ANTIFUNGAL COMPOSITION AND METHOD FOR PRODUCING POPULUS TOMENTIGLANDULOSA T. B. LEE EXTRACT HAVING EXCELLENT EFFECTS OF SUPPRESSING GROWTH OF RHIZINA UNDULATA

Hereinafter, the present invention more rapidly and to reduce a memory..

As described above by plant pathogenic fungi for the onset of pest illness the crops, weeds stable crops with very large production by the causes number.. Such as method for pathogen room number has an improved culture environment a physical method, using number sterilization using microbes and chemical method such as electrochemical method. However physical room number loaded which cannot the windmill, the chemical room number, a chemical by agricultural chemicals an environmental pollution is door number and a pesticide number number number room the regeneration pathogen resistance is extended to be rotated. Is of replacement scheme by microorganisms antagonists as number constitution: liquid silicon is recent biological room actively. filter of reduced hole size, which the processing.

In particular, ridge my root the bottle rotting room in number in the case of some enterocyte-contains about the damage to do, toxicity and environment number door consisting of use due to objects' traces to which one number. The ridge my root the bottle rotting mushroom jellyfish ground door is engaged causing the room number (Rhizina undulata) a method number about and special devices, thin film transistors are electrically connected to a room wound on a clean roll and a number is situations development of method.

The extract obtained by extracting several Chinese wooden in the present invention refers to silver in silver step of bath number extract heat tree including ridge and for circuit controlling isolation gates of growth my root the germ rotting number of extract in silver effect; a process for manufacturing the manufacturing method of the above-mentioned door number by number public box been made to solve them by providing the fitting corresponding advertisement based on the shown list, through plant by the accommodation of influencing or change or metabolic function nucleotide sequence of active normal physiologically causes pathogens that part is not on-, (Rhizinaundulata) my root the germ rotting ridge in particular to formula 1. antibacterial effect is.

In the present invention refers to silver in silver core yarn, extract obtained by extracting several Chinese wooden wood heat extract my root the germ rotting ridge steps for bath number and for circuit controlling isolation gates of growth in number effect; a process for manufacturing the silver can be under trillion number extract.

Said silver core yarn, in the extract of influencing or intrusion plants with a nucleotide sequence of active change or metabolic function physiologically non-normal (- mongolicaequercus) elevates a pathogens that antibacterial effect to as a material having a, preferably ridge my root the germ rotting can be number and for circuit controlling isolation gates of growth.

Said ridge my root the germ rottingmy root the bottle rotting which may have caused the ridge may include all pathogens, preferably mushroom jellyfish ground door is engaged (Rhizinaundulata) may include a.

On the other hand, wood in silver of the present invention (using lumber: Populustomentiglandulosa) has a perspective view tree (P. Davidiana) and a silver hundred amount (P. Alba) between is hybrid natural elements derived from the irradiated gas may. Biological classification (Angiospermae) utilises carbonised to (Plantae) pizza plant doorplant river dicotyl [...] (Dicotyledoneae) salix neck (Salicales) then (Salicaceae) as a plant, wherein the plant is intrusion of the to, is silver-colored is bark army lower their leaves in particular the beautiful is S430 of winter after planting induction or planting floppy disc driver, can be used to planting buffer.

While, when silver the present strabismus: other tree to times called tree, specifically, wood perspective view tree the present strabismus (P. Davidiana) and a silver hundred amount (P. Alba) between wood-in silver core yarn, as hybrid artificial elements derived from the irradiated gas may the same using lumber use an. Thus, when silver of the present invention a hybrid artificial tree can be including general outline wooden the present strabismus.

Surfaces of a structure of wood, in which on the inside of the outwardly from S430 oratorical talent part heartwood department and including Thunberg, layer and can be constructed to bark.

In other words, outside of the of layer about wood-defining layer may be formed is bark, inside the layer of may be formed is Thunberg, when silver used in the present invention when silver tree may include wood-defining layer, preferably includes formation layers are wood in silver core yarn,, silver core yarn, in at least one of Thunberg and cortex of tree may further include any.

Said silver tree in a general solvent extraction may be product economically since the reaction is carried applied, using distilled water as a solvent a heat and to apply heat to the wood in silver core yarn, through extraction can be under trillion number extract heat.

Preferably said silver core yarn, in 90 - 130 °C tree, preferably at a temperature of 100 - 125 °C distilled water in silver core yarn, by extracting with a solvent extract heat tree can be under trillion number, said range at this time, the substrate, and for circuit controlling isolation gates of growth my root the germ rotting ridge number extracts whereby is obtained a can be excellent.

As such, when silver in silver core yarn, through extraction sleeve tree extract of a plant mixture is provided number my root the germ rotting a ridge reason for bath and for circuit controlling isolation gates of growth to obtain extracts number is to produce a rounding off on, reduces blood cholesterol, platelet if non-methanol, it burns and it comes a solvent alcohol such as in silver growth my root the germ rotting ridge if obtained extract and for circuit controlling isolation gates of number extracts. may or may not be obtained.

On the other hand, said wood when silver in the extraction sleeve, the extraction time - 100 ingredient 10 ingredient, preferably 10 - 40 ingredient, extraction count burn 1 - 4, preferably can be burn 2 - 3.

While, the present invention refers to silver in wooden alcohol by extracting with alcohol solvent and for obtaining the extract said alcohol extract in silver core yarn, extract obtained by extracting several Chinese wood heat extract through step of bath number in silver can be under trillion number extract.

Specifically, first silver core yarn, when wooden alcohol by extracting with alcohol solvent. for obtaining the extract. Said acid and alcohol areC₁ - C₄alcohol one selected from the group consisting of the height alcohol, preferably ethanol, use can be made of, .

Alcohol solvent of the present invention to initiator including alcohol or alcohol and 100% in a solvent which, mixed with the refrigerant, a other can be. In this case other as a solvent preferably water, ethyl acetate, chloroform and hexane fixing which, alcohol solvent in the event of the use of solvent water to 50 - 95 weight %, preferably 60 - 80 weight % having concentrations of an alcohol aqueous solution use the extraction efficiency of 30MHz - can be, which are highly advantageous for.

Silver core yarn, when woodenC₁ - C₄in extraction of alcohol solvent, 18 - 70 °C temperature extraction, the extraction time 20 - 25 °C preferably 20 - 72 time, preferably 22 - 26 time, extracting count burn 1 - 4, preferably can be burn 2 - 3.

Next, extract obtained by extracting several Chinese silver core yarn, in said alcohol extract wood heat extract. under trillion number.

At this time, the pressure-reducing concentrated said alcohol extract, condensing with decompressing alcohol extract in silver core yarn, extract obtained by extracting several Chinese wood heat extract can be under trillion number.

As such, when silver alcohol by extracting with alcohol solvent wooden obtaining extract, extract obtained by extracting several Chinese obtained alcohol extract in silver prepared by the method number good ridge: a hot-water extract of wood and for circuit controlling isolation gates of growth my root the germ rotting can be using the number.

Further, the manufacturing method of the present invention extract in silver of said hot-water extract to chloroform in the wood in silver( Chloroform: CHCl₃)of the fraction extraction solvent including further bath number fractions may include.

The silver is at the ridge of the extract in and for circuit controlling isolation gates of growth my root the germ rotting number as steps for time to further increase its efficiency, said fractions THon my root the germ rotting ridge than hot-water extract wood in silver and for circuit controlling isolation gates of growth can be superior to effect number.

Specifically, in the present invention refers to silver in silver core yarn, extract obtained by extracting several Chinese wooden number extract heat wood and with a bath hot-water extract to chloroform in the wood in said silver( Chloroform: CHCl₃)for including extraction solvent fractions the fraction number bath for step number extract in silver can be under trillion.

In addition, when silver core yarn, the present invention refers to alcohol by extracting with alcohol solvent wooden obtain extract, extract obtained by extracting several Chinese silver core yarn, in said alcohol extract number extract heat wood and bath hot-water extract to chloroform in the wood in said silver( Chloroform: CHCl₃)for including extraction solvent fractions the fraction number bath for step number extract in silver can be under trillion.

As such, hot-water extract wood in silver using chloroform solvent extracted the reason for this is that the chloroform in silver core yarn, a solvent containing the extracts heat tree my root the germ rotting ridge, a fraction thereof and for circuit controlling isolation gates of growth must number extracts is since it can be is obtained. In other words, in addition to solvent chloroform [...] (hexane) or ethyl acetate (ethyl acetate) in silver core yarn, a solvent, a fraction containing the extracts heat tree if and for circuit controlling isolation gates of growth my root the germ rotting ridge number extracts. may or may not be obtained.

While, when silver core yarn, said to pull down and rebuild aforementioned extract my root the germ rotting the ridge which number and for circuit controlling isolation gates of growth, growth my root the germ rotting ridge preferably and for circuit controlling isolation gates of number can be 90% or more, more preferably 100% can be number and for circuit controlling isolation gates of up.

Further, the present invention refers to silver in extract as active ingredient and for circuit controlling isolation gates of growth my root the germ rotting ridge including antibacterial effect; a process for manufacturing the number number. under public affairs number composition.

At this time, in the extract of the aforementioned items to said silver in silver number in manufacturing method of extract can be extract prepared by the method.

Specifically, when the silver in said silver tree extract extracted sleeve may be hot-water extract wood in silver, silver core yarn, when wooden alcohol extraction of alcohol solvent extracted the heat extract can be hot-water extract wood in silver.

Said acid and alcohol areC₁ - C₄alcohol one selected from the group consisting of the height alcohol, preferably ethanol, use can be made of, .

In addition, the extract when said silver in silver alcohol extraction of alcohol solvent wooden the heat extract extracted silver for to chloroform in the hot-water extract wood in that the fractions extraction solvent including CH3, followed by immersed alcohol wooden in silver, in silver extracted heat for to chloroform in the hot-water extract tree including extraction solvent. can be that the fractions.

Said acid and alcohol areC₁ - C₄alcohol one selected from the group consisting of the height alcohol, preferably ethanol, use can be made of, .

In addition, when silver composition of the present invention antimicrobial number in silver tree may include wood-defining layer, preferably includes formation layers are wood in silver, silver core yarn, in at least one of Thunberg and cortex of tree may further include any.

In addition, ridge composition number antibacterial of the present invention and for circuit controlling isolation gates of growth my root the germ rotting the number, said ridge my root the germ rotting mushroom jellyfish ground door is engaged (Rhizina undulata) may include a.

In addition, ridge composition of the present invention and for circuit controlling isolation gates of growth my root the germ rotting antibacterial number which number, growth my root the germ rotting ridge preferably and for circuit controlling isolation gates of number can be 90% or more, more preferably 100% can be number and for circuit controlling isolation gates of up.

While, the present invention refers to the above - mentioned antibacterial activity against antimicrobial number number a number which under public affairs including composition, said antibacterial activity against the ridge my root the germ rotting number can be number and for circuit controlling isolation gates of growth.

In the embodiment of the present invention through the account but for more specifically, in the embodiment a of the present invention is not the only number range, for assisting the understanding of the present invention should be interpreted to will.

In the embodiment

Preparing e.g. 1: silver core yarn, in cortex of wood, bark including-defining layer, including-defining layer number Thunberg and Thunberg bath

Institute with forest National of safety valve in silver of distal gene, gluconic acid, tannic acid, S430 after as blocks, wood, bark, bark including-defining layer, including-defining layer separated the to Thunberg and Thunberg.

Preparing e.g. 2: culture method

Ridge parts of a wooden pine infected my root in the germ rottingmy root the germ rotting ridge from (Rhizinaundulata) corresponding advertisement based on the shown separation purified, isolated pathogens include 25 °C, good in cancer conditions.

In the embodiment 1

After the 1 e.g. ready crushed to wood in silver of S430 including formation layers are isolated from possible with conventional distilled water to 500 ml 60g bark, into 3 20 121 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number of hot-water extract was under trillion.

In the embodiment 2

In the embodiment 1 in the same method was under trillion number extract heat wood in silver.

Just, preparing e.g. wood in silver of 1 isolated from S430 instead Zygorhynchus moelleri pf - 425 isolate, including-defining layer 1 preparing e.g. wood in silver of isolated from S430 the first voice portion out of an Thunberg including-defining layer.

In the embodiment 3

In the embodiment 1 in the same method was under trillion number extract heat wood in silver.

Just, preparing e.g. wood in silver of 1 isolated from S430 instead Zygorhynchus moelleri pf - 425 isolate, including-defining layer 1 e.g. preparing isolated from S430 wood in silver of 10g Zygorhynchus moelleri pf - 425 isolate, by cylinder to have.

In the embodiment 4

In the embodiment 1 in the same method was under trillion number extract heat wood in silver.

Just, preparing e.g. wood in silver of 1 isolated from S430 instead Zygorhynchus moelleri pf - 425 isolate, including-defining layer 1 preparing e.g. wood in silver of 50g Thunberg isolated from S430 by cylinder to have.

Compared example 1

After the 1 e.g. ready crushed to wood in silver of S430 including formation layers are isolated from possible with conventional 500 ml ethanol to 60g bark, room temperature 3 hours a 48 a 20 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number was under trillion ethanol extract.

Experiment example 1

Antimicrobial activity assay used for culture media a ridge my root the germ rotting culture medium diameter 9 cm PDA (potato agar medium) medium under trillion instead distilled when a number in number in the embodiment 1 - 4 by the addition of extract prepared by the method number is stabled under trillion, compared e.g. 1 in the case of a PDA (potato agar medium) medium after under trillion number, media are immediately before allows for quicker by the addition of ethanol extract was under trillion number. Each extract added medium 2 e.g. ready in a cork bolus my root the germ rotting ridge in (5 mm) and specific sized centrally locating of an overlay medium, within a 25 °C and, cancer conditions in a culture is 5 then, grown of mushrooms diameter in the range of from measuring embodiment the antibacterial activity assays.

Spawn number and for circuit controlling isolation gates of growth rate (%) 1 expressions to operate close to the loop frequency is measured by use of the, described to result to the computer of the table 1.

[Expressions 1]

The diameter 9 cm in 1 said expressions 9 cm size PDA. meant the amount growth maximum in culture media.

Table 1 e.g. inflammatory disease and 1 of wood in silver including formation layers are isolated from S430 using bark extract heat wood in silver under trillion 1 e.g. and preparation in the embodiment 1 a number of wood in silver including formation layers are isolated from S430 using Thunberg number extract heat wood in silver under trillion the 90% or more in the embodiment 2 a, preferably 95% or more ridge and for circuit controlling isolation gates of growth my root the germ rotting can be identifying 80 M. a number.

In addition, preparing e.g. wood in silver of 1 isolated from S430 in silver core yarn, using bark extract heat tree under trillion a number in the embodiment 3, 1 e.g. preparing isolated from S430 wood in silver of using Thunberg number extract heat wood in silver under trillion 1 a in the embodiment 4 and preparation e.g. wood in silver of isolated from S430 including-defining layer for comparison extraction into ethanol Zygorhynchus moelleri pf - 425 isolate, 1 e.g. number and for circuit controlling isolation gates of growth my root the germ rotting the ridge does not exhibit could confirm it.

In the embodiment 5

After the 1 e.g. ready crushed to wood in silver of S430 including formation layers are isolated from possible with conventional 500 ml ethanol to 60g bark, room temperature 3 hours a 48 a 20 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number was under trillion ethanol extract.

500 ml distilled possible with conventional leaf extract, chitin, chitosan and said ethanol, into 3 20 121 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number of hot-water extract was under trillion.

In the embodiment 6

In the embodiment 5 in the same method was under trillion number extract heat wood in silver.

Just, preparing e.g. wood in silver of 1 isolated from S430 instead Zygorhynchus moelleri pf - 425 isolate, including-defining layer 1 preparing e.g. wood in silver of isolated from S430 the first voice portion out of an Thunberg including-defining layer.

In the embodiment 7

In the embodiment 5 in the same method was under trillion number extract heat wood in silver.

Just, preparing e.g. wood in silver of 1 isolated from S430 instead Zygorhynchus moelleri pf - 425 isolate, including-defining layer 1 e.g. preparing isolated from S430 wood in silver of 10g Zygorhynchus moelleri pf - 425 isolate, by cylinder to have.

In the embodiment 8

In the embodiment 5 in the same method was under trillion number extract heat wood in silver.

Just, preparing e.g. wood in silver of 1 isolated from S430 instead Zygorhynchus moelleri pf - 425 isolate, including-defining layer 1 preparing e.g. wood in silver of 50g Thunberg isolated from S430 by cylinder to have.

Experiment example 2

Antimicrobial activity assay used for culture media a ridge my root the germ rotting culture medium diameter 9 cm PDA (potato agar medium) medium under trillion instead distilled when a number in number in the embodiment 5 - 8 was under trillion number by the addition of extract prepared by the method. Each extract added medium 2 e.g. ready in a cork bolus my root the germ rotting ridge in (5 mm) and specific sized centrally locating of an overlay medium, within a 25 °C and, cancer conditions in a culture is 5 then, grown of mushrooms diameter in the range of from measuring embodiment the antibacterial activity assays.

Spawn number and for circuit controlling isolation gates of growth rate (%) 1 expressions to operate close to the loop frequency is measured by use of the, described to result to the computer of the table 2.

[Expressions 1]

The diameter 9 cm in 1 said expressions 9 cm size PDA. meant the amount growth maximum in culture media.

Table 1 e.g. inflammatory disease and a 2 wood in silver of isolated from S430 including-defining layer and refitted again extraction of solvent into ethanol bark extract obtained by extracting several Chinese number the like heat wood in silver prepared by the method in the embodiment 5 and preparation e.g. wood in silver of 1 isolated from S430 including-defining layer and refitted again extraction of solvent into ethanol Thunberg extract obtained by extracting several Chinese wood in silver prepared by the method number in the embodiment 6 the like heat the 90% or more, preferably 95% or more ridge and for circuit controlling isolation gates of growth my root the germ rotting can be identifying 80 M. a number.

In other words, in the embodiment 5 and in number in the embodiment 6 in the embodiment 1: a hot-water extract of wood in silver prepared by the method in the embodiment 2 and similar hot-water extract wood in silver prepared by the method number in a ridge with and for circuit controlling isolation gates of growth my root the germ rotting number was capable of verifying 80 M. rate.

In the embodiment 9

9 - 1. Number bath hot-water extract wood in silver core yarn,

After the 1 e.g. ready crushed to wood in silver of S430 including formation layers are isolated from possible with conventional 500 ml ethanol to 60g bark, room temperature 3 hours a 48 a 20 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number was under trillion ethanol extract.

500 ml distilled possible with conventional leaf extract, chitin, chitosan and said ethanol, into 3 20 121 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number of hot-water extract was under trillion.

9 - 2. Number of hot-water extract wood in silver bath

Wood in silver prepared by the method in the embodiment 9 - 1 number in hot-water extract to chloroform in the organic solvent and solvent fraction, the n bit parallel data inputted extraction iterations 3 same, corresponding advertisement based on the shown list filtered filter paper, same fractions wood in silver core yarn, concentrated under the reduced pressure was under trillion number.

In the embodiment 10

In the embodiment 9 in silver core yarn, in the same method was under trillion number fractions tree.

Just, preparing e.g. wood in silver of 1 isolated from S430 instead Zygorhynchus moelleri pf - 425 isolate, including-defining layer 1 preparing e.g. wood in silver of isolated from S430 the first voice portion out of an Thunberg including-defining layer.

In the embodiment 11

11 - 1. Number bath hot-water extract wood in silver core yarn,

After the 1 e.g. ready crushed to wood in silver of S430 including formation layers are isolated from possible with conventional 500 ml ethanol to 60g bark, room temperature 3 hours a 48 a 20 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number was under trillion ethanol extract.

500 ml distilled possible with conventional leaf extract, chitin, chitosan and said ethanol, into 3 20 121 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number of hot-water extract was under trillion.

11 - 2. Number of hot-water extract wood in silver bath

In the embodiment 11 - 1 number [...] extract heat wood in silver prepared by the method in (Hexane) with an organic solvent and solvent fraction, the n bit parallel data inputted extraction iterations 3 same, corresponding advertisement based on the shown list filtered filter paper, same fractions wood in silver core yarn, concentrated under the reduced pressure was under trillion number.

In the embodiment 12

12 - 1. Number bath hot-water extract wood in silver core yarn,

After the 1 e.g. ready crushed to wood in silver of S430 including formation layers are isolated from possible with conventional 500 ml ethanol to 60g bark, room temperature 3 hours a 48 a 20 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number was under trillion ethanol extract.

500 ml distilled possible with conventional leaf extract, chitin, chitosan and said ethanol, into 3 20 121 °C in the n bit parallel data inputted extraction iterations, corresponding advertisement based on the shown list filtered filter paper, wood in silver core yarn, concentrated under the reduced pressure same number of hot-water extract was under trillion.

12 - 2. Number of hot-water extract wood in silver bath

In the embodiment 12 - 1 in ethyl acetate extract heat wood in silver prepared by the method number (Ethyl acetate) with an organic solvent and solvent fraction, the n bit parallel data inputted extraction iterations 3 same, corresponding advertisement based on the shown list filtered filter paper, same fractions wood in silver core yarn, concentrated under the reduced pressure was under trillion number.

Experiment example 3

Antimicrobial activity assay used for culture media a ridge my root the germ rotting culture medium diameter 9 cm PDA (potato agar medium) medium under trillion number in number in the embodiment 9 - 12 a instead distilled when the number was under trillion adding the fraction prepared by the method. Respective fractions ready in a medium the addition of water in 2 e.g. cork bolus my root the germ rotting ridge (5 mm) and specific sized centrally locating of an overlay medium, within a 25 °C and, cancer conditions in a culture is 5 then, grown of mushrooms diameter in the range of from measuring embodiment the antibacterial activity assays.

Spawn number and for circuit controlling isolation gates of growth rate (%) 1 expressions to operate close to the loop frequency is measured by use of the, described to result to the computer of the table 3.

[Expressions 1]

The diameter 9 cm in 1 said expressions 9 cm size PDA. meant the amount growth maximum in culture media.

Table 3 in the embodiment 9 fractions of chloroform, such as in the embodiment 10 and the number and for circuit controlling isolation gates of growth my root the germ rotting is at the ridge of the 100% for thewater in a manner that renders the capable of verifying 80 M., the silver core yarn, in wood heat the like in the embodiment 1, 2, 5, 6 and for circuit controlling isolation gates of growth my root the germ rotting ridge than number is excellent in can be confirm that the user.

In addition, in the embodiment 11 acid and ethyl acetate (Hexane) fractions of [...] (Ethyl acetate) fractions of in the embodiment 12 and for circuit controlling isolation gates of growth my root the germ rotting the ridge does not exhibit number could confirm it.