УСТРОЙСТВО ДЛЯ ЭКСТРАКЦИИ БИОМОЛЕКУЛ И СПОСОБ ЭКСТРАКЦИИ БИОМОЛЕКУЛ

Группа изобретений относится к области биохимии. Предложен способ и устройство для экстракции биомолекул. Устройство содержит вставной элемент, корпусный элемент с лизирующим буфером и выводной элемент для вывода биомолекул. При этом вставной элемент служит для прикрепления образца, где вставной элемент содержит крепежную деталь. Способ включает прикрепление содержащего отобранный биоматериал образца к вставному элементу, введение вставного элемента в корпусный элемент с лизирующим буфером и вывод экстрагированных из биоматериала биомолекул через выводной элемент. Изобретения обеспечивают упрощение процесса экстракции биомолекул из биоматериала, возможность эффективно и беспрепятственно выполнять тестирование с получением достоверных результатов тестирования за счет минимизации вреда от экстрагированной биомолекулы и экстракции с высокой концентрацией при небольшом количестве образцов. 2 н. и 14 з.п. ф-лы, 14 ил., 1 табл.

Sample collection device

A sampling device having a lower portion with a sample container. A cap is moveably attached with the lower portion and includes a cutting edge configured for cutting a leaf. When the cap is attached to the lower portion with a leaf there between, a leaf sample is deposited into the sample container of the lower portion. The cap includes a vent in fluid communication with the sample container such that the leaf sample is dried. A detachable label can extend from the lower portion.

BIOMOLECULE EXTRACTION DEVICE AND BIOMOLECULE EXTRACTION METHOD

Disclosed are a biomolecule extraction device and a biomolecule extraction method. The biomolecule extraction device and the biomolecule extraction method according to the present invention make it possible to simplify the process for extracting biomolecules such as protein and nucleic acid from a biomaterial such as tissue and cells and make it possible to reduce the time and space needed for the extraction process, thereby improving user convenience. Also, dead space may be minimized to increase the extracted biomolecule concentration, and the usage of a buffer may be reduced. Also, extracted samples may be constantly discharged to perform testing. In addition, it is unnecessary to use a separate device or tool, and damage to extracted biomolecules is minimized. Also, consistent test results may be expected even when a small amount of a sample is applied.

FLASK FOR REAGENTS WITH SUCTION TUBE OF

MICROFLUIDICS SORTER FOR CELL DETECTION AND ISOLATION

An interface comprising: a plurality of external ports configured to fluidically communicate with a plurality of ports of a fluidic delivery platform; and a plurality of engaging conduits configured to fluidically communicate with a plurality of ports of a microfluidic biochip, wherein a tolerance of both the plurality of external ports and/or the plurality of engaging conduits is significantly tighter than a tolerance of the plurality of ports of the microfluidic biochip.

BIOSENSOR AND BIOSENSOR MANUFACTURING METHOD

A biosensor manufacturing method including a sheet material forming process and a dicing process. In the sheet material forming process a sheet material with plural biosensor forming sections is formed. Each of the biosensor forming sections includes a first base plate, a second base plate stacked on the first base plate and forming a capillary between the second base plate and the leading end portion of the first base plate for sucking in sample liquid, and a hydrophilic layer formed on the second base plate at least in a region facing the capillary. In the dicing process plural biosensors are obtained by dicing the sheet material with a blade from the first base plate side at the leading end of each of the biosensor forming sections, such that the leading end of the capillary opens onto the leading end face of the first base plate and the second base plate.

УСТРОЙСТВО ДЛЯ ЭКСТРАКЦИИ БИОМОЛЕКУЛ И СПОСОБ ЭКСТРАКЦИИ БИОМОЛЕКУЛ

Improved collection and storage apparatus

This invention relates to an evidence collection and storage apparatus for use in forensic examination and sample collection processes. The apparatus consists of a container and a swab, the swab including a handle portion, shaft portion and a removable head portion, wherein the container includes a cavity adapted to store an object or substance, a lid including a portion of nanomesh and a swab head portion removing means that engages the head portion of the swab within the cavity, enabling the head portion of the swab to be removed or partially removed from the shaft portion of the swab and stored in the container.

SYSTEMS AND METHODS FOR INTEGRATION OF MICROFLUIDIC TEAR COLLECTION AND LATERAL FLOW ANALYSIS OF ANALYTES OF INTEREST

Systems, methods, and devices for analyzing small volumes of fluidic samples, as a non-limiting example, less than twenty microliters are provided. The devices are configured to make a first sample reading, for example, measure an energy property of the fluid sample, for example, osmolality, make a second sample reading, for example, detecting the presence or concentration of one or more analytes in the fluid sample, or make both the first sample reading and the second sample reading, for example, measuring the energy property of the fluid sample as well as detecting the presence or concentration of one or more analytes in the fluid sample.

IMPROVED CONTROL BUTTON FOR A SAMPLING PIPETTE

The invention relates to a control button (12) for a manually actuated sampling pipette, the button comprising lower and upper parts (24a, 24b), one of which is equipped with a force sensor (31) and the other with an actuating member (30). In the vertical position of the button, in a state not subjected to a force, not only does the member (30) ensure that the upper part is retained axially on the lower part (24a), but these parts also define a circular axially retaining connection (42) therebetween, the circular connection exhibiting an axial clearance (47) configured such that, when a pressure is exerted on an off-centre action zone (102a) by the thumb of an operator, the axial clearance (47) is taken up in this zone (102a), while the connection (42) retains a reaction zone (102b), arranged diametrically opposite the action zone (102a), axially and locally relative to the lower part (24a).

Collector architecture layout design

The disclosure provides for compositions and methods for the collection of rare cells using an interspersed microstructure design.

Systems And Methods For Simultaneous Detection And Identification Of Microorganisms Within A Fluid Sample

An optical measurement instrument is an integrated instrument that includes an optical cavity with a light source, a sample cuvette, and an optical sensor. The light source and sensor are on a bench that is on a translational or rotational mechanical platform such that optical beam can be moved to multiple sample containers. Each sample containers holds a distinct microorganism-attracting substance and a portion of a fluid sample containing an unknown microorganism. Each distinct microorganism-attracting substance is configured to bind with a single type of microorganism. The unknown microorganism in the fluid sample binds with the distinct microorganism-attracting substance in a single sample container. The instrument incubates the microorganism in the single sample container and detects the presence of the microorganism in the single sample container to thereby simultaneously identify the unknown microorganism.

METHODS AND APPLICATIONS OF ON-CHIP DRIED OR LYOPHILIZED CHEMILUMINESCENCE SUBSTRATE REAGENTS

A sensing device includes a sample loading chamber configured to receive a sample, a detection antibody drying or lyophilization chamber configured to receive a first portion of the sample, one or more substrate drying or lyophilization chambers configured to receive a second portion of the sample, and one or more reaction chambers connected to the detection antibody drying or lyophilization chamber and the one or more substrate drying or lyophilization chambers. The detection antibody drying or lyophilization chamber and one or more substrate drying or lyophilization chambers are placed in parallel between the sample loading chamber and the one or more reaction chambers ...

СПОСОБ ИЗГОТОВЛЕНИЯ ЧИПА ДЛЯ АНАЛИЗА И ЧИП ДЛЯ АНАЛИЗА

Группа изобретений относится к области биологических анализов. Раскрыт способ изготовления чипа для анализа биологических образцов, включающий обеспечение матрицы (10), обеспечение, по меньшей мере, одного вкладыша (3), вставку, по меньшей мере, одного вкладыша (3), по меньшей мере, в одно сквозное отверстие (11) матрицы (10) посредством поступательного перемещения, по меньшей мере, одного вкладыша (3) в направлении, нормальном к нижней и верхней поверхностям матрицы (10), осуществление механической сборки при температуре ниже температур плавления материала носителя и материала для анализа, в ходе которой прикладывают прижимающее усилие, причем указанная механическая сборка, по меньшей мере, одного вкладыша (3) с матрицей (10) приводит к обжатию, по меньшей мере, одного вкладыша (3) матрицей (10) на, по меньшей мере, части его нижней и верхней поверхностей. Также раскрыты чип (1) для анализа биологических образцов и его применение для обнаружения сложных биомолекул в биологических средах ...

Biomolecule extraction device and biomolecule extraction method

Disclosed are a biomolecule extraction device and a biomolecule extraction method. The biomolecule extraction device and the biomolecule extraction method according to the present invention make it possible to simplify the process for extracting biomolecules such as protein and nucleic acid from a biomaterial such as tissue and cells and make it possible to reduce the time and space needed for the extraction process, thereby improving user convenience. Also, dead space may be minimized to increase the extracted biomolecule concentration, and the usage of a buffer may be reduced. Also, extracted samples may be constantly discharged to perform testing. In addition, it is unnecessary to use a separate device or tool, and damage to extracted biomolecules is minimized. Also, consistent test results may be expected even when a small amount of a sample is applied.

Apparatus and method for freezing specimens

The present invention relates to an apparatus for freezing samples such as tissue specimens, particularly for rapidly freezing multiple specimens without the need to sequentially prepare and freeze each sample individually. The apparatus comprises a mold which can accommodate one or more tissue samples, a hands-free means for supporting the mold, an insulated container for holding a cryogenic material or refrigerant, and an optional cover for the insulated container. The invention also relates to methods for freezing the tissue specimens.

Whole blood separation sampling apparatus

The present invention provides systems, devices, kits, and methods for separating blood plasma or serum from whole blood. The present invention further provides systems, devices, and methods for separating a volume of blood plasma or serum from whole blood.

Microfluidic system for intracellular delivery of substances and method therefor

Provided is a microfluidic system for delivering external substances into cells by cell perforation performed using inertia, the system comprising a fluid channel structure through which a solution containing cells and external substances continuously flows, wherein the fluid channel structure includes a junction between one or more channels, a local vortex occurs near the interface of the junction, the cells are deformed by the vortex, the vortex causes a temporary discontinuity in the cell membrane, and the external substances are introduced into the cells by solution exchange between the cells and the fluid around the cells.

SELECTIVE DELIVERY OF MATERIAL TO CELLS

Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.

A CHEMICAL SENSOR

CENTRIFUGAL FORCE-BASED MICROFLUIDIC DEVICE, CAPABLE OF PREVENTING RELIABILITY DEGRADATION OF A BIOCHEMICAL TEST WITHOUT INCLUDING A CLAMP, A BIOCHEMICAL TREATMENT SYSTEM INCLUDING THE SAME, AND A METHOD FOR MANUFACTURING THE SAME

PURPOSE: A centrifugal force-based microflluidic device is provided to be stably fixed to a turntable, to reduce a domain required for fixing the microfluidic device to the turntable, and to prevent reliability degradation of a biochemical test without including a clamp. CONSTITUTION: A centrifugal force-based microfluidic device(10) comprises a rotatable platform(12) and a cap(21). The rotatable platform includes a unit for biochemically treating a sample and a mounting through-hole(13) in which a turntable with a magnetic force generating unit is fitted. The cap is combined with the rotatable plarform to close an opposite side from the side in which the mounting through-hole is inserted. The cap includes partially a ferromagnetic substance. COPYRIGHT KIPO 2011 ...

МИКРОЖИДКОСТНОЕ УСТРОЙСТВО ДЛЯ СЕЛЕКТИВНОГО ЗАХВАТА БИОЛОГИЧЕСКИХ ОБЪЕКТОВ

Изобретение относится к микрожидкостным устройствам. Раскрыто микрожидкостное устройство для селективного захвата целевых биологических объектов, содержащее а) массив симметричных столбиков в форме капли (1), в которых линии хорды (5) симметричных столбиков в форме капли параллельны друг другу и при этом геометрические центры симметричных столбиков в форме капли образуют ромбическую решетку (4), характеризующуюся тем, что длина (d) стороны ромба в решетке образована как Евклидово расстояние между геометрическими центрами двух соседних симметричных столбиков в форме капли, b) извилистый микрожидкостный канал (2) и c) объем захвата (3), при этом извилистый микрожидкостный канал (2) содержит последовательность сегментов эллипса (13), каждый из которых характеризуется хордой, параллельной большой или малой оси эллипса, причем указанная хорда разделена на секцию впуска (17) и секцию выпуска (18), через которые целевой биологический объект входит в сегмент эллипса (13) и выходит из него, причем ...

Устройство и способ для замораживания образцов

Selective delivery of material to cells

Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell. WO 2015/023982 PCT/US2014/051343 Protocol 1. Lyse red blood cells 2. Deliver dye via CellSqueeze o o o °oo o o o o 3. Sort cells Fluorescent Label + & CD45/- O Fluorescent Label - or CD45 + 0*0 CIIICTITIITr: CI-I :T IDIII r: )9 ...

Reagent bottle with aspiration pipe

An embodiment of a reagent container includes a bottle with a pipe to reduce the effects of reagent sloshing. The bottle has an elongated base and an opposed cover connected by side walls and an end wall. A flat platform surrounded by a raised rim lies in the base opposite an opening in the cover. A ribbed pipe frictionally fits within the bottle opening and may attach to the anchor region leaving vent passages around the pipe. The pipe includes an aperture adjacent to the anchor region and oriented toward the end wall so that sloshed fluid has only a small effect on the level of reagent in the pipe during transfers. A modified blow molding process produces the anchor region by extending a pin a predetermined distance into a mold while the molded material is still plastic.

Selective delivery of material to cells

Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.

BIOLOGICAL SAMPLING AND STORAGE CONTAINER

The invention relates to a biological sampling and storage container comprising a top cover and a bottom cover adapted to fit onto each other; and a sampling element having a proximal end onto which the top cover fits and a distal end onto which the bottom cover fits, said sampling element further comprising a sampling container comprising a sampling chamber for accepting the biological sample; an aliquoting element removably mounted on and in said sampling container, said aliquoting element comprising a structural element onto which one or more aliquoting chambers are positioned; and optionally, an ejector removably mounted onto said aliquoting element, wherein the ejector comprises an ejector wand fitting into the aliquoting chambers.

REAGENT BOTTLE WITH ASPIRATION PIPE

An embodiment of a reagent container includes a bottle with a pipe to reduce the effects of reagent sloshing. The bottle has an elongated base and an opposed cover connected by side walls and an end wall. A flat platform surrounded by a raised rim lies in the base opposite an opening in the cover. A ribbed pipe frictionally fits within the bottle opening and may attach to the anchor region leaving vent passages around the pipe. The pipe includes an aperture adjacent to the anchor region and oriented toward the end wall so that sloshed fluid has only a small effect on the level of reagent in the pipe during transfers. A modified blow molding process produces the anchor region by extending a pin a predetermined distance into a mold while the molded material is still plastic.

A MICROFLUIDIC CENTRIFUGE DEVICE AND METHOD FOR PERFORMING SOLUTION EXCHANGE AND SEPARATION

A microfluidic centrifuge device for solution exchange and separation. The device includes a curved fluidic channel, a first inlet and a second inlet in fluid communication with a proximal end of the curved fluid channel, and a first outlet and a second outlet in fluid communication with a distal end of the curved fluidic channel. The curved fluidic channel has dimensions sufficient to direct a first subset of the microparticles to the first outlet by Dean drag and a second subset of the microparticles to the second outlet by inertial force.

Biological chip and preparation method thereof

HEATING DEVICE FOR DETECTING POTENTIAL IMPACT TRACE

The present invention relates to a heating device for detecting a potential impact trace, comprises: a sample treated with an amino acid reaction reagent through an amino acid capable of reacting with a potential impact trace; a first heating plate formed in a plate shape, provided with a first steam hole capable of emitting steam on a lower surface thereof and heated at a predetermined temperature; a second heating plate formed in a plate shape, provided with a second steam hole capable of emitting steam on an upper surface thereof and heated at a predetermined temperature; and a connecting unit connecting the first heating plate and the second heating plate so that the first heating plate is rotatable with respect to the second heating plate. The sample is sandwiched between the first heating plate and the second heating plate, and is simultaneously heated from the first heating plate and the second heating plate. COPYRIGHT KIPO 2018 ...

ARTICLES AND SYSTEMS INVOLVING REACTION PRODUCTS ON SURFACES AND ASSOCIATED METHODS

Methods related to forming reaction products on surfaces are generally provided. Some methods comprise applying first and second polyelectrolytes in first and second polyelectrolyte carrier fluids to a surface. The first and second polyelectrolytes may be different, and the first and second carrier fluids may be the same or different. Some methods comprise forming a mixture of the first and second polyelectrolytes in a mixture carrier fluid that comprises the first and/or second carrier fluids. The first and second polyelectrolyte may be removed from the mixture carrier fluid to form a reaction product on the surface. In some embodiments, the mixture carrier fluid comprises a salt with a molecular weight of less than or equal to 1 kg/mol at a concentration within the mixture carrier fluid of from 0.01 M to 0.5 M. In some embodiments, the mixture carrier fluid has a turbidity of greater than or equal to 10 NTU and a viscosity of less than or equal to 1 Pa*s.

Visual Bionic Digestive System for Human Gastrointestinal Tract Model

The present invention discloses a visual bionic digestive system for a human gastrointestinal tract model, and belongs to the field of bionic digestive systems. The visual bionic digestive system for a human gastrointestinal tract model comprises a reaction system and a control system; the reaction system comprises a bionic stomach system, a bionic small intestine system, a bionic large intestine system and a filtering system; the control system comprises a PLC, peristaltic pumps, and a circulating water tank; the water inlet pipes of the bionic stomach system, the bionic small intestine system, and the bionic large intestine system are connected to a water outlet of the circulating water tank through water pipes; the water outlet pipes of the bionic stomach system, the bionic small intestine system, and the bionic large intestine system are connected to a water inlet of the circulating water tank through water pipes, solenoid valves are arranged on the water pipes, and the solenoid valves ...

Method for analyte examination

A method may include steps of binding an analyte to a ligand, exposing the analyte to light of differing wavelengths, and observing a difference in spectral response of the analyte at the differing wavelengths. Further steps may include comparing the observed difference with a known difference in spectral response of a reference substance at the differing wavelengths, and determining whether the observed difference meets a predetermined threshold of identity with the known difference. The method may be performed with reference to a surface having a reflective area with a known size a non-reflective area adjacent to the reflective area. The method may thus include the steps of providing a ligand in alignment with the non-reflective area of the surface, and binding an analyte to the ligand in a position reaching across the reflective area. Further steps may include forming an image of the analyte and the reflective area, observing the size of analyte relative to the reflective area in the ...

УСТРОЙСТВО ПРОБОПОДГОТОВКИ ДЛЯ ИММУНОФЕРМЕНТНЫХ ИССЛЕДОВАНИЙ

Изобретение относится к клинической лабораторной диагностике. Раскрыто устройство пробоподготовки для иммуноферментных исследований, выполненное в виде контейнера прямоугольной формы и состоящее из полки, которая содержит углубления в форме гнезд, при этом полка имеет 136 гнезд, содержащих по два несквозных ступенчатых сопряженных отверстия, диаметры которых между собой соотносятся в пропорции 12,1 к 7,7, при этом 96 гнезд расположены в 8-мь рядов, в каждом из которых 12 гнезд и которые расположены параллельно друг к другу, и перпендикулярно к узким сторонам контейнера в границах прямоугольного контура, за пределами которого расположены в 4 ряда 40 гнезд, причем каждый из 4-х рядов расположен параллельно сторонам прямоугольного контура, при этом 2 ряда, которые расположены параллельно длинной стороне прямоугольного контура, содержат по 12 гнезд, а 2 ряда, которые расположены параллельно короткой стороне прямоугольного контура, содержат по 8 гнезд. Изобретение обеспечивает надежную установку ...

미세 유체 눈물 포집 및 관심 대상인 분석물의 측방 유동 분석의 통합을 위한 시스템 및 방법

... 비제한적인 예로서 20 마이크로리터 미만인 소량의 유체 샘플을 분석하기 위한 시스템, 방법, 및 장치가 제공된다. 장치는 제1 샘플 판독값이, 예컨대 몰삼투압 농도와 같은 유체 샘플의 에너지 특성을 측정하게 하거나, 제2 샘플 판독값이, 예컨대 유체 샘플 내의 하나 이상의 분석물의 존재 또는 농도를 검출하게 하거나, 또는 제1 샘플 판독값과 제2 샘플 판독값 모두가, 예컨대 유체 샘플의 에너지 특성을 측정하는 것 뿐만 아니라 유체 샘플 내의 하나 이상의 분석물의 존재 또는 농도를 검출하는 것을 행하게 하도록 구성된다.

SAMPLE COLLECTION DEVICE

A sampling device having a lower portion with a sample container. A cap is moveably attached with the lower portion and includes a cutting edge configured for cutting a leaf. When the cap is attached to the lower portion with a leaf there between, a leaf sample is deposited into the sample container of the lower portion. The cap includes a vent in fluid communication with the sample container such that the leaf sample is dried. A detachable label can extend from the lower portion.

Non-invasive passive interstitial fluid collector

A microfluidic device for non-invasively and passively accessing interstitial fluid from a patient includes a substrate containing multiple vertical micro channels therethrough, wherein at a first end of each of the multiple vertical micro channels a microheater is formed for controllably ablating a portion of dry dead skin cells to access the interstitial fluid; and wherein at a second end of each of the multiple vertical micro channels is a horizontal micro channel for receiving accessed interstitial fluid from a vertical micro channel and guiding the accessed interstitial fluid to a common collection port.

MICROFLUIDIC SYSTEM FOR INTRACELLULAR DELIVERY OF MATERIALS AND METHOD THEREFOR

Provided is a microfluidic system for delivering external substances into cells by cell perforation performed using inertia, the system comprising a fluid channel structure through which a solution containing cells and external substances continuously flows, wherein the fluid channel structure includes a junction between one or more channels, a local vortex occurs near the interface of the junction, the cells are deformed by the vortex, the vortex causes a temporary discontinuity in the cell membrane, and the external substances are introduced into the cells by solution exchange between the cells and the fluid around the cells.

MICROFLUIDIC DEVICES AND SYSTEMS, AND METHODS FOR OPERATING MICROFLUIDIC DEVICES AND SYSTEMS

A microfluidic device includes a substrate having a first fluid inlet/outlet system, a second fluid inlet/outlet system, and a fluidic network between the first fluid inlet/outlet system and the second fluid inlet/outlet system and in fluid communication with the first fluid inlet/outlet system and the second fluid inlet/outlet system. The fluidic network includes a microfluidic channel network that is in fluid communication with the first fluid inlet/outlet system and spaced from the second fluid inlet/outlet system, a nanofluidic channel network fluidly connecting the microfluidic channel network and the second fluid inlet/outlet system, and a plurality of pores in fluid communication with the microfluidic channel network and the nanofluidic channel network.

APPARATUS AND METHOD FOR FREEZING SPECIMENS

BIOMOLECULE EXTRACTION DEVICE AND BIOMOLECULE EXTRACTION METHOD

Disclosed are a biomolecule extraction device and a biomolecule extraction method. The biomolecule extraction device and the biomolecule extraction method according to the present invention make it possible to simplify the process for extracting biomolecules such as protein and nucleic acid from a biomaterial such as tissue and cells and make it possible to reduce the time and space needed for the extraction process, thereby improving user convenience. Also, dead space may be minimized to increase the extracted biomolecule concentration, and the usage of a buffer may be reduced. Also, extracted samples may be constantly discharged to perform testing. In addition, it is unnecessary to use a separate device or tool, and damage to extracted biomolecules is minimized. Also, consistent test results may be expected even when a small amount of a sample is applied.

중합 효소 연쇄 반응을 실행하기 위한 분석 유닛, 분석 장치, 상기 분석 유닛의 작동 방법 및 상기 분석 유닛의 제조 방법

... 본 발명은 중합 효소 연쇄 반응을 실행하기 위한 분석 유닛(100)에 관한 것이며, 상기 분석 유닛(100)은 적어도 하나의 리드 리세스(135)를 가진 리드 부재(130)와 적어도 하나의 베이스 리세스(152)를 가진 베이스 부재(150)를 포함하고, 반응 챔버(110)를 형성하기 위해, 베이스 리세스(152)는 리드 리세스(135)에 대향 배치된다. 또한 분석 유닛(100)은 필름(140)을 포함하고, 상기 필름은 리드 부재(130)와 베이스 부재(150) 사이의 적어도 리드 리세스(135)의 영역에 배치된다. 또한 반응 챔버(110)의 베이스 리세스(152) 내로 유체를 유입 및/또는 베이스 리세스(152) 밖으로 배출하기 위해, 분석 유닛(100)은 리드 부재(130)와 베이스 부재(150) 사이에 형성된 적어도 하나의 채널(160a, 160b)을 포함한다. 또한 분석 유닛(100)은 베이스 리세스(152) 내에 배치된 프로브 캐리어(155)를 포함하고, 상기 프로브 캐리어는 프로브(157)로서 생화학 물질을 식별하기 위한 적어도 하나의 지표 물질을 포함하고, 프로브 캐리어(155) 상의 지표 물질은 고체 응집 상태이다.

SUCTION DEVICE FOR PIPETTES

The present invention relates to a suction device (1) to be used together with a pipette, in particular a Pasteur pipette. The suction device comprises a base element (2) with the bore (2a) for the insertion of a pipette (5), and a body connected to the base element, wherein the interior of the body is hollow, wherein at least the body is made of a flexible material, so that the inner volume of the body (3) is variable. Herein, the base element is formed in a polygonal or elliptic shape, so that the shape of the base element is not round and hence prevents a rolling of the suction device over a lab bench or the like.

MICROFLUIDIC DEVICES AND SYSTEMS, AND METHODS FOR OPERATING MICROFLUIDIC DEVICES AND SYSTEMS

A microfluidic device includes a substrate having a first fluid inlet/outlet system, a second fluid inlet/outlet system, and a fluidic network between the first fluid inlet/outlet system and the second fluid inlet/outlet system and in fluid communication with the first fluid inlet/outlet system and the second fluid inlet/outlet system. The fluidic network includes a microfluidic channel network that is in fluid communication with the first fluid inlet/outlet system and spaced from the second fluid inlet/outlet system, a nanofluidic channel network fluidly connecting the microfluidic channel network and the second fluid inlet/outlet system, and a plurality of pores in fluid communication with the microfluidic channel network and the nanofluidic channel network.

FLUIDIC DEVICE AND METHOD

A microfluidic system for fluid transport is provided. The microfluidic system includes a microfluidic device. The microfluidic device includes an inlet body including an inlet. The microfluidic device includes a base supporting the inlet body. The base includes a channel in fluid communication with the inlet. The base includes one or more sensors formed on a surface of the channel, or one or more sensors formed in one or more wells formed in the surface of the channel. The channel is configured to facilitate flow of the fluid. The fluid includes a plurality of beads. The fluid includes a plurality of suspended cells. The inlet is configured to receive the fluid at an inlet port. The inlet is configured to output the fluid through an opening in fluid communication with the channel. The inlet is configured to provide substantially uniform flow of the fluid across a substantial portion of a horizontal dimension of the channel. The device is configured to compensate for edge effects otherwise ...

Microfluidic Device For Real-Time Clinical Monitoring And Quantitative Assessment Of Whole Blood Coagulation

A microfluidic coagulation assessment device includes a plurality of microchannels, with a blood sample driven through the microchannels at a substantially constant flow rate. A controller is configured to, in combination with a timer and a pressure sensing device, determine a first pressure value (or flow value) at an initiation of flow, a first time (Tpg) at which a second pressure value is about twice the determined first pressure value, and a second time (Tpf) at which a third pressure value is about (1+e) times the determined first pressure value and establish a subject coagulation model predictive of channel occlusion therefrom.

HEAT PUMP DEVICE AND ASSEMBLY

A heat pump that includes a thermoelectric device(s) and a heat sink having a raised portion with a top surface for thermally coupling with a planar face of the thermoelectric device(s). The raised portion of the heat sink includes an outer periphery and a raised central region surrounded by a void region to provide more uniform thermal conductivity when clamped within an assembly. The raised central region is shaped in an any shape corresponding to a shape of uneven thermal conductivity due to clamping pressure applied to the heat sink. The void region can be substantially contiguous and entirely circumscribe the central raised region. The device can optionally include discrete supports formed of a less thermally-conductive material within the void region. The supports can be elastomeric, such as O-rings, and disposed within pockets defined within the void region.

Diagnostic Radio Frequency Identification Sensors And Applications Thereof

An integrated passive wireless chip diagnostic sensor system is described that can be interrogated remotely with a wireless device such as a modified cell phone incorporating multi-protocol RFID reader capabilities (such as the emerging Gen-2 standard) or Bluetooth, providing universal easy to use, low cost and immediate quantitative analyses, geolocation and sensor networking capabilities to users of the technology. The present invention can be integrated into various diagnostic platforms and is applicable for use with low power sensors such as thin films, MEMS, electrochemical, thermal, resistive, nano or microfluidic sensor technologies. Applications of the present invention include on-the-spot medical and self-diagnostics on smart skin patches, Point-of-Care (POC) analyses, food diagnostics, pathogen detection, disease-specific wireless biomarker detection, remote structural stresses detection and sensor networks for industrial or Homeland Security using low cost wireless devices such ...

Centrifugal force based microfluidic device, biochemical treatment system having the same, and method of fabricating the microfluidic device

Provided are a centrifugal force-based microfluidic device (10) in which biochemical treatments of samples are executed, a biochemical treatment system including the centrifugal force-based microfluidic device, and a method of fabricating the centrifugal force-based microfluidic device. The centrifugal force-based microfluidic device (10) includes a rotational platform (12) including a unit to perform biochemical treatments of samples and a mounting hole (13) defined within to which a turntable including a magnetic force generator is inserted; and a cap (21) coupled to the rotational platform (12) closing a side of the mounting hole (13) opposite to a side in which the turntable is inserted, and including a part formed of a ferromagnetic material pulled by magnetic force generated by the magnetic force generator.

Skin sample culture and membrane test device

METHODS FOR CONVECTIVELY-DRIVEN INTRACELLULAR DELIVERY

Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter; and a plurality of relaxation spaces disposed between the compressive surfaces; flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume (Vloss); and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume (Vgain) and absorb a portion of the plurality of molecules.

CONTROL KNOB IMPROVED PIPETTE

L'invention concerne un bouton de commande (12) d'une pipette de prélèvement à actionnement manuel, le bouton comprenant des parties inférieure et supérieure (24a, 24b) dont l'une est équipée d'un capteur d'effort (31) et l'autre d'un organe d'actionnement (30). En position verticale du bouton, dans un état non sollicité, d'une part l'organe (30) assure le maintien axial de la partie supérieure sur la partie inférieure (24a), et d'autre part ces parties définissent une liaison circulaire (42) de retenue axiale entre celles-ci, la liaison circulaire présentant un jeu axial (47) configuré de sorte que lors d'une pression par le pouce d'un opérateur sur une zone d'action excentrée (102a), le jeu axial (47) se consomme au niveau de cette zone (102a), pendant que la liaison (42) retient axialement et localement, relativement à la partie inférieure (24a), une zone de réaction (102b) agencée de façon diamétralement opposée à la zone d'action (102a).

Microfluidic device for real-time clinical monitoring and quantitative assessment of whole blood coagulation

In accord with one aspect, a microfluidic coagulation assessment device defining a plurality of microchannels is provided, wherein a blood sample is driven through the microchannels at a substantially constant flow rate and a controller is configured to, in combination with a timer and a pressure sensing device, determine a first pressure value (or flow value) at an initiation of flow, a first time (Tpg) at which a second pressure value is about twice the determined first pressure value, and a second time (Tpf) at which a third pressure value is about (1+e) times the determined first pressure value and establish a subject coagulation model predictive of channel occlusion therefrom. In another aspect, the blood sample is driven through the microchannels at a substantially constant pressure and a controller is configured to, in combination with a timer and a flow sensing device make the determination based on flow rate.

Heat pump device and assembly

A heat pump that includes a thermoelectric device(s) and a heat sink having a raised portion with a top surface for thermally coupling with a planar face of the thermoelectric device(s). The raised portion of the heat sink includes an outer periphery and a raised central region surrounded by a void region to provide more uniform thermal conductivity when clamped within an assembly. The raised central region is shaped in an any shape corresponding to a shape of uneven thermal conductivity due to clamping pressure applied to the heat sink. The void region can be substantially contiguous and entirely circumscribe the central raised region. The device can optionally include discrete supports formed of a less thermally-conductive material within the void region. The supports can be elastomeric, such as O-rings, and disposed within pockets defined within the void region.

Systems and methods for integration of microfluidic tear collection and lateral flow analysis of analytes of interest

Systems, methods, and devices for analyzing small volumes of fluidic samples, as a non-limiting example, less than twenty microliters are provided. The devices are configured to make a first sample reading, for example, measure an energy property of the fluid sample, for example, osmolality, make a second sample reading, for example, detecting the presence or concentration of one or more analytes in the fluid sample, or make both the first sample reading and the second sample reading, for example, measuring the energy property of the fluid sample as well as detecting the presence or concentration of one or more analytes in the fluid sample.

Biomolecule extraction device and biomolecule extraction method

Disclosed are a biomolecule extraction device and a biomolecule extraction method. The biomolecule extraction device and the biomolecule extraction method according to the present invention make it possible to simplify the process for extracting biomolecules such as protein and nucleic acid from a biomaterial such as tissue and cells and make it possible to reduce the time and space needed for the extraction process, thereby improving user convenience. Also, dead space may be minimized to increase the extracted biomolecule concentration, and the usage of a buffer may be reduced. Also, extracted samples may be constantly discharged to perform testing. In addition, it is unnecessary to use a separate device or tool, and damage to extracted biomolecules is minimized. Also, consistent test results may be expected even when a small amount of a sample is applied.

WHOLE BLOOD SEPARATION SAMPLING APPARATUS

The present invention provides systems, devices, kits, and methods for separating blood plasma or serum from whole blood. The present invention further provides systems, devices, and methods for separating a volume of blood plasma or serum from whole blood.

FLUID FILTERING DEVICE AND ASSEMBLY

A fluid refining device and assembly comprises an inlet for fluid to be refined, a separation outlet and a concentration outlet for processed fluid in a refining layer, wherein the refining layer comprises a plurality of refining units arranged in a pattern, and wherein the cross section of the concentration outlet is less than the cross section of the inlet.

Whole blood separation sampling apparatus

ALCOHOL LAMP NOT FALLING DOWN

The present invention relates to an alcohol lamp, and more specifically, to an alcohol lamp which is attached to a bottom surface of an alcohol lamp so as to lower the entire center of gravity downward. The alcohol lamp of the present invention is attached to an existing alcohol lamp, and does not easily fall by shock which the alcohol lamp may receive during an experiment. To this end, the present invention is configured to serve as effectively lowering the center of gravity of the entire alcohol lamp by using a substance having a high density at a lower part of a part to be attached, and preventing an accident occurring due to the alcohol lamp falling down. COPYRIGHT KIPO 2018 ...

Incubation Device Having Rotary Mechanism

An incubation device includes an actuator, a platform, and an incubation lid. The actuator includes a motion disc and a shaft connected to the motion disc. The shaft extends away from the motion disc. The platform is connected to the shaft of the actuator in a manner allowing movement transmission. The platform has a through hole and a thermal conductive plate. One end of the through hole is sealed by the thermal conductive plate. The incubation lid is movably disposed over the platform. The platform is thermal insulating. The incubation lid has an opening allowing fluid communication.

PROCESS FOR THE OBTAINMENT OF SUSPENDED DOUBLE-FLAT SU-8 JANUS CHIPS, SAID SUSPENDED DOUBLE-FLAT SU-8 JANUS CHIPS AND SUSPENDED ARRAY THEREOF

The invention relates to a process for the obtainment of suspended double-flat SU-8 Janus chips by means of SU-8 photolithography to create an arrangement of ordered SU-8 chips on a substrate and soft lithography to create a planar printed arrangement or a planar printed array on each chip, said process comprising steps for forming a solidified membrane that is peeled off and dissolved in an aqueous medium. Furthermore, the invention relates to the suspended double-flat SU-8 Janus chips and to a suspended array comprises at least two different suspended double-flat SU-8 Janus chips. Lastly, the invention relates to barcoding chips and to sensing devices comprising said suspended double-flat SU-8 Janus chips or said suspended array.

Solid reagent containment unit, in particular for a portable microfluidic device for sample preparation and molecule analysis

A solid reagent containment unit is formed by a support; a frame body fixed to the support and delimiting internally, together with the support, an analysis volume; a reagent-adhesion structure within the analysis volume; and at least one reagent cavity, which extends within the reagent-adhesion structure. The reagent-adhesion structure is of an adhesion material embossable at temperatures lower by 6-8° C. than its own melting point and has a melting point such as not to interfere with the analysis. The reagent cavity forms a retention wall, laterally surrounding the reagent cavity, and houses dried reagents. The adhesion material is chosen among wax, such as paraffin, a polymer, such as polycaprolactone, a solid fat, such as cocoa butter, and a gel, such as hydrogel or organogel.

Sample collection device

A sampling device having a lower portion with a sample container. A cap is moveably attached with the lower portion and includes a cutting edge configured for cutting a leaf. When the cap is attached to the lower portion with a leaf there between, a leaf sample is deposited into the sample container of the lower portion. The cap includes a vent in fluid communication with the sample container such that the leaf sample is dried. A detachable label can extend from the lower portion.

SELECTIVE DELIVERY OF MATERIAL TO CELLS

Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.

Patterned fluid array and preparation and use thereof

CONTROL KNOB IMPROVED PIPETTE

Portable urine detection device and method

A portable urine detection device and method is disclosed by the invention which relates to the technical field of urine detection. The invention includes a detection device and a cloud backstage system, of which the detection device comprises a shell, a slot component, a fixed component and several test strips. The circuit board is fixed in the shell. Several LED lamp beads, a camera module, a first algorithm module, a second algorithm module, a third algorithm module and a fourth algorithm module are fixed on one surface of the circuit board. One side of the circuit board is provided a USB port; one side of the USB port penetrates one side of the shell; the other side of the shell is provided with an opening; one side of the shell is connected with a smart phone through a USB port and an OTG data line. The fixed component comprises a bottom plate. According to the invention, the problems of overlarge volume, incapability of universal use and higher cost of the existing urine detection ...

Acoustophoresis device having improved dimensions

Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating target particles from elements of whole blood. The whole blood and capture particles are flowed through a microfluidic separation channel formed in a thermoplastic. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements of the blood and target particles to specific aggregation axes.

Bubble removing system

A bubble removing system and a bubble removing method are provided. The bubble removing system comprises a main bubble removing apparatus which comprises a first enclosed container, a first fluid lead-in pipe, a first fluid lead-out pipe, and a bubble collecting member. The cross section of the inner cavity of the first enclosed container is circular, and the first enclosed container is used for accommodating a fluid substance. The first fluid lead-in pipe passes through a sidewall of the first enclosed container, is tangent to the inner cavity wall of the first enclosed container, and is disposed at the upper part of the first enclosed container. The first fluid lead-out pipe passes through the sidewall of the first enclosed container and is disposed at the lower part of the first enclosed container.

SAMPLE COLLECTION DEVICE AND METHODS OF USING SAME

Described herein are various inventions and embodiments thereof, directed to systems, devices, and methods for separating a sample. Embodiments of sample collection devices disclosed herein may maintain separation of different volumes of sample during collecting, handling, and accessing of the sample. A sample collection device, in some embodiments, may comprise an enclosure defining a first chamber, a second chamber, and a partition therebetween configured to separate the first chamber and the second chamber. An assembly coupled to the partition may be configured to transition between an open configuration and a closed configuration based on fluid and gas flow through the assembly.

REAGENT EXCHANGE METHODS, DEVICES, AND SYSTEMS

Provided include a microfluidic device capable of performing reagent exchange, a gas-flow control device, a cell reaction module, and a device for preparing cell samples, such as a single-cell sample, and method of use thereof.

Continuous use syringe filter

Whole blood separation sampling apparatus

The present invention provides systems, devices, kits, and methods for separating blood plasma or serum from whole blood. The present invention further provides systems, devices, and methods for separating a volume of blood plasma or serum from whole blood.

Systems and methods for integration of microfluidic tear collection and lateral flow analysis of analytes of interest

Systems, methods, and devices for analyzing small volumes of fluidic samples, as a non-limiting example, less than twenty microliters are provided. The devices are configured to make a first sample reading, for example, measure an energy property of the fluid sample, for example, osmolality, make a second sample reading, for example, detecting the presence or concentration of one or more analytes in the fluid sample, or make both the first sample reading and the second sample reading, for example, measuring the energy property of the fluid sample as well as detecting the presence or concentration of one or more analytes in the fluid sample.

Biosensor and biosensor manufacturing method

INERTIA BASED MICROFLUIDIC INTRACELLULAR DELIVERY PLATFORMS

INERTIA BASED MICROFLUIDIC INTRACELLULAR DELIVERY PLATFORMS AND INTRACELLULAR MASS TRANSFER METHOD USING THE SAME

ASSEMBLIES AND METHODS FOR SCREENING SAMPLE FLUIDS

According to the present invention there is provided an assembly comprising, a needle unit comprising n hollow needles wherein n is greater than one, and wherein each hollow needle can receive a respective sample fluid; a flow cell unit comprising m flow cells wherein m is greater than one, each flow cell having an input and an output, and a test surface on which ligands can be provided located between the input, and output; a means for consecutively moving sample fluids, from each of said n hollow needles respectively, into all said m flow cells, so that said sample fluids flow consecutively through the same flow cells. There is further provided a corresponding method of screening a sample fluid for molecules which can bind to predefined ligands.

MICROSTRUCTURE AND MOLECULAR DETECTION METHOD

To provide a microstructure equipped with a mechanism for selectively detecting marker molecules expressed or secreted by individual cells forming a cell population, a method for fabricating such a microstructure, and specific solutions for detecting and identifying molecules to be detected using such a microstructure, the present invention provides a method for fabricating a hemispherical shell-shaped microstructure made of a thin film of a desired thickness and diameter, in which a material surface capable of fixing a probe for detecting a biomolecule is arranged on the inner surface, and a method for detecting a target biomolecule using such a thin film.

Segmented top plate for variable driving and short protection for digital microfluidics

A digital microfluidic device, comprising a bottom plate and a top plate. The bottom plate comprises a bottom electrode array comprising a plurality of digital microfluidic propulsion electrodes. The top plate comprises a segmented top electrode array comprising a plurality of separately voltage addressable top electrode segments. Each top electrode segment and at least two of the propulsion electrodes of the bottom electrode array form a zone within the device. A controller is operatively coupled to the top electrode array and to the bottom electrode array and is configured to provide propulsion voltages between the top plate segment and the bottom plate propulsion electrodes of at least one of the zones. The top plate and the bottom plate are provided in a spaced relationship defining a microfluidic region therebetween.

Improved collection and storage apparatus

This invention relates to an evidence collection and storage apparatus for use in forensic examination and sample collection processes. The apparatus consists of a container and a swab, the swab including a handle portion, shaft portion and a removable head portion, wherein the container includes a cavity adapted to store an object or substance, a lid including a portion of nanomesh and a swab head portion removing means that engages the head portion of the swab within the cavity, enabling the head portion of the swab to be removed or partially removed from the shaft portion of the swab and stored in the container.

Chemical sensor

We disclose a chemical sensing device for detecting a fluid. The sensing device comprises: at least one substrate region comprising at least one etched portion; a dielectric region formed on the at least one substrate region, the dielectric region comprising at least one dielectric membrane region adjacent to the at least one etched portion; an optical source for emitting an infra-red (IR) signal; an optical detector for detecting the IR signal emitted from the optical source; one or more further substrates formed on or under the dielectric region, said one or more further substrates defining an optical path for the IR signal to propagate from the optical source to the optical detector. At least one of the optical source and optical detector is formed in or on the dielectric membrane region.

Microfluidic device and method for manufacturing the same, and method and system for detecting the number of biomolecules

The present disclosure provides a microfluidic device and a manufacturing method therefor, and a method for detecting the number of biomolecules and a system for detecting the number of biomolecules. The microfluidic device includes a glass substrate; a plurality of first metal strips formed on the glass substrate; a photoresist covering the plurality of first metal strips; a fluid cavity formed in the photoresist; a plurality of second metal strips formed on the photoresist, wherein a head end of one first metal strip and a tail end of the other first metal strip in any adjacent two first metal strips are electrically coupled through a head end and a tail end of one second metal strip between the two first metal strips, so that the plurality of first metal strips and the plurality of second metal strips form a hollow inductor structure around the fluid cavity.

THERMAL CYCLER FOR POLYMERASE CHAIN REACTION SYSTEMS USING MICRO METAL MESH FILM

RIGID MASK FOR PROTECTING SELECTIVE PORTIONS OF A CHIP, AND USE OF THE RIGID MASK

A rigid mask protects selective portions of a chip including a plurality of wells for biochemical reactions. The rigid mask includes a supporting portion and a plurality of legs, where each leg is provided with a rigid stem and a plate. The plurality of legs are arranged and fixed with respect to the supporting portion in a way aligned to the spatial arrangement of the wells, and are configured in such a way that, when each leg is inserted into the corresponding well, the respective plate covers at least in part the bottom of the well, protecting it during a chemical/physical treatment of side walls of the wells.

Fluids concentration cup assembly with hourglass shape

A biological fluid collection cup for use with a centrifuge bucket to separate a biological fluid into its component parts is disclosed. It includes an hourglass shape with a large upper and lower portion and a narrow middle portion. A piston is to slide into and slideably be received in the lower portion and a side port is provided for withdrawal of a fluid from the narrow portion.

Whole blood separation sampling apparatus

The present invention provides systems, devices, kits, and methods for separating blood plasma or serum from whole blood. The present invention further provides systems, devices, and methods for separating a volume of blood plasma or serum from whole blood.

MICROFLUIDIC CENTRIFUGE DEVICE AND METHOD FOR PERFORMING SOLUTION EXCHANGE AND SEPARATION

A microfluidic centrifuge device for solution exchange and separation. The device includes a curved fluidic channel, a first inlet and a second inlet in fluid communication with a proximal end of the curved fluid channel, and a first outlet and a second outlet in fluid communication with a distal end of the curved fluidic channel. The curved fluidic channel has dimensions sufficient to direct a first subset of the microparticles to the first outlet by Dean drag and a second subset of the microparticles to the second outlet by inertial force.

Analyseeinheit zur Durchführung einer Polymerase-Kettenreaktion, Analysevorrichtung, Verfahren zum Betrieb einer solchen Analyseeinheit und Verfahren zum Herstellen einer solchen Analyseeinheit

Die Erfindung betrifft eine Analyseeinheit (100) zur Durchführung einer Polymerase-Kettenreaktion, wobei die Analyseeinheit (100) ein Deckelelement (130) mit zumindest einer Deckelausnehmung (135) und ein Bodenelement (150), das zumindest eine Bodenausnehmung (152) aufweist, wobei die Bodenausnehmung (152) der Deckelausnehmung (135) gegenüberliegend angeordnet ist, um eine Reaktionskammer (110) zu bilden. Ferner umfasst die Analyseeinheit (100) eine Folie (140), die zumindest im Bereich der Deckelausnehmung (135) zwischen dem Deckelelement (130) und dem Bodenelement (150) angeordnet ist. Auch umfasst die Analyseeinheit (100) zumindest einen Kanal (160a, 160b), der zwischen dem Deckelelement (130) und dem Bodenelement (150) ausgebildet ist, um ein Fluid in die Bodenausnehmung (152) der Reaktionskammer (110) einzuleiten und/oder auszuleiten. Schließlich umfast die Analyseeinheit (100) einen in der Bodenausnehmung (152) angeordneten Sondenträger (155), der als Sonde (157) zumindest ein Indikatormaterial ...

Reagent bottle with aspiration pipe

An embodiment of a reagent container includes a bottle with a pipe to reduce the effects of reagent sloshing. The bottle has an elongated base and an opposed cover connected by side walls and an end wall. A flat platform surrounded by a raised rim lies in the base opposite an opening in the cover. A ribbed pipe frictionally fits within the bottle opening and may attach to the anchor region leaving vent passages around the pipe. The pipe includes an aperture adjacent to the anchor region and oriented toward the end wall so that sloshed fluid has only a small effect on the level of reagent in the pipe during transfers. A modified blow molding process produces the anchor region by extending a pin a predetermined distance into a mold while the molded material is still plastic.

Analysis unit for a transportable microfluidic device, in particular for sample preparation and molecule analysis

An analysis unit formed by an analysis body housing an analysis chamber and having a sample inlet and a supply channel configured to fluidically connect the sample inlet to the analysis chamber. Dried assay reagents are arranged in the analysis chamber and are contained in an alveolar mass. For instance, the alveolar mass is a lyophilized mass formed by excipients and by assay-specific reagents.

Selective delivery of material to cells

Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.

SCREW-TYPE MICROFLUIDIC REACTION DEVICE

A screw-type microfluidic reaction device is disclosed. According to one embodiment of the present invention, provided is the screw-type microfluidic reaction device, which comprises: a base part formed along the longitudinal direction in the form of a screw thread on the outer circumferential surface and having a flow path groove through which a microfluid passes between the thread and the thread; and a cover part having both opened ends, formed to have a length corresponding to the length of the base part, inserted into the base part to close the outer surface of the flow path to form a screw-type flow path, and an injection part for injecting the microfluid to one side of the flow path and a discharge part for discharging the microfluid to the other side of the flow path. COPYRIGHT KIPO 2018 ...

Immunoassay test strip integrating radio frequency identification tags (RFID)

DIAGNOSTIC RADIO FREQUENCY IDENTIFICATION SENSORS AND APPLICATIONS THEREOF

Apparatus and method for calibration of non-contact thermal sensors

Biochemical assay apparatus uses a container with a sleeve of electrically-conductive material ( 300 ) to heat it. The heating is done inside a chamber and a contactless heat sensor ( 110 ) such as a thermopile or a bolometer, also inside the chamber, is used to monitor the temperature of the electrically conductive material ( 300 ). There are many factors that distort the output of the heat sensor ( 110 ), particularly as the temperature rises and properties such as emissivity change, or as time goes by and tarnishing and dust affect the heat sensor output. Because the sleeve has low thermal mass and heat transfer only has to happen over short distances, it is relatively easy to calculate a change in actual temperature of the electrically conductive material ( 300 ) when subjected to a known pulse of drive current and this property can be used to calibrate the performance of the heat sensor ( 110 ) in situ in the chamber.

Portable sample analyzer cartridge

A sample analyzer cartridge for use at a point of care of a patient such as in a doctor's office, in the home, or elsewhere in the field. By providing a removable and/or disposable cartridge with all of the needed reagents and/or fluids, the sample analyzer can be reliably used outside of the laboratory environment, with little or no specialized training.

Multilayer Hydrodynamic Sheath Flow Structure

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

Methods of forming and using a solid-phase support

Disclosed herein are methods of method of making a substrate for performing a chemical synthesis reaction.

Microfluidic system including a bubble valve for regulating fluid flow through a microchannel

A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.

Optics collection and detection system and method

Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.

Single-sided continuous optoelectrowetting (sceow) device for droplet manipulation with light patterns

A single-sided continuous optoelectrowetting (SCOEW) device for manipulating droplets retained in a fluid over the SCOEW device with dynamic patterns of low intensity light, such as from a display screen, is described. A single pair of lateral electrodes are utilized for providing a lateral electric field bias, with transport motion controlled in response to projecting light through a photoconductive layer and dielectric layer adjacent to which droplets are retained. The device is configured for optically manipulating droplets having volumes spanning over five orders of magnitude, and can be configured to perform droplet dispensing, transport, splitting, merging, mixing and other droplet manipulation functions involving any of the above on a single sided surface.

Mixing device and mixing method for mixing small amounts of liquid

The invention relates to a mixing method for mixing at least one small quantity of liquid, in which a quantity of liquid is applied, in a reaction region and at least one surface sound wave is reacted with the quantity of liquid. The invention relates further to a mixing device for mixing at least one quantity of liquid for performing the method of the present invention, a use of the device, and a method of analysis for bond strengths on surfaces.

System and method for automated generation and handling of liquid mixtures

The invention relates to a system ( 1 ) for supplying a microfluidic subsystem with liquids, comprising a first valve ( 14, 29, 46 ) and a first fluidic duct ( 10, 25, 28 ), for connecting said first valve ( 14, 29, 46 ) with said microfluidic subsystem and supplying a first liquid, and a second fluidic duct ( 11 ), for connecting with said microfluidic subsystem and supplying a second liquid characterized in that said first valve ( 14, 29, 46 ) is suitable for closing with time resolution not worse than 100 msec, and parameters of said first fluidic duct ( 10, 15, 28 ) are chosen such that the value of X 1 [Pa −1 ], defined as: X 1 [Pa −1 ]=(0.5×10 −9+1/ E 1 )(α R1 L 1 2 /A 1 ) is lower than 10 4 Pa −1 , where E 1 is the Young modulus of the material, of which said first fluidic duct ( 10, 25, 28 ) is made, L 1 is the length of the said first fluidic duct ( 10, 25, 28 ), A 1 is the surface area of the lumen of the said first fluidic duct ( 10, 25, 28 ) and α R1 is a constant characterizing the geometry of the said first fluidic duct ( 10, 25, 28 ) in an equation for the hydraulic resistance R 1 of the said first fluidic duct: R 1 =α R1 ( L 1 μ/A 1 2 ) with μ denoting the dynamic viscosity coefficient of the fluid filling the said first fluidic duct ( 10, 25, 28 ) in the measurement of R 1 . The invention relates also to a method for producing microdroplets on demand in such a system.

Body fluid analysis fixture

The present invention is intended to, in a configuration in which a liquid container for analysis sealed by a sealing member is penetrated by a penetrating member, prevent the sealing member from being unexpectedly broken to leak a liquid for analysis. Also, the present invention has: a container holder part that holds a liquid container for analysis; a penetrating member that penetrates a sealing member of the liquid container for analysis; a first moving mechanism that enables the penetrating member to be moved between a hole making position and a receding position; and a second moving mechanism that enables the penetrating member moved to the hole making position by the first moving mechanism to be moved toward the sealing member to a penetrating position where the penetrating member penetrates the sealing member.

Cryogenic vials

A vial including an end wall with a given perimeter, a side wall depending from the end wall about the given perimeter, and a plug wall depending from the end wall such that an outer surface of the plug wall is radially inward and spaced from the side wall. The plug wall has an attached end portion and a free end portion. The outer surface along the attached end portion of the plug wall is angled relative to the end wall by a first angle and the outer surface along the free end portion is angled relative to the outer surface along the attached end portion and extends at a second angle relative to the end wall with the second angle being larger than the first angle

Centrifuge and separation vessel therefore

The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation.

Single column immunological test elements

A plurality of individual single column test elements are provided for use in a clinical testing apparatus. Each test element is defined by a single test column that includes a quantity of a test material, such as gel material or a bead matrix, including a cover strip used to access the contents of the test column. Individual test elements can be stored, retained and dispensed for testing patient samples.

Apparatus for high-throughput suspension measurements

A high-throughput optical suspension characterization instrument is disclosed, which can include hydraulically separate and at least partially transparent sample containers. A selection mechanism is operative to selectively direct light from a light source ( 12 ) through different ones of the sample containers along an optical axis, and an off-axis scattering detector ( 38,24 ) is responsive to scattered light from the light source after it has interacted with a sample. Phase analysis light scattering is used to determine the electrophoretic mobility and zeta potential of samples. A second instrument is disclosed, wherein all sample containers are illuminated simultaneously. Transmitted light is collected by a camera. The electrophoretic mobility and hydrodynamic size of the samples may be determined.

Electromagnetic multiplex assay biosensor

A method is provided for determining the presence of multiple different target analytes in a liquid sample using electrophoretic separation and magnetic labels within a self-contained reaction cartridge and an external magnetic sensor for detection. Magnetic labels are bound to target analytes through specific binding elements. By electrophoretic separation, the multiple different targets can be sorted according to their specific sizes and inherent molecular charges for better detection resolution and specificity. After the separation process, the target analytes are then recognized and trapped by the detection binding elements within the reaction cartridge. A magnetic field generator provides a changeable magnetic field that causes the bounded magnetic labels and target analytes to produce a resonance disruption detectable by a magnetic sensor. The sensor can provide a digital binary value to indicate whether or not a label particle is bound and that determines the presence of target analytes.

Thermal Microvalves

The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

Systems and methods for collecting tear film and measuring tear film osmolarity

A sample receiving chip comprising a substrate that receives an aliquot volume of a sample fluid and a sample region of the substrate, sized such that the volume of the sample fluid is sufficient to operatively cover a portion of the sample region. The energy imparted into the sample fluid is transduced by the sample region to produce an output signal that indicates energy properties of the sample fluid. The sample receiving chip also includes a channel formed in the substrate, the channel configured to collect the aliquot volume of a sample fluid and transfer the aliquot volume of sample fluid to the sample region.

Valve for lab-on-a-chip systems, method for actuating and for producing valve

A substrate of a lab-on-a-chip system has two adjacent recesses, one serving as a flow channel and the other one being filled with an elastomer compound. In a first state, the elastomer compound and the substrate delimit the flow channel in a section. In a second state, the elastomer compound takes up the space in the recess in the substrate along a cross-section of the flow channel, thereby completely closing the flow channel. The substrate and the elastomer compound may be produced by injection molding techniques.

Microfluidic Cell Motility Assay

Certain isolated motile cells spontaneously migrate unidirectionally through a mechanically confined space, such as a microcapillary channel, in the absence of an external gradient (e.g., a chemical gradient). Assays and methods for detecting motile cells, and identifying chemical agents that inhibit cell migration, can include detecting the movement of motile cancer cells through a microcapillary channel.

Biosensing device

The present invention provides a biosensing device, comprising an input unit, an analysis unit, a process unit, and a set unit for storing resulting data values as the basis for calibrating the biosensing device, to set up the calibration parameters of a strip of the biosensing device.

Multi-task immunoaffinity device secured to a peripheral box and integrated to a capillary electrophoresis apparatus

The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as a modular, multi-task immunoaffinity device secured to a peripheral box and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. In one embodiment, two devices are coupled in-tandem to perform separately and sequentially microreactions and concentrations of proteins. In this embodiment, biological samples containing proteins can be subjected to proteolytic processing in the first device, and the resulting peptides subjected to selective purification and concentration in the second device.

Channel and method of forming channels

A device is made by forming sacrificial fibers on a substrate mold. The fibers and mold are covered with a first material. The substrate mold is removed, and the covered fibers are then removed to form channels in the first material.

Analysis reagent and analysis device having the analysis reagent carried therein

A reagent including a combination of a polyanionic compound and a bivalent cationic compound contains one substance selected from the group consisting of succinic acid, gluconic acid, alanine, glycine, valine, histidine, maltitol, and mannitol or at least one compound of the substance. A dry state of the reagent and deliquescence can be improved.

Integrated Microfluidic Device and Methods

A microfluidic device for analyzing a sample of interest is provided. The microfluidic device can comprise a microfluidic device body, wherein the microfluidic device body comprises a sample preparation area, a nucleic acid amplification area, a nucleic acid analysis area, and a network of fluid channels. Each of the sample preparation area, the nucleic acid amplification area and the nucleic acid analysis area are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysis of a biological sample.

Microfluidic device and hemoglobin measurement method using the same

A microfluidic device and a method for measurement of biomaterials using the same. The microfluidic device includes a microfluidic structure including: a sample chamber which receives and accommodates blood; a reagent chamber which contains a luminescent reactant; a first detection chamber which contains a first material that is positively charged; a second detection chamber which is connected to the first detection chamber, and contains a second material having a boronate moiety; and at least one channel which connects the sample chamber, the reagent chamber and the first and second detection chambers.

Valve with material having modifiable degree of penetrability

The invention relates to a valve ( 2 ) for controlling a passage of particles from a first region ( 6 ) to a second region ( 7 ), wherein the valve ( 2 ) comprises a valve material ( 4 ) having a modifiable degree of penetrability and a valve region ( 16 ) comprising the valve material ( 4 ), wherein the valve region ( 16 ) and the valve material ( 4 ) are adapted such that the particles have to penetrate the valve material ( 4 ) if the particles pass the valve ( 2 ) for being transferred from the first region ( 6 ) to the second region ( 7 ). The degree of opening of the valve ( 2 ) can easily be controlled by modifying the degree of penetrability of the valve material ( 4 ), for example, by modifying the temperature of the valve material ( 4 ). Moreover, by penetrating the valve material ( 4 ) the particles can be separated from other elements like a fluid containing the particles.

Polymer microfluidic biochip fabrication

Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.

Multi-Shell Microspheres With Integrated Chromatographic And Detection Layers For Use In Array Sensors

The development of miniaturized chromatographic systems localized within individual polymer microspheres and their incorporation into a bead-based cross-reactive sensor array platform is described herein. The integrated chromatographic and detection concept is based on the creation of distinct functional layers within the microspheres. In this first example of the new methodology, complexing ligands have been selectively immobilized to create “separation” layers harboring an affinity for various analytes. Information concerning the identities and concentrations of analytes may be drawn from the temporal properties of the beads' optical responses, Varying the nature of the ligand in the separation shell yields a collection of cross-reactive sensing elements well suited for use in array-based micro-total-analysis systems.

Apparatus and method for the detection and analysis of particles in fluids

The invention relates to apparatus for the detection of particles and for particle analysis. The apparatus comprises a sample holder comprising a base and a projection extending from the base. The base includes a contact region where, in use, the surface of a fluid sample may contact the projection. The surface of at least the contact region of the projection exhibits properties that allow the surface of the contact region to be substantially wetted by a fluid sample when the apparatus is in use so that the fluid sample forms a meniscus having its apex in contact with the contact region of the projection. The invention also relates to methods for using the apparatus.

Variable Volume Mixing and Automatic Fluid Management for Programmable Microfluids

A microfluidic arrangement including a fluid channel configured to receive a first fluid from a first inlet and a second fluid from a second inlet, and a mixer connected to the fluid channel, the mixer including a mixer channel configured to receive a volume of the first fluid and a volume of the second fluid from the fluid channel, the mixer channel defining a mixer capacity, wherein the mixer is (i) configured to mix the volume of the first fluid and the volume of the second fluid in order to provide a mixture of the first fluid and the second fluid when the combined volume of the first fluid and the second fluid is less than the mixer capacity, and (ii) further configured to mix the volume of the first fluid and the volume of the second fluid in order to provide a mixture of the first fluid and the second fluid when the combined volume of the first fluid and the second fluid is equal to the mixer capacity.

Specimen Collection Container Assembly

A specimen collection container includes inner and outer tubes. The inner tube includes a bottom end, a top end, and a sidewall extending therebetween defining an interior. The sidewall includes an inner surface and an outer surface having at least one annular protrusion extending therefrom. The inner tube includes at least one funnel portion adjacent the top end for directing a specimen into the inner tube interior, and an annular ring disposed about a portion of the outer surface of the sidewall adjacent the top end. The outer tube includes a bottom end, a top end, and a sidewall extending therebetween, the sidewall having an outer surface and an inner surface defining an annular recess adapted to receive a portion of the annular protrusion therein. The inner tube is disposed within the outer tube and a portion of the top end of the outer tube abuts the annular ring.

System and method for microfluidic flow control

A system and method for controlling fluid flow within a microchannel includes a fluid circuit comprising a fluid outlet well and one or more fluid inlet wells, all in communication with a microchannel. A negative pressure differential is applied to the outlet well and fluid flow from an inlet well into the microchannel is controlled by opening or closing the inlet well to atmospheric pressure. To stop fluid flow from the inlet well, a negative pressure differential may be applied to the inlet well to equalize pressure between the inlet and outlet wells. By sequentially opening and closing different inlet wells to atmosphere, controlled amounts of different reagents can be serially introduced into the microchannel.

Liquid feeding system for microchip, sample detection device, and liquid feeding method for liquid feeding system for microchip

A liquid feeding system for a microchip performs: a first liquid feeding step in which a sample liquid in a sample liquid containing section is fed in the direction to a primary containing section via a reaction field; a second liquid feeding step in which, after the first liquid feeding step, the sample liquid is fed from the primary containing section in the direction to the reaction field; and a third liquid feeding step in which, after the second liquid feeding step, the feedings of the sample liquid from and to the reaction field and the primary containing section a rear side gas-liquid boundary face of the sample liquid in the first liquid feeding step and the front side and rear side gas-liquid boundary faces of the sample liquid in the second and third liquid feeding steps do not pass through the reaction field.

Micro-fluidic test apparatus and method

An apparatus, system, and method for determining the osmolarity of a fluid. The apparatus includes at least one micro-fluidic circuit and at least one electrical circuit disposed in communication with the micro-fluidic circuit for determining a property of a fluid contained within the at least one micro-fluidic circuit.

Biological microfluidics chip and related methods

A biological microfluidics chip ( 100 ) comprising a substrate ( 102 ), a microfluidic inlet port ( 104 ) defining an opening in a surface of the substrate ( 102 ), and a microfluidic outlet port ( 106 ) defining an opening in a surface of the substrate ( 102 ). The biological microfluidics chip ( 100 ) also comprises a plurality of wells ( 108 ) extending from a top surface ( 110 ) of the substrate, wherein each well ( 108 ) is bounded by one or more walls, and an inlet opening ( 112 ) and an outlet opening ( 114 ) are provided in a wall of each of the plurality of wells ( 108 ). An inlet microfluidic channel ( 116 ) is provided in the substrate ( 102 ) to connect the microfluidic inlet port ( 104 ) to each of the inlet openings ( 112 ) in the walls of the wells, and an outlet microfluidic channel ( 118 ) is provided in the substrate to connect each of the outlet openings ( 114 ) in the walls of the wells to the microfluidic outlet port ( 106 ).

Rapid Test Apparatus

Provided herein are methods and devices for rapid testing of solid, semi-solid, or liquid specimens, such a stool, blood, urine, saliva, or swab specimens of the cervix, urethra, nostril, throat, and for environmental testing.

Quantitative and self-calibrating chemical analysis using paper-based microfluidic systems

A method of determining the concentration of a test fluid sample using a paper-based microfluidic system having a plurality of hydrophilic testing zones, including: a) depositing said test fluid sample on at least one said testing zone; b) depositing a plurality of standard fluid samples or reactives of differing known concentrations on other said testing zones; c) introducing an indicator solution to each said test zone to thereby react with the deposited fluid sample and result in a colour intensity change which is a function of the fluid sample concentration; and d) comparing the differences in colour intensity between the test fluid sample and the standard fluid samples or reactives to thereby determine the concentration of said test fluid sample.

Luer seal for solid phase extraction columns

Disclosed herein is a luer seal for sealing a bottom outlet of each of the wells or columns in a plurality of Solid Phase Extraction (SPE) wells or columns in an array, the luer seal comprising a flexible, substantially flat mat having a top surface and a bottom surface, the top surface having portions defining a plurality of female luer slip connectors spaced for receiving an open tip of at least one of the plurality of solid phase extraction wells or columns, and sealingly fastening thereto for preventing fluid flow through the open tip. Also disclosed is a luer seal extractor for removing the luer seal from the column tips substantially simultaneously.

Microfluidic reactor system

A compact device for operatively coupling a solid planar substrate, for example a glass slide, to a microfluidic circuit and performing a reaction or reactions on organic matter bound to the face of the planar substrate. Typical reactions include binding, staining and/or labeling reactions. In use, a sealed reaction chamber is formed, the chamber enclosing the organic matter and at least a part of the solid substrate. Headspace in the sealed chamber between the solid substrate is generally of microfluidic dimensions, and diaphragm pump members are used to inject, exchange and/or mix the fluids in the chamber.

Multiple laminar flow-based particle and cellular separation with laser steering

The invention provides a method, apparatus and system for separating cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One exemplary method includes providing a first flow having a plurality of components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first cellular component of the plurality of components into the second flow while concurrently maintaining a second cellular component of the plurality of components in the first flow. The second flow having the first cellular component is then differentially removed from the first flow having the second cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

Selective bond reduction in microfluidic devices

The invention overcomes the limitations described for the bonding of structured layers by providing a method for selectively reducing the bonding of materials. In its most generic form, the invention uses a bonding technique in combination with a printing method for modifying or covering at least one portion of a surface to either fully or partially prevent localised bonding. The structuring process may act upon the layers either before or after the bonding of the layers. The invention overcomes the limitations described in the application of affinity chromatography by providing a planar substrate with discrete optical detection flow cells that contain porous material and have connecting microchannels for fluid delivery and/or removal, and a method for making the same.

Cell concentration, capture and lysis devices and methods of use thereof

The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Test device, reaction apparatus and reactive test method

A test device having a micro flow channel including a reaction part where a reactant that is reactive to a tested chemical dispersed in a tested fluid is fixed, and at least one actuator for actuating the tested fluid to move in at least one of two opposite sides of the micro flow channel so as to homogenize a density distribution of the tested chemical in the tested fluid. The tested fluid is sent in the micro flow channel a plurality of times.

Apparatus and methods for microfluidic applications

Non-rigid tape apparatus and fabrication methods for microfluidic processing applications such as gel electrophoresis are provided, where microfluidic processing is performed on selected areas. Parts of the tape are formed by high pressure plastic film forming. Membranes and other structures are self sealing during and after penetration by pipettes and electrical probes. Rigid exoskeleton elements protect the non-rigid parts during processing and facilitate transport of the tape.

Devices and method for enrichment and alteration of cells and other particles

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

Three-dimensional (3d) hydrodynamic focusing using a microfluidic device

A microfluidic device comprises inlets for a sample flow and an out-of-plane focusing sheath flow, and a curved channel section configured to receive the sample flow and out-of-plane focusing sheath and to provide hydrodynamic focusing of the sample flow in an out-of-plane direction, the out-of-plane direction being normal to a plane including the curved channel. Examples of the invention also include improved flow cytometers.

Micro mixer and microfluidic chip

There are provided a micro mixer capable of efficiently mixing at least two types of liquids in a simple structure and a microfluidic chip provided with the micro mixer. In order to achieve the object, the micro mixer includes a minute passage through which first and second liquids are caused to flow respectively, and a mixing vessel in which a liquid injecting port caused to communicate with the minute passage is provided in a bottom part, and the liquid injecting port is provided in a shifted position from a center of the bottom part in the bottom part. Moreover, the liquid injecting port may be provided in a shifted position from a center line of the mixing vessel.

Minimally invasive cytometry system with qcl inspection of single cells for cancer detection

This disclosure concerns a minimally invasive cytometry system including a handling system that presents single cells to at least one QCL laser source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce vibrational bond absorption in one or more analytes within the cell. The cytometry system also includes a detection facility that detects the mid-infrared wavelength light transmitted by the cell and identifies the cell as either cancerous or non-cancerous.

Control of operation conditions within fluidic systems

The invention provides methods of controlling environmental conditions within a fluidic system, where such environmental conditions can affect the operation of the system in its desired function, and fluidic channels, devices, and systems that are used in practicing these methods. Such methods are generally directed to environmental control fluids, the movement of such fluids through these systems, and the interaction of these fluids with other components of the system, e.g., other fluids or solid components of the system.

Sample Processing Device

A floating chamber set may include a plurality of containers coupled to a frame such that each container translates vertically within a limited vertical range independent of the other containers. Each container may be perforated to allow liquid penetration.

Device and method for particle complex handling

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

Nanopore Device for Reversible Ion and Molecule Sensing or Migration

Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

Method for conducting multiple reactions in a single reaction tube

There is disclosed a method for conducting at least two reactions in a reaction tube, said method comprising the steps of providing at least two reaction phases within said reaction tube for allowing said reactions to occur therein, providing a separation phase that is immiscible with said two reaction phases and which is disposed therebetween, providing at least one particle capable of being coupled to a chemical species, wherein said particle is movable between said reaction phases to introduce said chemical species thereto.

Devices and methods for interfacing microfluidic devices with macrofluidic devices

The present disclosure is directed generally to devices and methods with the purpose of interfacing microfluidic devices with macrofluidic devices. Specifically, the present disclosure includes the de-

Temperature control system

For heating or cooling a sample contained in a vessel portion through a heat transfer member held in contact with the vessel portion, there is used the vessel portion, which has a part formed of an elastic member, expands and contracts for injection and discharge of the sample, is closed other than a connecting port with a channel connected to the vessel, and expands and contracts for injection and discharge of the sample. The vessel portion expands correspondingly to the injection when the sample is injected through an inflow path serving as the channel into the vessel portion contracting in a non-contacting state with the heat transfer member. A predetermined amount of sample is injected into the vessel portion so as to expand the vessel portion, and the vessel portion comes into contact with the heat transfer member. The vessel portion is heated or cooled through the heat transfer member.

Carrier with flexible microassay device and methods of use

The disclosure provides a carrier for flexible microassay devices ( 510 ). The carrier is detachably attached to the microassay device ( 510 ) and provides means for protecting and handling the flexible microassay device ( 510 ). A method of preparing a microassay device for use is also disclosed.

Breath ketone detector

Ketoacidosis is an extreme and uncontrolled form of ketosis, which is a normal response to prolonged fasting. Embodiments of this invention test the ketone level of a patient by measuring the ketone bodies in breath condensation. Some embodiments include a device for medical testing comprising a hollow container, comprising powder mixture of sodium nitroferricyanide, ammonium sulfate and silica and a liquid including an ammonium hydroxide solution.

Device for a test strip holder, method and arrangement

For the simplified use of a test strip holder, a device having a holding or positioning device is provided, comprising a mixing chamber, a lid for closing the mixing chamber, and an obvious fluid opening for a fluid passage from the mixing chamber to the test strip holder. This allows for the test strip holder to be inserted into the device in a simple way and a sample can be prepared in the mixing chamber of the device. The device is provided with a mixing chamber that can be closed, wherein a reaction partner, for example a gold conjugate, can be dissolved in the sample for increasing the sensitivity of the test strip.

Systems and methods for high-throughput detection of an analyte in a sample

Provided are high-throughput detection systems. The systems include a magnetic sensor device, a magnetic field source and a reservoir plate that includes a plurality of fluid reservoirs. The magnetic sensor device includes a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end. Also provided are methods in which the subject high-throughput detection systems find use.

Apparatus for detecting target molecules and related methods

An apparatus for analysis of a sample and in particular of a biological sample. The apparatus contains a microfluidic chip with dies, adapted to be selectively activated or deactivated by presence of target molecules in the biological sample. The apparatus further contains a light source to emit light for illumination of the microfluidic chip and an optical filter to allow passage of the light from the dies once activated or deactivated by the presence of the target molecules. A method for pressurizing a microfluidic chip is also disclosed, where a chamber is provided, the chamber is connected with the microfluidic chip and pressure is applied to the chamber.

Structures for controlling light interaction with microfluidic devices

Systems and methods for improved measurement of absorbance/transmission through fluidic systems are described. Specifically, in one set of embodiments, optical elements are fabricated on one side of a transparent fluidic device opposite a series of fluidic channels. The optical elements may guide incident light passing through the device such that most of the light is dispersed away from specific areas of the device, such as intervening portions between the fluidic channels. By decreasing the amount of light incident upon these intervening portions, the amount of noise in the detection signal can be decreased when using certain optical detection systems.

Decorative elements provided with a curled or crimped configuration at point of sale or point of use

Decorative grass and methods of providing same are disclosed wherein the decorative grass is either maintained in flattened configuration until restraint is removed and/or the decorative grass is curled and/or crimp at a point of use and/or sale.

Thermal cycling apparatus and method

A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.

Test strip construction

A test strip construction includes a test strip having a testing region bearing a chromogenic reagent to detect the presence of a substance in a fluid test sample. A supporting member is attached to one end of the test strip in a manner such that the testing region is remote form the attachment point and is free from any contact with supporting member. When dipped into the test sample the test strip can flutter away from the supporting member to allow for full contact between that the testing region and the test sample. When removed from the test sample the supporting member provides a consistent background for observation of color changes of the reagent.

Pipetting device

The invention relates to a sealing assembly for a metering device, in particular a multi-channel metering device. The sealing assembly serves to seal pipette tips with respect to the metering device. A multi-channel metering device comprises a multiplicity of openings arranged in one plane. A sealing plate with holes in the grid of the openings abuts this plane. Each pipette tip is provided with an annular collar which has an abutting face and is pressed against the sealing plate by force closure so as to enclose one hole.

Fluid handling apparatus and fluid handling system

Chip body 130 in which a through hole or concave is formed, intermediate film 140 , on one surface of which adhesive layer 150 ′ is formed and lower film 170 , on one surface of which transfer function layer 160 is formed, are prepared. Intermediate film 140 and lower film 170 are bonded together such that transfer function layer 160 is embedded in adhesive layer 150 ′ to form a laminated body. The laminated body and chip body 130 are bonded together by thermocompression to manufacture micro-chip 100.

Device and apparatus

A device ( 1 ) for carrying out a chemical or biochemical reaction and detecting the results, such as an assay to detect a target nucleic acid in a sample, said device comprising (i) a first well ( 8 ) in which a chemical or biochemical reaction such as a nucleic acid amplification reaction may be effected in a liquid phase or a receiving means for such a first well; (ii) a first channel ( 16 ) extending from the first well; (iii) a lateral flow assay device ( 32 ) arranged to receive liquid contents from said first channel, optionally by way of second well, on a bibulous membrane thereon, wherein said membrane contains elements that are able to detect the products of the chemical or biochemical reaction such as a target nucleic acid. Methods for using such devices and apparatus for carrying out assays using these are also described and claimed.

Microfluidic system and method for automated processing of particles from biological fluid

A microfluidic system for automatically depleting particles not of interest from a biological sample, comprising: a sampling module configured to receive the sample; and one or more microfluidic protein and nucleic acid depletion modules fluidically coupled to the sampling module and comprising binding agents configured to selectively bind to abundant plasma proteins or nucleic acids. A method for automatically depleting particles not of interest from a sample, comprising: receiving the sample; subjecting the sample to a force that separates at least a portion of the particles not of interest from the sample, thereby isolating at least a portion of the target component; passing the isolated target copmonent into a chamber; circulating the isolated target component in the chamber; and selectively capturing proteins or nucleic acids with binding agents within the chamber.

Sanitary swab collection system, microfluidic assay device, and methods for diagnostic assays

Biohazard specimen collection containers are provided with an external disposable skin, that is stripped away and discarded after the biohazardous specimen is collected, thus reducing or eliminating objectionable or dangerous residues on the outside surfaces of the container. Further, we teach that the sample collection container with external disposable skin may also serve as an integrated microfluidic biosample processing and analytical device, thereby providing a single entry, disposable assay unit, kit and system for “world-to-result” clinical diagnostic testing. These integrated assay devices are provided with synergic, multiple safe-handling features for protecting healthcare workers who handle them. The modified collection containers and analytical devices find application, for example, in PCR detection of infectious organisms or pathogenic markers collected on a swab.

Unit and Device for the Preparation of Cells and/or Particles in a Liquid and Method for Microscopic Analysis

A unit ( 10 ) for the preparation of cells contained in a liquid comprises a storage chamber ( 20 ) configured to store the liquid containing the cells and to release the stored liquid via an exit opening ( 22 ) upon the application of a predetermined external force, in particular a centrifugal force. A passage ( 30 ) adjacently arranged to the exit opening ( 22 ) has a cross-section larger than that of the exit opening ( 22 ). The wall at the transition from the exit opening ( 22 ) to the passage ( 30 ) forms an edge ( 32 ). The unit further comprises an observation member ( 50 ) for receiving the released liquid and an absorbing means ( 40 ) arranged adjacent to the observation member ( 50 ) between the passage ( 30 ) and the observation member ( 50 ). The absorbing means ( 40 ) has an aperture ( 42 ) allowing the released liquid to travel onto the observation member ( 50 ), and removes the liquid so as to leave the cells on the observation member ( 50 ) for observation.

Automated analyzer for clinical laboratory

A laboratory automation system that is capable of carrying out clinical chemistry assays, immunoassays, amplification of nucleic acid assays, and any combination of the foregoing, said laboratory automation system employing at least one of micro-well plates and deep multi-well plates as reaction vessels. The use of micro-well plates as reaction vessels enables the laboratory automation system to assume a variety of arrangements, i.e., the laboratory automation system can comprise a variety of functional modules that can be arranged in various ways. In order to effectively carry out immunoassays by means of micro-well plates, a technique known as inverse magnetic particle processing can be used to transfer the product(s) of immunoassays from one micro-well of a micro-well plate to another.

Sample processing cartridge and method of processing and/or analysing a sample under centrifugal force

The present invention relates to a sample processing cartridge ( 10 ) for carrying out processing under centrifugal force acting in at least two directions as the orientation of cartridge relative to centrifugal force is changed, the cartridge comprising: a first cavity ( 18 ) adapted to contain a sample; and a second cavity ( 22 ) in fluid communication with the first cavity, wherein the first and second cavities are arranged such that the sample in the first cavity is moved therefrom to the second cavity as a centrifugal force acting on the cartridge is changed from a first direction ( 30 ) to a second direction ( 36 ), the first cavity is elongated perpendicular to the centrifugal force acting in the first direction, and the second cavity is more shallow than the first cavity and more extended in the direction of the centrifugal force acting in the second direction than the first cavity is extended in the direction of the centrifugal force acting in the first direction. The present invention also relates to a method of processing and/or analysing a sample under centrifugal force.

Novel Electrostatically Addressable Microvalves

A method of controlling a main fluid in a conduit using a microvalve is described. The microvalve includes a corresponding actuation aperture in an actuation aperture layer. A control fluid flows through the actuation aperture in response to an electric field applied via a charge distribution near an actuation aperture layer. In one embodiment, the electric field may adjust the opening and closing of the actuation aperture thereby controlling the flow of the control fluid. In a second embodiment, the control fluid is an electrorheological fluid where the electric field controls the viscosity of the ER fluid, thereby controlling fluid flow through the actuation aperture. In both embodiments the flow of the control fluid controls stretching of a flexible membrane into and out of the conduit, thereby controlling the flow of the main fluid by opening or closing the conduit.

Integrated modular unit including an analyte concentrator-microreactor device connected to a cartridge-cassette

The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as an integrated modular unit and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. The integrated modular unit includes an analyte-concentrator-microreaction device connected to a modified cartridge-cassette.

Microfluidic device and method of manufacturing the microfluidic device

A microfluidic device having a substrate with an array of curvilinear cavities. The substrate of the microfluidic device is preferably fabricated of a polymer such as polydimethylsiloxane. The microfluidic device is manufactured using a gas expansion molding technique.

Cartridge for conducting a chemical reaction

A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.

Capillarity-based devices for performing chemical processes and associated systems and methods

The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a base configured to receive one or more fluids, a porous wick carried by the base portion, and a flow-metering element along the porous wick to modify a rate or volume of fluid flow along the porous wick. The porous wick can comprise a first pathway, a second pathway, and an intersection at which the first pathway and the second pathway converge. Input ends of the first and second pathways can be wettably distinct. Upon wetting of the input ends, fluid is configured to travel by capillary action along each pathway. The device may also include volume-metering features configured to automatically and independently control or modify a volume of fluid flow along one or more pathways of the porous wick.

Systems and methods for valving on a sample processing device

A system and method for valving on a sample processing device. The system can include a valve chamber, a process chamber, and a valve septum located between the valve chamber and the process chamber. The system can further include a fluid pathway in fluid communication with an inlet of the valve chamber, wherein the fluid pathway is configured to inhibit a liquid from entering the valve chamber and collecting adjacent the valve septum when the valve septum is in a closed configuration. The method can include rotating the sample processing device to exert a first force on the liquid that is insufficient to move the liquid into the valve chamber; forming an opening in the valve septum; and rotating the sample processing device to exert a second force on the liquid to move the liquid into the valve chamber.

Instrument And Method For The Automated Thermal Treatment Of Liquid Samples

An instrument and a method for the automated thermal treatment of liquid samples are disclosed. An inter-distance between a temperature-controlled receptacle for loading with a plurality of vessels for containing the samples and end portions of optical fibers can be varied, wherein the receptacle is configured to form a thermal communication with the loaded vessels and wherein the optical fibers have first and second end portions. The first end portion and the second end portion of each optical fiber is fixed with respect to each other for transmitting light, wherein the variation of the inter-distance allows the vessels to be loaded to or unloaded from the receptacle and to enable detection of light from the samples contained in the one or more receptacle-loaded vessels.

Novel enhanced surface area conico-cylindrical flask (es-ccf) for biofilm cultivation

A novel enhanced surface area conico-cylindrical flask (ES-CCF) providing increased surface area by virtue of its' inner arrangement and useful in routine small-scale studies of bioactives production by any biofilm-forming marine as well as terrestrial microorganisms. Compared to corresponding Erlenmeyer flask of similar volume the ES-CCF provides more than 80% additional surface for biofilm attachment and growth. The ES-CCF does not require steam sterilization and is durable as the device is constructed of polymethyl methacrylate or any other such hydrophobic material and offers possibility of altering the nature of the growth surface (hydrophilic and hydrophobic). The ES-CCF also provides external aeration like a bioreactor, thus increasing the versatility of applications. Further, the device can be operated as a cylindrical flask, that is without the inner arrangement.

Microchemical nanofactories

Embodiments of an apparatus, system, and method for chemical synthesis and/or analysis are disclosed. One embodiment of a disclosed apparatus comprises a laminated, microfluidic structure defining a reactor and a separator. Such apparatuses, or portions thereof, generally have dimensions ranging from about 1 micrometer to about 100 micrometers. To implement synthetic processes, disclosed embodiments of the apparatus generally include at least one unit operation, such as a mixer, a valve, a separator, a detector, and combinations thereof. Individual apparatuses may be coupled both in series and in parallel to form a system for making chemical compounds. An individual apparatus or a system also can be used in combination with known devices and processes.

Sheath flow devices and methods

The invention relates generally to fluid processing and, in particular aspects, processing fluids for detection, selection, trapping and/or sorting of particulate moieties. Sheath flow devices described allow isolation of target species from fluid samples while avoiding non-specific binding of unwanted species to the surfaces of the separation device. Biological fluid processing, detection, sorting or selection of cells, proteins, and nucleic acids is described. The invention finds particular use in diagnostic settings, analyzing a patient's medical condition, monitoring and/or adjusting a therapeutic regimen and producing cell based products.

Particle Analysis in an Acoustic Cytometer

The present invention is a method and apparatus for acoustically manipulating one or more particles.

Methods and apparatuses for convective polymerase chain reaction (pcr)

The present invention provides a method and apparatus for amplifying a nucleic acid sequence by polymerase chain reaction (PCR). The method comprises placing a PCR sample in a container which is heated by only a single heat source that provides a high temperature for denaturation in the bottom of the PCR sample, while annealing and extension automatically occur in different regions of the PCR sample due to the convection induced by a temperature gradient descending from the bottom of the PCR sample to the surface of the PCR sample.

Microchip for nucleic acid amplification reaction and a method of manufacturing the same

To provide a microchip for a nucleic acid amplification reaction which allows high-precision analysis by a simple method. There is provided a microchip A for a nucleic acid amplification reaction including an entrance through which a liquid enters from the outside, a plurality of wells configured to function as reaction sites of nucleic acid amplification reaction, and flow channels through which the liquid entered from the entrance is fed into each of the wells, in which a plurality of reagents needed for the reaction are laminated and anchored in a prescribed order in each well.

Biosample plate with data storage and wireless communication means

Embodiments of the disclosure relate to a biosample plate that includes a memory component for storing information related to the biosample, biosample plate and biosample analysis data, and a wireless communication interface for transferring information to and from the biosample plate. The biosample plate may be used with an analyzing and data recording system such as an electromagnetic tape drive. The disclosed biosample plate facilitates the correlation between a large number of biosample plates and data as data remains with the corresponding biosamples both when the biosample plates are in use and when they are in storage. The wireless communication interface may comprise an antenna disposed in a biosample plate for data transmission to and from the biosample plate by radio signals.

Multi-Sample Particle Analyzer and Method for High Throughput Screening

Embodiments of the present invention provide a system and method for analyzing a plurality of samples comprising obtaining with an autosampler a plurality of samples from a first plate having a plurality of sample wells wherein the autosampler has a plurality of probes for sampling a set of samples and wherein each probe of the plurality of probes is in communication with a separate flow cytometer via a separate conduit. The plurality of samples comprising particles is moved into a fluid flow stream for each separate conduit. Adjacent ones of the plurality of samples are separated from each other in the fluid flow stream by a separation gas, thereby forming a gas-separated fluid flow stream. The gas-separated fluid flow stream is independently guided to and through each separate flow cytometer.

Device, fluidic module and method for producing a dilution series

A device for producing a dilution series from a solution to be diluted, which contains a substance to be diluted, and a dilution solution, includes a body of rotation, a drive configured to subject the body of rotation to rotations having different rotation protocols, and a controller configured to control the drive so as to subject the body of rotation to the different rotational frequencies. A first mixing chamber and a second mixing chamber are connected via a fluidic connection which enables producing, in the first mixing chamber, a first mixture having a first dilution ratio, transferring a partial volume of the first mixture into the second mixing chamber, and there producing a second mixture having a second dilution ratio.

Analytical test element and process for its production

An analytical test element is provided having a surface comprising a chemical detection layer on which a spreading net is disposed, the spreading net being configured to provide for the planar distribution of a liquid sample on the detection layer. The spreading net generally comprises a filament structure coated with a metallic layer that is oxidized at least on one or more surface portions thereof facing the detection layer.

Assembly and method for the filtration of a liquid and use in microscopy

An assembly and method are disclosed for the filtration of a liquid and the use thereof, wherein a supporting body is designed in a recess of a carrier and a filter membrane lies flat on the supporting body. The filter membrane and the supporting body are designed to be permeable to liquids and thus serve as filters, in particular for filtering tumor cells from blood. The carrier can having standard shapes of an object carrier for microscopy and the filtration residue on the filter membrane can be easily handled and examined in the microscope. As a result of the filter membrane lying level on the supporting body, the filtration residue can be particularly well examined microscopically.

Microfabricated elastomeric valve and pump systems

A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Method of Pre-Treatment and Staining of a Biological Sample and Device for Support of a Biological Sample and Methods of Using Such Device

There is disclosed a method of pre-treatment and staining, according to a protocol, of a biological sample disposed upon the surface of a carrier, the method comprising the step of recording at least one parameter relating to at least one protocol step in a non-volatile memory located either upon or within the carrier or a device incorporating the carrier. Also disclosed is a device comprising: a non-volatile memory; a surface of the device adapted to carry a biological sample; and communications means electrically coupled to the memory for enabling data transmission to or from an external apparatus. Also disclosed is a method of controlling processing of a biological sample disposed upon a carrier, comprising: providing, upon or within the carrier or an apparatus holding the carrier, a non-volatile memory having information relating to sample processing priority or protocol; reading the information; and scheduling the processing based upon the information.

System for Treating Liquids

A system for treating liquids comprising: a fluidics unit having at least one functional unit; at least one liquid storage unit of the first type; at least one liquid storage unit of second type; a first liquid line; and a second liquid line, wherein a flow of liquid through the first liquid line directed from the fluidics unit to the liquid storage unit of first type is blocked, at least at times, by means of a first valve and a flow of liquid through the second liquid line in the direction from the liquid storage unit of second type to the fluidics unit is blocked, at least at times, by means of a second valve.

Method and system for transferring and/or concentrating a sample

A method of controlling the concentration of a sample flowing through a microchannel is provided. The method includes flowing a sample carrier fluid from a sample carrier channel and into an upstream portion of a microchannel such that the sample carrier fluid flows from the upstream portion and toward a downstream portion of the microchannel, and flowing a sheathing fluid from a sheathing fluid channel and into the upstream portion of the microchannel such that the sheathing fluid flows from the upstream portion and toward the downstream portion of the microchannel, and generally parallel to the sample carrier fluid. The method further includes detecting a concentration of a sample in the sample carrier fluid in the downstream portion of the microchannel, and adjusting a flow rate of at least one of the sample carrier fluid and the sheathing fluid based upon the detected concentration of the sample.

Filter device

A filter device of the present disclosure includes a first port from which a solution containing a substance is to be input, and a first flow passage communicating with the first port. A filter portion made of a plurality of fibrous substances including inorganic oxide is formed in at least one part in the first flow passage. The plurality of fibrous substances has one peak in the diameter distribution.

Analytical Cartridge with Fluid Flow Control

Analytical cartridges, systems and methods of processing a sample for analysis using capillary flows. Vertical gradient sample filtration provides filtrate to an incubation chamber for a time controlled by a flow modulator at the outlet of the incubation chamber. The flow modulator can include a serpentine capillary flow path without side walls. Incubated filtrate can flow from the incubation chamber to a detection channel after a predetermined time. The detection chamber can include one or more analytical regions in a porous substrate for detection of two or more analytes on the same cartridge from the same sample.

Dna analyzer

Aspects of the disclosure provide a microfluidic chip to facilitate DNA analysis. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, a dilution domain coupled to the first domain to dilute a PCR mixture received from the first domain, and a second domain that is coupled to the dilution domain so as to receive the amplified DNA fragments. The second domain includes a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. In addition, the disclosure provides a DNA analyzer to act on the microfluidic chip to perform an integrated single chip DNA analysis.

Immunoassays, methods for carrying out immunoassays, immunoassay kits and method for manufacturing immunoassay kits

The invention relates to immunoassays, methods for carrying out immunoassays, immunoassay kits and methods for manufacturing immunoassay kits. In particular, the invention has relevance to capillary (especially microcapillary) immunoassay technology.

Fluid sample collecting and analyzing apparatus and method

A spongy swab ( 16 ) is mounted against the distal face ( 15 ) of a piston ( 14 ) at the end of a push-rod ( 12 ). Once humected with a sample fluid, the swab and piston assembly is inserted into a tubular body ( 30 ) like the plunger of a syringe. As the swab is pushed and squeezed against the distal end ( 20 ) of the body up to a trippable stop, part of the fluid is excreted into a chromatographic immunoassay testing device ( 21 ) through a first aperture ( 19 ) in a distal section of the body. The remainder of the sample is kept in a sealed chamber ( 7 ) between the piston and the end wall of the tubular body until it is excreted through a second aperture ( 23 ) for a secondary test by pushing the piston beyond the trippable stop. That preserves the sample within the spongy swab that has been used to collect it; thus preventing the adsorption of the analytes on the surfaces of the sealed chamber, and to provide a convenient and rapid way to extract the remainder of the specimen in a syringe-like manner.

Mechanically Integrated and Closely Coupled Print Head and Mist Source

A deposition apparatus comprising one or more atomizers structurally integrated with a deposition head. The entire head may be replaceable, and prefilled with material. The deposition head may comprise multiple nozzles. Also an apparatus for three dimensional materials deposition comprising a tiltable deposition head attached to a non-tiltable atomizer. Also methods and apparatuses for depositing different materials either simultaneously or sequentially.