COSMETIC COMPOSITION INCLUDING NICOTINOYL PEPTIDE AND NATURAL FERMENTED SUBSTANCE

The specification of a term in the present invention used in the ability of a typical possible while selecting but generic terms, this it was found intended or precedent risks caused by the descriptors, such as can be depending on appearance of a new technique. In addition, the applicant is arbitrarily selecting certain terms may, in this case portion comprise corresponding description of the invention based on their meaning will. The terms used in the present invention a simple non-name of a term, the term is defined based on semantics and having throughout the contents of the present invention should.

Not defined differently, scientific or technical terms so that all terms in the present invention thus is provided to the person with skill in the art will generally have the meanings etc. by same. Generally dictionary used for providing language translators such as defined terms have the meanings associated technology must be consistent semantics and having interprets, the application will not become manifest in defining, or overly formal sense interpreted not ideal.

Numerical range comprising said range defined value. The specification given maximum numerical number across all smooth low numerical number written as one of making all the lower numerical number comprises one. The specification given minimum numerical number one higher numerical number of making smooth over all pills after all as is higher numerical number comprises one. The specification given over all numerical number written as one of making the smooth narrower numerical number, all numerical ranges in the range of wider is numerically better will.

Hereinafter, of the present invention in the embodiment described dilate or, in the embodiment to the present invention by holes which are defined by a nontrivial disclosed.

One aspect of the present invention is a peptide of formula 1 may be roh mote nicotinate, galacto vaccine compositions which (Galactomyces) Fermentable matter, and Lactobacillus paracasei (Lactobaillus) Active ingredient fermented including cosmetic composition number [...] substrate.

[Formula 1]

In formula A is composed and said Tyr-a Gly-a Gly-a Phe peptide, one or more said L is any amino acid.

One or more amino acid oligopeptide consisting of said Tyr-a Gly-a Gly-a Phe can be coupled, for example, Tyr-a Gly-a Gly-a Phe-a Met, Tyr-a Gly-a Gly-a Phe-a Leu, Tyr-a Gly-a Gly-a Phe-a Leu-a Arg-a Lys-a Tyr-a Pro, Tyr-a Gly-a Gly-a Phe-a Leu-a Arg-a Lys-a Tyr-a Pro-a Lys, Tyr-a Gly-a Gly-a Phe-a Leu-a Gly-a His-a Lys, Tyr-a Gly-a Gly-a Phe-a Leu-a Lys-a Thr-a Thr-a Lys-a Ser, Tyr-a Gly-a Gly-a Phe-a Leu-a Glu-a Glu-a Met a-Gln-a Arg provided Arg, Tyr-a Gly-a Gly-a Phe-a Leu-a Arg provided Arg a-Ile-a Arg-a Pro-a Lys-a Leu-a Lys, Tyr-a Gly-a Gly-a Phe-a Met-a Thr-a Ser-a Glu-a Lys-a Ser-a Gln-a Thr-a Pro non-Leu-a Val-a Thr, Tyr-a Gly-a Gly-a Phe-a Met-a Thr-a Ser-a Glu-a Lys-a Ser-a Gln-a Thr-a Pro non-Leu-a Val-a Thr-a Leu or Tyr-a Gly-a Gly-a Phe-a Met-a Thr-a Ser-a Glu-a Lys-a Ser-a Gln-a Thr-a Pro non-Leu-a Val-a Thr non-Leu-a Phe-a Lys-a Asn-a Ala a-Ile-a Ile-a Lys-a Asn-a Ala-a Tyr-a Lys-a Lys-a Gly-a Glu handler and not the limited to.

General peptide said designated rules specifically indicated exception processing unit 3 can be based on character or 1 if there is no character amino acid code. For example, amino acid structure (e.g., Ala, Lys) 3 and center of display character code, "D -" in front of the 3 character code (e.g., D non-Ala, D-a Lys) D - by-like conformational otherwise would have to be L - specifically the post can be conformational is acquired. Said amino acid residue peptide [...] be native or non - natural amino acids.

At this time, said nicotinate roh mote from the second oligo peptides may be peptide derivatives, the number of nicotinic acid and coupling reaction can be high pressure liquid coolant.

Said oligo peptides or proteins in vivo molecular weight quantity anger is then subjected to treatment with a protease, gene recombination and protein expression system using high pressure liquid coolant can be biologically number, peptide synthesizer using chemical synthesis method such as high pressure liquid coolant to number disapproval.

Specifically, the solid phase peptide synthesis (solid phase peptide synthesis: SPPS) NH to said oligo peptides (a) conventional₂ - Protected peptide - resin to yield; (b) obtained NH₂ - - Nicotinic acid by reacting protected peptide resin; and (c) number number bath formed by resin industry can be disclosed. If there is said side chain functional groups constituting said oligo peptides (a) amino acid residues is in said functional groups can be protected amino acid peptide synthesis, (c) protecting group is coupled to said functional groups in special number can be disclosed.

The content of 50 to 10,000 ppm as the weight of said aqueous solution may be peptide derivatives roh mote nicotinate 0. 005 To 1. 5 Implementation being % by weight.

Said skin protection and skin condition improving effect, skin whitening, skin aging and for preventing or ameliorating, skin care and skin of relieving inflammatory responses, employed for improving immune diseases, or improving skin barrier function, skin-irritation, skin cell proliferation and dehydrogenase, antioxidant capability, such as promoting collagen synthesis employed for skin-beneficial effect can be mapped.

Said "active ingredient including" a cosmetic composition for skin effect can be, e.g., wrinkle efficacy associated with collagen synthesis, representative of the extent to which the elasticity enhancer containing an effective amount of antioxidant activity or which means that the can.

Said active ingredient roh mote peptide exhibits said said cosmetic composition may be so skin moisturizing, or improving, steroidal anti-inflammatory skin and having excellent effects that a disclosed. In particular, said cosmetic composition containing said nicotinate roh mote peptide and at least one treatment or may be due to a synergistic effect of various active ingredient effect can be significantly enhanced.

Said fermentation comprises specific natural matter, such as fermented plant extract can be obtained. The enzyme can be a process using said microorganism fermentation organic organic decomposition can be converted into useful products by fermentation said shade is installed. In particular, natural lactic acid bacteria or yeast fermented with increased collagen biosynthesis has been obtained of the present invention reduced toxicity effect employed, so that the collagen corner strip capacity, preventing skin aging, skin wrinkle, improvement can be used to enhance skin elasticity.

Said galacto vaccine compositions which (Galactomyces) And Lactobacillus fermentation (Lactobaillus) Fermentation comprises organic material containing a large quantity of natural matter, for example, a fermentation of a strain of Lactobacillus strain vaccine compositions which can be obtained by reacting galacto plant extract.

Said galacto vaccine compositions which (Galactomyces) Is with america sesquisulphide proteins (Saccharomyces) As in at least one yeast, galacto said natural moisturising factor (NMF, natural moisturizing factor) fermentation vaccine compositions which comprises a is converted natural moisturising factor facilitates profilaggrin (profilaggrin) can be improve skin humectant. In addition, containing a polysaccharide component is minutely [su galacto said skin surface can be rich in prevent water evaporation.

Said Lactobacillus (Lactobaillus) Saccharide is generating energy as raw fermentation, Lactobacillus fermentation comprises said skin whitening effect is number billion pigmentation, skin inflammation can enhance skin immunity power.

Said Lactobacillus (Lactobaillus) Is Lactobacillus it buys k (Lactobacillus sakei B2 provided 16), Lactobacillus prevention (Lactobacillus brevis), Lactobacillus an earth other rum (Lactobacillus plantarum P23), Rhodopseudomonas Streptococcus lock teeth (Lactococcus lactis A164), bacillus [...] (Bacillus coagulance) Can be selected from the group consisting of at least one but, in vivo stability and fermentation lactic acid bacteria can be used one having excellent if not specially number.

In one in the embodiment, vaccine compositions which galacto said (Galactomyces) Comprising green tea leaf galacto vaccine compositions which can be fermented fermentation.

The popsicle said car reel [khin excellent antibacterial and anticancer mixtures, vitamin C, vitamin E, beta carotene, plant fiber, mineral and contains an enriched chlorophyll. Said popsicle antioxidant effect, an improvement effect of the skin elasticity, aging billion number effect, heavy metal number of special effect, anti allergic effect, effect for treating inflammation, immune activity enhancing effect on reported that holds a corrosion disclosed. In particular, skin care effect and an effect of said popsicle lotion minuteness [su galacto said elasticity enhancer can be be maximized by fermentation.

In one in the embodiment, said Lactobacillus (Lactobaillus) Comprising Lactobacillus fermentation product of fermentation can be germinated brown rice can be.

The germinated brown rice bran suitable oxygen at temperature and while preserving said inoculation in the sprout is come up, the sprout come up to 3 to 5 mm after growth generating or disclosure can be obtained by freeze-drying.

A germinating clothing in said skin-beneficial signal and various enzymes, vitamin B1, B2 when the frame sprout, B6, B12, Pantothenic acid, nicotinic acid, folic acid such as skin benefit ingredient can be enhanced remarkably. In addition, immune related components of a human body by the action of activated enzyme can be converted into material such as immunity with stipendiary thread column arabitol.

In one in the embodiment, said Lactobacillus (Lactobaillus) Fermentation are frequently dog place leaf, cabbage leaves, Ginseng fruit, and wheat germ further comprises one or more selected from the group consisting of Lactobacillus fermentation can be.

Said at least one Lactobacillus fermentation comprises Lactobacillus are switched by the synergistic effect of the effective component by means of Lactobacillus fermentation as compared to skin improving effect can be significantly improved.

Said dog place frequently (Medicago sativa) And the bay play the [cu galactose is such as to facilitate the high content of saccharides skin cell differentiation, K4 epidermal differentiation marker, such as CK19 profilaggrin and increase, anti-aging fibroblasts reported as good corrosion disclosed.

Said cabbage (Brassica) During growth as intermediate god lysine at amino acid rich, a large amount of vitamin C, potassium, calcium containing a JPO. In addition, the clear blood and of the resistance of the body lie on said cabbage, freckles, acne, other dermatological crushed flow tides and an excellent effect.

Ginseng fruit said (Panax ginseng C. A. Meyter) is an additional position lifestyle and referred (Ginseng berry) covalently, diameter about week 4 year lifestyle or more Ginseng heat opened [...] wall 7 are disclosed. Ginseng fruit said ginsenoside component and on hereinafter Ginseng roots, through in diabetic efficacy results superior near Ginseng exhibits the has been reported. In addition, retinoid treatment of inflammatory mediated factor number (retinoid) or surface-active agents such as Ginseng fruit said expression of skin irritation can billion number.

As a result of wheat flour would end it will know which number in the step for processing said wheat germ (wheat kernel) of about 2 to 3% whole wheat berry embryonic rich nutrients as occupy other. Said wheat germ is known tocopherol (vitamin) an antioxidant vitamin rich, natural substances extracted from wheat germ recovering damaged immune function of a human body to be wirelessly reports corrosion disclosed.

In one in the embodiment, cosmetic composition said licorice (Glycyrrhiza uralensis Fischer) Extract, state (Wolfiporia cocos) Extract, or [...] (Salvia Sclarea) Extract can be further.

"Extract" solvent extracted by said extraction material contacting under certain conditions contained material that defines the active ingredients of transition solvent, acids obtained by separating raw materials from natural material or components if extraction method can be used regardless of the type of.

Water or organic solvent extract dissolved in said solvent from the agricultural component extracting a, natural a particular constituent, for example certain components such as oil are extracted and the like can be obtained by including both.

In particular, said cosmetic composition containing the extract, therefore, thereby being useful for composite extract [...] and can be.

Each plant raw material due to the interaction between said active principles mixed extract skin improving effect can be substantially elevated, together with said at least one fermentation roh mote nicotinate derivatives may be peptides and acts upon the skin skin american pulse and wrinkle-reducing effect can be significantly enhanced.

In one in the embodiment, 10 to 20 parts by weight of said composite extract is present in the extract, 10 to 20 parts by weight of therefore, and 10 to 20 parts by weight of [...] extract can be.

Said composite extract ensures mixing ratio for each extract derived from whitening activity and improving active principles thereof can not implemented properly. In particular, a wide variety and type of active ingredient contained in said mixed extract according to the blending ratio of the mixed extract effect caused a concomitant application of which differ according to each extract (additive effect) is quickly move effect so 121b to be properly mixed [...] number.

In addition, in a process for checking residual quantity of said each kind of solvent does not specifically defined, it is can be various solvent is used. For example, said composition containing the, lower alcohols of 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1, 3 - butylene base material for one or more solvents selected from the group consisting can be extracted, said extraction process is said solvent leucorrhea needle, it comes the needle, heating or the like can be performed by a conventional method in the art.

In one in the embodiment, each plant raw material water washed and dried and ground to said extract, about 1 hr to 24 weight of 8 to 12 times each raw material where the volume of solvent extracting time filtered number bath 1308.

In addition, the pressure-reducing extract said additional process such as distillation or lyophilized powder by state can be obtained, including conventional mechanical process can extract number. For example, consistent molecular weight cut - off value said extract is present in the layer at a ultrafiltration, various chromatography (size, charge, hydrophobicity or affinity for the separation according to number as it became work) through various mechanical embodiment additionally apart by number method comprising fraction can be obtained.

In one in the embodiment, based on the overall composition relative to the weight of said cosmetic composition said fermentable matter and Lactobacillus fermentation galacto vaccine compositions which 1. 0 To 5. 0% By weight can be comprising.

Said fermentation amounts of water in 1. 0% By weight active ingredient is below said's can be fermented by inverted triangular screw, 5. 0% By weight in proportion to the added amount is greater than a pressure drop not effect is implemented cost-effective, since excessive force inherent within each said specific gravity fermentation product number can be cosmetic quality.

In one in the embodiment, be a cosmetic composition for skin whitening or said for wrinkle. It is sour with the mote but oh roh mote exhibits said cosmetic composition may be by skin whitening is superior antioxidant activity performance and number of the peptide number billion, and galacto said protein containing Lactobacillus fermentation treatment or vaccine compositions which, nucleic acid, fatty acid, vitamin, mineral or improving skin whitening and due to the synergistic effect of the two can be the first input stage. In particular said plant extract with said cosmetic composition when applied to skin improving effect can be be maximized.

In one in the embodiment, said cosmetic composition manufactured, nutritional skin water, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder selected from the group consisting of partially number 1308. elder brother anger.

Said cosmetic composition in said number type or the type of intended use in addition each number type essential not according to the present invention according to purposes by suitably mixing different components within a range that can be.

In addition, an imaginary number said final cosmetic composition according to industry or function typically used fatty substance, organic solvent, dissolved number, concentrated number, gelling number, softening number, antioxidant number, number of suspension, stabilizing number, foam (foaming agent) number, direction number, number surfactants, water, -glucose or nonionic emulsion number, charging number, metal ion seal chain number, chelating number, preserving number, blocking number, apply a number, essential oil, dye, pigment, hydrophilic or lipophilic active number, or any other commonly used cosmetic ingredients normally used in dermatological field cosmetics such as Cu or auxiliary can be additionally contains a number.

But, the present invention according to said auxiliary number cosmetic composition and said mixing ratio can be appropriately selected so as to avoid an influence desirable properties.

Through hereinafter in the embodiment, the present invention in the embodiment for the present invention by a number not one but by the above-mentioned further non nontrivial disclosed.

1 Attainments number: - roh mote nicotinate (YGGFM) number of bath may be pen hit [pheyp tie [tu

The second flue 9 - pen hit [pheyp tie five [ley neel mauls [thok hour carbonyl (9 a-fluorenylmethoxycarbonyl; Fmoc) solid phase peptide synthesis using a conventional dab of amino acids (solid phase peptide synthesis: SPPS) synthesis using a transparent conductive layer, N - hydroxy benzo triazole (N-a hydroxybenzotriazole; HOBt) on N, N - diimide (N, N' - diisopropylcarbodiimide; DIC) is activated by using the method of small pro car step which will bloom number extending amino acid residue his [reference document: Wang C. Chan, Perter D. White, 'Fmoc-a solid phase peptide synthesis', Oxford].

NH synthesized₂ 20% Piperidine/NMP (YGGFM) - protected peptide resin to the Fmoc amino group and coupled to a stand-alone solution to the number, N - methyl - 2 - pyrrolidone (dichloromethane: DCM) dichloro methane (N a-methyl-a 2 a-pyrrolidone: NMP) on nicotinic acid (nicotinic acid) in 12 hours at room temperature after washing 5 equivalent with respect to the coupling reaction. After that NMP is washed and very dry DCM reaction on many times.

Pen hit [pheyp tie [tu - - resin with a styrene thermoplastic rubber composition may be dried nicotinate roh mote: phenol: thio it knows the brush: water: ethane d mote this year (82. 5:5: 5:5: 2. 5 (V/v)) mixed solution reacting 2 to 3 hours at room temperature, amino acid residues peptide t - butyl-oxycarbonyl (Boc) groups present in a side chain protecting group of the bondable functionality may be a stand-alone number are arranged after the separation of the pen hit [pheyp tie [tu - roh mote and exhibits, a precipitation [...] said low with respect to the peptide.

0 - Roh mote nicotinate derivatives obtained may be pen hit [pheyp tie [tu. 1% Styrene thermoplastic rubber composition includes water and process for the reverse phase high performance liquid chromatography (reverse phase high performance liquid chromatography; column: Gemini, C18 110A 250 × 21. 2 Mm) (yield: 65%) was a positive number.

2 Attainments number: - nonapeptides (YGGFLRKYP) number of bath may be roh mote nicotinate

A method said in the embodiment 1 according to the NH₂ - (YGGFLRKYP) - protected peptide resin copiers.

Said synthesized NH₂ 20% Piperidine/NMP (YGGFLRKYP) - protected peptide - coupled to a stand-alone solution to the Fmoc amino resin to the number and, after washing the nicotinic acid (nicotinic acid) in NMP at room temperature 12 hours on the DCM 5 equivalent with respect to the coupling reaction. After that NMP DCM and dried cleaning has been many times on the reaction.

- Resin with a styrene thermoplastic rubber composition may be dried nicotinate roh mote - nonapeptides: phenol: thio it knows the brush: water: ethane d mote this year (82. 5:5: 5:5: 2. 5 (V/v)) mixed solution reacting 2 to 3 hours at room temperature, amino acid residues peptide t - butyl-oxycarbonyl (Boc) groups present in a side chain protecting group of the bondable functionality may be a stand-alone are arranged after the separation of the number and it plays but [pheyp tie [tu - roh mote nicotinate, low temperature [...] been said to precipitate the peptide.

0 - Roh mote nicotinate derivatives obtained may be pen hit [pheyp tie [tu. 1% Styrene thermoplastic rubber composition includes water and process for the reverse phase high performance liquid chromatography (reverse phase high performance liquid chromatography; column: Gemini, C18 110A 250 × 21. 2 Mm) (yield: 62%) was a positive number.

3 Attainments number: popsicle galacto vaccine compositions which number tank fermentation

Glucose, sugar, glutinous, yeast extract (yeast extract) included in the fermentation medium (glucose 1. 5% (V/v), sugar 1. 5% (V/v), glutinous 1. 5% (V/v), yeast extract 0. 3% (V/v)) using galacto foam (Galactomyces) By fermentation product number into his high pressure liquid coolant.

Said medium (10 ml) was added to 10 mg amount (pre-a cultivation) pre-dried enzyme foam galacto to. The composition of the fermentation medium equal to said adding said extract with respect to the fermentation medium popsicle [...] pre-seeding.

36 Into said galacto Streptomyces strains. 5 °C 36 has been cultured in time, in order to increase the sugar content and its galacto foam (Galactomyces) culture was exposed under [e number. After fermentation is completed fermentation vaccine compositions which can be obtained by centrifugation and filtering galactosidase been.

4 Attainments number: wheat germ galacto vaccine compositions which number tank fermentation

The same method is high pressure liquid coolant to said number 3 attainments fermentation product number, instead of wheat germ extract green tea leaf extracts of some excellent.

5 Attainments number: Lactobacillus fermentation tank number germinated brown rice

2% Trip tone, 1% yeast extract, 2% six extracts, 0. 2% K2HPO 4, 0. 4% Tree ammonium citrate, 0. 5% Acetic acid sodium, 0. 1% Tween 80, 0. 028% MnSO4, 5H2O, 0. 058% MgSO4, 7H2O, 0. 074% CaCl2, 2H2O, 10% suspension in 30 minutes after fermentation medium number number number 120 °C atrough-de-sterilizing his high pressure liquid coolant.

Said fermentation medium in anaerobic conditions is provided which reduces the amount pre-dried polyester strain 20 mg-gate. The composition of the fermentation medium equal to said medium, said pre-germinated brown rice extract was added to achieve an [...].

30 To 40 °C 36 to 72 hours has been cultured in polyester said strains, after fermentation is completed by centrifugation and filtering Lactobacillus fermentation has been obtained.

6 Attainments number: cabbage leaf number Lactobacillus fermentation tank

The same method is high pressure liquid coolant to said number 3 attainments fermentation product number, instead of cabbage leaf extracts of the germinated brown rice extract some excellent.

7 Attainments number: number Ginseng fruit Lactobacillus fermentation tank

The same method is high pressure liquid coolant to said number 3 attainments fermentation product number, Ginseng extract some excellent fruit extracts germinated brown rice.

8 Attainments number: number of functional bath

Licorice, therefore, it will grow, washed after each obtained by finely grinding 100g completely drying at room temperature.

Each compacted 100g respect to 10 times of ethanol aqueous solution (50% mass concentration) by 12 hours at a temperature of 50 °C solvent extracted from the deposited. Each extract after filtering by a filter, the method that is the same for the respective residual raw material by mixing high pressure liquid coolant and is repeated 3 times extract composite extract his number.

Reducing pressure in the concentrated within said composite extract 30 °C, lyophilized solid material is obtained.

In the embodiment and comparison example

Table 1 peptide containing a composition ratio may be roh mote nicotinate sample number according to his high pressure liquid coolant.

The composition of table 2 elder brother anger component to said number such as disclosed.

Experiment example 1: cytotoxic evaluation

In the embodiment and comparison sample embodiments the cell toxicity were measured. A deformation in said in the embodiment and comparison sample number number obtained by concentration by suspension (100, 50, 25 μg/mL) is composed, a method to form a pavement cells via survival rates were measured.

{3 - (4, 5 A-dimethylthiazol-a 2 a-yl) - 2 provided 5 a-diphenyltetrazolium bromide} MTT reagent (Mosmann) method of measuring cells via survival rates MOS only by deforming the cytotoxic were measured.

HDF cells 1 × 10 96 a-well plate (Human Dermal Fibroblast)⁴ 37 °C by dividing the concentration of cells/well, 5% CO₂ With respect to the stabilizing in 24 hours.

Said sample concentration 24 hours obtained by treating him as. After 2 hours 5 mg/mL MTT concentration into a medium number him as a stand-alone. A DMSO (Dimethyl sulfoxide) by adding 100 μl, 10 minutes been rapidly in absorbance 540 nm were measured.

The measurement result, the positions of each cytotoxic concentrations him money. Said peptide exhibits characteristics may result roh mote, galacto vaccine compositions which fermentable matter, Lactobacillus fermentation are excellent safety to human clinical use other.

Experiment example 2: collagen proliferation effect experiments

After culturing fibroblast feeder cells (primary cell line, CCD provided 986sk) inoculating the bottom plate is penicillin-resistant Streptococcus pneumoniae (100 IU/ml), Streptomyces feed (100 micro g/ml) and 10% FBS (fetal bovine serum) containing DMEM (Dulbecco's Modified Eagle's Medium) was cultured in 5% carbon dioxide incubator including 37 °C into and out of the holds.

48 Well culture plate cells per well after two divider 5X104 cloning, cell culture conditions him as 24 hours. 10% PBS (phosphate buffered saline) or of special medium number after said in the embodiment 1 to 8 and washed, comparison example 1 to 4 after processing him as time sample 24. Table 1 number of experiments for accuracy of sealer is coated in the elder brother anger component number water for him.

Pro-collagen type I C - peptide (PIP) taken culture (procollagen type) (Takara, MK101) collagen amount were measured by ELISA.

Adding a 5 to 1 dilution to 100 micro l - PoD antibody conjugates solution well 20 in a liquid culture medium and introducing micro l 37 °C 3 adding him as time. L PBS 400 after culture well and in a stand-alone micro-wash up to number 4. After 15 minutes at room temperature into and out of the cell culture reagents 100 micro l, 1N in ELISA reader (reader) to 450 nm into and out of the 100 sulfate micro l were measured.

Collagen proliferation effect has a positive number number of absorbance controls (100%) have been calculated based on, for table 3 have shown to result.

Experiment example 3: skin for improving brightness evaluation

In the embodiment 1 to 8, 1 to 4 comparison example cosmetic sample skin effect brightness was assessed.

20 To 40 year 30 name for using predetermined during test after measuring subject brightness Chromameter CR provided 400 before use of skin in the embodiment and comparison sample embodiments of cosmetic skin individually re-brightness were measured.

A result of the determination is to table 4 precursor, L * 3 average was calculated measuring value is a maximum minimum number [...] Conference.

the reference table 4, in the embodiment 1 to 8 compared to the comparison example 1 to 4 pixel value differences is the skin by cosmetic sample has been remarkably improved.

While, comparison example 1 to 4 cosmetic sample before and during use brightness difference not relatively large, the adsorption fixing plate 105a his skin and increased brightness.

I.e., nicotinate derivatives may be roh mote peptide, fermentable matter galacto vaccine compositions which, in the embodiment 1 to 8 Lactobacillus fermentation product containing cosmetic skin improving effect on brightness when the sample is excellent, in particular mixed extract and at least one Lactobacillus fermentation sample cosmetic skin for improving brightness effect and including in the embodiment 3, 4, 6, 7 are improved.

Experiment example 4: skin moisturizing effect evaluation

In the embodiment 1 to 8, 1 to 4 comparison example cosmetic sample skin moisturizing effect was assessed.

20 To 40 year Chromameter CM825 test using a subject during predetermined 20 name for skin moisturizing amount before use of measuring skin moisturizing amount material individually in the embodiment and comparison relate to cosmetic composition were measured.

A result of the determination to a table 5 precursor, a minimum value and a maximum average was calculated number [...] Conference 3 measurements.

the reference table 5, in the embodiment 1 to 8 compared to the increasing amount of cosmetic sample comparison example 1 to 4 moisturizing the skin by rate significantly looking up.

While, comparison example 1 to 4 cosmetic preparation containing a relatively large degree before and during use of moisturizing the amount of sample is not improved, the adsorption fixing plate 105a his skin moisturizing effect.

I.e., nicotinate derivatives may be roh mote peptide, galacto vaccine compositions which fermentable matter, including in the embodiment 1 to 8 Lactobacillus fermentation product sample is under excellent cosmetic skin moisturizing effect on been. In particular mixed extract and at least one Lactobacillus fermentation product including in the embodiment 3, 4, 6, 7 cosmetic sample is more skin moisturizing effect are improved.

Experiment example 5: the skin elasticity improvement effect evaluation

In the embodiment 1 to 8, 1 to effect cosmetic skin sample comparison example 4 was assessed.

20 Cells or more healthy female 80 name (average age 37 cells) where the divided into 8, 24 to 26 °C temperature, humidity 75% skin-elasticity conditions were measured.

1 To 8 in the embodiment 1 up to the group 8 group, comparison example 1 to 4 each applied cosmetic sample. About 2 times per day (morning and evening) by said cosmetic sample eye rim 12 when the daytime applying, measuring the skin elasticity (Cutometer MPA580, yarn Conrage + Khazaka, Germany federation country) using resilient skin portion of a subject for a long time. 　

Test results of cutometer MPA580 R8 (R8 (12 main) - R8 (0 main)) value are described. Said R8 value (Vicoelasticity) exhibit viscoelastic properties of the skin, to table 6 results such as disclosed.

As shown in the table 6, in the embodiment 1 to 8 in comparison with comparison example 1 to 4 skin cosmetic sample effect significantly transmission option.

Experiment example 6: skin wrinkle effect evaluation

In the embodiment 1 to 8, 1 to 4 comparison example skin wrinkle effect cosmetic sample was assessed.

30 Female 80 name the person under test to a test object 8 divided into three or more adult skin wrinkle were measured. PC [hem it sleeps during main 4 every morning, evening after cleansing cosmetic sample from the patient to allow said base resin absorbent with respect to the face.

ANTERA 3D (Miravex, Ireland) assess wrinkle using a transparent conductive layer, using said sample prior to and after use (after main 12) point in time were measured.

The same test on all persons have left the person under test measuring [...] site, measurement reproducibility is measured before use test substance for the same site overlapping the Image were measured. A picked-up Image ANTERA 3D ANTERA pro software matching using special software analyzing his site after a consistent.

Measuring a variable Indentation index representing Wrinkles small value measurements using skin electrodes respectively. Table 7 in the embodiment and comparison using the person under test according to embodiments of cosmetic sample in Wrinkles small value of variation (main 4 after a lapse) of intetrest. I.e., excellent Wrinkles small value and the real increase that a side surface of the substrate. A minimum value and a maximum average was calculated number [...] Conference 3 measurements.

the reference table 7, comparison example 1 to 4 in the embodiment 1 to 8 or improving skin cosmetic sample of cosmetic composition as compared to significantly transmission option. In particular, mixed extract and at least one Lactobacillus fermentation product including in the embodiment 3, 4, 6, 7 in the embodiment in comparison with other cosmetic sample skin wrinkle effect and are improved.

Experiment example 7: improving evaluation amount melanin

A cosmetic composition acryl elder brother anger said number in accordance with predetermined criteria using a name of an 80 degree improving ANTERA 3D divided into 8 woman as a melanin amount was assessed.

The person under test when the same test on all persons left the measuring device may, for cosmetic compositions measurement reproducibility of the measuring Image overlapping same site is measured. After the matching using a picked-up Image before ANTERA 3D software ANTERA pro software for analyzing site was consistent. When the measuring value is indicative of the amount of melanin melanin strobe, device measurements are cosmetic compositions before and 4 main at the passage point in the backplane.

In the embodiment 1 to 8 is to table 8, the person under test according to comparison example 1 to 4 cosmetic sample exhibits melanin value used is detected after main 4. I.e., the side surface of the melanin value increase melanin amount effective in diminishing the real substrate. A minimum value and a maximum average was calculated number [...] Conference 3 measurements.

With reference to the table 8, in the embodiment 1 to 8 cosmetic sample melanin amount that a transmission option. In particular, in particular, in the embodiment cosmetic sample mixed extract and at least one Lactobacillus fermentation product including in the embodiment 3, 4, 6, 7 other relatively in comparison with the amount of melanin that a more transmission option.

In case that the description of the invention which is for example, of the present invention technical idea of the present invention is provided to a person with skill in the art or essential characteristics without changing other form may be understand easily outputted are disclosed. In the embodiment described above the exemplary non-limiting all sides are understood to which must substrate. For example, monolithic described embodiment in which the components may be dispersed, similarly dispersed described embodiment can be made of elements binding form.

Claims range of the present invention are represented by carry, therefore the meaning of the general outline of the form of the present invention evenly and range and method for some all changing or modified range should interpreted.