NOVEL PHAEODACTYLUM TRICORNUTUM STRAIN AND METHOD FOR PRODUCING BIODIESEL USING SAME
The present invention refers to novel bio-diesel of microalgae strains and manufacturing method relates to search, and more particularly to novel [...] tree [...] MBEyh07L preferably the novel strain manufacturing method of bio-diesel are disclosed. While petroleum reserves is reduced, an increase in population from an increase in energy consumption is produced etc. alternative energy exploitation of resources. Number door environment such as global warming and eco-friendly development of alternative energy not available for substantially tutors in perfumery. Alternative energy such as solar, wind, geothermal, tidal power and intensive etc. to one or stability of the method. However, such as electric furnace produced by using said physical earth ship, airplane and to replace currently used for transporting energy source and using all infrastructure in order to free the spirit energy for storing liquids. , the condition is said alternative energy resource etc. as to penetrate through the biodiesel. The biodiesel is from biomass Transesterification process created through FAME (Fatty Acid Methyl Ester) saying the other. Soybean oil or palm oil such as vegetable oil or connect, birds and the like such as a beef animal fats and biodiesel production feedstock is disclosed disclosed. In particular, the microalgae SO4 ions are used more rapidly from a biodiesel feedstock that was communicated to the lipid content such as palm oil beans etc. to penetrate through land plant alternative material. The configuration of the of fossil fuel plants would land microalgae biodiesel fatty acid are similar fatty acid is in many cases disclosed. A cetane, viscosity, density of existing infrastructure such as receiving of similar fossil fuel internal combustion engine using integral CPU etc. simultaneously. Main the fatty acid (palmitic acid) constituting biodiesel C16:0, C18:0 (stearic acid), C18:1 (oleic acid), C18:2 (linoleic acid), a variety of fatty acid C18:3 (linolenic acid) to be disclosed. The safety and cold flow it is possible to determine a quality biodiesel of compositional fatty acid oxidation associating tumors with each other. In particular, double bonds according to the determined quality of the biodiesel, polyunsaturated fatty acids (PUFAs) but the content of middle distillate compositions to inhibit oxidation stability improving substrate. On the contrary the content of saturated fatty acids (SFAs) is to improve oxidation stability but is equal to middle distillate compositions. A raw material for production of biodiesel displayed soybean, palm oil and fatty acid composition PUFAs SFAs etc. on microalgae is a substantial amount of charge. In the case of soybean oil PUFAs are 53 during the entire fatty acid content. 2% oxide safety signal is a negative polarity substrate. While, in the case of palm oil SFAs entire content of 42. 8% which is the lower of the first middle distillate compositions, determining middle distillate compositions is under or over when you -4 °C Cold Flow Pulgging Point (CFPP, filter blocking point). Wherein -42 °C against the CFPP of fossil fuel is low numerical are disclosed. Door number forward more middle distillate compositions to improve current oxidative stability for progressing disclosed. Lower oxidation stability is compared with production and storage, and oxidation occurs even during use and can be used in an internal combustion engine are also affect. Butylated hydroxyanisole (BHA) to supplement the same presently, Pyrogallol (PrG) is an intermediate used butylydroquinone (TBHQ), bytylated hydroxytoluene BHT) and alpha-tocopherol substances such as stable to oxidation number been studies. At or material are improved oxidation stability results. However biodiesel production number is large in diameter and number are also conductive frost adverse effects on other. Low biodiesel middle distillate compositions when used at a low temperature, the engine filters diesel crystallization is performed and a starter film formed in the cover substrate. In order to improve the most generally used the same amount of kerosene is biodiesel mixing method are disclosed. The method is low in using the CFPP -20 °C or more middle distillate compositions can be further improve. The mirror number method but not available from the supply of kerosene as well as good frost does not timer does not work disclosed. According to attempts to part through suitable the biodiesel is single unsaturated fatty acid (monounsaturated fatty acids, MUFAs) can be made equal to that. The, oxidation stability and high pressure liquid coolant there is provided a middle distillate compositions simultaneously good biodiesel MUFAs number order to a process for producing species of formula where name is disclosed. The present invention refers to said door such as number points to solve various door number points provided, oxidation stability and cold flow while at the same time the alkali water and biodiesel is MUFAs fatty acid can improve mass-producing strains intended for the novel [...] tree [...]under public affairs number. Example number is however and generally, the range of the present invention is defined in which are not correct. Selectively implanted into the of the present invention, tree [...] consignee number KCTC 18366P in novel [...] MBEyh07L strain ( The rDNA sequence number 1 having the nucleic acid sequence of said strain. In the embodiment according to publicly known strains of the present invention novel [...] tree [...] MBEyh07L [...] growth rate of one homogeneous strains and compared, content of the hinge MUFAs, even field duplication steel disclosed. As described above, would exert a substantial amount on microalgae biodiesel fatty acid composition SFAs PUFAs is because the inside of the first cold flow-oxidation stability but, in the embodiment according to the voltage signal of the present invention novel [...] strains such a door number one tree [...] MBEyh07L, highly available industrial 0.5 or 1. According to another aspect of the present invention, said novel [...] tree [...] MBEyh07L strain as an active ingredient a composition for producing biodiesel encoded ball number. Said composition for inducing strain in said novel [...] tree [...] MBEyh07L lipid ester conversion catalyst for, lower alcohols and fatty acid ester separated positive number can be included for non-polar organic solvents. The solid catalyst or said catalyst liquid catalyst may be, the total amount of said liquid catalyst, acetyl chloride or sulfuric acid/methanol mixed solution may be, said solid catalyst and acid be a processed bio charcoal. Said lower alcohols may be methanol or ethanol, said non-polar organic solvents pentane, cyclopentane, hexane, cyclohexane, benzene, toluene, 1, 4 - dioxane, chloroform or diethyl ether be a. According to another aspect of the present invention, said [...] tree [...] MBEyh07L strain ( Said method in production of biodiesel, fatty acid ester formed after said converting further comprises the step number positive biodiesel bio-diesel from biodiesel can be positive number. The present invention victims of the marine culture device in order to develop adaptive excellent holding field in a marine culture strains also collects the seawater in the beach yonghung signed his separating microalgae hydrophobin. One of excellent plasma treatment process of selecting species switchable fatty acid methyl ester high lipid content identifying result tree [...][...] ( The present invention according to [...] tree [...] ( Figure 1 according to one embodiment of the invention is a novel [...] tree [...] strain The present invention through a detailed hereinafter in the embodiment as follows. In the embodiment of the present invention to but the present invention is exemplified ephemeral contents in the embodiment is limited to a different but can be implemented in various forms. In the embodiment 1: on derivatives In the neighborhood of the microalgae culture collection of thermal power stations also contain a compensation yonghung marine sea water separating the matter field. Artificial seawater (MBL) to f/2 3x through the sterilising been seeded onto a medium. The middle of the growth of the microorganisms by using artificial sea water have natural seawater back number, f/2 3 times-free medium amount reduces generation of microalgae by separation time can shorten his. The composition of the composition of table 1 to f/2 MBL such as disclosed. Said MBL f/2 agar (agar) inoculation of cells 1. 5% to 20 °C 100 ± 5 μEm flat medium in human herpesvirus-2 s-1 As to sequentially illuminate the optical housing of him as daytime while 3. Quickly formed in guide feet microalgae was caused by this linear herpesvirus (streaking) platinum flat medium. The following experiments were repeated 5 [...] microalgae pure guide to a fuel tank within. After this the periodically are excellently his embodiment. In the embodiment 2: identification of strains In separating in the embodiment 1, the microalgae culture for identifying said morphological observation and 18s rDNA sequence analysis in strains through microscope relative to base his database. (Genomic) DNA genome of strains for separating, separate from the 18s rRNA gene PCR positive number the matter. PCR primer pairs used for information about a table 2 of 18s rDNA gene described disclosed. PCR positive number when the sequence homology is a recombinant nucleic acid sequence analysis program (Blast search and cluster W) multiple bio-sequence listing by comparing the affinity analysis of abortion. As a result 967 bp PCR product of the nucleic acid sequence of 18S rDNA fragments isolated from strain (also 2) (sequence number 1) identifying said first call using NCBI BLAST of homologous nucleic acid sequences where the analysis results, In the embodiment 3: [...] sp [...] tree. MBEyh07L-growth In the embodiment of the present invention separated from and identifying the one The stopper to him as bottle and 0.50 to function cap 250 ml silica. F/2 using 100 ml medium have, biological weight 0 initial inoculation. 1 g/L concentration which reduces, in 100 μEm 20 °C-2 s-1 Of brightness to contrast period (12:12) because to him as being about 150 rpm. Microscope (Nikon, Digital Sight, dS provided Fi1, Japan) using, Hemacytometer were measured using the cell. 10 concentration cells is measured, even [...] tree [...] sp final cellular concentration. MBEyh07L 2 strains. 8 × 107 By grow them to cell/mL has been confirmed. [...] sp [...] tree. MBEyh07L 1 strains. 0 × 106 In cell/mL 1. 0 × 107 Identifying which grow them to cell/mL added to 4 were. In the case of a homogeneous strain 1 reported conventional publicly known. 0 × 106 In cell/mL 6. 2 × 106 While confirming to grow them to cell/mL added to 4, novel [...] tree [...] sp. MBEyh07L the 1. 0 × 106 In cell/mL 1. 0 × 107 4 cell/mL to grow them to drive point in by taking into account, of the present invention novel [...] tree [...] sp. MBEyh07L strain is strain were to 1:1 by weight than said publicly known [...] tree [...] plasma treatment process. In addition, in the case of culture 5 6 and 6 coming true identity giga[...][...] tree [...] strain said publicly known controlled growth. 3 × 106 Therefore rather include reduced while after vertex a cell/mL, novel [...] sp [...] tree of the present invention. MBEyh07L strain of media without replacement, have uncovered to culture growth away at 9, maximum concentration 2. 8 × 107 Cell/mL has been. This when considered, novel [...] sp [...] tree of the present invention. MBEyh07L [...] tree [...] strains said publicly known strains compared throughput biomass 4. 6 highly times can be know. In addition in the case of a single double bond fatty acid, dried cells of the present invention as viewed with reference to novel [...] tree [...] sp. MBEyh07L bottom 3 or more times higher strains of contrast can be door frame. In the embodiment 4: [...] sp [...] tree. MBEyh07L bath using number of biodiesel In the ferroelectric layer obtained in the embodiment 2 by using a recovered microalgae biodiesel has been generated. Acetyl chloride (acetyl chloride) lyophilizing cells recovered after methanol and hexane to ester conversion on inducing methyl S (fatty acid methyl ester, hereinafter, referred to as' FAME ' by) fatty acid through a gas chromatography analysis of content for sharing his. As a result fatty acid composition C16:0 19. 9%, C16:1 38. 9%, C20:5 9% fatty acid component is 3 to know capable of major components. This, said publicly known as a prior art [...][...] tree of different fatty acid as a carbon source, in particular, novel strains of the present invention fatty acid during said publicly known about 2 or more times higher compared to the content of MUFAs [...] tree of [...] strains into foods (also 4). Thus, in the embodiment according to one of the present invention novel [...] tree [...] sp. MBEyh07L strains reported to the number of conventional single unsaturated fatty acid content of the bath glass quality biodiesel sufficiently higher than a high strains, high quality raw material tank showing a number useful know capable of biodiesel. The present invention refers to in the embodiment described above with reference to an exemplary is but only to which, if the art therefrom in various deformation and equally to the other person with skill in the art will understand that it is in the embodiment. The technical idea of the present invention defined by appended claim of true technology protection range generated by the will. agency life strain: Korean Institute bio-technology Consignee number: KCTC18366P Consignee date: 20150407 The present invention provides a novel Phaeodactylum tricornutum MBEyh07L strain having excellent environmental adaptability, and to a method for producing biodiesel having improved quality using the strain. COPYRIGHT KIPO 2016 In novel [...] consignee number KCTC 18366P MBEyh07L [...] tree ( According to Claim 1 novel [...] tree [...] MBEyh07L strain as an active ingredient a composition for producing biodiesel. According to Claim 1 strains into the culture medium under culture step culturing novel [...] tree [...] MBEyh07L coating a hydrophobic yarn; and recovering said fatty acid glycerol fatty acid ester present in strain applied strains of catalyst conversion method including a fatty acid ester converting collides production of biodiesel. According to Claim 3, after converting said fatty acid ester formed from bio diesel positive number step is further included number trillion at all five diesel positive biodiesel, biodiesel production method. Compound Usage NaNO3 0. 075 g NaH2 PO4 , H2 O 0. 005 g Trace metal solution (trace metal solution) 1 ml Artificial seawater (MBL) 1 ml Trace metal solution (1 L reference) FeCl3 , 6H2 O 3. 15 g Na2 EDTA,2H2 O 4. 36 g CuSO4 , 5H2 O 0. 0098 g Na2 MoO4 , 2H2 O 0. 0063 g ZnSO4 , 7H2 O 0. 022 g CoCl2 , 6H2 O 0. 01 g MnCl2 , 4H2 O 0. 018 g Artificial seawater (MBL, reference 1 L) NaCl 24. 7 g KCl 0. 66 g MgCl2 , 6H2 O 8. 48 g CaCl2 , 2H2 O 1. 9 g MgSO4 3. 07 g NaHCO3 0. 18 g ITS5 A forward primer 5 'a-gGAAGTAAAAGTCGTAACAAGG-a 3' (seq ID 2) ITS4 Reverse primer 5 'a-tCCTCCGCTTATTGATATGC-a 3' (seq ID 3)
