Traditional Chinese medicine oral liquid for treating livestock and poultry respiratory disease

Technical Field

The invention belongs to the field of veterinary medicine, in particular to a used for the treatment of livestock and poultry traditional Chinese medicine oral liquid of respiratory tract diseases.

Background Art

In recent years, along with the expansion of the scale of intensive cultivation, livestock and poultry diseases of the respiratory system, the digestive system diseases more serious diseases. At the same time as the antibiotic drugs of abuse of the cultivation for in livestock and poultry, the problem of drug-resistant strain become more and more serious.

In poultry diseases of the respiratory system, for example, the outbreak of diseases of the respiratory system, seriously affected the [...] and the utilization rate of feed, make laying rate is reduced, the quality of the carcass downstaged, or even cause a considerable number of death, affected the economic benefits of raises the birds and beasts. Poultry raises the birds and beasts the disease of the respiratory tract is more complicated in production, there are many kinds of diseases, caused by a virus, caused by a bacteria and mycorplasma, morbidity and mortality ranged.

Currently, the pharmaceutical market as an antibiotic drug, used for the treatment and prevention of respiratory tract diseases, these drugs although the dosage is small, fast effect, however, are often unable to treatment of its fundamental, also easy to produce the drug-resistant strain. The use of traditional Chinese medicine for treating livestock and poultry respiratory disease has become an effective means of one of the treatment, however the current clinical use of traditional Chinese medicine preparation for powder, the presence of the defect, and because of the short alimentary tract of poultry, can not Digest lignocellulolytic, the drug absorption difference caused by, large consumption and the like.

Content of the invention

Based on the above-mentioned situation, the purpose of this invention is to provide a kind of used for the treatment of livestock and poultry traditional Chinese medicine oral liquid of respiratory tract diseases.

The purpose of this invention is realized like this: with clearing away heat and toxic materials, the effect of relieving cough traditional Chinese medicine oral liquid phlegm, characterized in that porcine gall powder 1-5 parts, stemona root 13-45 parts, aster tataricus L. F. 26-90 parts.

Preferably: porcine gall powder 3 parts, stemona root 39 parts, aster tataricus L. F. 78 parts.

A used for the treatment of livestock and poultry compound Chinese medicinal preparation of respiratory tract diseases, characterized in that said compound of a traditional Chinese medicine preparation for oral liquid dosage form.

Veterinary dialectical in in accordance with the, can be regarded as epidemic invading into the country respiratory tract diseases, lung and stomach exuberance heat, stagnation of blood. By clearing away the mild, antibacterial, anti-infectious-based.

Pulvis Fellis suis: nature pain , cold, a clearing away heat and toxic materials, dryness, and the effects of asthma;

Stemonine: sweet flavor, myoporum, tepefaction, elelemtns and is, pesticidal efficacy;

Aster tataricus L. F.: nature Xin , myoporum temperature, the lower a moistening, the efficacy of the phlegm cough.

Three drug combination, with clearing away heat and toxic materials, elelemtns and, the effects of asthma.

General the industry of the present invention prepared by the method of: stemona root, aster tataricus L. F. the two medicinal materials groundsel, 1st time 8 times of water, at 2nd 6 times of water, each time the after boiling, 2 hours, to take advantage of heat 200 mesh filter screen. Combining the two filtrates, adding the pig gallbladder powder mixes uniform , to be equivalent to each milliliter of concentrated to 1g, refrigerated 24 hours. Pulp board after filtering the loss of fill, then refrigerated 24 hours, after the cold storage in order to 3 the the amount of [...] the hydroxy benzoic acid methyl ester, mixing, circulation of steam sterilization 30 minutes, quality testing, filling, to obtain.

In a specific experiment under the effect of the present invention:

1   material and method

1.1   test material

1.1.1   test pharmaceutical medicament of this invention, the main component is porcine gall powder, stemona root, aster tataricus L. F., recommended dose 0.75 ml/only; to control medicament hemp apricot Shi Gantang , in the original dosage raw material for medicine idea , veterinary pharmacopeia hemp apricot Shi Gansan amount of light.

1.1.2 experimental animal   10 day old SPF chick embryo, provided by Nanjing medicine applicator factory ; 1 date age Ivy cushion health poulard 550 only, from a certain Nanjing hatching factory.

1.1.3   strain and strain chicken infectious bronchitis respiratory tract toxic (IBV-M₄₁), for poisonous price 105.15/0 . 1 ml.

Rejuvenation and with measuring poisonous price : toxic will be lyophilized 1:10 dilution, the 10 day old SPF chorioallantoic cavity, 0.1 ml/embryo, for the 37 culture [...] 30-36h rear, aseptic collecting the allantoic fluid. After inspection, venom aseptic, for poisonous price 106/0 . 1 ml, for for attacks the poison.

1.2   test method

1.2.1   manual-induced chicken respiratory tract infectious bronchitis pre-test 15 day old for test chicken 180 only, weight 135.4 ± 1.3g, random are divided into 5 a and contamination group 1 a negative control group, each group of 30 only. Separate compartment cage, conventional breeding management. Each contamination group into chicken nasus the venom of a different number of chemicals. The negative control group of chicken not contamination. The chicken tracking observation and inspection disease chicken, in accordance with its toxicantexposure morbidity and mortality time, quantity, whether the disease characteristic symptoms appear and, computing the ratio cfrs, determined for artificial induced drop nose quantitytries the venom of this disease.

1.2.2   for treating artificial inducing chicks respiratory tract infectious bronchitis test 25 day old for test chicken 350 only, weight 181.5 ± 1.5g, as indicated in the schedule packet, contamination, to medicine, tracking and observing the 3 week. Compared with the clinical changes in each group of chickens, pathological vesting, according to its cure rate, recovery rate, mortality, the total efficiency and the like, the medicine of the present invention a comprehensive assessment of the efficacy of this disease. Packet shown in table 1.

2   results

2.1   manual-induced chicken respiratory tract bronchitis test results

According to the test results, the test only for the chick the nose drips into 0.2 ml for tries the venom , successful produce respiratory tract infectious bronchitis.

Table 1   test chicken packet

2.1.1 toxicantexposure group observation   of clinical symptoms in the chicken toxicantexposure 20-54h all disease, its major symptoms are uneasy or somnolence, or reduced rationswaste food , hi drinking, squats lies , riding bikes, shook, rhinorrhoea, sneeze, cough with dyspnea, tracheal [...] , row of dilute white soft it. IB the fine jade expands test showed positive reaction. The toxicant 3.5d has begun to dead chicken, continue to observe the end of one week, see dead chicken, the total rate of 90%, more than the exposure amount to the death of all groups of chickens, the exposure amount is lower than the present group of chickens number of different levels and different onset of death. Chicken all lives healthily negative control group.

2.1.2   disease chicken inspection disease chicken meatus, nasal, tracheal, bronchial mucosa turgesce, containing in the cavity a different number of slurry, mucus or cheese-like exudate; bronchium surrounding spread small inflammation range; airbag opacities; kidney turgesce pale, the renal tubule and ureter grooves cyanurate; catarrhal enteritis, Bursa of Fabricius and other zoki in abnormal.

The results of the whole, with clinical infectious bronchitis respiratory tract and pathological change characteristic laws of death and morbidity. This test confirmed that the chicken is IBV-M₄₁ with death the onset, the liquid according to the above-mentioned for the phenomenon of the chicken dropping nose toxicantexposure induce the disease is feasible. Manual-induced the disease chicken can be used for testing for drug therapy.

2.2   treatment result shown in table 2

2.2.1   clinical observation

Positive control in contamination of the chickens 1 days respiratory tract infectious bronchitis have symptoms, IB the fine jade expands test showed positive reaction, test period survival 10 only, death 20 only, mortality rate is 66.7%. Chicken all lives healthily negative control group. Medicine and contrast reagent for each group after toxicantexposure chicken at the same time also the characteristic symptoms of disease. The administration of the each group of the chicken tracking observation, high of the drug of the invention, in the medicine dose group chicken 2-3d condition obviously improved, the cure rate and the total efficiency as indicated in the schedule, are obviously higher than low dose of the drug of the invention and compared with the pharmaceutical.

2.2.2 the bacteriological inspection result   splits examinessplits examines each group only by ingestion of the chicken, and the air taking pipe mucosal scraping, lung tissue, blood, for bacteriological examination, results consistent with chicken infectious bronchitis respiratory tract the pathological change characteristic.

Table 2   the medicine of the present invention testing respiratory infectious bronchitis the curative effect of the

2.2.3   regression test results will be each group of typical tracheal mucosa dead chickens malade scraping and lung organ, are respectively in 16 only 26 for health ages in the test of the chicken. 27-28h in characteristic after the symptoms of this disease, 8d to the death of all the; see splits examines the disease characteristic lesion. Confirmed as infectious bronchitis virus causing death.

2.2.4   the test is finished, the test results according to the survival of chicken the group survival chicken whether the symptoms of this disease, pathological changes and its extent, of photophygous the fungus examineschicken number , in the schedule for calculating the cure rate of, for example, recovery rate, the total efficiency. Recognizing that high dose of the medicine of the present invention better curative effect.

3   discussion

3.1   the medicine of the present invention the infectious bronchitis

The medicine of the invention is compound preparation of Chinese medicine, the test confirmed that chicks scale the medicine for treating respiratory tract infectious bronchitis pharmacodynamic indeed,. According to test efficacy, dose will be recommended as clinical use 0.75 ml/only, mixes drinks administration.

3.2   the invention the safety of the drug

The safety test the health of the chicken (10 times recommended dose drinking water, together 5 days) shows that, during use of the performance not see clinical abnormalities, chicken has no obvious effect on the health, weight weight difference does not notably and blank control group. The safety of the preparation is.

The beneficial effect of the invention: it can be seen through the therapeutic effect experiment, for the treatment of livestock and poultry respiratory diseases indeed, suitable for popularization and application in the animal production. At the same time the dosage form of this invention effectively combines the short reproductive tracts of birds, can not Digest the wood fiber of the special physiological structure, the medicament into oral liquid, the dispersed rapidly in drinking water, after the drinking water is absorbed trouble birds and beasts , can achieve the purpose of fast onset.

Mode of execution

Embodiment 1: porcine gall powder 1 parts, stemona root 13 parts, aster tataricus L. F. 26 parts, stemona root, aster tataricus L. F. the two medicinal materials groundsel, 1st time 8 times of water, at 2nd 6 times of water, each time the after boiling, 2 hours, to take advantage of heat 200 mesh filter screen. Combining the two filtrates, adding the pig gallbladder powder mixes uniform , to be equivalent to each milliliter of concentrated to 1g, refrigerated 24 hours. Pulp board after filtering the loss of fill, then refrigerated 24 hours, after the cold storage in order to 3 the the amount of [...] the hydroxy benzoic acid methyl ester, mixing, circulation of steam sterilization 30 minutes, quality testing, filling, to obtain.

Clearing away heat and toxic materials, antitussive expectorant. Suitable for respiratory tract infection of livestock and poultry, such as infectious bronchitis and other diseases of the respiratory system. Poultry: 0.75 ml/only, two times a day, free drinking, together 3-5 days; the severe double quantity ; livestock: Pig, cattle, 0.45 ml/kg body weight, twice a day, free drinking, together 3-5 days.

Embodiment 2: porcine gall powder 3 parts, stemona root 39 parts, aster tataricus L. F. 78 parts, stemona root, aster tataricus L. F. the two medicinal materials groundsel, 1st time 8 times of water, at 2nd 6 times of water, each time the after boiling, 2 hours, to take advantage of heat 200 mesh filter screen. Combining the two filtrates, adding the pig gallbladder powder mixes uniform , to be equivalent to each milliliter of concentrated to 1g, refrigerated 24 hours. Pulp board after filtering the loss of fill, then refrigerated 24 hours, after the cold storage in order to 3 the the amount of [...] the hydroxy benzoic acid methyl ester, mixing, circulation of steam sterilization 30 minutes, quality testing, filling, to obtain.

Clearing away heat and toxic materials, antitussive expectorant. Suitable for respiratory tract infection of livestock and poultry, such as infectious bronchitis and other diseases of the respiratory system. Poultry: 0.75 ml/only, two times a day, free drinking, together 3-5 days; the severe double quantity ; livestock: Pig, cattle, 0.45 ml/kg body weight, twice a day, free drinking, together 3-5 days.

Embodiment 3: porcine gall powder 5 parts, stemona root 45 parts, aster tataricus L. F. 90 parts, stemona root, aster tataricus L. F. the two medicinal materials groundsel, 1st time 8 times of water, at 2nd 6 times of water, each time the after boiling, 2 hours, to take advantage of heat 200 mesh filter screen. Combining the two filtrates, adding the pig gallbladder powder mixes uniform , to be equivalent to each milliliter of concentrated to 1g, refrigerated 24 hours. Pulp board after filtering the loss of fill, then refrigerated 24 hours, after the cold storage in order to 3 the the amount of [...] the hydroxy benzoic acid methyl ester, mixing, circulation of steam sterilization 30 minutes, quality testing, filling, to obtain.

Clearing away heat and toxic materials, antitussive expectorant. Suitable for respiratory tract infection of livestock and poultry, such as infectious bronchitis and other diseases of the respiratory system. Poultry: 0.75 ml/only, two times a day, free drinking, together 3-5 days; the severe double quantity ; livestock: Pig, cattle, 0.45 ml/kg body weight, twice a day, free drinking, together 3-5 days.