08-08-2013 дата публикации
Номер: US20130203124A1
Принадлежит:
NOVOZYMES A/S
The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase. 1. A variant of a parent Termamyl-like alpha-amylase , comprising an alteration at one or more positions selected from the group of:5, 6, 36, 37, 38, 39, 42, 45, 47, 63, 66, 69, 70, 71, 72, 74, 75, 76, 79, 82, 83, 86, 87, 89, 93, 112, 113, 117, 120, 137, 213, 216, 220, 223, 225, 226, 227, 229, 243, 245, 279, 282, 311, 321, 324, 352, 353, 354, 357, 361, 362, 364, 368, 390, 395, 397, 399, 400, 401, 425, 451, 452, 453, 466, 468, 470, 471, 478,wherein (i) an insertion of an amino acid downstream of the amino acid which occupies the position,', '(ii) a deletion of the amino acid which occupies the position, or', '(iii) a substitution of the amino acid which occupies the position with a different amino acid,, '(a) the alteration(s) are independently'}{'i': 'Bacillus licheniformis', '(b) the variant has alpha-amylase activity and (c) each position corresponds to a position of the amino acid sequence of the parent Termamyl-like alpha-amylase having the amino acid sequence shown in SEQ ID NO: 8 (alpha-amylase).'}2. A variant of a parent Termamyl-like alpha-amylase , comprising one or more of the following substitutions:X1A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y;X2R,N,D,C,Q,E,G,H,I,L,K,M,F,S,T,W,Y,V;X3A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y;X4A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;X13R,N,D,C,Q,E,G,H,K,M,P,S,T,W;X14A,R,D,C,G,K,M,P,W;X16R,N,D,C,Q,E,G,H,I,L,K,M,F,S,T,W,Y,V;X17A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;X18A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;X20A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;X23A,R,N,D,C,Q,E,G,H,I,L,M,F,P,S,W,Y,V;X24A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;X26A,D,C,E,G,H,I,L,M,F,P,S,T,W,V;X34A,R,N,C,Q,E,G,H,I,L,K,M,F,P,T,W,Y,V;X35A,R,N,D,C,Q,E,G,H,K,M,F,P,S,T,W,Y,V;X49A,C,G,H,P,T;X50A,R,N,C,Q,E,G,H,K,M,F,P,S,W;X51A,N,D,C,Q,E,G,H,I,L,M,F,P,S,T,W,Y,V;X52A,R,D,C, ...
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