses Escherichia coli, a construction method thereof and application of the Escherichia coli in production of L-valine. According to the invention, after a yjiV gene in escherichia coli is knocked out and ilvE and ilvD are expressed, or a trpR gene is knocked out and ilvH mutant protein is expressed, lacI and lacZ genes are knocked out and DNA polymerase is expressed, and a ycgH gene is knocked out and ilvC is expressed, the obtained recombinant bacterium can improve the yield of L-valine, can be used for producing L-valine, and has a very good application prospect.

Modified Escherichia coli and application thereof in fermentation production of L-valine

The invention discloses modified Escherichia coli and application of the modified Escherichia coli in fermentation production of L-valine. The method comprises the following steps: in escherichia coli for producing valine, knocking out a pflB gene, knocking out the pflB gene and an adhE gene, knocking out the pflB gene and the adhE gene and knocking out an ilvE (E) gene and simultaneously inserting an ilvE (B) gene from bacillus subtilis, knocking out the pflB gene, the adhE gene and a thiE gene and knocking out the ilvE (E) gene and simultaneously inserting the ilvE (B) gene from the bacillus subtilis, or knocking out the pflB gene and the adhE gene; the engineering bacteria obtained by knockout of the hiE gene and the mdh gene and insertion of the ilvE (E) gene from bacillus subtilis can improve the yield of L-valine, and the engineering bacteria have important application value.

Agricultural deep water-saving device for groundwater irrigation of Mauwu sand

The invention discloses an agricultural deep water-saving device for groundwater irrigation of a Maowu sandy land. The agricultural deep water-saving device comprises a plurality of main pipelines pre-buried in a land parcel; the fixed plate is fixedly connected to a land parcel; the water distribution pipes are fixedly connected between the main pipeline and the fixing plate, the water distribution pipes are communicated with the main pipeline, the water distribution pipes are arranged on one side of the plant column, and the water distribution pipes are parallel to the plant column; the water distribution pipes are connected with the main pipeline through sealing pieces; a plurality of water outlets are formed in the main pipeline, and the water inlet of one water distribution pipe is connected with one water outlet of the main pipeline through a sealing piece; when the device is used, a water source is injected into the main pipeline through the first water suction pump, the water source ...

Escherichia coli for producing L-valine and construction method thereof

The invention discloses escherichia coli for producing L-valine and a construction method of the escherichia coli. According to the invention, after lacI and lacZ genes in escherichia coli are knocked out and DNA polymerase is expressed, or a ycgH gene is knocked out and ilvC is expressed in a combined manner, the obtained recombinant bacterium can improve the yield of L-valine, can be used for producing L-valine, and has a very good application prospect.

Method for reducing foam in L-tyrosine fermentation process

The invention provides a method for reducing foam in an L-tyrosine fermentation process, which comprises the following steps of: adding a proper amount of L-tyrosine seed crystal at the initial stage of L-tyrosine crystallization and when a large amount of foam is generated by L-tyrosine fermentation to enlarge crystals and accelerate the crystallization speed of L-tyrosine; meanwhile, crystals are enlarged and the crystal form is optimized through an ultrasonic-assisted crystallization method, so that the problem of excessive foam in the fermentation process caused by L-tyrosine crystallization is reduced; the method solves the problem of serious foaming of fermentation liquor caused by fine crystals in the fermentation process of L-tyrosine in the prior art; meanwhile, the consumption of a defoaming agent (the effective component is a surfactant) is reduced, the negative influence caused by excessive use of the surfactant in the thallus growth process is avoided, and the method has important ...

Threonine dehydrogenase gene mutant and application thereof

The invention discloses a threonine dehydrogenase gene mutant and application thereof, belongs to the technical field of biology, and aims to solve the technical problem of how to improve the yield of microorganisms L-valine or L-isoleucine. In particular, the present invention discloses an engineered threonine dehydrogenase comprising a mutation in the amino acid at position 131, 171 or 218 relative to a reference sequence (SEQ ID No.2). Recombinant bacteria for overexpressing ilvAV131A, ilvAR171H and/or ilvAM218I genes and point mutation recombinant bacteria are constructed, and results show that overexpression of mutant genes can improve the yield of L-valine and/or L-isoleucine of recipient bacteria, and has the effect of synergistically promoting production of L-valine and/or L-isoleucine, so that the recombinant bacteria can be used for preparing the recombinant bacteria for treating L-valine and/or L-isoleucine, and the recombinant bacteria can be used for preparing the recombinant ...

Recombinant escherichia coli for fermentation production of L-valine

The invention discloses recombinant escherichia coli for fermentation production of L-valine. The recombinant Escherichia coli is obtained by knocking out an adhE gene in Escherichia coli for producing valine, knocking out the adhE gene and knocking out an ilvE (E) gene and inserting an ilvE (B) gene from bacillus subtilis at the same time, knocking out the adhE gene and a thiE gene and knocking out the ilvE (E) gene and inserting the ilvE (B) gene from bacillus subtilis at the same time, or knocking out the adhE gene. The engineering bacterium is obtained by knockout of a thiE gene and an mdh gene and insertion of an ilvE (B) gene from bacillus subtilis while the ilvE (E) gene is knocked out, the yield of L-valine can be increased by fermenting recombinant escherichia coli, and the engineering bacterium has important application value.

A hang wherein heart nozzle for lime shaft kiln

The utility model provides a hang wherein heart nozzle for lime shaft kiln, belongs to the lime shaft kiln and calcines technical field, and the lime shaft kiln structure is simplified to the purpose of design, reduces its manufacturing cost, and its technical scheme is, and this center nozzle includes a nozzle body and a supporting beam, prop up supporting beam and fix the kiln body top at the lime shaft kiln, the nozzle body is located supporting beam's below, and its upper end is fixed on a supporting beam, and the internal calcining zone of the gas exports of nozzle body lower extreme and kiln is corresponding. The utility model discloses a suspension type structure, the gas exports of nozzle has avoided traditional low nozzle to influence lime refrigerated problem in the position of calcining zone, this nozzle need not to install additional after hood and the slope and consequently need not install the fixed beam additional at automatic recovery vertical position under the effect of ...

BBD2914255 gene mutant and application thereof in preparation of L-glutamic acid

The invention discloses a BBD2914255 gene mutant and an application of the BBD2914255 gene mutant in preparation of L-glutamic acid. The BBD2914255 gene mutant disclosed by the invention is DNA (Deoxyribose Nucleic Acid) molecules as shown in SEQ ID No.3, 5 and 7 in a sequence table and is used for coding proteins as shown in SEQ ID No.4, 6 and 8, and the wild type BBD2914255 gene is a DNA molecule as shown in SEQ ID No.1 and is used for coding a protein as shown in SEQ ID No.2. Experiments prove that the BBD2914255 gene and the mutant thereof can improve the yield of the L-glutamic acid, can be used for producing the L-glutamic acid, and have a very good application prospect.

Method for improving yield and conversion rate of L-tyrosine

The invention provides a method for improving yield and conversion rate of L-tyrosine, which comprises the following steps: adding a methyl donor and one part of amino acid chelated trace elements into a fermentation medium, feeding and fermenting the other part of the amino acid chelated trace elements along with glucose, the methyl donor is betaine and choline chloride, the amino acid chelated trace elements are methionine chelated iron and methionine chelated manganese, and the glucose is glucose. By adding the methyl donors and the amino acid chelated trace elements, on one hand, the supply amount of methyl groups, amino acids and trace elements in the fermentation process is increased, and on the other hand, the methyl donors, the amino acids and the trace elements can participate in metabolic pathways related to thalli, so that the metabolic capability and the production performance of the thalli are improved; on the other hand, betaine and choline chloride can maintain and regulate ...

Directional transformation method of L-tyrosine efficient production strain and production strain

The invention provides a directional transformation method of an L-tyrosine efficient production strain and the production strain, the method comprises the following steps: firstly, carrying out conventional production fermentation of tyrosine, and removing thalli, tyrosine and other solids in a fermentation system through a coupled continuous butterfly centrifuge respectively when the fermentation production is carried out for 22, 28 and 34 hours; and performing adaptive evolution on the existing L-tyrosine production strain in sequence (22h-28h-34h) by using the fermentation supernatant from which the solid matter is removed, performing directional transformation culture for 36 hours, diluting, coating and separating to finally obtain the strain with high adaptability to the growth environment in the later fermentation period of the strain, the obtained strain can better adapt to the thallus growth environment in the later fermentation period, the thallus activity and acid production ...

Method for Producing L-Lysine by Modifying Aconitase Gene and/or Regulatory Elements thereof

A method for producing L-lysine by fermentation comprises the steps of modifying an aconitase gene and/or regulatory element thereof in a chromosome of a bacterium so that the activity and/or the expression amount of the aconitase of the bacterium are reduced but not eliminated; and producing L-lysine by the fermentation of the modified bacterium. In addition, also provided are methods and uses derived from the method as well as bacteria used in these methods and uses. 1. A method of producing L-lysine by fermentation or of increasing a fermentation yield of L-lysine , which comprises the steps of:(1) modifying an aconitase gene and/or regulatory element thereof in a chromosome of a bacterium so that the activity and/or the expression amount of the aconitase of the bacterium are reduced but not eliminated; and(2) producing L-lysine by fermentation with the bacterium obtained by the modification of step (1).2. A use of a bacterium obtained by a modification for producing L-lysine by fermentation or for increasing a fermentation yield of L-lysine , wherein the modification is to modify an aconitase gene and/or regulatory element thereof in a chromosome of a bacterium , and the activity and/or the expression amount of the aconitase of the bacterium obtained by the modification are reduced but not eliminated.3. The method according to claim 1 , which comprises the step of modifying an aconitase gene and/or regulatory element thereof in a chromosome of a bacterium so that the activity and/or the expression amount of the aconitase of the bacterium obtained by the modification are reduced but not eliminated.43. The method according to claim 1 , wherein the step of modifying an aconitase gene in a chromosome of a bacterium is an addition claim 1 , deletion or substitution of one or more nucleotides in the nucleotide sequence of the aconitase gene.5. The method according to claim 4 , wherein the substitution includes a substitution for the initiation codon of the aconitase ...

Application of transport carrier gene which improves l-tryptophan production efficiency in escherichia coli

A transport protein coding gene, and a method for efficient production of L-tryptophan by a strain containing the gene. Specifically, by heterologous expression of ywkB gene from Bacillus subtilis on the genome of Escherichia coli, L-tryptophan production efficiency of the strain can be improved. Performing shake flask fermentation with the strain can accumulate 15.2 g/L of L-tryptophan within 24 h, which is 35% higher than a control strain.

Method for producing l-lysine by modifying aconitase gene and/or regulatory elements thereof

Provided is an L-lysine generation method by fermentation, comprising modifying the aconitase gene and/or regulatory element thereof on a bacteria chromosome, such that the enzymatic activity and/or expression quantity of the aconitase are reduced but do not disappear; and generating L-lysine by fermenting the modified bacteria. Also disclosed are methods and uses derived from the present method, and the bacteria that can be used in these methods and uses.

Application of transport carrier gene which improves l-tryptophan production efficiency in escherichia coli

Method for fermentation-production of pentanediamine comprising carbon dioxide stripping technique

Escherichia coli-based recombinant strain, construction method therefor and use thereof

The present disclosure discloses an Escherichia coli-based kdtA -gene-modified recombinant strain, a construction method therefor and use thereof. A mutant gene obtained by subjecting a wild-type kdtA gene (ORF sequence is shown in a sequence 73556-74833 in GenBank accession No. CP032667.1), a wild-type spoT gene (ORF sequence is shown in a sequence 3815907-3818015 in GenBank accession No. AP009048.1) and a wild-type yebN gene (ORF sequence is shown in a sequence 1907402-1907968 in GenBank accession No. AP009048.1) of an E. coli K12 strain and a derivative strain thereof (such as MG1655 and W3110) to site-directed mutagenesis, and a recombinant strain obtained therefrom can be used for the production of L-threonine, and compared with an unmutated wild-type strain, the obtained strain can produce L-threonine with a higher concentration and has good strain stability, and also has lower production cost as an L-threonine production strain.

Strain having enhanced l-glutamic acid productivity, construction method therefor and application thereof

Disclosed are strain having enhanced L-glutamic acid production capacity, and method for constructing the same and use thereof. A nucleotide sequence is provided by introducing a point mutation to a wild-type BBD29-00405 gene in Corynebacterium glutamicum so that the base at position 597 of SEQ ID NO: 1 is mutated from guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing the polynucleotide sequence into L-glutamic acid-producing Corynebacterium glutamicum, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with an unmodified strain, the resulting strain facilitates production of L-glutamic acid at a higher concentration.

Escherichia coli-based recombinant strain and construction method therefor and application thereof

The present disclosure discloses an Escherichia coli-based genetically-modified recombinant strain, a construction method therefor and use thereof. A mutant gene obtained by subjecting a wild-type deoB gene (ORF sequence is shown in a sequence 3902352-3903575 in GenBank accession No. CP032667.1) and a wild-type rhtA gene promoter sequence PrhtA (shown in a sequence 850520-850871 in GenBank accession No. AP009048.1) of an E. coli K12 strain and a derivative strain thereof (such as MG1655 and W3110) to site-directed mutagenesis, and a recombinant strain obtained therefrom can be used for the production of L-threonine, and compared with an unmutated wild-type strain, the obtained strain can produce L-threonine with a higher concentration and has good strain stability, and also has lower production cost as an L-threonine production strain.

Recombinant strain for producing l-amino acid, construction method therefor, and application thereof

A bacterium for producing L-amino acid has improved expression of a polynucleotide encoding a protein represented by SEQ ID NO:3 and improved expression of a polynucleotide encoding a protein represented by SEQ ID NO:31, and/or has mutations in bases at positions −45 bp and −47 bp of a promotor region represented by SEQ ID NO:57. A polynucleotide, encodes proteins and can be included in a recombinant vector, which can be included in a recombinant strain. These are useful in a method for producing L-amino acid. The polynucleotide encodes a protein which is represented by SEQ ID NO:3 and has arginine at position 334 substituted by a terminator or encodes a protein which is represented by SEQ ID NO:31 and has tyrosine at position 592 substituted by phenylalanine, or is formed by mutations in bases at positions −45 bp and −47 bp of a promotor region represented by SEQ ID NO:57.

Recombinant strain of modifying gene bbd29_14900, and construction method and use thereof

Provided are a recombinant strain with modified gene BBD29_14900, and a method for constructing the same and use thereof, with the production of L-glutamic acid as a specific application. Further provided is a method for introducing a point mutation into the BBD29_14900 gene coding sequence in Corynebacterium or improving the expression thereof. The method can cause a bacterial strain with the mutation to increase the fermentation yield of glutamic acid. The point mutation involves a mutation of the base at position 1114 in the sequence of the BBD29_14900 gene from guanine (G) to adenine (A), and thus a substitution of aspartic acid at position 372 in the coded corresponding amino acid sequence with asparagine.

Recombinant strain with modified gene bbd29_14900, and method for constructing the same and use thereof

Provided are a recombinant strain with modified gene BBD29_14900, and a method for constructing the same and use thereof, with the production of L-glutamic acid as a specific application. Further provided is a method for introducing a point mutation into the BBD29_14900 gene coding sequence in Corynebacterium or improving the expression thereof. The method can cause a bacterial strain with the mutation to increase the fermentation yield of glutamic acid. The point mutation involves a mutation of the base at position 1114 in the sequence of the BBD29_14900 gene from guanine (G) to adenine (A), and thus a substitution of aspartic acid at position 372 in the coded corresponding amino acid sequence with asparagine.

Recombinant strain producing l-lysine and construction methods therefor and use thereof

Provided are a method for introducing point mutations to the coding sequence of NCg12176 gene or improving the expression thereof in Corynebacterium glutamicum , and a method for performing point mutations on the promoter region sequence of dapB gene in Corynebacterium glutamicum . The fermentation yield of L-lysine produced by a strain with the mutations can be increased by means of the methods.

Cepa recombinante que produce l-lisina y metodos de construccion para la misma y uso de la misma.

Se proporcionan un método para introducir mutaciones puntuales en la secuencia codificante del gen NCg12176 o mejorar la expresión del mismo en Corynebacterium glutamicum, y un método para realizar mutaciones puntuales en la secuencia de la región promotora del gen dapB en Corynebacterium glutamicum. Por medio de los métodos el rendimiento de fermentación de L-lisina producida por una cepa con las mutaciones puede ser incrementado.

Escherichia coli-based recombinant strain and construction method therefor and application thereof

The present disclosure discloses an Escherichia coli-based genetically-modified recombinant strain, a construction method therefor and use thereof. A mutant gene obtained by subjecting a wild-type deoB gene (ORF sequence is shown in a sequence 3902352-3903575 in GenBank accession No. CP032667.1) and a wild-type rhtA gene promoter sequence P rhtA (shown in a sequence 850520-850871 in GenBank accession No. AP009048.1) of an E . coli K12 strain and a derivative strain thereof (such as MG1655 and W3110) to site-directed mutagenesis, and a recombinant strain obtained therefrom can be used for the production of L-threonine, and compared with an unmutated wild-type strain, the obtained strain can produce L-threonine with a higher concentration and has good strain stability, and also has lower production cost as an L-threonine production strain.

自动开门人脸识别一体机

本实用新型公开了一种自动开门人脸识别一体机，包括一门体，设于门体一侧的一人脸识别一体机箱，门体的下端固定于一下旋转固定板上，一开门机安装在人脸识别一体机箱上，一输出轴固定连接下旋转固定板，通过转动输出轴带动门体，门体的上端固定于一上旋转固定板上，一旋转轴固定连接人脸识别一体机箱，通过转动旋转轴带动上旋转固定板和门体转动，一电机设于人脸识别一体机箱内，电机对输出轴提供动力，带动输出轴转动，一人脸识别控制系统设于人脸识别一体机箱内，人脸识别控制系统对电机进行控制，当人脸识别控制系统通过人脸识别时，电机工作带动输出轴转动，输出轴带动门体打开，实现人脸识别与自动开门，人脸识别无感通行功能。

Escherichia coli-based recombinant strain, construction method therefor and use thereof

The present disclosure discloses an Escherichia coli-based kdtA-gene-modified recombinant strain, a construction method therefor and use thereof. A mutant gene obtained by subjecting a wild-type kdtA gene (ORF sequence is shown in a sequence 73556-74833 in GenBank accession No. CP032667.1), a wild-type spoT gene (ORF sequence is shown in a sequence 3815907-3818015 in GenBank accession No. AP009048.1) and a wild-type yebN gene (ORF sequence is shown in a sequence 1907402-1907968 in GenBank accession No. AP009048.1) of an E. coli K12 strain and a derivative strain thereof (such as MG1655 and W3110) to site-directed mutagenesis, and a recombinant strain obtained therefrom can be used for the production of L-threonine, and compared with an unmutated wild-type strain, the obtained strain can produce L-threonine with a higher concentration and has good strain stability, and also has lower production cost as an L-threonine production strain.

Recombinant strain for producing l-glutamic acid by means of modifying gene bbd29_04920, and construction method and use thereof

Provided is a method for introducing a point mutation to a BBD29_04920 gene coding sequence in Corynebacterium or improving the expression thereof. The point mutation causes a mutation to the base at position 1560 in the BBD29_04920 gene sequence from cytosine (C) to adenine (A) such that asparagine at position 520 of a coded corresponding amino acid sequence is substituted by lysine. The method can increase fermentation yield of glutamic acid in a strain with the mutation. Also provided are the bacterium generating L-glutamic acid, a nucleic acid and protein comprising the mutation, a recombinant vector and recombinant strain comprising the nucleic acid, and use of these biomaterials in the regulation of the production of L-glutamic acid of a microorganism.

Recombinant strain for producing l-glutamic acid by means of modifying gene bbd29_11265, and construction method and use thereof

The present invention discloses recombinant strain with modified gene BBD29_11265 for producing L-glutamic acid, and method for constructing the same and use thereof. The recombinant strain is a bacterium that generates L-glutamic acid, and has an improved expression of a polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof; the improved expression can be having a point mutation in, and an enhanced expression of the polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof. A genetically engineered bacterium in which the base at position 70 in the BBD29_112665 gene sequence is mutated to adenine from guanine, causing alanine at position 24 in the coded corresponding amino acid sequence to be substituted with threonine, and an engineered bacterium overexpressing the BBD29_112665 gene or BBD29_11265 G70A gene are constructed in the present invention, facilitating an increase in the production and conversion rate of L-glutamic acid.

Strain having enhanced l-glutamic acid productivity, construction method therefor and application thereof

Disclosed are strain having enhanced L-glutamic acid production capacity, and method for constructing the same and use thereof. A nucleotide sequence is provided by introducing a point mutation to a wild-type BBD29-00405 gene in Corynebacterium glutamicum so that the base at position 597 of SEQ ID NO: 1 is mutated from guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing the polynucleotide sequence into L-glutamic acid-producing Corynebacterium glutamicum, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with an unmodified strain, the resulting strain facilitates production of L-glutamic acid at a higher concentration.

Strain having enhanced l-glutamic acid productivity, construction method therefor and application thereof

Disclosed are strain having enhanced L-glutamic acid production capacity, and method for constructing the same and use thereof. A nucleotide sequence is provided by introducing a point mutation to a wild-type BBD29-00405 gene in Corynebacterium glutamicum so that the base at position 597 of SEQ ID NO: 1 is mutated from guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing the polynucleotide sequence into L-glutamic acid-producing Corynebacterium glutamicum, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with an unmodified strain, the resulting strain facilitates production of L-glutamic acid at a higher concentration.

Recombinant strain with modified gene bbd29_11265 for producing l-glutamic acid, and method for constructing the same and use thereof

A recombinant strain with modified gene BBD29_11265 and a method for constructing the same are provided. The recombinant strain is a bacterium that generates L-glutamic acid, and has an improved expression of a polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof; the improved expression can be having a point mutation in, and an enhanced expression of the polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof. A genetically engineered bacterium in which the base at position 70 in the BBD29_112665 gene sequence is mutated to adenine from guanine, causing alanine at position 24 in the coded corresponding amino acid sequence to be substituted with threonine, and an engineered bacterium overexpressing the BBD29_112665 gene or BBD29_11265 G70A gene are constructed in the present invention, facilitating an increase in the production and conversion rate of L-glutamic acid.

Recombinant strain for producing l-amino acid and construction method and use thereof

Taking Corynebacterium glutamicum YP97158 as the starting bacterium, introducing site-directed mutation and/or expression enhancement in the coding region of its NCgl1089 gene, the coding region of NCgl0761 gene, and/or the coding region of ptsS gene, the obtained mutant gene and the recombination comprising said gene has high-efficiency L-amino acids production capacity, which greatly increases the output of L-amino acids, and the strain has good stability, which reduces the production cost as an L-amino acids production strain.

Recombinant strain for producing l-glutamic acid by modifying gene bbd29_09525, and construction method therefor and application thereof

Recombinant strain with modified gene BBD29_09525 for producing L-glutamic acid, and method for constructing the same and use thereof are provided. The recombinant strain is a bacterium that generates L-glutamic acid, and has an improved expression of a polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof; the improved expression can be having a point mutation in, and an enhanced expression of the polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof. The genetically engineered bacterium is an engineered bacterium that has a mutation of the base at position 337 in a BBD29_09525 gene sequence from cytosine to thymine, causing proline at position 113 in the coded corresponding amino acid sequence to be substituted with serine, and that overexpresses the BBD29_09525 gene or BBD29_09525^C337T gene. The genetically engineered bacterium enables increased production and conversion rate of L-glutamic acid.

Recombinant strain for producing l-amino acid, and construction method therefor and use thereof

Taking Corynebacterium glutamicum YP97158 as the starting bacterium, introducing site-directed mutation and/or expression enhancement in the coding region of its NCgl1089 gene, the coding region of NCgl0761 gene, and/or the coding region of ptsS gene, the obtained mutant gene and the recombination comprising said gene has high-efficiency L-amino acids production capacity, which greatly increases the output of L-amino acids, and the strain has good stability, which reduces the production cost as an L-amino acids production strain.

Recombinant strain with modified gene bbd29_04920 for producing l-glutamic acid, and method for constructing the same and use thereof

Provided is a method for introducing a point mutation to a BBD29_04920 gene coding sequence in Corynebacterium or improving the expression thereof. The point mutation causes a mutation to the base at position 1560 in the BBD29_04920 gene sequence from cytosine (C) to adenine (A) such that asparagine at position 520 of a coded corresponding amino acid sequence is substituted by lysine. The method can increase fermentation yield of glutamic acid in a strain with the mutation. Also provided are the bacterium generating L-glutamic acid, a nucleic acid and protein comprising the mutation, a recombinant vector and recombinant strain comprising the nucleic acid, and use of these biomaterials in the regulation of the production of L-glutamic acid of a microorganism.

Recombinant strain for producing l-amino acid, construction method therefor, and application thereof

Recombinant strain producing l-lysine and construction methods therefor and use thereof