27-01-2010 дата публикации
Номер: CN0101633948A
Автор:
LIU DONGMEI,
YU YIGANG,
LIANG JIEYING,
WU HUI,
WU ZUXING,
CHEN ZIJIAN,
LI XIAOFENG,
TANG YUQIAN,
DONGMEI LIU,
YIGANG YU,
JIEYING LIANG,
HUI WU,
ZUXING WU,
ZIJIAN CHEN,
XIAOFENG LI,
YUQIAN TANG
Принадлежит:
The invention discloses a kit test method of beta-lactam antibiotics residue in milk, comprising the following steps: performing once activation to bacillus stearothermophilus, then culturing the activated bacillus stearothermophilus in a production culture medium at 50-65 DEG C for 24-36h while stirring in a rotation speed of 100-150rpm, centrifugalizing to collect bacteria mire, adding agarose bromcresol purple solution, mixing evenly to ensure the number of spore in each kit reach 1.00*10<7>-2.50*10<8>cfu, subpackaging the spore in porous reaction vessels at 60-70 DEG C, covering aluminum-plastic film, placing upside down, sealing, placing the vessels in aluminum-plastic packages and storing in dark place while keeping from wet. The kit has the advantage of easy operation, high sensibility, good repeatability, low cost and high throughput, can be stored at 4-8 DEG C for 12-18 months and can be used to test beta-lactam antibiotics residue in milk.
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