METHODS AND RELATED DEVICES FOR SINGLE MOLECULE WHOLE GENOME ANALYSIS

Provided are methods of labeling and analyzing features along at least one macro molecule such as a linear biopolymer, including methods of mapping the distribution and frequency of specific sequence motifs or the chemical or proteomic modification state of such sequence motifs along individual unfolded nucleic acid molecules. The present invention also provides methods of identifying signature patterns of sequence or epigenetic variations along such labeled macro molecules for direct massive parallel single molecule level analysis. The present invention also provides systems suitable for high throughput analysis of such labeled macro mo lecules. 1. A method of analyzing a double-stranded DNA sample , comprising:processing a double-stranded DNA sample so as to give rise to a flap of the first strand of the double-stranded DNA sample being displaced from the double-stranded DNA sample,the flap having a length in the range of from about 1 to about 1000 bases, andthe flap giving rise to a gap in the first strand of the double-stranded DNA sample corresponding to the flap;incorporating one or more bases into the double-stranded DNA so as to eliminate at least a portion of the gap;labeling at least a portion of the processed double-stranded DNA with one or more tags; andcorrelating the location of one or more labels to a structural characteristic of the DNA sample.2. The method of claim 1 , wherein the processing comprises nicking the first strand of double-stranded DNA at one or more sequence-specific or sequence non-specific locations.3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. The method of claim 1 , wherein the flap generation is modulated by polymerase extension claim 1 , by incorporation of one or more nucleotides claim 1 , by reaction time claim 1 , by the presence of a reaction terminator claim 1 , or by any combination thereof.8. (canceled)9. (canceled)10. (canceled)11. (canceled)12. The method of claim 1 , wherein the labeling is effected by binding ...

NANOCHANNEL ARRAYS AND NEAR-FIELD ILLUMINATION DEVICES FOR POLYMER ANALYSIS AND RELATED METHODS

Provided are devices and methods for polymer analysis, in which labeled polymers are translocated along nanochannels that have illuminated detection regions within. As a labeled polymer translocates along the nanochannel and the labels pass over the illuminated detection regions, the labels become detectable (e.g., where different labels fluoresce in response to different wavelengths present at different illumination regions), and the user generates a spatial map of the location of the various labels along the length of the polymer. The location of the labels is then correlated to one or more structural characteristics of the polymer. 1. An analysis device , comprising:a plurality of nanochannels running along the surface of a first substrate, each nanochannel defined by side walls and a floor; andat least one nanoslit being disposed in a second substrate, the nanoslit being disposed such that electromagnetic radiation of at least a first wavelength passing through the nanoslit defines, within the at least one nanochannel, an illumination region having a cross-sectional dimension smaller than the first wavelength.2. The analysis device of claim 1 , wherein the first substrate comprises silica claim 1 , silicon claim 1 , dielectrics claim 1 , insulators claim 1 , glass claim 1 , borofloat glass claim 1 , sodalime glass claim 1 , silicon oxynitride claim 1 , SiON claim 1 , hydrogenated silicon dioxide claim 1 , hydrogenated silicon nitride claim 1 , hydrogenated silicon oxynitride claim 1 , high K dielectrics claim 1 , compounds comprising titanium: TiSiO claim 1 , TiO claim 1 , TiN claim 1 , titanium oxides claim 1 , hydrogenated titanium oxides claim 1 , titanium nitrides claim 1 , hydrogenated titanium nitrides claim 1 , TaO claim 1 , TaSiO claim 1 , TaON claim 1 , TaO claim 1 , TaCN claim 1 , tantalum oxides claim 1 , hydrogenated tantalum oxides claim 1 , tantalum nitrides claim 1 , hydrogenated tantalum nitrides claim 1 , compounds containing hafnium: Hf0 claim ...

POPPET VALVE ASSEMBLY FOR CONTROLLING A PNEUMATIC ACTUATOR

A poppet valve assembly to control a pneumatic actuator may include a housing having a central bore. A first module may be disposed within the central bore, and the first module may have a first and second poppet valve. A second module may also be disposed within the central bore, and the second module may have a third and fourth poppet valve. A supply of pressurized fluid may be in fluid communication with a plurality of control valves such that pressurized fluid from the control valves opens and closes the poppet valves. The supply of pressurized fluid may also be in fluid communication with the first module and the second module such that the opening and closing of the poppet valves controls the position of the pneumatic actuator. 1. A poppet valve assembly comprising:a valve housing having a central bore;a first module disposed within the central bore, the first module comprising a first normally closed poppet valve and a second normally closed poppet valve, wherein each of the first and second normally closed poppet valves has an open position and a closed position;a central port formed in the first module, wherein the central port of the first module is adapted to be coupled to a first volume of a pneumatic cylinder;an exhaust port formed in the first module such that the central port is in fluid communication with the exhaust port when the first poppet valve is in the open position; anda supply port formed in the first module such that the central port is in fluid communication with the supply port when second poppet valve is in the open position,wherein the supply port is configured to be in fluid communication with a supply of pressurized fluid such that when the second poppet valve is in the open position, pressurized fluid is provided to the first volume of the pneumatic cylinder, andwherein the exhaust port is configured to vent pressurized fluid from the first volume of the pneumatic cylinder when the first poppet valve is in the open position.2. The ...

VALVE SIGNATURE DIAGNOSIS AND LEAK TEST DEVICE

A valve signature diagnosis and leak testing device includes a spool valve operatively connected to a pilot valve, the pilot valve being configured to position the spool valve to one of an open position and a closed position. A blocker valve is fluidly connected to a control fluid outlet of the spool valve. An electrical module is operatively connected to the pilot valve, a supply of control fluid, and the blocker valve, the electrical module being capable of sending pulsed electrical signals to the pilot valve and the blocker valve to selectively position the spool valve and the blocker valve to an open or closed position. 1. A valve signature diagnosis and leak testing device for a control valve , the valve signature diagnosis and leak testing device comprising:a spool valve operatively connected to a pilot valve, the pilot valve being configured to position the spool valve to one of an open position and a closed position, the spool valve including a first control fluid inlet, a first control fluid outlet, and a second control fluid outlet, the first control fluid inlet being fluidly connected to a supply of control fluid and the first control fluid outlet being configured to be connected to a valve actuator;a blocker valve fluidly connected to the second control fluid outlet; andan electrical module operatively connected to the pilot valve, the supply of control fluid, and the blocker valve, the electrical module being capable of sending pulsed electrical signals to the pilot valve and the blocker valve to selectively position the spool valve and the blocker valve to an open or a closed position,wherein the open position of the spool valve fluidly connects the first control fluid inlet to the first control fluid outlet and the closed position of the spool valve fluidly connects the first control fluid outlet to the second control fluid outlet.2. The valve signature diagnosis and leak testing device of claim 1 , wherein the electrical module includes a main ...

WIRELESS MONITORING AND CONTROL OF SAFETY STATIONS IN A PROCESS PLANT

A safety station for use in a process plant includes one or more leverless limit switches to detect activation of one or more parts of the safety station. The safety station further includes a wireless transmitter coupled to the leverless limit switches to transmit signals associated with the safety station to a base station device, which is communicatively coupled to one or more control and/or monitoring devices. 1. A safety station for use in a process plant , the safety station comprising:one or more leverless limit switches to detect activation of one or more parts of the safety station; anda wireless transmitter coupled to the leverless limit switches to transmit signals associated with the safety station to a base station device, wherein the base station device is communicatively coupled to one or more control and/or monitoring modules.2. The safety station of claim 1 , wherein the leverless limit switch is a GO® switch manufactured by the TopWorx corporation.3. The safety station of claim 1 , wherein the leverless limit switch remains latched until physically reset.4702. The safety station of claim 1 , wherein the wireless transmitter is the Rosemount dual input transmitter manufactured by the Emerson corporation.5. The safety station of claim 1 , wherein the wireless transmitter is an intrinsically safe wireless transmitter.6. The safety station of claim 1 , wherein the safety station is a safety shower and/or an eye wash station.7. The safety station of claim 1 , wherein at least one of the one or more control and/or monitoring stations is a remote touch screen panel.8. The safety station of claim 1 , wherein at least one of the one or more control and/or monitoring stations is a paperless recorder.9. The safety station of claim 1 , wherein at least one of the one or more control and/or monitoring stations is a workstation.10. A safety station monitoring and control system in a process plant claim 1 , the system comprising:one or more safety stations ...

SWITCH MODULE

A switch module used for position sensing may be operated in a number of modes for compatibility with a number of legacy position sensing products. A dry switch circuit can be configured to provide a direct output, emulating dry reed, high-side or low-side switched configurations. Alternatively, the dry switch circuit can be connected to an input of a NAMUR circuit to provide the known current output for that standard. In another configuration the dry switch can be selectively coupled to one of two NAMUR circuits allowing the switch module to provide a low-to-high current NAMUR output or a high-to-low current NAMUR output. 1. A switch module arranged and adapted to report a position of a moveable element external to the switch module , the switch module comprising:a first external connection, a second external connection, and a third external connection;a dry contact relay having a relay contact position responsive to a position of a component external to the switch module, the dry contact relay having a common connection, a normally open connection and a normally closed connection, where the normally open connection corresponds to a first position of the moveable element and the normally closed connection corresponding to a second position of the moveable;a NAMUR circuit having a first input connection pair and a first output connection pair;an electrical connection from the normally open connection of the dry relay to a first input of the first input connection pair of the NAMUR circuit;a selector operable to connect the common connection of the dry contact relay to i) a null contact of the selector or ii) a second input of the first input connection pair of the NAMUR circuit;a second electrical connection from the first output of the NAMUR circuit to one of the first external connection or the second external connection; anda third electrical connection between the second output of the NAMUR circuit to the third external connection;a first jumper that removeably ...

PHYSICAL MAP CONSTRUCTION OF WHOLE GENOME AND POOLED CLONE MAPPING IN NANOCHANNEL ARRAY

Methods for generating physical maps for polynucleotides, such as genomic DNA, are disclosed herein. Also disclosed are methods for identifying the source of polynucleotides. The methods can, for example, be used in physical map construction of whole genome. In addition, methods and systems capable of performing high throughput characterization of macromolecules using nanofludic devices are enclosed. 1. A method for identifying the source of polynucleotides , comprising:providing a plurality of biological samples wherein each of the plurality of biological samples comprises a polynucleotide;combining the plurality of biological samples in a plurality of pools, wherein each biological sample is present in at least two pools;obtaining structural information of the polynucleotides present in each pool; andassigning the polynucleotides to corresponding biological samples using the structural information obtained for the polynucleotides.2. The method of claim 1 , wherein the biological samples comprise randomly sheared or restriction enzyme generated polynucleotide fragments or such fragments carried in plasmids claim 1 , fosmids claim 1 , cosmids claim 1 , viral vectors claim 1 , artificial chromosome clones claim 1 , or any combinations thereof.3. The method of claim 2 , wherein the artificial chromosome clones are Bacterial Artificial Chromosomes claim 2 , Yeast Artificial Chromosomes claim 2 , or any combinations thereof.4. The method of claim 2 , wherein each of the polynucleotide fragment present in the artificial chromosomes is a fragment of a genomic DNA.5. The method of claim 1 , wherein the obtaining structural information of the polynucleotides comprises sequencing at least a portion of the polynucleotides.6. The method of claim 1 , wherein the obtaining structural information of the polynucleotides compriseslabeling the polynucleotides present in each pool, andlinearizing, in a nanochannel fluidic device, at least a portion of the labeled polynucleotides.7. ...

METHODS AND RELATED DEVICES FOR SINGLE MOLECULE WHOLE GENOME ANALYSIS

Provided are methods of labeling and analyzing features along at least one macromolecule such as a linear biopolymer, including methods of mapping the distribution and frequency of specific sequence motifs or the chemical or proteomic modification state of such sequence motifs along individual unfolded nucleic acid molecules. The present invention also provides methods of identifying signature patterns of sequence or epigenetic variations along such labeled macromolecules for direct massive parallel single molecule level analysis. The present invention also provides systems suitable for high throughput analysis of such labeled macromolecules. 139-. (canceled)40. A method for analyzing a double stranded DNA , comprising:nicking one strand of the DNA at a nick site;labeling the DNA at or about the nick site;ligating the labeled DNA with a ligase; anddetecting the label.41. The method of claim 40 , wherein said nicking is accomplished with a site-specific nicking enzyme.42. The method of claim 41 , wherein said nicking claim 41 , labeling claim 41 , ligating claim 41 , and detecting are each performed at multiple sites on the DNA.43. The method of claim 42 , further comprising transporting the ligated DNA into a nanochannel and maintaining the DNA in elongated form in the nanochannel.44. The method of claim 40 , wherein the label is fluorescent.45. The method of claim 40 , wherein the label is a fluorescently-labeled base.46. The method of claim 40 , wherein after said nicking the DNA has a break in a single strand claim 40 , into which at least one nucleotide is introduced.47. The method of claim 46 , wherein said nick separates first and second pieces of the nicked strand and wherein prior to said ligating said at least one nucleotide is joined to said first piece but not to said second piece.48. The method of claim 46 , wherein said at least one nucleotide is labeled.49. The method of claim 48 , further comprising transporting the labeled DNA into a nanochannel ...

METHODS AND DEVICES FOR SINGLE-MOLECULE WHOLE GENOME ANALYSIS

Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals. 158-. (canceled)59. A method of characterizing a DNA , comprising:labeling a plurality of sequence-specific locations on a first DNA with a labeling technique that does not cut DNA;linearizing at least a portion of the labeled first DNA;detecting a recognizable pattern of labels on the linearized first DNA, wherein the labels are indicative of a sequence characteristic of the first DNA; andcomparing the recognizable pattern of labels on the linearized first DNA to a pattern of labels indicative of a known sequence characteristic of a reference DNA to ascertain a similarity or difference in a sequence characteristic of the first DNA and the reference DNA.60. The method of claim 59 , further comprising:labeling a plurality of sequence-specific locations on a second DNA with a labeling technique that does not cut DNA;linearizing at least a portion of the second DNA;detecting a recognizable pattern of labels on the linearized second DNA, wherein the labels are indicative of a sequence characteristic of the second DNA; andcomparing the recognizable patterns of the first and second DNAs to ascertain a similarity or difference in a sequence characteristic of the first and second DNAs.61. The method of claim 60 , wherein the first and second DNAs are each double stranded.62. The method of claim 61 , wherein the labeling is effected with a methyltransferase claim 61 , a non-cutting restriction enzyme claim 61 , a zinc finger protein claim 61 , an antibody claim 61 , a transcription factor claim 61 , a DNA binding protein claim 61 , a hairpin polyamide claim 61 , a triplex-forming oligodeoxynucleotide claim 61 , a peptide nucleic acid claim 61 , or a combination thereof.63. ...

SYSTEMS AND METHODS FOR ASSESSING BIOMOLECULE CHARACTERISTICS

Provided are methods and systems for assessing the presence and extent of damage on a polynucleotide. The methods include incorporating a label at the site of the damage and imaging the label to determine the presence and extent of the damage. The systems include devices capable of performing damage assessment on single molecules. 192-. (canceled)93. An analysis system , comprising:a sample stage configured to receive a fluidic chip, wherein the fluidic chip comprises one or more nanochannels having at least one dimension in a range of from about 1 nm to about 250 nm,two or more illumination sources configured to illuminate a sample disposed within the fluidic chip, wherein each illumination source is configured to provide a different illumination wavelength; andan image collector configured to collect one or more images of an illuminated sample disposed within the fluidic chip.94. The system of claim 93 , further comprising a detector capable of detecting positions of one or more beams of illumination reflected from the sample disposed within the fluidic chip.95. The system of claim 93 , further comprising a controller configured to translate the sample stage in response to a position of a beam of illumination reflected from the fluidic chip.96. The system of claim 93 , further comprising at least one filter capable of being disposed in an illumination path so as to provide a desired illumination wavelength to the sample disposed within the fluidic chip.97. The system of claim 93 , further comprising a beam expander disposed in an illumination path between the illumination source and the sample.98. The system of claim 93 , wherein the system comprises a source of electric field configured to move one or more macromolecules into nanochannels of the fluidic chip.99. The system of claim 93 , further comprising a reader configured to recognize the system in accordance with one or more indicia disposed on the fluidic chip.100. The system of claim 93 , wherein the system ...

Methods of determining nucleic acid structural information

Methods of double-stranded nucleic acid sequence determination and assembly that are able to identify insertions, deletions, repeat region sizes and genomic rearrangements, for example, are disclosed herein, which can use relatively large labeled nucleic acid fragments to analyze the structure of even larger genetic regions. In some embodiments these methods involve the use of certain parameters which unexpectedly improve overall method performance. In some embodiments these methods involve sample labeling that does not result in the formation of single-stranded nucleic acid fragment labeling intermediaries.

Isolation of megabase-sized dna from plant and animal tissues

Disclosed herein are methods for isolation of long DNA molecules, for example megabase-sized genomic DNA molecules, from a biological sample, for example plant and animal tissues.

CONFIGURABLE PROCESS CONTROL DEVICE WITH ELECTRONIC DISPLAY ASSEMBLY

A configurable process control device, which includes a field device, such as a valve position controller, that can be configured by a user to emulate any one of a plurality of different types of process control devices, is provided with an electronic display assembly. The electronic display assembly is operatively connected with a control circuit that is arranged to respond to the specific configuration of the field device to cause the electronic display assembly to display information relevant to the specific type of control device the field device has been configured to emulate. The information may include safety certification information specific to each of the different types of process control devices that the field device can be configured to emulate. 1. A configurable process control device for use in a plurality of use environments , wherein at least one of a pre-defined plurality of certifications must be displayed in conjunction with the configurable process control device depending on which of the use environments the configurable process control device is to be used in , the configurable process control device comprising:a field device;a first control circuit operatively coupled to the field device, the first control circuit arranged to control the field device and arranged to be reconfigured by a user to allow the field device to selectively emulate any of a plurality of different process control device types;an electronic display assembly, the electronic display assembly arranged to selectively display different information; anda second control circuit, the second control circuit operatively coupled to the electronic display assembly and the first control circuit, the second control circuit having access to a plurality of different certification information sets, each certification information set corresponding to a different one of the plurality of different process control device types;wherein the second control circuit causes the electronic display ...

PIEZOELECTRIC LOUDSPEAKER

The present invention discloses a piezoelectric loudspeaker. The piezoelectric loudspeaker comprises a sound producing plate, a resonant sound-box, a surround and a reflective sound-box. The sound producing plate comprises a piezoelectric ceramic element. The resonant sound-box includes a first opening comprising a first carrying part. The sound producing plate is disposed on the first carrying part. A cavity resonator is formed between the sound producing plate and the resonant sound-box. The surround is disposed between the first carrying part and the sound producing plate. The reflective sound-box includes a second opening and a reflective output opening. The second opening comprises a second carrying part. The resonant sound-box is disposed on the second carrying part. A reflective cavity body is formed between the resonant sound-box and the reflective sound-box, and the reflective cavity body is connected the reflective output opening. 1. A piezoelectric loudspeaker , comprising:a sound producing plate including at least one piezoelectric ceramic element being able to vibrate the sound producing plate;a resonant sound-box including a first opening comprising a first carrying part, the sound producing plate being disposed on the first carrying part, and a cavity resonator being formed between the sound producing plate and the resonant sound-box;a surround disposed between the first carrying part and the sound producing plate; anda reflective sound-box including a second opening and a reflective output opening, a second carrying part formed on a partial of an inner edge of the seconding opening, the resonant sound-box being disposed on the second carrying part, a reflective cavity body being formed between the resonant sound-box and the reflective sound-box, the reflective output opening being disposed on a side of the reflective sound-box, and the reflective cavity body being communicating with the reflective output opening.2. The piezoelectric loudspeaker of ...

METHODS FOR SINGLE-MOLECULE ANALYSIS

Methods for single-molecule preparation and analysis are disclosed herein. The methods can, for example, be used for isolating and analyzing DNA from various biological samples. 136-. (canceled)37. A method of characterizing a DNA , the method comprising:nicking a first DNA at a first sequence motif, wherein the first DNA is double stranded, and wherein the first DNA remains double-stranded adjacent to the nicks;labeling the nicks on the first DNA with a first label;marking the labeled first DNA with a third label, wherein the third label is non-sequence-specific, and wherein the third label is different from the first label;linearizing the first DNA following labeling with the first and third labels; anddetecting the pattern of the first label on the linearized first DNA.38. The method of claim 37 , further comprising repairing the nicks on the first DNA.39. The method of claim 37 , further comprising:nicking a second DNA at the first sequence motif;labeling the nicks on the second DNA with the first label;repairing the nicks on the second DNA;marking the repaired second DNA with the third label;linearizing the second DNA following labeling with the first and third labels; anddetecting the pattern of the first label on the linearized second DNA.40. The method of claim 37 , further comprising:nicking the repaired first DNA at a second sequence motif, wherein the repaired first DNA remains double-stranded adjacent to the nicks;labeling the nicks at the second sequence motif on the first DNA with a second label, wherein the third label is different from the second label; andrepairing the nicks on the first DNA following labeling with the second label.41. The method of claim 40 , wherein the first label and second label comprise the same label.42. The method of claim 37 , wherein the first DNA and the second DNA are each from the same source.43. The method of claim 37 , wherein the linearizing comprises transporting the DNA into a nanochannel.44. The method of claim 37 ...

METHODS OF MACROMOLECULAR ANALYSIS USING NANOCHANNEL ARRAYS

Methods of analyzing features such as the physical size of macromolecules or biomarkers along large genomic DNA molecules were disclosed as wen as the devices for carrying out such high throughput analysis in a massively parallel fashion. Methods of fabricating such devices are also disclosed. 1198-. (canceled)199. A method of detecting a copy number abnormality in a genome of interest , the method comprising:providing a first sample comprising isolated nucleic acid molecules of the genome of interest;labeling one or more regions of the nucleic acid molecules of the genome of interest;translocating at least a portion of at least a first region of the nucleic acid molecules of the genome of interest through a first fluidic nanochannel segment disposed substantially parallel to a first substrate surface, wherein the first fluidic nanochannel segment is capable of containing and elongating at least a portion of the region of the nucleic acid, and wherein the first fluidic nanochannel segment has a characteristic cross-sectional dimension of less than about 1000 nm and a length of at least about 10 nm;capturing signals corresponding to the first region of the genome of interest to ascertain a labeling pattern characteristic of the genome of interest;comparing the labeling pattern of the genome of interest with a corresponding labeling pattern of a control; andascertaining from the comparison a copy number of at least one of the regions of the nucleic acid molecules of the genome of interest.200. The method of claim 199 , wherein the control is a second region of the genome of interest.201. The method of claim 200 , further comprising ascertaining a copy number of the first region and the second region claim 200 , and then comparing the copy numbers of the first and second regions.202. The method of claim 201 , wherein the first region is a trisomy-linked region and the second region is different from the trisomy-linked region.203. The method of claim 202 , comprising ...

Item Eligibility and/or Promotion Determination Application

In general, the present invention is directed to systems and methods of utilizing an application operating on a mobile device to determine any applicable offers or eligibility and rank such offers and eligibility for user selection. More specifically, a process may be conducted by a processor in communication with a mobile device and data stores including user data, retailer data, and/or product and service data, the process including comparing identification information associated with a product or service received from the mobile device with data in the data stores; determining the product or service and identifying any associated promotional offers or plan eligibility associated with the product or service, the user, and/or the retailer; ranking any promotional offers or plan eligibility based at least in part on size of promotion; transmitting to the mobile device an identifier of applicable promotional offers or plan eligibility. 1. A system , comprising:a processor; user data, including memberships, groups, plans, and/or entitlements associated with a user;', products and services offered by a retailer;', 'any promotional offers offered by the retailer;, 'retailer data, including, 'product and service data, including identification information of each product and service, and any promotional offers or plan eligibility associated with the product or service;, 'one or more data stores, the one or more data stores storing data comprisingwherein the processor is in selective communication with one or more retailers, manufacturers, membership plans, insurance providers, and/or entitlement providers to populate and update the one or more data stores;wherein the processor is in selective communication with a mobile device of the user; comparing first media data comprising identification information associated with a product or service received from the mobile device with product and service data stored in the one or more data stores;', 'based at least in part on the ...

QUICK DISCONNECT CONNECTOR ASSEMBLY

A quick-disconnect connector assembly includes a housing having a bore that extends up to but not through a first end of the housing. The connector assembly also includes a proximity switch disposed within the bore, and the proximity switch includes a switch body, a first contact member, and a second contact member. A portion of each of the first and second contact members extends from the switch body towards a second end of the housing. In a first switch position, a contact of a displaceable switching assembly is in contact with the first contact member, and in a second switch position, the contact is in contact with the second contact member. The connector assembly also includes an external connection assembly including a first pin that is electrically coupled to the first contact member and a second pin that is electrically coupled to the second contact member. 1. A quick-disconnect connector assembly comprising:a housing that extends along a longitudinal axis from a first end to a longitudinally-opposite second end, the housing including one or more interior surfaces that cooperate to define a bore that extends from the second end to a point adjacent to the first end such that the bore does not extend through the first end of the housing, wherein the bore includes a first bore portion; a switch body extending along a body longitudinal axis, the switch body having a first end disposed adjacent to the first end of the housing and a longitudinally-opposite second end; and', 'a first contact member and a second contact member, each of the first and second contact members having a first end and a longitudinally-opposite second end, the second end being disposed within the switch body and the first end being disposed external to the switch body, wherein a portion of each of the first and second contact members extends from the second end of the switch body towards the second end of the housing,', 'wherein in a first switch position, a contact of a displaceable ...

Adjustable orthotic foot brace and method for adjusting a foot brace

The orthotic foot brace for a person wearing a footwear generally has: a lower leg holder securable to a lower leg of the person for use, a lower leg strut connected to the lower leg holder and extending downwardly towards the footwear during use; a foot strut structurally connected to the lower leg strut and having at least one side portion, each side portion extending forwardly along a respective side of the footwear and extending outwardly along a respective side of the footwear; an instep strut provided in the form of an extension of the foot strut along a long axis of the footwear, the instep strut having a distal portion securable to the footwear, and at least a proximal portion being slidably connected to the foot strut in a manner to allow adjusting the extension distance of the distal portion to a selected position.

TRACEABLE METALLIC PRODUCTS AND METALLIC SUPPORT FOR NANOSTORAGE

The invention relates to traceable metallic products, methods of uses and methods of making same. The metallic products may be made traceable for integrity purposes, identification purposes, counterfeit avoidance and the like. The invention also relates to metallic supports for nanostorage of various compounds and samples. 1. A traceable metallic product , wherein said metallic product comprises a porous surface layer formed by anodization , and wherein said porous surface layer comprises at least one traceable biological compound.2. (canceled)3. The traceable metallic product of claim 1 , wherein said at least one traceable biological compound is selected from the group consisting of nucleic acids claim 1 , peptidic molecules claim 1 , lipids claim 1 , mono and polysaccharides claim 1 , hormones claim 1 , vitamins claim 1 , and derivatives thereof.4. (canceled)5. The traceable metallic product of claim 1 , wherein said at least one traceable biological compound is carried in nanopores of the porous surface layer claim 1 , and wherein said at least one traceable compound is recoverable from said porous surface layer for detection claim 1 , identification and/or utilization purposes.6. (canceled)7. The traceable metallic product of claim 1 , wherein said traceable metallic product consists of a metallic support for nanostorage of compound and samples.8. The traceable metallic product of claim 1 , wherein said porous surface layer is sealed.9. The traceable metallic product of claim 1 , wherein said porous surface layer further comprises an electrodeposit of at least one metal selected from the group consisting of silver claim 1 , gold claim 1 , copper claim 1 , nickel claim 1 , zinc claim 1 , tin claim 1 , cadmium claim 1 , palladium and platinum.10. The traceable metallic product of claim 1 , wherein said traceable metallic product consists of a traceable piece of aluminum comprising a porous surface layer formed by anodization claim 1 , and wherein said porous ...

Photocleavage method and apparatus to clean fluidic devices

A method and system for improving throughput of a fluidic system such as a biopolymer analysis system by cleaning accumulated or clogging biopolymer from the fluidic system is disclosed. The method and system utilize a light energy source to photocleave the biopolymer molecules that may accumulate or aggregate in the fluidic system or clog a passageway. The accumulated biopolymer may be exposed to a light energy source for a sufficient period of time such that the biopolymer molecule is dosed with sufficient energy to photocleave the biopolymer molecules, thereby restoring the efficiency of and flow through the system.

NANONOZZLE DEVICE ARRAYS: THEIR PREPARATION AND USE FOR MACROMOLECULAR ANALYSIS

Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices. 1. A method for characterizing one or more features of a macromolecule , comprising:linearizing a macromolecule residing at least in part within a nanochannel,at least a portion of the nanochannel being capable of physically constraining at least a portion of the macromolecule so as to maintain in linear form that portion of the macromolecule, andthe nanochannel comprising at least one constriction;transporting at least a portion of the macromolecule within at least a portion of the nanochannel such that at least a portion of the macromolecule passes through the constriction;monitoring at least one signal arising in connection with the macromolecule passing through the constriction; andcorrelating the at least one signal to one or more features of the macromolecule.2. The method of claim 1 , wherein the macromolecule is selected from the group consisting of: a polynucleotide claim 1 , a polynucleoside claim 1 , a polymer claim 1 , a copolymer claim 1 , a dendrimer claim 1 , a surfactant claim 1 , a lipid claim 1 , a carbohydrate claim 1 , a polypeptide claim 1 , a protein claim 1 , and any combination thereof.3. The method of claim 1 , wherein the macromolecule resides within a fluid.4. The method of claim 1 , wherein the transporting comprises exposing the macromolecule to a gradient.5. The method of claim 4 , wherein the gradient is selected from the group consisting of: an electroosmotic field claim 4 , an electrophoretic field claim 4 , a magnetic field claim 4 , an electric field claim 4 , an electromagnetic field claim 4 , a flow field claim 4 , a radioactive field claim 4 , a mechanical force claim 4 , an electroosmotic force claim 4 , an electrophoretic force claim 4 , an electrokinetic force claim 4 , a temperature ...

VALVE SIGNATURE DIAGNOSIS AND LEAK TEST DEVICE

A valve signature diagnosis and leak testing device includes a spool valve operatively connected to a pilot valve, the pilot valve being configured to position the spool valve to one of an open position and a closed position. A blocker valve is fluidly connected to a control fluid outlet of the spool valve. An electrical module is operatively connected to the pilot valve, a supply of control fluid, and the blocker valve, the electrical module being capable of sending pulsed electrical signals to the pilot valve and the blocker valve to selectively position the spool valve and the blocker valve to an open or closed position.

Methods for single-molecule analysis

Methods for single-molecule preparation and analysis are disclosed herein. The methods can, for example, be used for isolating and analyzing DNA from various biological samples.

Embedded noble metal electrodes in microfluidics

A technique relates to manufacturing a nanogap. An oxide layer is disposed on top of a substrate. A release layer is disposed in a pattern on top of the oxide layer. A patterned trench is etched into the oxide layer using the pattern of the release layer. A metal layer is disposed on the release layer and in the patterned trench. A polish removes the release layer, thereby removing both the release layer and a portion of the metal layer having been disposed on top of the release layer, such that the metal layer remaining includes a first metal part and a second metal part connected by a metal nanowire. The metal layer remaining is coplanar with the oxide layer. A nanochannel is formed in the oxide layer in a region of the metal nanowire. The nanogap is formed in the metal nanowire separating the first and second metal parts.

Embedded noble metal electrodes in microfluidics

A technique relates to manufacturing a nanogap. An oxide layer is disposed on top of a substrate. A release layer is disposed in a pattern on top of the oxide layer. A patterned trench is etched into the oxide layer using the pattern of the release layer. A metal layer is disposed on the release layer and in the patterned trench. A polish removes the release layer, thereby removing both the release layer and a portion of the metal layer having been disposed on top of the release layer, such that the metal layer remaining includes a first metal part and a second metal part connected by a metal nanowire. The metal layer remaining is coplanar with the oxide layer. A nanochannel is formed in the oxide layer in a region of the metal nanowire. The nanogap is formed in the metal nanowire separating the first and second metal parts.

Compositions and Methods for siRNA Inhibition of Angiogenesis

System for cleaning and sanitizing

The present invention provides a system ( 10 ) for cleaning and sanitizing surfaces that incorporates a high pressure fluid supply ( 52 ) and a low pressure fluid supply ( 42 ), often carrying an ozonated fluid, and alternates directed streams of the two fluids for application to contaminated surfaces through a single applicator ( 70, 100 ).

COMPOSITION

Integrated analysis devices and related fabrication methods and analysis techniques

Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.

Cysteine engineered antibodies and conjugates withCysteine engineered antibodies and conjugates with a free cysteine amino acid in the heavy chain a free cysteine amino acid in the heavy chain

Disclosed is a cysteine engineered antibody compriDisclosed is a cysteine engineered antibody comprising a free cysteine amino acid having a thiol reasing a free cysteine amino acid having a thiol reactivity value in the range of 0.6 to 1.0; and a sectivity value in the range of 0.6 to 1.0; and a sequence in the heavy chain selected from SEQ ID NOSquence in the heavy chain selected from SEQ ID NOS: 11, 12, 13, and 15: LVTVCSASTKGPS SEQ ID NO:11 : 11, 12, 13, and 15: LVTVCSASTKGPS SEQ ID NO:11 LVTVSCASTKGPS SEQ ID NO:12 LVTVSSCSTKGPS SEQ ID LVTVSCASTKGPS SEQ ID NO:12 LVTVSSCSTKGPS SEQ ID NO:13 HTFPCVLQSSGLYS SEQIDNO:15 where the cysteiNO:13 HTFPCVLQSSGLYS SEQIDNO:15 where the cysteine in SEQ ID NOS: 11, 12, 13, and 15 is the free cne in SEQ ID NOS: 11, 12, 13, and 15 is the free cysteine amino acid. Also disclosed are antibody-drysteine amino acid. Also disclosed are antibody-drug conjugate compounds with the cysteine engineereug conjugate compounds with the cysteine engineered antibody described above and a drug moiety selecd antibody described above and a drug moiety selected from a maytansinoid, an auristatin, a dolastatted from a maytansinoid, an auristatin, a dolastatin, and a calicheamicin. in, and a calicheamicin.

Solenoid actuated butterfly valve

A butterfly valve (100) and a valve system using the butterfly valve are disclosed. The butterfly valve includes a valve body (102) and a valve disk (112) disposed in the body such that the valve disk may be rotated between at least a first position and a second position using a linear actuator. The actuator may be a linear solenoid coupled to the valve disk spaced a distance from the axis of rotation of the valve disk.

Multivalent antibodies and uses therefor. Multivalent antibodies and uses thereof.

Energy activated printing process

给出了反应性油墨和在基材上使用反应性且热激活的油墨产生图像的方法。在基材上印刷图像，而不使该油墨中的反应试剂反应。随后，使该反应试剂反应而将图像显著持久且牢固地固定到基材上。还将印刷的升华或类似热激活的染料印刷在该基材上。该升华或类似热激活的染料被激活，并且对施加到基材上的聚合物具有亲合性。

Method and apparatus for making insulating translucent panel assemblies

A method of making an insulating translucent panel assembly (10) such as a window or door lite is provided. In the method, a spacer (16) between two translucent panels (12) of glass or plastic includes a thermo- responsive sealing material (22). When heat and compression are applied to the spacer (16), the thermo-responsive sealing material (22) softens and fully seals the spacer (16) to the two translucent panels (12). A spacer (16) designed to be used in this method is also provided.

Patent DE4028660C2

Liquid flourescent dye concentrate for flow cytometry evaluation of virus-size particles and related products and methods

A kit, and method for flow cytometry include a liquid dye concentrate for fluorescent staining of virus-size particles with a plurality of fluorogenic dyes in a liquid medium. The liquid dye concentrate includes a plurality of fluorogenic dyes and one or both of (i) the liquid medium comprising a liquid mixture including water and liquid phase organic material and (ii) disaccharide dissolved in the liquid medium.

Labeling of dna

Methods of labeling DNA molecules comprising the use of one or more dCas proteins and one or more labeled guide RNAs (gRNAs) are described herein. In some embodiments, the labeled DNA molecules are elongated in fluidic nanochannels, where they can be analyzed.

Method of constructing and executing a process

Disclosed is a method of constructing and executing a process. A conventional process is minutely divided into minimum unit subprocesses, and the minutely divided subprocesses are classified into a decision subprocesses and a routine subprocess by whether they require decision-making. Any subprocess which is executable using the setup condition in a specific decision subprocess is classified into the routine subprocess in such a manner that the classified routine subprocess follows on the specific decision subprocess. One or a series of decision subprocesses are combined with one or a series of routine subprocesses which are executable on the condition of the completion of the decision subprocesses to form one unit process, and a job-support computer program is created to allow the plurality of subprocesses included in the one unit process to be successively executed. A plurality of subprocesses which are executable in accordance with common input data are detected from the minutely divided minimum unit subprocesses, and a job flow is constructed to allow the respective jobs in the plurality of subprocesses to be simultaneously initiated and executed in parallel. The present invention can drastically reduce the lead-time of a process while facilitating execution of the entire process with high efficiency.

Enclosed proximity switch assembly

An enclosed proximity switch assembly includes a top enclosure and a bottom enclosure that are coupled to form an interior volume. A shaft protrusion upwardly extends from a top surface of the top enclosure, and an interior bore portion having an enclosed volume is defined within the shaft protrusion to form a portion of the interior volume. A first end of a vertical shaft is rotatably disposed within the interior bore portion such that the shaft rotates relative to the top and bottom enclosures. A samarium cobalt target magnet is coupled to the shaft, and the target magnet interacts with a samarium cobalt driver magnet within a proximity switch when the target magnet is rotated within a predetermined distance of a top portion of the proximity switch. The interaction causes a switch to move from a first state to a second state, or vice versa.

Devices and methods for dynamic determination of sample position and orientation and dynamic repositioning

Provided are devices and methods for determining the spatial orientation of a target sample, which devices and methods are useful in auto focus systems. The devices and methods function by correlating (a) the location of radiation on a radiation detector of radiation reflected by the sample with (b) the position of the sample, and in some embodiments, adjusting the position of the sample, the position of an optical device, or both, in accordance with the location of radiation reflected by the sample onto the detector so as to maintain the sample in focus.

Component of printed circuit boards

A component (10) for use in manufacturing articles, such as printed circuit boards, comprising a laminate constructed of a sheet of copper foil (20) that, in a finished circuit board, constitutes a functional element, and a sheet of carbon steel (14) having a layer of an inert metal (16) thereon, the sheet of carbon steel (14) constituting a discardable element. One surface (22) of the copper sheet (20) and the surface (18) of the inert metal layer (16) on the carbon steel sheet (14) are essentially uncontaminated and engageable with each other at interfaces. The copper sheet (20) is attached to the inner metal layer (16) of the carbon steel (14) at their borders and defines substantially uncontaminated central zones inwardly of the edges of the sheets that are unjoined at the interfaces.

Data generating device, data generating method and data generating program

System and method for assessing properties of biomolecules

本发明涉及用于评估生物分子特性的系统和方法。具体地，本发明提供了用于评估多核苷酸上损伤的存在和程度的方法和系统。所述方法包括将标记物整合在损伤位点处并对所述标记物进行成像以确定所述损伤的存在和程度。所述系统包括能够在单个分子上执行损伤评估的装置。

Nanonozzle device arrays: their preparation and use for macromolecular analysis

Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices.

Systems and methods for providing, reloading, and redeeming stored value cards used in transit applications

The invention is directed to systems and methods of conducting transactions associated with a transit card. A method of conducting transactions may be conducted between a processor and a transit processor. Steps may include receiving a redemption request and determining if it is a pre-authorization request or a redemption; if the redemption request is a pre-authorization request, determining if the account is authorized for a particular transit type. If the account is authorized, communicating approval to the transit processor. If the account is not authorized communicating denial. If the redemption request is a redemption: determining if account value is sufficient to pay the redemption amount; if not, denying and if so approving the transaction and deducting the amount, and determining if the account value is below a pre-determined threshold for a particular transit types, and if so, updating the status of the account at a data store.

Labeling of dna

Methods of labeling DNA molecules comprising the use of one or more dCas proteins and one or more labeled guide RNAs (gRNAs) are described herein. In some embodiments, the labeled DNA molecules are elongated in fluidic nanochannels, where they can be analyzed.

Methods of macromolecular analysis using nanochannel arrays

Methods of analyzing features such as the physical size of macromolecules or biomarkers along large genomic DNA molecules were disclosed as well as t he devices for carrying out such high throughput analysis in a massively par allel fashion. Methods of fabricating such devices are also disclosed. The s ample embodiment shown in the figure illustrates detection of a macromolecul e flowing through a nanochannel device where passage of the macromolecule th rough the nanochannel is recorded by exciting features of interest to fluore sce with an excitation source, and then sensing the fluorescence with a phot on detection device, the resulting fluorescent signal being correlated along the length of the macromolecule.

Methods of macromolecular analysis using nanochannel arrays

Methods of analyzing features such as the physical size of macromolecules or biomarkers along large genomic DNA molecules were disclosed as well as the devices for carrying out such high throughput analysis in a massively parallel fashion. Methods of fabricating such devices are also disclosed.

Methods of macromolecular analysis using nanochannel arrays

Methods of analyzing features such as the physical size of macromolecules or biomarkers along large genomic DNA molecules were disclosed as well as the devices for carrying out such high throughput analysis in a massively parallel fashion. Methods of fabricating such devices are also disclosed.

Dual/variable gain oil pump control valve

A solenoid fluid control valve is disclosed for controlling a variable displacement pump. The solenoid fluid control valve comprises a fixed solenoid component (702), a movable armature component (710), a fixed nozzle body (722), a movable spool (720) within the fixed nozzle body, and a valve member (714). The valve member regulates fluid pressure in a first (746, 748) and second feedback chamber. Fluid in the second feedback chamber establishes a second feedback pressure that acts on the movable spool with a motive feedback force in a first axial direction. The movable spool moves in the first axial direction in response to the motive feedback force.

Integrated analysis devices and related fabrication methods and analysis techniques

Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided

Methods and devices for single-molecule whole genome analysis

Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g., determining structural variations and copy number variations between individuals.

A kind of industrial waste gas dust treatment device

本实用新型公开了一种工业废气粉尘处理装置，包括壳体，所述壳体内壁的顶部对称设置有第一支撑柱，两个第一支撑柱的底部通过第一吸尘管固定连接，所述壳体顶部的中点处固定连接有吸尘风机，所述吸尘风机的底部从上至下依次贯穿壳体和第一吸尘管且延伸至第一吸尘管的内部。本实用新型通过壳体、第一吸尘管、吸尘风机、过滤水箱、第二吸尘管、第三吸尘管、冲刷水箱、第一水管、冲刷喷头、旋转电机、旋转转轴、滚筒、海绵吸附棒、活性炭吸附层、过滤板、出水口、循环水池、循环水泵、第三水管和第四水管相互配合，起到了防逸散的效果，能够有效去除废气中的粉尘，保护了工作人员的身体健康，提高了工作人员的工作积极性。

For assessing the system and method for biomolecule characteristic

本发明提供了用于评估多核苷酸上损伤的存在和程度的方法和系统。所述方法包括将标记物整合在损伤位点处并对所述标记物进行成像以确定所述损伤的存在和程度。所述系统包括能够在单个分子上执行损伤评估的装置。

Photocleavage method and apparatus to clean fluidic devices

A method and system for improving throughput of a fluidic system such as a biopolymer analysis system by cleaning accumulated or clogging biopolymer from the fluidic system is disclosed. The method and system utilize a light energy source to photocleave the biopolymer molecules that may accumulate or aggregate in the fluidic system or clog a passageway. The accumulated biopolymer may be exposed to a light energy source for a sufficient period of time such that the biopolymer molecule is dosed with sufficient energy to photocleave the biopolymer molecules, thereby restoring the efficiency of and flow through the system.

Solenoid actuated butterfly valve

A butterfly valve (100) and a valve system using the butterfly valve are disclosed. The butterfly valve includes a valve body (102) and a valve disk (112) disposed in the body such that the valve disk may be rotated between at least a first position and a second position using a linear actuator. The actuator may be a linear solenoid coupled to the valve disk spaced a distance from the axis of rotation of the valve disk.

Trans-septal cardiac occluder device

本发明涉及一种可穿越间隔心脏封堵器装置(1)，包括：·‑支撑结构(2)；其中·‑所述支撑结构(2)包括界定内腔(4)的中心支撑结构部分(3)；·‑所述支撑结构(2)包括第一锚固部分(5)和相对的第二锚固部分(6)；·‑所述支撑结构(2)被配置成在用于通过患者的脉管系统插入的压缩管状配置与扩张或延伸配置之间扩张和收缩，在扩张或延伸配置中所述第一和第二锚固部分(5，6)从所述中心支撑结构部分(3)径向向外延伸以通过以下来压缩其间的间隔(7)：通过存在于所述间隔(7)中的缺损或孔或开口将所述支撑结构(2)布置成跨在所述间隔(7)上；并且其中·‑所述可穿越间隔心脏封堵器装置(1)进一步包括至少一个隔离件(8)；·‑所述隔离件(8)由所述支撑结构(2)支撑并且被布置成当所述隔离件(8)处于其中所述支撑结构(2)被延伸的松弛配置时闭合所述内腔(4)的大部分；·‑所述隔离件(8)被配置成允许插入到由所述间隔(7)界定的第一区室(9或10)中的医疗仪器穿过所述隔离件(8)，以及然后穿过所述内腔(4)进入由所述间隔(7)界定的第二区室(10或9)；其中·‑所述隔离件(8)包括多个长型膜封堵条状物或桥(21)或封堵桥(21)；·‑所述封堵桥(21)的每个包括长型本体(50)，所述长型本体具有相对的长型本体端部或端部(51，52)和相对的纵向边缘或边(22)；·‑在所述隔离件(8)的松弛配置中，所述多个封堵桥(21)中的每个封堵桥(21)被布置成接近于所述多个封堵桥(21)中的至少另一个封堵桥(21)；·‑每个封堵桥(21)的所述纵向边(22)的至少一部分和所述至少另一个封堵桥(21)的所述纵向边(22)的至少一部分界定在所述隔离件(8)上的至少一个狭缝或一个切口(27)；·‑每个封堵桥(21)的两个所述相对的端部(51，52)直接或间接地连接到所述支撑结构(2)上或由所述支撑结构(2)支撑。

Nanonozzle device arrays: their preparation and use for macromolecular analysis

Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices.

Methods for single-molecule analysis

Automated method for direct sampling of immune cells from whole blood or other biological samples in microwell plates

A method for automatic sampling of immune cells from a biological fluid sample, e.g., whole blood, deposited in a well of a microwell plate. The sample contains red blood cells (RBCs) and magnetic beads which are designed to bind to the RBCs. The microwell plate is placed on a shaker having a magnetic adapter including at least one magnet. The magnet causes the RBCs bound to the magnetic beads to be attracted to and migrate to a wall of the well (e.g., the bottom or side wall) and be held against the wall. The shaker is then operated to shake the microwell plate in a manner and for a time period so as to suspend substantially evenly or homogeneously the immune cells in the biological fluid sample within a region of the well but still retain the holding of the RBCs to the wall of the well such that the immune cells are substantially isolated from the RBCs in the region of the well. During or after shaking, a sample probe is then lowered into the region of the well to withdraw a portion of the sample containing the immune cells.

Passive reduction or elimination of frost and fog

Lens fogging or frosting on inside surfaces of an instrument lens may be reduced or prevented by sealing the instrument housing. Damage or rupture of the sealed housing due to an increase or decrease in pressure within the housing may be avoided while reducing introduction of moisture into the housing by venting the housing through a tube to a location having a relatively lower humidity.

Method of securing optical fiber components, devices and fibers to the same or to mounting fixtures

A method of bonding glass-based optical elements comprising the steps of positioning a first glass-based optical element relative to a second glass-based optical element, applying a glass-based bonding compound about the first and second optical elements, and applying sufficient localized heat to the glass-based bonding compound to cause the glass-based bonding compound to soften and fuse with the optical elements.

Systems and methods for authorizing use of validly sold merchandise

The invention is generally directed to a method of managing the activation and use of a specific merchandise, facilitated between at least a retailer that sells the specific merchandise, a customer that purchases the specific merchandise, and a central processor that authorizes activation and use of the specific merchandise. The method includes the steps of receiving an indicia that uniquely identifies the specific merchandise, and an activation code associated with the merchandise that will allow the merchandise to properly function, storing the indicia of the specific merchandise and the activation code, receiving a confirmation of a valid purchase transaction of the specific merchandise, the confirmation of valid sale comprising the indicia of the specific merchandise, determining the activation code associated with the specific merchandise, and providing the activation code associated with the specific merchandise to the customer.

Integrated analysis devices and related fabrication methods and analysis techniques

Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have re-duced background signals and that reduce quench-ing of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.

Proximity switch

Proximity switches are disclosed that preferably are provided in a hermetically sealed unit that can be used in harsh environments and under significant pressures, such as underwater and in nuclear power facilities, without having any parts that would require replacement or periodic maintenance. The proximity switches are preferably switches actuated by physical movement of a contact in response to changing magnetic forces. The switches are preferably disposed in a body tube optionally including a hermetic seal assembly to seal an open end of the body tube and/or a ferrule that prevents electrical wires attached to the switch inside the body tube from being pulled away from the switch. Further, the switches preferably maintain a contact pressure between electrical contacts sufficient to withstand acceleration seismic testing of 10g with no contact discontinuity.

Automatically define system behavior or user experience by recording, sharing, and processing information related to wide-angle images

QUICK DISCONNECT CONNECTOR ASSEMBLY

Un ensamble conector de desconexión rápida incluye una cubierta que tiene un hueco que se extiende hacia arriba pero no pasa a través de un primer extremo de la cubierta. El ensamble conector también incluye un interruptor de proximidad colocado dentro del hueco, y el interruptor de proximidad incluye un cuerpo del interruptor, un primer miembro de contacto y un segundo miembro de contacto. Una parte de cada uno del primer y segundo miembro de contacto se extiende desde el cuerpo del interruptor hasta un segundo extremo de la cubierta. En una primera posición del interruptor, un contacto de un ensamble de conmutación deslizable está en contacto con el primer miembro de contacto, y en una segunda posición del interruptor, el contacto está en contacto con el segundo miembro de contacto. El ensamble conector también incluye un ensamble de conexión externa que incluye una primera clavija que está acoplada eléctricamente al primer miembro de contacto y una segunda clavija que está acoplada eléctricamente al segundo miembro de contacto. A quick disconnect connector assembly includes a cover that has a recess that extends upward but does not pass through a first end of the cover. The connector assembly also includes a proximity switch placed inside the gap, and the proximity switch includes a switch body, a first contact member and a second contact member. A part of each of the first and second contact member extends from the switch body to a second end of the cover. In a first position of the switch, a contact of a sliding switch assembly is in contact with the first contact member, and in a second position of the switch, the contact is in contact with the second contact member. The connector assembly also includes an external connection assembly that includes a first pin that is electrically coupled to the first contact member and a second pin that is electrically coupled to the second contact member.

Die design support method and die design support system

The present invention provides a die design support method and system capable of reducing the time-period required for a die design, allowing an unskilled die engineer to perform a die design without difficulties, and coping with a product design change in a short time-period. A standard die design procedure is stored as “master die data” in advance using a function of a CAD system or the like. When a product design data is entered, the design procedure is automatically executed to the design data in accordance with the stored master die data. Preferably, the entire master die data is divided into a plurality of processes, such as a process of creating a scaling model, and a process of creating a parting model.

Circuit and method for independent control of series connected light emitting diodes

Described herein is a circuit and method for independent control of series connected light emitting diodes (LEDs). The circuit includes a first light emitting diode (LED) connected in series with a second LED. A current source is connected in series with the first LED and the second LED and a shunt circuit is connected in parallel with the first LED and the second LED. The shunt circuit includes a pair of serially connected resistors. The shunt circuit prevents inadvertent excitement of the LEDs due to leakage currents but minimally affect illumination characteristics of the LEDs. A pair of transistors is connected to the first LED and the second LED, respectively, and is biased using a set of bias resistors. A tri-state control signal switches on and off the pair of transistors and enables excitation of the first LED, the second LED or both via the current source.

systems and methods for assessing biomolecule characteristics

本发明涉及用于评估生物分子特性的系统和方法。具体地，本发明提供了用于评估多核苷酸上损伤的存在和程度的方法和系统。所述方法包括将标记物整合在损伤位点处并对所述标记物进行成像以确定所述损伤的存在和程度。所述系统包括能够在单个分子上执行损伤评估的装置。

Electronic-integration compatible photonic integrated circuit and method for fabricating electronic-integration compatible photonic integrated circuit

An electronic-integration compatible photonic integrated circuit (EIC-PIC) for achieving high-performance computing and signal processing is provided. The electronic-integration compatible photonic integrated circuit comprises a plurality of electronic circuit structures and a plurality of photonic circuit structures. The electronic and photonic circuit structures are integrated by a process referred to as monolithic integration. An electronic circuit structure includes one or more electronic devices and a photonic circuit structure includes one or more photonic devices. The integration steps of electronic and photonic devices are further inserted into standard CMOS process. The photonic circuit structures and the electronic circuit structures are integrated to form the electronic-integration compatible photonic integrated circuit device.

AUTOMATIC DEFINITION OF SYSTEM BEHAVIOR OR USER EXPERIENCE BY RECORDING, SHARING AND PROCESSING INFORMATION ASSOCIATED WITH WIDE ANGLE IMAGE

DEFINIÇÃO AUTOMÁTICA DE COMPORTAMENTO DO SISTEMA OU EXPERIÊNCIA DO USUÁRIO POR REGISTRO, COMPARTILHAMENTO E PROCESSAMENTO DE INFORMAÇÃO ASSOCIADA COM IMAGEM DE ÂNGULO AMPLO. Sistemas e métodos de acordo com a invenção permitem registro, compartilhamento e comunicação automáticos de parâmetros diferentes associados com imagens e seu gerador de imagem para definir um comportamento do sistema específico de um dispositivo de exibição, ou de uma unidade de algoritmo. Exemplos de informação incluem parâmetro dos geradores de imagem, parâmetros do ambiente, parâmetros de processamento e intensificação de imagem, coordenadas de uma seção de conteúdo de imagem de cena de ângulo amplo, parâmetros de exibição, experiência do usuário definida, comportamento do sistema definido, ou qualquer informação a ser registrada, compartilhada, e comunicada. Para evitar perda de informação, a informação é codificada diretamente na cena usando um marcador. Desse modo, a informação é robustamente transferida a partir do gerador de imagem para a unidade de exibição. De acordo com a informação, a imagem final pode ser automaticamente corrigida e intensificada antes da exibição, parâmetros associados diferentes podem ser exibidos na imagem final, ou serem usados com outra saída. A experiência final do usuário, ou comportamento do sistema, pode, desse modo, ser definido e ser reproduzido.

Methods and devices for single-molecule whole genome analysis

Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.

Die design support method and die design support system

The present invention provides a die design support method and system capable of reducing the time-period required for a die design, allowing an unskilled die engineer to perform a die design without difficulties, and coping with a product design change in a short time-period. A standard die design procedure is stored as "master die data" in advance using a function of a CAD system or the like. When a product design data (10) is entered, the design procedure is automatically executed to the design data in accordance with the stored master die data. Preferably, the entire master die data is divided into a plurality of processes, such as a process of creating a scaling model (12), and a process of creating a parting model (14).

A kind of flexible package labeling thermal transfer heating device

一种软包装贴签用热转印加热装置，包括定位块，所述定位块的上端设有第一电机，所述定位块内穿接有丝杆，所述丝杆的上端连接所述第一电机，所述丝杆的下端连接有加热环，所述加热环的轴线和所述丝杆垂直设置，所述加热环的下端设有缺口，所述缺口不大于所述加热环本体的二分之一；所述加热环内对应设有与所述加热环大小相适配的加热辊，所述加热辊与所述加热环之间留有间隙；所述加热辊的中央活动穿接设有固定管；所述加热环上均匀、固定穿接有若干电热棒，所述若干电热棒均连接有电源。本装置能提高防伪标签热转印效率，保证产品质量。

Automated definition of system behavior or user experience by recording, sharing, and processing information associated with wide-angle image

Systems and methods in accordance with the invention allow automatic recording, sharing, and communicating of different parameters associated with images and their imager to define a specific system behavior of a display device or an algorithm unit. Examples of information include imager parameters, environment parameters, image processing and enhancement parameters, display parameters, defined user experience, defined system behavior or the likes. To avoid loss of information, the information is encoded directly in the picture using a marker. This way, the information is robustly transferred from the imager to the display unit. According to the information, the final image can be automatically corrected and enhanced before display, different associated parameters can be displayed on final image or be used with another output. The end user experience or system behavior can thus be defined and be reproduced.

Methods and devices for single-molecule whole genome analysis

Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g., determining structural variations and copy number variations between individuals.

Software development support program, recording medium having the program stored thereon and software development support system

Respective items of a software component, a development task and a required specification, which are enumerated as the requirement for performing the development of software products belonging to a certain category are first registered in recording means. In the registration of the development-task items, when a specific development-task item can be initiated only after the completion of one or more other development-task items at the time of the registration, the specific development-task item is registered together with ordinal information specifying the one or more other development-task items. Then, the operator is allowed to set up the correlations between any two of the software component, the development task and the required specification which have been registered by the item registration function, with respect to each of the items thereof, and information about the correlations is registered in the recording means.

Extruded plastic window trim strip

Systems and methods for providing, reloading, and redeeming stored value cards used in transit applications.

La invención se relaciona con sistemas y métodos para llevar a cabo transacciones asociadas con una tarjeta de tránsito. Un método para llevar a cabo transacciones puede ser realizado entre un procesador y un procesador de tránsito. Las etapas pueden incluir recibir una solicitud de finiquito y determinar si existe una solicitud de preautorización o un finiquito; si la solicitud de finiquito es una solicitud de preautorización, determinar si la cuenta está autorizada para un tipo de tránsito particular. Si la cuenta está autorizada, comunicar aprobación al procesador de tránsito. Si la cuenta no está autorizada, comunicar prohibición. Si la solicitud de finiquito es un finiquito: determinar si el valor de la cuenta es suficiente para pagar la cantidad de finiquito; de no ser así, prohibir y si es así, aprobar la transacción y detectar la cantidad y determinar si el valor de cuenta está por debajo de un umbral predeterminado para un tipo de tránsito particular y, en caso de ser así, actualizar el estado de la cuenta en el almacenamiento de datos.

Delivery of value identifiers using short message service (SMS)

Extruded plastic window lift rail

Adjustable orthotic foot brace and method for obtaining a foot brace measurement

The orthotic foot brace for a person wearing a footwear generally has: a lower leg holder securable to a lower leg of the person for use, a lower leg strut connected to the lower leg holder and extending downwardly towards the footwear during use; a foot strut structurally connected to the lower leg strut and having at least one side portion, each side portion extending forwardly along a respective side of the footwear and extending outwardly along a respective side of the footwear; an instep strut provided in the form of an extension of the foot strut along a long axis of the footwear, the instep strut having a distal portion securable to an instep portion of the footwear, and at least a proximal portion being slidably connected to the foot strut in a manner to allow adjusting the extension distance of the distal portion to a selected position.

Systems and methods for delivering content over a network

A content delivery system that uses a graphical user interface to introduce users to and allow them to select from available content. A game delivery system that uses game players, such as emulators, to execute software written to run on a plurality of game platforms. The systems include a scalable, dynamic interface that launches and manages game players in a manner that is largely transparent to the user, and a combination of linear and on-demand content provides users with a managed gaming experience not unlike that of interactive television. In addition, the system includes a graphical user interface for allowing a primary user of an account to set user-specific controls for one or more users listed on the account.

Ankle foot orthosis

The ankle foot orthosis has a foot section, a leg section having a leg engagement portion extending in front of a user's lower leg and being securable against a shin area of the user's lower leg, and two arm portions extending downwardly from the leg engagement portion, on opposite sides of the user's lower leg, and a hinge section having two hinge members disposed on opposite sides of the user's ankle, each hinge member having two ends being elastically stretchable away from one another, with a first one of the ends being secured to a corresponding one of the leg arm portions, and a second one of the two ends being secured to the foot section, with a spacing between the hinge members and the arm portions forming a foot engagement path allowing the user to respectively engage or disengage his foot from the footwear across the spacing.

Methods for single-molecule analysis

本发明公开了单分子制备和分析方法。该方法例如可用于从各种生物样品中分离和分析DNA。

Methods of macromolecular analysis using nanochannel arrays

Methods and devices for single-molecule whole genome analysis

Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.

A method of manufacturing a data card assembly

Curved grating spectrometer and wavelength multiplexer or demultiplexer with very high wavelength resolution

The present application discloses a system comprising a compact curved grating (CCG) and its associated compact curved grating spectrometer (CCGS) or compact curved grating wavelength multiplexer/demultiplexer (WMDM) module and a method for making the same. The system is capable of achieving a very small (resolution vs. size) RS factor. The location of the entrance slit and detector can be adjusted in order to have the best performance for a particular design goal. The initial groove spacing is calculated using a prescribed formula dependent on operation wavelength. The location of the grooves is calculated based on two conditions. The first one being that the path-difference between adjacent grooves should be an integral multiple of the wavelength in the medium to achieve aberration-free grating focusing at the detector or a first anchor output slit even with large beam diffraction angle from the entrance slit or input slit, the second one being specific for a particular design goal of a curved-grating spectrometer.

A kind of communication receiver facilitating installation

本实用新型提供一种方便安装的通信用接收器，涉及通信技术领域。该方便安装的通信用接收器，包括墙体和通信用接收器本体，所述墙体位于通信用接收器本体的左方，墙体的右侧面设置有定位板，墙体的内左壁开设有第一小孔，定位板的左侧面上下均固定连接有插条，插条远离定位板的一侧延伸到第一小孔的内部，定位板的右侧面分别固定连接有第一横板和第二横板。该方便安装的通信用接收器，通过在空腔内设置弹簧配合限位柱，同时利用拉杆和拉块，使通信用接收器方便安装，有效的解决了通信用接收器用来接收信号并进行储存，而当前的通信用接收器不方便安装在墙上，延长了安装工人安装所需要的时间，间接的提高了安装所需要花费费用的问题。

Reduction of bias in genomic coverage measurements

Methods are provided for detecting and quantitating molecules using fluidics. In some embodiments, the methods comprise minimizing or eliminating biases caused by label density, or minimizing or eliminated biases caused by factors other than label density. In some embodiments, the methods comprise automated identification of genetic structural variation. In some embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor.

Artificial paver unit

The design consists of the features of shape, ornamentation and configuration of the artificial paver unit shown in the drawings.

Feed addictive, its preparation method and include its feed

本发明提供了一种饲料添加剂。该饲料添加剂包含如下重量份数的组分：20～40份的茶多酚、10～20份的维生素E、10～20份的维生素C、20～40份的白炭黑和6～12份的抗氧化剂混合物，所述抗氧化剂混合物为按重量比1:1:1混合的乙氧基喹啉、二丁基羟基甲苯和丁基羟基茴香醚。本发明饲料添加剂在消除动物体内过量自由基，缓解动物生长应激的作用功效的基础上强化产品对饲料抗氧化保护效果，在不需要额外使用其它防腐剂产品的条件下，按推荐使用剂量单一使用本产品，起到饲料抗氧化、防腐的效果，有效延缓饲料营养物质发生酸败反应，保证饲料营养价值及延长饲料保质期。让使用者在使用一种产品即可达到双重功效，提高产品的性价比。

Configurable process control device with electronic display assembly

A configurable process control device (10), which includes a field device(12), such as a valve position controller, that can be configured by a user to emulate any one of a plurality of different types of process control devices, is provided with an electronic display assembly (16). The electronic display assembly is operatively connected with a control circuit (18) that is arranged to respond to the specific configuration of the field device to cause the electronic display assembly to display information relevant to the specific type of control device the field device has been configured to emulate. The information may include safety certification information specific to each of the different types of process control devices that the field device can be configured to emulate.

Method of constructing and executing process

Disclosed is a method of constructing and executing a process. A conventional process is minutely divided into minimum unit subprocesses, and the minutely divided subprocesses are classified into a decision subprocesses and a routine subprocess by whether they require decision-making. Any subprocess which is executable using the setup condition in a specific decision subprocess is classified into the routine subprocess in such a manner that the classified routine subprocess follows on the specific decision subprocess. One or a series of decision subprocesses are combined with one or a series of routine subprocesses which are executable on the condition of the completion of the decision subprocesses to form one unit process, and a job-support computer program is created to allow the plurality of subprocesses included in the one unit process to be successively executed. A plurality of subprocesses which are executable in accordance with common input data are detected from the minutely divided minimum unit subprocesses, and a job flow is constructed to allow the respective jobs in the plurality of subprocesses to be simultaneously initiated and executed in parallel. The present invention can drastically reduce the lead-time of a process while facilitating execution of the entire process with high efficiency.

proximity switch

1/1 resumo interruptor de proximidade interruptores de proximidade são divulgados que preferivelmente são fornecidos em uma unidade hermeticamente selada que pode ser usada em ambientes adversos e sob pressões significativas, tais como debaixo d'água e em instalações de energia nuclear, sem que nenhuma peça exija substituição ou manutenção periódica. interruptores de proximidade são de preferência acionados por movimento físico de um contato em resposta às mudanças de forças magnéticas. os interruptores são dispostos de preferência em um tubo de corpo, opcionalmente, incluindo um conjunto de vedação hermético para vedar uma extremidade aberta do tubo de corpo e/ou uma virola que impede que os fios elétricos anexados ao interruptor no interior do tubo de corpo sejam puxados para longe do interruptor. além disso, os interruptores de preferência mantêm uma pressão de contato entre os contatos elétricos suficiente para suportar testes sísmicos de aceleração de 10 g sem descontinuidade de contato. 1/1 summary Proximity Switch Proximity switches are disclosed that they are preferably supplied in a hermetically sealed unit that can be used in harsh environments and under significant pressures, such as underwater and nuclear power installations, without any parts requires replacement or periodic maintenance. Proximity switches are preferably actuated by physical movement of a contact in response to changes in magnetic forces. the switches are preferably arranged in a body tube, optionally including an airtight seal assembly to seal an open end of the body tube and / or a ferrule that prevents the electrical wires attached to the switch inside the body tube from being pulled away from the switch. In addition, the switches preferably maintain a contact pressure between the electrical contacts sufficient to withstand 10 g acceleration seismic tests without contact discontinuity.

Energy management system for motor-assisted user-propelled vehicles

An energy management system for a motor-assisted user-propelled vehicle comprising a motor capable of assisting in propelling the vehicle and a rechargeable power supply for supplying energy to the motor. The system comprises: a user input for providing a desired value for each of at least one control parameter related to the user; a sensor for each control parameter for obtaining an actual value of the control parameter; a value comparator for receiving the desired value and the actual value of each control parameter and comparing them to generate a comparison signal for each control parameter; a command generator for generating a motor command using at least one comparison signal; and a motor controller for operating the motor, using the motor command, either to assist in propelling the vehicle, or act to recharge the power supply, in a way to bring the actual value closer to the desired value.

Polynucleotide mapping and sequencing

The present invention provides methods of obtaining structural information about a biopolymer sample. The meth-ods include labeling portions of a biopolymer, such as DNA or RNA, linearizing the biopolymer in some cases, and determining the distance between the labels. The user can then compare different samples' between-label distances to qualitatively compare different samples and to assay a given sample for additions or deletions of nucleotides in the regions flanked by the labels. The methods also permit sequencing of biopolymers.