17-02-2022 дата публикации
Номер: US20220049018A1
Автор:
Bedzyk William,
Dominowski Allison,
Draghici Bogdan,
Lebedeva Tatiana,
Mareeva Tatiana,
Morjana Nihmat,
Sharma Manoj,
Smirnov Yuriy,
Teng Zhu,
Yau Heungyeung,
Zheng Yi Feng
Antibodies that specifically bind naloxone or naltrexone are disclosed. Also disclosed are conjugates used in the production of the antibodies, as well as methods of producing the antibodies. Also disclosed are methods of using the antibodies in direct assays for naloxone or naltrexone. Further disclosed are methods of reducing naloxone or naltrexone interference in opiate assays using the antibodies. 2. An antibody or functional fragment thereof which can specifically bind to naloxone and/or naltrexone , wherein the antibody comprises:(1) heavy chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:2, 3, and 4, respectively, and light chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:6, 7, and 8, respectively;(2) heavy chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:22, 23, and 24, respectively, and light chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:26, 27, and 28, respectively;(3) heavy chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:42, 43, and 44, respectively, and light chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:46, 47, and 48, respectively;(4) heavy chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:62, 63, and 64, respectively, and light chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:66, 67, and 68, respectively;(5) heavy chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:82, 83, and 84, respectively, and light chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:86, 87, and 88, respectively;(6) heavy chain variable region CDR1, CDR2, and CDR3 having the amino acid sequences of SEQ ID NOS:102, 103, and 104, respectively, and light chain variable region CDR1, CDR2, and ...
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