Method for refolding recombinant endostatin
Номер патента: WO2001060839A9
Опубликовано: 17-10-2002
Автор(ы): Dong Xie, John Erickson, Paul Grulich
Принадлежит: Dong Xie, John Erickson, Paul Grulich, Us Health
Опубликовано: 17-10-2002
Автор(ы): Dong Xie, John Erickson, Paul Grulich
Принадлежит: Dong Xie, John Erickson, Paul Grulich, Us Health
Реферат: A simple and reproducible method for refolding endostatin is provided. Recombinant endostatin isolated from bacterial inclusion bodies of large scale cultures can be refolded using this method. Refolding is accomplished by contacting endostatin with cyclodextrin in an aqueous medium buffered at a neutral or acidic pH (preferably, a pH of about 4 to about 5). The refolded endostatin has similar biological activity, including anti-proliferation activity against stimulated endothelial cells in culture, as native endostatin.
Method for refolding recombinant endostatin
Номер патента: WO2001060839A2. Автор: John Erickson,Dong Xie,Paul Grulich. Владелец: The United States Of America, As Represented By Secretary Of The Department Of Health And Human Services. Дата публикации: 2001-08-23.