Plant circRNA expression framework and application thereof

The invention discloses a plant circRNA expression framework and application thereof, and belongs to the technical field of molecular biology. The method comprises the following steps: putting a Vv-circSIZ1 upstream flanking intron sequence and a Vv-circSIZ1 downstream flanking intron sequence on two sides of low-expression circRNA (Ribonucleic Acid) to construct a plant circRNA expression framework; furthermore, a circRNA-OE vector plasmid constructed in an earlier stage is used as a vector skeleton, and a plant circRNA expression framework is inserted between BamHI and PstI of the vector skeleton through a homologous recombination method, so that the plant circRNA overexpression vector is constructed. By adopting the plant circRNA expression framework and the plant circRNA overexpression vector disclosed by the invention, the cyclization efficiency of low-expression circRNA can be greatly improved, the difficulty of constructing a vector by circRNA containing a long intron is reduced, ...

Application of U2AF65b in regulation and control of plant salt stress resistance

The invention discloses application of U2AF65b in regulation and control of plant salt stress resistance, and belongs to the technical field of biology. According to the invention, a splicing factor gene, namely U2AF65b, is cloned from grapes. Transgenic experiments prove that U2AF65b participates in salt resistance, so that cognition on generation of salt resistance of plants is expanded, a theoretical basis is provided for obtaining high-salt-resistance plants, and the application value is great.

Method for improving antioxidant capacity and quality of seeds

The invention relates to a method for improving antioxidant capacity and quality of seeds. In the method, the recombination of a seed-specific promoter At2S3 and three antioxidant genes is applied to improve the antioxidant capacity of the seeds. The upstream 1760 bp promoter sequence of the At2S3 gene respectively fuses GUS (beta-glucuronidase) reporter gene, manganese-superoxide dismutase (MSD1) gene, catalase (CAT1) gene and a gene of a key enzyme (homogentisate plant-transferase; HPT1) during vitamin E biosynthesis to transform arabidopsis and carry out strain hybridization. The results show that the GUS reporter gene is specifically and efficiently expressed in the seeds; and the monovalent MSD1 (M7), bivalent MSD1/CAT1 (M7/C9) and trivalent MSD1/CAT1/HPT1 (M7/C9/H3) transgenic arabidopsis strains have significantly higher germination rate of seeds when being subjected to 10 mu M paraquat oxidative stress treatment and significantly higher survival rate of seedlings when subjected ...

Method for extracting RNA in gymnosperm tissue

The invention discloses a method for extracting RNA from gymnosperm tissues. The method includes the following steps: 1) extracting buffer solution by RNA to treat plant materials; 2) protein removal: extracting by equal-volume chloroform/isoamylol for twice; 3) using LiCl to deposit RNA and SSTE to dissolve back RNA; 4) glycan removal: adding absolute ethyl alcohol and potassium acetate to the dissolved solution acquired in step 3) and depositing glycan in ice bath; 5) extracting chloroform/isoamylol again; 6) depositing RNA and DEPC H2O again to dissolve back RNA. In order to remove DNA, the following steps can be also conducted: a) using DNase without RNase pollution to remove DNA; b) using chloroform/isoamylol to extract and remove DNase; (c) repeating step 6). Wherein, RNA extract and buffer solution contains 100mmol/L Tris HCl(pH 8.0), 2g/100mlCTAB, 2g/100mlPVP, 2mol/LNaCl and 25mmol/L EDTA and is added with dithiothreitol with final concentration of 10mmol/L and 2 percent (volume ...

Picea wilsonii transcription factor PwHAP5 and coding gene and application thereof

The invention discloses a picea wilsonii transcription factor PwHAP5 and a coding gene and application thereof. The PwHAP5 is protein disclosed in 1) or 2): 1) the protein composed of an amino acid sequence disclosed in a sequence 2 in a sequence table; 2) the protein which is performed with substitution and/ or deletion and/ or adding of one of multiple amino acid residue by the amino acid sequence disclosed in the sequence 2 in the sequence table, is relevant to plant seed germination and is derived by 1). After the coding gene of PwHAP5 protein is guided into arabidopsis, the germination rate of the seed of transgenosis arabidopsis is obviously higher than that of wild arabidopsis in high-salt and wild environment.

Automatic measurement method for optical performance of head-up display

The invention discloses a head-up display optical performance automatic measurement method, which comprises the following steps of: firstly, constructing a head-up display optical performance automatic measurement device which comprises a control machine, a five-axis precision adjustment table, a head-up display, a collimator and a CCD (Charge Coupled Device) camera; the control machine is provided with test software and is electrically connected with the five-axis precision adjustment table and the CCD camera; the head-up display is placed between the five-axis precision adjustment table and the collimator; the five-axis precision adjustment table enables the picture center of the CCD camera to be automatically aligned with the optical axes of the head-up display and the parallel light tube under the automatic control of test software; meanwhile, the CCD camera is adjusted to the eye box position of the head-up display for testing; the test software superposes the virtual reticle image ...

Coaxial afocal total reflection type optical system assembling and adjusting method

The invention belongs to the field of precise optical machine installation and adjustment, and particularly relates to an installation and adjustment method for a coaxial afocal total reflection optical system, which realizes precise and rapid installation and adjustment of a coaxial double-paraboloid afocal total reflection optical system with an intermediate image plane. The invention designs an intermediate image plane reflex compensating mirror which can assist in collimation with an original optical path intermediate image plane reflex mirror, and provides a method for calibrating the reflex compensating mirror by an auto-collimation light pipe and a standard plane mirror, so as to finish the precise adjustment and fixation of the reflex compensating mirror. A laser interferometer and a standard ball are adopted to complete establishment of a primary mirror installation and adjustment reference, precise assembly of a middle image plane turning mirror is completed through the calibrated ...

Protein related to pollen germination and/or pollen tube growth, coding gene and use thereof

The invention discloses a protein related to pollen grain germination and/or pollen tube growth, the encoding genes and the application thereof. The protein is the following protein shown in (1) or (2): (1) the protein being composed of amino acid sequences shown in sequence two of a sequence table; (2) the protein comprises the amino acid in the sequence two of the sequence table substituted and/or lacked and/or added and is related to the stress tolerance of plant. The protein and the encoding gene thereof related to the pollen grain germination and/ or pollen tube growth can be used for the genetic transformation research of the plant, increases the pollen germination rate of plant and/or the growth speed of the pollen tube and the reproductive efficiency of the plant, shortens the breeding process, has important theoretical and practical actual meaning for adjusting and controlling the reproductive development and gene engineering breeding of woody plants with slow growth development ...

Superstrong fusion promoter, fusion method and use thereof

A ultrastrong fusion promoter oms4, its expression vector and its application in transgenic tobacco are disclosed. Said oms 4 promoter is constructed with 4 copied ocs enhensers by cyclically forwardlinking them to the core fragment of mas promoter. The oms4 promoter can be used to replace the 35S promoter in expression vector pBI121 of plant for constructing pOMS4. Its advantage is high expression level of exogenous gene gus in tobacco (increased by 23-47 times).

Method for repairing heavy metal and organic compound contaminated soil using transgenic plant

The invention discloses a method for restoring soil compositely polluted by heavy metals and organics by using a transgenic plant and is technically characterized in that foreign genes which can strengthen the efficiency for restoring the organic pollution of the plant and foreign genes which can improve the tolerance and the accumulation to a plurality of metals of the plant are introduced to the plant so as to establish a transgenic plant which has a better restoring effect on the pollution by the heavy metals and the organics. The method of the invention has a better restoring effect on the polluted soil of the three types as follows: (1) the soil polluted by organic pollutants of halohydrocarbon compounds such as trichloroethylene, dibromethane, and the like; (2) the soil polluted by various heavy metals such as Cd, Cr, Cu, Mn, Zn and Pb; (3) the soil compositely polluted by the heavy metals and the organics. In addition, the method of the invention comprises the procedures of establishing ...

Channel decoding method, device and low-density parity code transcoder

The invention discloses a method and a device of channel decoding and a low density parity check code decoder, which belongs to the coding and decoding technical field. The method of channel decoding includes that: a variable node is initialized, a check node update process is performed according to an initialized result of the variable node; an update process of the variable node is performed according to a result of the check node update process and a modifying function, which is: theta (x)=log(ex+n/ex-1), wherein, n is more than -1 and less than 1; a decoding process is performed according to the initialized result of the variable node and the result of the update process of the variable node. Compared with the prior SPA decoding, the invention can avoid the effect of inaccurate variance estimation of the channel noise to the performance of the SPA decoding to certain degree under the condition of not increasing calculation quantity of decoding, thereby improving the stability of the ...

Method for authenticating green-keeping capacity of maize

The invention relates to a method for authenticating the green-keeping capacity of maize. In the method, the green-keeping capacity of the maize is evaluated according to the dual indexes of the green-keeping degree of three ear leaves and the number of green leaves in the mature period. The simple method for authenticating the green-keeping capacity of the maize by using the obvious correlation between the green-keeping degree of the whole-plant leaves and the green-keeping degree of the three ear leaves and between the green-keeping degree of the whole-plant leaves and the number of the green leaves is applied to the selective breeding of the maize.

Cotton Na+/H+ reverse transport protein gene and its cloning method and use

The present invention relates to the cloning, recombination and salt tolerance analysis of cotton Na+/H+ reverse transport protein GhNHX1 gene, and belongs to the field of molecular biology and biological technology. Total RNA is extracted from cotton leaf treated with 0.4 mol/L NaCl solution and inversely transcribed into cDNA. Facultative primer is used for conventional PCR to obtain intermediate segment, and whole length cDNA is obtained through fast amplification in 3' and 5' ends. The gene is transformed to salt-sensitive yeast mutant to restore its salt tolerant capacity to some degree;and the gene is further transformed to tobacco to obtain transgenic plant capabl eof growing normally in salt concentration of 200 mmol/L. Therefore, the gene is one important salt tolerant gene and may be used in raising the salt tolerant capacity of plant for plantation in saline land.

Soft decision method and apparatus, channel decoding system

The embodiment of the invention discloses a soft-decision method and a device as well as a channel decoding system; the method comprises the steps that: a correlation operation is carried out on the data to be decoded and a plurality of preset NR code words, so as to obtain a plurality of corresponding correlation values from which at least two correlation values are acquired, and the two correlation values include a maximum correlation value and a sub-maximum correlation value; according to the at least two correlation values and the corresponding NR code words thereof, soft-decision information is obtained by combination in proportion. The adoption of the technical proposal has the following advantages: through the soft-decision information calculated and obtained according to the correlation values, the confidence level of a signal can be represented in a more reliable manner in the case that the signal is interfered rather seriously, thereby improving the performance of the decoding ...

Method for separating and purifying honokiol and magnolol by using high speed counter current chromatography

Decoding method and system

The embodiment of the invention discloses a decoding method, a device and a system thereof, pertaining to the communication field and saving the storage resource of an LDPC decoder. The technical proposal provided by the embodiment of the invention is as follows: a decoding method which comprises the steps of numbering data before deinterlacement according to interlacing parameters by a format after deinterlacement so as to obtain serial numbers of data after deinterlacement, calculating the memory address information of deinterlaced data according to the serial numbers after deinterlacement,and decoding the stored deinterlaced data according to the deinterlaced data stored in the memory address information. The embodiment of the invention realizes the deinterlacing function according torelationship between data before and after the interlacement while receiving and storing data so as to save RAM required by the deinterlacement.

Tobacco strong nuclear matrix binding sequence, separating identification method and use thereof

A strong nuclear matrix binding sequence (MAR) of tobacco and its separating and assay method and application are disclosed. A novel strong MAR sequence is separated from the tobacco genom and has the typical characteristics of MAR sequence and the stronger speicfic binding power with nuclear matrix in vitro. Said MAR sequence can be used to construct plant gene expression vector, that is, antrorsly constructing it at both sides of the gene expression cassette of expression vector pBI121 gus. It can increase the average expression level of exogenous gus gene by 5 times, and used in genetic engineering.

Molecular mark closely interlocked with green-keeping main effect QTL of corn leaf blade

The invention relate to a molecular mark closely interlocked with a green-keeping main effect QTL of a corn leaf blade. By constructing a corn molecular mark genetic map and quantitative character site analysis, the molecular mark closely interlocked with the green-keeping main effect QTL of the corn leaf blade is discovered. The molecular mark is applied to corn breeding and resource identification, and can predict the green-keeping performance of germplasm material leaf blades so as to achieve early prediction and screening of the corn leaf blade with the green-keeping performance of and improve the breeding efficiency.

Cotton Na+/H+ reverse transport protein gene and its cloning method and application

Picea wilsonii transcription factor PwHAP5 and coding gene and application thereof

The invention discloses a picea wilsonii transcription factor PwHAP5 and a coding gene and application thereof. The PwHAP5 is protein disclosed in 1) or 2): 1) the protein composed of an amino acid sequence disclosed in a sequence 2 in a sequence table; 2) the protein which is performed with substitution and/ or deletion and/ or adding of one of multiple amino acid residue by the amino acid sequence disclosed in the sequence 2 in the sequence table, is relevant to plant seed germination and is derived by 1). After the coding gene of PwHAP5 protein is guided into arabidopsis, the germination rate of the seed of transgenosis arabidopsis is obviously higher than that of wild arabidopsis in high-salt and wild environment.

Method for remarkably improving transformation efficiency, expression level and genetic stability of exogenous genes in plant

The invention relates to a method for remarkably improving transformation efficiency, expression level and genetic stability of exogenous genes in a plant. A fragment of nuclear matrix combining sequence (MAR) TM6 separated from tobacco by using a nuclear matrix separation method is constructed at two ends of a monocotyledonous/dicotyledonous plant expression vector report gene expression box driven by different promoters and guided into the plant through an agrobacterium-mediated method. In the obtained transgenic plant, the expression quantity of the exogenous genes carrying the TM6 is 10 times higher than that without carrying the TM6, the transformation efficiency of the exogenous genes is improved by 40 percent, and meanwhile, the MAR transgenic plant improves the genetic stability of the exogenous genes in offspring individuals by 30 percent. Meanwhile, the TM6 can be used as an enhancer element for remarkably improving the expression strength of the promoters, but does not change ...

Channel decoding method, device and low-density parity code transcoder

Decoding method and system

The embodiment of the invention discloses a decoding method, a device and a system thereof, pertaining to the communication field and saving the storage resource of an LDPC decoder. The technical proposal provided by the embodiment of the invention is as follows: a decoding method which comprises the steps of numbering data before deinterlacement according to interlacing parameters by a format after deinterlacement so as to obtain serial numbers of data after deinterlacement, calculating the memory address information of deinterlaced data according to the serial numbers after deinterlacement, and decoding the stored deinterlaced data according to the deinterlaced data stored in the memory address information. The embodiment of the invention realizes the deinterlacing function according to relationship between data before and after the interlacement while receiving and storing data so as to save RAM required by the deinterlacement.

Soft decision method and apparatus, channel decoding system

The embodiment of the invention discloses a soft-decision method and a device as well as a channel decoding system; the method comprises the steps that: a correlation operation is carried out on the data to be decoded and a plurality of preset NR code words, so as to obtain a plurality of corresponding correlation values from which at least two correlation values are acquired, and the two correlation values include a maximum correlation value and a sub-maximum correlation value; according to the at least two correlation values and the corresponding NR code words thereof, soft-decision information is obtained. The adoption of the technical proposal has the following advantages: through the soft-decision information calculated and obtained according to the correlation values, the confidence level of a signal can be represented in a more reliable manner in the case that the signal is interfered rather seriously, thereby improving the performance of the decoding device. The embodiment of the ...

SiTAF10 gene for improving drought resistance and salt resistance of millet and application of SiTAF10 gene

The invention provides a SiTAF10 gene for improving drought resistance and salt resistance of millet and application of the SiTAF10 gene. The gene SiTAF10 with a nucleotide sequence shown as SEQ ID NO.1 is separated and cloned from foxtail millet, and fluorescent quantitative expression analysis shows that the gene SiTAF10 participates in various abiotic stresses. After the gene is over-expressed in millet, in drought and salt stress experiments, compared with a wild type strain, the survival rate and the chlorophyll content of a strain with the over-expressed SITAF10 gene are improved by more than two times, and the accumulation of reactive oxygen species (ROS) is remarkably reduced. It is shown that the SiTAF10 gene and the protein coded by the SiTAF10 gene can significantly improve the drought resistance and salt resistance of plants. Therefore, the SiTAF10 gene is a new drought-resistant and salt-resistant gene resource of the millet, plays a positive role in improvement of drought-resistant ...