БИОРЕАКТОР ДЛЯ ВЫРАЩИВАНИЯ КЛЕТОК

Изобретение относится к медицине и биологии. Биоректор содержит сосуд, состоящий из цилиндрической средней части и герметично связанных с ней верхней крышки и дна, выполненного в виде нижней крышки, и имеющих по меньшей мере одно отверстие для подвода или отвода питательной среды или кислорода. Указанные крышки имеют кольца с резьбой на внутренней поверхности, а цилиндрическая средняя часть сосуда - наружную резьбу для образования герметичного резьбового соединения с кольцами крышек. Изобретение обеспечивает удобство обслуживания биореактора и возможность извлечения выращенных клеток без их повреждения. 9 з.п. ф-лы, 13 ил.

БИОЛОГИЧЕСКИЙ ИНДИКАТОР РАЗДЕЛЬНОГО ТИПА

Изобретение относится к микробиологии и может быть использовано для контроля стерилизации. Биологический индикатор раздельного типа содержит емкость с носителями тест-организмов и емкость с питательной средой-индикатором. Каждая емкость герметично закрыта крышкой, выполненной в виде мембраны, которая имеет форму петли. Внутренняя часть мембраны соединена герметично по периметру с торцевой стенкой емкости, а ее наружная часть имеет свободный конец, выступающий за боковую стенку емкости. В емкости для питательной среды размещено уплотнение, примыкающее к внутренней части мембраны. В крышке емкости с тест-организмами установлен биофильтр. При использовании биологического индикатора предлагаемой конструкции не требуется соблюдения асептических условий для переноса тест-организма в питательную среду. Полностью исключается контаминация тест-организмов. В значительной степени повышается качество контроля режима стерилизации изделий. Исключается дегидрация питательной среды-индикатора, которая ...

МОДУЛЬ ПЕРЕМЕЩЕНИЯ КОНТЕЙНЕРОВ В КАМЕРЕ ДЛЯ ИНКУБАЦИИ БИОЛОГИЧЕСКИХ ОБРАЗЦОВ

Изобретение относится к медицинской технике, а именно к устройству для инкубации образцов в контейнерах, например в чашках Петри, и получения цифровых изображений результатов посева. Модуль перемещения контейнеров в камере для инкубации биологических образцов с окном для загрузки и выгрузки контейнеров содержит два 3-координатных манипулятора с захватом для контейнера, где каждый из манипуляторов содержит колонну вертикального перемещения захвата, горизонтальные направляющие и поворотный стол, снабженные приводами перемещения. При этом манипуляторы расположены один над другим с возможностью перекрытия рабочего хода друг друга с обеспечением доступа захвата каждого манипулятора к контейнеру, размещенному в рабочем объеме камеры инкубации у окна для загрузки и выгрузки контейнеров, и снабжены датчиками «красной зоны», выполненными с возможностью обнаружения присутствия захвата манипулятора в «красной зоне», расположенной вокруг окна для загрузки и выгрузки контейнеров, доступной для обслуживания ...

Автомат для розлива плотных сред в чашки Петри

Камера для хемотаксисного разделения популяции микроорганизмов

Изобретение относится к устройствам для хемотаксисного разделения популяций микроорганизмов при определении их класстерной гетерогенности. Цель изобретения - повышение точности и полноты разделения популяции микроорганизмов при анализе их класстерной гетерогенности и выделения таким образом наиболее активных штаммов. Камера содержит чашку Петри 1, вкладыш в виде круглой пластины 2, имеющий сверху центральную лунку 3 для инокулята, периферийные лунки 4 и 6 для исследуемых образцов и каналы 5 и 7 для хемотаксисной жидкости. Все каналы имеют одинаковую длину. Камера обеспечивает исследование различных популяций микроорганизмов и позволяет выявлять и характеризовать штаммы по их хемотаксисной подвижности. 2 ил.

VORRICHTUNG ZUR KULTIVIERUNG VON MIKROORGANISMEN

KULTIVATIONSGEFAESS FUER MIKROORGANISMEN, ZELLEN UND GEWEBE

CULTURE MEDIUM SUPPORT SYSTEM

Container handling device

A device for handling and removing lids from petri dishes D comprises an elongate handle 10 having a head 11 at one end defining a downwardly-facing mouth for receiving the dish. The handle comprises a pair of arms 10a, 10b provided with respective jaw members 12a, 12b on opposite sides of the mouth at the upper end thereof for engaging a closure C of the dish D. A head member 13 comprises fingers 17a, 17b, on opposite sides of the mouth at the lower end thereof for engaging a base B of the dish D. In use, the device holds the dish D such that the closure C remains fitted to the base B. The device can move the dish D from one position to another without any risk of contamination. Once at the desired position, the handle 10 can be squeezed to open the jaws 12a, 12b to disengage the base B from the device whilst retaining the closure C in-situ. In this manner the base B is released under the influence of gravity downwardly out of the mouth, such that the closure can be separated from the ...

RECEPTACLE HAVING A DISTENDABLE SIDEWALL

... 1379506 Micro-organism sampling device BAXTER LABORATORIES Inc 23 May 1972 [25 May 1971] 24158/72 Heading C6F A test device useful for determining the presence of micro-organisms on a flat surface includes a shallow plastics receptacle 15, Fig.3, containing gelled culture medium, and a resiliently flexible side wall 18 permitting distension thereof when the top of the side wall is pressed against the flat surface so that the medium is brought into contact with the flat surface. The top portion may be formed as an out-turned rolled rim, as shown in Fig.3, or include upwardly extending and distendible tabs. Alternatively, the side wall itself may be provided with convolutions which yield under stress. The device may have a cover with depending lugs or a downwardly depressed skirt 20, Fig.5.

Sterile, disposable culture vessels for the rapid growth of bacteria

A METHOD FOR THE DETERMINATION OF MICROBE CONCENTRATIONS BY MEANS OF A PLATING METHOD AND DISHES USED THEREIN

Bacterial culture vessel having a lid able to hold encapsulated culture supplements

A bacterial culture vessel is made of plastics to be economically disposable. It has a container 2 with internal fins 7 and a snap-on lid 3 . The container 2 and lid 3 are packaged separately in a sterile manner, and the container can be prepacked with dehydrated culture medium 8 . Conveniently it will stack with other packaged containers. Part of the lid is a blister-pack containing supplements 5 . With the lid in place, these can be selectively released into the container by locally pressing the top of the lid 3 . The lid also has a closable aperture 4 for introducing bacterial inoculum and for aeration. The culture vessel can also adapt, via screw-thread 9 , into a base 1 , allowing it to be held 6 onto a moving platform whilst culturing is in progress.

Culture device for sampling and/or counting micro-organism

PRODUCTS FOR THE GAS EXCHANGE AND THE SEPARATION OF BIOLOGICAL MATERIALS OR THE SEPARATION FROM MATERIAL MIXTURES AND USES OF NANOCOMPOSITES

PETRI PLATE

Multiple incubator for biological material

Inkubator (1) für biologisches Material mit wenigstens zwei in einem Gehäuse (2) angeordneten Inkubationskammern (3) und einer im Gehäuse, vorzugsweise zwischen den Inkubationskammern (3), angeordneten Begasungseinheit (4), aus der Nährgas in das Innere des Gehäuses (2) strömen kann, wobei jeder Inkubationskammer (3) eine im Gehäuse (2) angeordnete Austrittsöffnung (5) zugeordnet ist, aus der von der Begasungseinheit (4) ausströmendes Nährgas aus dem Inneren des Gehäuses (2) entweichen kann.

A FERTILE SOIL EXHIBITING PETRI PLATE

ANORDNUNG UND VERFAHREN ZUM NACHWEIS VON MIKROORGANISMEN IN EINEM KULTURGEFÄSS

The invention relates to an arrangement, in particular hybridization arrangement (1), for specifically detecting microorganisms (2) in a culture vessel (3) with at least two regions, where in at least one first region (4) prokaryotic cells can be cultured and in one at least further region (5) capture molecules (6) for specifically detecting microorganisms (2) which can be cultured in the at least first region (4) are immobilized, and to a method for specifically detecting microorganisms (2) with an arrangement, in particular hybridization arrangement (1), in a culture vessel (3) with at least two regions by hybridization involving the following steps: a) transferring cells or cell constituents of the microorganisms (2) with target molecules from a first region (4), where prokaryotic cells grow, to the at least further region (5) where capture molecules (6) are immobilized, b) binding the target molecules to the capture molecules (6), c) labelling the bonded target molecules and d) detecting ...

POOR COPY CULTURAL PHOTOGRAPHY MODULE.

WEGWERFKULTURSCHALE WITH RIDGES.

CARTRIDGE ARRANGEMENT FOR THE ADMISSION OF BOXES O.DGL., IN PARTICULAR PETRI PLATES

EQUIPMENT FOR THE DETERMINATION OF THE NUMBER OF MICROORGANISMS IN AIR AS WELL AS A PROCEDURE FOR THE ENTERPRISE OF THIS EQUIPMENT

GENERATION OF MICROAEROPHILLIC ATMOSPHERE

Observation device

... [Problem] To provide an observation device with which it is possible to obtain an excellent optical image by keeping contrast constant, while rendering unnecessary an operation for adjusting the distance from a phase-contrast microscope to an object to be observed. [Solution] This observation device 10 comprises a microscope 12. The microscope 12 comprises: an illumination light source 50; an illumination optical system 51; and an observation optical system 52 that forms an optical image of a to-be-observed object 28 from reflection light 62 obtained by illumination light 61 being reflected by the to-be-observed object 28. The observation optical system 52 comprises a phase plate 57 which, among the reflection light 62, changes the phase of direct reflection light 62A reflected by a reflection surface 49a. Both the illumination optical system 51 and the observation optical system 52 are positioned below the to-be-observed object 28. The observation device 10 comprises: an imaging element ...

Method for determining the interaction between keratinocytes and neurons

A micro-bubble plate for patterning biological and non-biological materials

Systems and methods for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiments of the present invention include a method for placing materials on the plate at predetermined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micromechanical structures where the structures may be formed from living or nonliving material, organic or inorganic, and the like.

Apparatus for culturing organism and culture method

Injection moulding articles made of metallocene and polypropylene

Culture medium dispenser and dispensing method

Method for carrying out a biochemical protocol in continuous flow in a microreactor

Automated selection of microorganisms and identification using MALDI

A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after ...

Apparatus and method for growing anaerobic microorganisms

DEVICE FOR RAISING OR CULTIVATING CELLS IN A CONTAINER-LIKE RECEPTACLE

A device for raising or cultivating cells in a container-like receptacle, comprises a base and at least one lid. The at least one lid is connected to the receptacle in a pressure--tight manner. The receptacle or the lid is provided with at least one connector drilling for the introduction and/or extraction of culture medium and/or oxygen.

RECIPIENT POUR L'ETUDE DE LA CROISSANCE DES BACTERIES

COMPOSITIONS AND METHODS FOR CULTURING MICROORGANISMS REQUIRING SPECIAL GASEOUS ENVIRONMENTS

MINIATURIZED YEAST IDENTIFICATION SYSTEM

WELL PLATE

The invention relates to a perforated plate (11) comprising a plate main body (12) and at least one depression (14) in an upper side (13) of the plate main body (12). An upwardly open annular channel (15) is formed in the at least one depression (14), which is delimited on the inner circumference thereof via a closed circumferential wall (16). A horizontal outer circumference of the circumferential wall (16) decreases from bottom to top up to an upper edge (22) of the circumferential wall (16). Within the horizontal outer circumference of the circumferential wall (16) on the upper edge (22), at least two retaining elements (24) are connected upwardly to the upper edge (22) of the circumferential wall (16). The at least two retaining elements (24) are at a free horizontal distance (28) to one another, and at least one of the at least two retaining elements (24) is supported elastically on the plate main body (12) in the horizontal direction.

BIOLOGICAL CHAMBER APPARATUS

MICROELECTROMECHANICAL DEVICES USEFUL FOR MANIPULATING CELLS OR EMBRYOS, KITS THEREOF, METHODS OF MAKING SAME, AND METHODS OF USE THEREOF

The present invention relates generally to microelectromechanical systems (MEMS) devices for the manipulation of cells or groups of cells, such as oocytes, embryos, and sperm. In particular, the present invention relates to Cell Labeling MEMS devices (2F), Microinjection MEMS devices, IntraCytoplasmic Sperm Injection ("ICSI") MEMS devices, Zona Coring MEMS devices, Enucleation MEMS devices, Enucleation/Nuclear Transfer MEMS devices, and Cytoplasmic Transfer MEMS devices. The present invention also relates to kits containing the MEMS devices of the present invention.

SAMPLE CONTACT PLATE WITH LATCHABLE COVER

Contact plates are provided including a base member and a lid. The base member has a sidewall extending circumferentially around a peripheral edge portion thereof to define a sample receiving area. The lid has a sidewall extending circumferentially around a peripheral edge portion thereof. A plurality of ribs extend from an inner surface of the sidewall of the lid. The ribs are configured to provide an interference fit engagement between the sidewall of the base member and the sidewall of the lid when the lid is attached to the base member.

CULTURE DISH AND BIOREACTOR SYSTEM

... ²²²A bioreactor (10) has a base (12) for defining a well (14), a dish (24) with a ²membrane (20) suspended in the base (12), and a lid (26) with gas permeable ²membranes (32) and self-sealing ports (28,30).² ...

DEVICE FOR QUANTIFYING BIOLOGICAL MATERIAL

DEVICE AND METHODS FOR PRODUCTION OF CELL AGGREGATES

A microwell device comprises a fluid vessel having a bottom region. A microwell plate is at the bottom region. The microwell plate comprises an upper region defining an upper plane. A plurality of microwells extend downwardly from the upper plane. Each of the microwells comprisesan axis extending perpendicularly to the upper plane. Each of the microwells comprises a sidewall. The sidewall of at least one of the microwells has at least one wall component extending inwardly towards the axis.

SYSTEM FOR AUTOMATED EXPLANT PREPARATION AND METHOD OF USE

A system and method for the automated or semi-automated preparation of explants for transformation and transgenic engineering.

CONTAINER ASSEMBLY WITH LID KEY MEMBERS AND BASE LATCH MEMBERS THAT PERMIT SECUREMENT/DETACHMENT WITHOUT ROTATIONAL FORCE

A container assembly such as a petri dish or a contact plate for use as a sampling device for microorganisms includes a base member, a lid and a locking mechanism that provides a secure locking engagement between the lid and the base member. The locking mechanism is designed such that does not lock except upon application of a specific intentionally applied compressive force, and which may be readily disengaged from locking engagement without the need for a rotational movement or torsional force.

OXYGEN SCAVENGING SYSTEM FOR ANAEROBIOSIS

DISPOSABLE CULTURE DISH WITH REINFORCEMENT RIBS

DISPOSABLE PETRI DISH WITH REINFORCEMENT RIBS

A Petri dish is disclosed in which the flat bottom is provided with a plurality of preferably radial ribs. The upwardly projecting portions of the ribs do not project more than about 0.050 inches above the upper surface of the bottom. Preferably, the ribs do not project more than about 0.030 inches above the upper surface of the bottom. The thickness of the bottom portion other than at the ribs is not greater than about 0.030 inches. Preferably, this thickness is not greater than about 0.025 inches. In the preferred embodiment, there are at least three radial ribs. Preferably, there are four ribs which are spaced 90 degrees from each other. A lid having the same basic structure may also be produced. The ribs need not necessarily be radial, nor need they necessarily be straight, but could be curved. Also, the ribs need not necessarily be continuous nor need they necessarily run all the way from the centre to the side wall or from side wall to side wall. The ribs could project downwardly ...

MULTIPLATE SUBCULTURE SOLID MEDIA DEVICES

A multiplate subculture solid media device is provided with a connection for internal flow communication with a liquid medium culture bottle. The device includes a large transparent screw cap on one face thereof to gain easy access to the plurality of media plates for direct examination and sample taking through the growth period. The cap may transparent and of a size allowing continuous examination during the growth period without any opening. Alternatively, the cap internal surface may include the solid media plates so that culture growth activity may be directly observed by easy removal of the cap. As a further feature of the invention, the screw cap may include an integral magnifying lens for enhancing the continuous examination of culture growth.

APPARATUS AND METHOD FOR REARING NEMATODES, FUNGI, TISSUE CULTURES AND THE LIKE, AND FOR HARVESTING NEMATODES

... 2078986 9115569 PCTABS00007 Entomopathogenic nematodes are cultured in a culture vessel comprising a tray (10) with side walls (12) and an overlapping lid (11), also with side walls (13). A gasket (14) of polyether polyurethane foam is positioned around the inside edge of the lid. In the preferred vessel, stop members (16), mounted on the inside faces of the walls of the tray, prevent forced movement of the lid towards the tray when flanges (15) on the lid bear against the stop members. Such an arrangement enables the culture vessels to be stacked on each other without over-compression of the foam gasket. A method of rearing juvenile (J3 stage) nematodes using such a culture vessel, which involves inoculating the contents of the culture vessel with monoxenic suspension of nematodes, is also disclosed. A method and apparatus for harvesting nematodes, a vessel for incubating nematodes for use as an inoculum, and a method of separating J3 stage and adult nematodes are also described. The culture ...

MICROORGANISM SAMPLING DEVICE

A sampling device useful for determining whether microorganisms are present on a given surface includes a lid member and a base member. The lid member includes a flange that provides visual and tactile indications to a t echnician of the position of their fingers relative to an edge of the lid member. This increases the ability to avoid accidental or inadvertent contact with a sampling media . The flange is provided with a plurality of lugs that operate to maintain some sp acing between the flange and a corresponding base member. This spacing is especial ly advantageous in situations where air flow is desirable within the sampling d evice. The base member includes a first cavity for supporting a sampling media and a second cavity. The second cavity includes a plurality of ribs that are conto ured to securely fit a lid member partially within the second cavity so that a plura lity of sampling devices can be stacked in a convenient and reliable fashion. ...

MICROPATHOLOGICAL PATIENT REPLICA BASED ON UNADULTERATED WHOLE BLOOD

A patient replica is created from a layered culture medium where solid culture medium is formed so that a discontinuity exists between the layers. An infusion port is provided in registration with the discontinuity so that a fresh unadulterated sample of patient blood can be infused into the discontinuity to form a thin layer of blood between the layers of culture medium. The thin layer obviates the requirement for any anticoagulant allowing blood-borne pathogens to be readily cultured without using broth. Further antibiotics or other drug samples may be placed on the surface of the culture medium above the blood layer so that the antibiotic can diffuse through the culture medium and reveal the sensitivities of the cultured pathogens. Other samples of pathogens or tissues can be placed on the surface of the culture medium so that effects of drugs or growth factors present in the patient blood can be observed thereby allowing the entrapped blood layer to act as a biological replica of the ...

FILTRATION ASSEMBLY

A filtration assembly for use in culturing microorganisms includes a sample reservoir for holding a fluid to be filtered, a base which can be engaged with a lower end of the sample reservoir to support a filter element during filtration, and a cover assembly for covering an upper end of the sample reservoir. The cover assembly is capable of being used as a petri dish by itself, or a portion of the cover assembly can be combined with the base to form a petri dish.

Feuchtigkeitsundurchlässiger Behälter für Nährböden

Schalenförmiger Behälter für Bakterien-Nährböden

Récipient pour l'étude de la croissance et de la physiologie des bactéries

Behälter zur mikroskopischen Untersuchung von Nährböden

Vorrichtung zur Bestimmung von Antigenen

BOX AIRTIGHT CLOSURE SEAL.

PETRI PLATE.

PROCEDURE FOR THE REGULATION OF MICROBE CONTENTS IN A DISK CASTING PROCEDURE AS WELL AS FOR UTILIZATION BOWLS.

Device for storing and handling petri dishes, comprises storage device for supporting petri dishes with lid, inspection device for automated inspection of petri dishes without lid, and transfer device for transferring petri dishes

The device comprises a storage device for supporting petri dishes with a lid, an inspection device (2) for automated inspection of the petri dishes without the lid, and a transfer device (3) for transferring the petri dishes between the storage device and the inspection device. The transfer device comprises a gripper, with which each petri dish is laterally gripped without the lid at a bottom wall. The petri dishes comprise a bottom and the lid, where a bottom wall is arranged at a circumference of the bottom. The transfer device comprises a transfer station (4). The device comprises a storage device for supporting petri dishes with a lid, an inspection device (2) for automated inspection of the petri dishes without the lid, and a transfer device (3) for transferring the petri dishes between the storage device and the inspection device. The transfer device comprises a gripper, with which each petri dish is laterally gripped without the lid at a bottom wall. The petri dishes comprise a bottom ...

СПОСОБ СКРИНИНГА МИКРОМИЦЕТОВ, КОТОРЫЕ ПРОДУЦИРУЮТ МЕЛАНИНОВЫЕ ПИГМЕНТЫ С ВЫСОКИМ УРОВНЕМ АНТИОКСИДАНТНОЙ АКТИВНОСТИ

Способ скрининга микромицетов, которые продуцируют меланиновые пигменты с высоким уровнем антиоксидантной активности, по физико-биологическим параметрам предусматривает выращивание исследуемых штаммов на агаризированной культуральной среде, определение их радиотропизма.

Extrusion type leakage-proof, height-increasable and easy-to-press handheld surface sampling culture dish

Extrusion-type quantitative easy-to-press handheld surface sampling culture dish

Culture dish for ultrasonic experiment and use method

Press-out type leakage-proof visual surface sampling petri dish easy in marking

Culture dish for blood culture

Multipurpose culture dish for supplementary reproduction experiments

The utility model relates to a cultivation equipment technical field is a multipurpose culture dish for supplementary reproduction experiments particularly, including discoid household utensils, be equipped with one deck safety wall in the discoid household utensils outer wall, be equipped with the inner wall in the safe wall, be equipped with the clearance between safe wall and the inner wall and constitute safety groove, interior pars intramuralis is equipped with a horizontal separators and a longitudinal baffle. Compared with the prior art, the utility model, its advantage lies in: this device has set up multilayer structure, is equipped with one deck safety wall in traditional culture dish, is equipped with the clearance between safe wall and the inner wall and constitutes safety groove, has guaranteed sealed effect, avoids the culture solution to spill over, the inner wall is equipped with the baffle, separates single culturing space for a plurality of regions, can accomplish a plurality ...

Anaerobic cell culture device

High-resolution imaged cell culture dish with glass bottom

Biological cell culture ware

Microorganism culture dish strutting arrangement with anticollision buffer function

Biological bacterium community culture device for wastewater treatment

Culture system

Gel seal culture dish

A device special culture dish structure

Cell culture device lives

Cracking type quantitative visible easy-to-press square counting surface sampling culture vessel

Injection device for hair follicle stem cell culture and use method thereof

Apparatus for combined incubation and vitrification of biological material

Strain culture dish for biological medicine

Culture apparatus

The utility model discloses a culture apparatus, including dish body, ware lid, support, capsule and culture membrane, dish body top surface opening, ware lid fits dish body's opening part, the support card hold in in the dish body, be equipped with the support array with the screens of capsule assorted, the capsule block in in the screens, capsule bottom surface opening just the culture membranecomplex in the opening part of capsule, the culture membrane is ion treatment's such as process culture membrane. This culture apparatus is through cultivateing multiple cell at same environment, indirect contact, interact between the cell, simulation and internal similar microenvironment to it can not provide normal and organize the problem of growing environment to solve the monolayer culture method, the environment is inconsistent in order solve to cultivate, the problem that wastes time and energy etc.

Fibroblast gene transfection method

The invention discloses a fibroblast gene transfection method, which comprises: obtaining a fibroblast suspension with a cell concentration of 1-2/uL, placing the fibroblast suspension in a cell culture dish to prepare cell suspension microdroplets, each cell suspension microdroplet being formed by the fibroblast suspension with a volume of 10 +/-0.5 uL; incubating the cell culture dish containing the cell suspension microdroplets in a set dish microenvironment, and adding the cells into a cell culture medium for culturing after the cells are adhered to the wall; the concentration of the plasmid solution is 150-200 ng/uL, and the plasmid solution contains the linearized target gene; sucking the plasmid solution into an injection needle, adjusting the included angle between the injection needle and the horizontal plane to be 30-60 degrees, and injecting the plasmid solution into each cell in sequence; and performing single cell culture after injection to obtain stably transfected transgenic ...

BOITE POUR L'OBSERVATION AU MICROSCOPE DE CULTURES CELLULAIRES VIVANTES

L'INVENTION CONCERNE UNE BOITE POUR L'OBSERVATION AU MICROSCOPE DE CULTURES CELLULAIRES VIVANTES. CETTE BOITE COMPREND AU MOINS UN PUITS 12 DONT LE FOND EST REALISE PAR UNE LAME PORTE-OBJET 14 EN VERRE POUR MICROSCOPE, ET DONT LES PAROIS SONT REALISEES EN UNE MATIERE PLASTIQUE DE QUALITE ALIMENTAIRE, LEDIT PUITS ETANT ASSEMBLE A LA LAME PORTE-OBJET PAR COLLAGE, LA HAUTEUR DE L'ENSEMBLE ETANT CHOISIE POUR PERMETTRE LE PASSAGE DE LA BOITE ENTRE LA PLATINE ET LA LENTILLE FRONTALE D'UN CONDENSEUR DE MICROSCOPE.

Boîte de culture

Boîte de culture comprenant un réceptacle 1 qui est recouvert d'un couvercle 2 et sur le fond duquel est déposée une couche d'un milieu de culture. Deux orifices 3, 4, ménagés sur les faces en regard du réceptacle 1 et du couvercle 2, permettent de mettre l'intérieur du réceptacle en communication avec l'extérieur, alors même que le réceptacle 1 est recouvert du couvercle 2. Analyses médicales.

LIMP STERILE FOR THE CULTURE OF MICRO-ORGANISMS, TOGETHER AND DISTRIBUTOR UNCOUPLE

DEVICE ALLOWING TO OBTAIN an ATMOSPHERE ENRICHED IN CARBONIC GAS AND/OR Free from OXYGEN, IN a TIGHT CONTAINER

Dispositif pour remplir des boîtes de pétri

Ce dispositif est équipé d'un plateau de transfert tournant 1 apte à acheminer les boîtes à l'état ouvert d'une station distributrice de boîtes vides à une station de remplissage, puis de cette dernière à une station réceptrice de boîtes remplies; ce plateau comprend une platine supérieure 10 dont les ouvertures retiennent les couvercles des boîtes et une platine inférieure 11 dont les ouvertures sont traversées par les fonds de boîtes, ces fonds se trouvant constamment en appui sur une semelle fixe réfrigérée 55 au cours de leur transfert; l'entraînement du plateau tournant 1 se fait au moyen d'un disque moteur 6 placé sous la semelle 55, par l'intermédiaire d'un accouplement magnétique comprenant une série d'aimants 71-12 placés en vis-à-vis. Equipement de laboratoire.

SAFETY DEVICES FOR the TAKING AWAY OF MICRO-ORGANISMS IN the AIR

Boîte perfectionnée pour milieux de culture utilisables en bactériologie

Cette boîte consiste en un réceptacle unique 1 destiné à recevoir simultanément au moins deux milieux de culture différents grâce à une séparation entre eux réalisée par au moins une cloison 2, 3.

CONTAINER FOR RECEIVING ONE OR MORE CULTURE MEDIA FOR MICROORGANISMS.

LIMP OF COMPRISING $PETRI OF the MEANS OF LOCKING FOR the CONSTITUTION Of a PILE

DEVICE FOR THE ANALYSIS OF THE MICROFLORA OF THE WOOD A BARREL

PROCEEDED OF DETERMINATION OF THE CONCENTRATIONS OF MICROBES BY MEANS OF A PROCESS OF DEPOSIT IN PLATE AND UTILISEE IN THIS PROCESS LIMPS