НОВЫЙ СПОСОБ ПРОИЗВОДСТВА МЕТАНОЛА ИЗ НИЗКОКАЧЕСТВЕННОГО СИНТЕТИЧЕСКОГО ГАЗА

Настоящее изобретение относится к способу производства метанола из синтетического газа, а также к компоновке реактора для его осуществления. Способ включает следующие стадии: - предоставление свежего сжатого газа для синтеза метанола, содержащего водород, монооксид углерода и диоксид углерода, который предварительно нагревают и пропускают через адиабатический метанольный предварительный конвертер/предохранительный реактор, в котором синтетический газ частично конвертируют в метанол в присутствии гетерогенного метанольного катализатора, а ядовитые примеси из подаваемого газа удаляют, - предоставление рециркуляционного газового потока из схемы синтеза, содержащего частично конвертированный газ для синтеза метанола, и смешивание части рециркуляционного газового потока со свежим синтетическим газом, если это необходимо, как в случае частичной загрузки, с получением потока технологического газа, - охлаждение потока технологического газа из предварительного конвертера/предохранительного реактора ...

СПОСОБ И УСТРОЙСТВО ДЛЯ ИЗГОТОВЛЕНИЯ БИОПОЛИМЕРНЫХ МАТРИЦ

Группа изобретений относится к суперпараллельной аналитике биополимеров с применением микрополимерных полей. Способ изготовления биополимерных матриц осуществляют путем микродозирования на поверхность предметного носителя минимальных количеств жидкости с помощью аппликатора с капиллярным пространством, приводным капилляром и пипеточным кончиком. Причем в приводной капилляр подают буферный раствор из питающей емкости с возможностью перемещения кончика капилляра в трехмерном пространстве. При приложении к приводному капилляру электрического напряжения соответствующей полярности обеспечивается всасывание дозируемого биополимера или нанесение его на поверхность предметного носителя. Представлено также устройство для изготовления биополимерных матриц. Достигается простота и надежность нанесения минимальных количеств жидкости на биополимерную матрицу. 2 н. и 9 з.п. ф-лы, 1 ил.

АМПЛИФИКАЦИЯ БИБЛИОТЕК НУКЛЕИНОВЫХ КИСЛОТ С ПРИМЕНЕНИЕМ КИНЕТИЧЕСКОГО ИСКЛЮЧЕНИЯ

КАТАЛИТИЧЕСКАЯ СИСТЕМА ИНТЕЛЛЕКТУАЛЬНОЙ КОМБИНАТОРНОЙ ОПЕРАНДО СПЕКТРОСКОПИИ

... 1. Аппарат для разработки материала, содержащий комбинаторную реакторную матрицу, имеющую камеры, причем в каждой соответствующей камере протекает некая реакция; первый прибор, выполненный с возможностью измерения объемных и поверхностных структур и поверхностных частиц во время каждой соответствующей реакции; и второй прибор, выполненный с возможностью измерения продуктов реакции из каждой камеры во время каждой соответствующей реакции. 2. Аппарат по п.1, в котором реакция представляет собой каталитическую реакцию. 3. Аппарат по п.1, в котором каждый из первого и второго приборов выполняет свои соответствующие измерения в виде временной последовательности измеряемых образцов во время каждой соответствующей реакции. 4. Аппарат по п.2, в котором первый прибор включает в себя два устройства, каждое из которых использует отличающуюся методику для измерения объемных и поверхностных структур катализатора для данного одного из катализаторов. 5. Аппарат по п.3, в котором только одно из этих двух ...

Automatisierter Polypeptidsyntheseapparat.

Vorrichtung zur Zufuhr von Eduktflüssigkeiten

Die erfindungsgemäße Vorrichtung dient der gleichzeitigen Zufuhr von schwerflüchtiger Eduktflüssigkeit zu einer Pluralität von Mischpunkten beziehungsweise zu einer Pluralität von Reaktoren, wobei die Vorrichtung einen Vorratsbehälter, eine Zufuhrleitung und einen Splitter umfasst, durch den die Zufuhrleitung in eine Gruppe von nachgeordneten Leitungen aufgeteilt wird. Jede einzelne nachgeordnete Leitung steht mit je einem Mischpunkt oder je einem Reaktor in Wirkverbindung und ist mit jeweils einem Restriktorelement ausgestattet, wobei die Restriktorelemente und zumindest Teile der nachgeordneten Leitungen mit einer Ummantelung in Kontakt sind, die eine Temperierungseinheit aufweist.

Mikroreaktorsystem zum Erzeugen und Testen von Substanzen und Wirkstoffen

The invention relates to a microreactor system for producing and testing substances and active ingredients, whereby the system comprises separate microreactors which are arranged in an area that is configured in the shape of a matrix with n rows and m columns consisting of microreactors. Each microreactor has a sample chamber for accommodating substances and active ingredients, and a first and second feed are assigned to each sample chamber. Each microreactor also has at least one discharge leading out of the sample chamber in order to empty the same, whereby the respective first feeds of the microreactors of each of the n rows are interconnected via a respective first feed connection channel. In addition, the respective second feeds of the microreactors of each of the m columns are interconnected via a respective second feed connection channel. The system is characterized in that the respective first and second feeds lead into a mixing section which is provided in the form of a channel ...

Reaktorsteuerungssystem

Vorrichtung und Verfahren zur parallelen Durchführung von Experimenten

Es wird eine modulare Vorrichtung und ein Verfahren zur parallelen Durchführung von Experimenten an Prozessstoffen für die Entwicklung von technischen Verfahren beschrieben. Die modulare Vorrichtung eignet sich insbesondere zur miniaturisierten, automatisierten Durchführung komplexer Verfahren. Die Vorrichtung besteht wenigstens aus einer Vielzahl von Einzelreaktoren (1) zur Aufnahme der Prozessstoffe (10), wobei die Einzelreaktoren (1) unabhängig voneinander wenigstens bezüglich ihres inneren Drucks und ihrer Temperatur und der Drehzahl steuerbar oder regelbar sind und wobei die Einzelreaktoren (1) mindestens aufgebaut sind aus: DOLLAR A je einer druckfesten Kammer (2, 2a, 2b, 2c, 2d, 2e) mit einem separierbaren Probengefäß (12), gegebenenfalls je einer Rührvorrichtung (4), DOLLAR A je einer Heizeinrichtung (31) und/oder einer Kühleinrichtung (32) zur Temperierung des Probengefäßes (12), DOLLAR A einer Kontrolleinheit (700) zur Kontrolle oder Regelung wenigstens des Drucks und der Temperatur ...

VACUUM-OPERATED VALVE ASSEMBLY

... 1307087 Valves BECKMAN INSTRUMENTS Inc 31 March 1970 [8 April 1969] 36979/71 Heading F2V A valve assembly for use in automatic analysis (see Divisions B1-B2) comprises two identical independent valve units 103, 105 mounted on a common manifold 106. Valve unit 105 comprises manifold 106 as valve body, fluid recess 107 formed in the body, flexible diaphragm 109 extending over the recess, fluid inlet passage 100, 128 communicating with the recess, fluid outlet passage 130, 102 closed from the recess when valve poppet 125 is in valve seat on 135, housing 111 secured to body 106, a poppet guide comprising disc 117 and shaft 118 slidable in bore 114 in the housing with a centre portion of diaphragm 109 tightly sandwiched between poppet 125 and disc 117 and yieldably acting means in the housing, for example spring 120 retained between cap 115 and screw fitting 123 in poppet 125 for yieldably urging the poppet into engagement with valve seat 135 and a vacuum passage 126, 104 in the housing, whereby ...

Apparatus for and method of synthesizing biopolymer and method of recovering reagent for synthesizing biopolymer

Process

REACTION SYSTEM FOR SEQUENTIALLY TRANSFERRING REAGENTS FROM A PLURALITY OF STORAGE VESSELS

... 1,235,726. Dispensing measured quantities of liquids. DANISH INSTITUTE OF PROTEIN CHEMISTRY. 17 Sept., 1968 [18 Sept., 1967], No. 44160/68. Heading B8N. [Also in Divisions B1-B2] A reaction system for use for example in the solid state synthesis of peptides comprises a plurality of first vessels 111a-111c for reagents, a second vessel 112 which may be a metering vessel, a plurality of conduits 115a- 115c free of valves and each connecting one first vessel to the second vessel, and an automatic control system for controlling the flow of reagents from the first vessels to the second vessel by sequentially selectively applying to the reagents in the first vessels 111a &c. by means of gas pressure in lines 122, 116a &c., a first pressure which is higher than the pressure in the second vessel 112 so that reagent flows into the second vessel, and a second pressure which is lower than the pressure in the second vessel so that reagent ceases to flow to the second vessel and flows back from the ...

SYNTHESIS MECHANISM FOR OLIGONUKLEOTIDE UNDER USE OF CENTRIFUGAL FORCE

DEVICE FOR THE MONITORING OF THE RIVER RATE

PROCEDURE AND ARRANGEMENT FOR THE SIMULTANEOUS EVALUATION OF SEVERAL CATALYSTS

PROCEDURE FOR REMOVING FROM TRITYL SCHUTZGRUPPEN FROM 5 ' - O-TRITYL-NUCLEOSIDES AND OLIGONUKLEOTIDEN.

PROCEDURE AND DEVICE FOR THE MICRO DOSAGE OF SMALLEST ONES LIQUID QUANTITIES FOR BIO POLYMER ARRAYS

Method and device for microdosing the smallest amounts of liquid for biopolymer arrays

Oligonucleotide synthesizer

Chemical processing microsystems, diffusion-mixed microreactors and methods for preparing and using same

APPARATUS FOR AUTOMATICALLY PERFORMING CHEMICAL PROCESSES

AUTOMATIC SYNTHESIZER FOR DNA OR THE LIKE

A DEVICE FOR THE ADDITION OF A COMPOUND OR COMPOUND MIXTURE TO ANOTHER UNDER VARIOUS REACTION CONDITIONS, PARALLEL OR OTHERWISE

A device for the addition of a compound or compound mixture to another under various reaction conditions, parallel or otherwise is disclosed. The method and apparatus offer significant advantages over conventional experimental reactors, and is especially useful for synthesizing and screening large numbers of compounds. The device generally consists of a body (10) containing one or more recessed areas (14) surrounding one or more hollow pistons (18) mounted in barrels (30) covered by a top-plate (24). The recessed areas are filled with compounds. The hollow pistons are filled with other compounds. Using gas pressure the pistons move. This movement results in the addition of the compounds contained in the recessed areas to the compounds contained in the pistons. Modifications as found for conventional experimental reactors can also be incorporated in the apparatus.

DEVICE AND METHOD FOR CARRYING OUT EXPERIMENTS IN PARALLEL

Modular device and method for carrying out experiments in parallel on process substances to develop technical methods, comprising at least a multiplicity of individual reactors which are controllable independently of one another said reactors being comprised of pressure-tight chambers having separable sample vessels, and optionally, stirring devices, heating instruments, cooling instruments, or both, a monitoring unit for monitoring or controlling at least the pressure and the temperature in the individual reactors, pressure-tight lids having independent feed lines and optionally independent discharge lines for individual process substances; a plurality of the lids being simultaneously sealable upon the rectors by a common sealing means (11).

POLYMER SYNTHESIZER

The present invention provides a polymer synthesizer having a high efficiency pro-duction rate. The synthesis of the polymers, and more particularly of DNA and RNA, is done very quickly. Furthermore, it is possible to synthesize a plurality of polymers in the same batch without significantly increasing the time and the complex-ity of the process.

APPARATUS AND METHOD FOR THE SEQUENTIAL PERFORMANCE OF CHEMICAL PROCESSES

... 2026205 9009595 PCTABS00001 A compact apparatus (11) for the N-terminal and C-terminal sequencing of proteins and peptides is disclosed. The apparatus comprises a heated coupling and reaction chamber (RC) to accommodate a reaction cartridge such as a CFR containing a peptide sample (S) for sequential reaction with a plurality of reagents (R1, R2, and R3), and solvents (S1 and S2). A multi-inlet valve (VMI) controls flow of the reagents and solvents to the reaction chamber, and a transfer valve (VT) controls flow of fluids from the reaction chamber either to waste or to a conversion flask confined within a heated conversion chamber (CC). The conversion products are then conveyed through a selector valve (VS) and a fluid detection means (FD) to an analytical system. The multi-inlet valve, reaction chamber, transfer valve, conversion chamber and selector valve are all supported on a frame (11) comprising a horizontal base (12), upright support (13) and horizontal cover (14) secured at one ...

Vorrichtung zur Durchführung von mehrstufigen Reaktionen

Multiple bioreactor device.

СПОСОБ ОЦЕНКИ ЗАГРЯЗНЕНИЯ, ВЫЗЫВАЕМОГО НЕФТЕЗАВОДСКИМ СЫРЬЕМ

В заявке описан способ оценки воздействия загрязнения, вызванного нефтезаводским сырьем, на один или несколько процессов нефтепереработки, при осуществлении которого (i) подготавливают нефтезаводское сырье множества типов и/или множество фракций нефтезаводского сырья одного или нескольких типов, (ii) создают массив, включающий множество образцов металлов, используемых в металлических частях нефтезаводского оборудования, (iii) вводят каждый из образцов металла в контакт с упомянутым нефтезаводским сырьем или фракциями нефтезаводского сырья одного или нескольких типов при нестатических условиях и (iv) определяют степень загрязнения нефтезаводского сырья и/или его фракции. Образцы металлов предпочтительно используют в форме изготовленного средствами микротехнологии массива и путем высокопроизводительного экспериментирования осуществляют их параллельное испытание.

Catalytic process exploitation apparatus and method

A catalytic procedure development apparatus is disclosed, comprising a fresh reactant source, a control valve, a sampling valve, a temperature control device, a first reactor with high transferring rate and a second reactor with low transferring rate; performances at different longitudinal positions of a catalyst bed of a composite plug flow reactor can be simulated by inputting the fresh reactants and ejections of the first reaction in diverse proportions to the second reactor; and the influence to reaction course by these substances can also be tested by inputting other substances into the second reactor, such as reaction products, byproducts and pollutants and toxic substances which are likely present in industrial reactor raw materials. Dynamics, quality transfer and heat transfer performances of the composite plug flow reactor can be tested by sampling and analyzing ejections of the first reactor and the second reactor. In addition, the invention also relates to a method for development ...

Method and system for analysis of high throughput

Method and apparatus for rapid screening of multiphase reactions

Biological micro-reactor suitable for high throughput screening, forms alternating mobile liquid reactors and liquid separators in parallel capillaries

L'invention concerne un dispositif microfluidique d'injection de trains de chambres mobiles de réaction (102, 103) avec séparateurs (101) non miscibles dans des micro-canaux (21 à 26), comprenant : - des moyens d'injection (10) pour injecter en alternance et en parallèle dans les micro-canaux du liquide pour former les chambres mobiles de réaction et du liquide pour former les séparateurs, - des moyens de contrôle de la progression de l'un des deux liquides, disposés pour intervenir sur une zone (31) de chaque micro-canal délimitant un volume a injecter de ce liquide, les moyens de contrôle étant aptes à provoquer l'arrêt ou le ralentissement de la progression de ce liquide sur la zone de chaque micro-canal en exerçant une action basée sur une propriété physico-chimique de ce liquide, cette action n'ayant pas d'effet direct sur l'autre liquide.

DEVICE OF GRAFTING MICROSTRUCTURE OF PROTEINS ON A SUBSTRATE

Dispositif pour le greffage micro-structuré de plusieurs protéines sur un substrat, qui comprend un substrat(7), une couche, une matrice(10), une source d'une lumière(9), un système optique(11), une première solution aqueuse(1), une seconde solution aqueuse(2) et un circuit micro-fluidique, dans lequel la couche est disposée sur le substrat, la source éclaire la matrice par la lumière, la matrice propage la lumière selon un premier motif structuré, la matrice comprend des moyens optiques de remplacement du premier motif structuré par un second motif structuré, le système optique forme, sur la couche, une première image microstructurée du premier motif, le circuit contient la première solution aqueuse, le circuit comprend une ouverture pour la première solution, la première solution est en contact avec la couche au niveau de l'ouverture, le circuit comprend des moyens micro-fluidiques de remplacement de la première solution par la seconde solution, la couche comprend un polyéthylène glycol ...

Micro-fluidic heating system

본 발명은 미소유체 가열 시스템에 관한 것으로, 챔버, 유로 및 밸브를 제공하는 미소유체제어소자 및 이와 접촉되어 챔버의 내부를 가열시키는 모체로 구성된다. 상기 미소유체제어소자는 챔버, 유로 및 밸브가 형성된 상부기판과, 상부기판에 접합된 박막 형태의 하부기판으로 구성되고, 상기 모체는 가열수단이 형성된 멤브레인과 멤브레인을 지지하는 구조체로 구성된다. 상기 가열수단이 챔버 부분의 하부기판과 국부적으로 접촉되어 챔버의 내부를 가열시키기 때문에 열전달 효율이 극대화되며, 어레이 형태의 챔버를 갖는 구조의 경우 각 챔버의 온도를 독립적으로 제어할 수 있다. The present invention relates to a microfluidic heating system, comprising a microfluidic control element for providing a chamber, a flow path and a valve, and a mother body in contact with it to heat the interior of the chamber. The microfluidic control device includes an upper substrate on which a chamber, a flow path and a valve are formed, and a lower substrate in the form of a thin film bonded to the upper substrate, and the matrix includes a membrane on which a heating means is formed and a structure supporting the membrane. Since the heating means is in contact with the lower substrate of the chamber portion to heat the interior of the chamber, the heat transfer efficiency is maximized, and in the case of the structure having an array-type chamber, the temperature of each chamber can be independently controlled. 미소유체제어소자, 모체, 마이크로 히터, 챔버, 멤브레인 Microfluidic Control Element, Matrix, Micro Heater, Chamber, Membrane

HIGH THROUGHPUT MECHANICAL ALLOYING AND SCREENING

The invention provides an apparatus and methods for conducting ball milling operations in a high throughput manner. Also provided are devices and methods for high throughput screening of alloys and other materials to identify those that have desired gas storage properties.

DEVICE AND METHOD FOR PRESSURE AND FLOW CONTROL IN PARALLEL REACTORS

The present invention relates to a method and a device for the parallel study of chemical reactions in at least two specially separated reaction spaces. In particular, the invention is suitable for reactions which are not constant volume reactions and/or for reactions in which fluid flows through at least two spatially separated reaction spaces are intended to be controlled together for all the reaction spaces, or for related subsets of them, in the most straightforward way possible. According to one embodiment, the device according to the invention for the parallel study of chemical reactions comprises at least the following components: (a) at least two spatially separated reaction spaces (20); (b) on the reaction space input side, at least one common educt feed (10) for the reaction spaces according to (a); (d) on the reaction space output side, at least one connection (54) per reaction space to at least one holding gas feed (52) common to all the reaction spaces, or subsets of them; ...

Multi-reactor synthesizer and method for combinatorial chemistry

Solid phase combinatorial synthesis is carried out in a multi-reactor synthesizer comprising an array of separate reactor cells containing a solid reaction support medium. Subsets of reactor cells are interconnected with one another in a desired pattern, and, in a first reaction step, reagents are circulated through the subsets. After the first reaction step is completed, the reactor cells are rearranged into a new configuration of subsets, and the same, or other, reagents are circulated through the new subsets. The process can be repeated to produce large numbers of compounds differing from one another.

Multi-temperature modular reactor and method of using same

An apparatus and method for carrying out and monitoring the progress and properties of multiple reactions is disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight.

Combinatorial Electrochemical Synthesis

... Abstract of Disclosure An array of selectively addressible microelectrodes for combinatorial synthesis of complex polymers or alloys.

Method of searching for and generating polymrophs of a substance

The present invention provides a method and apparatus for producing polymorphs of a desired substance providing a plurality of enclosed spaces. The temperature and pressure of the enclosed spaces can be controlled. Various crystal identification means can be used to identify the polymorphic form of the solidified substance.

PROCESS AND APPARATUS FOR TRANSFERRING AIR-SENSITIVE SUBSTANCES

УСТРОЙСТВО ДЛЯ МИКРОСТРУКТУРИРОВАННОЙ ПРИВИВКИ БЕЛКОВ НА ПОДЛОЖКЕ

Изобретение относится к области микроструктурированной прививки белков на подложке и способу микроструктурированной прививки белков, использующему такое устройство. Устройство содержит подложку, пленку, содержащую полиэтиленгликоль и расположенную на подложке, матрицу для распространения света в соответствии с первым структурированным рисунком и для замены первого структурированного рисунка вторым структурированным рисунком, источник света для освещения матрицы, оптическую систему для формирования на пленке первого микроструктурированного изображения первого структурированного рисунка, первый резервуар для вмещения первого водного раствора, содержащего первое средство прививки и первый белок, второй резервуар для вмещения второго водного раствора, содержащего второе средство прививки и второй белок, и микрожидкостный контур для содержания первого водного раствора, содержащий отверстие для приведения в контакт первого водного раствора с пленкой у отверстия. При этом микрожидкостный контур ...

АМПЛИФИКАЦИЯ БИБЛИОТЕК НУКЛЕИНОВЫХ КИСЛОТ С ПРИМЕНЕНИЕМ КИНЕТИЧЕСКОГО ИСКЛЮЧЕНИЯ

FIELD: biotechnology.SUBSTANCE: group of inventions relates to methods and a liquid system for increasing a number of amplicons in clonal populations of amplicons at amplification sites. A reaction of the first solution and the second solution differing from it is carried out on a flow cell in the liquid system by flowing the first solution through amplification sites located on the flow cell, and then by flowing the second solution through amplification sites. The first solution includes target nucleic acids and the first mixture of reagents, which includes nucleoside triphosphates and replication enzymes. Target nucleic acids in the first solution are transferred to amplification sites and bound to them at a transfer rate. Under the action of the first mixture of reagents, amplification occurs and amplicons are formed at an amplification rate that exceeds the transfer rate. The second solution includes the second mixture of reagents and does not contain any target nucleic acids. The second solution is intended for increasing the number of amplicons. Additional amplicons are formed by exclusion amplification at the amplification rate that exceeds the transfer rate.EFFECT: inventions increase the number of amplicons in clonal populations or clusters at amplification sites, providing more complete formation of amplification clusters, and also provide a result with significantly higher intensity and higher signal/noise ratio, increasing the accuracy and/or efficiency of sequencing and analysis of the genetic library.16 cl, 14 dwg, 3 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 759 690 C2 (51) МПК C12Q 1/6848 (2018.01) B01L 3/00 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (52) СПК B01L 3/5025 (2021.08); C12Q 1/6848 (2021.08); C12Q 2565/501 (2021.08); C12Q 2565/629 (2021.08); B01L 2300/0877 (2021.08) (21)(22) Заявка: 2019116874, 15.12.2017 15.12.2017 Дата регистрации: 16.11.2021 05.01.2017 US 62/442,680; 24.03.2017 ...

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РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2019 116 874 A (51) МПК C12Q 1/6848 (2018.01) B01L 3/00 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2019116874, 15.12.2017 (71) Заявитель(и): ИЛЛЮМИНА, ИНК. (US), ИЛЛЮМИНА КЕМБРИДЖ ЛИМИТЕД (GB) Приоритет(ы): (30) Конвенционный приоритет: 05.01.2017 US 62/442,680; 24.03.2017 GB 1704754.9 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 05.08.2019 US 2017/066751 (15.12.2017) (87) Публикация заявки PCT: WO 2018/128777 (12.07.2018) A Адрес для переписки: 197101, Санкт-Петербург, а/я 128, "АРСПАТЕНТ" R U (57) Формула изобретения 1. Способ, включающий: реакцию на проточной ячейке первого раствора и отличающегося от него второго раствора, проводимую посредством протекания первого раствора по массиву сайтов амплификации, находящихся на проточной ячейке, и затем посредством протекания второго раствора по массиву сайтов амплификации; где первый раствор включает совокупность нуклеиновых кислот-мишеней и первую смесь реагентов, которая включает нуклеозидтрифосфаты (НТФ) и один или более ферментов репликации, причем находящиеся в первом растворе нуклеиновые кислотымишени переносятся к сайтам амплификации и связываются с сайтами амплификации со скоростью переноса, и под действием первой смеси реагентов происходит амплификация нуклеиновых кислот-мишеней, которые прикреплены к сайтам амплификации, в результате чего получают клональные популяции ампликонов, полученных из соответствующих нуклеиновых кислот-мишеней, и при этом ампликоны образуются со скоростью амплификации, которая превышает скорость переноса; и второй раствор включает вторую смесь реагентов и не содержит нуклеиновых кислот-мишеней, причем второй раствор предназначен для увеличения количества ампликонов в клональных популяциях, находящихся на сайтах амплификации. 2. Способ по п. 1, в котором вторая смесь реагентов включает НТФ и один или более Стр.: 1 A 2 0 1 9 1 1 6 8 7 4 (54) АМПЛИФИКАЦИЯ БИБЛИОТЕК ...

НОВЫЙ СПОСОБ ПРОИЗВОДСТВА МЕТАНОЛА ИЗ НИЗКОКАЧЕСТВЕННОГО СИНТЕТИЧЕСКОГО ГАЗА

УСТРОЙСТВО ДЛЯ МИКРОСТРУКТУРИРОВАННОЙ ПРИВИВКИ БЕЛКОВ НА ПОДЛОЖКЕ

... 1. Устройство для микроструктурированной прививки нескольких белков на подложке, отличающееся тем, что оно содержит подложку (7), пленку, матрицу, источник света (9), оптическую систему, первый резервуар (1), предназначенный для вмещения первого водного раствора, второй резервуар (2), предназначенный для вмещения второго водного раствора, и микрожидкостной контур, пленка расположена на подложке, источник выполнен с возможностью освещения матрицы светом, матрица выполнена с возможностью распространять свет в соответствии с первым структурированным рисунком, матрица содержит оптические средства для замены первого структурированного рисунка вторым структурированным рисунком, оптическая система выполнена с возможностью сформировать на пленке первое микроструктурированное изображение первого рисунка, контур выполнен с возможностью содержания первого водного раствора, контур имеет отверстие, выполненное с возможностью приводить в контакт первый раствор с пленкой на уровне отверстия, контур содержит ...

Verfahren und Vorrichtung zur Mikrodosierung kleinster Flüssigkeitsmengen für Biopolymerarrays

The invention relates to a method and device for microdosing the smallest amounts of liquid for the production of biopolymer arrays. The sample liquids to be analyzed are delivered by means of a delivery device (1), which can be connected to a rinsing fluid store. A reversible electric voltage (10) can be applied to said delivery device (1) so that the self-adjusting electroosmotic flow can be used for transporting the sample liquids to a detection array.

MEHRFACH-BIOREAKTORVORRICHTUNG

Druckreaktionsblock

VERFAHREN UND VORRICHTUNG ZUM ÜBERFÜHREN VON LUFTEMPFINDLICHEN STOFFEN

CHEMICAL PROCEDURE MICRO-SYSTEMS FOR THE EVALUATION OF HETEROGENEOUS CATALYSTS AND YOUR USE

PROCESS OPTIMIZATION REACTOR WITH PARALLEL FLOW

MULTIPLE BIOREACTOR DEVICE

DEVICE FOR THE PRODUCTION OF OLIGOMEREN, IN PARTICULAR PEPTOIDEN, WITH FEEDBACK OF THE REAGENTS

MORE MULTISYNTHETISIERER AND SEMIAUTOMATIC SYNTHESIS PROCEDURE FOR THE PRODUCTION OF PEPTIDEN IN THE CELEBRATIONS THE PHASE.

DEVICE AND PROCEDURE FOR THE PARALELLEN TREATMENT OF A MAJORITY OF REACTION MIXTURES

MICRO-FLUID POORLY REACTIVE WITH THREE FLOW LEVELS

PARALLEL REACTOR WITH INTERNAL FEELING AND PROCEDURE FOR ITS USE

METHOD AND SYSTEM FOR THE DETECTION OF CARDIAC RISK FACTORS

(semi-) batch reaction system

Device for delivering defined volumes

Multiple fluid sample processor and system

COMBINATORIAL STRATEGIES FOR POLYMER SYNTHESIS

Array of autoclaves

MULTI-SYNTHETISEUR DE PEPTIDES SEMI-AUTOMATIQUE EN PHASE SOLIDE ET PROCEDE DE PRODUCTION DE PEPTIDES DE SYNTHESE MIS EN OEUVRE PAR LE MULTI-SYNTHETISEUR

La présente invention concerne un multi-synthéti-seur de peptides semi-automatique en phase solide et un procédé de production de peptides de synthèse mis en oeuvre par le multi-synthétiseur. Multi-synthétiseur caractérisé en ce qu'il est principalement constitué par des réacteurs chimiques, reliés à des doseurs volumétriques de solvant disposés en amont desdits réacteurs chimiques et en aval d'un sélecteur de solvant alimenté en solvant grâce à des bouteilles de solvant sous pression d'azote, par des cylindres de distribution, ainsi que par une centrale de réglage et de régulation d'azote très basse pression, ces moyens étant séquentiellement commandés par un automate programmable ou un micro-ordinateur assurant la mise en oeuvre de la technique de synthèse, et le nombre maximum d'ensembles doseur volumétrique de solvant-réacteur chimique étant égal au nombre maximum de peptides pouvant être fabriqués simultanément.

ELECTRO-PNEUMATIC DISTRIBUTOR FOR MULTIPLEXED .MU.-TAS DEVICES

... ▓▓▓An electrospray system is disclosed. The electrospray system includes a ▓microdevice (10) comprising wells (12), channels (16), and electrospray tips ▓(14); an electro-pneumatic distributor (22) comprising channels (28) and ▓electrodes (24); a supply block (36) comprising gas supply channel (34) and ▓electric conductor (32); and a gasket (18) with holes (20). The distributor is ▓suitable for simultaneous, selective application of pressure and electric ▓current to individual channels of a microdevice.▓ ...

DEVICE FOR THE MICROSTRUCTURED GRAFTING OF PROTEINS ONTO A SUBSTRATE

L'impression de plusieurs protéines en séquence ou l'une après l'autre, sur un substrat, de façon automatisable et industrielle est un problème difficile pour l'art antérieur. L'invention se rapporte à un dispositif pour le greffage micro-structuré de plusieurs protéines sur un substrat possédant des avantages pratiques de stabilité et d'adaptation et aussi étant susceptible à l'application industrielle pour l'impression des protéines sur un substrat, l'invention comprenant un substrat, une couche, une matrice, une source d'une lumière, un système optique, un premier réservoir destiné à recevoir une première solution aqueuse, un deuxième réservoir destiné à recevoir une seconde solution aqueuse et un circuit micro-fluidique. L'invention se rapporte de plus sur un procédé de greffage micro-structuré de protéines sur un substrat utilisant un dispositif de l'invention.

СПОСОБ ОЦЕНКИ ЗАГРЯЗНЕНИЯ, ВЫЗЫВАЕМОГО НЕФТЕЗАВОДСКИМ СЫРЬЕМ

В изобретении описан способ оценки воздействия загрязнения, вызванного нефтезаводским сырьем, на один или несколько процессов нефтепереработки, при осуществлении которого (i) подготавливают нефтезаводское сырье множества типов и/или множество фракций нефтезаводского сырья одного или нескольких типов; (ii) создают массив, включающий множество образцов металлов, используемых в металлических частях нефтезаводского оборудования; (iii) вводят каждый из образцов металла в контакт с упомянутым нефтезаводским сырьем или фракциями нефтезаводского сырья одного или нескольких типов при нестатических условиях и (iv) определяют степень загрязнения нефтезаводского сырья и/или его фракции. Образцы металлов предпочтительно используют в форме изготовленного средствами микротехнологии массива и путем высокопроизводительного экспериментирования осуществляют их параллельное испытание.

СПОСОБ ОЦЕНКИ СТЕПЕНИ ЗАГРЯЗНЕНИЯ, ВЫЗВАННОГО НЕФТЕЗАВОДСКИМ СЫРЬЕМ

Способ оценки влияния загрязнения, вызванного нефтезаводским сырьем, на один или несколько процессов нефтепереработки, при осуществлении которого (i) подготавливают нефтезаводское сырье множества типов и/или множество фракций нефтезаводского сырья одного или нескольких типов, (ii) создают массив множества образцов металлов, используемых в металлических частях нефтезаводского оборудования, (iii) вводят каждый из образцов металла в контакт с названным нефтезаводским сырьем или фракциями нефтезаводского сырья одного или нескольких типов при нестатических условиях и (iv) определяют степень загрязнения нефтезаводским сырьем и/или его фракциями. Образцы металлов преимущественно используют в форме изготовленного средствами микротехнологии массива и путем высокопроизводительного экспериментирования осуществляют их параллельное исследование.

METHOD AND AUTOMATIC DEVICE MULTI-REACTEURS Of EVALUATION OF CATALYSEURSAVEC a HEAVY LOAD

SEMI-AUTOMATIC PEPTIDE MULTI-SYNTHETISEUR IN SOLID PHASE

CATALYST TREATMENT APPARATUS AND PROCESS

An apparatus for the sequential and/or parallel treatment of a plurality of molecular sieves samples is provided. The apparatus includes at least one support means having attached thereto a plurality of sample holders. The support means is in fluid communication with the interior of each sample holder. The apparatus also includes a tube furnace for receiving the support means, a means for supplying an inert gas, air, oxygen or a mixture thereof to each sample holder for a selected time period, a programmable device for sequentially directing the flow of inert gas, air, oxygen or a mixture thereof to each support means and each sample holder attached to the support means, and a means for varying temperature of the tube furnace. The apparatus also includes a means for supplying an ion exchange liquid or a source of a metal to each support means and each sample holder attached to the support means. Alternative apparatus for ion exchange or metal loading of molecular sieves is provided. Processes ...

CRYSTAL GROWTH DEVICES AND SYSTEMS, AND METHODS FOR USING SAME

High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.

METHOD AND DEVICE FOR MAKING A SUPPORT BEARING A PLURALITY OF DIFFERENT POLYNUCLEOTIDE AND/OR PEPTIDE SEQUENCES

The invention concerns the manufacture of electronic micro-sensors (chips) for analysing nucleotide and/or peptide sequences. The object is to fix at the surface of said chips a very large number of various probe sequences easily and economically. The method aims at making arrays of sequence probes on a support by anchoring and/or local chemical synthesis in the presence of volatile solvents. Said method is characterised in that it consists in using a support whereon is produced a grid of reaction wells made of light-cured (epoxy) resin; in placing the support in a reaction chamber which is saturated with vapours of the synthesis solvent (acetonitrile); in finally producing the anchorage and optionally the localised chemical synthesis in situ of the probe sequences in the wells using a localised supply of liquids/reagents in each well corresponding to a specific probe-sequence, and in supplying collectively the wells with common liquids/reagents and in ensuring that prior to each localised ...

SYSTEMS AND METHODS FOR THE SYNTHESIS OF ORGANIC COMPOUNDS

The invention provides systems (10) and methods for synthesizing various compounds on solid support. According to one exemplary method, a synthesizer (12) is provided having at least one reaction vessel block (22) with a plurality of reaction vessels (30) therein. At least one solid support is introduced into at least some of the reaction vessels (30), and a reagent is introduced into the reaction vessels (30) having the solid supports. The reaction vessel block (22) is then vibrated while heat is supplied to the reaction vessel block (22) to heat the reaction vessels (30). Optionally, the synthesized compounds may be cleaved from the solid supports following synthesis while they remain within the reaction vessels (30).

APPARATUS FOR SYNTHESIS OF OLIGOMERS, ESPECIALLY PEPTOIDS, WITH REAGENT RECYCLING

A method is provided for the synthesis of an oligomer on a solid phase support using a submonomer solid phase chemical synthesis technique. The method entails a recycling step wherein unused reagent is returned to a reagent vessel after a displacement step has been carried out with the reagent, and by-product contaminants are optionally removed from the recycled reagent using a suitable scavenging agent. Also provided is an apparatus for conducting the methods of the invention, which apparatus includes plumbing and containment means for carrying out the synthesis and recycling steps.

Automated polypeptide synthesis process

An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another. The activator system also includes a temperature controlled concentrator vessel in which an activator solvent is replaced by a coupling solvent to enhance the coupling of the activated species to the peptide chain in the reaction vessel. Also included in the synthesizer ...

Process and assembly for simultaneously evaluating a plurality of catalysts

A process and an assembly for simultaneously evaluating a plurality of catalysts is provided wherein the flow rate of a reactive fluid to each of a plurality of reactors is automatically adjusted based on the measured amount of catalyst sample in each reactor to concurrently obtain a substantially identical fluid space velocity in each of the reactors.

Device for carrying out liquid reactions with fine-grained solid catalysts and method for the use thereof

A reactor for carrying out catalyzed liquid reactions in which the catalyst is present as a dispersion in the reaction zone. The reactor includes at least one inlet and outlet, with all starting materials being fed in via the inlet and all products being discharged via the outlet. The inlet and outlet can be switched so that an exit previously serving as the outlet is utilized as an entrance serving as the inlet and at the same time an entrance previously serving as the inlet is utilized as an exit serving as the outlet. The inlet and outlet each include a filter element that keeps the catalyst in the reactor. The reactor also includes a device that ensures homogeneous distribution of the catalyst and the starting materials in the reactor. This reactor can carry out reactions at a high solids content without the catalyst having to be separated off from a product stream in an extra process step and without regular cleaning and thus shutdown of the process being necessary.

Apparatus comprising polymers

A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protecting groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.

Pressurized reactor apparatus with magnetic stirring

An apparatus for parallel processing of reaction mixtures comprises a plurality of vessels for holding a plurality of reaction mixtures for processing. A plurality of vessel supports are adapted for supporting the plurality of vessels. Caps sealingly engage the vessel supports for sealing the vessels within the vessel supports. The vessels, vessel supports, and caps define reaction chambers having bearings and stirrers rotatable therein. The stirrers each comprise a spindle rotatable in a respective bearing and at least one stirring implement extending from the spindle for contacting a respective reaction mixture. The stirrers further comprise at least one magnet adapted to be subjected to a rotating magnetic field in the vessel for causing the stirrer to rotate thereby to mix the respective reaction mixture. The stirrers may further comprise a first magnetic follower, a second magnetic follower, and a flux guide for guiding magnetic flux between the first magnetic follower and the second ...

Systems and methods for the synthesis of organic compounds

The invention provides systems and methods for synthesizing various compounds on solid support. According to one exemplary method, a synthesizer is provided having at least one reaction vessel block with a plurality of reaction vessels therein. At least one solid support is introduced into a least some of the reaction vessels, and a reagent is introduced into the reaction vessels having the solid supports. The reaction vessel block is then vibrated while heat is supplied to the reaction vessel block to heat the reaction vessels. Optionally, the synthesized compounds may be cleaved from the solid supports following synthesis while they remain within the reaction vessels.

Sample wash station assembly

A wash station assembly and method for washing selected samples in a sample containing assembly. The wash station assembly includes a wash station having a frame and a fluid dispensing assembly connected to the frame. The fluid dispensing assembly has a selector valve that is connectable to a plurality of fluid lines and is adjustable to allow only one of the fluids to pass through the selector valve at a time. An array of syringes is coupled to the selector valve and positioned to receive the fluid passing through the selector valve. Each syringe includes a check valve that prevents backflow of fluid or air into the syringe through the pipetting needles. A distribution manifold is connected to the selector valve and receives the fluid passing through the selector valve. The manifold has a plurality of distribution channels coupled to the syringes, to direct the fluid into the syringes. Each distribution channel has a manifold valve therein that prevents backflow of fluid into the manifold ...

SMART COMBINATORIAL OPERANDO SPECTROSCOPY CATALYTIC SYSTEM

Flow rate monitoring device

分画化されたマイクロエレクトロニックデバイスアレイ

... 複数のテストまたは合成を並列に処理するためのシステムであって;試料のテストまたは合成のための1以上のチャネルによって接続された複数のウェルからなるマイクロラボラトリーアレイ内に、試料を移送するための試料チャネルと;該アレイ、および、該アレイ内の試料を測定するための少なくとも1つの検出器と、少なくとも1つの光源とからなる光学系を収容するためのステーションと;前記アレイを、該アレイ内への流体の流れをモニターおよび制御することが可能な装置に対して、電気的接続するための手段とを含む。試料は、通常の装填チャネルから前記アレイ内に装填され、前記ウェル内で処理され、前記光学系で測定される。該アレイは、多くの試料を処理したり、多くの化合物を並列に合成することができ、このようなプロセスに要する時間を低減する。 ...

СПОСОБ И УСТРОЙСТВО ДЛЯ ПОЛУЧЕНИЯ БИОПОЛИМЕРНЫХ ПОЛЕЙ

Предложенное решение относится к способу и устройству для получения биополимерных полей или матриц, нуклеиновых кислот, протеинов и/или полисахаридов для расположения проб этих субстанций на носитель или используемый в качестве носителя материал. Способ получения биополимерных полей на поверхности несущих субстратов заключается в том, что наносимые биополимеры забирают из одного или нескольких различных источников проб с помощью перемещаемого в многомерном пространстве капиллярного кончика капиллярной трубочки для переноса мельчайших количеств жидкости на поверхность субстрата. Управление капиллярной трубочкой осуществляют с помощью первого миниатюрного клапана для заполнения капиллярной трубочки запасом жидкости, причем одну или несколько капиллярных трубочек перемещают в направлениях Х и Y и ей или им сообщают движение погружения в направлении Z в, по меньшей мере, один источник проб для приема запаса жидкости за счет капиллярной силы и вторым миниатюрным клапаном для промывки капиллярной ...

Kombinatorische Strategien für die Polymersynthese

Kombinatiorische Strategien zur Polymersynthese

Flowable substance e.g. low viscous substance, dosing device for combinatory chemistry, has piston acting on container such that space of container is reduced by pressing container, and dosing valve arranged in base of container

The device has a container (1) enclosing a space that contains flowable substance. The space is compressible by a piston such that the substance flows out through an outlet (7) by the compression of the space. The container has a flexible wall (3), and the piston acts on the container such that the space is reduced by pressing the container. A dosing valve (9) is arranged in a base (5) of the container. A filling opening is positioned at an upper side of the container. An independent claim is also included for a method for operating a dosing device.

Apparatus and methods for performing parallel processes

Embodiments of the present invention are directed to an apparatus and method for performing parallel processes. One embodiment of the present invention is directed to an apparatus for performing a process having a plurality of steps in which each step is performed in a vessel (13a-13f) by flowing a fluid through said vessel as shown in the figure. The steps of the process comprise at least a first step and at least one subsequent step. At least one of the subsequent steps is a final step, and each step has a time associated with its performance, a step period. At least one step has a longest step period, and the total number of steps, times the longest step period equals a process period. The apparatus comprises a plurality of vessels (13a-13f). Each of the plurality of vessels is for performing sequentially all the steps of the process in the process period and each of the plurality of vessels is for performing each step of the process in the longest step period.

Fluid ejector system

APPARATUS FOR AUTOMATICALLY PERFORMING CHEMICAL PROCESSES

... 1307086 Rotary conveyers BECKMAN INSTRUMENTS Inc 31 March 1970 [8 April 1969] 15067/70 Heading B8A [Also in Division B1] As fraction collector comprises vacuum-tight cylindrical housing 166 having a transparent cover 275 and enclosing rotatable tube rack 279, tube rack 279 being attached by shaft 283 to gear 281 which co-operates with arm 282 driven by motor 163 to enable stepping of the tube rack. Shaft 283 is rotatably enclosed by ball-bearings 287, 288 in housing 284. Housing 284 is sealably mounted in the bottom wall of main housing 166 and an O-ring 289 in an annular groove on the outer surface of rotatable shaft 283 provides a vacuum-tight seal. between theshaft and housing 284. Tubular liquid inlet device 285 is movable linearly by motor 290 to enable the dispensing of liquid into the inner and outer rows of tubes 276.

Injector system

MICRO-FLUID DEVICES AND PROCEDURES FOR CHEMICAL SAMPLE

Devices and methods for interfacing microfluidic devices with macrofluidic devices

The present disclosure is directed generally to devices and methods with the purpose of interfacing microfluidic devices with macrofluidic devices. Specifically, the present disclosure includes the de-

KINETIC EXCLUSION AMPLIFICATION OF NUCLEIC ACID LIBRARIES

An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites. 1. A fluidic system , comprising:a reagent manifold including at least one valve in fluid communication with an inlet port of a flow cell that includes an array of amplification sites, the reagent manifold further including a plurality of channels fluidly connected between the at least one valve and corresponding reagent reservoirs; anda controller including one or more processors, the controller to control the at least one valve and a pump to flow a first solution through the inlet port over the array of amplification sites on the flow cell and to subsequently flow a different, second solution through the inlet port over the array of amplification sites on the flow cell;wherein the first solution includes a number of target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates (NTPs) and one or more replication enzymes, the number of target nucleic acids in the first solution exceeding a number of the amplification sites in the array, the first solution reacting on the flow cell to ...

Integrated Analysis System

The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.

APPARATUS AND METHOD FOR ANALYZING REACTIONS

The invention proceeds from an apparatus for analyzing reactions, comprising a starting material distributor () and at least two reactors () which are connected in parallel and are each connected via a connecting conduit () to an outlet of the starting material distributor (). To set the inflow, a pressure regulator () and a restrictor () are installed in each connecting conduit () between the starting material distributor () and the reactors () or an outlet conduit () in which a restrictor () and a pressure regulator () are installed branches off from each connecting conduit (). 1735733195731319335. An apparatus for analyzing reactions , comprising a starting material distributor () and at least two reactors () which are connected in parallel and are each connected via a connecting conduit () to an outlet of the starting material distributor () , wherein a pressure regulator () and a restrictor () are installed in each connecting conduit () between the starting material distributor () and the reactors () in order to set the inflow or an outlet conduit () in which a restrictor () and a pressure regulator () are installed branches off from each connecting conduit ().233719. The apparatus according to claim 1 , wherein the pressure regulator () is an exit pressure regulator and is installed between the starting material distributor () and the restrictor ().333193. The apparatus according to claim 1 , wherein the pressure regulator () is an admission pressure regulator and is installed between the restrictor () and the reactor ().419. The apparatus according to claim 1 , wherein the restrictor () is a capillary claim 1 , a microstructured component claim 1 , an orifice plate or a nozzle.539. The apparatus according to claim 1 , wherein the reactors () are connected to a further distributor ().61993. The apparatus according to claim 1 , wherein a restrictor () is positioned between the further distributor () and each reactor ().7339191993. The apparatus according to ...

AUTOMATIC GAS VALVE CONTAINER HOLDER FOR CHEMICAL SYNTHESIS

The invention relates to a container holder comprising a main body which in turn comprises a gas inlet a solution liquid outlet a gas control valve through which a gas enters the container from the gas inlet; and a sealing means for the container, which sealing means includes a passageway for the input of gas and output of a solution in the container via an egress tube wherein when the container is connected to the container holder through the sealing means, the gas control valve opens automatically, and when the container is disconnected the gas control valve is closed automatically. The invention further relates to a container panel which includes two or more container holders. Also disclosed are methods of using these containers and container panels for synthesizing a polypeptide. 1. A container holder , comprising a main body which in turn comprises:(a) a gas inlet;(b) a solution outlet;(c) a gas control valve which allows a gas to enter the container from the gas inlet; and(d) a sealing means for sealing the container in use, which sealing means includes a passageway for the input of the gas and for the egress of a liquid in the container;wherein, when the container is sealingly connected to the container holder by the sealing means, the gas control valve opens automatically, and when the container is disconnected from the container holder the gas control valve is closed automatically.2. The container holder of claim 1 , wherein the gas control valve is a spring valve operable by means of a plate claim 1 , in turn displaced be the act of sealingly connecting the container to the container holder.3. The container holder of claim 1 , wherein the main body includes an internally threaded aperture and wherein the sealing means comprises a threaded part complementary to the threaded aperture and a plate claim 1 , wherein when the container is connected with the threaded part claim 1 , the plate opens the gas control valve.4. The container holder of claim 2 , wherein ...

REACTOR LAYOUT FOR METHANOL PRODUCTION FROM LOW QUALITY SYNTHESIS GAS

A reactor layout for a process of methanol production from low quality synthesis gas, in which relatively smaller adiabatic reactors can be operated more efficiently, some of the inherent disadvantages of adiabatic reactors for methanol production are avoided. This is done by controlling the outlet temperature in the pre-converter by rapid adjustment of the recycle gas, i.e. by manipulating the gas hourly space velocity in the pre-converter. 1. A reactor layout for carrying out the process for methanol production from synthesis gas , said reactor layout comprising:one or more methanol pre-converters/guard reactors, in which the synthesis gas is partially converted to methanol over a heterogeneous catalyst to generate an effluent gas without impurities,a feed-effluent heat exchanger for cooling the effluent gas from the one or more methanol pre-converters/guard reactors, and a main methanol converter for converting a mixture of recycle gas and the cooled effluent gas from the one or more methanol pre-converters/guard reactors,', 'a main feed-effluent heat exchanger,', 'a cooler or a series of coolers,', 'a high pressure gas-liquid separator, which splits the inlet flow into raw methanol and recycle gas,', 'a recycle compressor, and', 'a loop purge., 'a methanol synthesis loop comprising2. The reactor layout according to claim 1 , said reactor layout further comprising means for defining and controlling a maximum allowable outlet temperature using a cold recycle gas stream which is injected into the one or more pre-converters/guard reactors via a control valve.3. The reactor layout according to claim 1 , which has fresh feed gas splitting located on the cold side claim 1 , before the feed-effluent heat exchanger.4. The reactor layout according to claim 1 , which enables on-stream catalyst replacement in the one or more pre-converters/guard reactors. This is a divisional of application Ser. No. 15/749,607, filed on Feb. 1, 2018, which is a 371 of PCT/EP2016/066877, ...

Methods and devices for high fidelity polynucleotide synthesis

Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.

Kinetic exclusion amplification of nucleic acid libraries

An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites.

MODULAR REACTOR SYSTEMS AND DEVICES, METHODS OF MANUFACTURING THE SAME AND METHODS OF PERFORMING REACTIONS

Aspects of the present invention provide a modular reactor device having an outer housing, and a plurality of components contained within the outer housing, the components including: a reaction chamber; a fluid pathway connected to the reaction chamber; and a valve arranged to control flow of fluid within the device, wherein the outer housing has a plurality of connection ports providing connections from the exterior of the device to the interior, the connection ports including: a fluid input and a fluid output; an electrical input; and a pneumatic input; wherein either the electrical input or the pneumatic input is connected to the valve to provide for control of the valve, and either the fluid input or the fluid output is connected to the reaction chamber or the fluid pathway. Other aspects provide a base station for receiving and controlling a modular reactor device and methods for manufacturing the modular reactor device and for performing reactions using a modular reactor device. 1. A modular reactor device having an outer housing , and a plurality of components contained within the outer housing , the components including:a reaction chamber;a fluid pathway connected to the reaction chamber; anda valve arranged to control flow of fluid within the device,wherein the outer housing has a plurality of connection ports providing connections from the exterior of the device to the interior, the connection ports including:a fluid input and a fluid output;an electrical input; anda pneumatic input;wherein either the electrical input or the pneumatic input is connected to the valve to provide for control of the valve, andeither the fluid input or the fluid output is connected to the reaction chamber or the fluid pathway.2. A modular reactor device according to wherein the plurality of components further includes a storage compartment and a fluid pathway connecting the storage compartment and the reaction chamber.3. A modular reactor device according to wherein at least ...

METHODS AND DEVICES FOR HIGH FIDELITY POLYNUCLEOTIDE SYNTHESIS

Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products. 1. A method for assembling a polynucleotide having a predefined sequence from a plurality of different oligonucleotides , the method comprising:a) providing a plurality of single-stranded template oligonucleotides on a support, wherein each of the plurality of template oligonucleotides comprises a predefined sequence and includes a primer binding site;b) generating a complementary oligonucleotide for each of the plurality of template oligonucleotides by enzyme-catalyzed synthesis within a primary droplet, thereby producing a plurality of double-stranded oligonucleotides;c) releasing the complementary oligonucleotide from the double-stranded oligonucleotides into the primary droplet;d) combining at least a first and second primary droplets, thereby forming a secondary droplet, wherein the first primary droplet includes a released oligonucleotide that comprises a portion that is complementary to a portion of a released or template oligonucleotide from the second primary droplet; ande) exposing the secondary droplet to conditions suitable for hybridization and ligation, polymerase extension, or polymerase extension and ligation to assemble a double-stranded polynucleotide having a predefined sequence.2. The method of wherein the plurality of single-stranded template ...

NOVEL PROCESS FOR METHANOL PRODUCTION FROM LOW QUALITY SYNTHESIS GAS

In a novel process for methanol production from low quality synthesis gas, in which relatively smaller adiabatic reactors can be operated more efficiently, some of the inherent disadvantages of adiabatic reactors for methanol production are avoided. This is done by controlling the outlet temperature in the pre-converter by rapid adjustment of the recycle gas, i.e. by manipulating the gas hourly space velocity in the pre-converter. 1. A process for methanol production from synthesis gas comprising the following steps:providing a fresh, pressurized methanol synthesis gas containing hydrogen, carbon monoxide and carbon dioxide, which is pre-heated and passed through a methanol pre-converter/guard reactor, in which the synthesis gas is partially converted to methanol over a heterogeneous methanol catalyst and poisonous impurities are removed from the feed gas,providing a recycle gas stream containing partly converted methanol synthesis gas and mixing a part of the recycle stream with the fresh synthesis gas if necessary, such as in case of part load, to a process gas stream,cooling the process gas stream from the pre-converter/guard reactor to a temperature, which is suitable for the main converter, and passing the cooled process gas stream to a conventional methanol synthesis loop, andseparating raw methanol from the synthesis loop,wherein the outlet adiabatic temperature in the pre-converter/guard reactor is controlled by rapid adjustment of the recycle gas, i.e. by manipulation of the GHSV (gas hourly space velocity) in the pre-converter.2. Process according to claim 1 , wherein the pre-converter/guard reactor(s) is/are sized and designed for the plant full load condition claim 1 , treating and converting the inlet feed gas (make-up gas) without risk of catalyst overheating.3. Process according to claim 2 , wherein a safe gas space velocity is established across the reactor in the methanol synthesis loop to avoid overheating of the catalyst.4. Process according to ...

Microfluidic Device for the Generation of Combinatorial Samples

The present disclosure relates to a microfluidic device and a method allowing the generating and screening of combinatorial samples. A microfluidic device for producing droplets of at least one sample into an immiscible phase is provided, the device comprising a droplet maker connecting an immiscible phase channel and a sample channel having at least one sample inlet connected to at least one sample inlet channel injecting the at least one sample into the sample channel, wherein the injection of the at least one sample is controlled by at least one sample valve, so that the at least one sample flows either towards a sample waste outlet or into the at least one sample inlet channel, wherein different sample inlet channel of the at least one sample inlet channel have the same hydrodynamic resistance resulting from the length, height and width of each sample inlet channel upstream of the droplet maker. 1. A microfluidic device for producing droplets of at least one sample into an immiscible phase , the device comprising:a droplet maker connecting an immiscible phase channel and a sample channel having a plurality of sample inlet connected to a plurality of sample inlet channel injecting the at least one sample into the sample channel, wherein the injection of the at least one sample is controlled by a plurality of sample valves, so that the at least one sample flows either towards a sample waste outlet or into one of the plurality of sample inlet channels, wherein different ones of the plurality of sample inlet channels have the same hydrodynamic resistance resulting from the length, height and width of the sample inlet channel upstream of the droplet maker; and an outlet channel, wherein the hydrodynamic resistance of the outlet channel is lower than the hydrodynamic resistance of the immiscible phase channel.2. The microfluidic device of claim 1 , wherein ones of the plurality of sample inlet channels have a sample fluidic resistor to adjust length.3. The ...

A fluidic device for aliquoting and combinatorial mixing of liquids

The present invention relates to fluidic devices, especially microfluidic devices, for aliquoting and pairwise combinatorial mixing of a first set of liquids with a second set of liquids. The device architecture is designed to move liquids in two separate phases, a first phase where the liquids are exposed to a first directional force field to move the liquids in a first direction, from a reservoir to aliquot chambers, and a second phase where the liquids are exposed to a second directional force field to move the liquids in a second direction, from the aliquot chambers to the mixing chambers. The first and second directional force fields that the device is exposed to may be achieved using a single directional force field (i.e. a rotor driven centrifugal force field) and by re-orienting the position of the device with respect to the centrifugal forces between the first and second phases of operation. The device architecture comprises reservoirs for each of the first fluids and reservoirs for each of the second fluids. Each reservoir is fluidically connected to aliquoting chambers, either arranged in parallel or in series, for providing aliquots of the fluid which may be metered. The conduits providing fluid communication between the reservoirs and aliquoting chambers are arranged in a first direction. A series of mixing chambers is also provided, and each mixing chamber is fluidically connected to one aliquot chamber for a first liquid and one aliquoting chamber for a second liquid. The conduits providing fluid communication between the aliquoting chambers and mixing chambers are arranged in a second direction.

APPARATUS FOR ASSAY, SYNTHESIS AND STORAGE, AND METHODS OF MANUFACTURE, USE, AND MANIPULATION THEREOF

The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples. 150-. (canceled)51. A device for filling through-holes in a platen , the device comprising:a platen comprising a two-dimensional array of through-holes, each of the through-holes configured to receive at least one reagent;a nozzle having an aperture of a suitable size to inject a sample into a plurality of the array of through-holes; anda valve configured to control a flow of a sample through the nozzle;wherein the nozzle is configured to move in at least two dimensions to predetermined positions over respective ones of the plurality of through-holes.52. The device of claim 51 , wherein the nozzle is positioned so as to contact the platen.53. (canceled)54. The device of claim 51 , further comprising a computer that controls the valve and controls the predetermined positions of the nozzle over the plurality of through-holes.5563-. (canceled)64. A method of storing a plurality of samples in an assay-ready claim 51 , high-density format claim 51 , the method comprising:a) providing a platen having a plurality of through-holes;b) loading the through-holes with said samples dissolved in a mixture comprising two solvents, a first solvent having a low vapor pressure and a second solvent having a higher vapor pressure; andc) evaporating the second solvent to result in a plurality ...

Process and apparatus for transferring air-sensitive substances

A process is provided for transferring and releasing substances which are air-sensitive or moisture-sensitive or light-sensitive, or air-sensitive and moisture-sensitive, or air-sensitive and light-sensitive, or moisture-sensitive and light-sensitive, or air-sensitive, moisture-sensitive and light-sensitive, at least one substance ( 20 ) which is air-sensitive or moisture-sensitive or light-sensitive, or air-sensitive and moisture-sensitive, or air-sensitive and light-sensitive, or moisture-sensitive and light-sensitive, or air-sensitive, moisture-sensitive and light-sensitive. The process comprises removing the substances after preparation under a protective gas atmosphere, from a synthesis chamber, wherein, during said removing, the substances are surrounded by at least one means ( 32, 34 ) for protecting the substances and then transferring the substances to at least one test reactor ( 50, 60 ), wherein the transfer takes place in a protective-gas-free chamber and at least one substance ( 20 ) is freed in the test reactor ( 50, 60 ) from the means ( 32, 34 ) protecting it using at least one apparatus or by means of at least one process.

Microfluidic assay devices and methods

A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.

Method and system for the detection of cardiac risk factors

A system for the rapid characterization of multi-cardiovascular risk factor analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.

Microfluidic assay devices and methods

A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.

Combinatorial strategies for polymer synthesis

A method for producing arrays by depositing a resist on a substrate, selectively removing a portion of the resist to expose localized areas, dispensing a monomer to occupy a localized area of less than 1 cm 2 of the surface of the support, allowing the monomer to bind directly or indirectly to the support and repeating the steps to produce an array of at least 10 different polymers is formed.

Combinatorial strategies for polymer synthesis

A method and device for forming large arrays of polymers on a substrate ( 401 ). According to a preferred aspect of the invention, the substrate is contacted by a channel block ( 407 ) having channels ( 409 ) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage ( 403 ), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

Apparatus comprising polymers

A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protecting groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.

Combinatorial strategies for polymer synthesis

A method and device for forming large arrays of polymers on a substrate ( 401 ). According to a preferred aspect of the invention, the substrate is contacted by a channel block ( 407 ) having channels ( 409 ) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage ( 403 ), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

Combinatorial strategies for polymer synthesis

A method for producing arrays by spacing a dispenser a distance from a surface of a support, dispensing a volume containing a compound in a single coupling step of less than 5 nl to occupy a localized area of less than 1 cm 2 of the surface of the support, allowing the compound to bind directly or indirectly to the support and repeating the steps to produce an array of at least 100 ligands at a density of 1000 per cm 2 or greater.

Combinatorial strategies for polymer synthesis

A method and device for forming large arrays of polymers on a substrate ( 401 ). According to a preferred aspect of the invention, the substrate is contacted by a channel block ( 407 ) having channels ( 409 ) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage ( 403 ), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodologies.

Combinatorial strategies for polymer synthesis

A method for producing polymer arrays by spacing a dispenser a distance from a surface of a support, dispensing a volume containing a monomer in a single coupling step of less than 5 nl to occupy a localized area of less than 1 cm 2 of the surface of the support, allowing the monomer to bind directly or indirectly to the support and repeating the steps to produce an array of at least 100 polymer ligands at a density of 1000 per cm 2 or greater.

Micro-fluidic heating system

Provided is a micro-fluidic heating system, which comprises a micro-fluidic control element for providing a chamber, a flow path and a valve, and a main body for heating the inside of the chamber in contact with the micro-fluidic control element, wherein the micro-fluidic control element consists of an upper substrate for providing the chamber, the flow path and the valve, and a lower substrate as a thin film bonded to the upper substrate, and the main body consists of a membrane in which heating means and suction holes are formed, and support member for supporting the membrane, and the heating means is partially in contact with the lower substrate of the chamber to heat the inside of the chamber, so that thermal transfer efficiency becomes maximized and temperature of each chamber may be independently controlled in the case of configuration having chambers arranged in array.

Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces

Substrates with surfaces comprising compounds with thiol functional groups protected with a photoremovable protecting group can be used to construct arrays of immobilized anti-ligands, such as oligonucleotide probes or other biological polymers. The arrays can be used in assays to detect the presence of complementary nucleic acids in a sample. Spatially addressed irradiation of predefined regions on the surface permits immobilization of oligonucleotides and other biological polymers at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allow formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix.

Chemical assays

An assay device in which to carry out a fluid-phase chemical assay, comprising means for supporting a test substrate, a sample chamber for receiving a fluid sample and at least one fluid control device for controlling the movement of fluid into and/or out of the sample chamber, wherein the fluid control device comprises a fluid outlet chamber in fluid communication with the sample chamber, and a displaceable flexible diaphragm the displacement of which alters the volume of the outlet chamber so as to allow and/or restrict fluid flow between the outlet and sample chambers. The invention also provides assay apparatus incorporating such a device, an assay station for use as part of such apparatus, a fluid control unit for use as part of the assay device and a method of conducting an assay which may involve the use of such apparatus and devices.

Method and system for the detection of cardiac risk factors

A system for the rapid characterization of multi-cardiovascular risk factor analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.

Methods for analysis of heterogeneous catalysts in a multi-variable screening reactor

Protocols for designing and implementing sets of simultaneous experiments, in a parallel, multi-variable process optimization reactor, are disclosed. The multi-variable process optimization reactor is preferably a parallel flow reactor having the operational capability to simultaneously vary reaction conditions between reaction vessels—either modularly or independently. The simultaneously varied reaction conditions preferably include at least two of the following, in various combinations and permutations: space velocity, contact time, temperature, pressure and feed composition. Compositional variations in the catalysts residing in each of the reaction vessels can also be investigated in the set of simultaneous experiments implemented in the parallel reactor. Sufficient data is obtained from a single set of simultaneous experiments to generate a master curve.

Method and device for transferring air-sensitive substances

Die vorliegende Erfindung betrifft ein Verfahren zur Überführung und Freisetzung von Substanzen, die luft- oder feuchtigkeits- oder lichtempfindlich oder luft- und feuchtigkeitsempfindlich oder luft- und lichtempfindlich oder feuchtigkeits- und lichtempfindlich oder luft-, feuchtigkeits- und lichtempfindlich sind, wobei wenigstens eine Substanz (20), die luft- oder feuchtigkeits- oder lichtempfindlich oder luft- und feuchtigkeitsempfindlich oder luft- und lichtempfindlich oder feuchtigkeits- und lichtempfindlich oder luft-, feuchtigkeits- und lichtempfindlich, nach ihrer Herstellung unter Schutzgasatmosphäre ihren Syntheseraum umgeben von wenigstens einem sie schützenden Mittel (32, 34) verläßt und anschließend in wenigstens einen Untersuchungsreaktor (50, 60) überführt wird, DOLLAR A dadurch gekennzeichnet, DOLLAR A daß die Überführung in einem schutzgasfreien Raum stattfindet und daß die wenigstens eine Substanz (20) im Untersuchungsreaktor (50, 60) mit Hilfe wenigstens einer Vorrichtung oder mittels wenigstens einem Verfahren von dem sie schützenden Mittel (32, 34) befreit wird. The present invention relates to a process for the transfer and release of substances which are sensitive to air or moisture or light or sensitive to air and moisture or sensitive to air or light or sensitive to moisture and light or sensitive to air, moisture and light, at least one substance (20) who are sensitive to air or moisture or light or sensitive to air or moisture or sensitive to air or light or sensitive to moisture and light or sensitive to air, moisture and light, after their manufacture in a protective gas atmosphere, their synthesis space surrounded by at least one protective agent (32, 34) and then transferred to at least one test reactor (50, 60), DOLLAR A characterized, DOLLAR A that the transfer takes place in a protective gas-free space and that the at least one substance (20) in the test reactor (50, 60 ) with help at least a device or by means of at least one method ...

Parallel reactor with internal sensing and method of using same

An apparatus and method for carrying out and monitoring the progress and properties of multiple reactions is disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight.

Parallel reactor with internal sensing

An apparatus and method for carrying out and monitoring the progress and properties of multiple reactions is disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel, and a system for injecting liquids into the vessels at a pressure different than ambient pressure. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight.

Apparatus for preparing oligomer

Provided is an apparatus for preparing oligomer including: a reactor for carrying out oligomerization reaction by supplying a monomer stream and a solvent stream; and line 1 and line 2 which are separately provided in a lower side of the reactor, wherein line 1 includes a first level control valve and line 2 includes a second level control valve, and the reactor is periodically alternately operated in first operation mode and second operation mode, thereby switching a pipe through which the product is discharged, so that a plugging phenomenon of the pipe through which the product is discharged and the valve can be prevented.

Methods and apparatus for characterization of polymers using multi-dimensional liquid chromatography with regular second-dimension sampling

Methods and apparatus for characterizing a polymer sample and in preferred embodiments, libraries of polymer samples, in a comprehensive, directly-coupled multi-dimensional liquid chromatography system are disclosed. The first and second dimensions are preferably high-performance liquid chromatography dimensions, such as for example, a first dimension adapted for determining composition (e.g. adapted for mobile-phase gradient elution chromatography, including reverse phase chromatography, adsorption chromatography and the like), and a second dimension adapted for determining molecular weight or particle size (e.g., adapted for size exclusion chromatography, including gel permeation chromatography).

Methods and apparatus for characterization of polymers using multi-dimensional liquid chromatography with regular second-dimension sampling

Methods and apparatus for characterizing a polymer sample and in preferred embodiments, libraries of polymer samples, in a comprehensive, directly-coupled multi-dimensional liquid chromatography system are disclosed. The first and second dimensions are preferably high-performance liquid chromatography dimensions, such as for example, a first dimension adapted for determining composition (e.g. adapted for mobile-phase gradient elution chromatography, including reverse phase chromatography, adsorption chromatography and the like), and a second dimension adapted for determining molecular weight or particle size (e.g., adapted for size exclusion chromatography, including gel permeation chromatography).

Parallel reactor with internal sensing and method of using same

Devices and methods for controlling and monitoring the progress and properties of multiple reactions are disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also for conventional experimental reactors as well. The apparatus generally includes multiple vessels (102, 206, 236, 266, 338, 362, 396, 522, 710, 876, 912, 1286, 1542) for containing reaction mixtures, and systems (180, 230, 260, 270, 272, 500, 1544, 1546, 1548, 1604) for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel, and a system (146, 1002) for injecting liquids into the vessels at a pressure different than ambient pressure. In situ monitoring of individual reaction mixtures provides feedback for process controllers (160, 268, 678, 720, 928, 1500, 1508, 1604), and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight. Computer-based methods are disclosed for process monitoring and control, and for data display and analysis.

Method of forming arrays of polymers

A method of forming a plurality of polymers having diverse monomer sequences on a substrate, the substrate including a plurality of reaction regions surrounded by a constraining region, the reaction regions being more wettable by one or more monomer solutions than the constraining region, the method comprising sequentially placing the one more monomer solutions in a first reaction region to form a first polymer having a first monomer sequence, the monomer solutions be confined to the first reaction region by the constraining region; and sequentially placing the one or more monomer solutions in a second reaction region to form a second polymer having a second monomer sequence, the monomer solutions being confined to the first reaction region by the constraining region.

Parallel reactor with internal sensing and method of using same

Devices and methods for controlling and monitoring the progress and properties of multiple reactions are disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel, and a system for injecting liquids into the vessels at a pressure different than ambient pressure. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight. Computer-based methods are disclosed for process monitoring and control, and for data display and analysis.

Analysis and control of parallel chemical reactions

Computer programs and computer-implemented methods for monitoring the progress and properties of parallel chemical reactions. The invention repeatedly receives a measured value or values associated with the contents of each of a plurality of reactor vessels and displays the measured over the course of a combinatorial chemical reaction. Reaction parameters associated with individual reactor vessels are changed in response to the value measured during the reaction. Reaction parameters include temperature, pressure, stirring speed. The reaction occurring in one or more reactor vessels is quenched in response to values measured during the reaction. The measured values are used to calculate experimental results including temperature change, pressure change, percent conversion of starting material, and viscosity. The measured values and experimental results are displayed. In another aspect, the invention features a method for controlling a combinatorial chemical reactor. The method includes receiving set points for properties associated with the reaction environment in multiple reactor vessels, measuring experimental values associated with each reactor vessel, displaying the experimental values, and changing the vessels' reaction environment in response to input set points and changing experimental values. In another aspect, the invention features a reactor control system for monitoring and controlling a parallel chemical reaction. The system includes modules for providing control signals to a parallel chemical reactor, receiving measured values from the parallel chemical reactor and calculating experimental results from the measured values, and for receiving reaction parameters from the user and displaying the set of measured values and the calculated values.

Parallel flow process optimization reactor

Parallel flow reaction systems comprising four or more reaction channels are disclosed. Distribution systems, and parallel flow reaction systems comprising such distribution systems are also disclosed. Specifically, the distribution systems comprise one or more subsystems, including for example, a flow-partitioning subsystem for providing a different flow rate to each of the four or more reactors, a pressure-partitioning subsystem for providing a different reaction pressure in the reaction cavity of each of the four or more reactors, and a feed-composition subsystem for providing a different feed composition to each of the four or more reactors. In preferred embodiments, the one or more subsystems can comprise at least one set of four or more flow restrictors, each of the four or more flow restrictors having a flow resistance that varies relative to other flow restrictors in the set.

用于测量并联反应器的流体流量的流动速度的系统和方法

本发明涉及一种用于测量并联反应器的流体流量的流动速度的系统，包括：-公共供给线，-多个用于接收反应器流体流量的反应器供给线，-测量线，以及-阀系统，所述阀系统包括一个或多个阀以及用于控制所述一个或多个阀的阀控制单元，所述阀系统设置为和/或适应于使得所述阀系统能够承担测量设置，其中所述阀重新定向反应器供给流量中的一个，使得所述反应器供给流量流过所述测量线。

用于表征催化过程的设备和方法

本发明涉及一种表征催化过程的方法，其包括具有平行布置的两个或更多个反应链的反应体系，其中各反应链包括多个串联的反应室或单个反应室。在也称为CKP方法的方法中，向各反应链提供反应物流。提供给反应链的反应物流在不同的反应链中经历不同数量的工艺步骤。对从反应链排出的产物流进行分析表征，其中表征中获得的数据以相对术语表示，此处优选包括差值计算。CKP方法可以以非常通用的方式使用，并且特征在于非常高的精度。质量平衡达到+/‑10％重量或更低的标准偏差。此外，本发明涉及一种用于实施CKP方法的装置，或者涉及一种用于同时实施多种CKP方法的装置。因此，本发明还涉及高通量研究领域。

核酸文库的动力学排除扩增

示例方法包括通过使第一溶液流过在流动池上的扩增位点使第一溶液在流动池上反应以及随后使不同的第二溶液流过扩增位点使第二溶液在流动池上反应。第一溶液包含靶核酸和第一试剂混合物，该第一试剂混合物包含核苷三磷酸和复制酶。第一溶液中的靶核酸以一个转运速率转运至扩增位点并且与扩增位点结合。第一试剂混合物扩增与扩增位点结合的靶核酸，以产生源自对应的靶核酸的扩增子的克隆群体。扩增子以超过转运速率的扩增速率产生。第二溶液包含第二试剂混合物，并且缺乏靶核酸。第二溶液用于增加扩增位点处的扩增子的数目。

Multi-temperature modular reactor and method of using same

An apparatus and method for carrying out and monitoring the progress and properties of multiple reactions is disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight.

Parallel semicontinuous or continuous reactors

Parallel semi-continuous or continuous reactors are disclosed. The parallel reactors preferably comprise four or more reaction vessels. The reaction vessels are preferably small volume reaction vessels, preferably pressure reaction vessels, and/or preferably integral with a common reactor block. The reaction vessels can comprise shaft-driven stirrers. At least two, preferably at least three or at least four liquid feed lines can provide selective fluid communication between each of the reaction vessels and one or more liquid reagent sources. Additional features, suitable in connection with parallel reactors or with single reaction vessels are also disclosed.

Parallel reactor with internal sensing and method of using same

Devices and methods for controlling and monitoring the progress and properties of multiple reactions are disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel, and a system for injecting liquids into the vessels at a pressure different than ambient pressure. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight. Computer-based methods are disclosed for process monitoring and control, and for data display and analysis.

Parallel reactor with sensing of internal properties

An apparatus and method for carrying out and monitoring the progress and properties of multiple reactions is disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel, and a system for injecting liquids into the vessels at a pressure different than ambient pressure. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight.

Parallel reactor with internal sensing and method of using same

Devices and methods for controlling and monitoring the progress and properties of multiple reactions are disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel, and a system for injecting liquids into the vessels at a pressure different than ambient pressure. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight. Computer-based methods are disclosed for process monitoring and control, and for data display and analysis.

Parallel reactor with internal sensing and method of using same

Devices and methods for controlling and monitoring the progress and properties of multiple reactions are disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel, and a system for injecting liquids into the vessels at a pressure different than ambient pressure. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight. Computer-based methods are disclosed for process monitoring and control, and for data display and analysis.

Multi-temperature modular reactor and method of using same

An apparatus and method for carrying out and monitoring the progress and properties of multiple reactions is disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight.

Parallel semicontinuous or continuous reactors

Parallel semi-continuous or continuous reactors are disclosed. The parallel reactors preferably comprise four or more reaction vessels. The reaction vessels are preferably small volume reaction vessels, preferably pressure reaction vessels, and/or preferably integral with a common reactor block. The reaction vessels can comprise shaft-driven stirrers. At least two, preferably at least three or at least four liquid feed lines can provide selective fluid communication between each of the reaction vessels and one or more liquid reagent sources. Additional features, suitable in connection with parallel reactors or with single reaction vessels are also disclosed.

Methods for sampling from non-atmospheric vessels in a parallel reactor system

Methods for sampling reactor contents in parallel reactor systems are disclosed. The methods may be used to sample reactor contents in non-atmospheric (e.g., pressurized) reaction vessels.

Multi-temperature modular reactor and method of using same

An apparatus and method for carrying out and monitoring the progress and properties of multiple reactions is disclosed. The method and apparatus are especially useful for synthesizing, screening, and characterizing combinatorial libraries, but also offer significant advantages over conventional experimental reactors as well. The apparatus generally includes multiple vessels for containing reaction mixtures, and systems for controlling the stirring rate and temperature of individual reaction mixtures or groups of reaction mixtures. In addition, the apparatus may include provisions for independently controlling pressure in each vessel. In situ monitoring of individual reaction mixtures provides feedback for process controllers, and also provides data for determining reaction rates, product yields, and various properties of the reaction products, including viscosity and molecular weight.

A PARTITIONED MICROELECTRONIC DEVICE ARRAY

다수의 병렬 테스트 또는 분석을 처리하기 위한 시스템은 샘플의 상기 테스팅 또는 분석을 위해 하나 이상의 채널에 의해 접속된 다수의 웰에 대한 마이크로 실험 어레이내로 상기 샘플을 이동하기 위한 채널, 상기 어레이를 하우징하기 위한 스테이션과 상기 어레이내의 상기 샘플을 측정하기 위한 적어도 하나의 광소스 및 광검출기를 포함하는 광학 시스템, 및 상기 어레이내의 유체의 흐름을 모니터링하고 제어할 수 있는 장치에 상기 어레이를 전기적으로 접속하는 수단을 포함한다. 샘플은 공통 로딩 채널로부터 상기 어레이내로 로딩되고, 웰에서 처리되며, 상기 광학 시스템에 의한 측정이 수행된다. 상기 어레이는 병렬로 많은 샘플을 처리, 또는 많은 화합물을 분석할 수 있어 이런 처리를 위해 요구되는 시간을 감소시킨다.

Process for preparing polynucleotides

New and useful intermediate nucleotides bound to an inorganic polymer support, including the preparation thereof, and processes for the conversion to oligonucleotides which are especially useful for the synthesis of polynucleotides, particularly ribonucleic (RNA) and deoxyribonucleic acids (DNA).

Automated system for polynucleotide synthesis and purification

A method and system for polynucleotide synthesis are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure sequences caused by chain growth from extraneous support sites, and allows lower amounts of monomer reactants to be used to achieve equal or better coupling efficiencies as those achieveable with CPG. The method and system also employ nucleoside intermediates whose exocyclic amines are protected by base-labile groups which permit simultaneous cleavage and deprotection of the completed polynucleotide chain in the presence of the solid phase support. This latter feature allows practical automation of both the synthesis and purification of polynucleotides.

Automated system for polynucleotide synthesis and purification

A method and system for polynucleotide synthesis and purification are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure squences caused by chain growth from extraneous support sites. Consequently, currently used rapid purification techniques depending on trityl hydrophobicity give a more highly purified product. The method and system also employ nucleoside intermediates whose exocyclic amines are protected by base-labile groups which permit simultaneous cleavage and deprotection of the completed polynucleotide chain in the presence of the solid phase support. This latter feature allows practical automation of both the synthesis and purification of polynucleotides.

Partitioned microelectronic device array

A system for processing a plurality of tests or syntheses in parallel comprising a sample channel for moving samples into a microlaboratory array of a plurality of wells connected by one or more channels for the testing or synthesis of samples, a station for housing the array and an optical system comprising at least one light source and at least one light detector for measuring the samples in the array, and a means of electrically connecting said array to an apparatus capable of monitoring and controlling the flow of fluids into the array. Samples are loaded from a common loading channel into the array, processed in the wells and measurements taken by the optical system. The array can process many samples, or synthesize many compounds in parallel, reducing the time required for such processes.

Microfluidic devices and methods for chemical assays

An assay device in which to carry out a fluid-phase chemical assay, comprising means for supporting a test substrate, a sample chamber for receiving a fluid sample and at least one fluid control device for controlling the movement of fluid into and/or out of the sample chamber, wherein the fluid control device comprises a fluid outlet chamber in fluid communication with the sample chamber, and a displaceable flexible diaphragm the displacement of which alters the volume of the outlet chamber so as to allow and/or restrict fluid flow between the outlet and sample chambers. The invention also provides assay apparatus incorporating such a device, an assay station for use as part of such apparatus, a fluid control unit for use as part of the assay device and a method of conducting an assay which may involve the use of such apparatus and devices.

Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis

A system for processing a plurality of tests or syntheses in parallel comprising a sample channel for moving samples into a microlaboratory array of a plurality of wells connected by one or more channels for the testing or synthesis of samples, a station for housing the array and an optical system comprising at least one light source and at least one light detector for measuring the samples in the array, and a means of electrically connecting the array to an apparatus capable of monitoring and controlling the flow of fluids into the array. Samples are loaded from a common loading channel into the array, processed in the wells and measurements taken by the optical system. The array can process many samples, or synthesize many compounds in parallel, reducing the time required for such processes.

Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof

The invention features methods of making devices, or 'platens' having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analizying the contents of the array, including assaying of physical properties of the samples. In various embodiments, the reagents can be contained within the through-holes by capillary action, attached to the walls of the through-hole. The porous material can be, for example, a gel, a bead, sintered glass, or particulate matter, or can be the inner wall of a ghrough-hole that has been chemically etched. In particular embodiments, the arrays can include invidual molecules, complexes of molecules, viruses, cells, groups of cells, pieces of tissue, or small particles or beads. The members of the arrays can aslo, for example, function as transducers that report the presence of an analyte (e.g., by providing an easily detected signal), or they can function as selective binding agents for the retention of analytes of interest. Using these methods, arrays corresponding to a large plurality of human genes (e.g., using nucleic acid probes) can also be prepared.

Liquid distribution system

The present invention provides a liquid distribution system, which is useful in a number of contexts, including in accomplishing various synthetic, diagnostic and drug screening reactions. The distribution system can comprise an alpha reservoir (200A) and a beta reservoir (200B), a first set of parallel and adjacent first and second feeder channels (211A-211B) and a second set of parallel and adjacent third and fourth feeder channels (211C-211D) which are offset from the first and second feed channels (211A-211B), wherein (a) the first (211A) and third (211C) feeder channels are connected to the alpha reservoir (200A) via a first connector channel (211A) that is situated above or below the second (211B) and fourth feeder channels (211D) and are independent of the beta reservoir and (b) the second (211B) and fourth feeder channels (211D) are connected to the beta reservoir via a second connector channel (211B) that it situated above or below the first and third feeder channels (211A and 211C) and are independent of the alpha reservoir (200A). The distribution system is preferably a microscale distribution system. Various particular mechanisms for controlling flow into a liquid distribution system are described.

Microfluidic reaction carrier

Gegenstand der vorliegenden Erfindung ist ein mikrofluidischer Reaktionsträger, der eine rein fluidische oder auch eine lichtgesteuerte Synthese und Analyse von Oligomeren oder Polymeren ermöglicht. The present invention relates to a microfluidic reaction carrier which enables purely fluidic or light-controlled synthesis and analysis of oligomers or polymers.

Integrated analysis system

Method for preparing positive electrode active material precursor for lithium secondary battery

본 발명은 적어도 2개 이상의 여과 장치가 배치(batch)식 반응기 외부에 설치된 제조 장치를 이용하여 리튬 이차전지용 양극 활물질 전구체를 제조하는 방법에 있어서, 상기 배치식 반응기에 전이금속 양이온을 포함하는 전이금속 함유 용액, 암모늄 이온 함유 용액 및 염기성 수용액을 포함하는 반응 용액을 연속적으로 투입하는 단계; 상기 배치식 반응기가 만액이 되면 상기 반응기 내의 슬러리를 상기 반응기 외부에 설치된 제1 여과 장치로 투입하는 단계; 상기 제1 여과 장치에 투입된 슬러리는, 필터부를 통과한 반응 모액 및 필터부를 통과하지 못한 농축 슬러리로 분리되며, 이 중 상기 반응 모액은 백 워싱 탱크(back washing tank)로 투입하고, 상기 농축 슬러리는 백 워싱 탱크에 보관된 상기 반응 모액을 이용하여 상기 배치식 반응기로 재투입하는 단계; 및, 상기 배치식 반응기로 재투입한 농축 슬러리는 입자 성장을 거쳐 양극 활물질 전구체를 형성하는 단계;를 포함하는, 양극 활물질 전구체의 제조 방법을 제공한다. The present invention relates to a method for producing a cathode active material precursor for a lithium secondary battery using a production device in which at least two or more filtration devices are installed outside the batch-type reactor, wherein the transition metal containing a transition metal cation in the batch-type reactor continuously introducing a reaction solution including the solution containing solution, the solution containing ammonium ions, and the basic aqueous solution; When the batch-type reactor is full, adding the slurry in the reactor to a first filtration device installed outside the reactor; The slurry introduced into the first filtration device is separated into a reaction mother liquid that has passed through the filter unit and a concentrated slurry that has not passed through the filter unit, of which the reaction mother liquid is introduced into a back washing tank, and the concentrated slurry is reintroducing the reaction mother liquid stored in a back washing tank into the batch reactor; And, the concentrated slurry reintroduced into the batch-type reactor to form a cathode active material precursor through particle growth; provides a method for producing a cathode active material precursor comprising a.

Micro-fluidic heating system

Provided is a micro-fluidic heating system, which comprises a micro-fluidic control element for providing a chamber, a flow path and a valve, and a main body for heating the inside of the chamber in contact with the micro-fluidic control element, wherein the micro-fluidic control element consists of an upper substrate for providing the chamber, the flow path and the valve, and a lower substrate as a thin film bonded to the upper substrate, and the main body consists of a membrane in which heating means and suction holes are formed, and support member for supporting the membrane, and the heating means is partially in contact with the lower substrate of the chamber to heat the inside of the chamber, so that thermal transfer efficiency becomes maximized and temperature of each chamber may be independently controlled in the case of configuration having chambers arranged in array.

Fluidics system for continuous transport of reagents

System for recovering double heat exchanging energy of hydrothermal carbonization and method therefor

수열탄화공정의 직간접 이중열교환 에너지 회수시스템 및 회수방법이 소개된다. 이 중에서 에너지 회수시스템은 유기성폐기물이 저장 가능하고 회수열을 이용하여 유기성폐기물을 기 설정된 예열 온도로 예열하는 저장조와, 저장조에서 예열된 유기성폐기물을 공급받아 기 설정된 수열반응온도로 가열하여 수열탄화 반응물을 생성하는 수열탄화 반응기와, 수열탄화 반응기로부터 공급받은 수열탄화 반응물로부터 열을 회수하는 수열탄화 열회수조와, 수열탄화 열회수조에서 공급받은 수열탄화 반응물 내 질소를 탈기하는 질소회수 반응기를 포함하고, 수열탄화 열회수조는 수열탄화 반응물로부터 회수된 일부 열을 회수열로서 저장조에 제공하고, 수열탄화 열회수조로부터 회수된 일부 열을 질소회수를 위한 반응열로서 질소회수 반응기에 제공할 수 있다. A direct and indirect double heat exchange energy recovery system and recovery method of the hydrothermal carbonization process are introduced. Among them, the energy recovery system includes a storage tank that can store organic waste and preheats the organic waste to a preset preheating temperature using recovered heat, and a hydrothermal carbonization reaction product by receiving the organic waste preheated from the storage tank and heating it to a preset hydrothermal reaction temperature. A hydrothermal carbonization reactor generating The carbonization heat recovery tank provides some heat recovered from the hydrothermal carbonization reactant to the storage tank as recovery heat, and the partial heat recovered from the hydrothermal carbonization heat recovery tank may be provided to the nitrogen recovery reactor as reaction heat for nitrogen recovery.

Liquid distribution system

본 발명은 여러 가지 합성, 진단 및 약품 스크리닝 반응을 포함하는 다수의 내용물에 유용한 액체 분배 시스템을 제공한다. 상기 분배 시스템은 알파 저장소(200A)와 베타 저장소(200B), 제 1 세트의 병렬 및 인접한 제 2 보급기 채널(211A-211B)과 상기 제1 및 제 2 보급 채널(211A-211B)로부터 오프셋되는 제 2 세트의 병렬 및 인접한 제 3 및 제 4 보급기 채널(211C-211D)을 포함할 수 있는데, (a) 상기 제 1(211A) 및 제 3(211C) 보급기 채널은 상기 제 2(211B) 및 제 4 보급기 채널(211D)의 상부 또는 하부에 배치되고 상기 베타 저장소와 독립적인 제 1 접속기 채널(211A)을 통해 상기 알파 저장소(200A)에 접속되고, (b) 상기 제 2(211B) 및 제 4 보급기 채널(211D)은 제 1 및 제 3 보급기 채널(211A와 211C)의 상부와 하부에 배치되고 알파 저장소(200A)와 독립적인 제 2 접속기 채널(211B)을 통해 베타 저장소에 접속된다. 상기 분배 시스템은 바람직하게 마이크로 크기 분배 시스템이다. 액체 분배 시스템내의 흐름을 제어하기 위한 여러 가지 특별한 매커니즘이 기술된다. The present invention provides a liquid dispensing system useful for a number of contents including various synthetic, diagnostic, and drug screening reactions. The distribution system is offset from the alpha reservoir 200A and the beta reservoir 200B, the first set of parallel and adjacent second feeder channels 211A-211B and the first and second feed channels 211A-211B. Two sets of parallel and adjacent third and fourth feeder channels 211C-211D, wherein (a) the first 211A and third 211C feeder channels comprise the second 211B and the second 4 is connected to the alpha reservoir 200A via a first connector channel 211A disposed above or below the diffuser channel 211D and independent of the beta reservoir, (b) the second 211B and fourth The diffuser channel 211D is connected to the beta reservoir via a second connector channel 211B disposed above and below the first and third diffuser channels 211A and 211C and independent of the alpha reservoir 200A. The dispensing system is preferably a micro size dispensing system. Several special mechanisms for controlling the flow in a liquid distribution system are described.

Methods and devices for high fidelity polynucleotide synthesis

Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.

Very large scale immobilized polymer synthesis using mechanically directed flow paths

A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are flowed through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

Nucleosides useful in the preparation of polynucleotides

New and useful intermediate nucleotides bound to an inorganic polymer support, including the preparation thereof, and processes for the conversion to oligonucleotides which are especially useful for the synthesis of polynucleotides, particularly ribonucleic (RNA) and deoxyribonucleic acids (DNA).

Process for preparing polynucleotides

This invention is a process for the production of oligonucleotides which comprises: (a) converting an inorganic polymer into a coupling agent-polymer; (b) combining the coupling agent-polymer with a blocked nucleoside to obtain a blocked nucleoside-modified support; and (c) removing the blocking group from the blocked nucleoside-modified support to provide for the coupling of nucleotides to the nucleoside-modified support.

Parallel flow reactor having variable composition

Parallel flow reaction systems comprising four or more reaction channels are disclosed. Distribution systems, and parallel flow reaction systems comprising such distribution systems are also disclosed. Specifically, the distribution systems comprise a feed-composition subsystem for providing a different feed composition to each of the four or more reactors. In preferred embodiments, the feed composition subsystem comprises at least one set of four or more feed-component flow restrictors, each of the four or more feed-component flow restrictors having a flow resistance that varies relative to other flow restrictors in the set.

Methods for identifying and optimizing materials in microfluidic systems

A chemical processing microsystem useful for identifying and optimizing materials (e.g., catalysts) that enhance chemical processes or for characterizing and/or optimizing chemical processes is disclosed. The chemical processing microsystem comprises a plurality of microreactors 600 and, in a preferred embodiment, a plurality of microseparators 900 integral with the chemical processing microsystem 10 . The microreactors 600 are preferably diffusion-mixed microreactors formed in a plurality of laminae that include a modular, interchangeable candidate-material array 100 . The material array 100 comprises a plurality of different candidate materials (e.g., catalysts), preferably arranged at separate, individually addressable portions of a substrate (e.g., wafer). The microseparators 900 are similarly formed in a plurality of laminae that include a modular, interchangeable adsorbent array 700 . The adsorbent array 700 comprises one or more adsorbents, preferably arranged at separate, individually addressable portions of a substrate to spatially correspond to the plurality of different candidate materials. Modular microfluidic distribution systems are also disclosed. The chemical processing microsystem can be integrated into a material evaluation system that enables a comprehensive combinatorial material science research program.

Chemical processing microsystems comprising parallel flow microreactors and methods for using same

A chemical processing microsystem useful for identifying and optimizing materials (e.g., catalysts) that enhance chemical processes or for characterizing and/or optimizing chemical processes is disclosed. The chemical processing microsystem comprises a plurality of microreactors 600 and, in a preferred embodiment, a plurality of microseparators 900 integral with the chemical processing microsystem 10 . The microreactors 600 are preferably diffusion-mixed microreactors formed in a plurality of laminae that include a modular, interchangeable candidate-material array 100 . The material array 100 comprises a plurality of different candidate materials (e.g., catalysts), preferably arranged at separate, individually addressable portions of a substrate (e.g., wafer). The microseparators 900 are similarly formed in a plurality of laminae that include a modular, interchangeable adsorbent array 700 . The adsorbent array 700 comprises one or more adsorbents, preferably arranged at separate, individually, addressable portions of a substrate to spatially correspond to the plurality of different candidate materials. Modular microfluidic distribution systems are also disclosed. The chemical processing microsystem can be integrated into a material evaluation system that enables a comprehensive combinatorial material science research program.

Automation type powder washing device system for synthesis of high furity powder

자동화 방식의 도입으로 ZnS 나노분말 특성에 대한 균일성을 향상시킬 수 있는 고순도 분말 합성을 위한 자동화 방식의 분말 수세 장치 시스템에 대하여 개시한다. 본 발명에 따른 고순도 분말 합성을 위한 자동화 방식의 분말 수세 장치 시스템은 반응조; 상기 반응조에 ZnS 나노분말 슬러리를 공급하기 위한 슬러리 저장 탱크; 상기 반응조에 수세액을 공급하기 위한 수세액 저장 탱크; 상기 반응조의 내부로 공급되는 ZnS 나노분말 슬러리를 수세액으로 교반하면서 수세하기 위한 오버 헤드 스터러; 상기 반응조의 내부에 장착되며, 수세된 상기 ZnS 나노분말 슬러리를 여과하기 위한 여과 필터; 상기 여과 필터에 의해 필터링된 상기 ZnS 나노분말 슬러리를 건조하기 위해, 상기 반응조의 외부에 장착된 히팅 유닛; 및 상기 반응조의 하측에 장착되어, 상기 여과 필터를 통과한 여과액이 배출되는 여과액 저장 탱크;를 포함하는 것을 특징으로 한다. Disclosed is a powder washing device system of an automated method for synthesizing high-purity powders that can improve the uniformity of ZnS nanopowder properties by introducing an automated method. An automated powder washing device system for synthesizing high-purity powder according to the present invention comprises: a reaction tank; A slurry storage tank for supplying the ZnS nanopowder slurry to the reaction tank; A washing liquid storage tank for supplying a washing liquid to the reaction tank; An overhead stirrer for washing with water while stirring the ZnS nanopowder slurry supplied to the inside of the reaction tank with a water washing solution; A filtration filter mounted inside the reaction tank and configured to filter the washed ZnS nanopowder slurry; A heating unit mounted on the outside of the reaction tank to dry the ZnS nanopowder slurry filtered by the filtration filter; And a filtrate storage tank mounted below the reaction tank to discharge the filtrate that has passed through the filtration filter.

Microfluidic device for the generation of combinatorial samples

Un dispositivo microfluídico para producir gotitas de al menos una muestra en una fase inmiscible, comprendiendo el dispositivo un formador de gotitas (100) que conecta un canal de fase inmiscible (42) y un canal de muestra que tiene una pluralidad de entradas de muestra (25) conectadas a una pluralidad de canales de entrada de muestra (27) que inyectan la al menos una muestra en el canal de muestra (29), en donde la inyección de la al menos una muestra se controla mediante una pluralidad de válvulas de muestra (20), de manera que la al menos una muestra fluye o bien hacia una salida de residuos de muestra (30) o hacia un canal de la pluralidad de canales de entrada de muestra (27), en donde la pluralidad de canales de entrada de muestra (27) tienen la misma resistencia hidrodinámica resultante de la longitud, la altura y la anchura del canal de entrada de muestra (27) corriente arriba del formador de gotitas (100), el dispositivo microfluídico tiene un canal de salida (60) corriente abajo del formador de gotitas (100), en el que las gotitas fluyen hacia dicho canal de salida, caracterizado por que la resistencia hidrodinámica del canal de salida (60) es menor que la resistencia hidrodinámica del canal de fase inmiscible (42), y en donde dicha resistencia hidrodinámica del canal de salida resultante de la longitud, la altura y la anchura del canal de salida es menor que la resistencia hidrodinámica de los canales de entrada de muestra. A microfluidic device for producing droplets of at least one sample in an immiscible phase, the device comprising a droplet former (100) connecting an immiscible phase channel (42) and a sample channel having a plurality of sample inlets ( 25) connected to a plurality of sample inlet channels (27) that inject the at least one sample into the sample channel (29), wherein the injection of the at least one sample is controlled by a plurality of sample valves (20), such that the at least one sample flows either into a sample waste outlet ...

Device for examining a biological sample

Fluidisches Netzwerk (1) zur parallelen Durchführung einer Vielzahl von Untersuchungen von biologischen Proben aufweisend ein Durchflussarray (2) mit einer Vielzahl von Reaktionsräumen (14), wobei die Reaktionsräume (14) jeweils einen ersten Kanalanschluss (20) und einen zweiten Kanalanschluss (21) aufweisen, wobei die ersten Kanalanschlüsse (20) an einen ersten Versorgungskanal (16) angeschlossen sind und die zweiten Kanalanschlüsse (21) an einen zweiten Versorgungskanal (19) angeschlossen sind, wobei der erste Versorgungskanal (16) und der zweite Kanalanschluss (19) mit einer Zirkulationsleitung (6) miteinander verbunden sind, wobei an der Zirkulationsleitung mindestens eine Komponente (3, 4, 5, 7) angeschlossen ist, mit welcher Versuchsreagenzien in die Reaktionsräume (14) des Durchflussarrays (2) einbringbar sind.

Reaction vessel agitation apparatus

A device and method for efficiently synthesizing diverse molecular products on substrates. A parent vessel 200 contains a suspension of substrates. The suspension is pressurized with argon and transferred to a plurality of reaction vessels 201-209 in one or more reaction vessel banks where monomer addition reactions take place. Optionally, the substrates may be tagged with a tag monomer. A vortexing motor 300 vortexes the contents of reaction vessels 201-209 during monomer addition reactions to enhance synthesis. After the desired monomer and/or tag monomer addition reaction, the suspension is pressurized with argon and transferred back to parent vessel 200 for mixing. Thereafter, the suspension may be pressurized with argon and reallocated among reaction vessels 201-209 for further synthesis.

Fluidics system for sequential delivery of reagents

The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion. The invention is particularly advantageous in apparatus for performing sensitive multistep reactions, such as pH-based DNA sequencing reactions.

Synthesizing and screening molecular diversity

有效地在底物上合成不同分子产物的设备和方法。一透明容器(200)含有底物的悬浮体。将底物用氩气加压并转移至发生单体反应的一个或多个反应容器库的多个反应容器中(201-209)。选择性地，底物可用一标记单体标记。在单体加成反应期间，用旋涡马达旋流反应容器(201-209)中的内含物以促进合成。在期望的单体和/或标记单体加成反应之后，将悬浮体用氩气加压，转移回到透明容器(200)中混合。此后，可将悬浮浮体用氩气加压，并被再分配在反应容器中(201-209)用于进一步的合成。

High throughput screening of crystallization materials

High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.

Microfluidic reaction apparatus for high throughput screening

An SBS-formatted microfluidic device where the geometry of the plate defines an array of interrogation areas, and where each interrogation area encompasses at least one reaction site.

Systems for filling a sample array by droplet dragging

Systems for filling sample array by droplet dragging are provided. One aspect of the invention provides an array filling system for filling a platen having a platen surface and an array of receptacles, the receptacles having an internal surface and the receptacles separated by the platen surface, the system comprising: a liquid transfer device capable of holding liquid; and a controller configured to position the liquid transfer device in proximity to the platen surface and to move the liquid transfer device across the surface and over the receptacles to be filled so as to cause sequential communication of liquid in the liquid transfer device with the interior surface of each receptacle.

Microstructures for use in biological assays and reactions

The present invention relates to microstructures which a fabricated from a cast or molded polymer material, such as polydimethylsiloxane (PDMS). The invention describes and claims methods and compositions from which a variety of microstructures may be prepared. The microstructures are particularly useful for use in assays and reactions in the relating to biological assays, including applications in medical diagnostics. Such assays and reactions included, but are by no means limited to, polynucleotide sequencing and polynucleotides analysis, including analysis of single nucleotide polymorphisms, and for polynucleotide amplification such as PCR. The microstructures of the invention may contain many features that are useful for such applications. For example, the microstructures may comprise wells or channels for biological fluids and/or reagents, as well as pumps and valves for directing such fluids, e.g., in a biological assay. The microstructures may also comprise a variety of optical devices, including lenses, mirrors, optical waveguides, diffractions gratings, and optical absorbers and/or scatters such as “quantum dots.”

Crystal growth devices and systems, and methods for using same

High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.

Apparatus for substrate handling

Devices, apparatus and methods are disclosed for carrying out processing steps involved in chemical reactions such as hybridization reactions conducted on the surface of a substrate comprising chemical compounds such as biopolymer features. A device comprises a housing comprising a housing chamber. The housing comprises an opening through which a substrate may be inserted into the housing chamber. The device may also comprise a lift mechanism for moving the substrate in and out of the housing chamber in a controlled manner. At least one inlet may be in fluid communication with the housing chamber and at least one outlet may be in fluid communication with the housing chamber. Also disclosed are methods wherein the surface is brought into contact with a processing fluid; and, then, the surface is removed from contact with the fluid in a controlled manner at a rate that substantially eliminates droplet formation of the fluid on the surface of the substrate.

High throughput screening of crystallization of materials

High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.

Miniaturized cell array methods and apparatus for cell-based screening

The present invention describes novel methods for making a substrate for selective cell patterning, and the substrates themselves, wherein the method comprises contacting reactive hydroxyl groups on the surface of a substrate with a hydroxyl-reactive bifunctional molecule to form a monolayer, and using stencils to deposit cell repulsive or cell adhesive moieties in controlled locations on the cell culture substrate. Methods comprising selective differentiation of stem cells to create tissue specific and organ-specific cell substrates, as well as the cell substrates themselves are also provided.

Multiple fluid sample processor and system

Multiple fluid sample processors and systems for high throughput chemical synthesis and biological assays and/or processing. A multi-layered fluidic array having microchannels, reservoirs and reaction wells is subject to robotic and automated handling. A pressure pumping system is utilized for fluid delivery and control through the synthesis process. The sizes of the micro-sized channels, apertures, and valves are adjusted to optimize fluid distribution and channel filling. The fluid sample processors can be grouped together in a microtiter format to increase the speed, quantity and efficiency of the processing.

Systems and methods for mixing reactants

High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.

Microfluidic reaction apparatus for high throughput screening

A microfluidic device is described that has a slug mixing arrangement. A reaction well has an input flow channel with a first valve near the reaction well, and a second valve further from the reaction well. A fluid source is connected to the segment in between the two valves. A second fluid source is connected behind the second valve. The channel between the two valves receives the first fluid by blind filling when the two valves are closed. The reaction well receives the first fluid followed by the second fluid when the first and second valves are open.

Linear valve arrays

The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.

Linear valve arrays

The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.