Production method of benzofuranone

The invention provides a production method of benzofuranone, which is characterized in that o-Hydroxyphenylacetic acid is subject to azeotropic dehydration with a solvent only in the presence of commercial catalyst of cationic resins to generate benzofuranone, wherein the reaction temperature is 60-150 DEG C; the dosage of the catalyst is 0.1-20%; and the solvent accounts for 5-80% of the o-Hydroxyphenylacetic acid. The reacted solvent is no need of special treatment, and the filtered solvent is dehydrated to obtain the benzofuranone with concentration being above 96% in high yield.

Resource use method for waste fermenting liquid of ultra-large fermentation plant

Process for recovering gulonicacid from gulonicacid mother liquor

Preparation for film biological reactor for beer and alcohol production

The present invention relates to a preparation method of film-type bioreactor by uses of an adsorption method. The active materials of diatomaceous earth, active carbon and fibre powder and used as the carrier to support colloid, which is applied to an adherend to make up a film and further this film is fixed in rector according to 0.3-5% of filling ratio. Said preparation method is simple and feasible. The reactor can make fermentation according to the original fermentation process and condition. The utilization of said invented equipment for producing beer can shorten the fermentation period by 20-30%, and the beer yield can be raised by 30-40%. Its energy consumption can be reduced. This technique is specially applicable to the reformation on existent production equipment. Besides, it can improve product quality and raise yield.

Method for deep dehydration of residual activated sludge

The invention relates to a method for deep dehydration of residual activated sludge, particularly to a novel technique for realizing deep dehydration of residual activated sludge by applying waste organic acid mother liquor. The method is as follows: collecting the residual activated sludge which is dehydrated mechanically, adding 20-50% of waste organic acid mother liquor based on weight of the residual activated sludge, mixing the mixture to be slurry, putting the slurry into a reaction kettle and heating to 60-100 DEG C for reaction for 0.5-5h, during which the living biological substances contained in the sludge are decomposed, the protein denatures and the bound water in the sludge changes to be free water and exudes; and after the reaction is finished, transferring the resultants into a mixing pool, adding plant debris and the like which serve as fillers, when the mixture turns to be thick catsup, putting the mixing pool into a drying device, and drying until the water content is ...

Antibiotic soil modifier made by gulconic acid mother liquor and method for preparing same

Method for designing bus integral power supply plugging device

The invention relates to an electric appliance product, in particular to a method for designing a bus integral power supply plugging device. The bus integral power supply plugging device is characterized by comprising end covers (1), a socket (2) and a plug converter (3), wherein the socket (2) in a strip-shaped structure is provided with grooves, and both ends are provided with the end covers (1); conductors in the grooves have the bus integral structure; the plug converter (3) is plugged into the grooves of the socket (2) by a T-shaped contact arm (7) at the bottom end and rotates by 90 degrees; a contact point embedding element (6) in the plug converter (3) is in contact with conducting bars (11) of a fire line and a zero line which are connected with the socket (2) to finish the conductor connection between the socket (2) and the plug converter (3); and the rear parts of the conducting bars in the socket are provided with elastic rubber ropes with circular cross sections. The power ...

Synthesis method of S-metolachlor

The invention discloses a synthesis method of S-metolachlor, comprising the following steps: 1) reacting (R)-methyl lactate or (R)-ethyl lactate with methanesulfonyl chloride to generate (R)-2-(mesyloxy)propionate; 2) reacting the obtained (R)-2-(mesyloxy)propionate with 2-methyl-6-ethylphenylamine to obtain (S)-N-(2-methyl-6-ethyl phenyl) phenylalanine ester; 3) reducing an ester group into corresponding alcohol by a reducing agent; 4) obtaining acyamino alcohol by performing acylation on the alcohol and chloroacetic chloride; and 5) obtaining the S-metolachlor through methylation reaction. The S-metolachlor produced by the synthesis method is low in production cost and good in optical purity, the ee value reaches 90-93%, and the yield is up to 86-89%. The synthesis method is simple to operate and easy to realize industrialization.

Vc two-step fermentation microecological regulation and control technique

The present invention relates to a preparation method of Vc, in particular it is a fermentation process in the production of Vc, and is mainly characterized by that on the basis of original method said fermentation process adopts inoculation of liquid inoculum, number of inoculum is 10 to the power 8-10 to the power 9/ml, two strain ratio (small : big) is 20-30, fermenting for 16-24 hr., regulating pH value to 7.0-8.0, continuing for 5-60 min., returning to original process, fermenting 24-36 hr. and regulating pH 6.8-7.1 until the fermentation is completed. Said invention is simple in operation, has no need of increasing new equipment, and is easy to extensively use.

Atrazine herbicide degradation bacterium preparation making method

The present invention is atrazine herbicide degrading bacterium and the preparation process of the microbial preparation, belongs to the field of microbiology technology, and relates to production ofgreen agricultural product. The preparation process of the microbial preparation of sewer enteri bacillus includes the steps of slant cultivation, shake culture to obtain seed liquid, seed tank culture, and fermentation culture to obtain the preparation. The microbial preparation has atrazine residue lowering rate over 87 %.

Production method of chloromethyl ethyl ether

The invention relates to a method for producing chloromethyl ethyl ether. The chloromethyl ethyl ether with high yield and high purity is made by adding a by-product chlorine hydride generated by production of methyl isocyanate to a mixture of ethanol and paraformaldehyde while stirring for reaction at the temperature of 0-50 DEG C, wherein, the mass ratio of the paraformaldehyde to the ethanol is 1-2:1. In the process of producing the chloromethyl ethyl ether by the method, as the by-product chlorine hydride generated by production of the methyl isocyanate is used, the waste gas from the methyl isocyanate is utilized, which improves environment and lowers cost; in the method for producing the chloromethyl ethyl ether, a little amount of impurities such as the methyl isocyanate, and the like, contained in the chlorine hydride facilitate the reaction and improve the reaction yield.

High-concentration continuous fermentation process of sorbitol

The invention relates to the field of first-step fermentation technology of vitamin C, in particular to a high-concentration continuous fermentation process of sorbitol. The process comprises the following steps of: selecting two fermentation tanks; performing fermentation of low sorbitol concentration in the No.1 tank until the growth and the metabolism of black acetobacter reach peak values; removing a part to serve as inoculation solution; performing the fermentation of low sorbitol concentration in the No.2 tank in which the prepared culture medium with low sorbitol concentration is inoculated; after removing a part of the fermentation liquor from the No.1 tank, adding high-concentration sterile sorbitol into the flow of the fermentation liquor until the final concentration of the sorbitol is 38 percent; increasing ventilation volume, continuously fermenting until the conversion rate of the alditol reaches over 97 percent and ending the fermentation; rapidly introducing the fermentation ...

Method for detecting trypsin inhibitor activity

The invention relates to the enzyme catalysis/enzyme inhibition biotechnological field, in particular to a test method for activity of a trypsin inhibitor. The particular test process is mainly that: a trypsin inhibitor extract and a trypsin action substrate are respectively added into a trypsin reaction system; after reaction for 10 to 15 minutes, reaction stopping solution is added, and then absorbance of the reaction stopping solution in the wavelength of 410 nautical miles is measured; a curve is drafted according to addition and the absorbance of the inhibitors; the activity of the trypsin inhibitor is determined; wherein, the trypsin action substrate is BAPNA solution and the addition is 0.5 to 1.0 milliliters per milliliter trypsin reaction system. The invention has the advantages of simple instruments used, convenient operation and high reaction sensitivity, and is suitable for testing activity of various trypsin inhibitors.

Atrazine herbicide degradation bacterium and preparation method for bacterium preparation of the same

The present invention is atrazine herbicide degrading bacterium and the preparation process of the microbial preparation, belongs to the field of microbiology technology, and relates to production of green agricultural product. The preparation process of the microbial preparation of sewer enteri bacillus includes the steps of slant cultivation, shake culture to obtain seed liquid, seed tank culture, and fermentation culture to obtain the preparation. The microbial preparation has atrazine residue lowering rate over 87 %.

Gulonic acid mash quick-hyperfiltration fermentation controlling method

The invention relates to a two-step fermentation technique for Vitamin C, in particular to a fermentation control method of gulonic acid fermented liquor ultrafiltration. The Vitamin C is fermented and therefrom gulonic acid fermented liquor is obtained. Then bacteria are discarded by centrifugation. The upper clear liquid is added with alkaline or acid methanol solution and conductance of the medium is adjusted to 1600-1800mS. The ultrafiltration process of the fermented liquor is finished in a temperature between 40 DEG C and 45 DEG C and 1.8-2.4 times of a normal atmosphere through interception of ultrafiltrating film with molecular weight of 30,000-100,000 daltons. The invention can reduce 30-70 percent of fermented liquor ultrafiltrating time and 30-50 percent of filtrating film washing times and improve 15-20 percent of equipment using rate and meanwhile dramatically save materials, power, time and labor caused by washing films.

Fermentation process for high suspension beer yeast in conventional equipment

The present invention relates to a high-speed production process used in traditional fermentation of brewing method. After having been condensed and cultured the Abort yeast is added in 11 deg.B wort to implement deep-fermentation according to the conventional process, and when the wort sugar concentration is reduced to 3.0-2.5 deg.B, one is passed through the porcess of after-fermentation, at the same time the high kranse beer whose weight is 5-20% of total weight and sugar concentration is 6.5-8.0 deg.B is added, the pH value is regulated to 4.6-4.2 to make the beer ripe.

Method for producing amino acid fertilizer by using Vc fermentation mash

The invention belongs to a field in which waste materials are used to produce manure, in particular to a method which utilizes Vc fermented liquor to produce amino acid manure, and by applying the method, amino acid liquor manure with low production cost and high efficiency can be produced. In the preparation process of the amino acid liquor manure, the ultrafiltered Vc fermented liquor is collected, under a certain condition, the protein in the Vc fermented liquor is hydrolyzed into amino acid by utilizing alkali, and an appropriate amount of microelements (Fe, Zn, Mn, Mo and B) is supplemented in the Vc fermented liquor, so that the amino acid liquor manure with the weight content reaching over ten percent is produced. According to actual planting circumstances, the invention can be applied in an appropriate amount by the methods, such as spraying, irrigating, etc. and can notably increase yield and effectively prevent diseases of crops.

Method for producing mycoprotein and polypeptide organic fertilizer by fermented waste of vitamin C

The invention relates to a method for producing mycoprotein and polypeptide organic fertilizer by waste fermented mash and gulonic acid mother solution formed in the vitamin C production technology, which belongs to the field of biotechnology and waste resource utilization technology, and comprises the following steps: adding 5 to 20 parts of gulonic acid mother solution in 100 parts of waste vitamin C fermented mash protein, adjusting pH value to 2.0 to 5.0, and hydrolyzing for 0.5 to 12 hours under the temperature of 40 to 70 DEG C. On the one hand part of protein in fermented mash protein is hydrolyzed to become polypeptide and amino acid to dissolve in water, through adjusting pH value and appropriate concentration, right amount of trace element is added, obtaining polypeptide organicfertilizer, on the other hand the rest protein in mash protein is precipitated, and the precipitated protein is made into mycoprotein through filter-pressing, drying and smashing. The invention recycles ...

Method for producing amino acid fertilizer by using Vc fermentation mash

Production method of chloromethyl ethyl ether

The invention relates to a method for producing chloromethyl ethyl ether. The chloromethyl ethyl ether with high yield and high purity is made by adding a by-product chlorine hydride generated by production of methyl isocyanate to a mixture of ethanol and paraformaldehyde while stirring for reaction at the temperature of 0-50 DEG C, wherein, the mass ratio of the paraformaldehyde to the ethanol is 1-2:1. In the process of producing the chloromethyl ethyl ether by the method, as the by-product chlorine hydride generated by production of the methyl isocyanate is used, the waste gas from the methyl isocyanate is utilized, which improves environment and lowers cost; in the method for producing the chloromethyl ethyl ether, a little amount of impurities such as the methyl isocyanate, and the like, contained in the chlorine hydride facilitate the reaction and improve the reaction yield.

2-phenyl-4,6-dichloro pyrimidine preparation method

The invention discloses a production method for 2-phenyl-4, 6-dichloropyrimidine, which includes the following steps: 2-phenyl-4, 6-dihydroxy pyrimidine is added into an aprotic polar organic solvent, and under the existence of tertiary amine organic base and the temperature of 30 to 155 DEG C, phosgene is pumped in the course of mixing and reacts with the mixture for 4 to 16 hours; nitrogen is used for removing the excess phosgene after reaction; the mixture is filtered after being cooled, and the filtrate is washed by water to be neutral; the solvent is recovered by distillation or vacuum distillation after being dried by desiccant, and 2-phenyl-4, 6-dichloropyrimidine is obtained. The production method has the beneficial effects that: (1) the method for producing fenclorim uses tertiary amine catalyst as the catalytic, which improves the reaction process. (2) As the method for producing fenclorim adopts phosgene to replace phosphorus oxychloride, the discharging of phosphorous wastewater ...

Method for preparing methyl parathion degradation bacterium and enzyme preparation thereof

Consortive process for culturing bacillus thuringiensis

A consortive method for preparing bacillus thuringiensis incldues such steps as mixing bacillus thuringiensis with oxidizing gluconic acid bacillus, culturing in the culture medium containing sorbose(1-2%) until that their number ratio is 1:10-20, inoculating to fermenting culture meidum containing sorbose (6-14%), and aerobic fermenting. Its advantages are high conversion of 2-keto-L-colombic acid and high yield of bacillus thuringiensis.

Method for producing mycoprotein and polypeptide organic fertilizer by fermented waste of vitamin C

The invention relates to a method for producing mycoprotein and polypeptide organic fertilizer by waste fermented mash and gulonic acid mother solution formed in the vitamin C production technology, which belongs to the field of biotechnology and waste resource utilization technology, and comprises the following steps: adding 5 to 20 parts of gulonic acid mother solution in 100 parts of waste vitamin C fermented mash protein, adjusting pH value to 2.0 to 5.0, and hydrolyzing for 0.5 to 12 hours under the temperature of 40 to 70 DEG C. On the one hand part of protein in fermented mash protein is hydrolyzed to become polypeptide and amino acid to dissolve in water, through adjusting pH value and appropriate concentration, right amount of trace element is added, obtaining polypeptide organic fertilizer, on the other hand the rest protein in mash protein is precipitated, and the precipitated protein is made into mycoprotein through filter-pressing, drying and smashing. The invention recycles ...

Active chitosan food preservative and its prepn. method

The present invention relates to natural food preservative which is made from high molecular chitosan with deacetyl degree higher than 80% by 10-100 KGy cobalt 60 gamma radiation treatment to make its molecular weight reduced to 30-150 thousand dalton. After purification, it can be added to meat, bean, fruit products at the quantity of 0.02-0.1 gm. per kilogram food. Advantages, low cost, no toxic side effect, can enhance immunity regulating function of human body.

Applications of companion fungus activity extracellular fluid preparation in two-step fermentation of Vc

The invention relates to the field of second-step fermentation of vitamin C, particularly discloses applications of a companion fungus activity extracellular liquid preparation in two-step fermentation of Vc, comprising the following applications: (1) selecting a companion fungus in two-step fermentation of Vc; inoculating the companion fungus into a beef extract peptone culture medium and the like; carrying out shaking culture at the temperature of 33 DEG C until the growth stage is stable; collecting fermentation mash; filtering abandoned mycelium by a 0.45 mu m filter membrane; ultrafiltering filtrate liquor by the filter membrane the cut-off molecular weight of which is 3-5 Dalton to concentrate into 10-20mg/ml protein, so as to prepare the companion fungus activity extracellular liquid preparation; carrying out sterile separation packing, and preserving at the low temperature; and (2) in the two-step fermentation of Vc, when the fermentation enters middle stage or later stage, respectively ...

Resource use method for waste fermenting liquid of ultra-large fermentation plant

The invention belongs to the field of the agricultural utilization of fermented waste mash, in particular to a resource utilization method of the waste mash of a super large fermenting plant, which is a method using vitamin C fermented waste mash to produce bacterial protein feed and amino acid compound fertilizer. The making processes of the bacterial protein feed are that the vitamin C fermented waste mash is collected, cells and high molecular weight protein in the vitamin C fermented waste mash are condensed and precipitated by using dilute alkali, thereby the bacterial protein feed whichcomprises more than 55% protein by weight is separated, pressed into a cake and dried. The preparation processes of the amino acid compound fertilizer are that vitamin C fermented mash which is condensed is collected, the protein in the vitamin C fermented mash is hydrolyzed into amino acids by using alkali, through supplying proper trace elements, amino acid liquid fertilizer which comprises more than ...

Two-step fermentation method for preparing VC precursor

Method for deep dehydration of residual activated sludge

The invention relates to a method for deep dehydration of residual activated sludge, particularly to a novel technique for realizing deep dehydration of residual activated sludge by applying waste organic acid mother liquor. The method is as follows: collecting the residual activated sludge which is dehydrated mechanically, adding 20-50% of waste organic acid mother liquor based on weight of the residual activated sludge, mixing the mixture to be slurry, putting the slurry into a reaction kettle and heating to 60-100 DEG C for reaction for 0.5-5h, during which the living biological substancescontained in the sludge are decomposed, the protein denatures and the bound water in the sludge changes to be free water and exudes; and after the reaction is finished, transferring the resultants into a mixing pool, adding plant debris and the like which serve as fillers, when the mixture turns to be thick catsup, putting the mixing pool into a drying device, and drying until the water content is 10 ...

Method for producing substituted dichloropyrimidine

The invention relates to a method for producing 2-subtituted-4,6-dichloropyrimidine, which comprises the following steps of: suspending 2-substitued-4,6-dihydroxypyrimidine in an aprotic polar organic solvent; introducing phosgene into the suspension during stirring in the presence of tertiary amine organic alkali at 30 to 155 DEG C for reaction for 4 to 16 hours; after the reaction is finished, removing excessive phosgene with nitrogen; cooling the reaction product, filtering the product and washing the filtrate till the filtrate is neutral; and drying the filtrate by a drying agent, reclaiming the solvent through distillation or distillation under reduced pressure, and obtaining the 2-subtituted-4,6-dichloropyrimidine. The method for synthesizing the 2-subtituted-4,6-dichloropyrimidine has the advantages that: (1), a tertiary amine catalyst is used to catalyze the reaction and accelerate the reaction; (2), in a reaction process, the phosgene is used as a substitute for phosphorus oxychloride ...

Method for improving fermentation efficiency by using fermentation tail gas

The invention relates to a method for improving fermentation efficiency by using fermentation tail gas, belonging to the field of fermentation tail gas recovery. By adopting the method, the fermentation tail gas can be recycled, the energy is saved, the emission is reduced and the fermentation efficiency is improved. The method comprises the following steps that: tail gas produced in the process of aerobic fermentation is collected in a buffer tank and is guided into a special auxiliary compression pipeline communicated with a fermentation tank after the tail gas is pressurized by an air blower. When the fermentation is active and the oxygen demand is increased, the valve of the fermentation tank is opened and the tail gas is filled in the fermentation tank through a gas distributor arranged at the middle part of the fermentation tank, so oxygen is supplemented to the upper part of the fermentation tank, the overturning effect of fermentation liquid in the fermentation tank is enhanced, ...

2-phenyl-4,6-dichloro pyrimidine preparation method

The invention discloses a production method for 2-phenyl-4, 6-dichloropyrimidine, which includes the following steps: 2-phenyl-4, 6-dihydroxy pyrimidine is added into an aprotic polar organic solvent, and under the existence of tertiary amine organic base and under the temperature of 30 to 155 DEG C, phosgene is pumped in the course of mixing and reacts with the mixture for 4 to 16 hours; nitrogen is used for removing the excess phosgene after reaction; the mixture is filtered after being cooled, and the filtrate is washed by water to be neutral; the solvent is recovered by distillation or vacuum distillation after being dried by desiccant, and 2-phenyl-4, 6-dichloropyrimidine is obtained. The production method has the beneficial effects that: (1) the method for producing fenclorim uses tertiary amine catalyst to play the catalytic action, which improves the reaction process. (2) As the method for producing fenclorim adopts phosgene to replace phosphorus oxychloride, the discharging of ...

Improved solid double-layer flat plate method for quick high-flux sifting motion of polycyclic aromatic hydrocarbon degradation bacterium

The invention relates to an improved solid double-layer flat plate method for the rapid high -throughput screening of PAHs degrading bacteria, which pertains to the field of the bioremediation technology of contaminated soil and is applicable to the excavation of bioremediation microbial resources of the PAHs contaminated soil. The adopting solid double-layer flat plate method is that a lower layer culture medium is an ordinary inorganic slat agar culture medium which is added with a variety of vitamins and trace elements, an upper layer culture medium is an inorganic salt low-melting point agarose culture medium which takes the PAHs as a sole carbon source; the low-melting point agarose, which is selected to replace the ordinary agar to produce the upper layer culture medium, can preventthe degradation or volatilization due to the high temperature when the PAHs is added on the one hand and allow the degradation dark circle to be more clear to be identified on the other hand. The method ...

一种应用Vc二步发酵弃渣生产的防病菌肥

A disease-preventing bacterial fertilizer is prepared from the waste generated by two-step fermentation of VC through mixing with water, stirring, ultrasonic treating, heating, alkaline hydrolyzing or enzymolyzing, and adding the superfiltered substance of said waste, trace elements and filler. Its advantages are high effect and low cost.

Screening method for phophonomycin biological conversion strain

The present invention relates to biological converting and biological catalyzing technology, and is especially screening method of phophonomycin biologically converting strain. The present invention cultures the strain to be detected in cylindrical agar block as solid culture medium, utilizes colibacillus as indicating bacterium for biological identification, and sets the agar block backwards on biological identification disc to screen phophonomycin converting strain. According to that different strains to be screened in conversion capacity and grown on isometric agar block with the same amount of precursor will produce different phophonomycin concentrations and show bacteriostasis rings of different sizes on the indicating bacterium plate, the strain with phophonomycin converting predominance may be sorted out. The method is simple and suitable for initial screening of antibiotic converting bacteria.