NOVEL MELON PLANTS

The present invention refers to the new taste, which are capable of producing fruit provides plant melon. Specifically, the present invention refers to organic acid content, pH combination of new and sugar content, which are capable of producing fruit provides plant melon. An organic acid concentration of the present invention inventor are various melon fruit has been confirmed. Furthermore, content of the present invention inventor relative malic and citric acid are various melon fruit has been confirmed. Furthermore, the lower-pH traits of the present invention inventor background melon suite of fruit by introducing pH reduction and citric acid content are obtained it is confirmed that the n bit parallel data inputted, further melon fruit taste of properties which can be operated by a until identifying improving on the. According to one aspect, of the present invention and comprising at a small amount of fruit melon-malic acid. Therefore, the present invention refers to the present specification as described citric acid pH is content for producing the melon fruit has been deteriorated disclosure the plant. According to one aspect, the present invention according to a sugar concentration that observed in fruit of plants in rolled, both rollers are rolled present melon suite circulating at a concentration to maintain or is of an increased concentration. Therefore, and non-greasy to new taste and the plant of the present invention capable of producing of fruits.. The present specification described pH, citric acid and malic content, and sugar content measurements of the as such as in the embodiment 1 to 5 the embodiment. Table 1A 1B table and the present invention according to a representative melon plants and their fruit is formed inside the tie. disclosure.

In one aspect, and the plant melon 100g live weight citric acid content of the present invention approximately 400 milligram (fwt) capable of producing of fruits at least.. In one aspect, of the present invention about 4.2 to about 5.6 pH plants are melon fruit in. capable of producing. In one aspect, of the present invention at least about sugar content plants are melon 5.0g/100g fwt. capable of producing of fruits. In one aspect, the present invention refers to citric acid content of about 400 milligram/100g fwt at least, is between about pH 4.2 to about 5.6, sugar content of fruits at least about 5.0g/100g fwt, which are capable of producing melon disclosure the plant.

In one aspect, the present invention refers to full ripeness to

A) 100g fwt citric acid between approximately 400 milligram to about 1,200 milligram;

B) pH between about 4.2 to about 5.6; and

Between approximately c) 100g fwt 15.0g to about 5.0g a fruit containing fermentable sugars, which are capable of producing melon disclosure the plant.

In one aspect, fruit citric acid content of about 400 milligram to about 1,000 milligram/100g fwt is. In one aspect, fruit citric acid content of about 450 milligram to about 950 milligram/100g fwt and, in one aspect is about 475 milligram to about 900 milligram/100g fwt. In one aspect, pH of about 4.3 to about 5.4 fruit between and, in one aspect about 4.4 to about 5.1 between. In one aspect, about sugar content of fruit between 13.0g/100g fwt to about 5.5g. About sugar content of fruit in one aspect between 15.0g/100g fwt to about 7.0.

In one aspect, said fruit of plants present in the ratio of citric acid to malic is greater than 4.4, in one aspect being greater than 5, greater than 10 in one aspect. In one aspect, said fruit of plants malic to citric acid present in the ratio of less than 450, less than 200 in one aspect, less than 150 to one aspect. In one aspect, about content malic of said fruit of plants less than 85 milligram/100g fwt, 75 milligram/100g fwt less than about in one aspect.

In one aspect said 6 to sucrose present in the fruit of plants the party decision which burns ratio of about 1:1, in one aspect about 1:1 to about 1:2, in one aspect is about 1:1 to about 2:1.

In one aspect, said color is orange fruit body during fruit of plants, white, green or yellow is. In one aspect, in lifestyle adding water fruit. In one aspect, during fruit flowers, roots and Barks of said live fruits weight 50% of a total middle rind. much larger than present. In one aspect, melon plant and comprising at a low pH traits. In one aspect, the transgenic plant family of IND-35 low pH (representative seed is NCIMB 41202 is filed under access), or said IND-35 can be obtained from progeny series. In one aspect, the transgenic said low pH-homo-junction in plants is-hetero-junction or.

According to one aspect of the present invention, "citric acid +" plant that flowers, roots and Barks of melon is disclosure. Such plant the new sensate the scan electrode driver applies a very a sour fruit juice stage capable of producing.. Such fruit citric acid pH is, and low in a large content, . is felt on the sour increased and buys the taste. Is taste and are canceled against high sugar content, according to one aspect reducing sugar (oligosaccharide exposed) with a rise of concentration is the LCD panel displays the Image. Reducing sugar high content. thus it is eminently initial adolescence in particular. Rolled, both rollers are rolled in melon suite being distributed cannot realistic sensation the combination of a sour and will know if consumer. Such scene the combination of melon suite scene sensate the field of. is provided to satisfy with a desire. The sour of the fruit in addition an early [...]. the masks its taste of fruit maturation. According to one aspect, such as system IND-35 citric acid plant is generally derived from a low pH (oriental melon) melon traits obtained a then introduced to a background. In one aspect, one or more of the following finger signal melon criteria accordance with features of the images the: high citric acid content, low malic content, high citric acid to malic content, high sugar content, high content service zone and deactivates other 6, high non-sucrose to service zone and deactivates other 6, high-juice. Desirable upon incorporation of low pH is obtained offspring until said. selecting carefully the features.

For example, in the embodiment 9, 10 and 11 for such plant is described in which in work sacrifice. Furthermore, in accordance with features desired plants may also melon replacement the selecting, a can be as described specification is used as starting materials.

In one aspect, the fruit of plants full ripeness to citric acid of the present invention

Citric acid per a) 100g fwt between about 600 to about 1,200 milligram;

B) pH between about 4.2 to about 5.1;

Between approximately c) 100g fwt 15.0g to about 5.0g. containing fermentable sugars.

In one aspect, fruit citric acid content of about 600 milligram to about 1,000 milligram/100g fwt is. In one aspect, fruit citric acid content of about 650 milligram to about 950 milligram/100g fwt is.

In one aspect, pH of fruit between about 4.4 to about 5.0.

In one aspect, about sugar content of fruit between 13.0g/100g fwt to about 7.0g. In one aspect, said fruit of plants present in the ratio of citric acid to malic is greater than 6, in one aspect 7 being greater than, greater than 10 in one aspect. In one aspect, said fruit of plants malic to citric acid present in the ratio of less than 450, less than 200 in one aspect. In one aspect, about content malic of said fruit of plants less than 85 milligram/100g fwt, 75 milligram/100g fwt less than about in one aspect, less than 60 milligram/100g fwt about in one aspect.

In one aspect, the present invention according to the fruit of plants at eating color green or white and full ripeness to

Citric acid per a) 100g fwt between about 600 to about 1,200 milligram;

B) pH between about 4.2 to about 5.1;

Between approximately c) 100g fwt 15.0g to about 5.0g. containing fermentable sugars.

In one aspect, fruit citric acid content of about 600 milligram to about 1,000 milligram/100g fwt is. In one aspect, said fruit at eating is green or the about-sugar to full ripeness is 12.0g/100g fwt to about 8.0g. In one aspect, at eating is green or the fruit full ripeness ratio of about malic to citric acid to between 200 to 25. In one aspect, said fruit of less than 50 milligram/100g fwt about content malic, 30 milligram/100g fwt less than about in one aspect. In one aspect, said 6 to sucrose present in the fruit of plants the party decision which burns ratio of about 1:1, in one aspect about 1:1 to about 2:1 between.

In one aspect, the present invention according to color is orange fruit of plants has matrix shape full ripeness to

Citric acid per a) 100g fwt between about 600 to about 750 milligram;

B) pH between about 4.5 to about 5.1;

Between approximately c) 100g fwt 13.0g to about 6.0g. containing fermentable sugars.

In one aspect, said fruit malic to citric acid present in the ratio of between about 4.4 to 30. In one aspect, about sugar content of fruit between 13.0g/100g fwt to about 7.0.

In one aspect, the present invention refers to suitable fruit-(mild-fruity) sour is a flavored, which are capable of producing of fruits sweet the existing ways to melon, "citric acid-" melon plant or fruit is bonded disclosure the plant. Such fruit citric acid content of relatively high the pH. adequately low. Such complete after has pH adequately low RC, showing live fruit of mature fruit flavor and color complete without impacting the expression, still taste masking the taste of fruit WIPO free fruit of sour is felt on the.. Furthermore, mature during (mature early to late), fruit increased suitable pH, low combined with a reduction of acidity by citric acid content exhibits a brittle buffer ability. The, very sweet and all parts of a good and flavor and is transparent melon a very slightly water sour to be expressed complete it is intended to provide a fruit shrinp. Melon flavor usually step mature fruit power device is significantly expressed complete sweetened, mainly and direction is right and left. And the expected values and direction sugar-melon WIPO hereinafter and other flavor components, in unpleasant it is not there is tasteless super-: a buffer is melon. Strawberry, orange or peach the of fruits other data such as plus most pronouncedly buys the taste WIPO step. Suitable fruit-flavored high stage the combination melon overcome risk of tasteless super of melon WIPO. or limiting.

In one aspect, such as system IND-35 citric acid plant is generally derived from a low pH traits obtained a [...] melon then introduced to a background. In one aspect, the following background melon [...] ; at least one criteria accordance with features of the images the: low citric acid content, low malic content, relatively high citric acid to malic content, high sugar content, high sucrose content. Desirable upon incorporation of low pH is obtained offspring until said. selecting carefully the features.

For example, such in the embodiment 11 is described in which for in work sacrifice of plants. Furthermore, in accordance with features desired plants may also melon replacement the selecting, a can be as described specification is used as starting materials.

In one aspect, the fruit of plants full ripeness to citric acid of the present invention

Citric acid per a) 100g fwt between about 400 to about 650 milligram;

B) pH between about 4.6 to about 5.6;

Between approximately c) 100g fwt 13.0g to about 5.0g. containing fermentable sugars.

In one aspect, fruit citric acid content of about 450 milligram to about 600 milligram/100g fwt is. In one aspect, pH of fruit between about 4.8 to about 5.4, in one aspect to about 5.2. In one aspect, about sugar content of fruit between 13.0g/100g fwt to about 5.0g, in one aspect, about between 12.0g/100g fwt to about 6.0g. In one aspect, about sugar content of fruit between 13.0g/100g fwt to about 7.0g.

In one aspect, the present invention according to the fruit of plants full ripeness to and orange color at eating

Citric acid per a) 100g fwt between about 400 milligram to about 550 milligram;

B) pH between about 4.8 to about 5.6;

Between approximately c) 100g fwt 11.0g to about 5.0g. containing fermentable sugars.

In one aspect, said fruit malic to citric acid present in the ratio of between about 4.4 to 10. In one aspect, about sugar content of fruit between 11.0g/100g fwt to about 7.0g.

The present invention refers to operation degree of sour of fruit-flavored having useful connection structure is a structural to induce variations. Sour an organic acid content together and compositions, the most important variable is one of an pH as a variable is a result of an interaction between. PH has taste and which are involved in the acid in the dissolution of a great impact on.. Each acids are pH which differ from each other in a dissocation constants (pKa), at this time of H ion and accomplished by relating is dissociated into 50%. Low pH the dissociated form of acid-rich.. Sour perceptual mainly on the basis of the shape american nautical mile of.. High pH level in weak acid such as organic acids large pKa is strong which is why feel in than..

Concentration of acid has pH of solution and is connected to the semiconductor layer. relation and pKa. [...] strong acid solution, has a much lower pH than weak since the let. Concentration of acid (acidity can be proper) has a high degree of high if scene resulted in smaller dimensions will that the user feels the acid. Sour the chemical structure of sour in addition, number of carboxyl, molecular weight and correlate with polarity of molecules (e.g., PURAC Biochem, Gorinchen, The Netherlands, "Flavor Special", www.Purac.com). Sour in addition each food acid a duration, the reception of a, other than sour strength and flavor taste, e.g.-convergent, bitter and sweetened, based on contribution to 0 or 1. flavor a unique identifier broadcast.

Melon fruit main organic acid has a predetermined succinic acid, malic is and green (Wang et al. (1996) J. Agric. Food Chem. 44 : 210-216). Malic is very function, and waits for a step or fruit eudragit e, eudragit AEA, that is associated with a step modified and laver prone and subject, generally favored malic in taste preferred do not go. Near steel seed at eating fruit hexagon which is similar in a region proximate to the peel fruit from malic. is observed with rising content. The malic. low pKa than citric acid. Citric acid more high clean taste and in is, taste or sometimes other co-catalysts overwhelm the characterized of perfume. Unlike malic, seed steel from mature further near the fruit peel outside at eating fruit as hexagon which is similar in. is observed with slope content reduction citric acid. Such a per. observed even.

I.e., at a high data rate in a fruit melon the present invention according to citric acid and high sugar content of fruit content are pleasant tasting areas having the high content of a two component said very and non-greasy to the new fruit matrix shape providing taste and flavor and is thus tended to associated.

Furthermore, fruit melon of the present invention the perceptual sour in on screen character generating citric acid is pH when 2000.

In one aspect, the present invention according to taste trained fruit consisting taster reasons the visitors were by panel the Internet. 0 fruit functional features in has been determined according to judging criterion up to 9. Fruit of pH, organic acid content has been determined also and sugar content. Expert panel should be noted that the results of in the embodiment 12, table 14 and a've got the to 15. The present invention according to such functional analysis fruit acid present in the fruit taste is a function of pH by citric acid content and connection structure is a structural. Maturation and function, and waits for based on result collected in fruit, fruit it buys with taste expert panel of selected content and pH, thereby was enters a command to equation: acid taste = 13, 12-(2.97 x pH) + (0.00587 x citric acid content (milligram/100g fwt)), r2=0.70.

Therefore, in one aspect, the present invention refers to expert panel taste acid which are measured by means of a least about 0.5, which are capable of producing of fruits disclosure a plant melon. In one aspect, the present invention refers to acid taste at least about 1.6, which are capable of producing of fruits disclosure a plant melon. In one aspect, the present invention refers to acid taste taste sugar at least about 0.5 at least about 4.3, which are capable of producing of fruits disclosure a plant melon. In one aspect, the present invention refers to acid taste is between about 1.6 to about 3.8 taste sugar is between about 4.3 to about 5.8, which are capable of producing of fruits disclosure a plant melon.

In one aspect, the present invention refers to acid taste is between about 2.5 to about 3.8 taste sugar is between about 4.3 to about 5.6, which are capable of producing of fruits disclosure a plant melon. Such plant of a typical example of hybrid-based YUSOL X SOLAZ and is Mehari X L53 AZ B.

In one aspect, pH and green content such fruit the present specification is described. In one aspect, the present invention refers to acid taste is between about 1.6 to about 3.0 5.8 to about 5.2 about taste sugar is between of fruits, which are capable of producing melon disclosure the plant. Such plant representative examples hybrid based YUSAZ X YUSOL, . TD X L53 AZ A and Mehari X L53 AZ A.

In one aspect, pH and green content said fruit the present specification is described. In one aspect, the present fruit such as described to specification pH, organic acid content and compositions which has a chemical composition, and compositions and sugar content.

Expert panel of fruit value pH, citric acid content the differ depending on the and sugar content. scrapped.

The melon citric acid of consigned objects of the present invention inventor produces a experiment according selecting and-sugar and compositions, it was determined that difference in species melon (table 2 reference). For example, some [...] low citric acid the melon species been it (e.g., table Lunastar of 2). Furthermore, fruit species [...] part citric acid to malic and has a relatively low non the party decision which burns sucrose to 6 revealed that is a high ratio. While, orientalis species melon, e.g. re-plaque Nice CPU checks the melon species revealed that citric acid is a high content (e.g., table YUCA of 2). NCIMB YUCA a representative (Scotland the Y which it will know[...] material 24 3 non atm) to user 17 December 2003 filed under access (access number NCIMB 41203) is YUC-15. These melon other 6 and of the citric acid to malic ratio of sucrose to service zone and deactivates both revealed that is a high. Furthermore, some Gaul species melon, e.g. MG755 method, citric has revealed that is a high content. Surprisingly, according to the present invention, and melon species re-plaque Nice[...] species melon (table 2 of Prince PF) through cross fertilization between, re-plaque Nice high citric acid high melon species other 6 and dissociated and sucrose to service zone and deactivates [...] species melon high sucrose to 6 were capable of combination with non the party decision which burns. Furthermore, the present invention refers to bis witty repartee consigned objects, e.g. [...] and IND-35 (IND-35 comparison with a [...] when. resins containing low levels of citric acid) citric acid, among other things, identifying improving edges of content.

Furthermore, per fruit melon same in both lower than the pressure pH is high without, recoat, prevented the stored quantity decreases, that no compatible even is capable of being isolated from the art despite estimated (Stepanski et al (1999)), a preferred fruit are of the present invention inventor; and the abovementioned sugar content of combining of organic acids and pH can be (table 3 reference).

Furthermore, the present invention refers to pH low background melon suite for disclosure a method for the bottled traits. Low background melon suite at eating fruit the presence of pH order to increase the concentration an acid dissociation constant of a lower the pH, a preferred pH citric acid and a mild content the combination with and concentration sugar concentration and non-greasy to the new provides taste and flavor and is thus.

In one aspect, low pH is determined by low pH gene transgenic. In one aspect, low pH gene obtained in the wild melon awareness, touring, and conferencing or consigned objects. In one aspect, low pH gene or consigned objects melon wild used as the provider awareness, touring, and a taste is preferably while producing fruit commercially is satisfactory crops crane feature facilitates configuration of the melon and comprising at the host includes a host. In one aspect, such sucrose provider such as sugar-coated possess moderate levels-accumulating properties is connected to the semiconductor layer.. In one aspect, such wild melon awareness, touring, and conferencing or consigned objects then, the result of calculation is to have fixed angle range for the one or more: non respiratory rise properties, size and of fruits relatively large middle rind component, a crunch crunch. In one aspect, low pH NCIMB is seed representative transgenic (non atm Scotland the Y which it will know[...] material 24 3) access number as 17 December 2003 to purpose: a in the NCIMB 41202 IND-35 obtained from system. In variants on the botanytoethe D is Oh species that can be classification apparatus for normalization. Melon as consigned objects, with large reason [...] (acidulus) variants (Naudin, Pitrat et al.) in. a cold water valve is shut is classification.

The citric acid present fruit family of IND-35 is an apparatus for detecting a motion organic acid (citric acid per 100g fwt 911 milligram hereinafter), is 50 milligram hereinafter per 100g fwt content malic. Surprisingly, sucrose fruit of IND-35 accumulate the build-in a concentration appropriate of properties is connected to the semiconductor layer. (100g fwt per sucrose 1.7g hereinafter, fruit deletion after late harvesting 50 or 53 is then long-term fruit 5.6g per total per 100g fwt cycles later). Table 2 the IND-35 efficacy as an anti-organizes and an analysis result of fruit, about pH 4.9 and, about 340 milligram/100g live weight (fwt) citric acid content, sugar content is about 4.0g/100g fwt.

In one aspect, low pH transgenic separated from one another with molecular markers. In one aspect, molecular markers of PCR amplified DNA fragments, e.g. SSR markers or RAPDS is marker. In one aspect, the host includes a host presence of amplified DNA fragments presence of self, or of specific allelochemicals exhibits between the absence or presence of a gene. In one aspect, the difference in length of amplified DNA fragments of specific allelochemicals exhibits presence of gene, thus of various gene of differences between the alleles to be able to distinguish. In one aspect, the present invention refers to low pH of myoblasts other to distinguish and difference between pH low in plants of disclosure the presence a marker indicating the one or more. One example of a literature such markers [Danin-Poleg et al. (2001) Theor. Appl. Genet. 102 : 61-72 and Danin-Poleg et al. (2002) Euphytica 125 : 373-384] is described CMAT 141. Other examples of the present specification to a molecular marker NE0585 NE1746 and a disclosure (reference in the embodiment 13) is connected to the semiconductor layer.. Such markers are left near pH gene constitution: this level shifter circuit consists of gene. Marker CMAT141 NE0585 and the low pH gene is present in the to one side of the, low pH gene NE1746 marker present in on the other side of the.

In one aspect, low a source a variety of the present invention inventor having pH of this marker when used primer for (segment, in the embodiment 14, table 16 reference) DNA of varying lengths it has been found that amplification of the fragments. For example, in plant (Faggous) guus pack CMAT141 for about as a result of use of the primer been amplification fragments of 176bp. PI414723 consigned objects, PI414724, than about plant PI124112 PI161375 and been amplification fragments of 175bp. , By using a time hopping code, the present specification described IND-35 plant than about 168bp and about 173bp unique fragments been a voice processing. The use of marker NE0585, and from about about 230bp are amplified in IND-35 was 232bp unique fragments thereof. The use of marker NE1746, about 127bp are amplified in IND-35 was unique fragments thereof. E.g. guus pack 124bp another of about are not in the are amplified in was IND-35 fragments thereof.

In one aspect, low pH transgenic-free plants may also the present specification described the analyzed using a primer. Low pH of member and the associated plurality of DNA fragments thereof (basic fragments) has been determined. For example, the use of of primer CMAT141 low pH than about plant-free transgenic 169bp, and from about dir 172bp 178bp detected as fragments of length. NE0585 of primer the use of, about 218bp, about 229bp, about 234bp and from about 239bp detected as fragments of length. NE1746 of primer the use of, about 133bp, about 142bp and from about 145bp detected as fragments of length. One skilled in the art such as low pH traits analyzing other plant free low pH of member and the associated other DNA fragments for measuring 2000, I know method. (Bp) size presented at has absolute has, same primer pairs detected by the relative to product of different size thereof in the following. Actual fragments amplified (accurate) size (e.g., measuring sequence analysis) the specification presented size and may vary slightly (+ /-1bp).

Therefore, in one aspect the present invention refers to the present specification described a primer using a DNA described specification to be used in amplification fragment DNA containing a sequence of melon it is pouring into a mold disclosure the plant. In one aspect CMAT141 can identify marker when is used a primer, said plant DNA from DNA fragments of about 168bp to about 178bp is a voice processing. In one aspect CMAT141 can identify marker when is used a primer 168bp, 173bp, 169bp, 172bp or 178bp of DNA fragments is a voice processing. In one aspect CMAT141 can identify marker when is used a primer DNA fragments of less than 176bp is a voice processing. In one aspect CMAT141 can identify marker when is used a primer DNA fragments of less than 175bp is a voice processing. In one aspect a primer can identify marker NE0585 system having automatic detection function of 253bp to about 218bp if DNA fragments is a voice processing. In one aspect NE0585 can identify marker when is used a primer 230bp, 232bp, 218bp, 229bp, 234bp or 239bp of DNA fragments is a voice processing. In one aspect a primer can identify marker NE1746 system having automatic detection function of 145bp to about 121bp if DNA fragments is a voice processing. In one aspect NE1746 can identify marker when is used a primer 124bp, 127bp, 133bp, 142bp or 145bp of DNA fragments is a voice processing.

In one aspect, the lower-pH traits indicative of the present invention inventor DNA fragments and low pH gene connection between separated the body. it is pouring into a mold for amplifying fragments basic in this case their low pH and is coupled to transgenic DNA sequence. Therefore, according to one aspect the present invention refers to basic alleles is indicative of an by a marker gene, in particular the present specification when used with a marker capable disclosure to simultaneous separated a low pH containing plants that disclosure the traits. In one aspect pH gene are basic marker to one side of the is. In one aspect pH gene at the sides of the are basic is a marker. In one aspect, the present disclosure to such plant of fruit specification and comprising at pH within the scope. In one aspect, the present specification to such plant of fruit as described which has a chemical composition, and compositions-sugar. In one aspect, the present fruit of such plant specification described of an organic acid such content and composition and comprising at. In one aspect, the present fruit of such plant as described to pH specification, -sugar and compositions, and comprising at and composition content of organic acids.

Therefore, in one aspect, the present invention refers to connected to low pH gene, basic it is pouring into a mold for amplifying DNA fragments, DNA containing a sequence of c. Billboard disclosure the plant. In one aspect, the present invention refers to basic it is pouring into a mold for amplifying DNA fragments containing DNA sequence and low pH gene chromosome fragments containing c. Billboard disclosure the plant. In one aspect, such chromosome fragment in plants said homo-junction-is or hetero-junction is water. Such c. The present positive examples representative plant billboard described specification is SOLAZ/2 system. CMAT141 marker in SOLAZ/2, NE0585 and NE1746 of primer the time of using a each between about 172bp, about 229bp. amplified fragments of 124bp and from about.

In one aspect, the present invention refers to low pH gene to one side of the basic DNA fragments it is pouring into a mold for amplifying DNA sequence, low pH gene and low pH gene basic in another aspect of DNA it is pouring into a mold for amplifying DNA fragments and acid sequence, these two DNA sequences of phytotoxicity in a plant coupled to said pH gene type the inventive arrangement is characterized in that. Billboard disclosure the plant. In one aspect, the present invention refers to low pH gene to one side of the basic DNA fragments it is pouring into a mold for amplifying DNA sequence, low pH gene and low pH gene basic in another aspect of it is pouring into a mold for amplifying DNA fragments containing a sequence of DNA chromosome fragments containing c. Billboard disclosure the plant. In one aspect, such chromosome fragment disparate plant said is-homo-junction or the first compound. Such representative example of plants melon the present specification is described SOLAZ/1 system. CMAT141 marker in SOLAZ/1, NE0585 and NE1746 of primer the time of using a each between about 172bp, about 239bp. amplified fragments of 142bp and from about. Such c. Other plant billboard the present representative example is system YUSOL/3 described specification. CMAT141 marker in YUSOL/3, NE0585 and NE1746 of primer the time of using a, each between about 172bp, about 239bp. amplified fragments of 145bp and from about.

Therefore, the present invention refers to low pH containing traits using plant the melon for obtaining plant melon of the present invention disclosure a use. In one aspect, low pH and the plant the melon containing traits such as sucrose in addition suitable per properties accumulates the concentration. In one aspect, low pH containing traits plant the melon the accumulation citric acid into a large amount, and. In one aspect, low pH a small amount of plant the melon containing traits accumulation a-malic acid. In one aspect, low pH containing traits IND-35 plants are the melon is or progeny plant system.

In one aspect, low pH said IND-35 obtained in the transgenic progeny system. If one skilled in the art, low pH sources even having other can be obtained, I know is will be. In one aspect, a low pH present sources such other users IND-35 the transgenic gene alleles and low pH is transgenic. In one aspect, low pH gene IND-35 is lines are examined for a low system to pH gene containing gene alleles check is for measuring the whether is embodiment. Crossing system to be inspected the IND-35 system control system are used, low pH descendant obtained therefrom is measured is splicing ratio of phenotype.

In one aspect, a plant of improved melon system IND-35 system, preferably citric acid content and per is crossing system of high concentration of. After each cross fertilization, low pH the transgenic fruit is the selection plant. Sorting the citric acid content and high sugar content respect to. the formation of interference fringes embodiment. Systematic and selected for integrating low pH transgenic embodiment relate to the disclosure in the embodiment 7 to 11.

In one aspect, the disclosure in front of molecular markers of pH a background preferably is used to deliver the transgenic. Specifically, the present specification as described to fruit of plants melon to remarkably increase the content citric acid present in used in method. In one aspect, low pH corresponding to transgenic the amplified the segment is carried out by using an acidulous in the plant.

According to one aspect, suite c. The introduced traits pH low plant billboard, such low pH of melon-free transgenic fruit, e.g. low pH in an inbred strain-free transgenic or near in an inbred strain than system adversely affect the biodegradability of the layer reduction of unit 1 to about 2.5 pH, in one aspect about 1.5 to about 2.0 pH obtained a reduction in unit. In one aspect, at eating plant melon citric acid present in said content upon incorporation of pH low plant melon, such low pH of melon-free transgenic compared to fruit, e.g. low pH in an inbred strain-free transgenic or near in an inbred strain adversely affect the biodegradability of the layer system than is gradient until 1.5 to about 3 times. Fruit maturation of plant various and compared. For example, the low pH 1B table and 1A table containing traits pH and low the melon melon-free transgenic, e.g. YSAZ X YUSOL MILENIUM-DENEV F1 and, SOLAR F1 and SOLAZ X YUSOL, L53-L53 AZ A-L53 AZ B/Mehari is a comparison of the interconnection between.

Therefore, transgenic fruit melon of the present invention a low pH (acidity can be proper)-free by weight, based on circulating.-voltage, which is substantially greater concentration organic acid than melon for comparison. PH occurring in the fruit melon of the present invention difference composition rather than concentration total of organic acids (pKa of dominant) correlate with. more. Generally increase pH can be observed during late mature sucrose with accumulation simultaneously occurs. Such organic acid content by the increase in pH is buffer. Low high content an organic acid results in a reduction perceptual sour increase and pH.. For example, an distributed rolled, both rollers are rolled in the content of melon [...] melon citric acid, such as, respiratory e.g. pH increase the melon species conversion rise 1.0pH unit (in pH 6.0 7.0) at least. , By using a time hopping code, during mature content higher of organic acids to reduce up-the pH, the that breath rise , low and frequency generating device perceptual sour such as melon lowers the inhibiting or attenuation.

Therefore, in one aspect the present invention refers to stable low pH and high citric acid content is more sour based on disclosure a stable melon fruit. In one aspect, the present invention refers to aged or after harvesting after-flavored having increased stability, melon disclosure the fruit. Furthermore, the present invention refers to plant melon hot isotropic compressing, processing, and an acid dissociation constant of fruit low pH traits comprising introducing the fruit of plants melon increase pH to a delaying or reducing the disclosure a method for. In one aspect, such method the plants properties rise respiratory or non that breath rise low pH as to introducing the traits. In one aspect, such method a low rings or unconverted plants with transgenic plants capable for integrating low pH as to.

In one aspect, the present invention refers to pH increased suitable (highest 0.5pH unit) suitable content and green based on increased skew an initial function, and waits for a taste or fruit taste scene slightly a thick a flavored taste disclosure a mature plant the melon.

According to the present invention, transgenic low pH the-sugar and compositions for producing of fruits various is introducing into the plant the melon. In one aspect such as YUCA as source per re-plaque Nice -orientalis is carried out by using an acidulous plant species. Fruit of mature fruit from chamber of moisture up to on-stream cycle time of fruits known as one elapsing after this (pollination) (cycle time) of the sucrose such as accumulation of per pending question does not increase although generally is observation. In one aspect, in the present invention a suitable sugar early for producing fruit absorb a slight shock occurring at the accumulation is carried out by using an acidulous plant the melon. For example, plant species Gaul, e.g. OGEL is used as a source of awareness, touring, and conferencing party. According to one aspect, sucrose respectively from concentration initial the party decision which burns 6 quickly at a high concentration a latent-time or can accumulate flowers, roots and Barks of the melon fruit production used in the present invention. For example, such plant of the genus [...] L53 such as sucrose build-up is used as source a latent.

Low pH of selecting transgenic examples delivering the information to the systematic and hereinafter of disclosure the embodiment aspect. Table 8 delivering systematic and a selected obtained during a melon plant is formed inside the tie. disclosure. However, other melon other of the aforementioned kind one or varieties or varieties melon used in context of the present invention may of potently plant melon the present invention according to.

According to one aspect, low pH c containing traits. Gaul one or orientalis flowers, roots and Barks of billboard c species. Billboard is crossing plant. For example, low pH gene containing c. Is system IND-35 plants are billboard. For example, c species orientalis. Billboard plants are YUCA and, Gaul as OGEL plants are melon species, is described hereinafter in the embodiment 7. Descendant which is obtained as a result of Gaul one or orientalis for example further and plant melon is cross fertilization pH preferably-sugar and and an organic acid can be obtained. Specification disclosure to the present method such as pH, organic acid and is supported by measuring per. Or, characterized is desirable of selecting plant melon of Gaul one or orientalis the specification to melon as described starting from for obtaining plant, use can be made of, in addition.

According to one aspect, low pH c containing traits. Apparatus for normalization species [...] plants are billboard. Billboard plant, e.g. in the embodiment 11 as described to can be crossing L53. Such also are melon of alternative species [...] by screening for desirable characteristics and then, the present specification to as described melon plant for obtaining can be used as starting materials.

Furthermore, type other. Low pH bell plant billboard containing traits crossing plant the melon the subject specification as described to provides plant melon.

According to one aspect, the present invention refers to plants and then shrinp fruit is maintained or fruit is characterized to be stored in a the harvested and remain stable, which are capable of producing fruit disclosure the plant. One aspect described specification read a feature of the of fruits after harvesting of fruit during the lifetime in market is stabilize. The organoleptic fruit of the present invention the features and sensation that the engine be stored for extended periods without loss of perfume can be or transporting an.

In one aspect, is of fruits has pH full ripeness of fruit of the present invention after reaching the is stabilize. In one aspect, the present invention according to is of fruits has pH of fruit after reaching the full ripeness about 4.2 to about 5.6 is to maintain. In one aspect, the present invention according to fruit citrate full ripeness is fruit content after reaching the is stabilize. In one aspect, the present invention according to fruit citrate content full ripeness end after reaching the fruit is being maintained above 400 milligram per 100g fwt. In one aspect, the present invention according to fruit of malic full ripeness is fruit content after reaching the is stabilize. In one aspect, the present invention according to the ratio of malic to citrate fruit full ripeness is fruit after reaching the remains of the in a stable state. In one aspect, the ratio of citric acid to malic full ripeness is fruit after reaching the remains of the high specific than 4.4. In one aspect, these features are fruit is full ripeness end after reaching the present specification is described to maintain. In one aspect, these features are fruit is full ripeness application about of feature values is measured when the probe 70 to about 130% within range is maintained at a, in one aspect fruit is full ripeness application about of feature values is measured when the probe 80 to about 120% within range is grown on the substrate positioned in, in one aspect fruit is full ripeness measurement value is measured when the probe application about 90 to about 110% within range block 7 is slid as is. According to one aspect, the present invention refers to such features are fruit is full ripeness end after reaching the plant when the smart memory module is present, or harvested stored that is maintained in an, which are capable of producing fruit disclosure the plant. In one aspect, the present invention refers to fruit these plants least such feature does provide when the smart memory module is present that is maintained in stable during 2, in one aspect fruit these plants at least when the smart memory module is present that is maintained in stable during 3, in one aspect fruit these plants at least when the smart memory module is present that is maintained in stable during 4, which are capable of producing fruit disclosure the plant. According to one aspect, the present invention refers to such features are to be stored in a 20 °C during at least 5, in one aspect 20 °C be stored, in during at least 7, at least be stored, in 20 °C 9 that is maintained in stable during, which are capable of producing fruit disclosure the plant. According to one aspect, the present invention refers to such features are 8 to 12 °C be stored, in then during at least 7 at least 20 °C during 2, according to one aspect, at least 8 to 12 °C at least be stored, in then during 20 °C 12 during at least 2, according to one aspect 8 to 12 °C 26 at least be stored, in at least 20 °C then during that is maintained in stable during 2, which are capable of producing fruit disclosure the plant. Typically, fruit harvesting constitution described specification conditions several hours until be stored, in environmental to be maintained at opening.

The present invention according to these plants fruit of plants when the smart memory module is present in the embodiment 15 examples evolution of features indicative of, table 17 presented of wet liquid to flow down. The present invention according to to be stored in a fruit of plants after harvesting is indicative examples evolution of features in the embodiment 16, 19 and 18 table presented of wet liquid to flow down.

In one aspect, the lung are melon (SSL) shelf life, shelf life or organs (MSL) shelf life concentrator can be classification as (LSL). Millennium examples of melon LSL, saponites-PLC, is LSL [...] unconverted and from a. Gaul examples of melon MSL is [...] and MSL. Shelf life groups examples of melon in (SSL) is a [...]. Typically, in plants of fruit melon SSL about lifetime is 1 to about 2. This in order to avoid loss about fruit harvested each 2 to 1 which implies that the should.. Typically, in plants of fruit melon MSL about lifetime is 3 to about 4. This fruit in order to avoid loss about 3 to 4 should each. which implies that the harvested. Typically, in plants of fruit melon LSL about lifetime is greater than or equal to 5. Typically, 8 to 12 °C the melon SSL about 4 to about 7 in the 20 °C followed by a during about 20 °C during or 2 can be of long-term storage 4 to 3. Typically, 8 to 12 °C the melon MSL about 7 to about 12 in the 20 °C followed by a during during 2, or 20 °C about 5 to 10 can be of long-term storage. Typically, 8 to 12 °C the melon LSL about 12 during 20 °C followed by a 2 in the least, or about 20 °C 10 can be of long-term storage or more.

According to one aspect, of the present invention plant long-term shelf life (MSL) concentrator or fruit of shelf life (LSL) capable of producing fruit of..

The lower-of the present invention inventor pH citric acid and high transgenic full ripeness is fruit content in fruit melon end after reaching the, respiratory in melon rise respiratory conversion and in particular accelerated rise has been observed on that. This rapid growth in content malic and pH e.g. and green content is ascertained through reduction of decomposition of fruit as guided. Furthermore, a white-a accelerating said composed of the powder of I appearance of decompositon acid and an alcohol tissue also identified. Conversion in the case of melon synergistic and respiratory melon fruit decreases while is shelf life of, this phenomenon is unconverted and/or non respiratory rise not identified in melon. Therefore, in one aspect, gene ring low or unconverted and the plant of the present invention is melon species. According to one aspect, lift or respiratory a non plant of the present invention capable of producing fruit that breath rise..

Therefore, in one aspect the present invention refers to low pH gene containing the property rise respiratory or non that breath rise c. Billboard disclosure the plant. In one aspect, such melon fruit of plants described specification further features of and compositions sugar content and comprising at. In one aspect, such melon fruit of plants described specification further features of and compositions content organic acids and comprising at. According to one aspect, the present specification to such melon fruit of plants as described and comprising at ratio of the malic to citric acid. In one aspect, such plant of fruit further as described to specification and comprising at pH.

In one aspect, the present invention refers to low rings or unconverted at low pH gene capable of producing fruit containing c. Billboard disclosure the plant. In one aspect, such c. Fruit of plants billboard described specification further for sugar content and comprising at features. In one aspect, such c. Billboard specification further fruit of plants described organic acids and comprising at feature for content. In one aspect, the present specification to such melon fruit of plants as described and comprising at ratio of the malic to citric acid. In one aspect, such plant of fruit further as described to specification and comprising at pH.

In one aspect, the melon fruit body of fruit of the present invention inventor are identified in a low pH is orange pulp very of fruits having color strength is associated with a poor identifying improving. Specifically, for producing fruit index this orange fruit when the plant the melon weak orange or revealed that color is orange sides of the channel region (table 4 reference). Surprisingly, the melon fruit of plants of the present invention inventor low pH and a thick orange may incorporate color we have found that.

Table MEHARI-based hybrid [...] the 4 a the parent of that mutant fruit system L53 the color strength thick or strongly thick orange (average 5.17) are indicative of of wet liquid to flow down. L53 low pH for introducing transgenic backcrossing program generated plant-free transgenic low pH (pH high L53*)pH color strength and reduced slightly (6.46 to 4.71) exhibit. Said backcrossing program is generated in low pH color strength and the plants containing traits additional pH exhibit reduced. Homo-junction body state plant having low pH traits (L53 * low pH) is orange fruit body color of the lowest rated and ratings was 4.04. Hetero-junction body state plant having low pH traits (L53 * acid) is orange fruit body color of was 4.36 medium rated and ratings. Low pH and a relation between color at eating is orange sides of the channel region or phenotypic effects of. suggesting a. Therefore, low pH at eating orange a strong even presence of transgenic plant color has proof-reading activity (one aspect either at a later time in described scale in 4 or more grade, in one aspect 5 grade, in one aspect 6 grade).

In one aspect, the present invention refers to pH about 4.5 to about 5.6, which are capable of producing fruit in the existing ways to melon, fruit pulps of orange rated and ratings disclosure a plant melon or more 4. In one aspect, the colored orange of fruit at least 5 rated and ratings, in one aspect is 6. In one aspect, said fruit of about pH 4.5 to about 5.4 and, in one aspect is about 4.8 to about 5.2. In one aspect, about citric acid per 100g fwt said fruit containing a 900 milligram to about 400 milligram. In one aspect, said fruit about 450 milligram to about 750 milligram per 100g fwt containing the citric acid. In one aspect, said fruit approximately 100g fwt 5g or more sugars, in one aspect of approximately 100g fwt 13.0g to about 5.0g. containing fermentable sugars. In one aspect, 12.0g to about 5.0 approximately 100g fwt fruit of. containing fermentable sugars. In one aspect, the ratio of said fruit citrate to malic range about 4 to 30, in one aspect ranging from about 5 to about 15. In one aspect, present in the fruit of plants said sucrose to 6 is the party decision which burns ratio of about 1:1 to about 1:2.

Color grade scale as follows: 1; white, 2 ; weak orange, 3 ; sides of the channel region is orange color, 4 ; orange, 5 ; thick orange, 6 ; very thick orange (table 4 reference). In one aspect, and a name of the fruit such as spectrophotometer centimeter -2500d american glow other be measured by use of a spectrophotometer.

In one aspect, the present invention according to a feature of the fruit melon full ripeness harvested in of fruits, i.e. has measure the mature fruit. Melon fruit of the specific compositions, according to taste is harvested mature in the affected. Melon fruit during sugars and is process dynamic accumulation organic acid. Fruit is full ripeness per the sooner, the to is disclosure which decreases accumulation of and an organic acid. When process mature, sugars and organic acid has a predetermined decomposition accumulation, or specific time each conforming a pattern, panel environment and affected by growth conditions. If one skilled in the art, mature fruit melon and method determining that fruit melon shrinp of indicating a criterion is will be know.

In one aspect, then, the extracellularly mature markers of mature melon suite one is used to identify a:

Aging leaf fruit-side (melon fruit a leaf flower stalk configured on an insertion part for leaf armpit flower fruit side lobe by chamber in. is referred). Is-necrosis the leaves fruit side.

-Peel color change (Gaul species in green in film has a yellow, [...] species in gray in cream light film has a yellow, or increase in component yellow in species saponites-PLC).

- flower stalk ten ([...] melon in and, [...] , Gaul in do not go).

-Peel lowering of rigid, in particular flower in terminal portions.

In one aspect, "shrinp" the "initial-partially matured dendritic cells" to "2", "later-complete mature" to "4" between step distinguished by. mature physiologically. In one aspect, melon initial-partially matured dendritic cells sucrose content accumulation initial sucrose step 2g per 100g fwt. or more. In one aspect, this citric acid and reducing sugar (glucose + oligosaccharides) of the maximum of the content, and typical fruit mature relates to at eating pigment and tissue. In one aspect, sucrose and objects, such that highest step mature complete-for the post-recording of melon endpoint content is defined. Furthermore, before disclosure of the electrolysis process, e.g. fruit at eating hardness of loss, glucose, citric acid content of the deterioration, or malic is defined as content. Therefore, according to one aspect, the fruit of sucrose content of fruit per 100g fwt shrinp 2g is reached is disclosure. In one aspect, the increase in sucrose content shrinp longer. continues until not observed.

The present specification disclosure to measurements is generally fruit plurality of irradiation from. an average of measurements or data. sour hour blood know, each of plants are fruit of vegetable-derived or each fruit which of the sample during the growth of the plant melon whether a change used to monitor since described are a to give not go to the terminal, referring to a. In one aspect, the present specification described melon fruit a feature of the present specification under conditions or similar conditions described culturing of fruits detected by using the a (e.g., hereinafter in the embodiment 6, table 5-7 reference). In one aspect, a plastic in the present invention according to feature values of plant cultivation richer film pile fruit is a measured average value in (pile grown in plant in the case of, one fruit per plant).

In one aspect, in an inbred strain and the plant of the present invention, high-or hybrid number field wear under high vacuum. In one aspect, pH low systems in an inbred strain specification this transgenic described organic acids, and comprising at pH from a sugar and features of. At this time, said plant melon other systems in an inbred strain, preferably other in an inbred strain is exemplified by cross fertilization provides plant hybrid the present invention according to. In one aspect, other cross fertilization citric acid also systems in an inbred strain a high sugar content and/or content capable of producing fruit.. The present invention according to a representative in an inbred strain systems are as a disclosure to 1A table.

In one aspect, is plant hybrid plants are of the present invention. At this time, low pH, high citric acid content and high sugar content in which a feature such as a parent part of either is provided from at example, a parental, microetch is provided example, a parental remaining other. According to one aspect, a cross fertilization parents high citric acid content, pH but high sugar content (e.g., about pH 6.5) and having high water production while fruit, parents other cross fertilization fruit low pH are produced. According to one aspect, the fruit example, a parental other citric acid. and has high content. Representative hybrid ideographic 1B to the present invention according to the disclosure. As used in the present specification, the terms "plant" rotation plant cells, plant protoplasts, melon plant tissue may be played of a culture plant cells, plant callus, plant thicket , and plant or plant parts, e.g. flowerpot, flower, leaf, stem. plant cells derived from. In one aspect, plants are edible and of the present invention capable of producing fruit melon.. (At eating) middle rind a melon fruit edible portion of blades, presenting a. As seeds middle rind around steel, peel (or coat) side is enclosed.. The present invention according to is greater than 2 centimeter are middle rind of fruit, preferably total fruit live %, by weight of the water preferably the user which exceeds a preset 50%. In one aspect, the present invention according to melon fruit of plants at eating of green, white, yellow, or orange is.

In one aspect, the present invention refers to the present invention according to useful when for culturing plant tissue provides regenerative cells. The present invention according to plant characteristics of tissue culture plants. capable of reproducing. Such tissues used culture embryonic function, and waits for a regenerative cells, protoplasts, mitotic tissue cells, callus, flowerpot, leaves, capsular flowerpot, root, root end or flower is preferably not less. The, the present invention refers to in addition the present invention according to tissue culture to be reproduced from that provides plant melon. Reproducing method is US 6,420,631 to examples of disclosure. Furthermore, the present invention refers to the present invention according to a step of collecting, tissue of plants, collected tissue is incubated to allow a breeding obtain and grow sprouts, the water for the buds beforehand by removing foreign matters by and Young pak root like from biological samples plant including obtain, asexual plant of the present invention provides a method. Furthermore, the present invention refers to the present invention according to obtain plant, or of self-such plant of such plant and other melon step and crossing the plant, seed from which the descendant and includes harvesting the, seed from which the plant grows producing method of the present invention according to the disclosure. Furthermore, the present invention refers to the present invention according to step of embedding plant, such plant growing the specification described features containing fruit including step of harvesting fruit production method of the disclosure. Furthermore, the present invention refers to the present specification as described to said fruit and storing the upper unit further includes. Furthermore, the present invention refers to said fruit the upper unit further includes transporting the. In one aspect, the present specification described said fruit features of of said fruit is stabilize during storage. In one aspect, the present specification described said fruit features of of said fruit is stabilize during storage.

Plurality of melon several of renewal and lineage species characterized as follows. These features are various melon example of system species, various melon species is not defining a plurality of lines by a rope. and illustrated. Therefore, these features or inorganic there may.

[...] species melon:

Fruit: the type disadvantageous with respect to circular to circular; 1200grs to 600 size; peel: color ashing a line is stipendiary gray , , smooth envelope surface and is (smooth or slightly 56b shell). Orange, spacing, a sweet a very which nevertheless is strong in aroma, respiratory synergistic. Some respiratory variants [...] color peel is reduced step rise does not are switched.

Variants LUNASTAR: a star base or[...] species derived from (Nunhem) heme's eye is hybrid of monoecism.

re-plaque Nice species melon:

Fruit: the type disadvantageous with respect to circular to circular; 1500grs to 700 size; peel: ashing color 56b shell (used for displaying an opening the roller which becomes neutralized specific). stipendiary yellow (specific orange), a spacing, to crunch crunch very sweet (low flavor) (only some cases), period to a respiratory synergistic.

Variants YUCA: re-plaque Niceshipmate of positive male of hybrid of at eating green species.

Variants PRINCE: re-plaque Niceshipmate of positive male of at eating orange species of hybrid.

Gaul species melon:

Fruit: the type disadvantageous with respect to circular to circular; 1500grs to 600 size; peel: green conversion yellow 56b shell. A good sweet stipendiary white spacing, a flavored. Concentrator having a short period are shelf life, respiratory synergistic.

Milligram. 755 variants: shipmate of positive male of Gaul of hybrid. Fruit [...] within 26.66. high and very short period.

Melon species saponites-PLC:

Fruit: oval to rectangular-oval form; 2 size 5Kgrs to; peel: improvement of skin diseases, Vaginitis green spots and several longitudinal 56b shell. Many white crunch crunch and flavor of the juice and a sweet. Intermediate fruit period, long-term storage life, non respiratory synergistic.

Variants Sancho: shipmate of positive male species saponites-PLC of hybrid. Polysilicon shell, high [...] value.

guus green onion species melon:

Fruit: usually short cylinder-like shape, ; size 1000grs to 300 ; peel: green shell. A sweet white crunch crunch at eating not. In salad green for consumption. monoecism.

IND 35 :

Fruit: a back to form oval; size 300 to 1000g; peel: sheathing, which is point green bright, yellow upon shrinp. The moon and white crunch crunch at eating. In salad green for consumption. monoecism. Syngenta seed system, non respiratory synergistic.

YUSOL: the type disadvantageous with respect to circular to circular; 1300grs to 600 size; peel: how many mesh for rope-green bright yellow shell. A flavored stipendiary white spacing, an acid-free properties, and hardly sweet fruit body. Shelf life the metaphase of shorter period. that breath rise characteristic non. Syngenta of shipmate of positive male seed system.

YUSAZ A: the type disadvantageous with respect to circular to circular; 1300grs to 600 size; peel: net and line is stipendiary gray properties, and hardly ashing color shell. Green in particular properties, and hardly perfume the crunch crunch with sweet acidic fruit body. mid-long term and short period are shelf life. Non, in lake to that breath rise characteristicsuction monk characteristic. Syngenta of shipmate of positive male seed system.

YUSAZ B: the type disadvantageous with respect to circular to circular; 1300grs to 600 size; peel: a line is stipendiary gray 56b yellow shell. Green in particular properties, and hardly perfume the crunch crunch with sweet acidic fruit body. A long time cycle, and to shelf life, non, that breath rise characteristic. Syngenta of shipmate of positive male seed system.

MILENIUM-DENEV: the type disadvantageous with respect to circular to circular; 1500grs to 600 size; peel: line is free 56b yellow shell. stipendiary gray of and free from perfume the crunch crunch a sweet non-acidic. Long and period, non, long-term storage life, non respiratory synergistic. Syngenta shipmate of positive male of hybrid seed.

SOLAZ/1: the type disadvantageous with respect to circular to circular; 1400grs to 700 size; peel: net shape green bright line is and properties, and hardly yellow shell. White crunch crunch acidic with sweet free flavor for most of the fruit body. Use of which a long-life and storing short period are, unconverted, very little respiratory synergistic. Syngenta of shipmate of positive male seed system.

SOLAZ/2: the type disadvantageous with respect to circular to circular; 1300grs to 600 size; peel: net and of strip-green bright properties, and hardly yellow shell. White crunch crunch acidic with sweet properties, and hardly perfume the fruit body. And short period are, long-term storage life, non, very little respiratory synergistic. Syngenta of shipmate of positive male seed system.

MEHARI: [...] belonging to the genus of Syngenta monoecism hybrid. conversion characteristic , concentrator shelf life.

L53: [...] belonging to the genus of positive male of Syngenta shipmate of systems. conversion characteristic , concentrator shelf life. Small size and low round flat grudge form. conversion characteristic[...] Nunhem belonging to the genus derived from results in varieties is hybrid of monoecism of LUNASTAR.

TD: [...] belonging to the genus of Syngenta monoecism of systems. Non, long-term storage life. Non Limagrain [...] belonging to the genus derived from hybrid of monoecism TORNADO results in varieties is derived from.

The present specification all reference literature citation in the specialized been citation referred the present application. The present specification to the regions may be disclosure, these disc spin speed value within a range substantially any angle level should is considered portion of the present invention.

Applications of the present invention relate hereinafter embodiment describes a should regarded as. Hereinafter embodiment of the present invention relate, so as to define an totally range or limited is don't is declared to be.

In the embodiment 1: melon of processing for extracts of

Melon fruit for taking the results of a about from 400g of wedge-like fragments, seeds peel (1 centimeter thickness) and removed the. Sever a small matrix shape, soft [...] until a slurry is attained in blender was blended (Warring) 30 seconds. Only watt slurry wherein the filtration of the slurry suspension using connected with ADSL, VDSL, the path 10,000g [...] , in a centrifuge in man stored up to -20 ° C flavor which has a large alcohol content.

In the embodiment 2: measurement of content citric acid

In the embodiment as produced in sample number citrate LYASE (citrate lyase: CL) and a with acetate and oxalate citric acid into the incubating the converted into acetate. Maleate dehydrogenase (MDH) and lactate dehydrogenase (LDH) in enzyme such as, with acetate and oxalate NADH pyruvates are oxygenated derivatives decarburisation thereof by and end L L-lactate to reduction. Citric acid in a sample a reduction of the NADH to the amount of proportional to the enlargement, .can be measured in the 340 nanometer. The analysis performed for all the micro plate. L micro 20 sample dilution micro plate for added. This reputation to NADH, MDH and LDH containing an analyte mixture mixed by adding l micro 200. Reaction is CL l micro 15 containing starter solution effect by adding of been disclosure. Mixing flat plate are the reactor and which is directed was the composition is left for time 1. Microtiter plates reader (Bioteck EL808 reader, attached to computer software and KCJunior) it was determined that absorbance in 340 nanometer to. Correction (calibration) obtained by using the bill concentration citric acid in a sample. For purchasing a in [...] die [...] enzyme (Roche Diagnostics).

In the embodiment 3: malic content reading

In the embodiment 1 as produced in sample L-maleate dehydrogenase (MDH) and NAD⁺ and a the incubating the with acetate oxalate rate end L the conversion. The reaction equilibrium but the maleate on the front side, hydrazine using derivatives via the traveling oxaloacetate. NADH formed during the this analysis a material present in the. proportional to content malic. Therefore, as a measure of malic the NADH can be measured in the 340 nanometer.. The analysis performed for all the micro plate. L micro 20 sample dilution micro plate for added. This reputation to hydrazine, MDH and NAD⁺ containing an analyte mixture (pH 10) 200 micro l to allow reaction to adding the disclosure. 1 reaction solution which is prepared by mixing flat the progress time. Microtiter plates reader (Bioteck EL808 reader, attached to computer software and KCJunior) it was determined that absorbance in 340 nanometer to. Correction (calibration) in a sample obtained by using the concentration malic the bill. The MDH [...] die [...] for purchasing a in (Roche Diagnostics).

In the embodiment 4: glucose, oligosaccharides and sucrose content reading

Glucose:

Glucose the glucose-6-phosphate dehydrogenase (G-6-PDH) [...] it was determined that using. The glucose-6-phosphate to glucose by [...] (G-6-P) phosphoric acid and, then PDH-G-6 and NADP (reaction 1 and 2) with the aid of force 6-been dehydration [...]. (H-receptor) formed the concentration of NADPH initial glucose concentration and the quantitative relationship bar, during optical spectrum of measured at 340 nanometer UV range.

Oligosaccharides:

Oligosaccharides factor have been measured by assays same. Fructose-6-phosphate by the enzyme oligosaccharides [...] (F-6-P) to been phosphorylated. F-6-P the enzyme phosphor glucose isomerase (PGI) is then converted to G-6-P by, then said reaction 2 as described 6-PG been converted into.

Sucrose:

Sucrose the enzyme β- [...] been converting oligosaccharides as glucose by. Human glucose forming the aforementioned reaction 1 and 2 has been determined according to. Corresponding advertisement based on the shown list performed in microtiter plates measurements, is affectable microtiter plates reader (Bioteck ELx808 reader, data collection software and attached to computer) is measured with. For purchasing a in [...] die [...] enzyme.

In the embodiment 5:pH measuring

In the embodiment 1 as described above to a standard solution of nucleic acid pH pH 7 to pH 4 and CRIMSON GLP21 pH it was determined that the measurer is used.

In the embodiment 6: melon plant cultivation conditions

Melon plants are is etched under various conditions in several places and recognizing was cultivated according to (reference 5 table hereinafter). The results of tables 6 to corresponding advertisement based on the shown arrangement, each kit article ideographic culture conditions is've got the to 7.

In the embodiment 7: systematic and selected delivery of transgenic low pH

1. IND-35 group of lines and sorting Syngenta El Ejido the cross fertilization in farm experiment (Spain).

Systematic and selected as follows:

A)-YUCA-15: period (fruit chamber and the time required until mature fruit from) is slightly long sugar a vacuum a high accumulation capacity. The YUCA of self-ownership obtained through one time 5 a is system seed [...]. This longitudinally melon CPU checks re-plaque Nice , non. ultra low synergistic respiratory is.

B)-OGEL-17: period (fruit chamber and the time required until mature fruit from) is slightly short since the slightly higher capacity accumulation sugar a vacuum. A commercial system is hybrid GUSTAL and RADICAL ([...] seed) through cross fertilization between system the parent of that mutant F1 generation from one time 10 of self-ownership obtained via a [...] seed is system. This as species melon Gaul, conversion characteristic and normal respiratory synergistic is connected to the semiconductor layer..

F1 F2 a of self-through cross fertilization into progeny are obtained. F2 fruit per and a low pH (table 8A) by screening for the.

The culturing the population of 2. F 2, a newly selected plant to plant and sorting [...] catabolic as the moisture.

A). -IND35/YUCA-15 F2 populations on a commercial hybrid BETULO (Syngenta Seeds) of self-times for 5 a ownership obtained a system seed [...]milligram. 755-68 (755) and a the cross fertilization. This Gaul conversion characteristicbell and melon synergistic respiratory and green at eating, high sugar, high content of and relatively shorter period since the citric acid is selected for particular application purpose.

B). -IND35/OGEL-17 SEN19C8 (SN8) the population of crossing the F2. EARLS SEINU hybrid folding commercial system is (Yae Nogey Seeds Co. Isahaya, Nagasaki, Japan) for 6 times of self-ownership obtained as a [...] seed system, ultra low synergistic respiratory and non.. High sugar content and net connected to the aim a selected re-plaque Nice. paper melon CPU checks.

Such populations in low pH for a dominant single gene control been identified, any between said with accumulation per transgenic low pH also association was not observed (table 3). Each populations each high degree of R (each plant is 150). I. 330 [...] recombinant containing the lowest pH was deaminase. Catabolic moisture developing seeds in was collecting offspring. F2 populations a) selected from the progeny are cross fertilization 755YUCIND-19, 755YUCIND-49, 755YUCIND-75 comprises plant represented by. F2 populations b) is selected from the descendant cross fertilization 1 SN8OGLIND-03 comprises plant that is represented as.

755YUCIND the extractor extracts accumulation sucrose lines. SN8OGLIND has the extractor extracts accumulation method, citric. Such crossing the growing the progeny are pH, and an organic acid per was collecting data on content. Sugar high, low pH and high citric acid content has proof-reading activity systematic and combination. Tables and an analysis result of plant selected 8B've got the to.

Table 8: intermediates analysis

In the embodiment 8: SOLAZ transgenic to gene introduced

Low pH traits, and is rounded and shaped at eating the white line is yellow enclosure non respiratory synergistic LSL melon to the introduced gene.

1. Selected SN8OGLIND-103 descendant plant, a moisture natural traditional Makuwauri EIJYU variants re-plaque Nice (Nanto seed Co. Ltd. , Kashiwara, Nara, Japan) through reproductive of self-from a ownership obtained seed [...] the cross fertilization and a IOTYU system. Is and synergistic respiratory non reason for this is that the of selecting, is non, having very high sugar content, citric acid content very high, is therefore casings with yellow.

2. That has been obtained in front of cross fertilization offspring of selecting plant which has an, commercial hybrid SOLARKING F1 (Nunhems Zaden BV, Haelen, Holland) in 5 of self-ownership obtained through reproductive times a [...] the cross fertilization and a SOLAR-19 system seed. And synergistic respiratory non this, very high sugar content, having lines 56b and yellow fruit body and white, the purposes used long-term storage life and. paper melon Gaul indicative of non.

Moisture self from descendant such crossing the embodiment 7 times. For fixing a and of selecting progeny of self-pH, and an organic acid per the analysis of the content was collecting data (table 8D and 8C). Progeny systematic and fixing has proof-reading activity:

A) SOLAZ 1, high sugar accumulation, germ percentages as low pH by selected together resistance, summer growth adaptive plant (F and 8E table).

B) SOLAZ 2, together with accumulation per intermediate low pH by selected as, spring growth adaptive plant (F and 8E table).

3. Descendant SOLAZ1 SOLAZ2 and the origin and ownership as the prior a [...] YUCA-15 x SOLAR-48 obtained from cross fertilization of a system comprises an improved hybrid MD.997F1 of reproductive times 6 of self-ownership obtained through a [...] seed system, crossing a YUSOL 1, 2 and 3 the moisture-managing provider been used as.

Next F1 combination are obtained:

A) YUSOL 1/SOLAZ 1 ; YUSOL1 1 family of moisture provider as when using SOLAZ 1.

B) YUSOL 2/SOLAZ 1 ; YUSOL 2 family of moisture provider as when using SOLAZ 1.

C) YUSOL 3/SOLAZ 2 ; YUSOL 3 family of moisture provider as when using a SOLAZ 2.

Plants and selected and an available commercially and an analysis result of the control group are illustrated in tables 9 and 10. A plant cultivation conditions described in the embodiment 6 the embodiment. Each experiment number of of fruits is examined under provided (nr Frt). Measurements as described 5 in the embodiment 1 to the embodiment. Sucrose (suc), (hex) and total sugar-service zone and deactivates other 6 digits for biopolymer 100g is g per (fwt) amount. 100g digits for malic and citric acid biopolymer is milligram per (fwt) amount.

In the embodiment 9: introduced gene to transform into YUSAZ A.

Low pH, and is rounded and shaped traits lines having a line is yellow green at eating non respiratory synergistic LSL melon to the introduced gene.

1. Milligram. 755-68 of the prior used as parent recursive characteristic 755YUCIND-19 system as well as directly reverse interbreeding the embodiment.

Obtained from cross fertilization front of descendant of selecting plant which has an, the prior (sister line) system sisters 5 of self-from cognate as reproductive times a ownership obtained through a system seed [...] YUCA-64 and a the cross fertilization.

Is at eating reason for this is that the selection of lines and green color, non non respiratory synergistic, long-shelf life exhibit, and highly have very high sugar crunch crunch a line is tissue and watermelon the do shell.

Such crossing of self-using offspring embodiment the one time 3. The fastenings and of selecting progeny of self-pH, and an organic acid to form the photoresist content per using the embodiment. (Table 8C-E).

2. Prior plant selected from offspring obtained, a ownership described prior seed [...] YUSOL 3 crossing the a system. At eating reason for this is that the of selecting system is green to and cyclical which has a short, having very high sugar content, .this is because the line is rind and yellow.

Descendant cross fertilization front of the embodiment of self-from one time 5. At eating and short period are at descendant of self-is green to yellow tissue and watermelon the crunch crunch lines retention offspring breeding and the fixing by screening for his writing process. Sorting and for fixing a pH, and an organic acid per auxiliary sets of analyzed data by content has. And YUSAZ A is descendant systematic and fixing in the is.

3. Ownership described prior lines YUSAZ A a [...] seed system a ownership of 3 in YUSOL1 [...] seed moisture male for crossing system used as an provider. F1 combination: YUSOL 1/YUSAZ A been are obtained.

Selected plant and an analysis result of and are illustrated in tables 11. Plant described in the embodiment 6 the grown in conditions. The number of of fruits is examined under each experiment (nr. Frt) have been shown to. Measurements as described 5 in the embodiment 1 to the embodiment. Sucrose (suc), (hex) and total sugar-service zone and deactivates other 6 digits for biopolymer 100g is g per (fwt) amount. 100g digits for malic and citric acid biopolymer is milligram per (fwt) amount.

In the embodiment 10: introduced gene to transform into YUSAZ B.

Low pH traits line is yellow, and is rounded and shaped shell be at eating green having a synergistic respiratory of the introduced gene to melon LSL.

1. Descendant of selecting the prior SN8OGLIND-103 x IOTYU cross fertilization is non plant which has an ultra low synergistic respiratory having melon the green one matrix shape (table 8D) and it has a specific distance about he backcrossing.

Said cross fertilization obtained from offspring of selecting plant which has an, the prior and sisters from cognate 5 of self-ownership obtained through reproductive times a [...] seed (table 8E) crossing the YUCA-40 system.

Is system of selecting reason for this is that the fruit is synergistic respiratory and non green to, lever is rotatably supported by the shelf life, having very high sugar content, very crunch crunch line is tissue and watermelon the. since.

2. Such system from 1 times of self-offspring obtained after prior plant which has an of selecting a ownership described crossing the seed [...] YUSOL3 system. At eating reason for this is that the of selecting system is and green to, period is further shortened, having very high sugar content, .this is because the line is rind and yellow (table 8F reference).

Descendant cross fertilization front of the embodiment of self-from one time 5. At eating at descendant of self- crunch crunch and green to citric acid and high watermelon the lines and yellow content by screening for the retention offspring.

3. Sorting and for fixing a pH, and an organic acid per auxiliary sets of analyzed data by content has. YUSAZ B is descendant systematic and fixing been is (table 12).

YUSAZ B the "+ citric acid" is one example of plant melon.

Ownership described provide the system YUSAZ B a [...] seed system ownership of 3 a [...] YUSOL 1 of seed moisture male for crossing system used as an provider. F1 combination YUSOL 1/YUSAZ B been are obtained (table 12).

Plant described in the embodiment 6 the grown in conditions. Digits of of fruits is examined under each experiment (nr. Frt) have been shown to. Measurements as described 5 in the embodiment 1 to the embodiment. Sucrose (suc), (hex) and total sugar-service zone and deactivates other 6 digits for biopolymer 100g is g per (fwt) amount. 100g digits for malic and citric acid biopolymer is milligram per (fwt) amount.

In the embodiment 11: introduced gene to transform into MEHARI F1.

Low pH at eating orange traits in melon of the introduced gene. MEHARI hybrid commercial targets for the selected ([...] seed) is 2 is a low pH variants, one a high citric acid content low, another, been converting variants with low cobalt content citric acid.

Direct from lines 755 YUCIND-75 and 1 . 755YUCI ND-49 a ownership reverse interbreedingrecursive characteristic a seed [...] L53 embodiment using the parent system. L53 sugar high, low citric acid content to this end, the. paper melon [...] selected purposes. L53 MEHARI hybrid commercially (table 8F reference) is the parent of that mutant.

Backcrossing low during the course of each having the detecta + e pH in progeny plant cross fertilization, L53 recursive characteristic train nearer to a reactive force is in addition to other transgenic perceptual acid (citric acid having high or lower) representing a descendant has proof-reading activity.

Number 2 backcrossing (3 difference cross fertilization) been is obtained next descendant such as:

A) low pH and low citric acid respect to the contents selected L53) 3) x755YUCIND75-15/03/06 /.

B) low pH and high citric acid respect to the contents selected L53) 3+)x755YUCIND49-8/2/03/05/

After such crossing of self-descendant from one time backcrossing 1 and an additional L53 4 times the embodiment. Breeding and fixing a selective descendant of self-pH process, and an organic acid to form the photoresist content per by was of support.

2. Been the selection progeny are systematic and fixing:

the populations A) L53AZ A a) been selected from, low citric acid content and low pH, and any similar to L53 recursive characteristic having fixed.

the populations B) L53AZ B b) been selected from, low pH and high citric acid content, and any similar to L53 recursive characteristic having fixed.

Furthermore, low pH at eating orange and rigid having be plants color been the selection. The intensity of orange fruit body of generally low pH at eating fruit the acid orange poor in color strength is associated is particularly tend to sorting conditions two electrodes (table 4 reference).

Descendant system for L53 system and a L53AZ B and L53AZ A MEHARI other used the crossing system example, a parental MEHARI AZ A, a board is provided to conveniently put and MEHARI AZ B each MEHARI F1 are obtained.

All of these product, system, and using the experiments in crops cranehybrid of analyte from each fruit was collecting result (table 13).

A plant cultivation conditions described in the embodiment 6 the embodiment. Digits of of fruits is examined under each experiment (nr. Frt) have been shown to. Measurements as described 5 in the embodiment 1 to the embodiment. Sucrose (suc), (hex) and total sugar-service zone and deactivates other 6 digits for biopolymer 100g is g per (fwt) amount. 100g digits for malic and citric acid biopolymer is milligram per (fwt) amount.

In the embodiment 12: functional analysis

Functional to fruit of plants the present invention according to the expert analysis the embodiment. The one such panel 12 is configured name which have, in particular tissue melon, taste acid taste and per describing is trained people. After ruling out gastric embodiment step 6 training expert step facing the rear side of the main unit. The present research in training program as follows:

-trained 1: read vocabularies, taste. In Conference room 6 match word and described, which include recording products for viewing discussed the difficulty of decryption.

-trained recording product 2:2 + panel direct techniques for viewing manner in which the evaluation words at analysis functional 2 iterations.

3-trained: training 2 provides to panel results of recording product. 2 + 2. and discussed in a Conference room. Each cabin in recording product 2.

4-trained: various concentration of sugar solution and an acid-poor region training solution a technology-word the evaluation degree of difficulty in the panel. Training 3 provides to panel results of recording product. 3 + in Conference room 1 + recording product. 3 discussed and iterations in each cabin 1 iterations.

5-trained: 4 in Conference room for recording and product 2 product recording + 2 iterations in each cabin. Taste taster between the base plate and the one is here is irradiation capacity.

-trained 6:3 in Conference room for recording and product 2 product recording + each cabin in the one taste taster 2 a irradiates the ability between the base plate and the.

Air conditioning and cabin individual analysis functional device is in a functional analysis laboratory grade configured technique, and decision point. 10 the embodiment (from 0 until 9) the next and such a technique the been used word.

Sweetened,

Defined: oral cavity is dominantly perceived, and approximately recognition of sweetened, ..

Evaluation manner: synchro of has chewable product until eliminated. Evaluation a strength of sweetened,.

Recording: 0 = month not go, 9 = very sew on.

it buys taste

Defined: oral cavity is dominantly perceived, and. approximately recognition it buys taste.

Evaluation manner: synchro of has chewable product until eliminated. Evaluation a it buys taste strength.

Recording:. hardly 0 =, 9 = quite rart.

Method for preparing sample

A cold melon-. then being washed with water.

-A is cut in a direction a longitudinal melon.

-Fruit body and seeds and isolated.

Each as a result of anti- melon -3 ., the cutter cuts the portion (cutting longitudinal direction).

-3 portion ending of removing the.

-Each portion 6 for as a result of anti- melon cutting into both portion.

Product provided

-random number ranked code of presentation order sample to eliminate the influence of provides adjusted depending on the planned provided.

-Products are provides one.

Table 14 and a 15 whereon a plurality of fruit to a embodiment presented the analyzing result is functional Conference 2 is formed inside the tie.. In table 14, plants are 3 4 the in the protective the beginning of the monththe beginning of the monthopening. 7 the implant as crop creeper paper the beginning of the month to the harvesting fruit. Table in 14A, fruit 10 °C 7 to 12 in then of long-term storage in the 20 °C in man stored up during 2. Table 10 °C 14B fruit in then-time storage 8 to 4 in 2 20 °C man stored up in between.

Table 15 8 plant in the 8 month the beginning of the month in the plastic house was implantation as crops to pile. Fruit was harvested in the beginning of the month 11. Fruit 5 °C in in 20 °C then of long-term storage in the 4 to 8 man stored up during 3.

Presented table numbers of fruit to functional analysis a entitlements are name. 12 embodiment the taste taster were examined each fruit. Each taste per taste and acid for automotives for x 12 average number of fruit digits (taster 12) exhibits and. Table 14 and 15 of fruit the inspected in addition the organic acid alcohols, and sugars pH measurement value obtained by a content of wet liquid to flow down also presented.

In the embodiment 13: molecular markers analysis

Young leaves (wood Young zero 15) in a DNA extracted. Leaves [...]dell by freeze-drying method of a DNA according to (Dellaporta 1983) extracted.

PCR circulation conditions as follows: 15 in 94 °C modified seconds, then 15 seconds annealing in 54 °C, 72 °C and extending 40 times in circulation of embodiment 30 seconds. 94 °C first DNA sample 2 in the modified minutes, 2 minutes after PCR in 72 °C reacted extending.

PCR mixture contains 1.65 millimeter MgCl₂, each [...] 60 millimeter, 1x Taq buffer, 0.2 unit Taq polymerase, DNA mold 20ng to 15 400 nanometer or fluorescent primer each and non-radiolabeled each primer containing 200 nanometer of wet liquid to flow down. Fluorescent primer 6-FAM, he labeling to HEX or NED. Fluorescent PCR products is separated into analyzer sequence capillary ABI3700 each size of [...] bio [...]fourth scantype it ladles, xenotime and it was determined that process recursive virtual machine instructions using software analysis fragments.

Non PCR fitted with such a cooling plate system product 3% agarose gel (Resophor, Eurobio) separated the under electrophoretic in the 400V in. Iridium bromide for dying the gel the ethinyl. 3 was use of a marker of: CMAT 141 (Danin-Poleg et al. (2001) Theor. Appl. Genet. 102 : 61-72 and Danin-Poleg et al. (2002) Euphytica 125 : 373-384), and NE0585 and NE1746. For of this marker were as follows. primer.

In the embodiment 14: consigned objects analysis

Low pH consigned objects melon a plurality of containing traits for the analyzed using said marker. The size of the fragments amplified hereinafter table 16 to he got down the main. Recording size (bp) similar primer pairs, detected by using a recombinant plant expression vector containing of different sizes which was not absolute relative thereof in the following. Therefore, actual fragments amplified (accurate) size (e.g. measured in millilitres sequence analysis) and the to allow the depressed parts to be. may vary slightly (+ /-1bp).

In the embodiment 15: fruit these plants fruit of change of the features when the smart memory module is present

Said table 15 presented to the plants was in the channel to support a plant such as 2d gratings. The harvesting fruit (HDT) and moisture (Pacific Daylight Time) the difference between point mature various computed through the harvesting according (period). At any particular time in period shrinp fruit per 100g fwt term content of sucrose to 2.0g only memory, a random access memory, and laterally leaf senescence in mature other data such as using symptoms responding to specific periods was deemed 30 never interfere with each other. The results are illustrated in table 17.

Each respective result the n bit parallel data inputted based on a measured value of the fruit, some fruit is damaged, or more change intellectdevelopment and other environmental provided different values may be loaded with.

In the embodiment 16: change of the features fruit after harvesting

A. SP03OF plant experiment (table 6 and 7C reference) in 12 °C the harvesting fruit of main 1, then store or daytime 1 in 20 °C, 12 °C in main 6, then 20 °C 2 man stored up in between. Fruit after storage for measuring characteristics of've got the to and table 18.

B. Table said plant culturing was equal to plant 14. In the harvesting fruit 12 °C main 1, then 20 °C 3 man stored up in between. Fruit after storage for measuring characteristics of've got the to and table 19.