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Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 14037. Отображено 100.
20-11-2016 дата публикации

Анализатор качества дрожжей хлебопекарных

Номер: RU0000166347U1
Автор: Виталий Владимирович Баранов, Владимир Викторович Баранов, Сергей Александрович Романчиков
Принадлежит: Федеральное государственное казенное военное образовательное учреждение высшего образования "Военная академия материально-технического обеспечения имени генерала армии А.В. Хрулёва" Министерства обороны Российской Федерации

Анализатор качества дрожжей хлебопекарных, содержащий емкость, отличающийся тем, что дополнительно содержит корпус, на внешней стороне которого проделано отверстие, внутри которого фиксируется емкость, герметично закрывающаяся крышкой с герметизированным отверстием, через которое проходит провод, соединенный одним концом с комбинированным датчиком для измерения давления и температуры, а вторым концом с микроконтроллером, соединенным через провод со звуковым блоком, неподвижно зафиксированным внутри корпуса, и с дисплеем, жестко зафиксированным на внешней стороне корпуса, а также микроконтроллер соединен через кабель с ЭВМ или разъемом питания, а через провод - с преобразователем напряжения, аккумуляторной батареей и блоком питания, неподвижно закрепленными внутри корпуса. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 166 347 U1 (51) МПК G01N 33/10 (2006.01) C12Q 1/04 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ТИТУЛЬНЫЙ (21)(22) Заявка: ЛИСТ ОПИСАНИЯ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ 2015152772/15, 08.12.2015 (24) Дата начала отсчета срока действия патента: 08.12.2015 (45) Опубликовано: 20.11.2016 Бюл. № 32 Адрес для переписки: 199034, Санкт-Петербург, наб. Адмирала Макарова, 8, ООНР 1 6 6 3 4 7 R U (57) Формула полезной модели Анализатор качества дрожжей хлебопекарных, содержащий емкость, отличающийся тем, что дополнительно содержит корпус, на внешней стороне которого проделано отверстие, внутри которого фиксируется емкость, герметично закрывающаяся крышкой с герметизированным отверстием, через которое проходит провод, соединенный одним концом с комбинированным датчиком для измерения давления и температуры, а вторым концом с микроконтроллером, соединенным через провод со звуковым блоком, неподвижно зафиксированным внутри корпуса, и с дисплеем, жестко зафиксированным на внешней стороне корпуса, а также микроконтроллер соединен через кабель с ЭВМ или разъемом питания, а через провод - с преобразователем напряжения, аккумуляторной батареей и блоком питания, ...

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Номер записи: 1
22-01-2020 дата публикации

Устройство для моделирования in vivo бактериальных биопленок

Номер: RU0000195313U1
Автор: Владимир Алексеевич Гущин, Владимир Семенович Рыбкин, Евгений Олегович Рубальский, Илона Мурмановна Аршба, Марина Александровна Самотруева, Олег Васильевич Рубальский, Радмила Охасовна Абдрахманова, Сергей Владимирович Орлов
Принадлежит: Владимир Алексеевич Гущин, Владимир Семенович Рыбкин, Евгений Олегович Рубальский, Илона Мурмановна Аршба, Марина Александровна Самотруева, Олег Васильевич Рубальский, Радмила Охасовна Абдрахманова, Сергей Владимирович Орлов

Полезная модель относится к области медицины и биотехнологии, в частности к микробиологии, и предназначена для моделирования условий развития бактериальных биопленок в инфицированной полости и исследования действия антибактериальных средств, в том числе бактериофагов, кодируемых ими ферментов и их аналогов на бактериальные биопленки. Устройство для моделирования in vivo бактериальных биопленок в инфицированной полости выполнено в виде непроницаемой для бактерий замкнутой емкости с внутренней полостью объемом 100-100000 мм, стенки которой состоят частично из каркаса, выполненного из биологически совместимого материала, предпочтительно из биологически совместимого полимера, с, по крайней мере, одним выступом внутри замкнутой емкости, а частично из биологически совместимой пористой мембраны с эквивалентным диаметром пор не более 450 нм, занимающей 1-99% площади наружной поверхности устройства. Устройство может быть выполнено в произвольной форме, предпочтительно в форме шара или усеченного шара, или сфероида, или усеченного сфероида, или цилиндра, или конуса, или усеченного конуса, или икосаэдра, или усеченного икосаэдра. Техническим результатом является создание устройства, позволяющего моделировать in vivo бактериальные биопленки в инкапсулированном, ограниченном инфекционном очаге в виде инфицированной полости без опасности генерализации инфекции. 1 з.п. ф-лы, 1 ил., 3 пр. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 195 313 U1 (51) МПК C12M 1/14 (2006.01) C12M 1/34 (2006.01) C12Q 1/02 (2006.01) C12Q 1/04 (2006.01) C12N 1/20 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ (52) СПК C12M 1/14 (2019.08); C12M 1/34 (2019.08); C12Q 1/04 (2019.08); C12Q 1/02 (2019.08); C12N 1/20 (2019.08) (21)(22) Заявка: 2019128205, 09.09.2019 (24) Дата начала отсчета срока действия патента: Дата регистрации: 22.01.2020 (45) Опубликовано: 22.01.2020 Бюл. № 3 1 9 5 3 1 3 R U (56) Список документов, цитированных в отчете о поиске: COENYE T. ...

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Номер записи: 2
01-03-2012 дата публикации

OLIGONUCLEOTIDES FOR DETECTING E. coli O157:H7 STRAINS AND USE THEREOF

Номер: US20120052494A1
Автор: Jason Opdyke, Jun Li, Win Den Cheung
Принадлежит: Samsung Techwin Co Ltd

Oligonucleotides, a kit, and a method for detecting E. coli O157:H7 strains are provided. According to the kit for detecting E. coli O157:H7 strains and the method of detecting E. coli O157:H7 strains by using the kit, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time.

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Номер записи: 3
10-05-2012 дата публикации

Container for testing for micro-organisms

Номер: US20120115216A1
Автор: Rosemary Katherine Cameron Sharpin
Принадлежит: ZYZEBA TESTING Ltd

A multi-compartment resealable container for testing for the presence of micro-organisms is provided with or adapted to receive a sample in a first substantially rigid transparent compartment, a growth medium in a second compartment, and a sanitizer in a third compartment, the compartments being separated by foil seals, the second and third compartments have an associated plunger which when depressed causes serrated cutters to puncture the foil seal and allow the contents to be added to the liquid containing or comprising the sample.

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Номер записи: 4
17-05-2012 дата публикации

Real-time method for the detection of viable micro-organisms

Номер: US20120122147A1
Автор: Frank Schuren, Remco Kort, Roy Montun
Принадлежит: Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO

The invention relates to a method for real-time detection of viable microorganisms comprising: a. addition of a cell-permeable, phototautomeric compound to a micro-organism or other living cell; and b. measuring the fluorescent emission of said phototautomeric compound. Preferably the phototautomeric compound is salicylic acid, 2-hydroxy-1-naphtoic acid or 1-hydroxy-2-naphtoic acid. Further, the assay can he used to assess the antibiotic effect of a test compound. This test can be used as a high—throughput screening for compounds with antibiotic activity. Also part of the invention is the use of a cell permeable phototautomeric compound in a method for determining the viability of micro-organisms and for assessing the antibiotic effect of a test compound.

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Номер записи: 5
07-06-2012 дата публикации

Water cooler towers and other man-made aquatic systems as environmental collection systems for agents of concern

Номер: US20120137756A1
Автор: Mark T. Kingsley, Robin Brigmon
Принадлежит: SAVANNAH RIVER NUCLEAR SOLUTIONS LLC

An apparatus and process of using existing process water sources such as cooling towers, fountains, and waterfalls is provided in which the water sources are utilized as monitoring system for the detection of environmental agents which may be present in the environment. The process water is associated with structures and have an inherent filtering or absorbing capability available in the materials and therefore can be used as a rapid screening tool for quality and quantitative assessment of environmental agents.

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Номер записи: 6
28-06-2012 дата публикации

Method of classifying microorganisms using UV irradiation and excitation fluorescence

Номер: US20120161035A1
Автор: Burt V. Bronk
Принадлежит: Individual

A method and device for detecting, differentiating from background and providing partial identification (i.e., classification) for biological particles found in aerosols or surface dust. The method is based on the phenomenon that luminescent excitation-emission (EEM) graphs of microorganisms obtained before and after perturbation by irradiation with ultraviolet light show characteristic patterns which differ according to the type of particle. For example, Bacillus endospores may be distinguished from vegetative bacteria, and gram positive vegetative bacteria may be distinguished from gram negative bacteria, and all these may be distinguished from many types of background particles, e.g. house dust, road dust, and pollen.

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Номер записи: 7
28-06-2012 дата публикации

Apparatus and method for analyzing bacteria

Номер: US20120166093A1
Автор: Yasuyuki Kawashima
Принадлежит: Sysmex Corp

An apparatus for analyzing bacteria is described that includes an analytic sample preparation section for preparing an analytic sample by treating a specimen so as to generate a morphological difference between Gram-negative bacteria and Gram-positive bacteria, a detector for detecting optical information from each particle contained in the analytic sample and an analyzing section for detecting Gram-positive bacteria contained on the basis of the detected optical information. A method for analyzing bacteria is also described.

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Номер записи: 8
19-07-2012 дата публикации

Peristaltic pumps and filtration assembly systems for use therewith

Номер: US20120180577A1
Автор: Michael Scott Mcclain, Thomas J. Bormann, William Charles Steere
Принадлежит: Pall Corp

Filtration assemblies for use with peristaltic pumps, peristaltic pumps, systems including the assemblies and pumps, and methods of using the assemblies, pumps, and systems, are disclosed.

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Номер записи: 9
19-07-2012 дата публикации

Method for testing drug sensitivity of mycobacterium tuberculosis, application of indicator, and solid medium

Номер: US20120183991A1
Автор: Jun Peng
Принадлежит: Individual

A method for testing of drug sensitivity of Mycobacterium tuberculosis. The method includes: pre-treating a specimen, adding a liquid medium to conduct enrichment culturing, heating a solid medium, mixing the specimen after enrichment culturing with the solid medium in a liquid state, cooling, and preparing a solid specimen for testing of drug sensitivity with drug sensitivity paper, culturing for 5-15 days, and observing an inhibition zone through the indication of an indicator.

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Номер записи: 10
02-08-2012 дата публикации

Method and assembly for determining cell vitalities

Номер: US20120196318A1
Автор: Harald Schmidt, Manfred Stanzel, Markus Klöpzig, Peter Paulicka, Walter Gumbrecht
Принадлежит: SIEMENS AG

The method includes binding living cells to magnetic particles, adding them to a sensor array, uniformly distributing over the sensor array, magnetically fixing the magnetic particles having the bound cells over the sensor array, and adding substances to maintain and/or improve the cell vitality to the sensory array, and/or adding substances to worsen the cell vitality to the sensor array. The assembly includes a sensor array composed of sensors, which are designed to be in direct fluidic contact with a fluid, and a device for generating a magnetic field over the sensor array. A layer that comprises magnetic particles and living cells is formed on the sensor array.

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Номер записи: 11
27-09-2012 дата публикации

Method for detecting microorganisms belonging to mycoplasma pneumoniae and/or mycoplasma genitalium

Номер: US20120244544A1
Автор: Atsuko Minagawa, Hatsue Itagaki, Kazuyuki Sugiyama, Toyomasa Hiroshima, Yasushi Shimada, Yuki Mitobe
Принадлежит: LSI Medience Corp

A detection method and a detection kit for rapidly and specifically diagnosing Mycoplasma pneumoniae and/or Mycoplasma genitalium infections are provided. The DnaK of Mycoplasma pneumoniae or Mycoplasma genitalium is used as an indicator.

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Номер записи: 12
01-11-2012 дата публикации

Kit for detection of microorganism

Номер: US20120277121A1
Автор: Shinichi Yoshida, Takashi Soejima
Принадлежит: Morinaga Milk Industry Co Ltd

A kit for use in a method for detecting live cells, injured cells and dead cells of a microorganism in a test sample by a nucleic acid amplification method is disclosed. The kit includes a cross-linker capable of cross-linking DNA by irradiation with light having a wavelength of 350 nm to 700 nm, medium, and a primer (s) for amplifying a DNA target region in the microorganism by a nucleic acid amplification method. The nucleic acid amplification method may be a PCR, LAMP, SDA, LCR or DNA microarray method. A cross-linker may be included in the kit such as ethidium monoazide, ethidium diazide, psoralen, 4,5′,8-trimethyl psoralen, or 8-methoxy psoralen.

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Номер записи: 13
29-11-2012 дата публикации

Rapid Detection of Molds that Produce Glucose Oxidase

Номер: US20120301911A1
Автор: Michael J. Svarovsky, Stephanie J. Moeller, Stephen B. Roscoe
Принадлежит: Individual

Methods and kits are disclosed for detecting microorganisms that produce glucose oxidase. The method includes providing a culture medium and a hydrogen peroxide indicating reagent comprising a chromogenic substrate that can provide a detectable chromogenic reaction indicating the presence of a microorganism that produces glucose oxidase, and additional methods are disclosed for differentiating microorganisms by the detection of an additional chromogenic reaction.

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Номер записи: 14
27-12-2012 дата публикации

Testing of Biofilm for Anti-microbial Agent Susceptibility

Номер: US20120329675A1
Автор: Howard Ceri, Merle E. Olson
Принадлежит: Individual

This invention is an apparatus and method for susceptibility testing one or more biofilms, for selecting one or more anti-microbial combinations with efficacy against the biofilm, and/or in treating a disease or condition mediated by the biofilm The invention includes methods for the selection of antibiotic combinations with efficacy against a specific microbial type and for the formulation of microbe-specific test plates. The invention also includes an assay system to test patient specific isolates for sensitivity to the anti-microbial combinations.

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Номер записи: 15
24-01-2013 дата публикации

Use of Ribozymes in the Detection of Adventitious Agents

Номер: US20130022964A1
Автор: Matthew C. Coffey
Принадлежит: Oncolytics Biotech Inc

The present invention provides a method of detecting adventitious agents in a composition comprising a microorganism by using ribozyme-expressing indicator cells, as well as indicator cells useful in such detection.

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Номер записи: 16
24-01-2013 дата публикации

Method for the non-specific enrichment of microorganisms

Номер: US20130022984A1
Автор: Frank Narz
Принадлежит: QIAGEN GmbH

The present invention relates to a method for the non-specific enrichment of microorganisms from complex starting materials, wherein the starting materials containing the microorganisms are brought in contact with cells of the innate immune system, the microorganisms are bound to the cells of the innate immune system and the binding complex is separated from the complex starting material.

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Номер записи: 17
28-02-2013 дата публикации

Apparatus and method for evaluation of antimicrobial air filter efficiency

Номер: US20130052680A1
Автор: Gi Byoung HWANG, Gwi Nam Bae, Jae Hee Jung, Seung Bok Lee, Seung Jae Lee
Принадлежит: Korea Advanced Institute of Science and Technology KAIST

Disclosed are an apparatus and a method for evaluating the efficiency of an antimicrobial air filter, which allow precise determination of the efficiency of an antimicrobial air filter by dividing microorganisms deposited on the antimicrobial air filter into living microorganisms and dead microorganisms by use of two types of fluorescent dyes, and by analyzing the microorganisms quantitatively.

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Номер записи: 18
14-03-2013 дата публикации

Culture medium, method for culturing salmonella and e. coli and method for detecting salmonella and e. coli

Номер: US20130065240A1
Автор: Gabriela Martinez, Janikim LAROCHELLE, Renaud TREMBLAY
Принадлежит: Foodchek Systems Inc

In embodiments there are disclosed culture media, compositions for supplementing culture media, and methods for culturing biological samples. In embodiments the methods are methods for detecting bacteria and in embodiments the bacteria include Salmonella spp and in embodiments include E. coli spp. In embodiments the media and compositions comprise an alkanolamine and a cobalamin compound.

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Номер записи: 19
28-03-2013 дата публикации

Method And Devices For The Cross-Referencing Of Identification Of Tissue Slice Supports For Microtomised Analytical Samples

Номер: US20130078669A1
Автор: Hans L. Heid, Jose Novoa
Принадлежит: MICROM INTERNATIONAL GMBH

The invention relates to a method and device for the cross-referencing of identification ( 1 ) of tissue slice supports ( 2 ), for microtomised analytical samples still to be mounted thereon, with identification information ( 3 ) of a tissue sample holder ( 4 ) of a tissue sample ( 5 ) which is not yet microtomised. The conventional problem of cross-referencing is improved in a simple manner, whereby the identification information ( 3 ) for the tissue sample holder ( 4 ) is automatically detected when positioned in the microtome ( 6 ) and an identification ( 1 ) corresponding thereto is automatically transferred to at least one tissue slice support ( 2 ) and that tissue slice support ( 2 ), provided with the identification ( 1 ), is dispensed for application of the tissue sample slice at the moment when a tissue sample slice must be applied to a tissue slice support ( 2 ).

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Номер записи: 20
04-04-2013 дата публикации

Rapid and sensitive detection of bacteria in blood products, urine, and other fluids

Номер: US20130084588A1
Автор: Daniel G. Ericson, Kyle R. Brandy
Принадлежит: Zybac LLC

The invention provides methods of detecting bacteria in fluids, including blood, platelets and other blood products for transfusion, and urine. The methods are based on lysing the bacteria to release ATP and detecting the ATP. Eukaryotic cell contamination is a problem to be overcome, because eukaryotic cell contain large amounts of ATP. Thus, some of the methods involve separating intact eukaryotic cells (e.g., platelets) from intact bacterial cells before lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme that catalyzes a reaction, and monitoring the enzyme-catalyzed reaction. Typically, the enzyme is luciferin, and the reaction is monitored by detecting light produced by the luciferin. Other methods of the invention involve contacting a fluid sample with a support surface that binds bacterial cells, lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme, and monitoring the enzyme-catalyzed reaction. Apparatuses for carrying out the methods are also disclosed.

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Номер записи: 21
04-04-2013 дата публикации

Method for Detecting Clusters of Biological Particles

Номер: US20130084598A1
Автор: Bossy Geneviève, Drazek Laurent, Dupoy Mathieu, Figadere Olivier, Guicherd Maryse, Mallard Frédéric, Moy Jean-Pierre, Pinston Frédéric, Romanens Fabien
Принадлежит:

The invention relates to a method for detecting clusters of biological particles () on a surface (), comprising steps that involve: a. determining (E) a topographical representation () of said surface; and b. detecting (E E), on said topographical representation, at least one contour defining a region that is likely to correspond to a cluster of biological particles. 1. A method of detecting a cluster of biological particles on a surface , the method comprising the steps:a) determining a topographical representation of said surface; andb) detecting in said topographical representation at least one contour defining a region that potentially corresponds to a cluster of biological particles;these steps being implemented with the help of electronic data processor means.2. A method according to claim 1 , wherein said biological particles are selected from microorganisms claim 1 , vegetable claim 1 , and animal cells.3. A method according to claim 1 , wherein said biological particles present a diameter or a main dimension that is less than or equal to 100 μm.4. A method according to claim 1 , wherein said surface is selected from the group consisting of the interface between a culture medium and a surrounding medium claim 1 , the surface of a functionalized substrate claim 1 , and the surface of a microporous membrane.5. A method according to claim 1 , wherein said step a) consisting in determining a topographical representation of said surface is implemented by an optical method performed without contact and without preparing the sample.6. A method according to claim 5 , wherein said step a) consisting in determining a topographical representation of said surface is implemented by chromatic confocal microtopography.7. A method according to claim 5 , wherein said step a) consisting in determining a topographical representation of said structure is implemented by Schlieren photography or by ombroscopy.8. A method according to claim 1 , wherein said step b) consists in ...

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Номер записи: 22
11-04-2013 дата публикации

Compositions, methods and kits to detect adenovirus nucleic acids

Номер: US20130089859A1
Автор: Emily Ziegler, Jessica Townsend
Принадлежит: Gen Probe Prodesse Inc

The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Adenovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers.

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Номер записи: 23
11-04-2013 дата публикации

APPARATUS AND METHOD FOR ANALYZING A CONTAMINATED SURFACE

Номер: US20130089890A1
Автор: Pflanz Karl
Принадлежит: SARTORIUS STEDIM BIOTECH GMBH

An apparatus () for analyzing a contaminated surface has a transfer device () with a mount () and a porous disk-shaped medium () having a contact side () arrangeable on the contaminated surface. The mount () is bindable to the medium on its side () facing away from the contact side () via a first fixing edge (). An analytical device () is bindable to the medium () on its contact side () via a second fixing edge () to remove the medium () from the mount (). The first fixing edge () is bindable to the medium () via a first adhesive bond and the second fixing edge () is bindable to the medium () via a second adhesive bond. The first adhesive bond is breakable by a lower application of force than the second adhesive bond. 11. An apparatus () for analyzing a contaminated surface , comprising{'b': 2', '3', '5', '6', '8', '3', '6', '5', '7, 'i': 'a', 'a transfer device () having a mount () and a porous disk-shaped medium () having a contact side () arrangeable on the contaminated surface, a side () of the mount () facing away from the contact side () being bindable to the medium () via a first fixing edge (), and'}{'b': 11', '5', '6', '7', '5', '3, 'i': 'b', 'an analytical device () that is bindable to the medium () on its contact side () via a second fixing edge () in order to remove the medium () from the mount (),'}{'b': 7', '5', '7', '5, 'i': a', 'b, 'wherein the first fixing edge () is bindable to the medium () via a first adhesive bond and the second fixing edge () is bindable to the medium () via a second adhesive bond and wherein the first adhesive bond is breakable by a lower application of force than the second adhesive bond.'}214351156754b. The apparatus () of claim 1 , wherein a supporting layer () is arranged between the mount () and the medium () and wherein the analytical device () is bindable to the medium () on its contact side () via the second fixing edge () in order to remove the medium () from the supporting layer ().31. The apparatus () of claim 2 , ...

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Номер записи: 24
11-04-2013 дата публикации

APPARATUS FOR CHEMILUMINESCENT ASSAY AND DETECTION

Номер: US20130089891A1
Автор: NODA Hideyuki, OKANOJO Masahiro, Ozawa Satoshi, UCHIDA Kenko
Принадлежит: Hitachi, Ltd.

An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less. 1. A luminescence assay and detection apparatus , comprising:a holder for holding a container storing a sample;a photodetector for detecting light from the container; a light-shielding plate provided between the photodetector and the holder;a light-shielding plate driving section for moving the light-shielding plate; anda photodetector position control section for moving the photodetector,wherein the light-shielding plate driving section moves the light-shielding plate from a position facing the photodetector after the container is set,wherein the photodetector position control section positions a light receiving surface of the photodetector in such a manner as to face the container after the light-shielding plate is moved, and after the light receiving surface of the photodetector is positioned, controls a distance between a bottom portion of the container and the photodetector facing the container in accordance with a signal intensity of the light when the photodetector performs luminescence assay and detection of the sample.2. The luminescence assay and detection apparatus according to claim 1 , wherein the photodetector position ...

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Номер записи: 25
18-04-2013 дата публикации

Mass spectrometric measurement of b-lactamase resistances

Номер: US20130095511A1
Автор: Karsten Michelmann, Katrin Sparbier, Markus Kostrzewa
Принадлежит: Individual

The invention relates to the determination of resistances of microorganisms which produce β-lactamases, in particular “extended spectrum β-lactamases” (ESBL). The invention provides a method whereby the microbial resistance can be measured very simply and quickly by means of the catalytic effect of the microbially produced β-lactamases on β-lactam antibiotics, which consists in a hydrolytic cleavage of the β-lactam ring. The method determines the resistance of the bacteria a few hours after a suitable substrate, either a β-lactam antibiotic or a customized β-lactam derivative, has been added to a suspension of the microbes, by direct mass spectrometric measurement of the substrate breakdown caused by the β-lactamases.

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Номер записи: 26
25-04-2013 дата публикации

METHOD OF USING NEAR INFRARED FLUORESCENT DYES FOR IMAGING AND TARGETING CANCERS

Номер: US20130101513A1
Автор: Chung Leland W.K., Wang Ruoxiang, Yang Xiaojian, Zhau Haiyen E.
Принадлежит:

The present invention describes methods of identifying, detecting, imaging, isolating and locating cancer cells in a subject. The method invokes the use of near-infrared (NIR) organic carbocyanine dyes, particularly, near infrared heptamethine cyanine dyes and the detection of the fluorescence of these NIR dyes. The uptake of these dyes by cancer cells and not by normal cells, as well as their high intensity, among other things, allow for the detection of cancerous cells in a subject and facilitate their subsequent isolation. Further, detection of many tumor types and tumor cell populations under cell culture and in vivo conditions are described. 1. A method , comprising:providing a biological sample from a subject;contacting the biological sample with a composition comprising a near-infrared (NIR) organic carbocyanine dye to form a mixture; andanalyzing the mixture to identify, detect, locate, isolate and/or characterize a possible cancer cell or tumor in the biological sample.2. The method of claim 1 , wherein the biological sample is selected from the group consisting of tissue claim 1 , tumor tissue claim 1 , cancer tissue claim 1 , cell claim 1 , tumor cell claim 1 , cancer cell claim 1 , body fluid claim 1 , whole blood claim 1 , plasma claim 1 , stool claim 1 , intestinal fluid or aspirate claim 1 , stomach fluid or aspirate claim 1 , serum claim 1 , cerebral spinal fluid (CSF) claim 1 , urine claim 1 , sweat claim 1 , saliva claim 1 , tears claim 1 , pulmonary secretion claim 1 , breast aspirate claim 1 , breast milk claim 1 , prostate fluid claim 1 , seminal fluid claim 1 , cervical scraping claim 1 , bone marrow aspirate claim 1 , amniotic fluid claim 1 , intraocular fluid claim 1 , mucous claim 1 , moisture in breath.3. The method of claim 1 , wherein the method identifies or detects the presence of a cancer cell or a tumor in the biological sample when a presence of an increased NIR fluorescent signal claim 1 , relative to a background staining intensity ...

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Номер записи: 27
25-04-2013 дата публикации

OPTICAL FLUORESCENCE DUAL SENSORS AND METHODS OF PREPARING AND USING THEM

Номер: US20130102024A1
Автор: Holl Mark, Jin Yuguang, Lu Hongguang, Meldrum Deirdre, Tian Yanqing, ZHANG Weiwen
Принадлежит:

The present invention relates to an optical fluorescence dual sensor comprising a probe for sensing pH, a probe for sensing oxygen, an intra-reference probe and a matrix. The present invention also relates to methods of preparing an optical fluorescence dual sensor and methods of using them. 9. A method of determining pH of a sample comprising{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(a) exposing the sample to an optical fluorescence dual sensor according to ;'}(b) irradiating the sensor at a first wavelength to produce a pH indicator emission signal at a second wavelength and an intra-reference emission signal at a third wavelength;(c) measuring the pH indicator emission signal at the second wavelength;(d) measuring the intra-reference emission signal at the third emission wavelength; and(e) ratiometrically determining the pH of the sample.10. A method of determining oxygen concentration in a sample comprising{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(a) exposing the sample to an optical fluorescence dual sensor according to ;'}(b) irradiating the sensor at a first wavelength to produce an oxygen indicator emission signal at a second wavelength and an intra-reference emission signal at a third wavelength;(c) measuring the oxygen indicator emission signal at the second wavelength;(d) measuring the intra-reference emission signal at the third wavelength; and(e) ratiometrically determining the oxygen concentration in the sample.11. A method of simultaneously determining pH and oxygen concentration in a sample{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(a) exposing the sample to an optical fluorescence dual sensor according to ;'}(b) irradiating the sensor at a first wavelength to produce a pH indicator emission signal at a second wavelength, an oxygen indicator emission signal at a third wavelength and an intra-reference emission signal at a fourth wavelength;(c) measuring the pH indicator emission signal at the second wavelength;(d) measuring ...

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Номер записи: 28
25-04-2013 дата публикации

METHODS AND COMPOSITIONS FOR IDENTIFYING A CELL PHENOTYPE

Номер: US20130102025A1
Автор: CHAUSOVSKY Alexander, DAVIS Noa, Elkeles Adi, Terkieltaub Dov
Принадлежит: ZETIQ TECHNOLOGIES LTD.

The present invention is directed to a method of identifying cancerous or precancerous cells suspended in a liquid carrier, such as, a biological fluid. 1. A method of identifying at least one precancerous or cancerous cell present in a cell suspension , the method comprising:(a) providing a sample comprising a plurality of cells suspended in a liquid carrier;(b) fixing the plurality of cells onto a solid substrate;(c) contacting said plurality of cells with a Ficus plant extract;(d) contacting said plurality of cells with at least one dye; and(e) analyzing said plurality of cells,wherein when: (i) said at least one dye is an acidic dye, a staining intensity of at least one cell above a predetermined threshold is indicative that said at least one cell is a differentiated cell; (ii) said at least one dye is a basic dye, a staining intensity of at least one cell above a predetermined threshold is indicative that said at least one cell is an undifferentiated cell; (iii) said at least one dye comprises said acidic dye and said basic dye, a staining intensity of at least one cell above a predetermined threshold with said acidic dye is indicative that said at least one cell is a differentiated cell and a staining intensity of at least one cell above a predetermined threshold with said basic dye is indicative that said at least one cell is an undifferentiated cell.2. The method of claim 1 , wherein said at least one dye is a basic dye or an acidic dye.3. The method of claim 2 , wherein said basic dye is selected from the group consisting of Dahlia and new Fuchsin.4. The method of claim 2 , wherein said acidic dye is selected from the group consisting of Fast Green and Light Green.5. The method of claim 1 , wherein said at least one dye is a single dye or comprises two dyes.6. The method of claim 1 , wherein said Ficus plant is Ficus elastica.7. The method of claim 1 , wherein said Ficus plant extract is an ethanol extract of a leaf tissue.8. The method of claim 1 , wherein ...

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Номер записи: 29
25-04-2013 дата публикации

METHOD FOR THE DIAGNOSIS OF TUBERCULOSIS

Номер: US20130102491A1
Автор: Oesch Bruno, Schiller Irene, Vordermeier Martin
Принадлежит: PRIONICS AG

A method for the diagnosis of a tuberculosis infection caused by Mycobacteria belonging to the Mycobacteria tuberculosis complex group (MTC) in an animal including a human being, which comprises in vitro-detection of cell-mediated immune response to OmpAtb and/or antibodies against OmpAtb in a sample taken from that animal. 1. A method for diagnosing a tuberculosis infection in an animal caused by Mycobacteria belonging to the Mycobacteria tuberculosis complex group , the method comprising:acquiring sample material or tissue from the animal from which the detection of antibodies or a cell mediated immune response upon contact with OmpAtb can be detected upon analysis;contacting the sample material or tissue acquired from the animal with a test reagent comprising an antigen having antigenicity of OmpAtb; anddetecting in vitro the presence or absence of antibodies to OmpAtb or a cell mediated immune response to OmpAtb in the sample material or tissue contacted with the test reagent;wherein the presence of antibodies to OmpAtb or a cell-mediated immune response to OmpAtb in the sample material or tissue contacted with the test reagent constitutes a positive diagnosis of a tuberculosis infection in the animal from which the sample material or tissue was acquired caused by Mycobacteria belonging to the Mycobacteria tuberculosis complex group, andwherein the absence of antibodies to OmpAtb or a cell-mediated immune response to OmpAtb in the sample material or tissue contacted with the test reagent constitutes a negative diagnosis of a tuberculosis infection in the animal from which the sample material or tissue was acquired caused by Mycobacteria belonging to the Mycobacteria tuberculosis complex group.2. The method according to claim 1 , wherein the antigen having antigenicity of OmpAtb is OmpAtb.3. The method according to claim 2 , wherein the test reagent further comprises at least one further mycobacterial antigen.4. The method according to claim 3 , wherein the at ...

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Номер записи: 30
02-05-2013 дата публикации

METHOD FOR DIAGNOSING RISK OF TYPE 1 DIABETES AND FOR PREVENTING ONSET OF TYPE 1 DIABETES

Номер: US20130108598A1
Автор: Maukonen Johanna, Oresic Matej, Saarela Maria, Sysi-Aho Marko
Принадлежит: TEKNOLOGIAN TUTKIMUSKESKUS VTT

The invention comprises methods for diagnosing the risk of onset of type 1 diabetes and for preventing the onset of type 1 diabetes. The genetic background, metabolomes, antibodies and diversity of gut microbiota of an individual can be used for diagnosis. 4. A method of wherein the metabolite in case [A] is lysophosphatidylcholine.5. A method of wherein genetic background of an individual is estimated.6Clostridium leptum. Method for preventing onset of type 1 diabetes in an individual by administering to said individual at least one species of bacteria of the clostridial phylogenetic cluster IV (group) or a metabolite thereof.7. Method of wherein said individual has an increased risk of type 1 diabetes or is susceptible for developing type 1 diabetes.8Clostridium leptum. Method of wherein the susceptibility or increased risk is measured by diagnosing a diminished diversity of the group bacteria.9. Method of wherein the bacteria to be administered is non-pathogenic. This invention relates to a novel method for diagnosing an increased risk of type 1 diabetes in an individual. Furthermore, this invention relates to a method for preventing onset of type 1 diabetes in an individual.The publications and other materials used herein to illuminate the background of the invention, and in particular, cases to provide additional details respecting the practice, are incorporated by reference.Type 1 diabetes (T1D) is an autoimmune disease that results from the selective destruction of insulin-producing β-cells in pancreatic islets. The diagnosis of T1D is commonly preceded by a long prodromal period which includes seroconversion to islet autoantibody positivityand subtle metabolic disturbances. The incidence of T1D among children and adolescents has increased markedly in the Western countries during the recent decadesand is presently increasing at a faster rate than ever before. This suggests an important role of environment and gene-environment interactions in T1D pathogenesis. ...

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Номер записи: 31
02-05-2013 дата публикации

FAST SCREENING OF CLONES

Номер: US20130109041A1
Автор: HELD Martin, Meyer Andreas Jörg, PANKE Sven, Pellaux Rene, Walser Marcel
Принадлежит:

The invention relates to a method of fast identification and isolation of cells featuring a desired phenotype. The phenotype is coupled to the amount of gas-liberating enzymes or increased growth. The cells are encapsulated into microcapsules allowing exchange of solvents through the microcapsule wall, but retaining some or all of the gas formed by gas-liberating enzymes on contact with corresponding substrates. Microcapsules containing increased amounts of gas-liberating enzymes are starting to float and can be separated. The cells are then isolated from the microcapsules according to standard procedures. 1. A method of fast identification and isolation of cells featuring a particular phenotype , comprising(a) optionally coupling the expression of the desired particular phenotype to the amount of gas-liberating enzymes in the cells and/or to increased growth of the cells;(b) encapsulating the cells into microcapsules allowing exchange of solvents through the microcapsule wall but retaining all or a fraction of the gas formed by gas-liberating enzymes;(c) culturing the microcapsules harbouring the cells under conditions supporting the expression of the particular phenotype and the formation of gas-liberating enzymes;(d) incubating the microcapsules in a reaction liquor containing a dissolved substrate for the gas-liberating enzymes until gas bubbles are formed in a fraction of the microcapsules;(e) separating microcapsules floating on the reaction liquor due to the reduction of their specific density caused by gas bubble formation, optionally in a time-dependent manner; and(f) isolating the cells from the separated microcapsules.2. The method of wherein in step (a) the expression of a desired particular phenotype is coupled to the amount of gas-liberating enzymes.3. The method of wherein in step (a) the expression of a desired particular phenotype is coupled to increased growth of the cells.4. The method of wherein in step (a) the expression of a desired phenotype ...

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Номер записи: 32
02-05-2013 дата публикации

METHOD FOR DETECTING MICROORGANISMS, DEVICE FOR DETECTING MICROORGANISMS AND PROGRAM

Номер: US20130109051A1
Автор: Kai Chizuka, Li Shenglan, Nishida Takashi, Toyoshima Kunimitsu
Принадлежит:

A method for detecting microorganisms, which comprises: a training step for forming, by a classifier, feature vectors based on color data on individual points within a subject region of training in a culture medium, mapping the points in the culture medium, that are specified by the feature vectors, on a high-dimensional feature space, and linearly separating a set of the points ψ (x), that are specified by the high-dimensional feature vectors thus obtained, to thereby color-classify the class (C) of the culture medium; and a identifying step for forming, by a classifier, feature vectors based on color data on individual inspection points within a region in the culture medium using image data obtained by capturing an image of the culture medium under cultivation, mapping the inspection points (xj), that are specified by the feature vectors, on a high-dimensional feature space, and determining whether or not the mapped points ψ (xj), that are specified by the high-dimensional feature vectors thus obtained, belong to the class (C) of the culture medium, thereby identifying a colony based on inspection points not belonging to the class (C) of the culture medium. 1. A microorganism detecting method for detecting a microbial colony cultivated in a medium , the method comprising a training step and an identifying step , whereinthe training step includes:capturing a color image of a learning subject with or without a microbial colony within a medium region, setting at least a part of the medium region as a training subject region within the captured color image;obtaining, as learning data, color data of either or both of medium points and microorganism points within the training subject region;supplying the learning data to a classifier to obtain feature vectors of the color data; andseparating, by the classifier, a set of points specified by the feature vectors to classify at least one of a class of medium and a class of microorganisms, andthe identifying step includes: ...

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Номер записи: 33
02-05-2013 дата публикации

STERILITY TEST METHOD AND TOTALLY ENCLOSED BACTERIAL AMPOULE INCUBATOR USED BY IT

Номер: US20130109052A1
Автор: Jin Cheng, Ren Yongshen, Xiao Xiaohe, YAN Dan, Zhang Ping
Принадлежит: 302 MILITARY HOSPITAL OF CHINA

A sterility test method includes: selecting strain and culture medium, preparing bacterial cultures, transcribing fingerprint characteristics in thermograms as indices to verify the characteristics, drawing the thermodynamic parameters of the thermogram, determining the positive judgment index and performing sterility test for the samples. A fully-enclosed bacteria collecting ampoule incubator includes bacteria collecting ampoule system, sample and liquid feeding system and peristalsis liquid discharge system. The sample and liquid feeding system is connected with the bacteria collecting ampoule system by the liquid intake tube; and the bacteria collecting ampoule system is connected with the peristalsis liquid discharge system by the liquid drainage tube. The invention is characterized by short inspection time, high sensitivity, high automation and accurate test results on microbial contamination. It can also provide the overall process curve on the growth conditions. Such curve is provided with relatively favorable fingerprint, which enables qualitative analysis on the microbial contamination conditions. 1. A sterility test method , characterized in that the method comprises the followings steps:(1) preparing bacteria cultures: cultivating different bacteria strains in sterile a culture medium to obtain bacteria cultures with different concentrations and different survival conditions for the different bacteria strains as positive controls for recording fingerprint characteristics of thermograms of the different bacteria strains; wherein the method for obtaining the bacteria cultures of different concentrations for the different bacteria strains comprises: washing fresh bacteria cultures to obtain eluents, diluting the eluents with 0.9% sterile sodium chloride solution to produce a series of 10-fold dilutions; wherein the method for obtaining the bacteria cultures of different survival conditions for the different bacteria strains comprises: filtering and eluting ...

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Номер записи: 34
09-05-2013 дата публикации

METHOD FOR PRODUCING EMBRYOS BY IN VITRO CULTURE, AND METHOD, APPARATUS, AND SYSTEM FOR SELECTING EMBRYOS

Номер: US20130116499A1
Автор: Aikawa Yoshio, Akai Tomonori, Imai Kei, Ohtake Masaki, SUGIMURA Satoshi
Принадлежит: Dai Nippon Printing Co., Ltd.

An object of the present invention is to provide a means for efficiently obtaining mammalian embryos having high conception rates. A first aspect of the present invention is a method for selecting a mammalian embryo prepared by in vitro culture from a fertilized egg, comprising a step of selecting an embryo using two or more of the following indicators: the time from fertilization to the completion of first cleavage; the morphology at a stage after first cleavage and before second cleavage; the morphology at a stage after third cleavage and before fourth cleavage; and the amount of oxygen consumed at the early blastocyst stage, the blastocyst stage, or the expanded blastocyst stage. 1. A method for selecting a mammalian embryo prepared by in vitro culture from a fertilized egg , comprising a step of selecting an embryo using two or more of the following indicators:the time from fertilization to the completion of first cleavage;the morphology at a stage after first cleavage and before second cleavage;the morphology at a stage after third cleavage and before fourth cleavage; andthe amount of oxygen consumed at the early blastocyst stage, the blastocyst stage, or the expanded blastocyst stage.2. The method according to claim 1 , wherein the embryo selected in the step satisfies two or more of the following conditions:at least one condition selected from the following condition “d1” to “d3” is satisfied (condition “d”): the time from fertilization to the completion of first cleavage of an embryo is confirmed to result in a probability of 40% or more that the chromosome number of the embryo at the blastocyst stage will be normal, based on the correlation between the time from fertilization to the completion of first cleavage of the embryo and the probability (condition “d1”), the time from fertilization to the completion of first cleavage of an embryo is confirmed to result in an expression level of 0.45 or more of an IGF2R gene in the embryo at the blastocyst stage ...

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Номер записи: 35
16-05-2013 дата публикации

SELECTION AND USE OF HOST CELLS FOR PRODUCTION OF GLYCOPROTEINS

Номер: US20130123126A1
Автор: Bhatnagar Naveen, Bosques Carlos J., Collins Brian Edward, Duffner Jay, Farutin Victor, Kaundinya Ganesh, Thiruneelakantapillai Lakshmanan
Принадлежит: MOMENTA PHARMACEUTICALS, INC.

A method of making a glycoprotein having a selected glycostructure. 1. A method of making a glycoprotein having a selected glycan complement or glycan component comprising:(a) acquiring the identity of a cell population for the production of said glycoprotein, wherein the identity is acquired or determined by(i) acquiring, for each of a plurality of isolates or aliquots of a first cell population, a value which is expressed in terms of a glycan complement or glycan component, which value is a function of a plurality of distinct observations that include the level of expression of a plurality of different genes and the level of expression of a plurality of different glycostructures, glycan structures, glycan components, or combinations thereof to provide a set of values for said first cell population;(ii) acquiring, for each of a plurality of isolates or aliquots of a second cell population, a value which is expressed in terms of a glycan complement or glycan component, which value is a function of a plurality of distinct observations that include the level of expression of a plurality of different genes and the level of expression of a plurality of different glycostructures, glycan structures, glycan components, or combinations thereof to provide a set of values for said second cell population;(iii) comparing a value for a selected glycan complement or glycan component with the set of values for said first cell population and with the set of values for said second cell population; and(iv) responsive to said comparison, selecting said first or second cell population; and(b) culturing said selected cell population, to thereby make said glycoprotein having said selected glycan complement or glycan component.2. The method of claim 1 , further comprising isolating said glycoprotein from the culture.3. The method of claim 2 , further comprising purifying said glycoprotein.4. The method of claim 1 , further comprising(i) acquiring a cell population quality attribute ...

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Номер записи: 36
23-05-2013 дата публикации

Microfluidics Sorter For Cell Detection And Isolation

Номер: US20130130226A1
Автор: Bhagat Ali Asgar, Han Jongyoon, Hou Han Wei, Lee Wong Cheng, Lim Chwee Teck, Vliet Krystyn J. Van
Принадлежит:

A method of detecting one or more diseased blood cells in a blood sample includes introducing a blood sample into at least one inlet of a microfluidic device comprising one or more linear channels wherein each channel has a length and a cross-section of a height and a width defining an aspect ratio adapted to isolate diseased blood cells along at least one portion of the cross-section of the channel based on reduced deformability of diseased blood cells as compared to non-diseased blood cells, wherein diseased blood cells flow along a first portion of the channel to a first outlet and non-diseased blood cells flow along a second portion of the channel to a second outlet. The one or more channels can be adapted to isolate cells along portions of the cross-section of the channel based on cell size. In some embodiments, the one or more channels can be spiral channels. 1. A method of detecting one or more diseased blood cells in a blood sample , comprising introducing the blood sample into at least one inlet of a microfluidic device comprising one or more linear channels wherein each linear channel has a length and a cross-section of a height and a width defining an aspect ratio adapted to isolate diseased blood cells along at least one portion of the cross-section of the channel based on reduced deformability of diseased blood cells as compared to non-diseased blood cells , wherein diseased blood cells flow along a first portion of the channel to a first outlet and non-diseased blood cells flow along a second portion of the channel to a second outlet.2. The method of claim 1 , wherein the diseased cells are of a different size claim 1 , stiffness claim 1 , deformability claim 1 , adhesiveness claim 1 , or a combination thereof than the non-diseased cells.37-. (canceled)8. The method of claim 2 , wherein the one or more diseased cells are malaria-infected red blood cells claim 2 , sickle cell anemia red blood cells claim 2 , leukemic red blood cells claim 2 , ...

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Номер записи: 37
23-05-2013 дата публикации

METHODS FOR SCREENING MICROBIAL REMEDIATION AGENTS

Номер: US20130130303A1
Автор: Dixit Anupma, Tumala Brunda
Принадлежит: Saint Louis University, a non-profit organization

Disclosed are methods for determining the efficacy of antimicrobial agents used in the treatment of building materials after microbial contamination has occurred, for the purpose of killing existing microbial growth and reducing or inhibiting recurrent or subsequent microbial growth. The disclosed methods may be used to determine microbial growth at time points subsequent to antimicrobial treatment of the material surface. The disclosed invention also measures visible microbial growth in a semi-quantitatively analysis. In addition, the Inventors disclosure a method with reduced variability and a more accurate assessment of antimicrobial efficacy. 1. A method of determining the effectiveness of an antimicrobial agent in reducing or inhibiting recurrent microbial growth , the method comprising:a) obtaining a substrate with visible substantial microbial growth or gross contamination,b) removing the visible substantial microbial growth or gross contamination from the substrate by mechanical cleaning,c) treating the substrate with the antimicrobial agent,d) incubating the substrate for one or more periods of time at a temperature and relative humidity that will allow growth of the microbe,e) determining viable colony forming units by extracting viable microbes from the substrate and plating the extract on nutritive media, then incubating the viable microbes on the nutritive media in an environment that allows growth of the viable microbes and,f) comparing the viable colony forming units obtained from the antimicrobial treated substrates, to viable colony forming units obtained from substrates not treated with an antimicrobial, or to viable colony forming units obtained from substrates treated with different antimicrobials and subjected to steps a) through e).2. The method of claim 1 , wherein incubating the substrate for one or more periods of time to determine efficacy of the antimicrobial claim 1 , comprises incubating the substrate for a period of time determined to ...

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Номер записи: 38
23-05-2013 дата публикации

METHODS FOR SCREENING MICROBIAL GROWTH INHIBITION ACTIVITY ON MATERIALS

Номер: US20130130304A1
Автор: Dixit Anupma, Tumala Brunda
Принадлежит: Saint Louis University, a non-profit organizaton

Disclosed are methods of determining the effectiveness of an antimicrobial agent in reducing or inhibiting microbial growth on a substrate. The disclosed methods may be used to determine microbial growth at time points subsequent to antimicrobial treatment of the material surface and exposure to microorganisms. The disclosed invention also measures visible microbial growth using a semi-quantitatively method that is more accurate and less subjective than estimates of growth used previously. The disclosed method that provides an accurate assessment of antimicrobial efficacy with reduced variability. 1. A method of determining the effectiveness of an antimicrobial agent in reducing or inhibiting microbial growth on a substrate , the method comprising:a) treating a substrate with the antimicrobial agent,b) evenly dispersing a volume of a microbial suspension of known concentration on the top surface of the substrate,c) placing the substrate in a sterile culture container,d) incubating the substrate for one or more periods of time at a temperature and relative humidity that will allow growth of the microbe,e) determining viable colony forming units by extracting viable microbes from the substrate and plating the extract on nutritive media and incubating the extract on nutrient media under conditions which allow growth of the microbe and,f) comparing the viable colony forming units obtained from substrates treated with the antimicrobial agent to viable colony forming units obtained from substrates not treated with an antimicrobial agent or to colony forming units obtained from substrates treated with a different antimicrobial agent and subjected to steps a) through e).2. The method of claim 1 , wherein claim 1 , incubating the substrate for one or more periods of time to determine efficacy of the antimicrobial claim 1 , comprises incubating the substrate for a period of time determined to represent short term efficacy.3. The method of claim 2 , wherein the time determined ...

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Номер записи: 39
23-05-2013 дата публикации

CELL OBSERVATION DEVICE AND CELL OBSERVATION METHOD

Номер: US20130130307A1
Автор: Ikeda Takahiro, Kataoka Takuji, Sugiyama Norikazu
Принадлежит: HAMAMATSU PHOTONICS K.K.

A cell observation device is provided with a reflection interference shutter A which adjusts a light quantity of light emitted from a reflection interference measurement light source , a quantitative phase shutter A which adjusts a light quantity of light emitted from a quantitative phase measurement light source, a camera which images reflected light from the reflection interference measurement light source to generate a reflection interference image and which images transmitted light from a quantitative phase measurement light source to generate a quantitative phase image, and a first extraction unit and a second extraction unit which extract first and second parameters from the reflection interference image generated by the camera . During generation of the reflection interference image, the quantitative phase shutter A blocks the light from the quantitative phase measurement light source During generation of the quantitative phase image, the reflection interference shutter A blocks the light from the reflection interference measurement light source 1. A cell observation device comprising:a reflection interference measurement light source;reflection interference light quantity adjustment unit which adjusts a light quantity of light emitted from the reflection interference measurement light source;a quantitative phase measurement light source;quantitative phase light quantity adjustment unit which adjusts a light quantity of light emitted from the quantitative phase measurement light source;imaging unit which images reflected light from a cell, of the light emitted from the reflection interference measurement light source, to generate a reflection interference image, and which images transmitted light through the cell, of the light emitted from the quantitative phase measurement light source, to generate a quantitative phase image;first extraction unit which extracts a first parameter from the reflection interference image generated by the imaging unit; andsecond ...

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Номер записи: 40
23-05-2013 дата публикации

INTEGRATED MICROBIAL COLLECTOR

Номер: US20130130368A1
Автор: Babico John Y., Jiang Jian-Ping
Принадлежит: BIOVIGILANT SYSTEMS, INC.

A system for real-time sizing of fluid-borne particles is disclosed. The system further determines, in real time, whether the detected particles are biological or non-biological. As the fluid is being tested, it is exposed to a microbe collection filter which is cultured to determine the type of microbes present in the fluid being tested. 1. A microbial detection and identification system , comprising:a housing, including a sampling area having a fluid, a light source, a first optical detector and a second optical detector;a blower in fluid communication with said housing, wherein said blower supplies negative pressure to said housing thereby drawing environmental air into said sampling area and evacuating air from said sampling area; anda microbe collection filter in fluid communication with said sampling area, wherein environmental air is drawn by the blower from said sampling area through the microbe collection filter.2. The system of claim 1 , wherein said sampling area is defined by an input nozzle and an output nozzle.3. The system of claim 2 , wherein said microbe collection filter includes a front side facing said exit nozzle claim 2 , a rear side in fluid communication with said blower claim 2 , and a gas permeable interior allowing said fluid to flow into said front side and out of said rear side.4. The system of claim 1 , wherein claim 1 ,said light source illuminates particles in said sampling area,said first detector detects light scattered into a predetermined angular range by particles of a predetermined size,said second detector measures light emitted by fluorescence from illuminated biological particles in said sample area,and said fluid is exposed to said microbe collection filter causing particles in said fluid to adhere to said microbe collection filter.5. The system of claim 4 , further comprising scattered light collection components that direct scattered light from said sampling area to said first detector and a plurality of optical components ...

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Номер записи: 41
30-05-2013 дата публикации

Use of a beta-glucosidase activator for the detection and/or identification of c. difficile

Номер: US20130137126A1
Автор: Diane Halimi, Marie Cellier, Sylvain Orenga
Принадлежит: bioMerieux SA

The present invention relates to a reaction medium comprising at least one beta-glucosidase substrate and a compound of the general formula Ar-beta-D-glucoside where Ar- designates an aromatic compound, different from said substrate. According to the invention, such a medium can be employed in a C. difficile detection and/or identification process.

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Номер записи: 42
06-06-2013 дата публикации

Method for Detection of Inflammation in the Urinary Tract or Urethra

Номер: US20130143252A1
Автор: Knight Jan, Knight Robert
Принадлежит: Knight Scientific Limited

A method for detecting inflammation in the urinary tract or urethra of a patient, especially urethritis, comprises a) contacting leucocytes obtained from a urine sample provided by the patient with a luminescence reagent which emits light on reaction with an oxidant; b) adding an activator to the mixture of leucocytes and luminescence reagent; c) continuously monitoring and/or measuring light emitted by the luminescence reagent over a predetermined time period commencing before and ending after the addition of the activator. The light emission is indicative of the presence or absence of inflammation in the urinary tract or urethra of the patient. The urine sample is preferably a sample of first pass urine. The method makes possible a diagnosis especially of urethritis and, in particular, urethral infections selected from and , which can be carried out quickly without invasive procedures. A diagnostic kit for carrying out the method, comprising a luminescence reagent which emits light on reaction with an oxidant, an activator and a library of standard signature light emission curves is also disclosed. 1. A method for detecting inflammation in the urinary tract or urethra of a patient , which method comprisesa) contacting leucocytes obtained from a urine sample provided by the patient with a luminescence reagent which emits light on reaction with an oxidant;b) adding an activator to the mixture of leucocytes and luminescence reagent;c) continuously monitoring and/or measuring light emitted by the luminescence reagent over a predetermined time period commencing before and ending after the addition of the activator, this light emission being indicative of the presence or absence of inflammation in the urinary tract or urethra of the patient.2. A method according to for detecting urethritis in a patient comprisinga) contacting urethral leucocytes obtained from a urine sample provided by the patient with a luminescence reagent which emits light on reaction with an oxidant ...

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Номер записи: 43
13-06-2013 дата публикации

METHODS OF IDENTIFYING THERAPEUTIC AGENTS FOR TREATING PERSISTER AND BACTERIAL INFECTION

Номер: US20130149701A1
Автор: Shi Wanliang, ZHANG YING
Принадлежит:

The present invention relates to methods, compositions, assays and kits for identifying an antibacterial agent that decreases persister formation or survival, eliminates or reduces bacterial infection or disease and/or increases killing of a bacterial cell. 1. A method of screening for an antibacterial agent that decreases persister formation or survival , eliminates or reduces bacterial infection or disease and/or increases killing of a bacterial cell , comprising the steps of:(a) contacting a test agent with a composition comprising a Pyrazinamide (PZA)-sensitive target protein; andb) determining whether the test agent binds to, or inhibits activity of, the target protein,wherein binding of the target protein or inhibition of the target protein activity is indicative of a potential antibacterial agent for decreasing persister formation or survival, eliminating or reducing bacterial infection or disease and/or increasing killing of a bacterial cell.2. The method of claim 1 , wherein the agent binds to claim 1 , and inhibits the activity of claim 1 , the target protein.3M. tuberculosis;. The method of claim 1 , wherein the target protein is selected from one or more of Endonuclease IV (Rv0670) Nfo (end) involved in DNA repair; Polynucleotide phosphorylase (PNPase) (Rv2783c) GpsI; Iron-regulated heparin-binding hemagglutinin (Rv0475) HbhA involved in extra-pulmonary dissemination of 30S ribosomal protein S1 (Rv1630) RpsA; 30S ribosomal protein S4 (Rv3458c) RpsD; 50S ribosomal protein L9 (Rv0056) RplI; 50S ribosomal protein L10 (Rv0651) RpIJ; 50S ribosomal protein L7/L12(Rv0652) RpIL; 50S ribosomal protein L29 (Rv0709) RpmC; Putative DNA repair ATPase (Rv2731) and Hypothetical protein MT3258 (Rv3169).4. The method of claim 1 , wherein the target protein is a protein inhibited by Pyrazinamide (PZA) or pyrazinoic acid (POA).5. The method of claim 1 , wherein the target protein is a protein that binds claim 1 , to or is inhibited by active component of PZA claim 1 , ...

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Номер записи: 44
13-06-2013 дата публикации

MICROBIAL DETECTION SYSTEM AND METHODS

Номер: US20130149738A1
Автор: BOLEA PHILLIP A., HALVERSON KURT J., Zook Cynthia D.
Принадлежит: 3M INNOVATIVE PROPERTIES COMPANY

The disclosure provides culture devices and methods for a microorganism in a sample. The devices include a base member, a cover sheet, an adhesive layer coupled to the base member or the cover sheet, and a cold water-soluble gelling agent disposed on the base member; wherein the devices are substantially optically transmissive when the gelling agent is hydrated with a clear liquid. Methods of use include detecting or enumerating microorganisms. The methods further provide for detecting a microorganism by detecting the presence or size of an abiogenic gas bubble () in a culture device. 110-. (canceled)11. A method for detecting the presence or absence of a microorganism in a sample , comprising: wherein the culture device comprises an outermost first major surface and an outermost second major surface;', 'wherein the hydrogel defines a growth area;, 'providing a sample and a culture device comprising a base member, a cover layer, and a hydrogel comprising a plurality of abiogenic gas bubbles disposed there between;'}inoculating the growth area of the device with the sample at a first point in time;incubating the device for a period of time;illuminating the growth area with a light source; anddetecting the presence or absence of a microorganism in the growth area at a second point in time;wherein detecting the presence or absence of a microorganism comprises observing an indication of growth;wherein observing an indication of growth comprises detecting the diminution or absence of at least one abiogenic gas bubble in the hydrogel at the second point in time.12. The method of ; wherein providing the culture device comprises providing a thin film culture device that includes a dry claim 11 , cold water-soluble gelling agent and wherein the method further comprises hydrating the gelling agent with an aqueous liquid.13. The method of claim 11 , further comprising:observing the growth area with regard to the size or absence of the gas bubble at a third point in time, ...

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Номер записи: 45
13-06-2013 дата публикации

Detection and enumeration of microorganisms

Номер: US20130149739A1
Автор: Adrien Ducret, Marina Periame, Sam Dukan, Yannick Fovet
Принадлежит: BASF SE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms: (1) contacting said microorganisms of said sample with repair compounds and a growth medium, and (2) incubating the product of step (1), and (3) detecting and enumerating said microorganisms, in which the microorganisms are of the species Legionella pneumophila , and in which the repair compounds comprise: (a) serine; (b) threonine; (c) a compound containing calcium ions at a dose of 10 −6 to 10 −2 mM; (d) a compound containing magnesium ions at a dose of 10 −6 to 10 −2 mM; (e) a compound containing potassium ions; (f) glutamic acid or a salt thereof; and (g) pyruvic acid or a salt thereof. The invention also discloses a kit for detecting and enumerating viable microorganisms of the species Legionella pneumophila in a sample suspected of containing said microorganisms.

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Номер записи: 46
20-06-2013 дата публикации

Method for Determining the Presence of an Analyte by Means of Small Magnetic Particles, and Corresponding Device

Номер: US20130157256A1
Автор: Behr Volker C., Kampf Thomas, Rueckert Martin
Принадлежит:

The invention relates to a method for determining the presence of an analyte by means of a distribution of small magnetic particles. According to said method, the magnetisations of the small particles are oriented in relation to each other by means of an outer magnetic focusing field; once the focussing field has been terminated, the magnetisations of the small particles are rotated asynchronously to the magnetic field by means of an outer magnetic field of suitable field intensity and rotational frequency, which rotates about a longitudinal axis (z); the temporal course of the superpositioned transverse magnetisation of the set of particles is detected; and the presence of the analyte is deduced from the detected temporal course. The invention also relates to a corresponding device (). 1. A method for determining the presence of an analyte using a distribution of small magnetic particles , whereinthe magnetizations of the small particles are aligned with one another using an external magnetic focusing field,after the focusing field has been switched off, the magnetizations of the small particles are set into a rotation which is asynchronous with the magnetic field using an external magnetic field of suitable field strength and rotation frequency that rotates about a longitudinal axis (z),the temporal profile of the superposed transverse magnetization of the particle ensemble is detected, andconclusions relating to the presence of the analyte are drawn from the detected temporal profile.2. The method as claimed in claim 1 ,wherein the temporal profile of the transverse magnetization is detected only with a delay time after the focusing field has been switched off.3. The method as claimed in claim 1 ,wherein the temporal profile of the transverse magnetization is detected only after a dead time after the focusing field has been switched off.4. The method as claimed in claim 1 ,wherein the time derivation is formed from the profile of the transverse magnetization, and ...

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Номер записи: 47
20-06-2013 дата публикации

RAPID PATHOGEN DIAGNOSTIC DEVICE AND METHOD

Номер: US20130157283A1
Автор: Cooper Ryan Mcomber, Domansky Karel, Ingber Donald E., Leslie Daniel Christopher, Super Michael, Vollmer Frank, Yung Chong Wing
Принадлежит: President and Fellows of Harvard College

A microfluidic device of a diagnostic and detection system includes an inlet port connected by one or more microchannels to an outlet port and includes a capture and visualization chamber (CVC) connected to at least one microchannel. A fluid to be analyzed can be mixed with magnetic microbeads that have an affinity to become bound to target components, such as pathogens in the fluid. The fluid including the magnetically bound target components can be injected through the microfluidic device. Magnetic field gradient, such as provided by permanent or electro-magnets, can be applied to the fluid and the magnetically bound target components flowing through the microfluidic device to cause the magnetically bound target components to migrate into the (CVC) and become separated from the fluid. The magnetically bound target components can be analyzed and tested using various techniques to detect the presence of specific organic and inorganic materials, such as pathogens in bio-fluids and contamination in liquid food sources (e.g. water). The device and method provide a system for rapidly detecting pathogens and contamination in relatively small fluid samples. 1. A microfluidic device comprising:an inlet port adapted to be connected to a fluid source;an outlet port adapted to be connected to a fluid receiver;at least one microchannel connected to and extending between the inlet port and the outlet port;a capture chamber connected to the microfluidic channel, the capture chamber including at least one feature adapted to capture target components flowing in a source fluid provided by the fluid source; anda magnetic source above the microchannel and configured to apply a magnetic field gradient to the source fluid flowing through the microchannel and to cause magnetic microbead bound target components in the source fluid to migrate into the capture chamber.2. The microfluidic device of claim 1 , wherein the microfluidic device further comprising a magnetic concentrator between ...

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Номер записи: 48
20-06-2013 дата публикации

Diagnosis of bacterial meningitis based on the measure of ROS production in a sample of cerebrospinal fluid

Номер: US20130157301A1
Автор: Lukaszewicz Anne-Claire, Ouanounou Ingrid, Payen De La Garanderie Didier
Принадлежит: ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS

The present invention pertains to a method for in vitro diagnosing a bacterial infection in a biological fluid selected amongst cerebrospinal fluid, ascitic fluid, pericardial fluid, pleural fluid, urine and synovial fluid, based on the measure, in a sample of said fluid, of the production of reactive oxygen species (ROS); a high level of ROS production is indicative of the presence of activated polymorphonuclear neutrophils (PMNs) in said fluid, which in turn is a hallmark of bacterial infection. 1. A method for in vitro diagnosing a leukocytosis and/or a bacterial infection in a biological fluid selected from the group consisting of cerebrospinal fluid , ascitic fluid , pericardial fluid , pleural fluid , urine and synovial fluid , comprising a step of measuring , in a sample of said fluid collected from a subject , the production of reactive oxygen species (ROS) , wherein a ROS production above a predetermined threshold is indicative of a leukocytosis and/or a bacterial infection.2. The method of claim 1 , comprising a step of measuring the level of ROS spontaneously produced by polymorphonuclear neutrophils (PMNs) present in the sample of biological fluid claim 1 , wherein a ROS production above a predetermined threshold is indicative of a bacterial infection in said biological fluid.3. The method of claim 1 , comprising a step of measuring the level of ROS produced by the PMNs present in the sample of biological fluid after stimulation by an agonist of NADPH oxidase claim 1 , wherein a ROS production above a predetermined threshold is indicative of a leukocytosis in said biological fluid.4. The method of claim 1 , wherein the measure of ROS production by the PMNs present in the sample of biological fluid is performed at most eight hours after sample collection.5. The method of claim 1 , wherein ROS production is measured in a sample of cerebrospinal fluid claim 1 , and wherein a spontaneous ROS production above a predetermined threshold is indicative of ...

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Номер записи: 49
20-06-2013 дата публикации

METHOD AND SYSTEM FOR EVALUATION OF GRAFTS

Номер: US20130157305A1
Автор: SAKAMOTO Kenta
Принадлежит: TERUMO KABUSHIKI KAISHA

A method and system for evaluating the competence for transplantation of a graft sample containing cells capable of multinucleation are provided. In one form, the method includes determining multinucleating ability as an indicator representing the capacity for multinucleation of the cells capable of multinucleation. 1. A method for evaluating the competence for transplantation of a graft sample containing cells capable of multinucleation , the method comprising:determination of multinucleating ability as an indicator representing the capacity for multinucleation of the cells capable of multinucleation,wherein the determination of the multinucleating ability comprises:(i) a step of dissociating a portion of one or not less than two graft samples into individual cells;(ii) a step of incubating the dissociated cells obtained in step (i); and(iii) a step of detecting multinucleate cells in a cell culture obtained upon the incubation in step (ii), andthe thus determined multinucleating ability is compared with a preset threshold value.2. The method as defined in claim 1 , wherein the cells capable of multinucleation are skeletal myoblasts.3. The method as defined in claim 1 , wherein the multinucleating ability is rate of multinucleation.4. The method as defined in claim 1 , wherein the graft sample is determined as competent for transplantation to a living body where the cells capable of multinucleation are skeletal myoblasts and the rate of multinucleation as the multinucleating ability to be determined is higher than a preset threshold value.5. A system for evaluation of competence for transplantation of a graft sample containing cells capable of multinucleation claim 1 , the system comprising:a detecting unit which detects multinucleate cells; anda calculating unit which calculates the multinucleating ability.6. The system as defined in claim 5 , wherein the detecting unit has an image analyzing unit which analyzes a cell image picked up.7. The system as defined in ...

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Номер записи: 50
20-06-2013 дата публикации

Method of Analyzing Cardiovascular Disorders and Uses Thereof

Номер: US20130157347A1
Автор: Connelly Mark, Damani Samir, Rao Galla Chandra, Topol Eric
Принадлежит:

Compositions, systems, and methods comprising circulating endothelial cells (CECs) are provided. The compositions described herein utilize isolated CECs, including compositions in a form that allows analysis of the CECs. The systems described herein utilize isolated CECs and an analytical tool or an output from an analytical tool. The methods described herein are related to the use of isolated CECs and analytical tools for providing information, to a health care provider or the CEC donor, that is relevant to the cardiovascular health of the CEC donor. Thus, the compositions, systems and methods described herein involve both a transformation (e.g., non-isolated CECs to isolated CECs, or isolated CECs to analyzed CECs) and a machine (e.g., isolation tools and analytical tools). 2. The system of claim 1 , further comprising electronic memory for capturing and storing a magnified image of the population of CECs or a single CEC in the population of CECs.3. The system of claim 1 , further comprising a computer-processing device claim 1 , optionally connected to a computer network.4. The system of claim 3 , further comprising a software module executed by the computer-processing device to analyze a morphological feature of the population of circulating endothelial cells (CEC)5. The system of claim 3 , further comprising a software module executed by the computer-processing device to compare the morphological feature of the population of circulating endothelial cells (CEC) to a standard or control.6. The system of claim 3 , further comprising a software module executed by the computer-processing device to determine concentration of the population of circulating endothelial cells isolated from the blood sample.7. The system of claim 6 , further comprising a software module executed by the computer-processing device to compare the concentration of the population of circulating endothelial cells to the concentration of control or standard.8. The system of claim 1 , further ...

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Номер записи: 51
27-06-2013 дата публикации

Use of bacterial beta-lactamase for in vitro diagnostics and in vivo imaging, diagnostics and therapeutics

Номер: US20130164221A1
Автор: Hexin Xie, James C. Sacchettini, Jeffrey D. Cirillo, Jianghong Rao
Принадлежит: Leland Stanford Junior University, TEXAS A&M UNIVERSITY SYSTEM

Provided herein are methods for detecting, quantifying, differentiating, diagnosing and imaging pathogenic bacteria or condition associated therewith using substrates for bacterial enzymes. Fluorescent, luminescent or colorimetric signals emitted by substrates or enzyme products in the presence of the bacteria are compared to controls to detect and locate the pathogenic bacteria. Provided is a method for screening therapeutic agents to treat the pathophysiological conditions by measuring a signal emitted from the substrates or products in the presence and absence of the potential therapeutic agent and a diagnostic method for detecting a mycobacterial infection in a subject by contacting biological samples with a substrate and imaging for signals emitted from a mycobacterial beta-lactamase product. Also provided are fluorogenic substrates or substrates comprising a colored dye or a chemical reagent effective to induce a color or pH change.

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Номер записи: 52
27-06-2013 дата публикации

Cellular Analysis of Body Fluids

Номер: US20130164740A1
Автор: Lin Emily H., Wu Jiong, Yang Jihping
Принадлежит: ABBOTT LABORATORIES

Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure. 1. A method for analyzing a body fluid containing cells , the method comprising:staining the body fluid with a fluorescent dye, wherein the fluorescent dye permeates a cell membrane and binds to a nucleic acid to form a dye complex within the cell;irradiating the stained body fluid with energy from an energy source; andmeasuring a fluorescence signal emitted by the dye complex in the stained body fluid.2. The method of claim 1 , wherein the body fluid comprises fewer than about 20 cells/μL.3. The method of claim 1 , wherein the body fluid comprises greater than about 20 cells/μL.4. The method of claim 1 , wherein the nucleic acid is a DNA or an RNA.5. The method of claim 1 , wherein the energy source produces monochromatic light having a wavelength in the visible spectrum claim 1 , and wherein the wavelength of the monochromatic light and the wavelength of the fluorescence signal are different.6. The method of claim 1 , wherein unbound fluorescent dye emits less fluorescent light when irradiated with energy from the energy source compared with the dye complex.7. The method of claim 1 , wherein unbound fluorescent dye does not fluoresce when irradiated with energy from the energy source while unbound to the nucleic acid claim 1 , such that cells lacking the ...

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Номер записи: 53
27-06-2013 дата публикации

METHOD USED IN A HUMAN OR ANIMAL FAECES SAMPLE PROCESSING SYSTEM, AND A SAMPLE PROCESSING SYSTEM

Номер: US20130164776A1
Автор: Hansson Lars Olof A, Spangeus Per
Принадлежит:

A sample processing system, and a method in relation thereto, adapted to automatically process a sample, e.g. a faecal sample, prior to the analysis of the sample. The system comprises a sample container for collecting a sample, and a control unit comprising a stored processing recipe that includes parameters to be used to automatically perform the processing of the sample. The control unit is adapted to generate robot control signals, obtained from said processing recipe, to be applied to a robot arranged to move the sample container between different units of the system and to generate control signals to a specific unit when the sample container is about to be treated by that unit. The robot is arranged to automatically move the sample container to: a weighing unit (optional) to determine the weight of the sample; a buffer supply unit to add buffer where the buffer amount can be predefmed or depend on the weight of the sample; a homogenization and mixing unit to homogenize and mix the sample in the buffer, preferably by using sonication by ultrasound. In addition the sample container is preferably moved to: a centrifuge to centrifuge the container in order to produce a clear supernatant; a transfer unit in order to transfer a part of the supernatant to a secondary container, the transferred volume is predefined, or related to the weight of the sample and/or related to the amount of buffer previously added; and if needed, the buffer supply unit adds buffer to the secondary container in order to dilute the sample solution to the desired final concentration. 1. Method of processing a human or animal faeces sample collected in a sample container , to be performed prior to the analysis of the sample , wherein the method comprises of automatically performing the following processing steps:A1: providing the sample container with a recipe code and a patient ID-code related to the sample and being arranged in connection to the sample container;A2: reading the recipe code ...

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Номер записи: 54
27-06-2013 дата публикации

Combination Assay Device and Method for Detecting Compounds in Vaginal Fluid

Номер: US20130164830A1
Автор: Martin Stephanie Michelle, Phillips Ronnie Lee
Принадлежит: KIMBERLY-CLARK WORLDWIDE, INC.

A combination assay device and method for simultaneously detecting the presence of hydrogen peroxide and D-lactic acid in vaginal fluid. The methods for detecting the hydrogen peroxide and the D-lactic acid are colorimetric-based. The device includes a pair of laminar flow substrates each having a solid-state compound for the detection of hydrogen peroxide and D-lactic acid disposed thereon. The solid-state format provides ease of use and storage. 1. The method of making an indicator of vaginal health comprising the steps of:combining a solid-state hydrogen peroxide indicator and a D-Lactic acid indicator together in a single housing so that there is no fluid communication therebetween;wherein the hydrogen peroxide indicator comprises a substrate having a side on which an amount of horseradish peroxidase and an amount of TMB dye are disposed in a spaced apart configuration;{'sup': '+', 'wherein the D-Lactic acid indicator comprises a substrate having a side on which an amount of D-LDH, NAD, diaphorase; and a NBT dye are disposed in a spaced apart configuration.'}2. The method of wherein the hydrogen peroxide indicator is configured to detect hydrogen peroxide at concentrations of 1 to 20 μM.3. The method of wherein the D-Lactic acid indicator is configured to detect D-Lactic acid at concentrations of about 1 mM.4. The method of further comprising the steps of dissolving the TMB dye in a 1:1 ratio of ethyl acetate and dimethyl sulfoxide; applying the dissolved TMB dye onto the substrate; and allowing the substrate to dry.5. The method of further comprising the step of dissolving the horseradish peroxidase in a PVA solution in a phosphate buffer.6. A combination solid-state assay device for monitoring vaginal fluid claim 1 , the device comprising:{'sup': '+', 'a first substrate having a surface upon which NBT dye and a solid-state D-Lactic acid detection composition are disposed in a spaced apart configuration, wherein the D-Lactic acid detection composition comprises ...

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Номер записи: 55
04-07-2013 дата публикации

Pharmaceutical composition for elevating radio-sensitivity of cancer cells, pharmaceutical composition for detecting cancer cells with radio-sensitivity, and detection method thereof

Номер: US20130171258A1
Автор: CHEN Chia-Chun, Chen Hai-Wen, SHIEH Dar-Bin, WU Ping-Ching
Принадлежит:

The present invention relates to a pharmaceutical composition for elevating radiation-sensitivity of cancer cells, which comprises: a nanoparticle containing with a first element, which is iron, copper, or the combination thereof; and a pharmaceutically acceptable carrier, wherein the nanoparticle is a metal nanoparticle, an alloy nanoparticle, or a metal nanoparticle with core-shell structure, and the size of the nanoparticle is under a controllable range of 3 nm to 150 nm. In addition, the present invention provides a detection method to detect radiation-sensitivity of the cancer cells through different modalities such as CT or MRI due to its native high CT number and magnetic property. Furthermore, the present invention provides a pharmaceutical composition for elevating radiation-sensitivity of the cancer cells through preferential uptake of the nanoparticle, in order to enhance the radiation-sensitivity of the cancer cells and improve the efficiency of radiation therapy to the cancer cells. 1. A pharmaceutical composition for detecting if a cancer cell is radiation resistant , comprising:a nanoparticle having a first element, wherein the first element is copper, platinum, or a combination thereof; anda medical acceptable carrier.2. The pharmaceutical composition according to claim 1 , wherein the nanoparticle is metal nanoparticle claim 1 , alloy nanoparticle claim 1 , or metal nanoparticle with a core-shell structure.3. The pharmaceutical composition according to claim 1 , wherein the nanoparticle further comprises a second element claim 1 , and the second element is at least one selected from a group consisting of iron claim 1 , cobalt claim 1 , palladium claim 1 , gold claim 1 , silver claim 1 , nickel claim 1 , gadolinium claim 1 , and silicon.4. The pharmaceutical composition according to claim 1 , wherein the diameter of the nanoparticle is 3 nm to 150 nm.5. The pharmaceutical composition according to claim 1 , wherein the cancer cell is solid state ...

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Номер записи: 56
04-07-2013 дата публикации

METHOD AND APPARATUS FOR CHARACTERIZING BIOLOGICAL OBJECTS

Номер: US20130171685A1
Автор: Schütze Karin, Schütze Raimund
Принадлежит: CELLTOOL GMBH

In order to quantitatively characterize biological objects, for example individual cells, a stimulus is applied to a biological object in a contactless fashion. A measurement and a further measurement are performed on the biological object in order to ascertain a response of the biological object to the stimulus, wherein the measurement and the further measurement comprise detecting Raman scattering on and/or in the biological object and/or capturing data using digital holographic microinterferometry (DHMI). The biological object is characterized according to a result of the measurement and is sorted if needed. The stimulus can be applied by means of a laser beam that creates optical tweezers or an optical trap, by means of ultrasonic waves or an electric or magnetic radio frequency field. 1. A method for characterizing a biological object , the method comprising:applying a stimulus to the biological object in a contactless fashion; a measurement which comprises a detection of Raman scattering is performed on the biological object before application of the stimulus, and', 'a further measurement which comprises a further detection of Raman scattering is performed on the biological object after or during application of the stimulus;, 'carrying out plural measurements on the biological object to ascertain a response of the biological object to the stimulus, wherein, to ascertain the response,'}performing a comparison of results of the measurement and the further measurement; andcharacterizing the biological object as a function of the comparison of the results of the measurements.2. The method according to claim 1 , wherein the measurement and the further measurement comprise both the detection of a Raman scattering and data acquisition by means of DHMI.3. The method according to claim 1 , wherein a Raman spectrum is captured in the measurement and a further Raman spectrum is captured in the further measurement claim 1 , wherein a difference spectrum between the Raman ...

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Номер записи: 57
04-07-2013 дата публикации

Development of a detection microsystem

Номер: US20130171687A1
Автор: Enric Robine, Stephane Moularat, Yael Joblin
Принадлежит: Centre Scientifique et Technique du Batiment CSTB

This invention relates to a device for detecting fungal contamination in an interior environment, including: a concentration module (MC); a separation module (MS) including a chromatographic microcolumn; and a detection module (MD), characterized in that it includes at least one first solenoid valve (E 3 ) upstream of the detection module (MD) enabling either to direct a flow containing target molecules toward the detection module (MD), or to direct a flow filtered by a first means for filtering (Tx 1 ), enabling the detection module (MD) to be cleaned when the flow does not contain the target molecules. The invention also relates to a control interface of the device.

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Номер записи: 58
04-07-2013 дата публикации

CAPTURE OF MICRO-ORGANISMS

Номер: US20130171689A1
Автор: Stanley Christopher John, Wilson Stuart Mark
Принадлежит: MICROSENS MEDTECH LIMITED

Micro-organisms, including fungi, viruses and bacteria such as and/or fragments of micro-organisms such as cell wall components present in an aqueous liquid are captured to a solid surface by adding to the liquid a sufficient quantity of a water soluble polymer in the presence of the solid surface to displace the micro-organisms and/or fragments from the liquid to the solid surface. The surface may be provided by a bead. The water soluble polymer may be polyethyleneglycol or polyvinylpyrrolidone. 1. A method of capturing onto a solid surface micro-organisms and/or fragments of micro-organisms present in an aqueous liquid , comprising adding to said liquid a sufficient quantity of a water soluble polymer in the presence of said solid surface to displace said micro-organisms and/or fragments from the liquid to the solid surface.2. A method as claimed in claim 1 , wherein the concentration of the added water soluble polymer in the aqueous liquid is from 2 to 40% w/v.3. A method as claimed in claim 2 , wherein said concentration is from 5-15% w/v.4. A method as claimed in claim 1 , in which the water soluble polymer is a non-ionic hydrophilic polymer.5. A method as claimed in claim 4 , in which the water soluble polymer is a dextran claim 4 , PVP or PEG.6. A method as claimed in claim 1 , wherein the water soluble polymer has a molecular weight of from 1 claim 1 ,000 to 20 claim 1 ,000.7. A method as claimed in claim 6 , wherein the water soluble polymer has a molecular weight of from 5 claim 6 ,000 to 13 claim 6 ,000.8. A method as claimed in claim 1 , wherein the ionic strength of the aqueous liquid is raised by the addition of a water soluble inorganic salt to from 150 mM to 6M.9. A method as claimed in claim 8 , wherein said salt concentration is from 0.25 to 2.5M.10. A method as claimed in claim 1 , wherein the solid surface is provided by beads.11. A method as claimed in claim 10 , wherein said beads are paramagnetic claim 10 , ferromagnetic or have a density such ...

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Номер записи: 59
04-07-2013 дата публикации

PROGNOSIS AND TREATMENT OF BREAST CANCER

Номер: US20130172430A1
Автор: Lisanti Michael P., Sotgia Federica
Принадлежит:

Methods of prognosis and monitoring of breast cancer include determining the level of one or more of the markers comprising asymmetric dimethyl arginine (ADMA), beta-hydroxybutyrate (BHB) and microRNA-31 (miR-31) in patient samples. An increased level is correlated with poor prognosis. Breast cancer is treated by administration of one or more inhibitors of ADMA and/or BHB. 1. A prognostic method for breast cancer in a subject comprising:(a) determining the level of at least one of the following in a test sample from a subject: (i) asymmetric dimethyl arginine; (ii) beta-hydroxybutyrate; and (iii) miR-31; andb) comparing the level of at least one of (i) asymmetric dimethyl arginine, (ii) beta-hydroxybutyrate, and (iii) miR-31 in said test sample to the level of in a control sample, wherein an elevated level of at least one of (i) asymmetric dimethyl arginine, (ii) beta-hydroxybutyrate, and (iii) miR-31 in said test sample relative to the level in said control sample is a prognostic indicator of the course of breast cancer disease in said subject.2. A method of monitoring the progression of breast cancer in a subject , said method comprising:(a) obtaining a first sample from a subject at a first time point and a second sample from said subject at a second time point;(b) determining the level of at least one of the following in said first and second samples: (i) asymmetric dimethyl arginine; (ii) beta-hydroxybutyrate; and (iii) miR-31; and(c) comparing the level of said at least one of (i) asymmetric dimethyl arginine, (ii) beta-hydroxybutyrate, and (iii) miR-31 in said first sample to the level in said second sample, wherein an elevated level of at least one of (i) asymmetric dimethyl arginine, (ii) beta-hydroxybutyrate, and (iii) miR-31 in said second sample relative to the level in said first sample is an indication that the cancer has progressed in said subject.3. The method of or wherein the level of asymmetric dimethyl arginine is determined.4. The method of or ...

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Номер записи: 60
11-07-2013 дата публикации

METHOD OF CANCER DIAGNOSIS USING THE ANALYSIS OF ISOPOPES

Номер: US20130177936A1
Автор: Choi Won Cheol
Принадлежит:

Disclosed is a method of diagnosing cancer on the basis of the quantitative analysis of blood or tissue isotopes. The method can accurately diagnose cancer even when it is too small for current conventional technology to diagnose. 1. A method of diagnosing cancer , comprising measuring levels of isotopes of an element in a blood sample or a tissue sample.2. The method according to claim 1 , wherein the element is selected from a group consisting of hydrogen claim 1 , oxygen claim 1 , magnesium claim 1 , calcium claim 1 , potassium claim 1 , sulfur claim 1 , chloride claim 1 , silicon claim 1 , iron claim 1 , copper claim 1 , and combinations thereof.3. The method according to claim 1 , wherein the method is based on an increase in the level of deuterium (H) by 10% or higher compared to a normal standard.4. The method according to claim 1 , wherein the method is based on an increase in the level of O by 10% or higher compared to a normal standard.5. The method according to claim 2 , wherein the method is based on an increase in the level of a heavy isotope of the element compared to a normal standard.6. The method according to claim 1 , wherein the method is based on the depletion of K and/or S from the sample. The present invention relates, in general, to cancer diagnosis and, more particularly, to a method for diagnosing cancer on the basis of quantitative analysis of isotopes of blood or tissue samples, which is able to accurately detect cancer even when it is too small to be detected with conventional technologies.Isotopes are any of several different forms of an element each having a different atomic mass (mass number). The term “isotope”, coined by British chemist F. Soddy in 1913, comes from the Greek isos “equal”+topos “place,” because despite the different atomic weights, the various forms of an element occupy the same place on the periodic table.Generally, the chemical properties of an element depend on the number of protons, that is, the atomic number. ...

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Номер записи: 61
11-07-2013 дата публикации

COLLECTION BROTHS FOR MICROORGANISMS

Номер: US20130177938A1
Автор: Ward N. Robert
Принадлежит:

A collection broth used for recovering microorganisms on a surface is provided. The collection broth includes a buffering system and a neutralization system. All components of the collection broth are both food-grade and hypoallergenic. 1. A collection broth used for recovering microorganisms on a surface , the collection broth comprising a buffering system and a neutralization system , wherein all components of the collection broth are both food-grade components and hypoallergenic components.2. The collection broth of claim 1 , wherein the food-grade components are listed as generally recognized as safe (GRAS) direct food additives in section 21 of the U.S. Code of Federal Regulations claim 1 , or are included on the U.S. Food and Drug Administration's list of everything added to food in the United States (EAFUS).3. The collection broth of claim 1 , wherein the hypoallergenic components do not include components derived from milk claim 1 , eggs claim 1 , fish claim 1 , crustacean and molluscan shellfish claim 1 , tree nuts claim 1 , peanuts claim 1 , wheat claim 1 , soybean claim 1 , buckwheat claim 1 , celery claim 1 , lupin claim 1 , mustard claim 1 , and sesame.4. The collection broth of claim 1 , wherein the buffering system results in a pH in the range of 6 to 8 when the collection broth is mixed with high-acid sanitizers claim 1 , including peracetic acid type sanitizers employed claim 1 , at typical use concentrations.5. The collection broth of claim 1 , wherein the buffering system comprises anhydrous potassium phosphate dibasic and anhydrous potassium phosphate monobasic.6. The collection broth of claim 5 , wherein the buffering system comprises anhydrous potassium phosphate dibasic at a concentration from about 1 grams/liter to about 15 grams/liter and anhydrous potassium phosphate monobasic at a concentration from about 1 gram/liter to about 15 grams/liter.7. The collection broth of claim 1 , wherein the neutralization system is capable of neutralizing ...

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Номер записи: 62
11-07-2013 дата публикации

Flow Cytometer

Номер: US20130177973A1
Автор: Kondo Yasushi
Принадлежит: SHIMADZU CORPORATION

A flow cytometer for a microanalysis and a fast detection is provided. In the flow cytometer, the downstream portion of a flow cell is fitted with an optical detection system including a laser emitter and a light detector, an imaging system including a high-speed camera and a stroboscopic lamp, and a cell sorter. The high-speed camera and the stroboscopic lamp illuminates and photographs candidate particles based on a photographing trigger signal provided by a trigger generator. The trigger signal is provided by the trigger generator when a predetermined time has elapsed from the moment when an examination particle determined to be a candidate particle has been measured by the optical detection system. The high-speed camera photographs multiple images for a given period of time from the moment when the trigger signal is provided, and sends the image data to a target particle detection unit of a data processor. 1. A flow cytometer for detecting target particles having a predetermined morphology from among examination particles , comprising:a flow path through which the examination particles flow;a flow rate controller for controlling a flow rate of the examination particles flowing through the flow path;a light emitter for emitting light onto a predetermined detection area in the flow path;a light detector for detecting light from the detection area;a candidate particle determiner for determining whether or not the examination particles flowing through the detection area are candidate particles having optical properties of the target particles based on an output from the light detector;a photographing unit for taking an image of a predetermined photographing area which is downstream of the detection area in the flow path;a photographing timing instructor for instructing the photographing unit of timings for taking the image of the candidate particles flowing through the photographing area, based on a flow path length between the detection area and the photographing ...

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Номер записи: 63
11-07-2013 дата публикации

METAPROTEOMIC METHOD FOR DIAGNOSIS OF BACTERIURIA, UROGENITAL TRACT AND KIDNEY INFECTIONS FROM URINARY PELLET SAMPLES

Номер: US20130178377A1
Автор: Fouts Derrick, Nelson Karen, Pieper Rembert, Suh Moojin
Принадлежит: J. Craig Venter Institute

Described herein are highly accurate metaproteomic based methods for diagnosing urogenital and kidney infections, which are easy to perform and that also provide information regarding the extent of the infection, the causative agent(s) and the nature of the host response. 1. A method comprising the steps of:(a) preparing a urinary pellet from a urine sample or sample prepared from a uretheral catheter associated biofilm;(b) preparing a protein mixture from the urinary pellet; and(c) analyzing the protein mixture using a metaproteomic technology approach.2. The method of claim 1 , wherein step (a) is performed by centrifuging the sample to obtain an insoluble pellet and re-suspending the pellet in a buffered solution.3. The method of claim 1 , wherein step (b) is performed by subjecting the urinary pellet to appropriate conditions for lysing and solubilizing microbial and/or host cells and solubilizing microbial and/or host extracellular aggregates so that the majority of proteins present in the pellet are susceptible to proteolytic digestion.4. The method of claim 3 , wherein the solubilized proteins derived from the urinary pellet are contacted with and fully or partially digested by an endopeptidase.5. The method of claim 1 , wherein the metaproteomic analysis comprises the steps of analyzing the digested protein mixture using an appropriate MS or MS/MS system to generate mass spectral data and searching the MS data with a compilation of protein sequence databases derived from annotated microbial genomes that represent at least some of the microbial species and the colonized mammalian host organism in the mixture.6. The method of claim 5 , wherein the searching step is comprised of a computational comparison of peptide m/z values derived from the proteins in the database via in silico digestion with a given endopeptidase to experimentally observed peptide m/z values.7. A method for diagnosing a subject with a urogenital tract or kidney infection claim 5 , ...

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Номер записи: 64
25-07-2013 дата публикации

Enzyme detection

Номер: US20130189719A1
Автор: Mark Burnapp, Mark James Davis, Paul James Davis, Sandra Hemmington
Принадлежит: MOLOGIC LTD

An enzyme detection product ( 1 ) for detecting the presence of an enzyme in a sample. The product ( 1 ) comprises: a reaction zone ( 16 ) for receiving the sample; a visualization zone ( 10 ) for presenting a signal in response to the detection of the activity of the enzyme; and a membrane ( 11 ). The membrane ( 11 ) is interposable between the reaction zone ( 16 ) and the visualization zone ( 10 ) and prevents passage from the reaction zone ( 16 ) to the visualization zone ( 10 ) the components having a size greater than a threshold size. The reaction zone ( 16 ) comprises a reactant capable of reacting with the enzyme in order to generate a reaction product having a size less than a threshold size.

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Номер записи: 65
25-07-2013 дата публикации

Identification of pathogens in body fluids

Номер: US20130189728A1
Автор: Weller Ulrich
Принадлежит: Bruker Daltonik GmbH

Identification of infectious pathogens, particularly viruses, bacteria and other microorganisms is effected with a method whereby pathogens of acute infections can be identified, without first culturing them in external nutrient media, by mass spectrometric measurement of their protein profiles obtained from pathogens directly precipitated from body fluid into pellets by centrifuging. With this method, pathogens which cause acute infections can be identified in less than one hour. 113-. (canceled)14. A method for the mass spectrometric identification of pathogens in a body fluid , the method comprising the steps of:a) culturing the pathogens in the body fluid by incubation;b) precipitating by centrifuging the pathogens from the body fluid into a pellet;c) subjecting the pathogens to a mass spectrometric analysis of their proteins; andd) identifying the pathogens by comparison of protein mass spectra with reference protein mass spectra.15. The method according to claim 14 , wherein claim 14 , in step b) claim 14 , the pathogens in the pellet are smeared directly onto a mass spectrometric sample support plate and sprinkled with matrix solution for ionization of proteins thereof by matrix-assisted laser desorption.16. The method according to claim 14 , wherein claim 14 , in step b) claim 14 , the proteins are extracted from the pathogens while the pellet with the pathogens still rests in a centrifuge tube and an extraction fluid is subjected to mass spectrometric measurement of the proteins.17. The method according to claim 16 , wherein claim 16 , for extraction of the proteins claim 16 , the pathogens of the pellet are decomposed by chemical claim 16 , physico-chemical or physical means.18. The method according to claim 16 , wherein the extraction fluid is subjected to mass spectrometric analysis using electrospray ionization.19. The method according to claim 17 , wherein the extraction fluid is subjected to mass spectrometric analysis using electrospray ionization.20 ...

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Номер записи: 66
01-08-2013 дата публикации

DEVICE FOR CELL CULTURE AND ANALYSIS

Номер: US20130196315A1
Автор: Chilosi Marco, Krampera Mauro, Ricciardi Mario
Принадлежит: UNIVERSITA' DEGLI STUDI DI VERONA

The present invention relates to a device for cell culture and analysis, comprising a first containing element, a second containing element sealingly couplable to one another to define a culture chamber and a flexible film housable in said culture chamber and available alternatively on one of said first element or second element. The flexible film can be made of a transparent material selected from the group consisting of polycarbonate, polystyrene and polyethylene. A method for cell analysis is also provided. 1123411423. A device () for cell culture and analysis , comprising a first containing element () , a second containing element () sealingly couplable to one another to define a culture chamber () and a flexible film () housable in said culture chamber () and available alternatively on one of said first element () or second element ().211. The device according to claim 1 , wherein said flexible film () is made of a material selected from the group consisting of polycarbonate claim 1 , polystyrene and polyethylene.323. The device according to or claim 1 , wherein said first containing element () and said second containing element () are made of transparent material.4. The device according to claim 3 , wherein said transparent material is selected from the group consisting of polystyrene claim 3 , polycarbonate and glass.525655. The device according to claim 1 , wherein said first containing element () comprises a substantially flat bottom wall () integral with side walls () which extend along peripheral edges of said bottom wall () and are transversal to said bottom wall ();{'b': 3', '13', '14', '6, 'said second element () comprising a substantially flat wall provided with peripheral edges () adapted to sealingly couple with corresponding peripheral edges () of said side walls ().'}623984810. The device according to claim 1 , wherein at least one of said first containing element () and said second containing element () is provided with a tubular appendix () ...

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Номер записи: 67
01-08-2013 дата публикации

In Vitro Tumor Metastasis Model

Номер: US20130196349A1
Автор: Martin George A., Noll Lee A., Pfund William P.
Принадлежит:

This invention provides a system and methods for modeling tumor metastasis in vitro where primary tumor tissue is cultivated in an orientation and an environment such that the natural composition, three-dimensional organization, and environmental conditions of the tumor can be adjusted. The invention further provides mechanism for inducing tumors to undergo metastatic processes resulting in production of tumor progenitor or stem cells that can be collected, characterized, or used to induce tumors in normal tissue constructs in vitro. 1. A combination of a bioreactor and one or more tumor cells in which the system parameters are such that the tumor cells maintain their normal metastatic potential.2. The combination of claim 1 , wherein the bioreactor comprises a cell-supporting but cell-permeable matrix separating at least two fluid chambers with fluid flowing therethrough and at least one gas chamber connected to each of the fluid chambers.3. The combination of claim 1 , wherein said one or more tumor cells have been introduced into one of the at least two fluid chambers containing suitable nutrient medium and gas sufficient to sustain tumor growth.4. The combination of claim 1 , wherein the normal metastatic potential comprises the ability to produce metastatic cells.5. The combination of claim 4 , wherein the metastatic cells include cells having characteristics of circulating tumor cells and circulating tumor progenitor cells.6. The combination of claim 5 , wherein the characteristics of circulating tumor cells and circulating tumor progenitor cells include the presence of one or more of the following biomarkers: EpCAM claim 5 , CK5 claim 5 , CK7 claim 5 , CK18 claim 5 , CK19 claim 5 , Cd44v6 claim 5 , EphB4 claim 5 , FAP (seprase) claim 5 , IGF-1R claim 5 , BCL2 claim 5 , HER2 claim 5 , CA19-9 claim 5 , CEA claim 5 , CD133 claim 5 , MUC1 claim 5 , N-cadherin claim 5 , Survivin and PTEN.7. A method of generating metastatic tumor cells in vitro comprising:(a) ...

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Номер записи: 68
01-08-2013 дата публикации

RAPID ANTIBIOTIC SUSCEPTIBILITY TESTING SYSTEM BASED ON BACTERIAL IMMOBILIZATION USING GELLING AGENT, ANTIBIOTIC DIFFUSION AND TRACKING OF SINGLE BACTERIAL CELLS

Номер: US20130196364A1
Автор: Choi Jung Il, Jung Yong-Gyun, Kwon Sunghoon, Na Hun Jong
Принадлежит:

A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results. 1. A testing method comprising:providing a mixture solution of a gelling agent and a microbe to a gelling device;solidifying the mixture solution to form a solid thin film in which the microbe is immobilized;supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; andimaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results.2. The testing method according to claim 1 , wherein the microbe is bacteria.3. The testing method according to claim 1 , wherein the density of the microbe in the mixture solution is from 10to 10cells/ml.4. The testing method according to claim 1 , wherein the thin film has a thickness of 1 μm to 5 mm.5. The testing method according to claim 1 , wherein the gelling device has at least one receiving portion having an internal space where a liquid medium containing the gelling agent is retained.6. The testing method according to claim 5 , wherein the receiving portion takes the form of a microwell claim 5 , a narrow flat recess claim 5 , a microfluidic channel claim 5 , or a combination thereof.7. The testing method according to claim 1 , wherein the gelling agent is selected from the group consisting of agar claim 1 , agarose claim 1 , ...

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Номер записи: 69
01-08-2013 дата публикации

Method and Culture Medium for Enhanced Detection of Mycobacterium

Номер: US20130196366A1
Автор: Barton Leticia, Deol Parampeol, Lovern Douglas, Portilla Yoany
Принадлежит: bioMerieux, Inc.

The present invention relates to an improved culture medium and method for the enhanced growth and detection of growth. The invention further relates to an improved mycobacterial reagent system or kit that can be used for the enhanced growth and detection of 111.-. (canceled)12. A method for the diagnosis of infection caused by a mycobacterium species , comprising the steps of: (a) providing a culture medium; (b) adding a nutrient supplement to said culture medium , said nutrient supplement additive comprising α-ketoglutarate; (c) adding a sample for which the presence or absence of said mycobacterium species is to be determined; and (d) analyzing said culture for the presence of said mycobacterium species , wherein a finding of the presence of said mycobacterium species indicates a positive diagnosis for said infection.13. The method of claim 12 , wherein said culture medium and nutrient supplement reduce the time to detection of mycobacteria growth by at least about 2 days.14. The method of claim 12 , wherein said culture medium and nutrient supplement reduce the lag phase of mycobacteria growth by at least about 1 day.15. The method of claim 12 , wherein said culture medium claim 12 , nutrient supplement and sample are subjected to conditions suitable for growth of said mycobacteria prior to step (d).16. The culture medium of claim 12 , wherein said culture medium is at a pH of between about 5.5 to about 7.5.17. The method of claim 12 , wherein said sample is selected from the group consisting of blood claim 12 , serum claim 12 , plasma claim 12 , blood fractions claim 12 , saliva claim 12 , sputum claim 12 , aspirates claim 12 , and swab samples.18. The method of claim 12 , wherein said culture medium further comprises an antimicrobial supplement claim 12 , and wherein said antimicrobial supplement is added prior to the addition of said sample in step (c).19. The method of claim 18 , wherein said antimicrobial supplement is suspended using said nutrient ...

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Номер записи: 70
08-08-2013 дата публикации

INDICATORS FOR DETECTING THE PRESENCE OF METABOLIC BYPRODUCTS FROM MICROORGANISMS

Номер: US20130202531A1
Автор: Booher Jon, Gorski Joel R., Sabnis Ram
Принадлежит:

Polymeric indicator films and pH indicating wraps are provided for visually monitoring, detecting, and/or determining the presence of metabolic byproducts from harmful or potentially harmful microorganisms. Also provided are methods of use and preparation of the polymeric indicator films. 1. A polymeric indicator film comprising a transparent polymer layer or layers and a plurality of pH indicating moieties , wherein the pH indicating moieties are entrapped within the polymer layer or between two or more of the polymer layers and further wherein the pH indicating moieties retain the transparency of the polymer film at neutral pH but which impart color to at least a portion of the film when exposed to an acidic pH.2. A polymeric indicator film comprising a polymer and a plurality of pH indicating moieties selected from heptamethoxy red and hexamethoxy red or a combination thereof , wherein the pH indicating moieties are entrapped within the polymer.3. A polymeric indicator film comprising:a) a first layer comprising one or more hydrophilic, hydronium ion penetrating layers;b) a second layer comprising one or more hydrophobic, water impermeable layers; andc) a pH indicator layer placed between the first layer and the second layer.4. The polymeric indicator film of wherein the polymer is selected from the group consisting of polyethylene terephthalate claim 1 , poly(vinylidene fluoride) claim 1 , poly(vinyl chloride) claim 1 , poly(vinylidene chloride) claim 1 , polypropylene claim 1 , phenoxy resins claim 1 , butadiene/styrene copolymers claim 1 , butadiene/methylstyrene copolymers claim 1 , poly(meth)acrylates claim 1 , butadiene/acrylonitrile copolymers claim 1 , ethylene/propylene copolymers claim 1 , polybutadiene claim 1 , polyisoprene claim 1 , poly(oxy-2 claim 1 ,6-dimethyl-1 claim 1 ,4-phenylene) claim 1 , poly(oxycarbonyloxy-1 claim 1 ,4-phenyleneisopropylidene-1 claim 1 ,4-phenylene) claim 1 , acrylonitrile styrene copolymers claim 1 , acrylonitrile/methyl ...

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Номер записи: 71
15-08-2013 дата публикации

NUCLEIC ACID DETECTION AND RELATED COMPOSITIONS METHODS AND SYSTEMS

Номер: US20130210016A1
Автор: Bearinger Jane P., DUGAN Lawrence, SOUZA Brian E.
Принадлежит: Lawrence Livermore National Security, LLC

Provided herein are methods and systems for loop-mediated isothermal amplification of target polynucleotides on a sample without sample preparation. Methods and systems herein described also allow detection of cells and in particular bacterial cells on an untreated sample comprising the cells, and allow in some embodiments specific detection of bacterial cells such as 1. A method to detect a target polynucleotide in an untreated sample , the method comprising performing loop-mediated isothermal amplification on the untreated sample with primers specific for the target polynucleotide; and detecting amplification of the target polynucleotide following the performing.2. The method of claim 1 , wherein the target polynucleotide is comprised in the untreated sample at a concentration lower than about 10 fg.3. The method of claim 1 , further comprising performing an additional assay to detect the target polynucleotide.4. The method of claim 1 , wherein the untreated sample further includes polynucleotides other than the target polynucleotide.5. The method of claim 1 , wherein the loop mediated isothermal amplification is performed in presence of a lytic enzyme or other reagents suitable to increase availability of the target polynucleotide.6. The method of claim 1 , wherein the target polynucleotide is comprised within a cell.7. The method of claim 6 , wherein the cell is a cellular microorganism.8Bacillus anthracis.. The method of claim 7 , wherein the cellular microorganism is9. The method of claim 6 , wherein the target polynucleotide is specific for the cell.10. A system for detection of a target polynucleotide in an untreated sample claim 6 , the system comprising primers specific for the target polynucleotide and reagents for performing loop-mediated isothermal amplification for simultaneous combined or sequential use in detecting target polynucleotide in an untreated sample.11. The system of claim 6 , wherein the primers and the reagents are for simultaneous claim ...

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Номер записи: 72
15-08-2013 дата публикации

METHOD OF DETECTING A BIOLOGICAL ACTIVITY

Номер: US20130210048A1
Автор: Chandrapati Sailaja, Engel Brian J., Pederson Jeffrey C., Webb Heather M.
Принадлежит: 3M INNOVATIVE PROPERTIES COMPANY

A method of detecting a biological activity is provided. The method includes contacting; in a liquid medium selected to facilitate a predetermined biological activity; a sample, an indicator reagent, and a substrate that receives and concentrates a biological derivative of the indicator reagent. The method further includes observing a portion of the substrate to detect the biological derivative. The method can be used to detect the presence or absence of a target cell. 19-. (canceled)10. A method of detecting a biological activity , comprising: a housing comprising a first chamber and a second chamber;', 'a container containing a first aqueous liquid, the container disposed in the first chamber, wherein at least a portion of the container is frangible, the first aqueous liquid comprising an indicator system comprising an indicator reagent that can be converted by a predetermined biological activity to a biological derivative;', 'a source of the predetermined biological activity disposed in the second chamber; and', 'a substrate that receives and concentrates the indicator reagent or the biological derivative from the aqueous liquid, the substrate disposed in the housing;, 'providing a biological sterilization indicator comprising;'}bringing the first aqueous liquid into fluid communication with the substrate to form a second aqueous liquid in which a concentration of the indicator reagent is lower than the concentration of the indicator reagent in the first aqueous liquid; andobserving a portion of the substrate to detect a presence or absence of the biological derivative.11. The method of claim 10 , wherein bringing the first aqueous liquid into fluid communication with the substrate comprises fracturing at least a portion of the container.12. The method of claim 10 , wherein the housing of the biological sterilization indicator includes:a first portion, anda second portion adapted to be coupled to the first portion, the second portion being movable with respect to ...

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Номер записи: 73
15-08-2013 дата публикации

COMPOSITIONS AND ASSAYS FOR DETERMINING CELL VIABILITY

Номер: US20130210063A1
Автор: Hanson Bonnie Jean
Принадлежит: LIFE TECHNOLOGIES CORPORATION

Provided are compositions and methods useful in evaluation of cell health and metabolism, cell viability, proliferation, and the effects of compounds on these qualities. The assays provided are rapid, robust, nontoxic and suitable for use with high throughput devices.

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Номер записи: 74
15-08-2013 дата публикации

SUBSTRATE USED FOR CELL MIGRATION ASSAYS AND METHOD FOR CELL MIGRATION ASSAYS

Номер: US20130210068A1
Автор: Akai Tomonori, TAKASUGI Yuya, YOKOYAMA Naoki
Принадлежит: Dai Nippon Printing Co., Ltd.

This invention provides a means for cell migration assays by regulating the cells adhered to a substrate so that they migrate to a given region, which is a substrate used for cell culture comprising: a base material comprising a conductive region and an insulating region provided thereon and cell-adhesive regions and non-cell-adhesive regions provided in the conductive region and the insulating region, respectively, wherein a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the conductive region, and a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the insulating region. 1. A substrate used for cell culture , comprising a base material comprising a conductive region and an insulating region provided thereon , cell-adhesive regions and non-cell-adhesive regions provided in the conductive region , and non-cell-adhesive regions provided in the insulating region , wherein a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the conductive region.2. The substrate used for cell culture according to claim 1 , comprising a base material comprising a conductive region and an insulating region provided thereon and cell-adhesive regions and non-cell-adhesive regions provided in the conductive region and in the insulating region claim 1 , respectively claim 1 , wherein a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the conductive region and a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the insulating region.3. The substrate used for cell culture according to claim 1 , wherein the non-cell-adhesive region in the conductive region can be modified to become cell adhesive by applying an electric potential to the conductive region.4. The substrate used for cell culture according to claim 1 , wherein the cell-adhesive region is modified from a non-cell-adhesive hydrophilic membrane comprising an organic compound containing a carbon- ...

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Номер записи: 75
15-08-2013 дата публикации

Method to Generate Novel Bioactive Molecules

Номер: US20130211100A1
Автор: Charusanti Pep, Palsson Bernhard
Принадлежит:

The present invention describes a method to generate new chemical entities (NCEs) that have well-defined activities such as, but not limited to, anti-bacterial, antifungal and anthelmintic effects. The NCEs are generated through adaptive evolution of one microbe (the producer) against another organism or cell type (the target). The producer is made to compete against the target over time by co-culturing the two together and serially passing the producer organism until the producer adaptively evolves by synthesizing an NCE(s) that inhibits growth of or kills the target. The molecular structure of the chemical entity (or entities) is then clucidated using tools from genomics, molecular biology, computational biology, analytical chemistry, organic chemistry and related fields. 1. A method for identifying a bioactive compound in a culture comprising:(i) co-culturing two or more organisms, wherein at least one organism is a producer and at least one other organism is a target;(ii) detecting inhibition of growth of the target organism(s); and(iii) detecting the presence of one or more bioactive compounds in the co-culture, thereby identifying a bioactive compound.2. The method of claim 1 , further comprising isolating from the co-culture one or more producers after the detecting of (ii).3. The method of claim 1 , further comprising isolating from a producer at least one compound with bioactivity against the target(s) from the co-culture of (i).4. The method of claim 1 , further comprising repeating (i)-(iii) at least once with the isolated producer and the target.5. The method of claim 1 , further comprising identifying the bioactive compounds by chemical structure elucidation means.6. The method of claim 5 , wherein the chemical structure elucidation means is mass spectrometry (MS) or nuclear magnetic resonance spectroscopy (NMR).7Streptomyces clavuligerus.. The method of claim 1 , wherein the co-culture comprises8Staphylococcus aureus.. The method of claim 1 , wherein ...

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Номер записи: 76
22-08-2013 дата публикации

PNEUMOCOCCAL SEROTYPE 6D

Номер: US20130216576A1
Автор: Nahm Moon H., PARK In Ho
Принадлежит: UAB RESEARCH FOUNDATION

Disclosed is a new and emerging serotype of designated serotype 6D, and assays and monoclonal antibodies useful in identifying same. Also disclosed is a novel pneumococcal polysaccharide with the repeating unit→2) glucose 1 (1→3) glucose 2 (1→3) rhamnose (1→4) ribitol (5→phosphate. This new serotype may be included in pneumococcal vaccines. 1Streptococcus pneumoniae. An isolated bacterial strain designated 6D; said bacterial strain characterized as having a capsular polysaccharide having the repeating unit {→2) glucose 1 (1→3) glucose 2 (1→3) rhamnose (1→4) ribitol (5→phosphate}.2Streptococcus pneumoniae. A vaccine comprising a polysaccharide derived from an isolated bacterial strain designated 6D; said bacterium characterized as having a capsular polysaccharide having the repeating unit {→2) glucose 1 (1→3) glucose 2 (1→3) rhamnose (1→4) ribitol (5→phosphate}.3. A vaccine comprising a purified polysaccharide having the repeating unit {→2) glucose 1 (1→3) glucose 2 (1→3) rhamnose (1→4) ribitol (5→phosphate}.4Streptococcus pneumoniae. An isolated or purified antigen binding molecule that binds to 6D; wherein the antigen comprises a polysaccharide having the repeating unit {→2) glucose 1 (1→3) glucose 2 (1→3) rhamnose (1→4) ribitol (5→phosphate}.5. A composition comprising purified or isolated mono- or polyclonal antibodies that react with a purified or isolated polysaccharide having the repeating unit {→2) glucose 1 (1→3) glucose 2 (1→3) rhamnose (1→4) ribitol (5→phosphate}.6Streptococcus pneumoniae. A chemical , physical , or genetic test that differentiates for the bacterium 6D , wherein said bacterium is differentiated based on a capsular polysaccharide having the repeating unit {→2) glucose 1 (1→3) glucose 2 (1→3) rhamnose (1→4) ribitol (5→phosphate}. This application is a divisional of U.S. patent application Ser. No. 12/601,896, filed May 4, 2010, which is related to and claims the benefit of PCT Application No. U.S.08/064,951, filed May 28, 2008, and U.S. ...

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Номер записи: 77
22-08-2013 дата публикации

RAPID MICROBIAL DETECTION AND ANTIMICROBIAL SUSCEPTIBILITY TESTING

Номер: US20130217063A1
Автор: Buttry Daniel A., Goldberg David A., Howson David C., Metzger Steven W.
Принадлежит: Accelerate Diagnostics, Inc.

A method for the detection of microorganisms in a sample comprising contacting said sample with a biosensor concentration module, allowing microorganisms to grow for a first period of time and detecting growth of discrete microorganisms as an indication of the presence of said microorganisms. 1. A method , comprising:determining, by a computer-based system configured to analyze microorganism information and comprising a processor, a tangible memory, and an interface, a first value corresponding to a growth rate of a microorganism, based on information from a microorganism detection device, and in response to incubating the microorganism in a test condition;obtaining, by the computer-based system, a second value corresponding to a growth rate of a reference microorganism in response to incubating the reference microorganism in a reference condition, wherein the second value is at least one of a predetermined second value and a dynamically determined second value; andidentifying, by the computer-based system, a difference between the growth rate of the microorganism and the growth rate of the reference organism based on the first value being proportionally different than the reference value.2. The method of claim 1 , wherein the test condition comprises a concentration of an antimicrobial agent.3. The method of claim 2 , wherein the difference between the growth rate of the microorganism and the growth rate of the reference microorganism signifies a susceptibility of the microorganism to the antimicrobial agent.4. The method of claim 3 , wherein the susceptibility is determined within a timeframe associated with 1 to 10 doubling times of the microorganism.5. The method of claim 3 , wherein the susceptibility is determined within a timeframe associated with 1 to 4 doubling times of the microorganism.6. The method of claim 3 , wherein the susceptibility is determined within a timeframe associated with 1 to 2 doubling times of the microorganism.7. The method of claim 1 , ...

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Номер записи: 78
29-08-2013 дата публикации

METHOD FOR THE PRODUCTION OF 2-BUTANOL

Номер: US20130224728A1
Автор: Bramucci Michael G., Flint Dennis, JR. Edward S., MILLER, Nagarajan Vasantha, Sedkova Natalia, Singh Manjari, Van Dyk Tina K.
Принадлежит: BUTAMAX(TM) ADVANCED BIOFUELS LLC

A method for the production of 2-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product. 138-. (canceled)40. The recombinant microbial host cell of claim 39 , wherein the acetolactate synthase has an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 4 claim 39 , SEQ ID NO: 77 claim 39 , and SEQ ID NO: 79 based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10 claim 39 , GAP LENGTH PENALTY=0.1 claim 39 , and Gonnet 250 series of protein weight matrix.41. The recombinant microbial host cell of claim 39 , wherein the acetolactate decarboxylase has an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 2 claim 39 , SEQ ID NO: 81 claim 39 , and SEQ ID NO: 83 based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10 claim 39 , GAP LENGTH PENALTY=0.1 claim 39 , and Gonnet 250 series of protein weight matrix.42. The recombinant microbial host cell of claim 39 , wherein the butanediol dehydrogenase has an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 6 claim 39 , SEQ ID NO: 85 claim 39 , SEQ ID NO: 87 claim 39 , and SEQ ID NO: 89 based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10 claim 39 , GAP LENGTH PENALTY=0.1 claim 39 , and Gonnet 250 series of protein weight matrix.44. The recombinant microbial host cell of claim 39 , wherein the butanol dehydrogenase has an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 14 claim 39 , SEQ ID NO: 72 claim 39 , SEQ ID NO: 75 claim 39 , and SEQ ID NO: 91 based on ...

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Номер записи: 79
29-08-2013 дата публикации

METHOD FOR DETECTING MICROORGANISMS WITH A SPECIMEN

Номер: US20130224788A1
Автор: AISSA Jamal, Montagnier Luc
Принадлежит: NANECTIS BIOTECHNOLOGIES SA

A process for preparing reagents for a microorganism detection test comprising: a) centrifuging medium containing a microorganism; b) filtrating the supernatant from (a); c) preparing diluted samples of the filtrate from (b); d) treating the diluted samples from (c) with an E/M field; e) detecting signals emitted from (d) using a solenoid; f) selecting samples from (e) whose signal amplitude is ≧1.5 times greater than background noise emitted by water or that present a frequency displacement towards higher values; g) placing the diluted samples from (f) into an enclosures protecting against external electromagnetic fields; h) splitting a diluted sample from (g), volume by volume, into two tubes, T and T where tube T is protected from external electromagnetic field interferences, and reference tube T is also placed in a protective enclosure and subjected subsequently to the presence or contact of a sample suspected of containing a specific microorganism. 111-. (canceled)12. A process for preparing a reagent for use in a microorganism detection test , comprising:a) centrifuging a biological or artificial liquid medium containing a microorganism, thereby resulting in a supernatant and a pellet;b) filtering the supernatant obtained in step (a), thereby resulting in a filtrate;{'sup': '−15', 'c) preparing a series of tenfold dilutions of the filtrate obtained in step (b), down to a filtrate dilution of a factor of 10, thereby resulting in diluted samples;'}d) submitting said diluted samples obtained in step (c) to an electrical, magnetic, and/or electromagnetic exciting field, thereby resulting in electrical signals;e) detecting and analyzing the electrical signals of step (d) using a solenoid and converting the electrical signals from analog form to digital form, and digitally recording said electrical signals;f) selecting diluted samples from which the electrical signals detected and analyzed in step (e) have amplitudes that are at least 1.5 times greater than ...

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Номер записи: 80
29-08-2013 дата публикации

STRUCTURED POLYDIORGANOSILOXANE POLYAMIDE CONTAINING DEVICES AND METHODS

Номер: US20130224844A1
Автор: Hulteen John C., Johnston Raymond P., Mazurek Mieczyslaw H., Richmond Mark R., Sherman Audrey A.
Принадлежит: 3M INNOVATIVE PROPERTIES COMPANY

Devices including a polydiorganosiloxane polyamide containing material having a microstructured surface are disclosed herein. Such devices can optionally include a flex circuit attached to the microstructured surface and can be useful, for example, in fluid handling applications. 1. A testing device comprising:a flex circuit; anda structured material attached to the flex circuit, wherein the structured material comprises one or more polydiorganosiloxane polyamides.2. The testing device of claim 1 , wherein the structured material is a microstructured material.3. The testing device of claim 2 , wherein the surface of the structured material that is attached to the flex circuit is a microstructured surface.4. The testing device of claim 1 , wherein the structured material is adhered directly to at least a portion of the flex circuit.5. The testing device of claim 4 , wherein the structured material is adhered directly to at least a portion of the flex circuit without additional adhesive.6. The testing device of claim 1 , wherein the flex circuit comprises a polyester substrate.7. The testing device of claim 6 , wherein the flex circuit comprises a polyethylene terephthalate substrate.8. The testing device of claim 1 , further comprising a source of potential.9. The testing device of claim 1 , wherein the testing device comprises an environmental testing device.10. The testing device of claim 1 , wherein the testing device tests the purity of water claim 1 , the presence of bacteria in food claim 1 , the presence of pathogens in food claim 1 , or a combination thereof.11. A method of making a testing device claim 1 , the method comprising:providing a structured material comprising one or more polydiorganosiloxane polyamides; andattaching the structured material to a flex circuit.12. The method of claim 11 , wherein the flex circuit is a film-based flex circuit.13. The method of claim 11 , wherein the flex circuit comprises a polyester substrate.14. The method of claim ...

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Номер записи: 81
05-09-2013 дата публикации

Microorganism number measurement device

Номер: US20130229168A1
Автор: Toshiaki Takeshita
Принадлежит: Panasonic Corp

A microorganism number-measuring apparatus includes: a container holder for holding container having an opening in the upper surface of the container, with the opening being positioned upward; and a rotary driver for rotating a liquid accommodated in container held by the holder, about the rotary axis in the up-and-down direction. Moreover, the apparatus includes: an electrode inserting part for inserting measurement chip to a position from above container held by the holder, via the opening, with the position being closer to the container's inner surface than to the container's center axis and being away from the container's inner surface with a predetermined distance; and a measurement unit for measuring microorganisms using measurement electrode of measurement chip inserted into container by the electrode inserting part. The electrode inserting part holds measurement chip, in a state of measurement electrode facing the container's inner-surface.

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Номер записи: 82
12-09-2013 дата публикации

PROCESS OF DIRECTLY DETECTING AND IDENTIFYING A MICROORGANISM IN A BIOLOGICAL SAMPLE DILUTED IN AN ENRICHMENT BROTH

Номер: US20130236883A1
Автор: ATRACHE Vincent, Colin Bruno, LAFAY Aurelie, MAKROUF Bouchra, Montes Pascal, MOSTICONE David, Raymond Jean Claude, Thierry Sofia, Vimont Antoine
Принадлежит:

The present invention generally relates to the field of analysis for example biological analysis. More specifically, the present invention relates to a process of detecting at least one microorganism present in a sample placed in a closed container, said method comprising essentially the following steps: 1. A process of detecting at least one microorganism present in a sample placed in a closed container , said method comprising essentially the following steps:a) Place said sample in contact in the container with at least one culture medium and a support capable of capturing the microorganism(s) to be detected,b) Close the container,c) Place the container under conditions capable of allowing the growth of the microorganism(s)d) Detect, inside said closed container, using detection means, the presence of the microorganism(s) fixed onto the capture support.2. The process according to claim 1 , in which a revealing system capable of allowing the detection is placed in contact in the container during step a).3. The process according to claim 1 , including an intermediate step c′) consisting in transferring all or part of the mixture constituted by said sample claim 1 , the culture medium claim 1 , the support capable of capturing the microorganism(s) to be detected and potentially of the revealing system claim 1 , from the container claim 1 , in this case called the main container claim 1 , to at least one second container called the secondary container.4. The process according to claim 1 , including a supplementary step e) consisting in confirming the detection of the microorganism(s) detected.5. The process according to claim 4 , wherein the confirming step e) is accomplished using a detection means which is identical or different from the detection means used for the detection step.6. The detection process according to claim 1 , wherein the detection means is taken from the group comprising: electrical detection means claim 1 , in particular electrochemical detection ...

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Номер записи: 83
19-09-2013 дата публикации

Methods and means for characterizing antibiotic resistance in microorganisms

Номер: US20130244230A1
Автор: Alexander F. Van Belkum, Gero P. Hooff, Jeroen van Kampen, Theo M. Luider, Wilhelm Goessens
Принадлежит: ERASMUS UNIVERSITY MEDICAL CENTER

The present invention relates to a method for characterizing the antibiotic resistance of a microorganism, said method comprising the steps of (a) providing a reference mass spectrum of an antimicrobial compound, its enzymatic modification product, its molecular target, or of a substrate compound of a its modifying enzyme; (b) exposing a microorganism, a cell lysate thereof, or a growth medium supernatant thereof, to said antimicrobial compound or said substrate compound in aqueous liquid to thereby provide an exposed sample; (c) acquiring a mass spectrum of the exposed sample; (d) comparing the mass spectrum acquired in step c) with the reference mass spectrum of step (a), and (e) determining from said comparison whether modification of said antimicrobial compound, its modification product or its molecular target or of said substrate has occurred following said exposure, and establishing that said microorganism is potentially resistant to said antimicrobial compound when said modification is observed.

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Номер записи: 84
19-09-2013 дата публикации

STRAINS OF SACCHAROMYCES CEREVISIAE

Номер: US20130244247A1
Автор: Albers Eva, Koppram Rakesh, Olsson Lisbeth
Принадлежит: SCANDINAVIAN TECHNOLOGY GROUP AB

A method for producing a strain of with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of obtained by the method according to the invention are described. 1Saccharomyces cerevisiae. A method for producing a strain of with introduced genes coding for xylose reductase , xylitol dehydrogenase and xylulokinase and with improved ethanol production , improved xylose conversion and reduced xylitol production , comprising the steps of:{'i': 'Saccharomyces cerevisiae', 'a) culturing cells of with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30° C.-35° C., and an airflow of 0.01-0.06 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means,'}b) and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor.2. The method according to claim 1 , wherein the medium of step a) comprises 15-25 g/l xylose claim 1 , preferably about 20 g/l.3. The method according to claim 1 , wherein the method further comprises the step of:c) subjecting the cells from step b) to increasing ...

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Номер записи: 85
19-09-2013 дата публикации

PAPER STRIP FOR DETERMINING MINIMUM INHIBITORY CONCENTRATIONS OF ANTIBIOTICS

Номер: US20130244316A1
Автор: Brocco Silvio
Принадлежит: Liofilchem S.R.L.

An article for determining a minimum inhibitory concentration of antibiotics. The article has a strip of paper in which the strip of paper is permeable to air so as to prevent air bubbles from forming at a point of contact with a microbial culture medium. The strip of paper has a predetermined concentration gradient of an antibacterial agent graded on a scale of fifteen dilution units. The antibacterial agent on the strip of paper is releasable slowly from the strip of paper. The scale of fifteen dilution units can he expressed on a color scale and can be expressed in μg/mL. The strip of paper is impregnated with the antibacterial agent. 1. An article for determining a minimum inhibitory concentration of antibiotics , the article comprising:a strip of paper in which the strip of paper is permeable to air so as to prevent air bubbles from forming at a point of contact with a microbial culture medium, said strip of paper having a predetermined concentration gradient of an antibacterial agent graded on a scale of fifteen dilution intervals.2. The article of claim 1 , the antibacterial agent on said strip of paper being releasable slowly from said strip of paper.3. The article of further comprising:a silica gel container receiving said strip of paper therein so as to protect said strip of paper from moisture.4. The article of claim 1 , said scale of fifteen dilution intervals being on a color scale.5. The article of claim 4 , said color scale expressed in μg/mL.6. The article of claim 1 , said scale of fifteen dilution intervals being expressed in μg/mL.7. The article of claim 1 , said strip of paper being impregnated with said antibacterial agent. The present application is a continuation-in-part of U.S. application Ser. N. 13/392,308, filed on Feb. 24, 2012, and entitled “Paper Strip for Determining Minimum Inhibitory Concentrations of Antibiotics”, presently pending.Not applicable.Not applicable.Not applicable.1. Field of the InventionThe present invention relates to ...

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Номер записи: 86
19-09-2013 дата публикации

Flow Cytometer Sorter

Номер: US20130244317A1
Автор: Bay Charles S., Degeal Jeffrey W., Malachowski George C., Purcell Paul Barclay, Stanton Edward A.
Принадлежит:

Disclosed are computer-implemented methods of sorting particles from a particle stream in a flow cytometer. The methods include: calculating sort decision making parameters using the raw event data values from a flow cytometer and a sort logic; performing sort logic computations using the sort logic definition and the sort decision making parameters to generate sort decisions; converting the sort decisions into sort commands; and sending the one or more sort commands to the flow cytometer. Sort logic computations may include algorithmically using conditional branching logic, and may include sort logic equations having mathematical functions characterizing one or more regions of interest in multidimensional data space. Such mathematical functions may be determined based on one or more parameters provided by a user. Also disclosed are corresponding systems having a flow cytometer and a computer. 1. A system for sorting particles from a particle stream , comprising:(a) a flow cytometer; and (i) an input that receives a sort logic definition;', '(ii) a sort parameters module that calculates one or more sort decision making parameters based on raw event data values received from the flow cytometer and the sort logic definition; and', '(iii) a sort decision module wherein the sort decision module determines one or more sort decisions by performing sort logic computations using the sort logic definition and the one or more sort decision making parameters, and wherein the sort decision module converts the one or more sort decisions into one or more sort commands and sends the one or more sort commands to the flow cytometer., '(b) a computer coupled to the flow cytometer, wherein the computer comprises2. The system of claim 1 , wherein the computer further comprises an event status and storage module and wherein the event status and storage module stores sort decisions and corresponding sort status and verification data.3. The system of claim 1 , wherein the sort decision ...

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Номер записи: 87
26-09-2013 дата публикации

Biologic Machines for the Detection of Biomolecules

Номер: US20130252231A1
Автор: Albrecht Jeff, Conrad Andrew J.
Принадлежит: LABORATORY CORPORATION OF AMERICA HOLDINGS

Disclosed are methods, devices and systems for the isolation and detection of biomolecules from a sample. The embodiments, detection of such biomolecules provides for detection of microorganisms. For example, disclosed are methods, devices and systems that use bacteriophage-based amplification of the signal in detection of bacteria and other microorganisms. The devices, systems and methods of the invention may allow for the detection of certain biomolecules peptides and ions in real time using minute amounts of sample. 1. A method for detecting a microorganism comprising the steps of:infecting the isolated microorganism with an infectious agent that can reproduce in the microorganism so as to produce progeny infectious agents;allowing the infected microorganism to release the progeny infectious agents; anddetecting the progeny infectious agents, wherein detection of the progeny infectious agents indicates that the microorganism is present in the sample.2. The method of wherein the progeny infectious agents comprise a component that allows for detection.3. The method of claim 2 , wherein the detectable component comprises a detectable biomolecule.4. The method of claim 1 , wherein the step of detecting the progeny infectious agents comprises immunodetection of the infectious agent.5. The method of claim 1 , further comprising separating the progeny infectious agents from the infected microorganism.6. The method of claim 5 , wherein the separating comprises a sized-based separation step.7. The method of claim 5 , wherein the separating comprises filtration of the progeny infectious agents through a pore claim 5 , wherein the pore size allows the progeny infectious agents to pass through the pore claim 5 , but does not allow the microorganism to pass through the pore.8. The method of claim 1 , further comprising the step of isolating the microorgansim from other components in the sample by binding of the microorganism to a binding agent.9. The method of claim 8 , ...

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Номер записи: 88
26-09-2013 дата публикации

Method and system for detection of microbial growth in a specimen container

Номер: US20130252271A1
Автор: Michael Ullery
Принадлежит: Biomerieux Inc

A method for determining whether microbial growth is occurring within a specimen container includes steps of incubating the specimen container and obtaining a series of measurement data points while the specimen container is incubated and storing the data points in a machine-readable memory. The series of measurement data points represent a growth curve of microbial growth within the specimen container. The methods determine a positive condition of microbial growth within the container from the measurement data points.

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Номер записи: 89
03-10-2013 дата публикации

Modular compositing screening protocols

Номер: US20130260411A1
Автор: Samadpour Mansour
Принадлежит:

Multiple samples are taken from multiple units of food product to be tested for pathogen or other microbes, with the samples being pooled for composite testing, individual testing being required only in the event that the pool indicates positive. 1. I claim the the methods stated in the above examples. The disclosure of U.S. Pat. No. 7,531,163 is incorporated by reference herein as background material. This-application provides additional inventive examples and methods in the same field of application. The examples and methods are as follows.is a flow chart that illustrates the first example. The steps for this example are as follows:(1) Collect twice as much sample (e.g. N=120 instead of N=60, or portions twice as big)a. Homogenize to uniform distributionb. Pool ½ portions of all samples (matrix+media)c. Incubate pool sample+individual samples until CFU>MDLd. Test poolReferring to , in step , sample is collected from individual units of product such as beef combos. More samples are preferably collected in this method because the samples will be split prior to enrichment, so the chances of having similar microbe populations in the split samples are better if a larger number of samples are taken. A larger number of samples is preferably, but not strictly necessary, and the method would work with the normal number of samples, with the caveat that it might be somewhat less accurate when microbe populations are very sparse. In step , the samples are homogenized to uniform distribution. In step , a portion of each sample is taken and placed into a poolstates that the portion is ½. This is a convenient portion, but other portions will also work. In step , the pooled samples are incubated to increase the population of microbes to detectable levels, as necessary. Preferably, the remaining portions of individual samples that were split off can also be incubated at this time so that they are ready for testing if need be. In step , the pool is tested. If the test shows ...

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Номер записи: 90
03-10-2013 дата публикации

Method and system for detecting microcystin from reflected light

Номер: US20130260416A1
Автор: Robert K. Vincent
Принадлежит: BOWLING BREEN STATE UNIVERSITY, BOWLING GREEN STATE UNIVERSITY

The present invention relates to a method of detecting microcystin in water from reflected light, and also includes devices for the measurement, calculation and transmission of data relating to that method.

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Номер записи: 91
10-10-2013 дата публикации

EFFERVESCENT COMPOSITIONS AND USES THEREOF

Номер: US20130266955A1
Автор: Beaurline Joseph M., Bjork Jason W., Liu Jie J., Mach Patrick A., Xia Wensheng
Принадлежит:

A latent effervescent body comprising a selective agent is disclosed. A method of using the latent effervescent body in a method to selectively enrich a target microorganism is also disclosed. The method comprises providing a sample, a culture medium, and the latent effervescent body. The method further comprises contacting the sample, the culture medium, and the latent effervescent body under conditions to facilitate growth of the target microorganism. The method further comprises releasing the selective agent from the latent effervescent body. Optionally, the method includes detecting a microorganism. 1. A method of enriching the growth of a target microorganism , comprising: wherein the latent effervescent body comprises an outer shell with a core composition disposed therein', 'wherein the core composition comprises two or more effervescent components and a selective agent that facilitates the growth of the target microorganism relative to growth of at least one other microorganism;, 'providing a culture medium, a sample that may include the target microorganism, and a latent effervescent body;'}contacting the sample, the latent effervescent body, and the culture medium under conditions to facilitate growth of the target microorganism; andreleasing the selective agent from the latent effervescent body.2. The method of claim 1 , wherein releasing the selective agent further comprises distributing the selective agent in the culture medium.3. The method of claim 2 , wherein distributing the selective agent further comprises distributing the selective agent via a mixing process resulting from gaseous effervescence.4. The method of claim 1 , wherein releasing the selective agent further comprises releasing the selective agent after an aqueous mixture comprising the sample and the culture medium are placed in fluidic contact with the latent effervescent body for a predetermined period of time.5. The method of claim 5 , wherein the predetermined time is less than or ...

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Номер записи: 92
17-10-2013 дата публикации

NEURAL CREST ENHANCERS AND METHODS FOR USE

Номер: US20130273596A1
Автор: Betancur Paola, Bronner Marianne, McKeown Sonja J., Sauka-Spengler Tatjana
Принадлежит:

DNA enhancer sequences are provided for use in constructs to identify early stage embryonic cells. The enhancer sequences can be used in parallel with short-hairpin RNA in a vector construct for endogenously regulated gene knockdowns. The disclosed enhancer sequences can be used to isolate a selected population of early stage embryonic cells. 1. An isolated nucleic acid sequence consisting of SEQ ID NO: 4.2. A nucleic acid vector for down-regulating gene expression comprising:a short-hairpin RNA sequence under transcriptional control of at least one enhancer sequence having the sequence as set forth in SEQ ID NO: 4.3. An expression vector comprising the sequence of SEQ ID NO: 4 and a nucleic acid sequence encoding a reporter protein.4. The expression vector of claim 3 , wherein the reporter protein is a fluorescent protein.5. The expression vector of claim 3 , wherein the expression vector is a viral vector.6. A method of isolating mammalian cells expressing FoxD3 claim 3 , the method comprising:introducing to a population of isolated mammalian cells an expression vector comprising the sequence of SEQ ID NO: 4 and a nucleic acid sequence encoding a reporter protein; andidentifying introduced cells that produce the reporter protein.7. The method of claim 6 , wherein the reporter protein is a fluorescent protein.8. The method of claim 6 , further comprising isolating the cells expressing the reporter protein.9. The method of claim 6 , wherein the isolating is performed by flow cytometry. This application is a divisional of U.S. patent application Ser. No. 12/645,431, filed Dec. 22, 2009 which claims the benefit of and priority to U.S. Provisional Application Ser. No. 61/203,334, filed Dec. 22, 2008, the entire contents of all of which are incorporated herein by reference.This invention was made with government support under Grant No. NS036485 awarded by the National Institutes of Health. The government has certain rights in the invention.The material in the text file ...

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Номер записи: 93
17-10-2013 дата публикации

PROCUREMENT EXTRACTION BAG

Номер: US20130273597A1
Автор: Huft Todd, Zajdowicz Jan
Принадлежит: ALLOSOURCE

There is disclosed a method of procuring allograft tissue. In an embodiment, the method includes inserting an allograft through an opening into a procurement extraction container, sealing the opening, adding extraction fluid through a second opening, agitating the fluid, collecting the fluid for detection of contamination, and sealing the second opening. There is disclosed a procurement extraction container. In one embodiment, the container includes a wall defining an interior to receive the allograft, an opening into the interior, sized to receive the allograft, a closure device for the opening, the closure device providing a hermetic seal, a second opening providing a second passageway into the interior, the second opening having a connector interface and a second closure device providing a hermetic seal so as to provide, with the first closure and the at least one wall, a sterile barrier. Other embodiments are also disclosed. 1. A method of procuring an allograft tissue , the method comprising:inserting an allograft tissue through a first opening into a procurement extraction container;sealing the first opening of the procurement extraction container;adding an extraction fluid from an extraction solution bottle through a second opening into the procurement extraction container;agitating the extraction fluid within the procurement extraction container;collecting the extraction fluid into the extraction solution bottle from the procurement extraction container for detection of contamination; andsealing the second opening of the procurement extraction container.2. The method of claim 1 , wherein the step of sealing the first opening includes forming a resealable seal with a zip-lock closure.3. The method of claim 1 , wherein the step of sealing the first opening includes forming a non-resealable seal with a glue bar so as to provide a one-time closure.4. The method of claim 1 , wherein the step of sealing the first opening includes forming a seal with a zip-lock ...

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Номер записи: 94
17-10-2013 дата публикации

Articles and method for detecting a target microorganism

Номер: US20130273598A1
Автор: Akio KITAHARA, Henry J. Lubrant, Patrick A. Mach, Takatoshi MORIYAMA
Принадлежит: 3M Innovative Properties Co

A method of detecting a target microorganism is disclosed. The method comprises providing a culture device with a selective culture medium and a detection article comprising a first indicator system. The selective culture medium facilitates the growth of an indicator microorganism. When an indicator microorganism is detected in a sample contacted with the culture medium, the detection article is contacted with the culture medium to detect the target microorganism.

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Номер записи: 95
17-10-2013 дата публикации

SENSOR CHIP AND MEASUREMENT METHOD USING THE SAME

Номер: US20130274126A1
Автор: Ishige Yu, Kamahori Masao
Принадлежит: Hitachi, Ltd.

Provided is a device that can detect cells or bacteria in units of a single cell or bacterium, and can further measure the amounts of activity of cells or bacteria or responses of the cells or bacteria to drugs in units of a single cell or bacterium. A plurality of partitioned regions each having about the same size as a cell or a bacterium is provided, and a plurality of types of electrical sensors and are arranged in each partitioned region. 1. A sensor chip comprising:a board;a plurality of partitioned regions provided on a surface of the board;a plurality of types of sensing portions provided in each of the plurality of partitioned regions; anda plurality of detection portions connected to the respective sensing portions.2. The sensor chip according to claim 1 , wherein each partitioned region has about the same size as a target to be measured.3. The sensor chip according to claim 2 , wherein the target to be measured is a cell or a bacterium.4. The sensor chip according to claim 2 , wherein one of the plurality of sensing portions is connected to a detection portion that detects the presence or absence of the target to be measured.5. The sensor chip according to claim 1 , wherein each partitioned region is a well provided on the board.6. The sensor chip according to claim 1 , wherein the plurality of sensing portions are arranged such that one of the sensing portions surrounds another sensing portion.7. The sensor chip according to claim 1 , wherein the plurality of sensing portions have different shapes.8. The sensor chip according to claim 1 , wherein each sensing portion includes one of noble metal claim 1 , carbon claim 1 , an oxide film claim 1 , a nitride film claim 1 , or an ion sensing film.9. The sensor chip according to claim 1 , wherein each detection portion is an alternating-current impedance measuring portion claim 1 , a direct-current redox current measuring portion claim 1 , or a potential measuring portion.10. The sensor chip according to claim ...

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Номер записи: 96
24-10-2013 дата публикации

CULTURE MEDIUM FOR MICROORGANISMS INCLUDING PARA-AMINOBENZOIC ACID AS A SELECTIVE AGENT

Номер: US20130280756A1
Автор: Roche Jean-Marc
Принадлежит: BIOMERIEUX

The field of the invention is the analysis of target microorganisms in a complex sample. The present invention more particularly relates to a culture medium for the detection of at least one target microorganism including: at least one natural or synthetic fermentation or enzymatic activity substrate, and at least one selective agent, which inhibits non-target microorganisms, constituted by para-amino benzoic acid, one of its derivatives or one of their salts, at a concentration of between 0.05 and 1 g/L. 1. A culture medium for the detection of at least one target microorganism including:at least one natural or synthetic fermentation or enzymatic activity substrate, andat least one selective agent, constituted by para-amino benzoic acid, one of its derivatives or one of their salts, at a concentration between 0.05 and 1 g/L.2Salmonella. The culture medium according to claim 1 , wherein the target microorganism is a genus bacterium.3. The culture medium according to claim 1 , wherein the non-target microorganisms are from the group notably including enterobacteria.4. The culture medium according to claim 1 , wherein the derivative of para-amino benzoic acid is from the group notably including: 4-aminobenzoic acid claim 1 , 4-(methylamino) benzoic acid claim 1 , 4-amino-2-chlorobenzoic acid claim 1 , 4-amino-3-nitro benzoic acid claim 1 , 4-aminosalicylic acid.5. The culture medium according to claim 1 , further comprising at least one chromogenic or fluorogenic enzyme substrate.6. The culture medium according to claim 1 , further including bile salts.7. The culture medium according to claim 1 , including sodium deoxycholate as the bile salt.8. The culture medium according to claim 1 , at least one other selective agent from the group including: Novobiocin claim 1 , Vancomycin claim 1 , Amphotericin claim 1 , Cefsulodin.9. The process for detecting a target microorganism claim 1 , in a mixed population of target and non-target cells claim 1 , comprising the steps ...

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Номер записи: 97
31-10-2013 дата публикации

DETECTION OF MICROORGANISMS INVOLVED IN METABOLISM OF ORGANIC ENVIRONMENTAL POLLUTANTS

Номер: US20130288294A1
Автор: Gazenko Sergey, Saul Michael T.
Принадлежит:

A method and point of use kit to determine under field conditions the presence and/or amount of a selected species capable of bioremediating organic environmental pollutants, such as petroleum hydrocarbons. 1PseudomonasPseudomonas. A method to enhance detection of selected microorganisms relative to other microorganisms in water and soil samples , the method comprisingplacing a water and/or soil sample into a selective nutrient media, andincubating the media to at least 20° C. but less than 37° C. for 24 h to 48 h,visually evaluating without microscopy the incubated media for presence of microbial colony formation, and{'i': Pseudomonas putida, Pseudomonas aeruginosa,', 'Pseudomonas cepacia, 'determining the presence and/or relative concentration of at least one of or microoganisms by formation of at least one colony on the media.'}2Pseudomonas. A method to enhance bioremediation of a water and/or soil sample potentially contaminated with organic environmental pollutants , the method comprising detecting the level of at least one microorganism in the water and/or soil sample in the absence of microscopy identification and in the absence of incubating the microorganisms at a controlled temperature.3Pseudomonas stutzeriPseudomonas fluorescens. The method of additionally determining at least one of or microorganisms.4PseudomonasPseudomonas putida, Pseudomonas aeruginosa, Pseudomonas cepacia, Pseudomonas stutzeri,Pseudomonas fluorescens.. The method of where the microorganisms are limited to and/or5. The method of where the selective growth media comprises a peptic digest of animal tissue; magnesium chloride; potassium sulfate; erythromycin; ampicillin sodium salt; triclosan; tetrazolium blue chloride; agar; and optionally glycerol.6. The method of where the selective growth media comprises a peptic digest of animal tissue 10 g/L-30 g/L; magnesium chloride 1 g/L-2 g/L; potassium sulfate 8 g/L-12 g/L; erythromycin 0.005 g/L-0.02 g/L; ampicillin sodium salt 0.005 g/L-0.02 ...

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Номер записи: 98
31-10-2013 дата публикации

Method and System for Detection and/or Characterization of a Biological Particle in a Sample

Номер: US20130288295A1
Автор: Clay Bradford, Hyman Jones, Thope Thurman, Walsh John
Принадлежит: bioMerieux, Inc.

The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample whereby the method may be accomplished non-invasively by utilizing a time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle, that may be present in the sample. 1. A method for detecting and characterizing a microorganism present in a sample , said method comprising: said sample with a growth composition , obtaining at least two time-dependent measurements directly from said sample and growth composition , wherein said at least two time-dependent measurements comprise directly measuring intrinsic fluorescence from said sample and growth composition and correlating said measurements to detect and characterize any biological particle present in said sample.2. The method according to where said sample comprises blood claim 1 , bodily fluids claim 1 , or an opaque substance and wherein said biological particle is a microorganism.3. The method according to wherein said sample is a clinical sample and said growth composition is a liquid culture media.4. The method according to wherein said sample is a non-clinical sample.5. The method according to wherein said biological particle is a microorganism and said microorganism is characterized into on one or more classification models selected from the group consisting of Gram Groups claim 1 , Clinical Gram Groups claim 1 , Therapeutic Groups claim 1 , Functional Groups claim 1 , and Natural Intrinsic Fluorescence Groups.6. The method according to wherein said classification model characterizes said microorganism into a Gram Group selected from the group consisting of Gram positive microorganisms that stain dark blue with Gram stain claim 5 , Gram negative microorganisms that stain red with Gram stain claim 5 , or ...

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Номер записи: 99
31-10-2013 дата публикации

METHODS OF GENERATING AND SCREENING FOR LYTIC CHIMERIC POLYPEPTIDES

Номер: US20130288926A1
Автор: BUCHBERGER Bernd, Molinaro Sonja, Scherzinger Anna
Принадлежит: HYGLOS INVEST GMBH

The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonisation and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention. 1. A method of screening for a lytic chimeric polypeptide comprising the steps of: (i) the lytic domain of a bacteriophage lysin,', '(ii) the lytic domain of a bacteriocin,', '(iii) the lytic domain of a bacterial autolysin; and', '(iv) a bacteriophage tail-associated protein having lytic activity;, '(a) providing one or more DNA sequences each encoding at least one cell binding domain (CBD) and one or more DNA sequences each encoding at least one enzymatic active domain (EAD) and optionally one or more DNA sequences each encoding at least one CBD and at least one EAD, wherein the EAD is selected from the group consisting of'}{'sup': 'st', '(b) amplifying a first (1) domain sequence selected from the domain sequences of (a) using a pair of primers designed to introduce different restriction sites at the 5′-end and at the 3′-end, and a tag labeling at the 5′-end or at the 3′-end;'}{'sup': 'nd', 'claim-text': [{'sup': st', 'st', 'st, '(i) in case of 5′-end labelling of the 1domain: at the 5′-end the same restriction site as at the 3′-end of the 1domain, and at the 3′-end a restriction site different from the restriction sites introduced into the 1domain sequence,'}, {'sup': st', 'st', 'st, '(ii) in case of 3′-end labelling of the 1domain: at the 3′-end the same restriction site as at the 5′-end of the 1domain, and at the 5′-end a restriction site ...

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Номер записи: 100