15-06-2011 дата публикации
Номер: CN0102094090A
Автор:
TING WANG,
SHENGPING ZHANG,
YE LU,
ZHENGAN TIAN,
CHUANGUI WANG,
ZHENGPING DU,
WANG TING,
ZHANG SHENGPING,
LU YE,
TIAN ZHENGAN,
WANG CHUANGUI,
DU ZHENGPING
Принадлежит:
The invention relates to a cholera toxin virulence gene detection kit and a detection method thereof. The kit of the invention contains three pairs of primers which are designed by using vibrio cholera ctxA gene as target gene on the basis of the loop-mediated isothermal amplification technology, namely inner primers FIP/BIP, outer primers F3/B3 and ring primers LF/LB and also contains Bst DNA polymerases, reaction solution, sample pretreatment solution, coloring liquid, stabilizing solution and positive control. The method for detecting the cholera toxin virulence gene comprises the following steps: extracting bacterial DNA, performing the loop-mediated isothermal amplification of the cholera toxin virulence gene and coloring for detection. The kit of the invention has high amplificationefficiency, specificity and sensitivity, low omission ratio and obvious coloring effect and is suitable for the rapid detection of toxigenic vibrio cholera.
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