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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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26-03-2014 дата публикации

Expression method of Beclin1 and MAP1-LC3 (microtubule-associated protein 1-light chain 3) in steroid-induced avascular necrosis of femoral head of rabbit

Номер: CN103675251A
Автор: LIU WANLIN, BAI RUI, ZHAO ZHENQUN, WANG WENXUAN, WANG YONG
Принадлежит:

The invention discloses an expression method of Beclin1 and MAP1-LC3 (microtubule-associated protein 1-light chain 3) in steroid-induced avascular necrosis of femoral head of a rabbit, detection of a bone cell apoptosis index of a femoral head tissue of a rabbit SANFH (steroid-induced avascular necrosis of femoral head) model and the MAP1-LC3 expression shows that autophagy-related gene Beclin1 can regulate autophagy activity and also can selectively combine with family members of Bcl-2 (B-cell lymphoma-2) to regulate the cell apoptosis activity. By detection of the expression of the Beclin1 in the SANFH model, the expression method provides a new molecular target for treatment of SANFH. The expression method is simple and convenient in operation, better solves the problem that no study on cell apoptosis and autophagy expression and mutual relation thereof in the SANFH model is found at home and abroad, and fills the blank of the study on cell apoptosis and autophagy expression and the ...

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Номер записи: 1
26-03-2014 дата публикации

Experimental method of effects of DNA oxidative damage and bone cell apoptosis on avascular necrosis

Номер: CN103675250A
Автор: LIU WANLIN, ZHAO ZHENQUN, BAI RUI, WANG WENXUAN, WANG YONG
Принадлежит:

The invention discloses an experimental method of effects of DNA oxidative damage and bone cell apoptosis on avascular necrosis, the method comprises the following steps: experimental grouping and establishment of an animal model, preparation of specimens, histomorphology observation under a light microscope, observation by a transmission electron microscope, bone cell apoptosis situation detection by TUNEL (terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling) method, bone marrow hematopoietic cell DNA oxidative damage detection by monoclonal antibody N45.1 immunohistochemistry method, and statistical analysis. According to the method, experiment animals are respectively killed in the second week and the fourth week after the last time of administration, and five animals are killed for each time in each group; bilateral femoral heads are taken for conventional fixed decalcification for stand-by testing, the number of vacant bone lacunas is counted by HE (hematoxylin-eosin ...

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Номер записи: 2