25-04-2023 дата публикации
Номер: CN116008369A
Принадлежит:
The invention discloses an electrochemical detection kit for high-sensitivity detection of AFB1 based on an eRAFT polymerization signal amplification strategy and application. The kit comprises a gold electrode, Aptamer, FcMMA, MCH, CPAD, C6H4BBrF4N2, Ab, DMF, KPF6, EDC and NHS. An aptamer with one end modified with sulfydryl is connected to a gold electrode through an Au-S bond, so that the aptamer specifically captures AFB1 in a sample, Ab * specifically recognizes the AFB1, then eRAFT reaction is started under the action of constant negative potential, and a long-chain polymer formed by a large number of electroactive probes FcMMA is generated on the surface of the electrode. Under the optimal condition, the current intensity of the method and the logarithm of the AFB1 concentration have a good linear relationship, the linear range is 2 * 10 <-4 > to 2 * 10 < 2 > ng/mL, and the limit of detection (LOD) is 37.34 fg/mL (S/N = 3). The kit has the characteristics of high sensitivity, good ...
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