Tissue compositions using cultured fibroblasts and keratinocytes and methods of use thereof

Compositions comprising a biodegradable material having a positive charge, fibroblasts, and keratinocytes, methods of forming the compositions, and methods for treating tissue conditions or disorders using the compositions are provided. The preferred biodegradable material having a positive charge is crosslinked polysaccharide having a positive charge. The compositions are particularly useful in treating various skin tissue conditions or disorders.