29-08-2019 дата публикации
Номер: US20190265251A1
Принадлежит:
A proteomic expression platform to identify age-related sepsis risk is disclosed using patients with an intra-abdominal infection. A semi-quantitative plasma proteomics workflow was applied which incorporated tandem immuno affinity depletion, iTRAQ labeling, strong cation exchange fractionation, and nanoflow-liquid chromatography coupled to high resolution mass spectrometry. A protein profile was determined that exhibit statistically significant differences in expression levels amongst patients with severe sepsis as a function of age. Representative pathways that are differentially-expressed include, but are not limited to, acute phase response, coagulation signaling, atherosclerosis signaling, lipid metabolism, and production of nitric oxide/reactive oxygen species. 1. A method for treating sepsis development , comprising: i) at least one biological sample derived from an elderly patient suspected of having an intra-abdominal infection;', 'ii) at least one peptide isolated from said at least one biological sample, wherein said at least one isolated peptide comprises at least one isobaric tag for relative and absolute quantitation (iTRAQ) reporter ion;', 'iii) a system comprising a liquid chromatography column and a mass spectrometer;', 'iv) a control proteomic pathway expression profile; and', 'v) a sepsis proteomic pathway expression profile;, 'a) providing;'}b) contacting said at least one iTRAQ-peptide with said system to create at least one iTRAQ-peptide analysis spectrum;c) processing said at least one iTRAQ-peptide analysis spectrum to create a patient proteomic pathway expression profile,d) comparing said patient proteomic pathway expression profile to said control proteomic pathway expression profile and said sepsis proteomic pathway expression profile wherein at least one protein pathway of said patient proteomic pathway expression profile has an overexpression ratio when compared with said control proteomic pathway expression profile and does not have an ...
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