07-04-2023 дата публикации
Номер: CN115927198A
Принадлежит:
The invention relates to a drug screening model for inhibiting NAAA activity and a construction method thereof, the drug screening model is an HEK293T cell transfected by NAAA lentivirus with a reporter gene tag, a reporter base is an anti-biological resistance gene, a plasmid carrying the reporter gene is pLV-EF1-puro cs2.0-C-Flag, the position of an insertion fragment is 3345bp-4451bp, the insertion fragment comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 6, and SEQ ID NO: 6. The invention also relates to a construction method of the drug screening model for inhibiting the NAAA activity, which comprises the following steps: constructing recombinant plasmids, culturing HEK293T cells, transfecting the HEK293T cells, and adding the transfected HEK293T cells into an NAAA substrate-buffer solution to establish the NAAA inhibitor screening model.
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