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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 26534. Отображено 100.
21-07-2020 дата публикации

Устройство для калибровки газоаналитического оборудования в полевых условиях

Номер: RU0000198626U1
Автор: Владислав Андреевич Кузнецов, Марина Владимировна Волкодаева
Принадлежит: федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский горный университет"

Полезная модель относится к области технического и метрологического обеспечения мониторинга атмосферного воздуха и может быть использована для контроля качества получаемых первичных данных с газоаналитического оборудования.Устройство для калибровки газоаналитического оборудования в полевых условиях, содержащее калибровочный пакет прямоугольной формы. Корпус выполнен в форме прямоугольного параллелепипеда, внутри которого установлена перегородка, которая образует два отсека, внутри каждого из отсеков так же установлены перегородки, в отсеках установлены не менее чем по два калибровочных пакета в каждом, на передней грани корпуса выполнено сквозное отверстие, в которое установлены внешний кран, который расположен с внешней стороны корпуса, и внутренний кран - с внутренней стороны корпуса, на кранах закреплены с возможностью съема вентили регулировки порционной подачи/отбора калибровочной газовой смеси, к внутреннему крану с обеих сторон подключены магистрали, которые установлены над перегородкой внутри отсеков, на магистрали у задней грани корпуса установлен переходник, в нижней части корпуса каждого калибровочного пакета установлен фитинг, который соединен с магистралью, на противоположной стороне, по диагонали от него, установлен фитинг с заглушкой, по периметру корпуса калибровочного пакета выполнены внутренний и внешний шов путем спаивания. 6 ил. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 198 626 U1 (51) МПК G01N 30/04 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ (52) СПК G01N 30/04 (2020.02) (21)(22) Заявка: 2020109526, 03.03.2020 (24) Дата начала отсчета срока действия патента: 21.07.2020 Приоритет(ы): (22) Дата подачи заявки: 03.03.2020 (45) Опубликовано: 21.07.2020 Бюл. № 21 U 1 1 9 8 6 2 6 R U (54) УСТРОЙСТВО ДЛЯ КАЛИБРОВКИ ГАЗОАНАЛИТИЧЕСКОГО ОБОРУДОВАНИЯ В ПОЛЕВЫХ УСЛОВИЯХ (57) Реферат: Полезная модель относится к области установлены внешний кран, который расположен технического и метрологического ...

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Номер записи: 1
03-05-2012 дата публикации

Sample preparation for gas analysis using inductive heating

Номер: US20120103062A1
Автор: Carl Chang, Gregor Hsiao
Принадлежит: Picarro Inc

Improved gas analysis for non-gaseous samples is provided by placing the sample in direct contact with an inductive heating element, followed by inductively heating the heating element to provide gas for analysis. Disposable sample vials including such a heating element can be employed, or a sample tube including an inductive heating element can be configured to mate to the input gas line of a gas analysis system.

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Номер записи: 2
14-06-2012 дата публикации

Sample Collection System And Method

Номер: US20120144897A1
Автор: Robert S. Johnson, Stephen Scheufele
Принадлежит: Horizon Technology Inc

An apparatus or method for removing water and concentrating an analyte in solution, wherein the concentrated analyte sample is delivered directly to a vial, such as an autosampler vial that is capable of use in a gas chromatography autosampler.

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Номер записи: 3
14-06-2012 дата публикации

Field flow fractionator with controllable cross flow along its length

Номер: US20120148460A1
Автор: Philip J. Wyatt
Принадлежит: Wyatt Technology LLC

A field flow fractionator to separate particles contained within an injected sample aliquot is described. As required, said fractionator may be used to capture, for subsequent removal, specific predefined classes of such particles. Based upon the cross flow or asymmetric flow field flow fractionators, the fractionator disclosed contains means to vary the applied transverse flows at a plurality of locations along the length of its separating channel. One embodiment utilizes a plurality of separated compartments, each lying below a distinct and corresponding membrane supporting permeable frit segment, are provided individual means to control the localized flow through the membrane section thereabove. A corresponding concentric compartment implementation achieves the same type of compartmentalized cross flow when integrated with a hollow fiber fractionator.

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Номер записи: 4
15-11-2012 дата публикации

Method for determining equivalent thermal conditions between liquid chromatography systems

Номер: US20120285223A1
Автор: Peyton C. Beals, Richard W. Andrews
Принадлежит: Waters Technologies Corp

Described is a method of transferring a chromatographic method between liquid chromatography (LC) systems. The method is based on a determination of an isoretention temperature at which two solutes co-elute. The method enables separations to be performed using different LC systems with reproducible and equivalent results. For example, the method allows for a chromatography method developed for HPLC to be more readily transferred to a UPLC system and for a chromatography method developed for a UPLC system to be more readily transferred to a HPLC system. The method addresses LC systems having column ovens of different design in which the internal column temperatures are not equal although the operating temperatures of the column ovens may be accurately controlled to equal values. The retention behavior and resolution of different LC systems is caused to be substantially the same so that equivalent separation results are obtained.

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Номер записи: 5
03-01-2013 дата публикации

Self-sealing sample compartment for a liquid chromatography system

Номер: US20130004388A1
Автор: Daniel J. Gagne, James E. Usowicz, Joshua A. Shreve
Принадлежит: Waters Technologies Corp

Described is a self-sealing thermal enclosure. In various embodiments, the self-sealing thermal enclosure includes an enclosure having a wall with an opening. The enclosure is configured to surround a temperature-controlled environment. The self-sealing thermal enclosure also includes a porous seal disposed adjacent to the wall at the opening. The porous seal is compressible and is fabricated from an open cell foam material. When the porous seal has absorbed a fluid such as a condensate, the temperature-controlled environment is sealed from an ambient environment such that the flow of air into or out of the enclosure is substantially reduced or eliminated.

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Номер записи: 6
21-03-2013 дата публикации

MATERIALS AND METHODS FOR CAPILLARY MICROEXTRACTION IN COMBINATION WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Номер: US20130071945A1
Автор: Malik Abdul, Segro Scott S.
Принадлежит: UNIVERSITY OF SOUTH FLORIDA

Germania-based sol-gel organic-inorganic hybrid coatings were prepared for on-line coupling of capillary microextraction with high-performance liquid chromatography. A germania-based sol-gel precursor, tetra-n-butoxygermane and a hydroxy-terminated triblock copolymer, poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide), were chemically anchored to the inner walls of a fused silica capillary (0.25 mm I.D.). Scanning electron microscopy images of the sol-gel germania triblock polymer coating were obtained to estimate the coating thickness. The analyte distribution constants between a sol-gel germania organic-inorganic hybrid coating and the samples (K) were determined. For a variety of analytes from different chemical classes, including polycyclic aromatic hydrocarbons (PAHs), ketones, alcohols, phenols, and amines, the Kvalues ranged from 1.8×10to 2.0×10. The sol-gel germania triblock polymer coatings survived exposure to high temperature solvent conditions (200° C.) with little change in extraction capabilities. Reproducibility of the method for preparation of the sol-gel germania triblock polymer coatings was also evaluated, and the capillary-to-capillary RSD values ranged from 5.3% to 6.5%. The use of higher flow rates in extraction was found to significantly reduce the time required (from 30-40 minutes to 10-15 minutes) to reach equilibrium between the sol-gel germania triblock polymer coating and the analytes in the sample solution. 1. A solid-phase microextraction material for preconcentration of trace analytes in a sample , wherein a surface of the solid-phase microextraction material is coated with surface-bonded sol-gel germania triblock polymer that forms the stationary phase for the microextraction of the analytes.4. The solid-phase microextraction material of claim 1 , wherein the sol-gel germania triblock polymer is made from a sol-gel precursor that is tetra-n-butoxygermane (TNBG).5. The solid-phase microextraction material of ...

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Номер записи: 7
18-04-2013 дата публикации

BLOOD COLLECTION MODULE FOR MEASURING ALCOHOL CONCENTRATION

Номер: US20130091934A1
Автор: PARK Ik Hyun, Park Kwang Hee
Принадлежит: ELECHEM CO., LTD.

A blood collection module includes a blood collection container that is coupled with a blood alcohol concentration detection device including an alcohol detection sensor having a detection probe that inhales an alcohol gas, and on one surface of which an inserting portion that is inserted by a detection probe of a blood alcohol concentration detection device is formed and on an outer surface of which blood inlet holes through which blood flows in are formed; and an absorption member that is provided in the blood collection container, to thus absorb examinee's blood that is introduced through the blood inlet holes, in which an alcohol gas generated from the blood absorbed by the absorption member is introduced into the alcohol detection sensor through the detection probe. 1. A blood collection module for measuring blood alcohol concentration , the blood collection module comprising:a blood collection container which includes an inserting portion against which a detection probe of a blood alcohol concentration detection device having an alcohol detection sensor and the detection probe is inserted, and blood inlet holes through which blood is introduced, andan absorption member that is provided in the blood collection container to thus absorb examinee's blood that is introduced through the blood inlet holes,wherein an alcohol gas generated from the blood absorbed by the absorption member is introduced into the alcohol detection sensor through the detection probe.2. The blood collection module according to claim 1 , wherein the blood collection container further comprises a container cover that covers an upper surface of the blood collection container claim 1 , wherein the inserting portion is formed on the container cover in the form of a plurality of slits or score lines.3. The blood collection module according to claim 1 , wherein a handle that is laterally extended from the container cover is provided for the container cover and a tear-off portion is formed in the ...

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Номер записи: 8
25-04-2013 дата публикации

BIOMARKER FOR RENAL FUNCTION IN PATIENTS WITH TYPE 2 DIABETES

Номер: US20130101578A1
Автор: Chia Kee Seng, Holthofer Harry, Ng Peng Keat Daniel, Tai Bee Choo
Принадлежит: NATIONAL UNIVERSITY OF SINGAPORE

The invention provides kits and methods for the diagnosis, prognosis, and treatment of reduced kidney function in patients, such as diabetic patients. The methods include a step of detecting one or more nephrin degradation products in a sample from the subject, such as a urine sample. 1. A method of detecting type 2 diabetes-related reduced renal function in a mammalian subject comprising detecting one or more nephrin degradation products in a urine sample from the subject , wherein the nephrin degradation product has an apparent molecular weight of about 25 kDa , 50 kDa , 60 kDa and/or 75 kDa , and detection of the nephrin degradation product(s) indicates the presence of type 2 diabetes-related reduced renal function in the subject.2. The method of claim 1 , wherein the subject is a human.3. The method of claim 1 , wherein the subject is normoalbuminuric.4. (canceled)5. The method of claim 1 , wherein the nephrin degradation products has an apparent molecular weight of about 25 kDa.6. The method of claim 1 , wherein the nephrin degradation products is detected using an antibody.7. The method of claim 6 , wherein the antibody is a monoclonal antibody.8. The method of claim 1 , wherein the mammal exhibits a decline in eGFR of at least 1.0 ml/min/1.73 m.9. The method of claim 1 , wherein the subject with type 2 diabetes has been diagnosed as diabetic for a year or less.10. The method of claim 1 , wherein the nephrin degradation products is detected by ELISA claim 1 , Western Blotting claim 1 , RIA claim 1 , HPLC claim 1 , SPR claim 1 , nucleic acid or protein aptamers claim 1 , SAT claim 1 , peptide sequencing claim 1 , and/or MS/MS.11. The method of claim 1 , wherein the nephrin degradation products is detected in a cell free fraction of the urine sample.12. The method of claim 1 , further comprising administering a suitable treatment for reduced renal function claim 1 , wherein the treatment is one or more antagonists of the renin-angiotensin system (RAS).13. A ...

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Номер записи: 9
16-05-2013 дата публикации

Clocked Blowing Away of a Contaminated Gas Cloud

Номер: US20130118230A1
Автор: Aichinger Karl, Dunzinger Bernhard, Straubinger Hans-Jurgen
Принадлежит: KRONES AG

The disclosure relates generally to a method for testing containers for foreign substances, wherein a standard gas is blown into a container to be tested, at least a part of the test gas escaping from the container is tested by a measuring device, and the part of the test gas remaining outside the measuring device is removed from the measuring area in a clocked manner, e.g. by blowing it away or sucking it off. 1. A method for testing containers for foreign substances , the method comprising that a standard gas is blown into a container to be tested , that at least a part of the test gas escaping from the container is tested by a measuring device , and that the part of the test gas remaining outside the measuring device is removed from the measuring area in a clocked manner.2. A method according to claim 1 , the method comprising that the part of the test gas remaining outside the measuring device is blown away from the measuring area in a clocked manner under a high pressure if the tested container (B) is no longer located in the measuring area (R).3. A method according to claim 1 , wherein the part of the test gas remaining outside the measuring device is blown away and/or sucked off from the measuring area in a clocked manner only if a contamination of the tested container was detected.4. A method according to claim 1 , wherein at least a part of the test gas escaping from the container is tested by a measuring device chromatographically.5. A method according to claim 1 , wherein the blown-away part of the test gas remaining outside the measuring device is conducted to an outlet and/or is sucked off.6. A method according to claim 1 , wherein the standard gas comprises air claim 1 , an inert gas claim 1 , a noble gas claim 1 , a noble gas mixture claim 1 , or a combination of the aforementioned gases.7. A method according to claim 1 , wherein the part of the test gas remaining outside the measuring device is blown away with ambient air or technically purified air/ ...

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Номер записи: 10
30-05-2013 дата публикации

Method and Arrangement for Gas Chromatographic Analysis of a Gas Sample

Номер: US20130133403A1
Автор: Gellert Udo, Probst Frank
Принадлежит: SIEMENS AKTIENGESELLSCHAFT

The invention relates to a gas sample to be analyzed, wherein said sample is guided by means of a carrier gas through a separator unit having a downstream thermal conductivity detector providing a chromatogram having peaks for different analytes as a measurement signal. When using a thermal conductivity detector having a heated gold thread coated with a parylene F, hydrogen is used as a carrier gas, and a peak for the analyte hydrogen sulfide is generated by differentiating the chromatogram at the location of said analyte. The invention permits unlimited use of hydrogen as a carrier gas, even if the analyte is oxygen. 15.-. (canceled)6. A method for gas chromatographic analysis of a gas sample , comprising:conveying the gas sample by a carrier gas comprising hydrogen through a separating device having a downstream thermal conductivity detector including an electrically heated gold filament coated with parylene F;delivering, from the thermal conductivity detector, a chromatogram having peaks for different analytes as a measurement signal; anddifferentiating the chromatogram at a position of an analyte of the different analytes to generate a peak for a hydrogen sulfide analyte.7. The method as claimed in claim 6 , wherein the measurement signal is differentiated by an RC component formed by connecting the thermal conductivity detector by at least one capacitor to an input of an evaluation device.8. The method as claimed in claim 6 , wherein an oxygen analyte is detected in the carrier gas hydrogen.9. The method as claimed in claim 7 , wherein an oxygen analyte is detected in the carrier gas hydrogen.10. An arrangement for gas chromatographic analysis of a gas sample claim 7 , comprising:a separating device having a downstream thermal conductivity detector including an electrically heated gold filament coated with parylene F, the gas sample being conveyed by a carrier gas through the separating device, the thermal conductivity detector delivering a chromatogram having ...

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Номер записи: 11
30-05-2013 дата публикации

PRESSURE SENSING AND FLOW CONTROL IN DIFFUSION-BONDED PLANAR DEVICES FOR FLUID CHROMATOGRAPHY

Номер: US20130133760A1
Автор: Bunner Bernard, Dourdeville Theodore A., Gerhardt Geoff C.
Принадлежит: WATERS TECHNOLOGIES CORPORATION

Flow through pressure sensors for use in fluid chromatography systems include a planar device formed from diffusion bonding of a plurality of metallic sheets and at least one sensing element. The planar device has a top surface, a bottom surface and a flow through channel. A diaphragm formed from a portion of one of the top or bottom surfaces is located adjacent to a sensing region of the flow through channel and is attached to the sensing element. The diaphragm is sized to deflect a distance in response to fluid pressure in the sensing region, which has an internal volume of less than about 25 microliters. The diaphragm and attached sensing element form a pressure sensor that measures strain or deflection of the diaphragm to calculate a pressure within the sensing region. 1. A flow through pressure sensor for use in a system for chromatographic separation , the flow through pressure sensor able to withstand pressures of at least about 40 megapascals and comprising:a planar device formed from a plurality of metallic parts attached by diffusion bonding, the planar device having a top surface, a bottom surface, and at least one flow through channel disposed between the top and bottom surfaces; anda sensing element located on a diaphragm formed from a portion of at least one of the top surface or bottom surface of the planar device, the diaphragm bounding one face of a first sensing region of the at least one flow through channel and being sized to deflect a distance in response to fluid pressure in the first sensing region, the first sensing region having an internal volume of about 25 microliters or less.2. The flow through pressure sensor according to claim 1 , wherein the sensing element measures mechanical strain of the diaphragm for use in calculating fluid pressure in the first sensing region of the at least one flow through channel.3. The flow through pressure sensor according to claim 1 , wherein the sensing element measures deflection of the diaphragm for use ...

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Номер записи: 12
30-05-2013 дата публикации

AIRCRAFT SCREENING DEVICE AND METHOD

Номер: US20130137183A1
Автор: Nacson Sabatino
Принадлежит: Teknoscan Systems, Inc.

A method of screening aircraft passengers and/or cargo comprising loading an aircraft with passengers, cargo or both, circulating air within the aircraft so that the air is in contact with the passengers and/or cargo, expelling some of the circulated air from the aircraft, diverting a portion of the air being expelled from the aircraft through a chemical filter configured to retained evidence of a target substance, and analyzing the chemical filter to detect the presence of a target substance within the aircraft. 1. A method of screening aircraft passengers or cargo comprising:loading an aircraft with passengers, cargo or both;circulating air within the aircraft so that the air is in contact with the passengers or cargo;expelling some of the circulated air from the aircraft;diverting a portion of the air being expelled from the aircraft through a chemical filter configured to retain evidence of a target substance; andanalyzing the chemical filter to detect the presence of the target substance within the aircraft.2. The method of wherein the method comprises loading the aircraft with passengers and cargo claim 1 , the cargo comprising an air freight container claim 1 , and the method further comprises:drawing a sample from the container using suction before the container is loaded into the aircraft;passing the sample through a chemical filter configured to retain evidence of a target substance in the sample; andanalyzing the chemical filter to detect the presence of the target substance within the container.3. The method of claim 2 , further comprising:drawing a sample from a passenger's clothing or hand luggage using suction prior to the passenger boarding the aircraft;passing the sample through a chemical filter configured to retain evidence of a target substance, andanalyzing the chemical filter to detect the presence of the target substance on the passenger's clothing or hand luggage.4. The method of wherein air is circulated by activating an onboard air ...

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Номер записи: 13
06-06-2013 дата публикации

VOLATILE ORGANIC COMPOUNDS FOR DETECTING CELL DYSPLASIA AND GENETIC ALTERATIONS ASSOCIATED WITH LUNG CANCER

Номер: US20130143247A1
Автор: Haick Hossam, Peled Nir
Принадлежит: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD.

The present invention provides methods of identifying a genetic abnormality such as mutation in EGFR or KRAS or ALK which is associated with the management of lung cancer or diagnosing, prognosing or monitoring the treatment of pre-cancerous conditions of the lung, such as bronchial dysplasia or atypical alveolar hyperplasia (AAH), through the detection of at least one volatile organic compound indicative of these states. 1. A method of identifying a genetic alteration selected from a mutation in EGFR , a mutation in KRAS , an ALK-ELM4 translocation and CMET amplification , wherein the genetic alteration is associated with lung cancer , the method comprising the steps of:a) obtaining a sample from a test subject;b) determining the level of at least one volatile organic compound in the test sample; andc) comparing the level of the at least one volatile organic compound from the test sample with the level of said at least one volatile organic compound in a negative control sample, whereby a significantly different level of said at least one volatile organic compound in the test sample as compared to the level of said compound in the negative control sample is indicative of the presence of said genetic alteration.2. The method according to claim 1 , wherein the at least one volatile organic compound is selected from the group consisting of 4-methyl-1-heptanol claim 1 , acetic acid octyl ester claim 1 , decane claim 1 , 3-methyl-decane claim 1 , octanal claim 1 , pentadecanenitrile claim 1 , and tetradecene; or selected from the group consisting of 4-methyl-1-heptanol claim 1 , 6-methyl-1-heptanol claim 1 , 2-ethyl-1-hexanol claim 1 , acetic acid octyl ester claim 1 , benzaldehyde claim 1 , decance claim 1 , 3-methyl-dodecance claim 1 , tetrahydrofuran claim 1 , isopropyl myristate claim 1 , octanal claim 1 , pentadecanenitrile claim 1 , 2 claim 1 ,2 claim 1 ,4-trimethyl-pentanenitrile claim 1 , 2 claim 1 ,2 claim 1 ,4-trimethyl-3-carboxyisopropyl-isobutyl ester ...

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Номер записи: 14
27-06-2013 дата публикации

Method of measuring the electroosmotic transport coefficient of a proton exchange membrane and device for implementing such a method

Номер: US20130166222A1
Автор: Arnaud Morin, Zhe Peng
Принадлежит: Commissariat a lEnergie Atomique et aux Energies Alternatives CEA

A method of determining the electroosmotic transport coefficient of a proton exchange membrane, the method including creating a stream of hydrated hydrogen on either side of the membrane which is permanently controlled so that the relative humidity is almost identical on each side of the membrane at any point, thereby making it possible to minimize any back diffusion into the membrane. Furthermore, the method includes estimating the back diffusion flux into the membrane from the rate of return to equilibrium of the relative humidity starting from the moment when the current is cut off.

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Номер записи: 15
04-07-2013 дата публикации

MORPHOLOGY ENGINEERING OF CONDUCTIVE METALLIC NANOPARTICLES CAPPED WITH AN ORGANIC COATING

Номер: US20130171733A1
Автор: GLIKSMAN Sadi, Haick Hossam, SEGEV-BAR Meital, SHUSTER Gregory
Принадлежит: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD.

The present invention is directed to a sensor having continuous and discontinuous regions of conductive metallic nanoparticles capped with an organic coating which enables the detection of volatile organic compounds and/or water vapor. 1. A sensor for detecting an analyte selected from a volatile organic compound , water vapor and combinations thereof , the sensor comprising continuous and discontinuous regions of conductive metallic nanoparticles capped with an organic coating , wherein the continuous and discontinuous regions differentially detect water vapor and volatile organic compounds.2. The sensor according to claim 1 , wherein the continuous regions exhibit a positive response upon exposure to volatile organic compounds and to water vapor claim 1 , and the discontinuous regions exhibit a positive response upon exposure to volatile organic compounds and a negative response upon exposure to water vapor.3. The sensor according to claim 1 , wherein the discontinuous regions comprise voids ranging in size from about 10 nm to about 500 nm.4. The sensor according to configured in a form selected from the group consisting of a capacitive sensor claim 1 , a resistive sensor claim 1 , a chemiresistive sensor claim 1 , an impedance sensor claim 1 , and a field effect transistor sensor.5. The sensor according to which is a chemiresistor comprising a film comprising continuous and discontinuous regions of conductive metallic nanoparticles capped with an organic coating formed on a substrate.6. The sensor according to claim 5 , wherein the substrate is a rigid substrate or a flexible substrate.7. The sensor according to claim 5 , wherein the substrate is selected from the group consisting of metals claim 5 , insulators claim 5 , semiconductors claim 5 , semimetals claim 5 , polymers claim 5 , and combinations thereof.8. The sensor according to claim 7 , wherein the substrate a polymer selected from the group consisting of polyimide claim 7 , polyamide claim 7 , polyimine ...

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Номер записи: 16
04-07-2013 дата публикации

REAGENT COMPOSITION FOR NUCLEIC ACID CHROMATOGRAPHY OR IMMUNOCHROMATOGRAPHY, METHOD FOR MEASUREMENT BY NUCLEIC ACID CHROMATOGRAPHY OR IMMUNOCHROMATOGRAPHY, AND KIT FOR MEASUREMENT BY NUCLEIC ACID CHROMATOGRAPHY OR IMMUNOCHROMATOGRAPHY

Номер: US20130171740A1
Автор: Sakakibara Yuhiro
Принадлежит: TANAKA KIKINZOKU KOGYO K.K.

A reagent composition for nucleic acid chromatography or immunochromatography which includes a water-soluble polymer having a weight average molecular weight of 8,000 or more, a salt of a divalent or trivalent metal, a nonionic surfactant, and an aprotic water-soluble organic compound. The reagent composition is capable of determining an analyte accurately and rapidly even when it has a low concentration in a measurement by nucleic acid chromatography or immunochromatography, by reducing the binding of components other than the analyte through a non-specific reaction and enhancing the dispersion capability of the analyte to improve the developability on a chromatography carrier and to promote a specific reaction. 1. A reagent composition for nucleic acid chromatography or immunochromatography comprising a water-soluble polymer having a weight average molecular weight of 8 ,000 or more , a salt of a divalent or trivalent metal , a nonionic surfactant , and an aprotic water-soluble organic compound.2. The reagent composition according to claim 1 , wherein the water-soluble polymer having a weight average molecular weight of 8 claim 1 ,000 or more is one or more kinds selected from the group consisting of polyalkylene glycols claim 1 , celluloses claim 1 , vinyl-based polymers claim 1 , amide-based polymers claim 1 , and a polyanion.3. The reagent composition according to claim 2 , wherein the polyanion is a polysaccharide anion.4. The reagent composition according to claim 3 , wherein the polysaccharide anion is one or more kinds selected from the group consisting of dextran sulfate claim 3 , heparan sulfate claim 3 , chondroitin sulfate claim 3 , dermatan sulfate claim 3 , keratan sulfate claim 3 , hyaluronic acid claim 3 , heparin claim 3 , and salts thereof.5. The reagent composition according to claim 1 , wherein the aprotic water-soluble organic compound is one or more kinds selected from the group consisting of sulfoxides and N claim 1 ,N′-dialkylamides.6. The ...

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Номер записи: 17
25-07-2013 дата публикации

Micro-scale passive vapor preconcentrator/injector

Номер: US20130186174A1
Автор: Edward T. Zellers, Jung Hwan Seo, Katsuo Kurabayashi, Sun Kyu KIM
Принадлежит: University of Michigan

A passive and reusable preconcentrator/injector device for measuring gas-phase analytes and methods of use. The device includes an upper plate defining an array of micro-scale diffusion channels and a lower plate secured to the upper plate. The lower plate defines a cavity for a reusable collection material in fluid communication with the micro-scale diffusion channels. An integral heating unit is provided adjacent the lower plate and configured for heating the cavity. A loading port may be included for introducing the reusable collection material into the cavity. An inlet port and an outlet port are provided, both in fluid communication with the cavity. The device may include a fluidic manifold system comprising a plurality of conduits disposed between the adsorbent cavity and the outlet port.

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Номер записи: 18
08-08-2013 дата публикации

ELEMENTAL ANALYZER

Номер: US20130199268A1
Автор: HIRANO Akihiro, Yamada Takahiro
Принадлежит: HORIBA, LTD.

In order to provide an elemental analyzer that, without providing a buffer tank, can cope with measurements of a low concentration sample to a high concentration sample on the basis of a simple configuration, the elemental analyzer is provided with: an extraction furnace adapted to heat a sample contained in a crucible R, and gasify an element contained in the sample into sample gas; an introduction flow path L adapted to introduce carrier gas into the extraction furnace a lead-out flow path L adapted to, from the extraction furnace, lead out mixed gas in which the sample gas and the carrier gas are mixed; an elemental analysis part that is provided in the lead-out flow path L and analyzes elements contained in the mixed gas; a bypass flow path L that branches from the introduction flow path L and joins the lead-out flow path L and a valve that is provided in the bypass flow path L and can adjust an opening level. 1. An elemental analyzer comprising:an extraction furnace adapted to heat a sample contained in a crucible, and gasify an element contained in the sample into sample gas;an introduction flow path adapted to introduce carrier gas into the extraction furnace;a lead-out flow path adapted to lead out mixed gas from the extraction furnace, the mixed gas is that the sample gas and the carrier gas are mixed in;an elemental analysis part that is provided in the lead-out flow path and analyzes elements contained in the mixed gas;a bypass flow path that branches from the introduction flow path and joins the lead-out flow path; anda valve that is provided in the bypass flow path and whose opening level is adjustable.2. The elemental analyzer according to claim 1 , wherein the opening level of the valve is adjusted such that a concentration of the sample gas in the mixed gas in the elemental analysis part becomes equal to a predetermined concentration.3. The elemental analyzer according to claim 1 , whereinthe opening level of the valve is adjusted such that a first ...

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Номер записи: 19
08-08-2013 дата публикации

ANALYSIS OF MOLECULAR CONTAMINATION IN VACUUM ENVIRONMENTS

Номер: US20130199269A1
Автор: De Bokx Pieter Klaas, Knobel Hugo Hubertus, Soers Ruud Johannes Theodorus
Принадлежит: KONINKLIJKE PHILIPS ELECTRONICS N.V.

A pre-concentration device is provided for a gas analysis system () for collecting molecular contamination in a vacuum environment (). The pre-concentration device () comprises a hollow element () having an entrance opening () for receiving molecules from the vacuum environment () in a collection phase, a gas outlet for transferring collected molecules to a vacuum compatible detector or second preconcentration device in a transfer phase. The device has an inner wall for adsorbing molecules in the collection phase and desorbing molecules in the transfer phase. The device has a filler element () that is movable from a first position outside the hollow element in the collection phase to a second position inside the hollow element in the transfer phase which second position leaves open a transfer channel to the gas outlet along the inner wall. Advantageously, the device enables transferring of the organic or inorganic contaminants collected in the device under vacuum conditions, and requires a minimal amount of ultra pure gas for the transport of the contaminants to a detector or further a concentration device, which lowers the lower limit of detection. 1. Pre-concentration device for a gas analysis system for detecting molecular contamination in a vacuum environment , the pre-concentration device comprising:a hollow element having a gas entrance opening for receiving gas from the vacuum environment in a collection phase, a gas outlet for transferring gas in a transfer phase, and an inner wall for adsorbing gas in the collection phase and desorbing gas in the transfer phase, anda filler element that is movable from a first position outside the hollow element in the collection phase to a second position inside the hollow element in the transfer phase which second position leaves open a transfer channel to the gas outlet along the inner wall wherein the hollow element has an inner space bounded b the inner wall and the ace has a conical shape, and the filler element has a ...

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Номер записи: 20
08-08-2013 дата публикации

HEMOGLOBIN S ANALYSIS METHOD, HEMOGLOBIN A2 ANALYSIS METHOD, AND HEMOGLOBIN A0 ANALYSIS METHOD

Номер: US20130199277A1
Автор: Taira Hiroaki, Takayuki Oka
Принадлежит:

An object of the present invention is to provide a hemoglobin S analysis method, a hemoglobin A2 analysis method, and a hemoglobin A0 analysis method which enable even highly retentive hemoglobin S, hemoglobin A2 and hemoglobin A0 to be separated in sharp, highly symmetrical peaks by means of cation-exchange high-performance liquid chromatography. 1. A method for analyzing hemoglobin S by cation-exchange high-performance liquid chromatography ,the method comprising utilizing an eluent that contains an azide or a cyanide at a concentration of 0.1 to 50 mmol/L and has a pH of 6.80 to 7.50.2. The method for analyzing hemoglobin S by cation—according to claim 1 ,wherein the eluent contains a salt at a concentration of 500 mmol/L or lower.3. The method for analyzing hemoglobin S by cation—according to claim 1 ,wherein the eluent contains a buffering agent at a concentration of 5 to 500 mmol/L.4. A method for analyzing hemoglobin A2 by cation-exchange high-performance liquid chromatography claim 1 , the method comprising utilizing an eluent that contains an azide or a cyanide at a concentration of 0.1 to 50 mmol/L and has a pH of 6.45 to 6.85.5. The method for analyzing hemoglobin A2 according to claim 4 ,wherein the eluent contains a salt at a concentration of 500 mmol/L or lower.6. The method for analyzing hemoglobin A2 according to claim 4 ,wherein the eluent contains a buffering agent at a concentration of 5 to 500 mmol/L.7. A method for analyzing hemoglobin A0 by cation-exchange high-performance liquid chromatography claim 4 , the method comprising utilizing an eluent that contains an azide or a cyanide at a concentration of 0.1 to 50 mmol/L and has a pH of 6.00 to 6.75.8. The method for analyzing hemoglobin A0 according to claim 7 ,wherein the eluent contains a salt at a concentration of 500 mmol/L or lower.9. The method for analyzing hemoglobin A0 according to claim 7 ,wherein the eluent contains a buffering agent at a concentration of 5 to 500 mmol/L.10. The ...

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Номер записи: 21
29-08-2013 дата публикации

CHROMATOGRAPHIC MEASUREMENT APPARATUS

Номер: US20130224074A1
Автор: NISHIO Tomonori
Принадлежит: FUJIFILM Corporation

Higher economic efficiency is ensured in a chromatographic measurement even when a chromatographic measurement apparatus is used at a place with risk of contamination of testing equipment. A chromatographic measurement apparatus includes an apparatus main body and a measurement unit, wherein the apparatus main body and the measurement unit are configured to be capable of wireless communication of a signal representing measurement information obtained by the measurement unit and capable of mutual pairing setting. The apparatus main body includes a storage section for storing the measurement information, an extracting section for extracting, from a signal obtained via wireless communication, a signal from the measurement unit paired with the apparatus main body based on the pairing setting, and a display section for displaying the measurement information. 1. A chromatographic measurement apparatus for measuring a test article contained in a sample solution , the apparatus comprising:an apparatus main body; andat least one measurement unit for measuring the test article by using an insoluble carrier including a detection area capable of specifically immobilizing the test article,wherein the apparatus main body and each of the at least one measurement unit are configured to be capable of wireless communication of a signal representing measurement information obtained by the measurement unit and capable of mutual pairing setting, andthe apparatus main body comprises a storage section for storing the measurement information, an extracting section for extracting, from a signal obtained via wireless communication, a signal from the measurement unit paired with the apparatus main body based on the pairing setting, and a display section for displaying the measurement information.2. The chromatographic measurement apparatus as claimed in claim 1 , wherein the pairing setting is achieved via non-contact proximity connection.3. The chromatographic measurement apparatus as ...

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Номер записи: 22
19-09-2013 дата публикации

METHOD AND APPARATUS FOR CONTROL OF MASS COMPOSITION OF MOBILE PHASE

Номер: US20130240044A1
Автор: Kirby Peter, Shreve Joshua A.
Принадлежит: WATERS TECHNOLOGY CORPORATION

Described are a method and an apparatus for delivering a fluid having a desired mass composition. According to the method, temperatures of the fluids to be mixed are sensed and the densities of the fluids at the sensed temperatures are determined. The volume of each fluid is determined so that a mixture of the fluids at the sensed temperatures has the desired mass composition. The determined volumes of the fluids are combined to create the mixture. In one option, combining the determined volumes includes metering flows of the fluids sequentially into a common fluid channel. Alternatively, combining the determined volumes includes controlling a flow rate of each of the fluids and directing the fluids into a common fluid channel. 1. A method for delivering a fluid having a desired mass composition , the method comprising: sensing a temperature of the fluid; and', 'determining a density of the fluid at the sensed temperature;, 'for each fluid in a plurality of fluids to be mixed to have a desired mass composition at a reference temperaturedetermining a volume of each of the fluids so that a mixture of the fluids at the sensed temperatures has the desired mass composition; andcombining the determined volumes of the fluids.2. The method of wherein a volumetric composition of the mixture of the fluids at the sensed temperatures is equal to a volumetric composition of a mixture of the fluids at the reference temperature.3. The method of wherein combining the determined volumes of the fluids comprises metering flows of the fluids sequentially into a common fluid channel.4. The method of wherein sensing the temperatures of the fluids comprises sensing a single temperature proximate to a location where the determined volumes are metered.5. The method of wherein combining the determined volumes of the fluids comprises controlling a flow rate of each of the fluids and directing the fluids into a common fluid channel.6. The method of wherein sensing the temperatures of the ...

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Номер записи: 23
26-09-2013 дата публикации

METHOD FOR EVALUATING SENSORY STIMULUS COMPONENT

Номер: US20130247654A1
Автор: SAIMA Toshinori, SUGIMOTO Daisuke, Yaguchi Yoshihiro, ZAIZEN Kyoko
Принадлежит: TAKASAGO INTERNATIONAL CORPORATION

A method for evaluating a sensory stimulus component in a test component with a taste-analyzing liquid chromatograph system. The method includes: measuring the test component with the taste-analyzing liquid chromatograph system to obtain taste evaluation data; and evaluating a persistence of a sensory stimulus intensity of the test component from a continuous intensity change of the test component using the taste evaluation data as an index. 1. A method for evaluating a sensory stimulus component in a test component with a taste-analyzing liquid chromatograph system ,the method comprising:measuring the test component with the taste-analyzing liquid chromatograph system to obtain taste evaluation data; andevaluating a persistence of a sensory stimulus intensity of the test component from a continuous intensity change of the test component using the taste evaluation data as an index.2. The method according to claim 1 , wherein a plurality of similar test components is measured with the taste-analyzing liquid chromatograph system to obtain taste evaluation data claim 1 , and the test components are distinguished into similar groups to evaluate the persistence of sensory stimulus intensities of the test components using the taste evaluation data as an index.3. The method according to claim 1 , wherein the taste-analyzing liquid chromatograph system includes:(a) a taste detecting part for detecting the sensory stimulus component in the test component over a time course, which includes a plurality of taste sensors each of which has a different response characteristic; and(b) a signal processing part that processes a detection signal obtained from the plurality of the taste sensors to determine taste evaluation data concerning each sensory stimulus component.4. The method according to claim 3 , wherein the taste evaluation data is measured by using an intensity of a sensor reaction of the taste sensor as an index.5. The method according to claim 4 , wherein the evaluation ...

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Номер записи: 24
17-10-2013 дата публикации

Chromatographic Optical Detection System

Номер: US20130269424A1
Автор: Jarrell Joseph A.
Принадлежит: WATERS TECHNOLOGIES CORPORATION

A chromatographic optical detection system includes an optical detector disposed to receive light scattered from a stream of particles and configured to convert the received light to an electrical signal; a signal-processing unit in signal communication with the optical detector to receive the electrical signal, and configured to convert the electrical signal to digital pulses and count the digital pulses to output a first signal corresponding to a number of particles detected in a time interval, and configured to integrate and digitize the electrical signal to output a second signal corresponding to the number of particles detected in the time interval; and a data station in signal communication with the signal-processing unit, and configured to select the first signal, if the number of particles detected in the time interval is less than a threshold criterion, and to select the second signal if the number of particles detected in the time interval exceeds the threshold criterion. The threshold criterion is associated with a saturation condition. 1. A chromatographic optical detection system comprising:an optical detector disposed to receive light scattered from a stream of particles and configured to convert the received light to an electrical signal;a signal-processing unit in signal communication with the optical detector to receive the electrical signal, and configured to convert the electrical signal to digital pulses and count the digital pulses to output a first signal corresponding to a number of particles detected in a time interval, and to integrate and digitize the electrical signal to output a second signal corresponding to the number of particles detected in the time interval; anda data station in signal communication with the signal-processing unit, and configured to select the first signal, if the number of particles detected in the time interval is less than a threshold criterion, and to select the second signal, if the number of particles detected ...

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Номер записи: 25
24-10-2013 дата публикации

METHODS FOR DIAGNOSING AND ASSESSING KIDNEY DISEASE

Номер: US20130276513A1
Автор: NAVIAUX Robert, SHARMA Kumar
Принадлежит:

The technology relates in part to methods for identifying the presence of kidney disease, determining the level of kidney disease, or the progression of kidney disease, in a subject that has or has not been diagnosed with diabetes. The technology further relates to methods for determining the targets for therapy for kidney disease, the efficacy of a treatment for kidney disease, and methods for determining the toxicity of a therapeutic in a subject with kidney disease. The technology relates in part to methods for identifying the presence of kidney disease, determining the level of kidney disease, or the progression of kidney disease, in a subject that has or has not been diagnosed with diabetes. The technology further relates to methods for determining the targets for therapy for kidney disease, the efficacy of a treatment for kidney disease, and methods for determining the toxicity of a therapeutic in a subject with kidney disease. 1. A method of identifying the presence or level of kidney disease in a subject , comprisinga. determining the level of at least one organic acid selected from the group consisting of glycolic acid, 3-OH isobutyric acid, 3-OH isovaleric acid, aconitic acid, homovanillic acid, citric acid, uracil, fumaric acid, oleic acid and azelaic acid in a sample obtained from the subject; i. the reference level has been determined from at least one sample collected from the same subject at a different time period; or', 'ii. the reference level has been determined from a sample or samples collected from one or more other subjects; and, 'b. comparing the level of the at least one organic acid with a reference level of the at least one organic acid, wherein'}c. identifying the presence or level of kidney disease in the subject where the at least one organic acid level in the subject is decreased when compared to the reference level of the at least one organic acid.2. The method of claim 1 , wherein the level of:(a) at least two organic acids selected ...

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Номер записи: 26
14-11-2013 дата публикации

FITTING ELEMENT WITH BIO-COMPATIBLE SEALING

Номер: US20130298647A1
Автор: Falk-Jordan Stefan
Принадлежит: AGILENT TECHNOLOGIES, INC.

A fitting element is configured for coupling tubing to a fluidic device having a receiving cavity configured for receiving the fitting element, where the tubing has an inner contact surface of a biocompatible material, the inner contact surface being configured to contact a fluid to be conducted by the tubing, and the receiving cavity having a receiving contact surface of a bio-compatible material. The fitting element includes a first sealing element of a bio-compatible material configured for sealing to the bio-compatible material of the inner contact surface of the tubing, and a second sealing element configured for sealing against a pressure ambient to a pressure of the fluid in the tubing. Upon coupling of the tubing to the fluidic device, at least a portion of the receiving contact surface, the first sealing element, and the second sealing element enclose an interspace, each surface of the interspace being a bio-compatible material. 1. A fitting element for coupling a tubing to a fluidic device having a receiving cavity configured for receiving the fitting element , wherein the tubing has an inner contact surface comprising a biocompatible material , the inner contact surface being configured to contact a fluid to be conducted by the tubing , and the receiving cavity having a receiving contact surface comprising a bio-compatible material , the fitting element comprising:a first sealing element comprising a bio-compatible material and being configured for sealing to the bio-compatible material of the inner contact surface of the tubing, anda second sealing element configured for sealing against a pressure ambient to a pressure of the fluid in the tubing,wherein, upon coupling of the tubing to the fluidic device, at least a portion of the receiving contact surface, the first sealing element, and the second sealing element enclose an interspace, each surface of the interspace comprising a bio-compatible material.2. The fitting element of claim 1 , wherein the ...

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Номер записи: 27
21-11-2013 дата публикации

GELC-MS USING STAIN FREE TECHNOLOGY

Номер: US20130306854A1
Автор: Belisle Christopher
Принадлежит:

Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment. 1. A method of preparing a protein sample for mass spectroscopy , the method comprising:providing an electrophoresis gel comprising the protein sample, wherein proteins of the protein sample have been separated by electrophoresis;contacting the protein sample with a halo-substituted compound;exposing the electrophoresis gel to UV light;detecting fluorescence emitted from the electrophoresis gel;excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; andsubjecting proteins from the at least one portion to mass spectroscopy.2. The method of claim 1 , wherein the halo-substituted compound is a component of the electrophoresis gel and said contacting occurs upon separating proteins of the protein sample by electrophoresis.3. The method of claim 1 , wherein the halo-substituted compound is selected from the group consisting of chloroform claim 1 , trichloroethanol claim 1 , trichloroacetate claim 1 , and 3-bromo-1-propanol.4. The method of claim 1 , wherein the UV light has a wavelength in the range of about 200 nm to about 400 nm.5. The method of claim 1 , wherein the fluorescence emitted from the ...

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Номер записи: 28
05-12-2013 дата публикации

BUBBLE REDUCTION DEVICE, CHROMATOGRAPHY DEVICE, BUBBLE REDUCTION METHOD, AND BUBBLE REDUCTION PROGRAM

Номер: US20130319087A1
Автор: Kasai Tokuo, MATSUBARA Takeshi, SATAKE Seiji, Sezaki Akira
Принадлежит: ARKRAY, INC.

A bubble reduction device, chromatography device, bubble reduction method and bubble reduction program capable of reducing bubbles in an eluent. Included are a liquid accommodation portion, a liquid supply apparatus, an air layer formation apparatus, a first channel and an evacuation portion. The liquid accommodation portion accommodates a liquid that is to elute an analysis component from a specimen adsorbed to an adsorption portion. The liquid supply apparatus, by operation of a rod pushing up and polling down, sucks and discharges the liquid through an aperture portion of a tube portion, the aperture portion being oriented upward. The air layer formation apparatus forms an air layer in the tube portion. The first channel connects the liquid supply apparatus with the liquid accommodation portion. The evacuation portion is connected to the first channel via a first switching valve and evacuates the air layer through the first channel. 1. A bubble reduction device comprising:a liquid accommodation portion that accommodates a liquid that is to elute an analysis component from a specimen adsorbed to an adsorption portion;a liquid supply apparatus that, by operation of a rod pushing up or pulling down, sucks or discharges the liquid through an aperture portion of a tube portion, the aperture portion being oriented upward;an air layer formation apparatus that forms an air layer in the tube portion;a first channel that connects the liquid supply apparatus with the liquid accommodation portion; andan evacuation portion that is connected to the first channel via a first switching valve and that evacuates the air layer through the first channel.2. The bubble reduction device according to claim 1 , wherein the air layer formation apparatus includes an atmosphere release valve provided at the first channel claim 1 , andthe air layer is introduced into the tube portion through the first channel by an operation of the rod pushing up or pulling down in a state in which the ...

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Номер записи: 29
05-12-2013 дата публикации

LIQUID CHROMATOGRAPHY APPARATUS, LIQUID CHROMATOGRAPHY ANALYSIS METHOD, AND LIQUID CHROMATOGRAPHY ANALYSIS PROGRAM

Номер: US20130319088A1
Автор: Kasai Tokuo, SATAKE Seiji
Принадлежит: ARKRAY, INC.

A liquid chromatography apparatus having: a column that adsorbs analysis components within a specimen; a plunger pump that feeds eluent A, that elutes the analysis components adsorbed at the column, in an amount greater than or equal to an amount needed for analysis of one specimen from a cylinder portion by a one-time pushing operation of a rod; a photometric unit that analyzes analysis components eluted by eluent A; an eluent loop that holds eluent B; a liquid feeding flow path that communicates the plunger pump and the column; and a first switching valve that switches the liquid feeding flow path to either of a first flow path, that causes eluent A to flow from the plunger pump to the column, and a second flow path, that causes eluent A to flow from the plunger pump through the first eluent holding loop to the column, is provided. 1. A liquid chromatography apparatus comprising:an adsorbing portion that adsorbs analysis components within a specimen;a liquid feeding device that feeds a first eluent, that elutes analysis components adsorbed at the adsorbing portion, in an amount greater than or equal to an amount needed for analysis of one specimen from a cylinder portion by a one-time pushing operation of a rod;a liquid feeding flow path that communicates the liquid feeding device and the adsorbing portion; andan analyzing unit for analyzing analysis components eluted by the first eluent.2. The liquid chromatography apparatus of claim 1 , further comprising:a first holding flow path that holds a second eluent that is different than the first eluent; andfirst switching unit for switching the liquid feeding flow path to either of a first flow path, that causes the first eluent to flow from the liquid feeding device to the adsorbing portion, and a second flow path, that causes the first eluent to flow from the liquid feeding device through the first holding flow path to the adsorbing portion.3. The liquid chromatography apparatus of claim 2 , further comprising:a ...

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Номер записи: 30
05-12-2013 дата публикации

Device and Method for Field Flow Fractionation

Номер: US20130319945A1
Автор: Johann Christoph, Rosch Ulrich
Принадлежит: WYATT TECHNOLOGY EUROPE GMBH

A device for field flux fractioning comprises a sensor to determine the presence of particles in a liquid and a channel impermeable for particles and permeable for liquid. A pump conveys the liquid to first and second paths to the channel, where the second path connects in a first position to the pump outlet and in a second position to the sensor. An injection device injects a sample comprising particles into the liquid flowing through the first path. A distribution device distributes the flow volume conveyed by the pump in a first position at a predetermined ratio to the first and second paths. A control device comprises a valve and a measuring device to measure the flow volume. The valve controls the flow volume in the first path in consideration of the measuring device measurement and the pump conveys the liquid in a flow volume, which can be precisely dosed. 1. A device for field flux fractioning , witha sensor for determining the presence of particles in a carrier liquid,at least one essentially closed oblong channel, which comprises a first end with a first connection and a second end with a second connection and its wall comprises at least one section extending in the longitudinal direction of the channel, impermeable for the particles but permeable for the carrier liquid,a pump, which comprises an inlet and an outlet and is provided to convey the carrier liquid,a first liquid path, which connects the outlet of the pump to a first connection of the channel,a second liquid path, which connects the second connection of the channel to a first switching means, through which the second liquid path can be connected in a first switch position to the outlet of the pump and in a second switch position to the sensor,an injection device, which is arranged in the first liquid path and provided to inject a sample comprising particles into the carrier liquid flowing through the first liquid path, anda flow volume—distribution device, which divides the flow volume conveyed ...

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Номер записи: 31
12-12-2013 дата публикации

SAMPLE PREPARATION BY SOLID PHASE EXTRACTION

Номер: US20130330249A1
Автор: Dawes Ernest Frederick, Gooley Andrew
Принадлежит:

A flow control assembly for use in preparation of samples for analysis by solid phase extraction includes a housing assembly having a first passage communicating with a needle bore and locating a solid phase sorbent, a second fluid port, and a valve for selective aspiration or delivery of fluid across a solid phase sorbent, or bypassing the solid phase sorbent. Independent flow paths are provided for a sample via the needle, and of the elution solvent via a separate port. The valve includes relatively slideable first and second members, the second member including an elongate shank that is relatively reciprocably slideable for effecting selective aspiration or delivery of fluid between first and second relative positions. A third fluid port may be provided communicating with the elongate second passage for selective fluid communication with a port in a shank when the shank is at the second relative position. 1. A flow control assembly for use in preparation of samples for analysis by solid phase extraction , comprising:a housing assembly adapted to be fitted as a coupling between a syringe barrel having a barrel chamber therein and a syringe needle that has a needle bore extending therealong and defines a first fluid port at its tip, which housing assembly includes structure defining a first passage arranged to be in fluid communication with the needle bore at its inner end and to locate a solid phase sorbent that contacts fluid traversing said first passage and adsorbs predetermined species;a second fluid port in said housing assembly; anda valve arrangement for selectively communicating the barrel chamber with said first passage and thereby with the needle bore or with the second fluid port for facilitating selective aspiration or delivery of fluid to or from said first fluid port across said solid phase sorbent or to or from said second fluid port bypassing the solid phase sorbent.2. A flow control assembly according to wherein said valve arrangement comprises ...

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Номер записи: 32
12-12-2013 дата публикации

CARRIER-ENCLOSED TRANSFORMABLE CONTAINER, CARRIER-ENCLOSED TRANSFORMABLE CONTAINER PROCESSING APPARATUS, AND CARRIER-ENCLOSED TRANSFORMABLE CONTAINER PROCESSING METHOD

Номер: US20130330834A1
Автор: Tajima Hideji
Принадлежит: Universal Bio Research Co., Ltd.

Provided are a carrier-enclosed transformable container, a carrier-enclosed transformable container processing apparatus, and a carrier-enclosed transformable container processing method about which a carrier to which various substances such as biogenic substances are bonded or bondable is held in a transformable container as the container to be in a substantially stationary state, thereby making it possible to make the handling of the carrier, measurement, and other treatments effective, speedy, and easy. The container is formed to have: a containing part which can contain liquid and gas at its inside surrounded by a wall face, a part of this wall face having a transformable wall face capable of undergoing a predetermined transformation without changing the entire internal surface area of the wall face substantially; an orifice part which is connected to the containing part and can undergo the inflow and outflow of a liquid sucked and discharged by the expansion and contraction of the inside by the transformation of the transformable wall face, respectively; and a carrier to which a predetermined substance enclosed in the containing part to be in a substantially stationary state is bonded or bondable. 1. A carrier-enclosed transformable container processing method , comprising: a supporting step of supporting , onto a carrier enclosed head , two or more carrier-enclosed transformable containers each having a containing part which can contain liquid and gas at its inside surrounded by a wall face , a part of this wall face having a transformable wall face capable of undergoing a predetermined transformation without changing the entire internal surface area of the wall face substantially , an orifice part which is connected to the containing part and can undergo the inflow and outflow of a liquid sucked and discharged by the expansion and contraction of the inside by the transformation of the transformable wall face , respectively , and a predetermined carrier , to ...

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Номер записи: 33
19-12-2013 дата публикации

MOISTURE SENSOR INCLUDING, AS A MOISTURE-ABSORBING LAYER, A POLYMER LAYER INCLUDING A MIXTURE OF POLYAMIDES

Номер: US20130336842A1
Автор: Grange Hubert
Принадлежит: Commissariat A L'Energie Atomique Et Aux Energies Alternatives

The invention relates to a humidity sensor including, as a humidity absorbent layer, a polymer layer including a blend including a first polyamide and a second polyamide, where the said second polyamide includes, in its repetitive units, a number of carbon atoms greater than that of the repetitive units of the first polyamide. 1. A humidity sensor including , as a humidity absorbent layer , a polymer layer including a blend including a first polyamide and a second polyamide , where the said second polyamide includes , in its repetitive units , a number of carbon atoms greater than that of the repetitive units of the first polyamide.2. A humidity sensor according to claim 1 , in which the polymer layer consists solely of polyamides.3. A humidity sensor according to claim 1 , wherein the first polyamide is chosen from among polyamide 6 claim 1 , polyamide 6-6 and polyamide 11 claim 1 , and the second polyamide is chosen from among polyamide 6-6 claim 1 , polyamide 6-10 and polyamide 12.4. A humidity sensor according to claim 1 , wherein the polymer layer includes a blend chosen from among the following blends:a blend of polyamide 6 and polyamide 6-6;a blend of polyamide 6 and polyamide 6-10;a blend of polyamide 6-6 and polyamide 6-10;a blend of polyamide 11 and polyamide 12.5. A humidity sensor according to claim 1 , wherein the polymer layer includes a blend of polyamide 6 and polyamide 6-6.6. A humidity sensor according to claim 1 , wherein claim 1 , in the blend claim 1 , the first polyamide is present relative to the second polyamide in a mass proportion of between 95/5 and 5/95.7. A humidity sensor according to claim 1 , including at least one said polymer layer claim 1 , positioned between a first electrode and a second electrode.8. A humidity sensor according to claim 7 , in which the first electrode and the second electrode are in contact with the same substrate.9. A humidity sensor according to claim 1 , including a beam or a membrane covered claim 1 , wholly ...

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Номер записи: 34
02-01-2014 дата публикации

AUTOMATED ANALYZER

Номер: US20140005952A1
Автор: HASHIMOTO Yoshiaki, Okada Noriyoshi
Принадлежит: Beckman Coulter, Inc.

An analyzer, and a method and an apparatus for detecting liquid overflowing from a container that the analyzer comprises, are provided. The method according to the present invention for detecting liquid overflowing from a container in the analyzer, includes a step of judging that liquid is overflowing from at least one container if the difference between a standard deviation of absorbance of the liquid measured at a plurality of points of a container at time T and a standard deviation of absorbance of the liquid measured at a plurality of points in said container at time T is greater than a predetermined threshold value. 18.-. (canceled)9. A method for detecting liquid overflowing from at least one of a plurality of containers comprised in an analyzer , each container containing liquid , the method comprising the steps of: measuring an absorbance of the liquid contained in the one container at a plurality of points of the one container at a first time corresponding to the one container;', 'calculating a standard deviation of the absorbance of the liquid measured at the plurality of points of the one container at the first time corresponding to the one container, as a first standard deviation;', 'measuring an absorbance of the liquid contained in the one container at a second time corresponding to the one container;', 'calculating a standard deviation of the absorbance of the liquid measured at the plurality of points of the one container at the second time corresponding to the one container, as a second standard deviation; and', 'comparing a difference between the first standard deviation and the second standard deviation, with a predetermined threshold value; and, 'for each one of N-number of containers among the plurality of containers,'}judging that the liquid is overflowing from at least one of the plurality of containers if the difference between the first standard deviation and the second standard deviation is greater than the predetermined threshold value in ...

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Номер записи: 35
16-01-2014 дата публикации

CHROMATOGRAPHIC INTERFACE

Номер: US20140014585A1
Автор: Dourdeville Theodore A., Grigat Rolf, Phoebe Charles
Принадлежит: WATERS TECHNOLOGIES CORPORATION

An apparatus for chromatographic interfacing includes an interfacing unit, a sample acquisition unit, and a sample trapping unit. The interfacing unit includes (i) a chromatographic inflow valve adapted to receive a chromatographic sample separation flow, (ii) a chromatographic outflow valve adapted to allow discharge of the sample separation flow, (iii) a loop expulsion inflow valve adapted to receive an expulsion fluid flow, and (iv) a loop expulsion outflow valve in fluid communication with the loop expulsion inflow valve and adapted to allow discharge of a fluid. The sample acquisition unit includes (i) an inflow selector in selectable fluid communication with the chromatographic inflow valve or the loop expulsion inlet valve, (ii) an outflow selector in fluid communication with the loop expulsion outflow valve, and (iii) one or more collection loops, each loop being independently selectable to establish fluid communication between the inflow selector valve and the outflow selector valve. The sample trapping unit includes (i) a trap in selectable fluid communication with the loop expulsion outflow valve and defining a flow path from a first port to a second port and through a stationary phase disposed therebetween, the stationary phase adapted to trap an analyte of interest from a fraction of the chromatographic sample separation flow, and (ii) a scavenging gas source in selectable fluid communication with the flow path and a vacuum source in selectable fluid communication with the flow path, the scavenging gas source and vacuum source adapted to substantially dry the stationary phase. 1. An apparatus for chromatographic interfacing comprising: an interfacing unit including(i) a chromatographic inflow valve adapted to receive a chromatographic sample separation flow,(ii) a chromatographic outflow valve adapted to allow discharge of the sample separation flow,(iii) a loop expulsion inflow valve adapted to receive an expulsion fluid flow, and 'a sample acquisition ...

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Номер записи: 36
16-01-2014 дата публикации

METHOD AND MEANS FOR SAMPLE PREPARATION

Номер: US20140017813A1
Автор: GLAD GUNNAR, Johansson Bo-Lennart, MALOISEL JEAN-LUC
Принадлежит: GE HEALTHCARE BIO-SCIENCES AB

The present invention relates to a method for depletion of undesired molecules and/or enrichment of desired molecules from a sample comprising high abundant as well as low abundant molecules, comprising the following steps: a) providing a separation material comprising a solid phase (beads) comprising an inner porous core material comprising magnetic particles and an outer porous shell with a porosity equal or denser than that of the shell; b) adding the sample to the separation material; c) adsorbing a first fraction of molecules with a molecular weight of 500-50 000 Da in the core and simultaneously excluding a second fraction of molecules from binding to the core and the shell, wherein the molecular weight of the second fraction molecules is at least 5 preferably 10 times higher than the molecular weight of the first fraction and d) eluting the desired molecules from the separation material, wherein step d) and optionally step c) is performed using an oscillating power/field applied over the separation material. 1. A method for depletion of undesired molecules and/or enrichment of desired molecules from a sample comprising high abundant as well as low abundant molecules , comprising the following steps:a) providing a separation material comprising a solid phase (beads) comprising an inner porous core material comprising magnetic particles and an outer porous shell with a porosity equal or denser than that of the shell;b) adding the sample to the separation material;{'b': 5', '10, 'c) adsorbing a first fraction of molecules with a molecular weight of 500-50 000 Da in the core and simultaneously excluding a second fraction of molecules from binding to the core and the shell, wherein the molecular weight of the second fraction molecules is at least preferably times higher than the molecular weight of the first fraction and'}d) eluting the desired molecules from the separation material, wherein step d) and optionally step c) is performed using an oscillating power/ ...

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Номер записи: 37
23-01-2014 дата публикации

TRACE ANALYTE EXTRACTION USING ADSORPTIVE CARBIDE-DERIVED NANOPOROUS CARBON POWDERS

Номер: US20140020447A1
Автор: Badorrek Christopher S., Burton Robert A., Kippeny Tadd C., Sengupta Louise C., Swafford Laura A.
Принадлежит: BAE Systems Information and Electronic Systems Integration Inc.

In the method of latent print analysis, wherein the improvement comprises using an ultra-fine, nanoporous carbon material as an adsorptive powder to identify trace chemicals found within oils of a print. 1. A method of chemical analyte analysis comprising the steps of utilizing a hyperadsorbent carbon material having a plurality of nanopores; collecting latent fingerprints; extracting analytes with a supercritical carbon dioxide solvent from a latent fingerprint; and analyzing analytes with liquid chromatography.2. The method of wherein said hyperadsorbent carbon material further comprises: a particle size of about 5 um; and a surface area of about 2500 m/g having a mean anopore diameter of about 5 nm.3. The method of wherein extracting analytes with a supercritical carbon dioxide solvent from a latent fingerprint further comprises utilizing a front end supercritical carbon dioxide extraction system.4. The method of wherein extracting analytes with a supercritical carbon dioxide solvent from a latent fingerprint further comprises extracting the analytes without disturbing said latent fingerprint.5. A method of forensic analysis comprising the steps of: utilizing a hyperadsorbent carbon material claim 2 , said hyperadsorbent carbon material having a mean nanopore diameter within the range of about 1 nm to about 5 nm;collecting a latent fingerprint; extracting analytes from a latent fingerprint; and analyzing analytes.6. The method of wherein said hyperadsorbent carbon material comprises a carbide-derived carbon skeleton and a functional group.7. The method of wherein the functional group is selected from the group consisting of metal carbide claim 6 , molybdenum claim 6 , silicon claim 6 , and/or titanium.8. The method of wherein said hyperadsorbent carbon material further comprises a particle size within the range of about 5 um to about 10 um; and a surface area of about 1 claim 7 ,000 m/g to about 2500 m/g having a mean nanopore diameter of 5 nm.9. The method of ...

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Номер записи: 38
23-01-2014 дата публикации

Systems and Methods for Measuring Total Sulfur Content in a Fluid Stream

Номер: US20140024129A1
Автор: Meyer Randy James, Meyer Shirley Ann, Smith Stevie Horton
Принадлежит: Cameron International Corporation

A system for determining the content of sulfur in a first fluid mixture includes a gas chromatograph including a sample valve, a first column coupled to the sample valve, and a second column coupled to the sample valve. In addition, the system includes a pyrolizer configured to subject the first fluid mixture to pyrolysis to produce a second fluid mixture that includes hydrogen sulfide. The first column is configured to separate at least a first constituent of the second fluid mixture from the hydrogen sulfide in the second fluid mixture and output a third fluid mixture including the hydrogen sulfide. The second column is configured to separate at least a second constituent in the third fluid mixture from the hydrogen sulfide in the third fluid mixture and output a fourth fluid mixture including the hydrogen sulfide. Further, the system includes a detector in fluid communication with the second column. 1. A system for determining the content of sulfur in a first fluid mixture including one or more sulfur compounds , the system comprising:a gas chromatograph including a sample valve configured to receive the first fluid mixture, a first column coupled to the sample valve, and a second column coupled to the sample valve;a pyrolizer coupled to the sample valve, wherein the pyrolizer is configured to subject the first fluid mixture to pyrolysis to produce a second fluid mixture that includes hydrogen sulfide;wherein the first column is configured to receive the second fluid mixture from the pyrolizer and separate at least a first constituent of the second fluid mixture from the hydrogen sulfide in the second fluid mixture and output a third fluid mixture including the hydrogen sulfide;wherein the second column is configured to receive the third fluid mixture from the first column and separate at least a second constituent in the third fluid mixture from the hydrogen sulfide in the third fluid mixture and output a fourth fluid mixture including the hydrogen sulfide; anda ...

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Номер записи: 39
23-01-2014 дата публикации

MAGNETIC PARTICLE SEPARATOR

Номер: US20140024136A1
Автор: Chappell Derek L., Conway Donald V., Moriarity John
Принадлежит:

A magnetic particle separator () for use with a microplate () in methods employing magnetic particles, said magnetic particle separator ()comprising a plurality of magnets () secured to a base plate () by a plurality of spacers (), wherein the first ends of said plurality of spacers (a) are secured to the base plate and the second ends of said plurality of spacers (b) are secured to said plurality of magnets () and whereby said plurality of magnets () are separated from said base plate () by a distance of at least 15 mm. A magnetic particle separation system () comprising a magnetic particle separator () and a microplate () are also disclosed. Also disclosed are methods of using the magnetic particle separator () and magnetic particle separation system () to separate magnetic particles and bound antibodies. 1. A magnetic particle separator for use with a microplate , comprising:a) a base plate;b) a plurality of magnets; andc) a plurality of spacers, each having a first end and a second end;d) wherein the first ends of said plurality of spacers are secured to the base plate and the second ends of said plurality of spacers are secured to said plurality of magnets;e) whereby said plurality of magnets are separated from said base plate by a distance of at least 15 mm.2. The magnetic particle separator of claim 1 , wherein the plurality of magnets are diametrically charged.3. The magnetic particle separator of claim 1 , wherein each of the plurality of spacers is cylindrical.4. The magnetic particle separator of claim 1 , wherein there are at least twenty-four magnets secured to at least twenty four spacers claim 1 , said spacers being secured to said base plate claim 1 , for use with a ninety-six well microplate.5. A magnetic particle separation system comprising:a) a microplate having a plurality of wells for holding a plurality of biological samples containing magnetic particles and recesses being formed between the plurality of wells; andb) a magnetic particle ...

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Номер записи: 40
30-01-2014 дата публикации

SOLID-PHASE MICROEXTRACTION

Номер: US20140030818A1
Автор: SCHUELER Kai Heinrich, ZUMBACH Melchior
Принадлежит: CTC Analytics AG

In a method for performing a solid-phase micro-extraction, a holding device connected to an extraction device and secured on an adapter is held, while an actuating device connected to a guide device is moved. A device for performing a solid-phase microextraction comprises an actuating device connected to a guide device, and a holding device connected to an extraction device, wherein the extraction device is guided at least partially in the guide device, and wherein the extraction device is movable relative to the guide device by means of an actuation of the actuating device. 1. Method for performing a solid-phase micro-extraction , wherein a holding device connected to an extraction device and secured on an adapter is held , while an actuating device connected to a guide device is moved.2. Method according to claim 1 , characterized in that the actuating device is transferred from a first position claim 1 , in which the extraction device does not protrude beyond a distal end of the guide device claim 1 , to a second position claim 1 , in which the extraction device protrudes at least partially beyond a distal end of the guide device.3. Method according to claim 2 , characterized in that the actuating device is transferred from the first position to the second position with a motor drive.4. Method according to claim 1 , characterized in that the actuating device is moved by motor from a second position claim 1 , in which the extraction device protrudes at least partially beyond a distal end of the guide device claim 1 , to a first position claim 1 , in which the extraction device does not protrude beyond a distal end of the guide device.5. Device for performing a solid-phase micro-extraction claim 1 , comprising an actuating device connected to a guide device claim 1 , and a holding device connected to an extraction device claim 1 , wherein the extraction device is guided at least partially in the guide device claim 1 , and wherein the extraction device is movable ...

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Номер записи: 41
13-02-2014 дата публикации

Method for Harvesting Nanoparticles and Sequestering Biomarkers

Номер: US20140045274A1
Автор: Bishop Barney, Dunlap Thomas M., Fredolini Claudia, Kunkel Alessandra Luchini, Liotta Lance, Meani Francesco, Patanarut Alexis, Petricoin Emanuel
Принадлежит:

Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid. 1. A method of capturing analytes in a bodily fluid , comprising:mixing a solution containing open-meshwork hydrogel capture particles that contain an analyte binding affinity molecule with a fluid solution that contains an analyte of interest.2. The method of claim 1 , wherein the capture particles are comprised of N-isopropylacrylamide (NIPAm).3. The method of claim 1 , wherein the open-meshwork hydrogel capture particles that contain an analyte binding affinity molecule are covalently coupled within a volume of the open meshwork hydrogel capture particles.4. The method of claim 1 , further comprising the analyte binding affinity molecule capturing the analyte of interest against a concentration gradient resulting in a higher analyte of interest concentration within the volume of the open-meshwork hydrogel capture particles.5. The method of claim 1 , further comprising the analyte binding affinity molecule capturing the analyte of interest.6. The method of claim 2 , wherein the open-meshwork hydrogel capture particles that contain an analyte ...

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Номер записи: 42
20-02-2014 дата публикации

Sample Component Trapping, Release, and Separation with Membrane Assemblies Interfaced to Electrospray Mass Spectrometry

Номер: US20140048700A1
Автор: White Thomas P., Whitehouse Craig M.
Принадлежит:

A method and apparatus to trap, release and/or separate sample components in solution passing through a channel with or without packing material present by passing ion current through the channel driven by an electric field. A portion of the ion current includes cation and/or anion species generated from second solution flows separated from the sample solution flow path by semipermeable membranes. Cation and/or anion species generated in the second solution flow regions are transferred into the sample solution flow path through ion selective semipermeable membranes. Ion current moving along the sample solution flow path is controlled by varying the composition of the second solutions and/or changing the voltage between membrane sections for a given sample solution composition. The sample composition may also be varied separately or in parallel to enhance trapping, release and/or separation efficiency and range. 1. (canceled)2. An apparatus for analyzing chemical species , comprising:a. at least one membrane assembly each configured with a semipermeable membrane separating a first flow channel and a second flow channel, and an electrode positioned in the second flow channel,b. a first solution flow and a second solution flow in the first and second flow channels, respectively,c. an ion source interfaced to a first solution flow outlet of the at least one membrane assembly, and a mass spectrometer,d. means for introducing sample species into the first solution, ande. a chromatography column or an electrically insulated open channel connected downstream of the first solution flow outlet.3. The apparatus according to claim 2 , further comprising a detector to detect the sample species eluting from the at least one membrane assembly.4. The apparatus according to claim 2 , wherein the ion source comprises an electrospray ion source.5. The apparatus according to claim 1 , further comprising means for applying and controlling voltages applied to the electrode.6. The ...

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Номер записи: 43
20-02-2014 дата публикации

BIOSENSOR AND BIOMATERIAL DETECTION APPARATUS INCLUDING THE SAME

Номер: US20140050621A1
Автор: Jang Won Ick, JEONG Eun-Ju, Kim Yark Yeon, LIM Ji Eun, YOON Yong Sun, Yu Han Young
Принадлежит:

Provided are a biosensor and a biomaterial detection apparatus including the same. The biomaterial detection apparatus comprises a light source to provide quantized photons; a substrate spaced apart from the light source; a single photonic sensor layer disposed on the substrate to sense the photons; and an adsorption layer disposed to cover the single photonic sensor layer, allow the photons to pass therethrough, and adsorb a biomaterial between the light source and the substrate. 1. A biomaterial detection apparatus comprising:a light source providing quantized photons;a substrate spaced apart from the light source;a single photonic sensor layer disposed on the substrate to sense the photons; andan adsorption layer covering the single photonic sensor layer, allowing the photons to pass therethrough, and adsorbing a biomaterial between the light source and the substrate.2. The biomaterial detection apparatus as set forth in claim 1 , wherein the single photonic sensor layer comprises an avalanche photodiode or a silicon photomultiplier.3. The biomaterial detection apparatus as set forth in claim 1 , wherein the adsorption layer comprises silicon or silicon oxide.4. The biomaterial detection apparatus as set forth in claim 1 , wherein the adsorption layer comprises at least one of glass claim 1 , quartz claim 1 , silicon nitride (SiN) claim 1 , germanium nitride (GeN) claim 1 , aluminum oxide (AlO) claim 1 , aluminum sulfide (AlS) claim 1 , gallium sulfide (GaS) claim 1 , indium sulfide (InS) claim 1 , aluminum selenide (AlSe) claim 1 , gallium selenide (GaSe) claim 1 , indium selenide (InSe) claim 1 , aluminum telluride (AlTe) claim 1 , gallium telluride (GaTe) claim 1 , indium telluride (InTe) claim 1 , aluminum cobalt (AlCO) claim 1 , polycarbonate claim 1 , poly(methyl methacrylate) (PMMA) claim 1 , and cyclic olefin copolymer (COC).5. The biomaterial detection apparatus as set forth in claim 1 , wherein the adsorption layer comprises a DNA adsorption layer.6. ...

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Номер записи: 44
06-03-2014 дата публикации

Device for and Method of Isolating and Analyzing a fraction in a Biological Sample

Номер: US20140065622A1
Автор: Beebe David J., Berry Scott M., Casavant Benjamin P., Lang Joshua M., Strotman Lindsay N.
Принадлежит:

A device and a method are provided for isolating a fraction in a biological sample. The fraction is bound to solid phase substrate to define a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein to capture a desired fraction of the biological sample. A force is provided that is generally perpendicular to gravity. The force is movable between a first position adjacent the input zone multiple other positions adjacent various purification, protein analysis, separation and extraction zones. The force captures the fraction-bound solid phase substrate and the fraction-bound solid phase substrate moves from the input zone to the other zones to perform a multi-step assay on the isolated fraction within the device. 1. A method of conducting an assay on a fraction composed of selected cells in a biological sample , the method comprising the steps of:a) obtaining a biological sample;b) binding a solid phase substrate to the fraction of the sample;c) isolating the fraction from the remainder of the sample in a device by applying a force to the substrate to form an isolated fraction; andd) conducting at least a part of at least one of a protein, genomic and gene expression analysis on the isolated fraction within the device.2. The method of wherein the fraction is not directly contacted within the device from outside the device.3. The method of wherein the step of isolating the fraction comprises isolating a fraction having 1000 cells or less.4. The method of wherein the step of conducting at least one of a protein claim 1 , genomic and gene expression analysis on the isolated fraction comprises conducting a protein analysis on the isolated fraction.5. The method of wherein the protein analysis is conducted in part by staining the isolated fraction within the device.6. The method of wherein the step of conducting a protein analysis on the isolated fraction comprises the steps of:a) contacting the isolated fraction ...

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Номер записи: 45
03-04-2014 дата публикации

POLYCATIONIC QUATERNARY AMMONIUM POLYMER COATINGS FOR IMMOBILIZING BIOLOGICAL SAMPLES

Номер: US20140093909A1
Автор: Fox William A., III William Carl, Ray
Принадлежит: TriPath Imaging, Inc.

The present invention is directed to a pre-coated substrate, such as a slide, that is useful for immobilizing a sample. The invention is further provides methods of preparing such pre-coated substrates and methods of analyzing biological samples immobilized on such pre-coated substrate. The substrate is coated with a polycationic polymeric coating material specifically selected such that that coated substrate exhibits increased stability and prolonged shelf-life. Preferred polymeric coating materials include allylic or vinylic polymers having cationic groups thereon and having no more than a small percentage of peptidic monomeric linkages, particularly polydiallyldimethylammonium (PDDA). 123-. (canceled)24. A pre-coated substrate adapted for immobilizing a biological sample for analysis , the substrate comprising a surface having a plurality of anionic groups , wherein the substrate is coated with a non-peptidic polymeric material comprising a plurality of cationic groups , and wherein the pre-coated substrate surface is capable of immobilizing an average number of cells per surface area of at least about 20 ,000 cells/cmwhen the pre-coated substrate is contacted with 1 ml of a suspension of cells from the SiHa cell line.25. The pre-coated substrate according to claim 24 , wherein the pre-coated substrate surface is capable of immobilizing an average number of cells per surface area of at least about 22 claim 24 ,000 cells/cmwhen the pre-coated substrate is contacted with 1 ml of a suspension of cells from the SiHa cell line.26. The pre-coated substrate according to claim 24 , wherein the pre-coated substrate surface is capable of immobilizing an average number of cells per surface area of at least about 23 claim 24 ,000 cells/cmwhen the pre-coated substrate is contacted with 1 ml of a suspension of cells from the SiHa cell line.27. The pre-coated substrate according to claim 24 , wherein the substrate is selected from the group consisting of slides claim 24 , ...

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Номер записи: 46
10-04-2014 дата публикации

SOLID PHASE EXTRACTION DISK HOLDER APPARATUS

Номер: US20140097139A1
Автор: JOHNSON Robert S.
Принадлежит:

Solid phase extraction (SPE) disks provide the greatest extraction efficiency when the maximum surface area of the disk is exposed to the sample. Such may be accomplished with the present disclosure wherein an SPE disk is secured within a cavity of a disk holder apparatus such that the SPE disk compresses against a side wall defining the cavity and forms a seal therebetween. The disk holder apparatus may be used in an open design for vacuum applications, or in a sealed design with a closure, when using positive pressure to force the sample through the disk. Examples of various configurations for the disk holder apparatus and methods of use are provided. 1. A solid phase extraction system comprising:a solid phase extraction disk having a thickness;a solid phase extraction disk holder apparatus;wherein the solid phase extraction disk holder apparatus comprises a basin having a cavity to receive the solid phase extraction disk, the cavity defined by a bottom wall and an adjoining side wall of the basin; andwherein the solid phase extraction disk, when in the cavity, compresses against the side wall of the basin.2. The solid phase extraction system of claim 1 , wherein:the solid phase extraction disk forms a seal with the side wall of the basin.3. The solid phase extraction system of claim 1 , wherein:the cavity of the basin forms a reservoir to contain a fluid sample which is to flow through the solid phase extraction disk; andthe seal inhibits the fluid sample from flowing between the solid phase extraction disk and the side wall of the basin.4. The solid phase extraction system of wherein:the bottom wall of the basin includes a fluid outlet for the fluid sample.5. The solid phase extraction system of further comprising:a screen located between the solid phase extraction disk and the bottom wall of the cavity.6. The solid phase extraction system of wherein:the basin includes a recess in the bottom wall to receive the screen7. The solid phase extraction system of ...

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Номер записи: 47
01-01-2015 дата публикации

AUTOMATIC SAMPLE POURING DEVICE

Номер: US20150000386A1
Автор: Minato Hiroyuki
Принадлежит: SHIMADZU CORPORATION

An automatic sample pouring device comprising a rack, a sampling needle, a reference portion arranged at a predetermined position of the rack, a detection unit for electrically detecting contact between the reference portion and the needle, and a control unit for controlling an operation of relatively moving the needle on a horizontal plane and in a vertical direction with respect to the rack, and suctioning and pouring operations of a sample. The control unit is configured to control an operation of obtaining reference position information of the rack on the basis of detection information of the detection unit. 15.-. (canceled)6. An automatic sample pouring device comprising:a rack allowing mounting of a plurality of sample containers;a sampling needle for suctioning samples contained in the sample containers;a reference portion, arranged at a predetermined position of the rack, including a concave portion of a predetermined depth, a shape of an inner wall surface of the reference portion in horizontal cross section being point-symmetric;a detection unit for electrically detecting contact between the reference portion and the needle; anda control unit for controlling an operation of relatively moving the needle on a horizontal plane and in a vertical direction with respect to the rack, an operation of obtaining reference position information of the rack on the basis of detection information of the detection unit, and suctioning and pouring operations of a sample.7. The automatic sample pouring device according to claim 6 ,wherein, after moving a tip end of the needle into the reference portion, the control unit moves, until contact between the reference portion and the needle is detected on the basis of detection information of the detection unit, the needle in both positive and negative directions in an X direction on the horizontal plane, both positive and negative directions in a Y direction that is orthogonal to the X direction on the horizontal plane, and a Z ...

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Номер записи: 48
07-01-2016 дата публикации

SOLUTE EXTRACTING APPARATUS

Номер: US20160003784A1
Автор: FUJITA Hiroyuki, Nakamura Hirofumi
Принадлежит: MIURA CO., LTD.

A solute trapping column including two trapping agent layers has a first branched pathway extending from an upper part of a main body part thereof, and a second branched pathway extending from between two trapping agent layers, and with its upper part a purification column is connected. By injection of solution of dioxins and supply of solvent into the purification column, dioxins are trapped by the trapping agent layers. An extraction solvent supplied from the lower end side of the solute trapping column while keeping both the purification column and the first branched pathway blocked extracts the dioxins trapped by the second trapping agent layer and flows into the second branched pathway. An extraction solvent supplied in a similar manner while keeping both the purification column and the second branched pathway blocked extracts the dioxins trapped by the first trapping agent layer and flows into the first branched pathway. 1. An apparatus for extracting a solute from a solution , comprising:a solute trapping column having an injection part equipped with an injection port for the solution at one end and a discharge port at the other end, and including multiple trapping agent layers capable of trapping the solute packed with a space interposed therebetween;a first supplying device for supplying the solute trapping column with a solvent for developing the solute within the solute trapping column; anda second supplying device for supplying the solute trapping column with a solvent for extracting the solute,wherein, the solute trapping column has branched pathways, which are independent of each other and respectively extend from the injection part and the space, for the flow of the extraction solvent from the second supplying device, andthe second supplying device is settable during its operation so that the extraction solvent flows in one of the branched pathways sequentially selected from the extraction solvent supply side while flowing of the extraction solvent in ...

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Номер записи: 49
02-01-2020 дата публикации

Dexrazoxane analytical method

Номер: US20200003737A1
Автор: Changjun FAN, Hongyu Chen, Min Sun, Shuaihua TIAN, Xiangfeng CHEN
Принадлежит: Jiangsu Aosaikang Pharmaceutical Co Ltd

A high performance liquid chromatography method used for dexrazoxane-related substances is provided, and in the method, a low-density bonding reversed-phase C18 chromatographic column resistant to pure water is employed; a gradient elution is carried out with mobile phase A and mobile phase B as eluents, the mobile phase A being a buffer, and the mobile phase B being an organic solvent; the volume percent of mobile phase A in eluents in a first stage of the gradient elution is not lower than 90%, and the duration of the first stage of the gradient elution ranges from 15˜30 minutes. By means of the analytical method, dexrazoxane is effectively separated from main impurities, and the qualities of the active pharmaceutical ingredients of dexrazoxane and the preparations thereof could be better controlled.

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Номер записи: 50
07-01-2021 дата публикации

ANALYSIS METHOD OF 3-METHYL-1-PHENYL-2-PYRAZOLIN-5-ONE BULK DRUG, TREATMENT FOR AMYOTROPHIC LATERAL SCLEROSIS, INHIBITION OF PROGRESSION OF AMYOTROPHIC LATERAL SCLEROSIS, AND METHOD OF PRODUCING DRUG CONTAINING 3-METHYL-1-PHENYL-2-PYRAZOLIN-5-ONE BULK DRUG

Номер: US20210003544A1
Автор: SANO Aya, WAKASUGI Takeshi
Принадлежит: MITSUBISHI TANABE PHARMA CORPORATION

This method of analyzing the phenylhydrazine content in a 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug involves: obtaining a first measured value by measuring the phenylhydrazine content of a standard solution that contains phenylhydrazine or a salt thereof, a first acidic water and a first water-soluble organic solvent, and that exhibits a phenylhydrazine concentration of 0.01 μg/mL to 10 μg/mL; obtaining a second measured value by measuring the phenylhydrazine content in a sample solution that contains a 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug, a second acidic water and a second water-soluble organic solvent; and detecting the phenylhydrazine content in the 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug on the basis of the first measured value and the second measured value. The first acidic water is at least one type selected from hydrochloric acid, an aqueous acetic acid solution, and the like, the first water-soluble organic solvent is at least one type selected from acetonitrile and methanol, the second acidic water is at least one type selected from hydrochloric acid, an aqueous acetic acid solution, and the like, and the second water-soluble organic solvent is at least one type selected from acetonitrile, and methanol. 1. A method of analyzing the phenylhydrazine content in a 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug , which comprises:obtaining a first measured value by measuring the phenylhydrazine content in a phenylhydrazine standard solution that contains phenylhydrazine or a salt thereof, a first acidic water and a first water-soluble organic solvent, and that exhibits a phenylhydrazine concentration of 0.01 μg/mL to 10 μg/mL,obtaining a second measured value by measuring the phenylhydrazine content in a 3-methyl-1-phenyl-2-pyrazolin-5-one sample solution that contains a 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug, a second acidic water and a second water-soluble organic solvent, anddetecting the phenylhydrazine content in the 3-methyl-1- ...

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Номер записи: 51
07-01-2021 дата публикации

Method for improving monoclonal antibody detection results

Номер: US20210003587A1
Автор: Noriko Iwamoto, Takashi Shimada
Принадлежит: Shimadzu Corp

The present invention provides a detection method for a monoclonal antibody in a sample, comprising: (a) a step of capturing the monoclonal antibody in the sample and immobilizing the monoclonal antibody in pores of a porous body; (b) a step of bringing the porous body in which the monoclonal antibody is immobilized with nanoparticles on which protease is immobilized to conduct selective protease digestion of the monoclonal antibody; (c) a step of detecting, by a liquid chromatography mass spectrometry (LC-MS), peptide fragments obtained by the selective protease digestion; and (a′) a step of conducting a reduction reaction under an acidic condition after the step (a). By the present invention, further applicability of the detection method for the monoclonal antibodies using mass spectrometry is expected.

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Номер записи: 52
03-01-2019 дата публикации

SYSTEM AND METHOD FOR DESORBING AND DETECTING AN ANALYTE SORBED ON A SOLID PHASE MICROEXTRACTION DEVICE

Номер: US20190003936A1
Автор: Pawliszyn Janusz, Singh Varoon, Tascon Marcos
Принадлежит:

Disclosed herein is a system for desorbing and detecting an analyte sorbed on a solid phase microextraction (SPME) device. This SPME device The system includes a desorption chamber containing solvent required for desorption of analytes from SPME device; a flow injector in fluid connection with the desorption chamber, the desorption chamber and the flow injector being fluidly connected by at least a flow-insulating fluid connector; a solvent source in fluid connection with the flow injector; and a fluid switch that: in a desorption position, allows the solvent to be sprayed from the flow injector while flow-insulating any desorption solution in the desorption chamber, and in an detecting position, turns off the solvent source while maintaining the fluid connection between the flow injector and the desorption chamber, transferring the desorption solution through the flow-insulating fluid connector to the flow injector as a substantially undiluted plug of liquid. The SPME device can be configured to be various morphologies such as, fibers, blades, thin film membranes and even magnetic particles. When magnetic particles are used an additional holder that contains an embedded magnet which holds a plate with a well to hold said magnetic particles is added to the system. 1. A system for desorbing and detecting an analyte sorbed on a solid phase microextraction (SPME) device , the system comprising:a desorption chamber containing the desorption fluid forming a dome in contact with said SPME device;a flow injector in fluid connection with the desorption chamber, the desorption chamber and the flow injector being fluidly connected by at least a flow-insulating fluid connector;a solvent source in fluid connection with the flow injector; and (a) in a desorption position, allows the solvent to be sprayed from the flow injector while flow-insulating any desorption solution in the desorption chamber, and', '(b) in an detecting position, isolates the solvent source from the flow ...

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Номер записи: 53
20-01-2022 дата публикации

DYNAMIC HEADSPACE VACUUM TRANSFER "IN TRAP" EXTRACTION METHOD AND APPARATUS

Номер: US20220018740A1
Автор: BISCHOFF Patrick, FUCHSMANN Pascal, TENA STERN Mireille, WAHL Fabian
Принадлежит:

A headspace extraction and analysis method for volatile compounds can be performed in automatic mode in a HS-ITEX apparatus adapted to be additionally connectable to a vacuum source. The method includes extracting the headspace by sucking volatile compounds into a sorbent-containing trap followed by desorption into the injector of a GC-MS analyzer. 1. A headspace extraction and analysis method for volatile compounds , wherein a continuous flow of volatile compounds is forced by applying reduced pressure conditions , comprising(i) inserting a needle of a sampling means via a septum into a headspace above a solid or liquid sample contained in a container closed by said septum, wherein said sampling means has a trap filled with at least one sorbent, said trap has a first end and a second end, at the first end the trap is connected to the needle, and at the second end the trap is connected with at least one flow channel configured to connect the trap to a vacuum source and an inert gas source,(ii) applying a vacuum to the sampling means on the side of the second end of the trap, so that volatile compounds in the headspace are sucked out from the container into the trap,(iii) removing the sampling means from the container,(iv) drying the sampling means by flowing a dry inert gas through the sampling means from the second side,(v) inserting the needle into an injector of a gas chromatography apparatus,(vi) desorbing the volatile compounds from the trap by flowing inert gas through the at least one sorbent from the second side,(vii) analyzing the volatile compounds and(viii) cleaning the trap and needle by flushing with inert gas.2. The method of claim 1 , wherein the temperature of the trap in steps (i) to (iii) is lower than the temperature of the trap in steps (vi) and (viii).3. The method of claim 1 , wherein:the sampling means has a body forming said at least one flow channel,in step (ii), the at least one flow channel is used for applying the vacuum, andin the steps ...

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Номер записи: 54
08-01-2015 дата публикации

INTRODUCING AN ANALYTE INTO A CHEMICAL ANALYZER

Номер: US20150010442A1
Автор: Bowden Thomas, Ojeda Pedro, Rafferty David, Spencer Michael, Wylde James
Принадлежит:

A chemical pre-concentrator includes a conduit defining a flow path between two ends and having a heating element disposed within the conduit, such that the heating element has at least one sorbent material deposited directly on at least a portion of a conductive surface of the heating element. Some such heating elements are in the form of electrically conductive strips defining both a plurality of apertures through the strip and a series of undulations spaced along the flow path. 1. A chemical pre-concentrator comprising:a conduit defining a flow path between two ends; anda heating element disposed within the conduit;wherein the heating element has an electrically conductive surface and at least one sorbent material deposited directly on at least a portion of the surface.233-. (canceled) This application claims the benefit of priority to U.S. Provisional Application Ser. No. 61/440,267, filed on Feb. 7, 2011, the entire contents of which are incorporated by reference herein.This specification relates to introducing an analyte into a chemical analyzer for analysis.Chemical analysis took such as gas chromatography (“GC”), mass spectrometers (“MS”), ion mobility spectrometers (“IMS”), and various others, are commonly used to identify trace amounts of chemicals, including, for example, chemical warfare agents, explosives, narcotics, toxic industrial chemicals, volatile organic compounds, semi-volatile organic compounds, hydrocarbons, airborne contaminants, herbicides, pesticides, and various other hazardous contaminant emissions. A summary of available detection technologies is contained is Yin Sun and Kowk Y Ong, Detection Technologies for Chemical Warfare Agents and Toxic Vapors, 2005, CRC Press, ISBN 1-56670-668-8 (“Sun & Ong”).Chemical detectors have a minimum concentration of analyte in a matrix that can be detected. For some chemicals, particularly threats, it is desirable to detect at extremely low concentrations compared to the sensitivity limit of typical ...

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Номер записи: 55
27-01-2022 дата публикации

QUANTIFICATION OF SURFACE ACIDITY ON A LOW SURFACE AREA MATERIAL

Номер: US20220026322A1
Автор: Ralph James M, Siengchum Tritti
Принадлежит: SAINT-GOBAIN CERAMICS & PLASTICS, INC.

Methods and systems of analyzing and quantifying a surface acidity on a low surface area sample are disclosed. The methods include analyzing a quantity of an effluent molecular probe desorption from a temperature programmed desorption (TPD) process and system, by employing a mass spectroscopy (MS) system. The molecular probe is chemically adsorbed on a clean surface of the sample before the TPD process. The sample has a surface area to mass ratio of less than 5 m/g. In some embodiments, the total surface area analyzed in the method can be as low as 3 m. 1. A method of quantification of surface acidity of a sample , the method comprising the steps of:(1) removing surface moisture of the sample using a pretreatment process;(2) adsorbing a molecular probe on a surface of the sample;(3) removing physically adsorbed molecular probe from the surface of the sample;(4) releasing the adsorbed molecular probe by a method that includes a temperature programmed desorption (TPD) process; and(5) analyzing a total amount of the molecular probe desorbed from the TPD process by a method that includes mass spectroscopy (MS),{'sup': '2', 'wherein the sample has a surface area to mass ratio of less than 5 m/g.'}2. The method of claim 1 , wherein the surface area to mass ratio is less than 1 m/g.3. The method of claim 1 , wherein the sample has a total surface area of at least 3 m.4. The method of claim 3 , wherein the sample has a total surface area of not greater than 25 m.5. The method of claim 1 , wherein the sample comprises a catalyst carrier.6. The method of claim 1 , wherein the sample comprises a ceramic material.7. The method of claim 1 , wherein the sample comprises alumina claim 1 , zirconia claim 1 , titania claim 1 , silica claim 1 , zeolite claim 1 , or a combination thereof.8. The method of claim 1 , further comprising: calculating a surface acidity site density based on the total amount of the molecular probe desorbed and a total surface area of the sample.9. The method ...

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Номер записи: 56
27-01-2022 дата публикации

GAS CHROMATOGRAPH, MAINTENANCE SWITCH MODE SETTING METHOD AND NON-TRANSITORY COMPUTER READABLE MEDIUM STORING MAINTENANCE SWITCH MODE SETTING PROGRAM

Номер: US20220026402A1
Автор: KOMORI Yuki
Принадлежит: SHIMADZU CORPORATION

A gas chromatograph includes first and second sample vaporization units, first and second separation columns, first and second carrier gas suppliers, a column oven that contains the first and second separation columns and has a first heater, first and second detectors, a first auxiliary gas supplier that supplies an auxiliary gas to the first separation column and a controller that executes control by increasing a supply pressure of an auxiliary gas applied by the first auxiliary gas supplier to a pressure higher than a pressure during an analysis of a sample and lowering a supply pressure of a carrier gas applied by the first carrier gas supplier to a pressure lower than a pressure during the analysis of the sample such that the supply pressure of the auxiliary gas is higher than the supply pressure of the carrier gas, and sets a first maintenance switch mode in which the first sample vaporization unit is maintainable. 1. A gas chromatograph comprising:a first sample vaporization unit that vaporizes a sample;a second sample vaporization unit that vaporizes a sample;a first separation column that separates a sample supplied from the first sample vaporization unit into respective sample components;a second separation column that separates a sample supplied from the second sample vaporization unit into respective sample components;a first carrier gas supplier that supplies a carrier gas, for guiding a sample that is vaporized in the first sample vaporization unit to the first separation column, to the first sample vaporization unit;a second carrier gas supplier that supplies a carrier gas, for guiding a sample that is vaporized in the second sample vaporization unit to the second separation column, to the second sample vaporization unit;a column oven that contains the first and second separation columns and has a first heater for heating the first and second separation columns;a first detector that detects respective sample components obtained by separation in the ...

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Номер записи: 57
11-01-2018 дата публикации

GAS CHROMATOGRAPH

Номер: US20180011066A1
Автор: ISZ Sandrine, LOUBET François, Mifsud Jean-Christophe, PELLETIER Sébastien
Принадлежит: ALPHA M.O.S

A hybrid device comprising Metal Oxide Sensors in a Gas Chromatography column is described, whereby the readings from the MOS devices will vary along the column in reaction to the sample reflecting the differential delays imposed on the components of the sample depending on the elutive effect of the adsorbent lining the column for the respective component. By this means, a family of readings is obtained, any one of which may be easier to interpret for a particular sample, and which may be compared amongst themselves providing an additional measurement dimension. The behaviour of later sections of column or sensors may be modified dynamically during a measurement cycle depending on the readings obtained at the earlier stages. 1. A device for characterizing a gas , said device comprising:a conduit containing one or more regions of a first adsorbent material distributed along its length;a first gas sensor at a proximal extremity of said conduit situated so as to detect at least a first molecule in said conduit;a second gas sensor at a distal extremity of said conduit situated so as to detect at least a second molecule in said conduit; andan inlet for introduction of said gas at said proximal end of said conduit, wherein said conduit, said first gas sensor an said second gas sensor are so disposed that a gas sample being introduced at said inlet proceeds through said first gas sensor, said conduit and said second gas sensor in sequence.2. The device of wherein said first gas sensor and said second gas sensor are Metal Oxide Sensor devices.3. The device of wherein said first molecule and said second molecule are the same.4. The device of comprising a second conduit between said inlet and said first gas sensor claim 1 , said second conduit containing one or more regions of a second adsorbent material distributed along its length.5. The device of wherein said first adsorbent material and said second adsorbent material are the same.6. The device of comprising a further ...

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Номер записи: 58
03-02-2022 дата публикации

QUANTITATIVE DETECTION METHOD FOR SNAKE VENOM THROMBIN-LIKE ENZYME (SVTLE)

Номер: US20220033873A1
Автор: Chi Lianli, Du Hongming, Gong Liping, Hang Baojian, Shi Feng, WANG Congcong, Wang Weijian, Xian Ruiqing, You Pengfei
Принадлежит:

The present invention relates to the technical field of chemical analysis and quantitative detection, in particular to a quantitative detection method for snake venom thrombin-like enzyme (SVTLE) from . The quantitative detection method for the SVTLE includes the following steps of taking a reference substance of marker peptide for the SVTLE from with an amino acid sequence of LDSPVSNSAHIAPLSLPSSAPSVGSVCR, and preparing a series of reference solutions with different concentrations; adding the reference solutions in test solutions respectively for enzymolysis, and then taking a supernatant after enzymolysis as a series of solutions to be detected; and adding the solutions to be detected in a liquid chromatogram-mass spectrometer, and then selecting a qualitative ion pair and a quantitative ion pair to detect contents of marker peptide in the solutions to be detected. 1. A quantitative detection method for snake venom thrombin-like enzyme (SVTLE) , comprising the following specific steps of:{'i': 'Agkistrodon halys pallas', '(1) taking a reference substance of a marker peptide for SVTLE from with an amino acid sequence of LDSPVSNSAHIAPLSLPSSAPSVGSVCR, and dissolving and diluting the reference substance to prepare a series of reference solutions with different concentrations;'}(2) taking a to-be-detected sample, and dissolving the to-be-detected sample to prepare a test solution;(3) taking the series of reference solutions with different concentrations in step (1), adding the test solutions in step (2) respectively for uniform mixing, conducting enzymolysis with trypsin, and then taking supernatants after enzymolysis as a series of solutions to be detected;(4) injecting the solutions to be detected in step (3) into a liquid chromatogram-mass spectrometer respectively, conducting multiple-reaction monitoring by adopting an electrospray positive ion mode, and conducting detection with tricharged 935.8→602.3 as a qualitative ion pair and tricharged 935.8→861.4 as a ...

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Номер записи: 59
03-02-2022 дата публикации

ANALYTICAL DEVICE

Номер: US20220034771A1
Автор: MIYAMOTO Ayaka, WADA Toyohito
Принадлежит: SHIMADZU CORPORATION

An analytical device includes: a sample vaporization chamber into which a sample is to be injected and which, if the sample is a liquid sample, is configured to vaporize the liquid sample; a first temperature regulation unit and a second temperature regulation unit configured to regulate a temperature of the sample vaporization chamber; and a detection unit configured to detect the sample which is separated at a separation column that is connected with the sample vaporization chamber, the separation column being configured to separate the sample in a gas phase. 1. An analytical device comprising:a sample vaporization chamber into which a sample is to be injected and which, if the sample is a liquid sample, is configured to vaporize the liquid sample;a first temperature regulation unit and a second temperature regulation unit configured to regulate a temperature of the sample vaporization chamber; anda detection unit configured to detect the sample which is separated at a separation column that is connected with the sample vaporization chamber, the separation column being configured to separate the sample in a gas phase.2. The analytical device according to claim 1 , wherein:the first temperature regulation unit is configured to regulate the temperature of a first part of the sample vaporization chamber; andthe second temperature regulation unit is configured to regulate the temperature of a second part of the sample vaporization chamber which is different from the first part of the sample vaporization chamber.3. The analytical device according to claim 2 , wherein:the first temperature regulation unit is capable of performing regulation so as to at least one of increase or decrease the temperature of the first part of the sample vaporization chamber; andthe first part of the sample vaporization chamber is farther from the separation column than the second part of the sample vaporization chamber.4. The analytical device according to claim 3 , wherein:the second ...

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Номер записи: 60
03-02-2022 дата публикации

SNAKE VENOM THROMBIN-LIKE ENZYME MARKER PEPTIDE OF AGKISTRODON HALYS PALLAS AND ITS APPLICATION IN THE SPECIES IDENTIFICATION OF HEMOCOAGULASE FOR INJECTION

Номер: US20220034853A1
Автор: Chi Lianli, Du Hongming, Gong Liping, Hang Baojian, Shi Feng, WANG Congcong, Wang Fengshan, Wang Weijian, Xian Ruiqing, Zhang Qunye
Принадлежит:

The present invention provides a snake venom thrombin marker peptide of and an application of the snake venom thrombin-like enzyme in identifying species of Hemocoagulase for Injection. The application includes the following steps of: dissolving a to-be-detected sample and a reference substance of the marker peptide respectively to prepare a test solution and a reference solution, and conducting alkylation reduction on the test solution and the reference solution with dithiothreitol and iodoacetamide; after diluting products with an ammonium bicarbonate solution, adding enzyme for hydrolysis; and after enzymolysis is finished, conducting centrifugation at a high speed, and injecting a supernatan into a liquid chromatography-mass spectrometer for analysis. This method is simple, convenient and rapid, is strong in specificity, fills the gap in identifying the source of species of the snake venom thrombin-like enzyme of and improves the quality control level. 1Agkistrodon Halys PallasAgkistrodon Halys Pallas. A snake venom thrombin marker peptide of , wherein an amino acid sequence of the snake venom thrombin marker peptide of is LDSPVSNSAHIAPLSLPSSAPSVGSVCR.2Agkistrodon Halys Pallas. An method of using the snake venom thrombin marker peptide of according to in identifying species of snake venom thrombin for injection.3Agkistrodon Halys Pallas. The method according to claim 2 , wherein the method of using the snake venom thrombin marker peptide of in identifying the species of snake venom thrombin for injection comprises the following steps of:{'i': 'Agkistrodon Halys Pallas', 'A: dissolving the snake venom thrombin for injection and the snake venom thrombin marker peptide of into a test solution and a reference solution respectively;'}B: conducting an alkylation reduction treatment on the test solution and the reference solution with dithiothreitol and iodoacetamide;C: after the alkylation reduction treatment is finished, adding enzyme for a hydrolysis;{'i': ...

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Номер записи: 61
16-01-2020 дата публикации

GAS CHROMATOGRAPH

Номер: US20200018732A1
Автор: KOMORI Yuki, TAKECHI Ryo
Принадлежит: SHIMADZU CORPORATION

A gas chromatograph includes a sample introduction portion and a heater block. A contact portion is fit into a first recess of a main body of the sample introduction portion. The heater block is fixed to and positioned with respect to the main body of the sample introduction portion by directly coming into contact with the contact portion provided on the main body of the sample introduction portion. For this reason, the heater block may be surely positioned with respect to the sample introduction portion. In addition, the heater block may be positioned without providing a member such that heat from the heater block is transferred to the outside and lost, and thus the sample introduction portion may be efficiently heated. 1. A gas chromatograph comprising:a sample introduction portion in which a sample vaporization chamber for vaporizing a sample is formed;a heater block that heats the sample introduction portion from an outside; anda positioning mechanism that positions the heater block with respect to the sample introduction portion by bringing the heater block into contact with a contact portion provided on the sample introduction portion.2. The gas chromatograph according to claim 1 , wherein the heater block is a tubular member claim 1 ,the sample introduction portion is inserted into the heater block along an axis direction, andthe positioning mechanism positions the heater block with respect to the sample introduction portion while interposing both end portions of the heater block in the axis direction.3. The gas chromatograph according to claim 1 , wherein the positioning mechanism is allowed to change a relative position of positioning of the heater block with respect to the sample introduction portion.4. The gas chromatograph according to claim 3 , 'the positioning mechanism is allowed to change the relative position of positioning of the heater block with respect to the sample introduction portion by bringing the heater block into contact with the contact ...

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Номер записи: 62
16-01-2020 дата публикации

SECONDARY ULTRASONIC NEBULISATION

Номер: US20200018733A1
Автор: Bajic Stevan, Douce David S., Jones Gordon A.
Принадлежит: MICROMASS UK LIMITED

A secondary ultrasonic nebulisation device is disclosed comprising: a liquid sample delivery capillary; a sample receiving surface arranged for receiving a liquid sample from the capillary; and an ultrasonic transducer configured for oscillating the surface so as to nebulise the liquid sample received thereon, wherein the device is configured such that the oscillations of the surface by the ultrasonic transducer cause charged droplets and/or gas phase ions to be generated from the sample. 120-. (canceled)21. A mass or ion mobility spectrometer comprising: a liquid sample delivery capillary;', 'a sample receiving surface arranged for receiving a liquid sample from the capillary; and', 'an ultrasonic transducer configured for oscillating the surface so as to nebulise the liquid sample received thereon, wherein the device is configured such that the oscillations of the surface by the ultrasonic transducer cause charged droplets and/or gas phase ions to be generated from the sample;, 'a secondary ultrasonic nebulisation device comprisinga vacuum chamber;an ion inlet orifice arranged between the sample receiving surface of the nebulisation device and the vacuum chamber, for receiving said charged droplets and/or ions;a structural component arranged in a flow path of the nebulised droplets and/or ion; anda heater configured to heat the structural component.22. The mass or ion mobility spectrometer of claim 21 , wherein the structural component is arranged in the vacuum chamber.23. The mass or ion mobility spectrometer of claim 22 , wherein the structural component is arranged in the flow path of the charged droplets and/or ions such that claim 22 , in use claim 22 , the droplets and/or ions impact onto the structural component.24. The mass or ion mobility spectrometer of claim 22 , wherein the structural component comprises a heated bead.25. The mass or ion mobility spectrometer of claim 22 , wherein the heater heats the structural component such that claim 22 , in use ...

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Номер записи: 63
21-01-2021 дата публикации

ASYMMETRIC FLOW FIELD FLOW FRACTIONATION APPARATUS

Номер: US20210018474A1
Автор: YAMADA Hironobu
Принадлежит:

An asymmetric flow field flow fractionation apparatus is provided with a separation cell having a semi-permeable membrane. Each flow path is connected to the separation cell. A mobile phase that has passed through the semi-permeable membrane of the separation cell flows through a crossflow discharge flow path. The crossflow discharge flow path is provided with a flow controller for adjusting the flow rate. A voltage control unit applies a voltage according to a set flow rate to the flow controller when the set flow rate of the mobile phase of the crossflow discharge flow path is constant, and applies a voltage in which an offset is added to the voltage according to the set flow rate to the flow controller to the flow controller when the set flow rate of the crossflow discharge flow path mobile phase increases. Therefore, even if the set flow rate is changed so as to be increased, the flow rate of the mobile phase moving in the crossflow discharge flow path can be changed so as to correspond to the set flow rate. 1. An asymmetric flow field flow fractionation apparatus comprising:a separation cell provided with a semi-permeable membrane and configured to classify particles in a liquid sample by applying a flow field to a liquid sample diluted with a mobile phase;a detector configured to detect the particles in the liquid sample classified by the separation cell;a first outflow path configured to connect the separation cell and the detector;a second outflow path configured to flow out the mobile phase in the separation cell through the semi-permeable membrane;a flow controller configured to control a flow rate of the mobile phase flowing through the second outflow path; anda control unit configured to control an applied voltage to the flow controller,wherein when a set flow rate of the mobile phase in the second outflow path is constant, the control unit applies a voltage corresponding to the set flow rate to the flow controller, and when the set flow rate of the ...

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Номер записи: 64
22-01-2015 дата публикации

CHROMATOGRAPHIC ISOLATION OF CELLS AND OTHER COMPLEX BIOLOGICAL MATERIALS

Номер: US20150024411A1
Автор: STADLER Herbert
Принадлежит:

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials. 1. A method of isolating a target cell , wherein the target cell has a receptor molecule on the target cell surface , the method comprising:providing a sample, the sample comprising the target cell,providing a receptor binding reagent comprising a binding site B and a binding partner C,{'sub': 'D', 'sup': −5', '−1, 'wherein the binding site B comprised in the receptor binding reagent is capable of specifically binding to the receptor molecule on the target cell surface, wherein the dissociation constant (K) for the binding between the receptor binding reagent via the binding site B and the receptor molecule is of low affinity or wherein the dissociation rate constant (koff) for the binding between the receptor binding reagent via the binding site B and the receptor molecule has a value of about 3×10secor greater, wherein the binding partner C comprised in the receptor binding reagent is capable of reversibly binding to a binding site Z of an affinity reagent, and'}exposing the sample to chromatography on a suitable stationary phase, the stationary phase having the affinity reagent immobilized thereon,wherein the affinity reagent comprises a binding site Z, wherein said binding site Z forms a reversible bond with the binding partner C comprised in the receptor binding reagent, and wherein the binding site B of the receptor binding reagent binds to a receptor ...

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Номер записи: 65
28-01-2016 дата публикации

CHEMICAL SENSING DEVICE

Номер: US20160025605A1
Автор: Burgess James, Krasnobaev Leonid, Lahann Joerg, Lawrence Tyson, MAHMUD Ken, WELING Aniruddha
Принадлежит:

A chemical sensing system includes a substrate material, a detector capable of indicating a presence of a target compound, gas, or vapor, and a heater for rapidly releasing compounds, gases and vapors from the substrate material. The substrate material acts to concentrate the compounds, gases, and vapors from a sample area for improved detection by the detector. 1. A preconcentrator comprising a cartridge and a substrate coated with an organic compound , wherein the organic compound comprises copolymers having polar functional groups and hydrophobic functional groups , and wherein the substrate is enclosed within the cartridge.2. The preconcentrator of claim 1 , wherein the substrate is selected from the group consisting of metal fiber claim 1 , woven metal fibers claim 1 , non-woven metal fibers claim 1 , porous metal claim 1 , sheet metal claim 1 , metal coated glass claim 1 , metal coated plastic claim 1 , metal coated ceramic claim 1 , carbonaceous material claim 1 , graphite claim 1 , charcoal claim 1 , activated carbon claim 1 , activated carbon cloth claim 1 , and combinations thereof.3. The preconcentrator of claim 1 , wherein the substrate has a resistivity of about 10ohm-meters (Ω·m) to about 10Ω·m claim 1 , magnetic permeability of greater than about 1×10H/m claim 1 , relative permeability of greater than 100 claim 1 , or combinations thereof.4. The preconcentrator of claim 1 , wherein the organic compound has an electrical resistivity of greater than 10Ω·m.5. The preconcentrator of claim 1 , wherein the polar functional groups are selected from the group consisting of amide (—C(O)NH) claim 1 , C-Calkyl amide claim 1 , carboxylic acid (—COOH) claim 1 , C-Calkyl carboxylic acid claim 1 , hydroxyl (—OH) claim 1 , C-Calkyl hydroxyl claim 1 , C-Calkyoxy claim 1 , aldehyde (—C(O)H) claim 1 , C-Calkyl aldehyde claim 1 , ketone (—C(O)CH) claim 1 , C-Calkyl ketone claim 1 , amine (—NH) claim 1 , C-Calkyl amine claim 1 , and combinations thereof.6. The ...

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Номер записи: 66
26-01-2017 дата публикации

COOLING-ASSISTED NEEDLE TRAP DEVICE FOR ANALYZING COMPLEX SOLID SAMPLES USING NANO-SORBENT

Номер: US20170023533A1
Автор: Dowlatshah Samira, Ghiasvand Alireza, Heidari Nahid
Принадлежит:

A cooling-assisted needle trap device for sampling and delivering materials to an analytical device is disclosed. The device includes a needle having a first end and a second end and a side aperture located between the first and second ends. The side aperture provides access to the interior space of the needle. A sorbent is packed within an interior space of the needle between the second end and the side aperture to entrap an analyte within a sample. The cooling-assisted needle trap device also includes a cooling device configured to cool the sorbent. 1. A cooling-assisted needle trap system comprising:a syringe;a needle including a first end and a second end and a side aperture positioned between the first end and the second end, the first end being coupled to the syringe, and the side aperture being configured to provide access to the interior space of the needle;a nano-composite sorbent placed between the second end and the side aperture within the interior space of the needle and configured to entrap an analyte within a sample received within the interior space of the needle; anda cooling device configured to cover and cool the sorbent and includes an inner tube and an outer tube, the outer tube having a diameter larger than the inner tube and including a first aperture and a second aperture;a first capillary tube coupled to the first aperture and configured to be an inlet channel for a coolant fluid for cooling the sorbent; anda second capillary tube coupled to the second aperture and configured to be an outlet channel for the coolant fluid.2. The system of claim 1 , wherein:the inner tube and the outer tube include substantially equal length,the first aperture is included near one end of the outer tube,the second aperture is included near another end of the outer tube,the first capillary tube coupled to the first aperture has a larger diameter than the second capillary tube coupled to the second aperture to enhance an efficiency of the cooling device, andthe ...

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Номер записи: 67
10-02-2022 дата публикации

COMPOSITIONS AND METHODS FOR REMOVING INTERFERING SUBSTANCES

Номер: US20220042886A1
Автор: Lee Huey, Son Michelle, Vitzthum Frank
Принадлежит: SIEMENS HEALTHCARE DIAGNOSTICS INC.

Disclosed herein are compositions and methods for removing interfering substances from biological samples, biochemical assays, or reagents used in biological samples using porous liposomes that capture and in some instances enrich said interfering substances within the liposomes. 1. A composition comprising a liposome , the liposome comprising an interior , a lipid bilayer , and a pore-forming substance in the lipid bilayer.2. The composition of claim 1 , wherein the pore-forming substance forms pores in the lipid bilayer.3. The composition of claim 1 , wherein the pore-forming substance comprises a pore-forming protein or protein complex comprising porin claim 1 , hemolysin claim 1 , nucleoporin claim 1 , membrane attack complex of the complement system claim 1 , complement C9 multimer claim 1 , or combinations thereof.4. The composition of claim 1 , comprising about 1 μg/ml to about 100 μg/ml of the pore-forming substance claim 1 , preferably about 20 μg/ml.5. The composition of claim 2 , wherein the pores allow passage of one or more interferents into the liposome via diffusion.6. The composition of claim 2 , wherein the pores have an inner diameter of about 0.5 nanometers to about 12 nanometers or an exclusion limit of about 250 to about 5000 Da.7. The composition of claim 1 , wherein a binder is disposed in the liposome.8. The composition of wherein the binder has a maximum cross-section that is greater than the inner diameter of the pores or the binder is larger than the exclusion limit of the pores.9. The composition of claim 8 , wherein at least a portion of the binder is disposed in the lipid bilayer.10. The composition of claim 8 , wherein the binder is disposed entirely within the interior of the liposome.11. The composition of claim 7 , wherein the binder comprises one or more binding sites claim 7 , each binding site having an affinity for one or more interferents.12. The composition of claim 7 , wherein the binder comprises avidin claim 7 , ...

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Номер записи: 68
10-02-2022 дата публикации

Peak Profile for Identifying an Analyte in a Chromatogram

Номер: US20220042957A1
Автор: DASGUPTA Purnendu K., Kadjo Akinde F., Srinivasan Kannan
Принадлежит:

A method of determining an identity of a first analyte in a sample is described that includes passing the first analyte through a chromatographic column and detecting a signal curve of the first analyte by a chromatographic detector, wherein the signal curve includes a peak profile of the first analyte. The peak profile is defined by a plurality of measured data points configured to plot onto a signal coordinate system. The method further includes normalizing the peak profile of the first analyte to form a normalized peak profile, wherein the normalized peak profile includes scaling the plurality of measured data points, and wherein the normalized peak profile is defined by a plurality of normalized data points configured to plot onto a normalized coordinate system, and comparing the normalized peak profile of the first analyte with a normalized peak profile of a second analyte. 1. A method of determining an identity of a first analyte in a sample , the method comprising:passing the first analyte through a chromatographic column;detecting a signal curve of the first analyte by a chromatographic detector, wherein the signal curve includes a peak profile of the first analyte, wherein the peak profile is defined by a plurality of measured data points configured to plot onto a signal coordinate system;normalizing the peak profile of the first analyte to form a normalized peak profile, wherein the normalized peak profile includes scaling the plurality of measured data points, wherein the normalized peak profile is defined by a plurality of normalized data points configured to plot onto a normalized coordinate system; andcomparing the normalized peak profile of the first analyte with a normalized peak profile of a second analyte.2. The method of claim 1 , further comprising:identifying the first analyte as the second analyte based upon the comparison.3. The method of claim 1 , wherein comparing the normalized peak profile of the first analyte with the peak profile of the ...

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Номер записи: 69
10-02-2022 дата публикации

Method and System of Identifying and Quantifying A Protein

Номер: US20220043002A9
Автор: Shunhai WANG, Yuetian Yan
Принадлежит: Regeneron Pharmaceuticals Inc

Methods and system for identifying and/or quantifying a protein are provided herein.

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Номер записи: 70
29-01-2015 дата публикации

ANALYTICAL METHODS FOR ANALYZING AND DETERMINING IMPURITIES IN DIANHYDROGALACTITOL

Номер: US20150027206A1
Автор: Lu Xiaoyun
Принадлежит:

An improved analytical method for analysis of dianhydrogalactitol preparations provides a method for determining the purity of dianhydrogalactitol and detecting impurities in preparations of dianhydrogalactitol, as well as identifying any such impurities. The method employs high performance liquid chromatography (HPLC), in particular, HPLC with refractive index (RI) detection; the HPLC can be followed by tandem mass spectroscopy. The method can further comprise the step of performing preparative HPLC collection of at least one specific substance peak present in a preparation of dianhydrogalactitol. 1. An analytical method for analyzing the presence and quantity of impurities present in a preparation of dianhydrogalactitol comprising the steps of:(a) analyzing a preparation of dianhydrogalactitol by subjecting the preparation to high performance liquid chromatography using elution with a mobile phase gradient to separate dianhydrogalactitol from dulcitol and other contaminants of the preparation; and(b) determining the relative concentration of one or more peaks resolved by high performance liquid chromatography that represent compounds other than dianhydrogalactitol itself.2. The method of wherein the compounds other than dianhydrogalactitol itself are at least one of: (1) dulcitol; (2) an impurity other than dulcitol; and (3) a degradation product of dianhydrogalactitol.3. The analytical method of wherein elution is with a gradient of NaOH from about 2.5 mM to about 0.1 mM.4. The analytical method of wherein elution is with a gradient of NaOH from about 1.5 mM to about 0.1 mM.5. The analytical method of wherein elution is with a gradient of NaOH from about 1 mM to about 0.1 mM.6. The analytical method of wherein elution is with a gradient of a combination of ammonium hydroxide and a volatile ammonium salt selected from the group consisting of ammonium formate and ammonium acetate and the total concentration of the ammonium hydroxide and the volatile ammonium salt ...

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Номер записи: 71
29-01-2015 дата публикации

Measuring Multi-Analyte Samples Using an In-Line Flow Cell

Номер: US20150027207A1
Автор: Bartling Donald J., Kramer Kurt D., Le Tong, Likuski Robert K., Prestigiacomo Anthony F.
Принадлежит:

Methods and systems for analyzing ratios of analytes within a flowing sample are provided. The flowing sample can be processed in real-time to determine a time interval over which a predetermined amount of a group of analytes passes by a fixed point in a flow cell. The predetermined amount can be routed to a sample container for future processing. The sample can comprise diluted blood and the analytes can comprise a component of hemoglobin, such as A1c, and the total amount of hemoglobin, of which the predetermined amount is metered. 1. A method for measuring analytes in a sample , the method comprising:flowing a sample containing analytes through a detector;measuring a measurand of the analytes passing through the detector;determining a time interval over which the measurand reaches a predetermined quantity; anddiverting a portion of the flowing sample containing the predetermined quantity of the measurand to a sample volume.2. The method of claim 1 , wherein the measurand has a linear relationship with analyte concentration.3. The method of claim 2 , wherein the measurand is based on light absorbance of analyte.4. The method of claim 1 , wherein measuring comprises performing a real-time integration of the measurand over the time interval.5. The method of claim 4 , wherein the time interval is pre-determined.6. The method of claim 4 , wherein the time interval starts at a first time point that is determined by a first measured threshold of the measurand.7. The method of claim 6 , wherein the sample is flowed into a waste output before the first time point.8. The method of claim 7 , wherein the first time point is the basis to switch at least one valve for diverting the portion of the flowing sample from the waste output to the sample volume.9. The method of claim 4 , wherein the time interval ends at a second time point that is determined by a second measured threshold of the measurand.10. The method of claim 9 , wherein the second threshold is determined by a ...

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Номер записи: 72
24-01-2019 дата публикации

METHOD FOR IDENTIFYING GRAPE SEED EXTRACT AUTHENTICITY USING HPLC FINGERPRINT SPECTRUM

Номер: US20190025265A1
Автор: Gao Wei, LIAN Yunhe, Wang Lei, Yang Qingshan, Zhou Li
Принадлежит: Chenguang Biotech Group Co., Ltd.

Disclosed is a method for identifying grape seed extract authenticity using the HPLC fingerprint spectrum, and in particular, a method for identifying the adulteration of a pine bark extract or a peanut skin extract in a grape seed extract. The method comprises the following steps: 1) establishing the HPLC fingerprint spectrums of the grape seed extract, the pine bark extract and the peanut skin extract, respectively; 2) determining a characteristic peak of pine bark extract and a characteristic peak of peanut skin extract; 3) subjecting the grape seed extract sample to be tested to liquid chromatography detection, identifying the adulteration of the pine bark extract or the peanut skin extract in the sample to be tested according to whether the chromatogram contains the characteristic peaks of the pine bark extract and/or the peanut skin extract, wherein the addition of more than 3% of adulterants can be accurately identified. The method has good stability and reproducibility, high efficiency, obvious identification characteristics, provides a theoretical basis for the identification of the plant sources of grape seed extracts, and is conducive to promoting the healthy development of the plant extract industry. 1. A method for identifying grape seed extract authenticity using HPLC fingerprint spectrum , characterized in that , the method comprises the following steps:S1: establishing HPLC fingerprint spectrums of a grape seed extract, an adulterant pine bark extract and an adulterant peanut skin extract, respectively;S2: comparing the HPLC fingerprint spectrum of the pine bark extract with the HPLC fingerprint spectrum of the grape seed extract to determine the characteristic peak of the pine bark extract; comparing the HPLC fingerprint spectrum of the peanut skin extract with the HPLC fingerprint spectrum of the grape seed extract to determine the characteristic peak of the peanut skin extract;S3: determining a grape seed extract sample to be tested using high ...

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Номер записи: 73
23-01-2020 дата публикации

Analytical Laboratory Testing Procedures

Номер: US20200025721A1
Автор: McChesney Lisa, Newcomb Cecilia
Принадлежит:

A test method developed with the goals of rapid turnaround, short run times (target under 5 minutes), small volumes for dilution (target 10 mL), fewest number of mobile phases, rapid transition between analyses, minimized opportunity for preparation errors, leverage Autoblend® function of equipment, easily maintain on-hand solution inventory, and avoiding ion-pairing agents and other additives. The test method development strategy begins with vendor application notes, USP, and literature references. The strategy targets families of chemically similar compounds, whenever possible, and leverages new column technologies. Finally, the strategy standardizes validation and sample preparation procedures. 1. A liquid chromatography testing method for reducing laboratory waste and testing times , the method comprising the steps of:accepting a plurality of samples to be tested on a liquid chromatograph; limiting a volumetric size of laboratory glassware used to no more than 10.0 mL;', 'limiting transfer of liquids to be no more than 100 microliters;, 'preparing at least one analyte for each of the plurality of samples to create a plurality of test analytes, wherein the step of preparing at least one analyte comprises the steps ofutilizing ultra-high-performance liquid chromatography;selecting autosampler vials to allow analysis of as little as 300 microliters of sample;grouping analytes by families to allow a single chromatographic column to be used for all analytes in a family;running a combination standard for multiple chemicals on the liquid chromatograph; andcreating a subset of the plurality of test analytes based on those containing at least one of the multiple chemicals in the combination standard.2. The method of claim 1 , wherein the families of analytes are selected from the group comprising Antihistamines claim 1 , Antiemetics claim 1 , CII-CIV controlled substances claim 1 , the Caine family claim 1 , the Catacholamine-like family claim 1 , Reversal Agents claim 1 ...

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Номер записи: 74
28-01-2021 дата публикации

Peptide quantitation assay for differentiating full-length high molecular weight kininogen (hmwk) and cleaved hmwk

Номер: US20210025898A1
Автор: Daniel J. Sexton, Gul M. Mustafa, Guodong Zhang, Jiang Wu, Mark SZEWC, Ryan Faucette
Принадлежит: Dyax Corp

Methods for differentiating full-length high molecular weight kininogen (HMWK) and cleaved HMWK in a sample are provided herein. Such methods may comprise treating a biological sample with a protease to generate a plurality of digested peptides, and measuring one or more signature peptides, which are indicative of cleaved HMWK and/or full-length HMWK.

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Номер записи: 75
04-02-2016 дата публикации

METHODS FOR PROCESSING WHOLE BLOOD SAMPLES, AND COMPOSITIONS FOR USE IN PRACTICING THE SAME

Номер: US20160033377A1
Автор: Dillmore Shannon, WANG Ling
Принадлежит:

Methods for processing whole blood samples are provided. Aspects of the methods include depleting leukocytes from a whole blood sample to produce a leukocyte depleted sample, and then lysing red blood cells (RBCs) in the resultant leukocyte depleted sample to produce a leukocyte/RBC depleted sample. Also provided are compositions and kits for practicing embodiments of the invention. The methods and compositions find use in a variety of different applications, including the detection of circulating tumor cells (CTCs). 1. A method for processing a whole blood sample , the method comprising:depleting leukocytes from the whole blood sample via a leukocyte specific binding reagent mediated depletion protocol to provide a leukocyte depleted sample; andlysing red blood cells in the leukocyte depleted sample to provide a red blood cell (RBC) depleted sample.2. The method according to claim 1 , wherein the depleting comprises placing the whole blood sample into a container comprising an enclosed evacuated interior volume and an opening sealed by a septum.3. The method according to claim 1 , wherein the leukocyte specific binding reagent is stably associated with a surface of a solid support.4. The method according to claim 3 , wherein the solid support is an interior surface of the container.5. The method according to claim 3 , wherein the solid support comprises a bead.6. The method according to claim 5 , wherein the bead is a magnetic bead.7. The method according to claim 1 , wherein the leukocyte specific binding reagent comprises an antibody or binding fragment thereof.8. The method according to claim 1 , wherein the leukocyte specific binding reagent comprises an aptamer.9. The method according to claim 1 , wherein the leukocyte specific binding reagent specifically binds to CD45.10. The method according to claim 1 , further comprising collecting any cells present in the RBCs depleted sample.11. The method according to claim 10 , wherein the collecting comprises ...

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Номер записи: 76
04-02-2016 дата публикации

SINGLE CELL CAPTURE WITH POLYMER CAPTURE FILMS

Номер: US20160033378A1
Автор: Dunne Jude, Espinoza Patricio, Griswold Bradley L., Hein Glenn, Husain Syed A., Slater Michael, Srinivasan Maithreyan
Принадлежит:

The present invention provides methods, systems, assemblies, and articles for capturing single cells with a polymer capture film. In certain embodiments, the polymer capture films comprise a plurality of individual channels with top and bottom openings, where the channels are dimensioned such that a single cell is: i) is captured inside the channel, partially or substantially occluding the channel, when negative pressure is provided to the bottom opening; or ii) is captured by the top opening, but does not enter the channel, when negative pressure is provided to the bottom opening. In some embodiments, the channels of the polymer capture film align with the wells of a multi-well chip such that the cell, or the contents of the single cell, may be transferred to a corresponding well. 1. A method of isolating single cells comprising: [ A) has a top opening in said top surface of said polymer capture film,', 'B) has a bottom opening in said bottom surface of said polymer capture film, and', 1) is captured inside said individual channel, partially or substantially occluding said individual channel, when negative pressure is provided to said bottom opening; or', '2) is captured by said top opening, but does not enter said individual channel, when negative pressure is provided to said bottom opening; and, 'C) is dimensioned such that one cell, and only said one cell, from said cell suspension], 'i) a polymer capture film comprising a top surface, a bottom surface, and a plurality of individual channels extending through said polymer capture film, wherein each of said individual channels, 'ii) a pneumatic device that provides said negative pressure, and, 'a) contacting a cell suspension with a cell-isolating system, wherein said cell-isolating system comprisesb) incubating said cell suspension with said cell-isolating system, wherein said pneumatic device provides said negative pressure, such that at least some of said individual channels in said polymer captures film ...

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Номер записи: 77
01-02-2018 дата публикации

CONTROLLABLE INJECTOR SAMPLE DILUTION FOR A LIQUID CHROMATOGRAPHY SYSTEM

Номер: US20180031526A1
Автор: Cormier Sylvain G., Jackson Michael R.
Принадлежит:

Described are a method and a system for injecting a sample into a flow of a liquid chromatography system. The method includes combining a flow of a sample and a flow of a mobile phase to create a diluted sample in the system flow. The volumetric flow rate of the sample is controlled to be at a value that yields a desired dilution ratio for the diluted sample. The particular value at which the volumetric flow rate is maintained can be determined from the desired value of the dilution ratio and the volumetric flow rate of the mobile phase. System embodiments include a syringe that can be used to provide a sample solution at a controllable volumetric flow rate for combination with a high pressure mobile phase. 1. A method for injecting a sample into a flow of a liquid chromatography system , the method comprising:providing a flow of a mobile phase at a first volumetric flow rate; andcombining a flow of a sample at a second volumetric flow rate and the flow of the mobile phase, wherein the combined flows create a diluted sample having a dilution ratio that is responsive to the first and second volumetric flow rates and wherein the first volumetric flow rate is controlled to a value so that the dilution ratio has a predetermined value.2. The method of wherein the sample includes a sample diluent.3. The method of wherein the dilution ratio is responsive to a dilution of the sample by the sample diluent and a dilution of the sample by the mobile phase.4. The method of wherein the sample diluent comprises a solvent that is capable of dissolving a greater quantity of the sample than the mobile phase.5. The method of wherein the mobile phase is a gradient mobile phase.6. The method of wherein the combining of the flow of the sample and the flow of the mobile phase occurs for a predetermined duration.7. The method of further comprising acquiring the sample from a sample source prior to combining the flow of the sample and the flow of the mobile phase.8. A computer program ...

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Номер записи: 78
17-02-2022 дата публикации

System and Method of Matrix Accelerated Vacuum-Assisted Sorbent Extraction for Improved Sample Preparation Prior to GCMS Analysis

Номер: US20220050085A1
Автор: CARDIN Daniel B.
Принадлежит:

Techniques disclosed herein can improve the extraction of chemicals prior to analysis by GC or GCMS. A liquid or solid sample can be placed in a sample container of a closed system under vacuum that further includes a sample extraction device. The assembly can be placed in a 3-zone heater that can separately control the temperature of the bottom of the sample container, the top of the sample container, and the sample extraction device. Vapor flux from the bottom of the sample container into the headspace of the sample container can deliver compounds of interest to the sample extraction device, whereas matrix compounds can re-condense in the headspace of the sample container to avoid delivery to the sample extraction device. Extraction can continue until substantial transfer of compounds of interest to the sorbent occurs, followed by thermal desorption of the extract into a GCMS for analysis. 1. A closed system for preparing a sample under vacuum , the system comprising:a sample container configured to hold the sample;a sorbent;a first heater configured to apply a first temperature to a first portion of the sample container;a second heater configured to apply a second temperature that is less than the first temperature to a second portion of the sample container;one or more heat sinks, fans, or sub-ambient cooling devices configured to maintain the second temperature; anda third heater configured to apply a third temperature that is greater than the second temperature to the sorbent.2. The system of claim 1 , further comprising:a vacuum sleeve configured to form a vacuum-tight seal between the sample container and a sample extraction device containing the sorbent.3. The system of claim 2 , wherein the sample extraction device includes a port or a seal configured to be coupled to a vacuum source while a vacuum is being pulled in the system.4. The system of claim 1 , wherein the sorbent is disposed in a sample extraction device claim 1 , the sample extraction device ...

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Номер записи: 79
31-01-2019 дата публикации

INVERSE GAS CHROMATOGRAPHY STANDARD SOLUTIONS, DEVICE AND METHOD

Номер: US20190033272A1
Автор: MOHAMMAD Mohammad Amin
Принадлежит:

The invention relates to a standard solution for inverse gas chromatography and/or surface energy analysis; a volumetric container for preparing such a standard solution; a method of preparing such a standard solution for inverse gas chromatography and/or a surface energy analysis and a method of probing a solid sample. The standard solution comprises a series of three or more compounds of increasing carbon chain length of general formula (I): R—X wherein: for the three or more compounds R is a series of alkyl, a series of alkenyl or a series of alkynyl groups of increasing carbon chain length; and for all three or more compounds X is H, OH, COH, C(O)H, C(O)CH, NH, SH or halogen; and the relationship between carbon chain length and volume of the compounds of increasing carbon chain length of general formula (I) is determined by the following formula. 2. A standard solution for inverse gas chromatography and/or surface energy analysis according to claim 1 , wherein R is a series of n-alkyl groups of increasing carbon chain length claim 1 , X is H claim 1 , the incubation temperature is 25±1° C. and the relationship between carbon chain length and volume of the compounds of increasing carbon chain length of general formula (I) is determined by the following formula:{'br': None, 'sub': x', 'y, 'sup': 'x-y', 'Relative Volume=3.33Volume±15%'}wherein x is the number of carbons in the carbon chain of the specific compound x;y is the number of carbons in the carbon chain of compound y with the shortest carbon chain length; and{'sub': 'y', 'Volumeis the volume of the compound y.'}3. A standard solution for inverse gas chromatography and/or surface energy analysis according to claim 2 , wherein the standard solution comprises:(i) from 0.85 to 1.15 parts by volume of compound y;(ii) from 2.83 to 3.83 parts by volume of compound y+1;(iii) from 9.43 to 12.75 parts by volume of compound y+2;wherein y is an integer selected from 5 to 38 and is the number of carbons in the carbon ...

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Номер записи: 80
01-05-2014 дата публикации

Collection and Concentration System for Biologic Substance of Interest and Use Thereof

Номер: US20140120537A1
Автор: Chang Ying-Chih, HSU Chia-Hsien
Принадлежит:

A system and method thereof for collecting and concentrating a biologic substance of interest is provided. The biologic of interest obtained from a biologic sample present at an initial low concentration (or low number counts) can be captured and released through a collection device of the system to an intermediate second concentration, and further recovered through a concentration device of the system to a third concentration, thereby facilitating subsequent detection, characterization, enumeration, immunostaining, inspection, imaging, culturing, molecular analysis, and/or other assays. 1. A system for processing a biologic substance of interest from a biologic sample , comprising:a sample inlet configured to receive the biologic sample;a collection device connected to the sample inlet, wherein the collection device comprises one or more surfaces with a surface coating attached to a portion thereon, wherein the surface coating comprises a nonfouling composition and a bioactive composition, and wherein the bioactive composition interacts with the biologic substance of interest present within the biologic sample;a concentration device in fluid communication with the collection device to receive the biologic substance of interest from the collection device and concentrate the biologic substance of interest; anda sample outlet.2. The system of claim 1 , wherein the one or more surfaces of the collection device comprise a plurality of microstructures arranged in one or more patterns thereon and the collection device is a microfluidic device.3. The system of claim 2 , wherein the plurality of microstructures within the collection device is arranged in a pattern selected from the group consisting of one or more lines claim 2 , a horseshoe pattern claim 2 , a herringbone pattern claim 2 , one or more herringbone patterned lines claim 2 , one or more lines of clustered microstructures claim 2 , and various combinations thereof.4. The system of claim 1 , further comprises a ...

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Номер записи: 81
04-02-2021 дата публикации

Method of Diagnosing Oil-Immersed Electrical Apparatus

Номер: US20210033570A1
Автор: Fukutaro Kato, Satoru Toyama
Принадлежит: Mitsubishi Electric Corp

The present invention provides a method of diagnosing an oil-immersed electrical apparatus by an assessment of a state of deterioration of the oil-immersed electrical apparatus including an insulating oil. The insulating oil has been subjected to changing from mineral oil to vegetable oil. The method comprises: performing a first analysis before the changing, by analyzing the insulating oil; performing a second analysis after the changing, by analyzing the insulating oil; performing a first assessment by assessing the state of deterioration of the oil-immersed electrical apparatus before the changing, based on the first analysis results; performing a second assessment by assessing the state of deterioration of the oil-immersed electrical apparatus after the changing, based on the second analysis results; and performing a third assessment by assessing the state of deterioration of the oil-immersed electrical apparatus based on assessment results from the first assessment and assessment results from the second assessment.

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Номер записи: 82
04-02-2021 дата публикации

ANALYZING DATA COLLECTED BY ANALYTICAL INSTRUMENTS

Номер: US20210033576A1
Автор: Day Vivianna, Jarrett Jeremy W., Larkin Michael I., Maurer Barbara R., Trainoff Steven P.
Принадлежит: WYATT TECHNOLOGY CORPORATION

The present disclosure describes a method, a system, and a computer program product of analyzing data collected by analytical instruments. In an embodiment, the method, the system, and the computer program product include receiving set-up information, running at least one incomplete analytical method on at least one known sample on at least one analytical instrument with respect to the set-up information, resulting in known sample data, processing the at least one incomplete analytical method with respect to the known sample data, resulting in at least one validated analytical method, and running the at least one validated analytical method on at least one unknown sample on the at least one analytical instrument with respect to the set-up information, resulting in analyzed sample data. 1. A computer implemented method comprising:receiving, by a computer system, set-up information, wherein the set-up information describes at least one analytical instrument, at least one analysis to be performed on data collected by the at least one analytical instrument, and at least one analytical method, resulting in at least one incomplete analytical method;executing, by the computer system, in response to receiving at least one instruction to automate the at least one analysis, a set of logical operations running the at least one incomplete analytical method on at least one known sample on the at least one analytical instrument with respect to the set-up information, resulting in known sample data;executing, by the computer system, a set of logical operations processing the at least one incomplete analytical method with respect to the known sample data, resulting in at least one validated analytical method; andexecuting, by the computer system, a set of logical operations running the at least one validated analytical method on at least one unknown sample on the at least one analytical instrument with respect to the set-up information, resulting in analyzed sample data.2. The ...

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Номер записи: 83
05-02-2015 дата публикации

HYDROCARBON MODELLING

Номер: US20150039241A1
Автор: Moorwood Roland A. S.
Принадлежит: KBC ADVANCED TECHNOLOGIES PLC

A method of characterizing a mixture having a plurality of hydrocarbons includes: obtaining a set of predetermined pseudo-components, each pseudo-component representing a respective group of hydrocarbons; obtaining physical data about the hydrocarbon mixture; and determining from the data the amount of each pseudo-component considered to be present in the mixture. 1. A method of characterising a mixture comprising a plurality of hydrocarbons , said method comprising:obtaining a set of predetermined pseudo-components, each pseudo-component representing a respective group of hydrocarbons;obtaining physical data about the hydrocarbon mixture; anddetermining from the data the amount of each pseudo-component considered to be present in the mixture.2. A method as claimed in claim 1 , wherein the set of predetermined pseudo-components comprises a distribution of pseudo-components according to the hydrocarbon carbon number.3. A method as claimed in claim 2 , wherein the set of predetermined pseudo-components comprises a distribution of single carbon number cuts.4. A method as claimed in claim 2 , wherein the set of predetermined pseudo-components comprises a distribution of single carbon number cuts grouped together into a number of pseudo-components claim 2 , preferably 4-7 pseudo-components claim 2 , with each pseudo-component comprising more than one single carbon number cut.5. A method as claimed in claim 1 , wherein two or more distributions of predetermined pseudo-components are used.6. A method as claimed in claim 5 , wherein two distributions of predetermined pseudo-components are used and the two distributions of pseudo-components correspond to an aromatic distribution and a paraffinic distribution claim 5 , respectively.7. A method as claimed in claim 1 , wherein the physical data is obtained by at least one of gas chromatography claim 1 , TBP distillation claim 1 , and ASTM D86 distillation.8. A method as claimed in claim 1 , wherein the amount of each pseudo- ...

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Номер записи: 84
07-02-2019 дата публикации

CHEMICAL PRECONCENTRATOR WITH INTEGRATED HEAT SOURCE FOR ENHANCED CHEMICAL ANALYSIS

Номер: US20190041305A1
Автор: Bowers, II Michael J., KING John E., Kippeny Tadd C.
Принадлежит:

A method for detection and identification of a chemical using a preconcentrator to collect a sample of the chemical, process said sample of the chemical, and introduce said processed chemical into a chemical analysis instrument for the detection of and identification of the chemical. A system for detecting and identifying a chemical comprising a preconcentrator having at least dual concentrating elements. 1. A method for detection and identification of a chemical comprising the steps of: collecting a sample of the chemical;', 'processing said sample of the chemical; and', 'introducing said processed chemical into a chemical analysis instrument; and, 'providing a preconcentrator configured for'}detecting and identifying the chemical using said chemical analysis instrument.2. The method of claim 1 , wherein the preconcentrator comprises a solid substrate that can be resistively heated and which has a surface that can absorb and desorb chemicals.3. The method of claim 2 , wherein the substrate and the surface comprise the same material.4. The method of claim 3 , wherein the substrate and the surface comprise silicon carbide foam.5. The method of claim 2 , wherein the substrate and the surface comprise different materials.6. The method of claim 5 , wherein the substrate comprises carbide foam.7. The method of claim 6 , wherein the surface comprises a carbide-derived carbon skin on said solid carbide foam.8. The method of claim 1 , wherein the sample includes a single chemical.9. The method of claim 1 , wherein the sample includes a plurality of chemicals.10. The method of claim 1 , wherein the sample is in a gaseous claim 1 , liquid claim 1 , particulate claim 1 , or aerosol phase.11. The method of claim 2 , wherein the substrate is a solid surface claim 2 , a fiber claim 2 , a powder claim 2 , open cell foam claim 2 , or a mesh.12. The method of claim 1 , wherein both particulate and vapor samples are collected and analyzed simultaneously.13. The method of claim 11 , ...

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Номер записи: 85
07-02-2019 дата публикации

Methods and Systems for Aldehyde Detection

Номер: US20190041332A1
Автор: Brian Young, Bruce Branchaud, Charles Noll, Craig Carlsen, Gerald Thomas, James Ingle, Juven Lara
Принадлежит: Pulse Health LLC

Methods, systems and reagents are provided for detecting and quantifying carbonyl containing moieties in a variety of sample types.

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Номер записи: 86
07-02-2019 дата публикации

PREPROCESSING APPARATUS AND AN ANALYSIS SYSTEM INCLUDING THE PREPROCESSING APPARATUS

Номер: US20190041366A1
Автор: KAWAKAMI Daisuke
Принадлежит: SHIMADZU CORPORATION

To provide a preprocessing apparatus with a function of stirring and sucking a sample in a sample container carried on a conveyor line outside the preprocessing apparatus. 1. A preprocessing apparatus comprising:a sample container setting part in which a sample container that stores a sample is set;a sampling unit which includes a sample probe that sucks a sample in a sample container and is configured to move the sample probe to the sample container set in the sample container setting part, and to an external suction location set on a conveyor line located outside the preprocessing apparatus;a preprocessing container setting part in which a preprocessing container that stores the sample collected from the sample container and dispensed from the sample probe is set;a carrying mechanism which holds and carries the preprocessing container set in the preprocessing container setting part;a preprocessing unit which is disposed at a location to which the preprocessing container can be carried by the carrying mechanism, and performs preprocessing of the sample in the preprocessing container;a unit that stirs a sample before suction which includes a stirring probe that stirs the sample in the sample container, and moves the stirring probe at least to one of the external suction location and a location upstream of the external suction location on the conveyor line; anda stirring operation control unit which is configured to control operation of the sampling unit and the unit that stirs a sample before suction so that a sample in the sample container is stirred by the stirring probe before the sample is sucked by the sample probe.2. The preprocessing apparatus according to claim 1 ,wherein the unit that stirs a sample before suction is configured to move the stirring probe to the external suction location, andwherein the stirring operation control unit is configured to cause the stirring probe to stir at the external suction location a sample in the sample container before ...

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Номер записи: 87
18-02-2016 дата публикации

CRYSTALS OF LAQUINIMOD SODIUM AND IMPROVED PROCESS FOR THE MANUFACTURE THEREOF

Номер: US20160046582A1
Автор: Frenkel Anton, Fristedt Ulf Tomas, Ioffe Vladimir, Jansson Karl-Erik, Laxer Avital
Принадлежит: TEVA PHARMACEUTICAL INDUSTRIES LTD.

The subject invention provides a mixture of Crystalline Laquinimod sodium particles, wherein (i) ≧90% of the total amount by volume of the laquinimod sodium particles have a size of ≦40 μm or (ii) ≧50% of the total amount by volume of the laquinimod sodium particles have a size of ≦15 μm and wherein: a) the mixture has a bulk density of 0.2-0.4 g/mL; b) the mixture has a tapped density of 0.40-0.7 g/mL; c) an amount of heavy metal in the mixture is no more than 20 ppm relative to the amount by weight of laquinimod sodium; d) an amount of MCQ in the mixture is no more than 0.15% relative to the amount of laquinimod sodium as measured by HPLC; e) an amount of MCQCA in the mixture is no more than 0.15% relative to the amount of laquinimod sodium as measured by HPLC; or f) an amount of MCQME in the mixture is no more than 0.12% relative to the amount of laquinimod sodium as measured by HPLC. The subject invention also provides a pharmaceutical composition comprising an amount of laquinimod and at least one of BH-3-HKAQ, MCQ, MCQCA, MCQME, NEA, and MCQEE. The subject invention also provides processes for preparing BH-3-HLAQ, MCQ, MCQCA, MCQEE, and compounds prepared by said processes. Further provided is a process for testing whether a sample of laquinimod contains an undesirable impurity. Further provided is a process for preparing a validated pharmaceutical composition comprising laquinimod, for preparing a pharmaceutical composition comprising laquinimod, or for distributing a validated batch of a pharmaceutical composition comprising laquinimod, for validating a batch of a pharmaceutical product containing laquinimod and a pharmaceutically acceptable carrier for distribution, and for preparing a packaged pharmaceutical composition comprising laquinimod, each comprising determining the amount of at least one of BH-3-HLAO, MCQ, MCQCA, MCQME Подробнее

Номер записи: 88
06-02-2020 дата публикации

METHOD AND APPARATUS FOR INJECTING A CHROMATOGRAPHIC SAMPLE

Номер: US20200041463A1
Автор: Andrews Richard Wayne, Beals Peyton C., Cormier Sylvain Gilles, Fadgen Keith, Gerhardt Geoff C., Gilar Martin, Kornfeld Jordan, McDonald Thomas S.
Принадлежит:

Described are a method and apparatus for diluting a chromatographic sample. The method includes repeating an alternating acquisition of sample fluidic plugs each having an incremental volume of sample and diluent fluidic plugs each having an incremental volume of diluent to obtain a stack of alternating sample and diluent fluidic plugs. The stack is inserted into a flow of a mobile phase in a chromatography system. Alternatively, the method includes repeating the steps of injecting an incremental volume of sample into a chromatographic system flow and providing an incremental volume of mobile phase into the chromatographic system flow. In either implementation, the dilution ratio of the sample equals the sum of the incremental volumes of the sample and the diluent or mobile phase divided by the sum of the incremental volumes of the sample. 1. A method for diluting a sample upon injection into a chromatographic system flow , comprising:a) injecting an incremental volume of a sample into a chromatographic system flow, the sample comprising at least one analyte dissolved in a solvent;b) providing an incremental volume of a mobile phase into the chromatographic system flow; andrepeating steps a) and b) until a total volume of stored sample is injected into the chromatographic system flow, wherein a dilution ratio of the injected sample equals the sum of the incremental volumes of the sample and the mobile phase divided by the sum of the incremental volumes of the sample.2. The method of wherein steps a) and b) are performed by controlling an injection valve in communication with a sample loop and a source of the mobile phase.3. The method of further comprising loading the total volume of the sample into the sample loop prior to a first injection of the incremental volume of the sample into the chromatographic system flow.4. The method of wherein the incremental volume of the sample is proportional to an injection duration and wherein the incremental volume of the mobile ...

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Номер записи: 89
06-02-2020 дата публикации

Hybrid capillary/packed trap and method of use

Номер: US20200041469A1
Автор: Daniel B. CARDIN
Принадлежит: Entech Instruments Inc

A hybrid trap including a replaceable open-tubular capillary trap followed by a packed trap is used to collect, preconcentrate, and recover a sample, such as VOCs and SVOCs found in air. The capillary stage prevents losses and carryover of the heavy fraction and can also collect the particles in air that contain the heavier SVOCs, also preventing them from reaching the packed stage. The packed stage traps lighter organic compounds that are not as prone to carryover due to channeling. The capillary and packed traps together provide quantitative recovery of compounds boiling from as low as −50° C. to as high as 600° C. The sample can be directly desorbed onto the GC column, which avoids losses and contamination caused by other approaches that thermally desorb samples through transfer lines and rotary valves more remote to the GC oven.

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Номер записи: 90
15-02-2018 дата публикации

CHIRAL ANALYSIS METHOD OF BOUND AMINO ACID AND CHIRAL ANALYSIS SYSTEM

Номер: US20180045690A1
Автор: HAMASE Kenji, ISHIGO Shoto, MITA Masashi, MIYOSHI Yurika, UEDA Tadashi
Принадлежит:

A chiral analysis method of a bound amino acid includes a step of hydrolyzing a bound amino acid using a deuterium chloride-deuterium oxide solution and/or deuterium oxide; a step of separating, by chiral separation, a D-form and an L-form of an amino acid generated by the hydrolyzation; a step of generating fragments from the separated amino acid; and a step of selecting and analyzing a predetermined fragment that contains an α carbon and does not contain a side chain from the generated fragments, by mass spectrometry. 1. A chiral analysis method of a bound amino acid comprising:a step of hydrolyzing a bound amino acid using a deuterium chloride-deuterium oxide solution and/or deuterium oxide;a step of separating, by chiral separation, a D-form and an L-form of an amino acid generated by the hydrolyzation;a step of generating fragments from the separated amino acid; anda step of selecting and analyzing a predetermined fragment that contains an α carbon and does not contain a side chain from the generated fragments, by mass spectrometry.2. The chiral analysis method of the bound amino acid according to claim 1 , wherein the D-form and the L-form of the amino acid generated by the hydrolyzation are separated by the chiral separation through a chromatographic method.3. The chiral analysis method of the bound amino acid according to claim 1 , further comprising:a step of derivatizing the amino acid generated by the hydrolyzation,wherein the D-form and the L-form of the derivatized amino acid are separated by the chiral separation.4. The chiral analysis method of the bound amino acid according to claim 1 ,wherein in the step of generating the fragments, the fragments are generated for each of the separated D-form and the L-form of the amino acid, andwherein in the step of analyzing, the predetermined fragment that contains the α carbon and does not contain the side chain is selected for each of the separated D-form and the L-form of the amino acid, and an abundance ...

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Номер записи: 91
06-02-2020 дата публикации

Methods and Systems for Increasing Sensitivity of Direct Sampling Interfaces for Mass Spectrometric Analysis

Номер: US20200043712A1
Автор: Arnold Don W., Covey Thomas R., Liu Chang
Принадлежит:

Methods and systems for delivering a liquid sample to an ion source for the generation of ions and subsequent analysis by mass spectrometry are provided herein. In accordance with various aspects of the present teachings, MS-based systems and methods are provided in which the flow of desorption solvent within a sampling probe fluidly coupled to an ion source can be selectively controlled such that one or more analyte species can be desorbed from a sample substrate inserted within the sampling probe within a decreased volume of desorption solvent for subsequently delivery to the ion source. In various aspects, sensitivity can be increased due to higher desorption efficiency (e.g., due to increased desorption time) and/or decreased dilution of the desorbed analytes. The methods and systems described herein can additionally or alternatively provide for the selective control of the flow rate of the desorption solvent within the sampling interface so as to enable additional processing steps to occur within the sampling probe (e.g., multiple samplings, reactions). 1. A system for analyzing a chemical composition of a specimen , comprising:a reservoir for storing a desorption solvent;a sampling probe having an open end partially defining a sample space configured to receive desorption solvent from the reservoir, said sample space further configured to receive through the open end at a least a portion of a substrate having one or more analyte species adsorbed thereto such that at least a portion of said analyte species are desorbed therefrom into the desorption solvent within the sample space;a plurality of fluid pathways for delivering desorption solvent from the reservoir to an ion source; anda valve movable between a first configuration and a second configuration, wherein in the first configuration a first fluid pathway is provided for flowing desorption solvent from said reservoir to said ion source via said sample space, and wherein in the second configuration a second ...

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Номер записи: 92
08-05-2014 дата публикации

FP PREPARING METHOD, FP PREPARING PROGRAM, FP PREPARING DEVICE, AND FP

Номер: US20140123736A1
Автор: MORI Yoshikazu, NODA Keiichi
Принадлежит: TSUMURA & CO.

An evaluating apparatus includes a FP preparing part that prepares a target FP configured by peaks, retention time points and UV spectra thereof detected from a 3D chromatogram of a multicomponent drug that is an evaluation target at a specific wavelength. 1. A FP preparing method comprising:a FP preparing step preparing a FP configured by peaks and retention time points of the peaks detected from a chromatogram of an evaluation target, whereinthe chromatogram is a 3D chromatogram including retention time points, detection wavelengths, and peaks as data, and the FP preparing step prepares the FP by the peaks, the retention time points and UV spectra thereof detected from the 3D chromatogram at a specific wavelength.2. The method according to claim 1 , whereinthe evaluation target is a multicomponent drug.3. The method according to claim 2 , whereinthe multicomponent drug is one of a crude drug, a combination of crude drugs, an extract thereof, and a kampo medicine.4. The method according to claim 1 , whereinthe FP preparing step prepares the FP that is acquired by composing a plurality of FPs at different detection wavelengths.5. The method according to claim 1 , whereinthe FP preparing step prepares the FP by extracting all the peaks of the 3D chromatogram.6. A computer-readable storage medium storing a FP preparing program for an evaluation target that causes a computer to execute a FP preparing function preparing a FP configured by peaks and retention time points of the peaks detected from a chromatogram of an evaluation target claim 1 , wherein:the chromatogram is a 3D chromatogram including retention time points, detection wavelengths, and peaks as data, and the FP preparing function prepares the FP by the peaks, the retention time points and UV spectra thereof detected from the 3D chromatogram at a specific wavelength.7. The storage medium according to claim 6 , whereinthe evaluation target is a multicomponent drug.8. The storage medium according to claim 7 , ...

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Номер записи: 93
19-02-2015 дата публикации

METHODS FOR FABRICATING HIGH ASPECT RATIO PROBES AND DEFORMING HIGH ASPECT RATIO NANOPILLARS AND MICROPILLARS

Номер: US20150050746A1
Автор: HENRY Michael D., HOMYK Andrew P., Scherer Axel, TOMBRELLO Thomas A., WALAVALKAR Sameer
Принадлежит:

Methods for fabricating of high aspect ratio probes and deforming micropillars and nanopillars are described. Use of polymers in deforming nanopillars and micropillars is also described. 111-. (canceled)12. A method for deforming pillars comprising:providing a plurality of pillars partially submerged in a resist; andcontracting the resist in areas surrounding the plurality of pillars to deform the plurality of pillars.13. The method of claim 12 , wherein the layer of resist is made of Poly methyl methacrylate (PMMA).14. The method of claim 13 , wherein the resist is contracted using an electron-beam having an electron dose claim 13 , a quantity of which is chosen to turn the PMMA into a negative tone resist.15. The method of claim 14 , wherein a force exerted by the PMMA to the plurality of nanopillars is tuned by varying an electron-beam exposure claim 14 , heating claim 14 , or selective resist removal.16. A method for capturing small-scale objects comprising:providing a plurality of small-scale objects surrounded by a plurality of pillars partially submerged in a resist;contracting the resist to bend the plurality of pillars inward to squeeze the plurality of small-scale objects and thereby forcing the plurality of small-scale objects through top of the plurality of pillars.17. The method of claim 16 , wherein the small-scale objects have spherical shapes with diameters of 50 nm or more.1824-. (canceled) The present application claims priority to U.S. Prov. App. No. 61/208,528 filed on Feb. 25, 2009, and U.S. Prov. App. No. 61/164,289 filed on Mar. 27, 2009, both of which are incorporated herein by reference in their entirety. The present application is also related to U.S. Pat. App. No. (Attorney Docket No. P508-US) for ‘Methods for fabricating high aspect ratio micropillars and nanopillars’ filed on even date herewith, the disclosure of which is also incorporated herein by reference in its entirety.The U.S. Government has certain rights in this invention ...

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Номер записи: 94
03-03-2022 дата публикации

SAMPLE SIZE CHROMATOGRAPHY DEVICE

Номер: US20220062790A1
Автор: DASHARATHI Kannan, KRAGNESS Jon P.
Принадлежит:

A chromatography device having a housing formed from an upper housing ultrasonically welded to a lower housing. A compression extension and a boss located inside of the housing with the media disposed between the compression extension and the boss such that a perimeter of the media is compressed between the compression extension and the boss forming a liquid impermeable seal along the perimeter after the upper housing is ultrasonically welded to the lower housing.

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Номер записи: 95
19-02-2015 дата публикации

Systems and Methods to Process Data in Chromatographic Systems

Номер: US20150051843A1
Автор: Wang Jihong, Willis Peter Markel
Принадлежит: LECO Corporation

A system and method for processing data in chromatographic systems is described. In an implementation, the system and method includes processing data generated by a chromatographic system to generate processed data, analyzing the processed data, and preparing and providing results based on the processed data. 181-. (canceled)82. A method of processing data having long clusters and short clusters from a data acquisition system in a chromatography , mass spectrometry system comprising:processing the data to generate processed data;analyzing the processed data to extract noise therefrom;preparing and providing results relating to the processed data,separating the long clusters from the short clusters;filtering the data to smooth the data thereby yielding filtered clusters;dividing the filtered clusters into sub-clusters; andqualifying the sub-clusters to extract undesired sub-clusters therefrom.83. The method of claim 82 , wherein the separating step further comprises:separating the data into blocks;estimating an intensity of a baseline in the center of each block;linearly interpolating between equidistant quartile points of each block to yield a baseline estimation;clipping the data above the baseline level and preserving the data below the baseline; andsmoothing the clipped data to yield an improved version of the baseline.84. The method of claim 83 , wherein estimation of the intensity of a baseline in the center of a block is based on an intensity of the baseline in the lower quartile of the block.85. The method of claim 82 , wherein the qualification step comprises at least one of:selecting sub-clusters that have a signal-to-noise ratio that is greater than a threshold signal-to-noise ratioselecting sub-clusters that have a peak shape that is greater than a threshold quality, andselecting sub-clusters that have a minimum cluster length.86. The method of claim 85 , wherein sub-clusters with a signal-to-noise ratio that is less than the threshold signal-to-noise ...

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Номер записи: 96
25-02-2021 дата публикации

METHOD FOR DETERMINING A DWELL VOLUME OF A CHROMATOGRAPHIC SYSTEM

Номер: US20210055149A1
Автор: DesJardins Christopher, Gilar Martin, Hill Jason F., Tarafder Abhijit
Принадлежит:

Described is a method for determining a dwell volume of a liquid chromatography system and a liquid chromatography system that can determined the system dwell volume. The method includes mixing a flow of a first solvent with a flow of a second solvent to form a solvent mixture. The flows of the first and second solvents are decreased and increased, respectively, to generate a gradient composition. A system pressure of the liquid chromatography system is measured to determine a pressure trace defined as the measured system pressure as a function of time. The dwell volume of the system is determined from a time delay determined between the gradient composition at the mixing location and the pressure trace. The method can be performed with a liquid chromatography system having a chromatographic column or a flow restrictor used in place of the chromatographic column. 1. A method for determining a dwell volume of a liquid chromatography system , comprising:mixing a flow of a first solvent and a flow of a second solvent at a mixing location to generate a solvent mixture in a system flow of a liquid chromatography system;decreasing the flow of the first solvent and increasing the flow of the second solvent over a gradient duration to generate a gradient composition for the solvent mixture;measuring a system pressure of the liquid chromatography system to determine a pressure trace defined as the measured system pressure as a function of time; anddetermining a dwell volume of the liquid chromatography system from a time delay determined between the gradient composition at the mixing location and the pressure trace.2. The method of wherein the solvent mixture has a viscosity and wherein the viscosity changes in response to the gradient composition.3. The method of wherein the change in viscosity is substantially linearly proportional to a change in the solvent mixture according to the gradient composition.4. The method of wherein a viscosity of the first solvent is different ...

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Номер записи: 97
25-02-2021 дата публикации

SOLID PHASE MICROEXTRACTION DEVICE, RESPOSITORY, AND MANIPULATOR

Номер: US20210055192A1
Автор: GOMEZ-RIOS German A., KANE Thomas E.
Принадлежит:

A solid phase microextraction device is disclosed, including a substrate having at least one planar surface, a sorbent layer disposed on at least a portion of the at least one planar surface, a tapering tip extending from the substrate, a receptacle mount configured for removable attachment to an emplacement of a receiving device, and a clocking feature configured for fixing a radial orientation of the planar surface with respect to the receiving device. A solid phase microextraction device repository is disclosed including a wall surrounding a chamber, a plurality of orifices disposed in the wall configured to received and retain the device, and a plurality of clocking feature interfaces disposed in the wall. A solid phase microextraction device manipulator is disclosed, including a manipulator shaft, an emplacement configured to removably engage a receptacle mount, an electrically conductive contact disposed at the emplacement, an ejector, and a clocking feature interface. 1. A solid phase microextraction device , comprising:a substrate having at least one planar surface;a sorbent layer disposed on at least a portion of the at least one planar surface;a tapering tip extending from the substrate;a receptacle mount configured for removable attachment to an emplacement of a receiving device; anda clocking feature configured for fixing a radial orientation of the planar surface with respect to the receiving device.2. The solid phase microextraction device of claim 1 , wherein the clocking feature includes at least one of an indentation or a protrusion corresponding to at least one of a complimentary protrusion or complimentary indentation of the receiving device claim 1 , such that when the solid phase microextraction device is mounted to the receiving device claim 1 , the clocking feature limits the radial orientation of the solid phase microextraction device with respect to the receiving device to a predetermined number of radial positions.3. The solid phase ...

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Номер записи: 98
13-02-2020 дата публикации

PRECONCENTRATION OF FLUID SAMPLES WITH ALTERNATING DUAL LOOP INTRODUCTION

Номер: US20200049602A1
Автор: Saetveit Nathan
Принадлежит:

Systems and methods for automatic preconcentration of fluid samples using alternating dual holding loops are described. A system embodiment includes, but is not limited to, a first sample loop and a second sample loop alternately fluidically coupled with a sample source; a first valve to alternately introduce fluid from the sample source to the first sample loop and the second sample loop; a second valve to alternately receive fluid from the first sample loop and the second sample loop and to alternately provide access to the preconcentration column to fluid received from the first sample loop and the second sample loop; and a pump system configured to alternately introduce sample held in the first sample loop and sample held in the second sample loop to the preconcentration column via the first valve and the second valve. 1. A sample preconcentration system comprising:a first sample loop and a second sample loop alternately fluidically coupled with a sample source;a first valve fluidically coupled with the first sample loop and the second sample loop and configured to alternately introduce fluid from the sample source to the first sample loop and the second sample loop;a second valve fluidically coupled with the first valve to alternately receive fluid from the first sample loop and the second sample loop, the second valve configured to be fluidically coupled with a preconcentration column and to alternately provide access to the preconcentration column to fluid received from the first sample loop and the second sample loop; anda pump system configured to alternately introduce sample held in the first sample loop and sample held in the second sample loop to the preconcentration column via the first valve and the second valve and to alternately introduce sample from the sample source into the first sample loop and the second sample loop.2. The sample preconcentration system of claim 1 , wherein the pump system includes a first pump fluidically coupled with the ...

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Номер записи: 99
13-02-2020 дата публикации

SAMPLE PRETREATMENT METHOD OF MICROEXTRACTION TUBE INJECTION

Номер: US20200049672A1
Автор: Huang Wenyu, Xing Jun, YE Xiaoyu
Принадлежит:

Disclosed is a sample pretreatment method of microextraction tube injection, comprising providing a capillary micro-extraction tube with extracting medium in it as an injector, passing a sample through the capillary micro-extraction tube, during which an analyte is extracted into an extracting medium inside the capillary micro-extraction tube; then, filling the capillary micro-extraction tube with an organic solvent and keeping the filling for a certain period of time, so that the extracted analyte is dissolved in the organic solvent inside the capillary micro-extraction tube to form an injection solution; finally, keeping one end of the capillary micro-extraction tube sealed and inserting the other end directly into an injection port of a gas chromatography, such that the injection solution is automatically ejected out from the capillary micro-extraction tube into the injection port. 1. A sample pretreatment method of microextraction tube injection , comprising:providing a capillary micro-extraction tube with extracting medium in it as an injector,passing a sample through the capillary micro-extraction tube, during which an analyte is extracted into the extracting medium inside the capillary micro-extraction tube;then, filling the capillary micro-extraction tube with an organic solvent and keeping the filling for a certain period of time, so that the extracted analyte is dissolved in the organic solvent inside the capillary micro-extraction tube to form an injection solution; andfinally, keeping one end of the capillary micro-extraction tube sealed and inserting the open end directly into an injection port of a gas chromatography, such that the injection solution is automatically ejected out from the capillary micro-extraction tube into the injection port to complete the injection operation for gas chromatography;wherein, the extracting medium inside the capillary micro-extraction tube does not exceed 90% of the total internal volume of the capillary micro- ...

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Номер записи: 100