Verwendung von Kohlendioxid, das als Nebenprodukt eines Methan-Dampfreformers anfällt, in einem Verfahren zur Herstellung von Biokraftstoffen aus Algen

Kohlendioxid, das als Nebenprodukt der Wasserstoffherstellung aus einem Methan-Dampfreformer (SMR) gesammelt wird, wird als Nahrungszufuhr für das Wachstum von Algenbiomasse für verschieden Zwecke, z. B. Biokraftstoffherstellung, chemische Zwischenprodukte, Nahrungsergänzungsmittel oder Pharmazeutika, verwendet. Diese Erfindung hilft dabei, Emissionen von Kohlendioxid, einem bekannten Treibhausgas, zu verringern und dabei, die Algenkultivierung zu optimieren, die für die Herstellung von Biokraftstoffen verwendet wird, um die Abhängigkeit von Erdöl und fossilen Brennstoffen zu verringern.

Sensoraufnahme für einen Bioreaktor sowie Bioreaktor mit Sensoraufnahme und Verfahren zur Vermehrung oder Kultivierung biologischen Materials

Bioreaktor umfassendeinen Behälter zur Aufnahme von fluiden Medien, die biologisches Material enthalten,eine Durchführung mit einer Durchgangsöffnung zwischen dem Inneren des Behälters zur Aufnahme von fluiden Medien, die biologisches Material enthalten,und dem Äußeren des Behälters zur Aufnahme von fluiden Medien, die biologisches Material enthalten, dadurch gekennzeichnet, dasssich eine Sensoraufnahme zum Halten von zumindest einem Sensor zumindest teilweise in der Durchgangsöffnung der Durchführung erstreckt, wobei die Sensoraufnahme mit deren Fenster die Durchgangsöffnung verschließt.

Mixed organic waste disposal by conversion to solid and gaseous fuels

Organic materials in the waste are separated from inorganics and metals (13, 14), before de-watering (3) and pelletization (6). Water released is fermented in reactors (4). Biogas produced is consumed in a power station (10), or is sent to a pressure reactor (5), for reforming with the pellets. An Independent claim is included for the corresponding plant.

Mikroalgen-Kohlenstofffixierungssystem mit Energieeinsparungs- und Luftreinigungsfunktion

Kohlenstofffixierungssystem mit einer Energieeinsparungsfunktion und einer Luftreinigungsfunktion, aufweisend: einen Grundsockel (20), der eine Steuervorrichtung (21), eine Lufteintrittspumpe (25) und eine Batterie (22) aufweist, wobei die Lufteintrittspumpe (25) und die Batterie (22) elektrisch mit der Steuervorrichtung (21) verbunden sind; einen Hauptstamm (30), der mit dem Grundsockel (20) verbundenen ist; eine Züchtungsvorrichtung (40), die der Kohlenstofffixierung dient und rings um dem Hauptstamm (30) angeordnet ist, wobei die Züchtungsvorrichtung (40) ein Züchtungsrohr (41) und eine schwimmend angeordnete Lichtquelle (42) aufweist, und wobei im Züchtungsrohr (41), das kopfseitig eine Reihe Belüftungslöcher (411) und bodenseitig ein Mehrwegeventil (44) aufweist, eine aus einer Mikroalgenflüssigkeit und einer Nährlösung zusammengesetzte Flüssigkeit enthalten ist, und wobei das Mehrwegeventil (44) über eine Rohrleitung (45) mit der Lufteintrittspumpe (25) verbunden ist, wobei die Lufteintrittspumpe ...

Solar-assisted volatile fermentation products production processes

SOLAR-ASSISTED VOLATILE FERMENTATION PRODUCTS PRODUCTION PROCESSES

Solar-assisted volatile fermentation products production processes

Biogas generator

Biogas generator

Biogas generator

Solar-assisted volatile fermentation products production processes

PROCEDURE FOR PRODUCING METHANHALTIGEM FERMENTATION GAS FROM ORGANIC MATERIALS.

Process and apparatus for the production of alcohols

A process for the production of C2+ alcohols from a methane-containing feedstock which process comprises a) optionally reacting in a pre-reformer the methane-containing feedstock in the presence of steam, where the steam plus C0 ...

Electromagnetic bioaccelerator

Methods and systems for the production of hydrocarbon products

Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from a CO2 reforming process.

Methane Production System of Methane Tank

The present invention provides a methane production system of a methane tank, which belongs to methane technique field. It solves technique problems such as low gas production efficiency, inconvenience of collecting gas and large space occupied by the storage device, that exist in the prior production system of methane. The present methane production system of the methane tank includes a methane tank. The methane tank includes a tank body that can produce methane, a cover and a connecting pipe pivotally connected to an upper part of the tank body. An electric heating pipe is set in the tank body. An end of the connecting pipe extends through the cover and is in communication with the tank body. The connecting pipe and the cover keep airtight. Other end of the connecting pipe is connected to a storage device. A control mechanism that automatically turns on the electric heating pipe when a temperature in the tank body is smaller than a set value is set in the tank body. The present invention ...

INTEGRATED FERMENTATION AND ELECTROLYSIS PROCESS

INTEGRATED FERMENTATION AND ELECTROLYSIS PROCESS

The invention provides schemes for the integration of a fermentation process, with an electrolysis process, and a C1-generating industrial process. In particular, the invention provides process for utilizing electrolysis products, for example H2 and/or O2, to improve the process efficiency of at least one of the fermentation process or the C1-generating industrial process. More particularly, the invention provides a process whereby, H2 generated by electrolysis is used to improve the substrate efficiency for a fermentation process, and the O2 generated by the electrolysis process is used to improve the composition of the C1-containing tail gas generated by the C1-generating industrial process.

INTEGRATED FERMENTATION AND ELECTROLYSIS PROCESS

The invention provides schemes for the integration of a fermentation process, with an electrolysis process, and a C1-generating industrial process. In particular, the invention provides process for utilizing electrolysis products, for example H2 and/or O2, to improve the process efficiency of at least one of the fermentation process or the C1-generating industrial process. More particularly, the invention provides a process whereby, H2 generated by electrolysis is used to improve the substrate efficiency for a fermentation process, and the O2 generated by the electrolysis process is used to improve the composition of the C1-containing tail gas generated by the C1-generating industrial process.

SYSTEMS AND METHODS FOR STILLAGE FRACTIONATION

Systems and methods for fractionating whole stillage from an ethanol production facility are provided. Whole stillage undergoes a separation of its liquid portion (thin stillage) from the solid portion (fiber cake). In some embodiments, the solids and liquids in whole stillage may be separated utilizing a screening centrifuge. The fiber cake may be dried to generate a high fiber animal feed. The thin stillage may be provided to a three-phase separator for separation into an oil emulsion, an aqueous clarified stillage, and a protein paste. The protein paste may be dried to generate a high protein animal feed with greater than about 45% protein content. The clarified thin stillage is condensed to yield a syrup with greater than around 60% solids. The oil emulsion is subjected to a pH adjustment to liberate the oil from the emulsion, which is then separated.

SYSTEMS AND METHODS FOR STILLAGE FRACTIONATION

Systems and methods for fractionating whole stillage from an ethanol production facility are provided. Whole stillage undergoes a separation of its liquid portion (thin stillage) from the solid portion (fiber cake). In some embodiments, the solids and liquids in whole stillage may be separated utilizing a screening centrifuge. The fiber cake may be dried to generate a high fiber animal feed. The thin stillage may be provided to a three-phase separator for separation into an oil emulsion, an aqueous clarified stillage, and a protein paste. The protein paste may be dried to generate a high protein animal feed with greater than about 45% protein content. The clarified thin stillage is condensed to yield a syrup with greater than around 60% solids. The oil emulsion is subjected to a pH adjustment to liberate the oil from the emulsion, which is then separated.

PURIFICATION OF BIOLOGICAL MOLECULES

The present invention relates to improved processes and systems for purification of biological molecules, where the processes can be performed in a continuous manner.

SOLID GEL AMPLIFICATION METHOD AND APPARATUS FOR PLATFORM MOLECULAR DIAGNOSTICS

The present invention provides for a novel system and method for amplification and detection of nucleic acids within a miniaturized device wherein sample administration occurs via capillary forces through a channel created by drying a hydrogel containing all components needed for a cell-free, enzymatic, nucleic-acid amplification system other than the template nucleic acid or precursor thereto, and wherein an aqueous sample is provided to the desiccated hydrogel, and the hydrogel is rehydrated, through capillary forces.

INTEGRATED FERMENTATION PROCESS AND ELECTROLYSIS

PERFUSION BIOREACTOR AND ASSOCIATED METHODS OF USING

METHOD AND SYSTEM FOR PRODUCING ALCOHOLS AND/OR ACIDS

METHOD AND SYSTEM FOR PRODUCING HYDROCARBON PRODUCTS

METHOD FOR THE PRODUCTION OF HYDROCARBON PRODUCTS

Centralized treatment device for fermentation tail gases, and application method thereof

Novel cold-region biogas digester

Annually constant and stable methane production system by solar heating

Movable type solar high-temperature high-efficiency methane generator device

The invention relates to a movable type solar energy high-temperature methane generator device comprising a solar energy heat collector, a methane generator, a heat transfer pipe, a pressure gauge, adehydrator, a desulfurizer, a vacuum pump, an air storage tank, gas equipment, an exhaust pipe, a pulp extraction pump, a pressure sensor A, a pressure sensor B, a feed hole, a discharge hole, a firsttemperature inductor, a hot water pump, a second temperature inductor, a first pressure sensor, a second pressure sensor, a pressure valve and an integral device, wherein the integral device is formed by connecting the ground and a heat preservation material. The movable type solar high-temperature methane generator device is characterized by also comprising a heat energy transmission and temperature control and preservation system with heat energy generated by solar energy, a methane automatic storage system with methane generated by the methane generator, a subsided material system with subsided ...

Device for manufacturing organic substance and method for manufacturing organic substance

Solar auxiliary heating methane production device

The invention discloses a solar auxiliary heating methane production device. The device comprises a biomass methane fermentation system, wherein the biomass methane fermentation system comprises an anaerobic reaction box and a gas storage tank which is arranged on the upper part of the anaerobic reaction box and provided with a gas outlet; the bottom of the gas storage tank is provided with a vent hole communicated with the anaerobic reaction box; the anaerobic reaction box is provided with a feeding hole, a discharging hole, a feeding hole valve and a discharging hole valve; a feeding pump is arranged between the feeding hole valve and the anaerobic reaction box; the biomass methane fermentation system is connected with an auxiliary heating system which comprises a heat exchanger and a heat collector; the heat exchanger is arranged at the bottom of the anaerobic reaction box; the middle part of the inner side of the anaerobic reaction box is provided with at least one baffle plate and ...

Food drying and fresh-keeping integrated device

The invention discloses a food drying and fresh-keeping integrated device. By means of the device, organic substances of straws, bad food, feces and the like enter into a biological energy production area to be treated to produce biological energy and resources such as methane, organic fertilizer and biogas slurry fertilizer. By means of an intermittent type food fresh-keeping drying room, fruits and vegetables which are recently picked can be subjected to fresh-keeping and fruits and vegetables which are not sold can be subjected to drying treatment. The food fresh-keeping mode of the intermittent type food fresh-keeping drying room includes a plurality of food fresh-keeping modules are used for keeping food fresh by methane application, and the temperature maintenance is controlled based on the characteristic that the underground water is warm in winter and cool in summer. The food drying of the intermittent type food fresh-keeping drying room utilizes a double-heat-source of the solar ...

PROCESS FOR THE METHANATION AND METHANATION COMPRISING A STEP OF SEPARATING AND RECIRCULATING HYDROGEN AND INSTALLATION FOR IMPLEMENTING SAID METHOD PROCESS

METHOD FOR PRODUCING AN ORGANIC PRODUCT FROM A FEEDSTOCK OF CARBONACEOUS MATERIAL USING A GASIFICATION FOLLOWED BY FERMENTATION OF THE SYNTHESIS GAS.

L'invention concerne un procédé de production d'un carburant, notamment un carburant liquide, ou d'un autre produit organique, à partir d'une charge de matière carbonée, comprenant les étapes suivantes : a/ gazéification de la charge de matière carbonée dans un premier réacteur, dit gazéifieur (1), b/ en aval de la gazéification, fermentation du gaz de synthèse produit selon l'étape a/, au moyen de micro-organismes, de l'eau et des nutriments dans un deuxième réacteur, dit fermenteur (2), c/ récupération, en aval du fermenteur, des micro-organismes et de l'eau ; d/ injection d'au moins une partie des micro-organismes et le cas échéant d'au moins une partie de l'eau récupérés en entrée (10) du gazéifieur.

PROCESS FOR MANUFACTURING POLYLACTIC ACID, APPARATUS OF PRODUCING LACTIC ACID AND APPARATUS FOR MANUFACTURING POLYLACTIC ACID

PURPOSE: A process for manufacturing polylactic acid is provided to obtain polylactic acid with high efficiency in short term by generating lactic acid from vegetable starch and lactobacillus through fermentation and dehydration polycondensing the lactic acid to synthesize polylactic acid. CONSTITUTION: The process comprises the steps of: polymerizing a lactic acid polymer by heating and dehydration polycondensing lactic acid; and electrically dehydrating the polymerized material by passing direct current to the lactic acid polymer and migrating water portion of the polymer to a dehydration part of an electric dehydration tank by electric penetration. The dehydration tank includes a pair of electrodes(32,34), a compartment(36) and a dehydration part isolated by the compartment. The dehydration part is mounted on a cathode of the electrodes. The process is carried out by using an apparatus which has a lactic acid feeder, a polymerizing device and an electric dehydration device. © KIPO 2007 ...

BIOENERGY PRODUCING SYSTEM USING MARINE BIOMASS BY CONVERTING THE BIOMASS INTO METHANOL AND ETHANOL

PURPOSE: A bioenergy producing system using marine biomass is provided to produce ethanol through a fermentation process and to enhance the conversion efficiency from waste to bioenergy. CONSTITUTION: A bioenergy producing system using marine biomass comprises a fermentation process(10) for producing ethanol from the marine biomass and a gasification process(20) by collecting organic waste generated during an ethanol production process to obtain methanol. The fermentation system comprises a pre-treatment device(11) for pre-treating the marine biomass, and a fermentation device(13) for fermenting the marine biomass and producing ethanol. The gasification system comprises: an organic waste collector(21), a gasifier(23), a reformer(25), and a methanol synthetic reactor(27). COPYRIGHT KIPO 2012 ...

SYSTEM FOR PROCESSING OF HIGH VALUE PRODUCTS CO2 IN AGGREGATE AND/OR NUTRITIONAL AND OTHER FEATURES OF ENERGY

PHOTOBIOREACTOR SYSTEM FOR HIGH-DENSITY CULTURE OF MICROALGAE

The invention discloses a photobioreactor system for high-density culture of microalgae which includes photobioreactor, and also includes solar collector, optical fiber, and illuminant device within the photobioreactor, residue gas absorption device, and medium separating recovery respectively connected with the photobioreactor. One end of the illuminant device is connected with spectrophotometric adjustment device, which is on the photobioreactor and connected with solar collector through optical fibers. Between the lower of the illuminant device and the bottom of the photobioreactor, there has a gas distributor, which is connected with the output of the gas mixing device. The system can increase the utilization efficiency of solar; reduce the consuming of external electrical energy. The system resolves the problem of sunlight using, such as intermittence, instability, and hard collection, so insures continually and stably culturing microalgae.

MICROBIAL ALKANES FROM C-1 SUBSTRATE

The disclosure relates to biological methods of making a hydrocarbon feedstock wherein carbon substrates are converted into useful chemicals and fuels. Particularly, genetically engineered bacteria are used to make C4-C10 fatty acids or derivatives from one-carbon substrates such as methanol and carbon dioxide.

BIOPROCESS AND MICROBE ENGINEERING FOR TOTAL CARBON UTILIZATION IN BIOFUELPRODUCTION

Some aspects of this invention provide methods and bioreactors for converting a carbon source into a lipid. In some embodiments, lipid production is carried out in an aerobic fermentor and carbon dioxide generated during lipid production is converted into a carbon substrate by CO2 fixation in an anaerobic fermentor. In some embodiments, the carbon substrate generated by CO2 fixation is used as the carbon source for lipid production, thus achieving total carbon utilization in lipid production.

IMAGING APPARATUS FOR EXPERIMENTAL USE, EXPERIMENTAL SYSTEM, AND CONTROL METHOD OF EXPERIMENTAL SYSTEM

An experimental system includes an imaging unit which generates image data by imaging a sample, and an observation moving mechanism which changes a position of the imaging unit. The system also includes a supply and removal unit which supplies a liquid to the sample or removes the liquid from the sample, an operation moving mechanism which changes a position of the supply and removal unit, and a pump which moves the liquid through the supply and removal unit. Furthermore, the system includes a control circuit which controls operations of the observation moving mechanism and the operation moving mechanism such that the positions of the imaging unit and the supply and removal unit change together, and which controls an operation of the pump in accordance with the positions of the imaging unit and the supply and removal unit. 1. An imaging apparatus for experimental use , which is movable to different positions in a specific plane in which a sample is distributed , and which enables an imaging position to be changed in accordance with a position of an external substance operation mechanism configured to add or remove a substance , the imaging apparatus comprising:an imaging unit which generates image data by imaging the sample;an observation moving mechanism which changes a position of the imaging unit; anda control circuit which controls an operation of the observation moving mechanism such that the position of the imaging unit and a substance operation position of the substance operation mechanism change together.2. The imaging apparatus according to claim 1 , wherein the control circuit extracts a signal relating to the position of the imaging unit from control signals including the signal relating to the position of the imaging unit and a signal relating to the substance operation position claim 1 , and controls the operation of the observation moving mechanism.3. The imaging apparatus according to claim 1 , wherein the control circuit controls the operation of the ...

Process and Apparatus for Assisting the Extraction and Processing of Biodiesel Oil Using Oil-Bearing and Other Organic Feedstock

A process and apparatus for assisting the extraction and processing of biodiesel oil from organic feedstock includes: providing crushed oil-bearing organic feedstock meal from which has been extracted a first amount of biodiesel oil wherein the meal retains a second amount of entrained oil; forming a meal slurry containing said meal and passing the slurry to an anaerobic digester; anaerobically digesting the meal slurry so as to convert said second amount of entrained oil to produce heat, methane gas, and organic fertilizer or oil-free cattle feed; providing an electrical generator and employing the methane gas for at least the production of electricity by burning the methane gas in the electrical generator which is adapted to convert heat to electricity and re-cycling at least some of the electricity.

A METHOD FOR DEVELOPMENTALLY ACTIVATING A CELL

The claimed invention is directed towards equipment, methods and compositions involving application of an Electric field that are suitable for in vivo electroporation, in vitro application of an Electric field and the generation of developmentally-activated, totipotent, pluripotent, pluripotent-like, multipotent, and/or self-renewing cells which are capable of beginning to differentiate in culture into a variety of cell types and capable of further differentiation in vivo. The claimed invention is also directed towards the generation of desirable, differentiating somatic cell populations transplantable to animals or patients, to drug screening and drug discovery, cellular therapy, immunotherapy, gene therapy, tissue engineering, and the treatment of patients suffering from diseases that may be ameliorated by these methods. This invention also provides methods for preventing, treating, or retarding disease, for example, immunodeficiency virus (e.g. HIV-1, HIV-2, SIV, FIV, etc.) infection.

ENERGY PHOTOCONVERTER FOR OBTAINING BIOFUELS

The present invention relates to the design of energy photoconverters which act in a continuous and closed manner for producing biofuels and other products of interest by means of the mass culturing of phytoplankton.

METHOD FOR PRODUCING SOLID MATERIALS FROM PLANT MATERIAL, MATERIAL PRODUCED ACCORDING TO SAID METHOD, USE OF THE MATERIAL IN ADDITION TO A SYSTEM FOR CARRYING OUT SAID METHOD

The invention relates to a method for producing solid materials, particularly building materials, wherein vegetal source material is ground, fermented and at least partially dried. The vegetal source material is ground into fine grains, fermented by adding microorganisms, preferably yeast, and dried. Non-fermenting parts of the vegetal source material are bonded when drying by substances from the microorganisms, so that a solid ecologically safe material can be produced without conventional adhesives.

АВТОМАТИЗИРОВАННЫЕ СПОСОБЫ ОБРАБОТКИ КЛЕТОК, МОДУЛИ, ИНСТРУМЕНТЫ И СИСТЕМЫ

СПОСОБ ПОЛУЧЕНИЯ ТВЕРДЫХ МАТЕРИАЛОВ ИЗ РАСТИТЕЛЬНОГО МАТЕРИАЛА, ПОЛУЧЕННЫЙ ЭТИМ СПОСОБОМ КОНСТРУКТИВНЫЙ МАТЕРИАЛ, ПРИМЕНЕНИЕ МАТЕРИАЛА И УСТАНОВКА ДЛЯ ОСУЩЕСТВЛЕНИЯ СПОСОБА

... 1. Способ получения твердых материалов, в частности строительных материалов, причем растительный исходный материал измельчается, ферментируется и, по меньшей мере, частично сушится, при этом неферментированные части растительного исходного материала склеиваются выделяемыми микроорганизмами веществами, отличающийся тем, что проводятся ферментация добавкой микроорганизмов и сушка, что время брожения составляет менее 72 часов и что твердый материал после сушки является пористым. 2. Способ по п.1, отличающийся тем, что растительным исходным материалом является древесина, солома или их смесь. 3. Способ по п.1 или 2, отличающийся тем, что ферментация проводится с помощью соответствующих грибков, преимущественно дрожжей или бактерий. 4. Способ по п.3, отличающийся тем, что дрожжи выращиваются в результате добавки стимулирующих рост веществ, в частности, крахмала или древесного сахара, на измельченной на тонкие зерна древесине и что древесина ферментируется этими выращенными дрожжами. 5. Способ ...

Processing of animal cadaver(s) to yield bio-gas and liq. fertilizer

Animal cadavers are processed by: (a) successive stages of comminution; (b) decomposition pretreatment; (c) hydrolysis at 18-40 deg C to give a hydrolysate with a pH of 3-5; (d) methanisation at 50-60 deg C and a pH of 6.5-8.5 and subsequent (e) sterilisation, to yield biogas and a liq. fertilizer. Also claimed is the use of a specified modular appts. for carrying out the process.

Anti-anemia drug cyanocobalamin production plant

Methods and systems for the production of hydrocarbon products

Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.

Methane Production System of Methane Tank Having Improved Structure

The present invention provides a methane production system of a methane tank having improved structure, which belongs to methane technique field. It solves technique problems such as low gas production efficiency, inconvenience of collecting gas and large space occupied by the storage device, that exist in the prior production system of methane. The present methane production system of the methane tank having improved structure includes a methane tank. The methane tank includes a tank body that can produce methane, a cover and a connecting pipe pivotedly connected to an upper part of the tank body. An electric heating pipe is set in the tank body. An end of the connecting pipe extends through the cover and is in communication with the tank body. The connecting pipe and the cover keep airtight. Other end of the connecting pipe is connected to a storage device. A control mechanism that automatically turns on the electric heating pipe when a temperature in the tank body is smaller than a set ...

INTEGRATION OF FERMENTATION AND GASIFICATION

The invention provides for the integration of a gas fermentation process with a gasification process whereby effluent downstream from the gas fermentation process is recycled to the gasification process. The invention is capable of recycling one or more effluents including biogas generated from a wastewater treatment process, tail-gas generated from the fermentation process, unused syngas generated by the gasification process, microbial biomass generated from the fermentation process, microbial biomass generated from a wastewater treatment process, crude ethanol from the product recovery process, fusel oil from the product recovery process, microbial biomass depleted water, wastewater generated from the fermentation process, and clarified water from a wastewater treatment process to a gasification process.

CELL CULTURE CLARIFICATION

A method and a kit for cell culture clarification, as well as a system or apparatus for clarifying a cell culture and purifying biomolecules of interest, are described. The cell culture comprises at least one biomolecule of interest. The method comprises adding one or more compounds to the cell culture that allow the formation of floccules of solutes and/or particulates in said cell culture harvest, adding an amount of diatomaceous earth (DE) to said cell culture harvest, agitating the obtained solution, and transferring said solution to a filtration vessel comprising a support filter having a surface, whereby the diatomaceous earth in said solution forms a layer on said surface and filtering said harvest solution through said layer and support filter.

METHOD FOR PRODUCING ORGANIC MOLECULES FROM FERMENTABLE BIOMASS

Le procédé de production de molécules organiques à partir de biomasse fermentescible, comprend une étape de fermentation (5) anaérobie produisant des acides gras volatils (6), ces précurseurs étant transformés en molécules organiques finales par voie non fermentaire. Il comprend également au moins les étapes suivantes; a) extraire (9) au moins une partie des acides gras volatils du milieu de fermentation de sorte que la production de métabolites fermentaires par les microorganismes (M) n'est pas affectée et introduire une partie de la phase liquide (11) contenant des microorganismes issue de l'extraction (9), b) synthétiser (13) des molécules organiques à partir des métabolites fermentaires ou des acides gras volatils extraits à l'étape a), c) poursuivre les étapes a) à b) jusqu'à l'obtention, en quantité et en qualité, des molécules organiques finales. L'invention concerne également une installation de mise en uvre du procédé.

METHODS AND SYSTEMS FOR THE PRODUCTION OF HYDROCARBON PRODUCTS

Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from a CO2 reforming process.

METHODS AND SYSTEMS FOR THE PRODUCTION OF HYDROCARBON PRODUCTS

Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.

METHODS AND SYSTEMS FOR THE PRODUCTION OF ALCOHOLS AND/OR ACIDS

METHOD AND SYSTEM FOR PRODUCING HYDROCARBON PRODUCTS

Full water freezen protection system of solar biogas generating pit in sharp frost area

An all-water anti-freezing system for a solar biogas digester in a severe cold area adopts water as a heat exchange medium to solve the problems of prior anti-freezing system for the solar biogas digester such as high cost, poor heat exchange effect, high energy consumption, and inconvenience in generalization in the rural areas. In the invention, the water outlet of a circulating pump is connected with the water inlet of a solar energy collector; a water check valve is arranged on a water circulating pipe between the water outlet of the circulating pump and the water inlet of the solar energy collector; the water outlet of the solar energy collector and the water outlet of a water supply pump are connected with the water inlet of a heating coil; the water inlet of the circulating pump and the water inlet of a drain pump are connected with the outer outlet of the heating coil; the water inlet of the water supply pump is connected with the water outlet of a water storage tank; and the water ...

Solar methane device

A solar methane device comprises a methane-generating pit, a solar thermal collector, an upper box body, an evacuated solar collector tubes, a feeding port, a discharging port, an observing window, a temperature detecting device, a lower box body, a heat-emitting element, a power line and a methane delivery tube. The solar methane device has the advantages and active effects that the device is simple in structure and convenient to install, measures the temperature and controls the temperature of the methane-generating pit through the temperature detecting device and plays an energy-saving role, and simultaneously in cloudy days lasting for a long period of time, the methane-generating pit can be heated through mains supply, continuous work of the methane-generating pit can be ensured, the work efficiency of the methane-generating pit can be improved, and energy can be saved.

Temperature controlled integrated application system for renewable energy source combination device and agricultural production

Methane tank with solar thermal power generation and wind power generation in complementary mode to increase gas production rate

High-concentration horizontal type self-slagging biogas generation tank

Methane preheating device

Solar spherical methane tank

Sustainable economic development through integrated production of renewable energy, materials resources, and nutrient regimes

INSTALLATION DU TYPE DIGESTEUR POUR LA PRODUCTION DE METHANE

L'INVENTION CONCERNE UNE INSTALLATION POUR LA PRODUCTION DE METHANE PAR DIGESTION ANAEROBIE DE MATIERES ORGANIQUES. ELLE COMPORTE UNE FOSSE DE DIGESTION ANAEROBIE EN FORME DE COULOIR 7 ALIMENTEE EN CONTINU EN AMONT 6 A PARTIR D'UNE FOSSE DE PREDIGESTION 1.LA MATIERE EN TRAITEMENT S'ECOULE DANS LE COULOIT 7 DE FACON CONTINUE PENDANT QUE SE POURSUIT SA DEGRADATION BIOLOGIQUE. DES POMPES DE RECYCLAGE ASSURENT UN BRASSAGE DE LA MATIERE DANS LE COULOIR QUI SE PRESENTE AVANTAGEUSEMENT SOUS LA FORME D'UN LONG BOYAU EN MATERIAU PLASTIQUE.

METHOD FOR PRODUCING MOLECULES FROM BIOMASS FERMENTABLE

Le procédé de production de molécules organiques à partir de biomasse fermentescible, comprend une étape de fermentation (5) anaérobie produisant des acides gras volatils (6), ces précurseurs étant transformés en molécules organiques finales par voie non fermentaire. Il comprend également au moins les étapes suivantes : -a) extraire (9) au moins une partie des acides gras volatils du milieu de fermentation de sorte que la production de métabolites fermentaires par les microorganismes (M) n'est pas affectée, - b) synthétiser (13) des molécules organiques à partir des métabolites fermentaires ou des acides gras volatils extraits à l'étape a), - c) poursuivre les étapes a) à b) jusqu'à l'obtention, en quantité et en qualité, des molécules organiques finales. L'invention concerne également une installation de mise en oeuvre du procédé.

CULTURE SYSTEM PHOTOSYNTHETIC MICROORGANISMS WITH IMPROVED EFFICIENCY

bioreactor perfusion and related methods of use

aparelhagem para a produção da solução de sacarídeo utilizando biomassa como matéria-prima de adução

METHODS AND SYSTEMS FOR THE PRODUCTION OF HYDROCARBON PRODUCTS

Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from a CO2 reforming process.

MICROALGAE-BASED SOIL INOCULATING SYSTEM AND METHODS OF USE

A system for inoculating soil with microalgae is provided. The system is portable and can be used with many different water sources to culture microalgae and form an inoculate that is added to irrigation water used for watering crops. The system provides improved crop production metrics as compared to crops grown without the microalgae-based inoculation system. The system can be integrated into existing irrigation systems to add macronutrients and micronutrients into the water, thereby providing highly bioavailable nutrients to crops.

CONTINUOUS SYSTEM FOR CONVERTING CO2 INTO PRODUCTS WITH HIGH ADDED AND/OR NUTRITIONAL VALUE AND OTHER ENERGY RESOURCES

The invention relates to a system for generating products with high added or nutritional value and other energy resources, comprising first means for the cultivation of at least one phytoplankton culture and natural and/or artificial means for illuminating the phytoplankton culture in order to promote the growth and/or multiplication thereof. According to the invention, the phytoplankton culture can be illuminated both by external light sources and internal light sources independent of the external light sources.

SYSTEMS FOR THE UTILIZATION OF RUMINANT ANIMAL METHANE EMISSIONS

A process for the utilization of the methane produced by enteric fermentation, specifically to a process that utilizes methane produced by ruminant animals through enteric fermentation as a source of carbon and/or energy for the directed production of methane-based goods or processes is provided.

PROCESSING BIOMASS USING THERMOCHEMICAL PROCESSING AND ANAEROBIC DIGESTION IN COMBINATION

Systems and methods for integrating thermochemical processing of biomass and anaerobic digestion are provided. Light oxygenated organic compounds are produced as byproducts of thermochemical biomass processing e.g. by torrefaction and/or pyrolysis, and are converted to methane by anaerobic digestion. Thermochemical processing units may or may not be co-located with the anaerobic digestion units, with co-location providing benefits for e.g. rural agricultural enterprises.

Methods and systems for the production of hydrocarbon products

Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.

INTEGRATED PROCESSING PLANTS

An integrated plant comprising: (a) one or more lignocellulose processing units producing one or more sugar streams and one or more lignin streams; (b) one or more lignin-processing units processing one or more of said lignin streams into a lignin product; and (c) one or more sugar processing units processing one or more of said sugar streams into a sugar product; (d) at least one transfer mechanism transferring one or more of said sugar stream(s) to one or more of said sugar processing units over a distance of 5 km or less; and (e) at least one transfer mechanism transferring said lignin stream from one or more of said lignocellulose processing units to one or more of said lignin processing units over a distance of 5 km or less.

Systems and methods for contaminant removal from a microalgae culture

Systems and methods for the removal of contaminants from a liquid culture microalgae and/or cyanobacteria comprise an let tube, a pump, a gas injector, a vertical chamber, and/or a collection container that promotes the production of foam in the microalgae culture, wherein the foam contains the contaminants.

СПОСОБ И АППАРАТ ДЛЯ УДАЛЕНИЯ ДИОКСИДА УГЛЕРОДА (СО) ИЗ ПОТОКА ГАЗООБРАЗНЫХ ВЕЩЕСТВ

Группа изобретений относится к области биохимии. Предложен способ снижения количества COв потоке газообразных веществ, а также аппарат для удаления COиз потока газообразных веществ. Способ включает контактирование газообразных веществ, содержащих CO, с первым потоком водной поглощающей жидкости, содержащей карбонат ангидразу, поглощение COуказанной жидкостью и превращение его в более растворимый неорганический углерод. Осуществление разделения первого потока жидкости на второй и третий потоки жидкости, где второй поток содержит более высокую концентрацию карбонат ангидразы относительно третьего потока. Осуществление контактирования третьего потока жидкости с микроорганизмом, способным превращать растворенный в жидкости неорганический углерод в кислород и/или биомассу. Аппарат содержит первую и вторую системы циркуляции текучего вещества. Первая система содержит поглощающий блок с внутренним пространством, ниже по потоку от поглощающего блока - фильтровальный блок с фильтром. Вторая система ...

СПОСОБ И УСТРОЙСТВО ДЛЯ ПОЛУЧЕНИЯ СПИРТОВ

Группа изобретений относится к области биохимии. Предложены способ получения С-Сспиртов из метансодержащего сырья, оборудование для получения С-Сспиртов и способ получения топлива для моторизованного транспорта. Способ получения С-Сспиртов включает риформинг метансодержащего сырья в первом риформере с получением первого потока продукта, включающего CO, Hи CO. Риформинг части первого потока продукта и/или части метансодержащего сырья во втором риформере в присутствии пара и кислорода с получением второго потока продукта, включающего CO, Hи CO. Бактериальную ферментацию с использованием ацетогенных анаэробных бактерий первого и второго потоков продукта с получением третьего потока продукта, включающего водный раствор С-Сспирта, питательные вещества и промежуточные продукты реакции, а также четвертого потока продукта, включающего СО, Ни CO. Возврат части четвертого потока продукта в метансодержащее сырье и выделение части С-Сспирта из третьего потока продукта с получением пятого потока продукта ...

УСТРОЙСТВО ДЛЯ ПОЛУЧЕНИЯ УГЛЕРОДА С ИСПОЛЬЗОВАНИЕМ БИОМАССЫ

Изобретение относится к области контроля окружающей среды, связанного с обработкой сточных вод, обессериванием дымовых газов, переработкой мусора и ликвидацией бытовых отходов, а также с областью производства сажи, метанола, диметилового эфира и бензина. Устройство для получения углерода путем обработки биомассы включает блок анаэробной ферментации для образования метана и диокида углерода путем анаэробной ферментации биомассы; блок сепарации метана - диоксида углерода для отделения метана и диоксида углерода из смешанного газа, содержащего метан и диоксид углерода, образованного в указанном блоке анаэробной ферментации; средства образования водорода для образования водорода из указанного метана, выделенного в указанном блоке сепарации метана - диоксида углерода; и блок связывания диоксида углерода для образования углерода путем восстановления указанного диоксида углерода, выделенного в указанном блоке сепарации метана - диоксида углерода, при помощи указанного водорода, образованного в ...

АВТОМАТИЗИРОВАННЫЕ СПОСОБЫ ОБРАБОТКИ КЛЕТОК, МОДУЛИ, ИНСТРУМЕНТЫ И СИСТЕМЫ

СПОСОБ ПОЛУЧЕНИЯ ОРГАНИЧЕСКИХ МОЛЕКУЛ ИЗ СБРАЖИВАЕМОЙ БИОМАССЫ

Plant useful for using carbon dioxide from different sources, accumulating in temporally variable manner, for ecological energy, comprises e.g. device for producing hydrogen, and device for removing carbon dioxide-containing gases

Plant for using carbon dioxide from different sources, accumulating in a temporally variable manner, for ecological energy, comprises at least one device for producing hydrogen using solar energy, at least one device for removing carbon dioxide-containing gases (8) releasing continuously from emission sources, an intermediate storage (12) for storing carbon dioxide releasing discontinuously from emission sources, at least one device for treating carbon dioxide-containing gases of different emission source, at least one methanation device (13) and at least one device for further use of methane. Plant for using carbon dioxide from different sources, accumulating in a temporally variable manner, for ecological energy, comprises (i) at least one device for producing hydrogen using solar energy, (ii) at least one device for removing carbon dioxide-containing gases (8) releasing continuously from emission sources, (iii) an intermediate storage (12) for storing carbon dioxide releasing discontinuously ...

Apparatus and process for treating waste

Alkanol

Global photosynthesising sites to replace fossil fuels and solve the concomitant negative global climate change, environmental pollution

Demineralisation of organic tissue

A method of processing a mineralised organic tissue feedstock comprises enzymatic hydrolysis to produce hydrolysis fractions and mineralised tissue remnants, separating the mineralised tissue remnants from the hydrolysis fractions, and demineralising the mineralised tissue remnants to produce solubilised minerals and demineralised tissue remnants. Demineralising may comprise treating with acid, e.g. HCl, and may take place in a rotating drum reactor. A method of demineralising mineralised tissues is also claimed, comprising mixing mineralised tissues with an acid solution to solubilise the minerals. The mixing occurs in a rotating drum reactor (see figures 6-9) comprising a drum rotatable about its longitudinal axis, an inlet and an outlet provided at respective first and second points on the drum, and a helical screw-blade fixed to the interior of the drum for conveying and mixing the tissues and acid as the drum rotates. A processing plant for processing an organic feedstock comprising ...

A method for development ally-activating a cell

The claimed invention is directed towards equipment, methods and compositions involving application of an Electric field that are suitable for in vivo electroporation, in vitro application of an Electric field and the generation of developmentally-activated, totipotent, pluripotent, pluripotent-like, multipotent, and/or self-renewing cells which are capable of beginning to differentiate in culture into a variety of cell types and capable of further differentiation in vivo. The claimed invention is also directed towards the generation of desirable, differentiating somatic cell populations transplantable to animals or patients, to drug screening and drug discovery, cellular therapy, immunotherapy, gene therapy, tissue engineering, and the treatment of patients suffering from diseases that may be ameliorated by these methods. This invention also provides methods for preventing, treating, or retarding disease, for example, immunodeficiency virus (e.g. HIV-1, HIV-2, SIV, FIV, etc.) infection ...

PROCEDURE AND DEVICE FOR THE PROCESSING OF RESIDUAL SUBSTANCE MIXTURES AND FOR THE CONVERSION OF CARBON-CONTAINING RESTODER RAW MATERIALS IN THE RESIDUAL SUBSTANCE MIXTURES

DEVICE FOR USING LIQUID WASTES

Ethanol production from biological wastes

Perfusion bioreactor and related methods of use

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

Methane Production System of Methane Tank

The present invention provides a methane production system of a methane tank, which belongs to methane technique field. It solves technique problems such as low gas production efficiency, inconvenience of collecting gas and large space occupied by the storage device, that exist in the prior production system of methane. The present methane production system of the methane tank includes a methane tank. The methane tank includes a tank body that can produce methane, a cover and a connecting pipe pivotally connected to an upper part of the tank body. An electric heating pipe is set in the tank body. An end of the connecting pipe extends through the cover and is in communication with the tank body. The connecting pipe and the cover keep airtight. Other end of the connecting pipe is connected to a storage device. A control mechanism that automatically turns on the electric heating pipe when a temperature in the tank body is smaller than a set value is set in the tank body. The present invention ...

PERFUSION BIOREACTOR AND RELATED METHODS OF USE

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

BIO-METHANOL PRODUCTION

Methods and systems for producing bio-methanol can include anaerobic digestion of a biomass feedstock to produce biogas including methane and carbon dioxide, partial oxidation of the biogas with oxygen from water electrolysis to produce syngas, synthesizing bio-methanol from the syngas and hydrogen from the water electrolysis, storing the bio-methanol, intermittently using battery based electricity to power the electrolysis during peak electricity demand, and intermittently using renewable electricity from another source during off-peak demand. Electricity can also optionally be obtained by periodically combusting a portion of the bio-methanol. The techniques provide a route for the production of bio-methanol without the engagement of fossil fuels as feedstocks and mitigating fossil fuel derived greenhouse gas emissions from processing and utilization of transportation fuels and commercial or industrial alcohols.

Energy photoconverter for obtaining biofuels

The present invention relates to the design of energy photoconverters which act in a continuous and closed manner for producing biofuels and other products of interest by means of the mass culturing of phytoplankton.

Microbially-assisted water electrolysis for improving biomethane production

A method of producing in a bioreactor a biogas rich in methane involves electrolyzing water in an aqueous medium at a voltage in a range of from 1.8 V to 12 V in the presence of electrochemically active anaerobic microorganisms that biocatalyze production of hydrogen gas, and, contacting a species of hydrogenotrophic methanogenic microorganisms with the hydrogen gas and carbon dioxide to produce methane. Volumetric power consumption is in a range of from 0.03 Wh/L R to 0.3 Wh/L R . Current density is 0.01 A/cm E 2 or lower. The voltage is sufficient to electrolyze water without destroying microbial growth. Such a method results in improved electrolysis efficiency while avoiding the use of noble metal catalysts. Further, a combination of water electrolysis with anaerobic degradation of organic matter results in increased biogas quality and in increased biogas quantity and yield. Oxidation of hydrogen sulfide contributes to the increased quality, while an increase in the rate of organic matter hydrolysis and an increase in the production of methane from hydrogen contributes to the increased quantity and yield.

System for producing l-homophenylalanine and a process for producing l-homophenylalanine

The present invention relates to a system ( 10 ) for producing L-homophenylalanine and a process for producing L-homophenylalanine using the system ( 10 ). The system ( 10 ) and the process include monitoring and controlling of the reaction conditions (e.g., temperature and pH) to desired or predetermined values. The monitoring, adjusting and agitating steps provided by the method thereby result in a more complete conversion of the available substrate and produce a sufficient yield of homophenylalanine.

Recovery of higher alcohols from dilute aqueous solutions

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

Method and system for the gas-tight process control of percolators in a biogas method having two or more stages

The invention relates to a method and a system for obtaining biogas in two or more stages in a hydrolysis and a methane stage, wherein the hydrolysis of solid biogenic materials is performed in at least two percolators operated at offset times. Liquid hydrolyzate and COrich hydrolysis gas, and then hydrolysis gas comprising methane thereby arises in the percolator. The liquid hydrolyzate is removed from the percolators, wherein part of the hydrolyzate is fed into the methane stage and the other part of the hydrolysis stage. In the methane stage, the hydrolyzate is converted to biogas and fermenting fluid. In the method according to the invention, the percolators are operated in a gas tight manner and hydrolysis gas is drawn off from the percolators, wherein the hydrolysis gas comprising methane is fed to an energy utilization and COrich hydrolysis gas is used for purging a further percolator operated at an offset time. The system according to the invention is suitable for performing said method and comprises at least two gas tight percolators that are interconnected by means of the gas supply lines thereof, and at least one methane reactor. 1. A method for obtaining biogas in two or more stages by hydrolysis of solid biogenic material in at least two percolators operated at offset times , comprising a hydrolysis stage and a methane stage , the method comprising:{'sub': '2', 'hydrolyzing biogenic material in the hydrolysis stage to form in the percolator liquid hydrolysate and hydrolysis gas at first CO-rich hydrolysis gas being formed and afterwards methane-containing hydrolysis gas being formed,'}removing the hydrolysate from the percolators and collecting the hydrolysate,feeding one part of the hydrolysate into the methane stage and feeding the other part of the hydrolysate into the hydrolysis stage,converting the hydrolysate in the methane stage into biogas and fermentation liquid by methane-forming microorganisms,removing the fermentation liquid from the methane ...

Methods and Systems for the Production of Hydrocarbon Products

Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.

CELLULOSE SACCHARIFICATION APPARATUS, BIOMASS SACCHARIFICATION APPARATUS, FERMENTATION APPARATUS AND CELLULOSE SACCHARIFICATION METHOD

A fermentation apparatus (A) of the present invention comprising: an enzymatic reactor () for degrading cellulose using a diastatic enzyme, and a first catalytic reactor () for degrading the degradation product produced by the enzymatic reactor () into glucose, using a solid acid catalyst (X). According to this fermentation apparatus (A), saccharification treatment of cellulose can be performed while reducing diastatic enzyme costs. 1. A cellulose saccharification apparatus , comprising:an enzymatic reactor for degrading cellulose using a diastatic enzyme, anda first catalytic reactor for degrading the degradation product produced by the enzymatic reactor into glucose, using a solid acid catalyst.2. The cellulose saccharification apparatus according to claim 1 , wherein the diastatic enzyme is a heat-resistant enzyme.3. A biomass saccharification apparatus claim 1 , comprising:a pressurized hot water reactor for selectively degrading hemicellulose contained in biomass by allowing pressurized hot water to act on the biomass,a solid-liquid separator for separating cellulose as a solid from a treated liquid of the pressurized hot water reactor, and{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a cellulose saccharification apparatus of for degrading cellulose separated by the solid-liquid separator into glucose.'}4. The biomass saccharification apparatus according to claim 3 , further comprising a second catalytic reactor for degrading a hemicellulose degradation product as the liquid separated by the solid-liquid separator claim 3 , into a hemicellulose-derived monosaccharide claim 3 , using a solid acid catalyst.5. A fermentation apparatus claim 3 , comprising:{'claim-ref': {'@idref': 'CLM-00004', 'claim 4'}, 'the biomass saccharification apparatus of ,'}a first fermenter for producing fermentative products from glucose produced by the biomass saccharification apparatus, anda second fermenter for producing fermentative products from a hemicellulose-derived ...

APPARATUS FOR PRODUCING SACCHARIDE SOLUTION THAT USES BIOMASS AS FEEDSTOCK

There is provided an apparatus for treating a biomass feedstock at a high temperature, including a cooling means for cooling a biomass treated liquid at a high temperature; an enzymatic saccharification tank for saccharifying a cooled treated liquid with an enzyme; a solid-liquid separation apparatus for removing water-slightly soluble substances contained in a saccharide solution taken out from the enzymatic saccharification tank and a foreign substance removing unit provided with a microfiltlation (MF) membrane; a dilution tank, disposed downstream of the foreign substance removing unit, for diluting the saccharide solution from which the water-slightly soluble substances are removed by adding water thereto; a water separation unit, provided with a reverse osmosis (RO) membrane, for removing water from the diluted saccharide solution so as to obtain concentrated saccharide solution. 1. An apparatus for producing saccharide solution that uses biomass as a feedstock comprising:a hydrothermal decomposition apparatus for performing a high temperature and high pressure treatment in a temperature range of 180° C. to 240° C., while bringing a biomass feedstock including at least cellulose, hemicellulose, and lignin into countercurrent contact with pressurized hot water;a cooling means for cooling biomass treated liquid at a high temperature which has been discharged from the hydrothermal decomposition apparatus;an enzymatic saccharification tank for saccharifying the cooled treated liquid with an enzyme:a solid-liquid separation apparatus for removing a water-slightly soluble substance contained in the saccharide solution taken from the enzymatic saccharification;a foreign substance removing unit provided with a microfiltlation (MF) membrane;a dilution tank disposed downstream of the foreign substance removing unit, for adding water thereto so as to dilute the saccharide solution from which the water-slightly soluble substance has been removed; anda water separation unit ...

ENERGY PRODUCTION SYSTEMS UTILIZING RUMINANT ANIMAL METHANE EMISSIONS

A process for the utilization of the methane produced by enteric fermentation, specifically to a process that utilizes methane produced by ruminant animals through enteric fermentation as a source of carbon and/or energy for the directed production of methane-based goods or processes is provided. 19.-. (canceled)10. A system configured to simultaneously reduce environmental enteric fermentation methane emissions and produce energy , comprising:(a) one or more ruminant animals; wherein said first gas comprises enteric fermentation-derived methane gas that has been emitted by and collected from said animals,', 'wherein said second gas comprises oxygen and said first gas;, '(b) a first and second gas,'}(c) an energy-generating device that oxidizes methane to produce energy; and(d) a conveyor configured to direct, move, and/or convey said second gas to contact said energy-generating device;wherein introduction of said first gas and said second gas to said device results in oxidation of a portion of said first gas to methanol, water, and/or carbon dioxide, thereby simultaneously reducing environmental enteric fermentation methane emissions and producing energy.11. The system of claim 10 , wherein said second gas further comprises ammonia claim 10 , wherein said energy generated is heat claim 10 , and wherein said energy-generating device comprises an engine.12. The system of claim 10 , wherein said energy-generating device is selected from the group consisting of a fuel cell claim 10 , an engine claim 10 , a culture of microorganisms claim 10 , a reverse-flow reactor claim 10 , a turbine claim 10 , and/or a microturbine.13. The system of claim 10 , wherein said second gas further comprises one or more of air claim 10 , dust claim 10 , nitrogen claim 10 , gases claim 10 , carbon dioxide claim 10 , particulate matter claim 10 , and/or other airborne matter.14. The system of claim 13 , wherein one or more of dust claim 13 , nitrogen claim 13 , gases claim 13 , carbon ...

INTEGRATED SYSTEMS AND METHODS FOR ORGANIC ACID PRODUCTION

Crude bio-based organic acid-producing feedstock is used to produce a bio-based organic acid. Related systems and methods are also described such as an integrated method including a step of producing a crude polyol product and then processing the crude polyol product to produce a bio-based organic acid. 1. An integrated method for producing a bio-based organic acid comprising:processing, in a first apparatus, a carbon source-producing feedstock to produce a crude polyol product; andprocessing, in a second apparatus, the crude polyol product to produce a bio-based organic acid, wherein the second apparatus is a fermentor and has an inlet in fluid communication with an outlet of the first apparatus.2. The method of claim 1 , wherein the carbon source-producing feedstock comprises hydrolyzed biomass.3. The method of claim 1 , wherein the carbon source-producing feedstock is selected from the group consisting of monomeric sugars claim 1 , oligomeric sugars claim 1 , and mixtures thereof.4. The method of claim 1 , wherein the carbon source-producing feedstock comprises fructose claim 1 , glucose claim 1 , or mixtures thereof.5. The method of claim 1 , wherein the crude polyol product comprises a sugar alcohol.6. The method of claim 1 , wherein the crude polyol product comprises a sugar alcohol selected from the group consisting of methanol claim 1 , glycol claim 1 , glycerol claim 1 , erythritol claim 1 , threitol claim 1 , arabitol claim 1 , xylitol claim 1 , ribitol claim 1 , mannitol claim 1 , sorbitol claim 1 , galactiol claim 1 , iditol claim 1 , inositol claim 1 , volemitol claim 1 , isomalt claim 1 , malitol claim 1 , lactitol claim 1 , polyglycitol claim 1 , and combinations thereof.7. The method of claim 1 , wherein the crude polyol product comprises sorbitol.8. The method of claim 1 , wherein the bio-based organic acid comprises a dicarboxylic acid.9. The method of claim 1 , wherein the bio-based organic acid is selected from the group consisting of pyruvic ...

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. An automated stand-alone multi-module cell editing instrument for performing nuclease-directed editing in mammalian cells comprising:a housing configured to house all or some of the modules;a receptacle configured to receive the mammalian cells;one or more receptacles configured to receive expression vectors, wherein the expression vectors comprise a viral vector backbone and an editing cassette;a growth module for growing cells for transfection;a transfection module configured to introduce the expression vectors into the mammalian cells;a nuclease-directed editing module configured to allow the introduced expression vectors to edit nucleic acids in the mammalian cells;a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; andan automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the transformation module and from the transformation module to the nuclease-directed editing module without user intervention.2. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the automated liquid handling system comprises a sipper or pipettor.3. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the nuclease-directed editing module is also a recovery module.4. The automated stand-alone multi-module cell editing instrument of claim 1 , further comprising a recovery module separate from the editing module.5. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the growth module is separate from the transfection module.6. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the growth module and the transfection module are a ...

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. An automated multi-module cell editing instrument comprising:a housing configured to house all of some of the modules;a receptacle configured to receive cells;one or more receptacles configured to receive nucleic acids;a transformation module configured to introduce the nucleic acids into the cells;a recovery module configured to allow the cells to recover after cell transformation in the transformation module;an editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells; anda processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script.2. The automated multi-module cell editing instrument of claim 1 , wherein the nucleic acids in the one or more receptacles comprise a backbone and an editing cassette claim 1 , and the automated multi-module cell editing instrument further comprises a nucleic acid assembly module.3. The automated multi-module cell editing instrument of claim 2 , wherein the nucleic acid assembly module comprises a magnet.4. The automated multi-module cell editing instrument of claim 3 , wherein the nucleic acid assembly module is configured to perform isothermal nucleic acid assembly.5. The automated multi-module cell editing instrument of claim 1 , wherein the editing module and the recovery module are combined.6. The automated multi-module cell editing instrument of claim 1 , further comprising a growth module configured to grow the cells.7. The automated multi-module cell editing instrument of claim 6 , wherein the growth module measures optical density of the growing cells.8. The automated multi-module cell editing instrument of claim 7 , wherein optical density is measured continuously.9. The automated multi-module cell editing instrument of claim ...

MICROALGAE-BASED SOIL INOCULATING SYSTEM AND METHODS OF USE

Some embodiments include a microalgae culturing system including a bioreactor adapted to propagate microalgae in a culture solution using in combination at least one of natural and artificial light, and at least one nutrient including at least a carbon source, where the microalgae are freely suspended in and form part of the culture solution. A microalgae feed source is coupled to the bioreactor and a first controller between a water conditioning assembly and the bioreactor. The water conditioning assembly is coupled as an input of supply water to the bioreactor, and configured to condition the supply water to a specified purity that enables substantially unhindered growth of the microalgae in the culture solution to a specified concentration, and the first controller is configured to control supply of the microalgae feed source to the bioreactor. 1. A culturing system comprising:a bioreactor adapted to propagate microalgae in a culture solution using in combination at least one of natural and artificial light, and at least one nutrient comprising at least a carbon source, wherein the microalgae are freely suspended in and form part of the culture solution; anda water conditioning assembly;an algae nutrient supply coupled to the bioreactor; a first controller configured for controlling fluid flow between the water conditioning assembly and the bioreactor, the water conditioning assembly coupled as an input of supply water to the bioreactor, and configured to condition the supply water to a specified purity that enables substantially unhindered growth of the microalgae in the culture solution to a specified microalgae concentration, and wherein the first controller is configured to control delivery of the algae nutrient supply to the bioreactor; and a carbon dioxide source coupled to the bioreactor, wherein the carbon dioxide is injected into the culture solution as the carbon source.2. The system of claim 1 , further comprising a second controller coupled to a probe ...

IMPROVED DEVICE FOR REMOVING VOLATILE ORGANIC COMPOUNDS

A device for the removal of volatile organic compounds including at least one first connector arranged at the outlet of a gas stream to be treated. At its top, has a connection extension that links with the bioreactor located in the middle part of the device (having a nutrient solution where organisms that degrade volatile organic compounds can grow). Additionally, there is a dispersion arrangement that couples with an outlet through which the gaseous stream, once treated, is expelled out to the environment. A distribution network of a nutritive solution with microorganisms that degrade volatile organic compounds. The connection extension includes a distributor cone that is arranged in an inverted way and internally emptied and also has a cylindrical extension at its top. The distributor cone allows the airflow to be displaced by an external path into the internal walls of the distributor cone, whose cylindrical extension connects with a second perforated cylinder with multiples drill holes throughout its periphery. The axial axis of the cylindrical extension coincides with the axial axis of the distributor cone. The perforations of this second perforated cylinder are arranged so that the gas output can enter the bioreactor from its bottom. 1. A device for the removal of volatile organic compounds comprising:{'b': '100', 'at least one first connector () arranged at an outlet of a gas stream to be treated;'}{'b': '200', 'a connection extension () located at a top end of the device;'}{'b': '300', 'a bioreactor () connected to the connection extension, the bioreactor is located in a middle part of the device, the bioreactor contains a nutrient solution where organisms that degrade volatile organic compounds grow);'}{'b': '400', 'a dispersion arrangement () located a top end of the bioreactor;'}{'b': '500', 'an outlet () coupled to the dispersion arrangement, in the outlet the gaseous stream, once treated, is expelled out to the environment;'}{'b': '600', 'a ...

Global recycling of the earth's natural resources

The present disclosure deals with is a combination of interconnected innovative technologies for exploiting the advantages of closed cyclic chain reactions on a global scale to solve the contemporary problem of carbon emissions and the earth's diminishing raw resources associated with fossil fuelled power generation and widespread dumping practices. As a first step the surest way to challenge the present monopoly of the fossil fuel industry is to exploit the full potential of photosynthesising processes, whereby carbon remains the key chemical element for fulfilling the needs of contemporary and future civilisations and whereby the means will be available to control the concentration of green house gases at optimal levels in the environment. Envisaged are global recycling trading schemes based on captive carbon processes and systems for the conversion of existing fossil fuelled power stations to cyclic global power generation using photosynthesised fuel, whereby emitted carbon gases are recycled to sites of photosynthesis for further fuel production thereby creating closed cyclic trading systems with the possibility of completely eliminating global greenhouse gas emissions into the environment from power generation. The further innovations of the present disclosure consist of alternatives to fossilised carbon for transport based on global cyclic photosynthesising systems as well as the production of potable water and a host of carbon based commodities such as furnishings, textiles, pharmaceuticals, chemicals and food production to achieve maximum mitigation of environmental pollution combined with economically viable carbon-based processes.

CULTURE SYSTEMS AND METHODS OF USING SAME

Culture systems and methods of using same. The systems include a housing defining an inner space. The inner space includes a headspace and at least a portion of a reservoir. A surface for immobilizing cells is moveable between the headspace and the reservoir. The systems can be used for coculturing methanotrophs and phototrophs for processing biogas and wastewater, particularly from anaerobic digesters. 1. A system comprising:a housing comprising a top and sides and defining an inner space extending between the sides and to the top;a headspace in an upper portion of the inner space;a reservoir comprising at least a first reservoir portion, wherein the first reservoir portion is in a lower portion of the inner space, the headspace and the reservoir are defined with respect to each other within the inner space by a horizontal plane spanning the inner space, and the headspace and the reservoir do not overlap within the inner space; anda surface comprising a surface portion capable of being cycled between the headspace and the reservoir.2. The system of claim 1 , wherein the housing separates the headspace from a surrounding space claim 1 , wherein the housing is capable of maintaining a gaseous composition of the headspace that is different from a gaseous composition of the surrounding space.3. The system of claim 1 , wherein the housing forms an enclosure enclosing the inner space claim 1 , wherein the reservoir is entirely encompassed within the inner space.4. The system of wherein the reservoir further includes a second reservoir portion contiguous with the first reservoir portion claim 1 , the second reservoir portion does not overlap with the first reservoir portion and does not overlap with the inner space claim 1 , the housing separates the headspace from a surrounding space claim 1 , and the second reservoir portion is contiguous with the surrounding space.5. The system of claim 1 , wherein the headspace is filled with a gas claim 1 , the gas comprises at least ...

METHOD AND SYSTEM FOR CONVERTING ELECTRICITY INTO ALTERNATIVE ENERGY RESOURCES

A method of using electricity to produce methane includes maintaining a culture comprising living methanogenic microorganisms at a temperature above 50° C. in a reactor having a first chamber and a second chamber separated by a proton permeable barrier, the first chamber comprising a passage between an inlet and an outlet containing at least a porous electrically conductive cathode, the culture, and water, and the second chamber comprising at least an anode. The method also includes coupling electricity to the anode and the cathode, supplying carbon dioxide to the culture in the first chamber, and collecting methane from the culture at the outlet of the first chamber. 118-. (canceled)19. A method for producing methane , the method comprising:{'b': 218', '216', '202', '204', '206', '212', '216', '214', '218', '220', '218', '216', '230', '232', '218', '216, '(a) supplying electricity to an anode () and a porous electrically conductive cathode () of a biological reactor (), wherein the biological reactor (i) is coupled to a source of electricity () and a source of carbon dioxide (), (ii) has at least a first chamber () containing at least the cathode (), a culture comprising methanogenic microorganisms, and water, a second chamber () containing at least the anode (), and a proton permeable, gas impermeable barrier () separating the anode () from the cathode (), and (iii) has a current collector (, ) coupled to each of the anode () and the cathode (),'}{'b': 216', '238', '220', '230', '216', '234', '236, 'wherein the cathode () is impregnated with the methanogenic microorganisms and with an aqueous electrolytic medium, wherein the methanogenic microorganisms are in a passage () formed between the barrier () and the current collector () coupled to the cathode () and located between an inlet for carbon dioxide () and an outlet for methane (),'}{'b': 238', '212, 'wherein the culture is maintained in the aqueous electrolytic medium in the passage () of the first chamber () ...

METHOD AND SYSTEM FOR PROVIDING BUFFER SOLUTIONS

A method for delivering a processing solution includes providing a concentrated processing solution in a bioprocess container, the concentrated processing solution being produced at a first site. The method also includes combining the concentrated processing solution with a biopolymer containing solution produced in a bioreactor at a second site different from the first site. In some embodiments, the processing solution is a buffer for processing products of cells cultured in a bioreactor. A system and a pharmaceutical production facility for carrying out the method is also provided. 1. A system for delivering a processing solution , the system comprising:a bioprocess container containing a concentrated processing solution, the concentrated processing solution produced at a first site;a processing area having at least one processing unit at a second site different from the first site; anda pipe connecting the bioprocess container to the at least one processing unit, the pipe having a valve to allow the concentrated processing solution to flow from the bioprocess container to the at least one processing unit to combine the concentrated processing solution with a biopolymer containing solution produced in the at least one processing unit,wherein the processing solution is at least one of a buffer and media.2. The system of claim 1 , further comprising a controller connected to the at least one processing unit claim 1 ,wherein the concentrated processing solution is provided based on a determination by the controller that the processing solution is required when the determination is communicated from the second site to the first site.3. The system of claim 1 , wherein the system is configured to dilute the concentrated processing solution prior to combining with the biopolymer containing solution.4. The system of claim 1 , wherein the processing solution is one of Tris claim 1 , Tris-Base claim 1 , Tricine claim 1 , HEPES claim 1 , MOPS claim 1 , PIPES claim 1 , TAPS ...

Perfusion bioreactor and related methods of use

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

METHOD AND APPARATUS FOR PRODUCING ORGANIC SUBSTANCES

It is an object of the present invention to convert COto CO by bringing about a reverse shift reaction in a relatively low-temperature condition, and thereby enhancing efficiency of generating organic substances, such as ethanol. A raw material gas g containing COand His subjected to a reverse shift reaction in a reverse shift reactor Organic substances are generated from a raw material gas g after the reverse shift reaction in an organic substance generator In the subjecting step, the raw material gas g is brought into contact with a reverse shift reaction catalyst The reverse shift reaction catalyst includes a support and a catalyst metal supported by the support The catalyst metal includes a transition metal. The catalyst metal is preferably composed of Fe with at least one kind of metal from among Al, Ga, In, Cu, Ag, Au, Pd and Mn added thereto. 1. A method for producing organic substances from a raw material gas containing COand H , the method comprising steps of:subjecting the raw material gas to a reverse shift reaction; andgenerating organic substances from the raw material gas after the reverse shift reaction, wherein:the raw material gas is contacted with a reverse shift reaction catalyst in the subjecting step;the reverse shift reaction catalyst includes a support and a catalyst metal supported by the support; andthe catalyst metal includes a transition metal.2. The method according to claim 1 , wherein the catalyst metal includes Fe with at least one kind of metal selected from a group of Al claim 1 , Ga claim 1 , In claim 1 , Cu claim 1 , Ag claim 1 , Au claim 1 , Pd claim 1 , and Mn added thereto.3. The method according to claim 1 , wherein the catalyst metal includes Fe with Pd added thereto.4. The method according to claim 1 , wherein a temperature condition in the subjecting step is from 150 degrees C. to 500 degrees C.5. The method according to claim 1 , wherein an offgas generated in the generating step is mixed with the raw material gas before ...

Systems and methods for converting biomass to biocrude via hydrothermal liquefaction

Systems and processes of providing novel thermal energy sources for hydrothermal liquefaction (HTL) reactors are described herein. According to various implementations, the systems and processes use concentrated solar thermal energy from a focused high-energy beam to provide sufficient energy for driving the HTL biomass-to-biocrude process. In addition, other implementations convert biowaste, such as municipal biosolids and grease and food waste, to biocrude using anaerobic digesters, and a portion of the biogas generated by the digesters is used to produce the thermal and/or electrical energy used in the HTL reactor for the biomass-to-biocrude process. Furthermore, alternative implementations may include a hybrid system that uses biogas and solar radiation to provide sufficient thermal energy for the HTL reactor.

SILAGE WASTE RECOVERY PROCESS, FACILITY, SYSTEM, AND PRODUCT

A silo of conventional construction is provided with a collection pan at its bottom to collect effluent. The effluent can be stored and/or processed. Processing can include separation of water to concentrate the effluent. The effluent can contain fermentation product, such as alcohol, generated within the silo prior to collection, and it can also be fermented after collection. Processed effluent can be handled safely without risk of contamination to ground water at the silo site. 1. A facility for obtaining a fermentation product from silage , said facility comprising:a) at least one silage silo having a bottom;b) a collector at said bottom of said silo for collecting a silage juice, said collector including a primary drain from said silo and a drain line;c) a storage tank for storing silage juice collected by said collector, said storage tank connected to said primary drain of said collector via said drain line; a secondary drain, situated at a higher position than the primary drain on said silo, for indicating seepage backup and accumulation within said silo; and', 'an overflow tank, connected to and separate from said storage tank, and operative to collect overflow seepage from said storage tank., 'd) an overflow assembly for controlling an overflow of silage juice draining from said silo, said overflow assembly operative to prevent a backup of silage juice within said silo, and comprising'}2. The facility of claim 1 , wherein said storage tank is located underground.3. The facility of claim 1 , wherein said drain line includes at least one filter assembly for filtering said silage juice prior to its storage in said storage tank claim 1 , said at least one filter assembly including a reverse osmosis separation device operative to separate usable water from said silage juice claim 1 , said reverse osmosis separation device feeding concentrated silage juice to said storage tank over said drain line.4. The facility of claim 1 , wherein said storage tank is provided ...

ELECTROPORATION, DEVELOPMENTALLY-ACTIVATED CELLS, PLURIPOTENT-LIKE CELLS, CELL REPROGRAMMING AND REGENERATIVE MEDICINE

The claimed invention is directed towards a novel combination cell electroporation/cell culturing apparatus which can be termed a cell culture dish suitable for in vitro electroporation, and towards a device suitable for in vivo electroporation—both useful in methods suitable for the generation of developmentally-activated, pluripotent, pluripotent-like, multipotent, and/or self-renewing cells which are capable of beginning to differentiate in culture into a variety of cell types and capable of further differentiation in vivo. The claimed invention is also directed towards the generation of desirable, differentiating somatic cell populations transplantable to animals or patients, genetic modification of endogenous and exogenous cells, and the treatment of patients suffering from diseases that may be ameliorated by these methods. This invention also provides methods for preventing, treating, or retarding disease, for example, immunodeficiency virus (e.g. HIV-1, HIV-2, SIV, FIV, etc.) infection. 1. A cell or tissue culture system comprising one or more first compartment with dimensions for electroporation , the one or more first compartment permitting communication with a second compartment that permits incubation and/or comprises an inspection or visualization plane for microscopic observation , and wherein the cell or tissue culture system is configured to permit one or more cells electroporated in the one or more first compartment in a first medium to be incubated and cultured in the second compartment in a second medium , thereby eliminating infectious and contamination risks associated with transfer of cells between a cell or tissue culture system/and a distinct electroporation apparatus , and wherein , the one or more first compartment isa. external the second compartmentb. internal to the second compartmentc. located inferiorly to the second compartment,d. located laterally to the second compartment,d. oriented perpendicularly to the second compartment,e. ...

METHOD AND STRUCTURE FOR COMPREHENSIVE UTILIZATION OF CO-PRODUCTS OF ALCOHOL PRODUCTION FROM GRAINS

A method is provided for treating distiller's grains with solubles (DGS) to produce one or more byproducts. The method includes separating the DGS into a low protein mixture and a high protein mixture. The method includes generating, from the low protein mixture, a biogas by an anaerobic digestion process. The method includes generating, from the high protein mixture, at least one of a vegetable oil from a vegetable oil separation process, a high protein animal feed from a separation process and a microalgae biomass material from a microalgae production process. 1. A method for treating distiller's grains with solubles (DGS) to produce one or more byproducts , the method comprising:separating the DGS into a low protein mixture and a high protein mixture;generating biogas, from the low protein mixture, by an anaerobic digestion process; and a vegetable oil from a vegetable oil separation process;', 'a high protein animal feed from a separation process; or', 'a microalgae biomass material from a microalgae production process., 'generating, from the high protein mixture, at least one of2. The method of claim 1 , wherein the separating the DGS comprises at least one of centrifuging claim 1 , filtration claim 1 , or pressing.3. The method of claim 1 , wherein a protein content in the DGS is less than or equal to about 30%.4. The method of claim 1 , wherein a protein content in the DGS is less than or equal to about 20%.5. The method of claim 1 , wherein the steps of separating the DGS comprise at least one of anaerobic digestion and drying for combustion.6. The method of claim 1 , wherein if the DGS has a moisture content that is less than or equal to 80% claim 1 , adding liquid to the DGS to raise the moisture content to 80% or greater.7. The method of claim 1 , wherein the step of separating the DGS comprises steeping claim 1 , size reduction claim 1 , and then separating of liquid from solid.8. The method of claim 1 , wherein the step of separating the DGS comprises ...

CYCLONE SEPARATOR ARRANGEMENT AND METHOD

A cyclone separator () comprises a pressure chamber (), an inlet () for an incoming flow of a mixture of gas and particles, a gas outlet () for outgoing gas arranged through a top wall () of the pressure chamber and a particle outlet () for outgoing particles arranged in a lower part () of the pressure chamber. The pressure chamber has a main rotation symmetric shape. The inlet is arranged through a side wall () of an upper part () of the pressure chamber for directing the incoming flow with a main velocity component in a tangential direction. The inlet comprises an inlet tube () protruding through the side wall of the upper part into the pressure chamber, whereby an inner end () of the inlet tube is provided at a position interior of the pressure chamber. A method for operating a cyclone separator is also disclosed. 1. A cyclone separator , comprising:a pressure chamber having main rotation symmetric shape;an inlet for an incoming flow of a mixture of gas and particles, said inlet being arranged for directing said incoming flow with a main velocity component in a tangential direction with respect to said rotation symmetric shape;a gas outlet for outgoing gas arranged through a top wall of said pressure chamber; anda particle outlet for outgoing particles arranged in a lower part of said pressure chamber;wherein said inlet being arranged through a side wall of an upper part of said pressure chamber and comprises an inlet tube protruding through, in said tangential direction, said side wall of said upper part of said pressure chamber into said pressure chamber, whereby an inner end of said inlet tube is provided at a position interior of said pressure chamber.2. The cyclone separator according to claim 1 , wherein an absolute measure of an angle between a line between a center of said pressure chamber and a radially inner edge of said inner end of said inlet tube and a center line claim 1 , said center line being a line that is perpendicular to said tangential ...

BLOOD CIRCULATION FOR CULTURE GROWTH

Blood being circulated to mimic the environment it naturally inhabits increases growth of cultures including bacteria for analysis. The present disclosure presents examples of a device, method, computer-readable medium, and other techniques for simulating a natural environment for blood. The techniques may include a channel with a hydrophobic interior surface and a fluid mover to encourage continuous fluid flow through the channel. The techniques may further include a gas exchange opening of the channel that exposes a fluid to gas outside of the channel. 1. A device for blood circulation as an environment for culturing target microorganisms comprising:a channel with a hydrophobic interior surface;a fluid mover to encourage continuous fluid flow through the channel; anda gas exchange opening of the channel that exposes a fluid to gas outside of the channel.2. The device of claim 1 , wherein the gas exchange opening is located on a loop shaped to steer fluid flow away from the channel and then back into the channel.3. The device of claim 1 , wherein the gas exchange opening is smaller than six micrometers.41. The device of claim claim 1 , wherein the fluid mover is a thermal inkjet resistor.5. The device of claim 4 , wherein the gas exchange opening is space between the thermal inkjet resistor and a reservoir in a path of the channel.6. The device of claim 1 , comprising a glucose storage compartment attached to the channel allowing exchange of glucose with the channel.7. The device of claim 1 , wherein the channel comprises a metabolite detector embedded into a die surface.8. The device of claim 1 , comprising a particle detector in the gas exchange opening to report a gas scatter profile at a plurality of times.9. The device of claim 1 , comprising a toxin removing balancer to selectively remove identified toxins from the fluid and balance the fluid to contain identified metrics.10. The device of claim 1 , wherein the gas exchange opening is covered by a gas- ...

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. An automated stand-alone multi-module cell editing instrument comprising:a housing configured to house all or some of the modules;a receptacle configured to receive mammalian cells;one or more receptacles configured to receive expression vectors, wherein the expression vectors introduce a desired DNA change to the target region and remove a proto-spacer motif (PAM) region from the target region;a growth module for growing the mammalian cells for transformation;a transformation module configured to introduce the expression vectors into the mammalian cells;a nuclease-directed editing module configured to allow the introduced expression vectors to edit nucleic acids in the mammalian cells;a magnetic separation module configured to separate edited mammalian cells from unedited mammalian cells;a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; andan automated liquid handling system to move liquids from the cell receptacle to the growth module; from the growth module to the transformation module; from the one or more receptacles configured to receive expression vectors to the transformation module; from the transformation module to the nuclease-directed editing module; and from the nuclease-directed editing module to the magnetic separation module without user intervention.2. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the automated liquid handling system comprises a sipper or pipettor.3. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the nuclease-directed editing module is also a recovery module.4. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the automated stand-alone multi-module cell ...

Automated cell processing methods, modules, instruments, and systems

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

AUTOLOGOUS CELL MULTIDIMENSIONAL MOLDING APPARATUS

The present invention discloses an autologous cell multidimensional molding apparatus, which can customize an artificial implant by using an autologous cell. The autologous cell multidimensional molding apparatus comprises a barotropic room, an autologous cell culturing module, a supplying module, a multi-axis molding module, and an implant saving module. The autologous cell culturing module cultivates the autologous cells and sends the autologous cells to the supplying module to mix with polymer materials to be combined autologous cells. The multi-axis molding module prints the combined autologous cells to be artificial implants for carrying and saving the combined autologous cells by the implant saving module. Wherein, the barotropic room is filled with a gas having pressure greater than an ambient pressure. The modules listed above are all inside the barotropic room and connected by closed pipes. Thus, compared to the prior art, the present invention can customize the artificial implants and effectively enhance the yield of the preparation of the artificial implants. 1. An autologous cell multidimensional molding apparatus , for customizing an artificial implant by using an autologous cell , comprising:a barotropic room, for inputting a gas having a pressure greater than an ambient pressure;an autologous cell culturing module, configured in the barotropic room, comprising a culture vessel and a feed pipe, wherein the culture vessel is used for culturing the autologous cell into a cultured autologous cell, and the feed pipe is connected to the culture vessel for receiving the cultured autologous cell;a supplying module, configured in the barotropic room, comprising a peristaltic feeder and a discharge pipe, wherein the peristaltic feeder is connected to the feed pipe for receiving the cultured autologous cell and mixing the cultured autologous cell with a polymer material to be a combined autologous cell, and the discharge pipe is connected to the peristaltic ...

SYSTEMS, DEVICES AND METHODS FOR AGRICULTURAL PRODUCT PULPING

This disclosure relates to systems, devices and methods for pulping agricultural products. Features for ensiling agricultural crops, separating solids and liquids, and processing the solids and liquids for use in a variety of products and processes are disclosed. For instance, systems and methods are disclosed for pulping grass crops, making products for energy conversion processes, screening fine debris, cell bursting, using strongly alkaline chemicals in pulping processes, and using sorghum plant for producing pulp. 1. An ensilage system for pulping agricultural crop , the system comprising:an ensilage facility configured to store the crop;a separation stage coupled with the ensilage facility, the separation stage configured to receive the crop from the ensilage facility and to separate solids from liquids in the crop;a liquids pathway coupled with the separation stage and configured to receive the liquids separated in the separation stage and to process the liquids for energy production; anda solids pathway coupled with the separation stage and configured to receive the solids separated in the separation stage and to process the solids into pulping product.2. The system of claim 1 , wherein the ensilage facility is configured for storing the crop long term for pulping production.3. The system of claim 1 , wherein the ensilage facility is configured for storing wet crop.4. The system of claim 1 , wherein the ensilage facility is further configured to compact the crop.5. The system of claim 1 , wherein the ensilage facility is further configured to enable long term storage without significant biological decay of organic compounds in the crop to exposure to oxygen while increasing sugar availability for purpose of energy production.6. The system of claim 1 , wherein the ensilage facility is further configured to enable long term storage of crop without exposure to molds and fungus.7. The system of claim 1 , wherein the crop is grass crop.8. The system of claim 1 , ...

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. A method for simultaneously editing up to 10 ,000 ,000 genomic sites in a population of live cells in an automated stand-alone multi-module cell editing instrument , comprising the steps of:providing a population of live cells in a receptacle configured to receive the live cells;providing first nucleic acids comprising up to 10,000,000 editing cassettes to one or more receptacles configured to receive first nucleic acids;growing the live cells in a growth module to a desired optical density to produce grown live cells;filtering and rendering electrocompetent the grown live cells in a filtration module to produce electrocompetent live cells;transforming the electrocompetent live cells in a transformation module configured to introduce the first nucleic acids into the electrocompetent live cells to produce transformed cells;providing conditions in a nuclease-directed editing module to allow the first nucleic acids to edit nucleic acids in the transformed cells to produce edited cells; andusing an automated liquid handling system to 1) transfer the first nucleic acids from the one or more receptacles configured to receive nucleic acids to the transformation module, 2) transfer the live cells from the receptacle configured to receive the live cells to the growth module, 3) transfer the grown live cells from the growth module to the filtration module, 4) transfer the electrocompetent cells from the filtration module to the transformation module, and 5) transfer the transformed cells from the transformation module to the nuclease-directed editing module without user intervention.2. The method of editing live cells according to claim 1 , wherein the cells are prokaryotic cells.3. The method of editing live cells according to claim 2 , wherein the cells are gram positive bacterial cells.4. The method ...

HYBRID LINEAR ACTUATOR CONTROLLED HYDRAULIC CELL STRETCHING

A hydraulic cell stretching device comprising a source of variable pressured hydraulic fluid hydraulically coupled to a flexing chamber. The flexing chamber has at least one cell well. The cell well has a membrane subjected to the variable pressured hydraulic fluid. 1. A hydraulic cell stretching device comprising:a source of variable pressured hydraulic fluid hydraulicly coupled to a flexing chamber;the flexing chamber having at least one cell well; andthe cell well having a membrane subjected to the variable pressured hydraulic fluid.2. The device of wherein the source of source of variable pressured hydraulic fluid includes a linear actuator coupled to a piston and cylinder assembly.3. The device of wherein the linear actuator is an electro-mechanical actuator powered by a stepper motor.4. The device of wherein the stepper motor allows fine gradations of linear travel of an actuator rod per step such that the cell well membrane may repeatable flex at a maximum length of 15 microns.5. The device of further comprising a linear encoder.6. The device of wherein the linear encoder includes a sensor claim 5 , transducer or readhead paired with a scale that encodes position and provides feedback to a computer controller with regard to precise positioning of one of the actuator rod claim 5 , the length of piston strokes claim 5 , the speed of piston strokes and the number of piston strokes per minute.7. The device of wherein a plurality of cell wells are positioned in a single baseplate.8. The device of wherein a first column of the plurality of wells are fluidically connected to one another and experience a first hydraulic pressure from the pressure source.9. The device of wherein a second column of the plurality of cell wells are fluidically connected to one another but are fluidically separate from the cell wells of the first column.10. The device of wherein the second column of the plurality of wells simultaneously experience a second hydraulic pressure from the ...

SYSTEM AND METHOD FOR CARBON DIOXIDE REACTOR CONTROL

A system optionally including a carbon oxide reactor. A method for carbon oxide reactor control, optionally including selecting carbon oxide reactor aspects based on a desired output composition, running a carbon oxide reactor under controlled process conditions to produce a desired output composition, and/or altering the process conditions to alter the output composition.

DEVICE AND METHOD FOR THE SEQUESTRATION OF ATMOSPHERIC CARBON DIOXIDE

The invention relates to a device and to a method for sequestering atmospheric carbon dioxide using at least one air capture module in conjunction with a bioreactor equipped with an autotrophic microorganisms. 1. A device for sequestering atmospheric carbon dioxide , the device comprising at least one module comprising a capture unit binding atmospheric carbon dioxide by way of an adsorber material and , after treatment by way of heat or a vacuum , the atmospheric carbon dioxide being kept available , and the module being connected to at least one bioreactor , wherein the atmospheric carbon dioxide is continuously supplied to autotrophic microorganisms in at least one bioreactor.2. The device for sequestering atmospheric carbon dioxide according to claim 1 , wherein the atmospheric carbon dioxide is kept available in a container.3. A device for sequestering atmospheric carbon dioxide claim 1 , the device comprisinga module comprising a capture unit, wherein atmospheric carbon dioxide is bound by way of an adsorber material and, after treatment by way of heat or a vacuum, the atmospheric carbon dioxide is kept available in a pressurized container, andat least one bioreactor containing autotrophic microorganisms.4. A device for sequestering atmospheric carbon dioxide according to claim 1 , further comprising at least one gas-liquid separator.5. A device for sequestering atmospheric carbon dioxide according to claim 1 , wherein at least one bioreactor is a photobioreactor or an open pond bioreactor.6. A device for sequestering atmospheric carbon dioxide according to claim 1 , wherein at least one module is an air capture module.7Scenedesmus, Spirulina, Nannochloropsis, NostocChlorococcus.. A device for sequestering atmospheric carbon dioxide according to wherein the autotrophic microorganisms are photoautotrophic microorganisms or chemoautotrophic microorganisms claim 1 , in particular Archaea bacteria claim 1 , algae claim 1 , micro algae claim 1 , or8. A device for ...

Method and Apparatus for Reducing CO2 in a Stream by Conversion to a Syngas for Production of Energy

A system and method for producing Syngas from the COin a gaseous stream, such as an exhaust stream, from a power plant or industrial plant, like a cement kiln, is disclosed. A preferred embodiment includes providing the gaseous stream to pyrolysis reactor along with a carbon source such as coke. The COand carbon are heated to about 1330° C. and at about one atmosphere with reactants such as steam such that a reaction takes place that produces Syngas, carbon dioxide (CO) and hydrogen (H). The Syngas is then cleaned and provided to a Fischer-Tropsch synthesis reactor to produce Ethanol or Bio-catalytic synthesis reactor. 1. An apparatus for producing syngas that reduces an amount of carbon dioxide in a gaseous stream , the apparatus comprising:a reaction chamber, said reaction chamber selected to be one of a pyrolysis reactor, a conventional gasifier or a plasma arc gasifier;a source of carbonaceous material provided to said reaction chamber;{'sub': '2', 'a source of HO provided to said reaction chamber;'}{'sub': '2', 'a gaseous stream source containing carbon dioxide (CO) provided to said reaction chamber;'}{'sub': 2', '2', '2', '2', '2', '2, 'said reaction chamber having a temperature of between 400° C. and 5000° C. and a pressure of about one bar or greater to react materials in said reaction chamber, the reacting materials consisting essentially of said carbonaceous material, said HO and said carbon dioxide (CO) in said gaseous stream to form syngas comprising carbon monoxide (CO) and hydrogen (H), and carbon dioxide (CO), and wherein an amount of said COin said syngas is at least 53% less than an amount of carbon dioxide (CO) that was contained in said gaseous stream; and'}means for discharging said formed syngas.2. The apparatus of claim 1 , wherein said source of HO provided as a reactant into said reaction chamber is steam.3. The apparatus of claim 1 , wherein the reaction chamber is maintained at a temperature of between 400° C. and 2000° C.4. The apparatus ...

SOLID WASTE PROCESSING WITH PYROLYSIS OF CELLULOSIC WASTE

Waste, such as municipal solid waste (MSF), is separated into a wet fraction and refuse derived fuel (RDF). For example, the waste may be separated in a press. The wet fraction is treated in an anaerobic digester. The RDF is further separated into a cellulosic fraction and a non-cellulosic fraction. The cellulosic fraction is treated by pyrolysis and produces a pyrolysis liquid. The pyrolysis liquid is added to the anaerobic digester. 1. A process comprising steps of ,separating waste to produce rejects and a wet fraction;separating the rejects into cellulosic rejects and non-cellulosic rejects; and,pyrolysing the cellulosic rejects.2. The process of further comprising treating the wet fraction in an anaerobic digester.3. The process of wherein pyrolysing the cellulosic rejects produces a liquid pyrolysis product claim 1 , and further comprising treating the liquid pyrolysis product in an anaerobic digester.4. The process of comprising treating sludge from the anaerobic digester by pyrolysis.5. The process of wherein the pyrolysis is conducted at a temperature ranging from 270 to 450 degrees C.6. The process of wherein the pyrolysis is conducted at a residence time of 5 to 30 minutes.7. The process of wherein the step of separating waste comprises screening.8. The process of wherein the step of separating waste comprises pressing waste.9. The process of wherein the waste comprises municipal solid waste.10. The process of further comprising treating the wet fraction in the anaerobic digester.11. A process for treating solid waste comprising steps of claim 1 ,separating the waste to produce rejects and a wet fraction;separating cellulosic rejects from the rejects; and,treating at least a portion of cellulosic rejects by anaerobic digestion.12. The process of further comprising co-digesting the wet fraction with the at least a portion of cellulosic rejects.13. The process of wherein the step of separating the waste comprises pressing the waste;14. The process of ...

CONVERSION OF BIOMASS, ORGANIC WASTE AND CARBON DIOXIDE INTO SYNTHETIC HYDROCARBONS

A process and system for producing a synthetic hydrocarbon having a desired H/C ratio is disclosed. Organic material is biochemically digested in a two stage biodigester for separately producing a hydrogen containing biogas substantially free of methane in a first stage and a methane containing biogas in a second stage. The methane containing biogas is reformed in a first reformer to generate hydrogen gas and carbon monoxide gas, which are then combined in a mixer with the hydrogen containing biogas into a syngas in amounts to achieve in the syngas an overall H/C ratio substantially equal to the desired H/C ratio. The syngas is reacted with a catalyst in a second reformer, a Fischer-Tropsch (FT) reactor, to produce the hydrocarbon. Using a two stage biodigester allows for the generation of separate hydrogen and methane streams, a more economical generation of the FT syngas and reduced fouling of the FT catalyst. 1. A process for producing a synthetic hydrocarbon having a desired H/C ratio , comprising the steps ofa) subjecting organic material including biomass and microorganisms for anaerobic digestion to multiple stage anaerobic digestion for separately producing hydrogen containing biogas substantially free of methane in a first stage and methane containing biogas in a second stage, wherein to minimize growth of hydrogentrophic methanogens in the first stage, a solids retention time is decoupled from a liquid retention time in the first stage by recycling to the first stage solids separated from an effluent of the first stage,b) reforming the methane containing biogas to generate hydrogen gas and carbon monoxide gas, andc) combining the hydrogen containing biogas, the hydrogen gas, and the carbon monoxide gas into a syngas in amounts to achieve in the syngas an overall H/C ratio substantially equal to the desired H/C ratio; andd) operating a Fischer-Tropsch synthesis by reacting the syngas with a catalyst to produce the synthetic hydrocarbon.2. The process of ...

MICROALGAE-BASED SOIL INOCULATING SYSTEM AND METHODS OF USE

A system for inoculating soil with microalgae is provided. The system is portable and can be used with many different water sources to culture microalgae and form an inoculate that is added to irrigation water used for watering crops. The system provides improved crop production metrics as compared to crops grown without the microalgae-based inoculation system. The system can be integrated into existing irrigation systems to add macronutrients and micronutrients into the water, thereby providing highly bioavailable nutrients to crops. 1) A portable microalgae-based soil inoculating system comprising: a) at least one ozone source adapted to add ozone to a water source to form ozone-treated water; b) at least one solids filter adapted to remove solids from ozone-treated water to form filtered water; c) at least one carbon filter adapted to remove ozone from filtered water to form carbon filter-treated water; d) at least one UV light system to remove ozone from carbon filtered water; e) at least one microalgae-nutrient feed source adapted to add algae-nutrient feed to carbon filter-treated water to form feed water; f) at least one bioreactor adapted for receiving the feed water and cultivating microalgae and forming a microalgae-containing inoculant effluent, when a microalgae is present therein; g) at least one blower comprising a first outlet adapted to provide air to the at least one ozone source and a second outlet adapted to provide air to the at least one bioreactor; and h) at least one carbon dioxide source adapted to add carbon dioxide to air provided to the at least one bioreactor. This application is a Continuation of U.S. patent application Ser. No. 14/069,932 filed on Nov. 1, 2013, entitled “Microalgae-Based Soil Inoculating System and Methods of Use,” which is a continuation of International Patent Application No. PCT/US12/36293, filed on May 3, 2012, designating the United States of America, which claims priority to U.S. Provisional Application Ser. No. ...

INTESTINAL FLORA EXTRACTION VESSEL

The present invention provides an intestinal flora extraction vessel for extracting an intestinal flora from feces, the vessel including: a vessel main body with a first opening portion and a second opening portion formed therein, the vessel main body being configured so as to be deformable; an opening/closing mechanism configured to open/close the first opening portion of the vessel main body; a filter member accommodated in the vessel main body and partitioning the vessel main body into a first region located on the side of the first opening portion and a second region located on the side of the second opening portion, the filter member being configured to filter off the intestinal flora from the feces; and a communication member attached to the second opening portion, and configured to allow the second region to be in communication with the outside of the vessel main body. 1. An intestinal flora extraction vessel for extracting an intestinal flora from feces , the vessel comprising:a vessel main body with a first opening portion and at least one second opening portion formed therein, the vessel main body being configured so as to be deformable;an opening/closing mechanism configured to open/close the first opening portion of the vessel main body;a filter member accommodated in the vessel main body and partitioning the vessel main body into a first region located on the side of the first opening portion and a second region located on the side of the second opening portion, the filter member being configured to filter off the intestinal flora from the feces; anda communication member attached to the second opening portion, and configured to allow the second region to be in communication with the outside of the vessel main body.2. The intestinal flora extraction vessel according to claim 1 , wherein the filter member is formed in a bag shape having an internal space open to the side of the first opening portion.3. The intestinal flora extraction vessel according to ...

SYMBIOTIC ALGAE SYSTEM

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, biofuel manufacture, and desirably impacts alternative/renewable energy production, nutrient recovery from waste streams, and valued byproducts production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal-oil extraction waste or additional algae harvested as feedstock for fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as biofuels, fertilizer, etc. to compete with petroleum products in the marketplace. 1. A symbiotic algae system comprising:a first algal growth component, wherein the first algal growth component includes a heterotrophic organism, and wherein the first algal growth component produces a first effluent and an off-gas; anda second algal growth component fluidly coupled to the first algal growth component, wherein the second algal growth component includes at least one organism from the group of: a photoautotrophic organism, a mixotrophic organism, and a heterotrophic organism, andwherein the second algal growth component receives, as a first input, the first effluent and the first off-gas and produces a second effluent and a second off-gas; andwherein the second effluent and the second off-gas are received as inputs to the first algal growth component.2. A symbiotic algae system according to claim 1 , wherein the first algal growth component claim 1 , receives claim 1 , as an additional input claim 1 , an effluent input or a waste input claim 1 , and wherein the additional input and the second effluent include a nitrogen and a phosphorous.3. A symbiotic algae system according to claim 2 , wherein the first algal ...

SYSTEMS AND METHODS OF PRODUCING COMPOSITIONS FROM THE NUTRIENTS RECOVERED FROM WASTE STREAMS

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, fertilizer, feed, biofuel manufacture, and desirably impacts, nutrient recovery from waste streams for valued byproducts production, recycle water, and alternative/renewable energy production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal biomass-processing waste or additional algae harvested as feedstock for products such as fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as fertilizer, feed, biofuels, etc. to compete with non-organic or petroleum products in the marketplace. 1. A symbiotic algae system comprising:a pretreater suitable for producing a first effluent with reduced odor and biochemical oxygen demand;a first algal growth component fluidly coupled to the pretreater and receiving the first effluent, wherein the first algal growth component includes a heterotrophic algal growth strain, and wherein the first algal growth component produces a second effluent having nutrients and an off-gas; anda second algal growth component fluidly coupled to the first algal growth component, and the second algal growth component including at least one algal growth strain from the group of: a photoautotrophic algal growth strain, a mixotrophic algal growth strain, and a heterotrophic algal growth strain, andwherein the second algal growth component receives, as an input, the second effluent and the off-gas and produces a third effluent.2. A symbiotic algae system according to claim 1 , wherein the pretreater is an anaerobic digester.3. A symbiotic algae system according to claim 1 , wherein the pretreater also produces solid waste.4. A ...

Purification of Biological Molecules

The present invention relates to improved processes and systems for purification of biological molecules, where the processes can be performed in a continuous manner. 1. A process for the purification of a target molecule comprising the steps of:a) providing a sample comprising the target molecule and one or more impurities;b) adding at least one precipitant to the sample and removing one or mom impurities, thereby to recover a clarified sample;c) subjecting the clarified sample from step (b) to a bind and elute chromatography step comprising at least two separation units, thereby to obtain an eluate comprising the target molecule; andd) subjecting the eluate to flow-through purification comprising use of two or more media;wherein at least two steps are performed concurrently for at least a portion of their duration and wherein the process comprises only one bind and elute chromatography step.2. The process of claim 1 , wherein the process is a continuous process.3. The process of claim 1 , comprising a virus inactivation step between steps (c) and (d).4. The process of claim 3 , wherein the virus inactivation step comprises use of a virus inactivating agent selected from acid claim 3 , detergent claim 3 , solvent and temperature change.5. The process of claim 3 , wherein virus inactivation step comprises use of one or more in-line static mixers.6. The process of claim 3 , wherein virus inactivation comprises use of one or more surge tanks.7. The process of claim 1 , wherein the target molecule is an antibody.8. The process of claim 7 , wherein the antibody is selected from a monoclonal antibody or a polyclonal antibody.9. The process of claim 1 , wherein the precipitant in step (b) is a stimulus responsive polymer.10. The process of claim 9 , wherein the stimulus responsive polymer is a modified polyallylamine polymer.11. The process of claim 1 , wherein the precipitant in step (b) is selected from the group consisting of an acid claim 1 , caprylic acid claim 1 , a ...

Photobioreactor

A photobioreactor system includes vessel for containing a biomass and a plurality of illuminators which may have solid state devices embedded therein or coupled thereto. The light energy is distributed in the vessel volume by the illuminators so as to reach substantially all of the biomass being circulated in the vessel. Biomass may exit the top of the vessel and be refreshed with nutrients and liquid before being reintroduced at the bottom of the vessel. Carbon dioxide is introduced at the bottom of the vessel. The biomass may be agitated either ultrasonically or by motion of the illuminators. A source of energy for the light sources may be solar panels with battery storage and the carbon dioxide may be a byproduct of thermal power generation.

COMBINED ANAEROBIC DIGESTER AND GTL SYSTEM

A combined anaerobic digester system and gas-to-liquid system is disclosed. The anaerobic digester requires heat, and produces methane. The gas-to-liquid system produces heat, and converts methane to higher-value products, including methanol and formaldehyde. As such, the combination of the two systems results in significant savings in terms of capital and operating expenses. A process for producing bio-formaldehyde and bio-formalin from biogas is also disclosed. 1. A system for producing bioproducts , comprising an anaerobic digester that converts biomass to a mixture of gases comprising methane and carbon dioxide , coupled with a syngas generator capable of converting the methane to a mixture of carbon monoxide and hydrogen , and a gas-to-liquid reactor capable of converting the carbon monoxide and hydrogen to hydrocarbon products selected from the group consisting of alcohols , formaldehyde , formalin , low molecular weight (C) olefins and paraffins , and Cparaffins.2. The system of claim 1 , further comprising heat exchangers to transport heat produced in the gas-to-liquid reactor to the anaerobic digester.3. The system of claim 1 , further comprising a steam autoclave unit adapted to receive biomass claim 1 , autoclave the biomass claim 1 , and transport the biomass to the anaerobic digester.4. The system of claim 1 , further comprising a cavitation stirrer adapted to receive biomass claim 1 , and stir the biomass at extremely high speeds claim 1 , under cavitation.5. The system of claim 1 , further comprising a pressure swing absorption unit to remove carbon dioxide from the mixture of gases produced by the anaerobic digester.6. The system of claim 1 , wherein the syngas generator is an autothermal reformer or a steam reformer.7. The system of claim 1 , further comprising a reactor for growing algae or cyanobacteria claim 1 , wherein the reactor is adapted to receive carbon dioxide from the anaerobic digester and/or the gas-to-liquid reactor.8. The system of ...

Apparatus for producing organic substance from waste and method for producing organic substance from waste

The present invention provides an apparatus and a method which are suitable for producing an organic substance using a synthesis gas from a waste gasification furnace. The apparatus 1 for producing an organic substance from waste comprises a synthesis gas generation furnace 11 for generating a synthesis gas by partial oxidation of the waste; and an organic substance production unit 12 for producing an organic substance from the synthesis gas. The organic substance production unit 12 further comprises: a synthesis unit 13 for synthesizing an organic substance by subjecting the synthesis gas to catalytic reaction in the presence of a metal catalyst, and a fermenter 14 for producing an organic substance by subjecting the synthesis gas to microbial fermentation.

Systems and methods for stillage fractionation

Systems and methods for fractionating whole stillage from an ethanol production facility are provided. Whole stillage undergoes a separation of its liquid portion (thin stillage) from the solid portion (fiber cake). In some embodiments, the solids and liquids in whole stillage may be separated utilizing a screening centrifuge. The fiber cake may be dried to generate a high fiber animal feed. The thin stillage may be provided to a three-phase separator for separation into an oil emulsion, an aqueous clarified stillage, and a protein paste. The protein paste may be dried to generate a high protein animal feed with greater than about 45% protein content. The clarified thin stillage is condensed to yield a syrup with greater than around 60% solids. The oil emulsion is subjected to a pH adjustment to liberate the oil from the emulsion, which is then separated.

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. An automated stand-alone multi-module cell editing instrument for performing nuclease-directed editing in cells comprising:a housing configured to house all or some of the modules;a receptacle configured to receive the cells;one or more receptacles configured to receive expression vectors;a growth module for growing the cells for transfection;a transfection module configured to introduce the expression vectors into the cells;a nuclease-directed editing module configured to allow the introduced expression vectors to edit nucleic acids in the cells;a functional analysis module configured to conduct functional analysis of the edited cells;a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; andan automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the transfection module, from the transfection module to the nuclease-directed editing module, and from the nuclease-directed editing module to the functional analysis module without user intervention.2. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the automated liquid handling system comprises a sipper or pipettor.3. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the nuclease-directed editing module is also a recovery module.4. The automated stand-alone multi-module cell editing instrument of claim 1 , further comprising a recovery module separate from the nuclease-directed editing module.5. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the growth module is separate from the transfection module.6. The automated stand-alone multi-module cell editing instrument of claim 1 , ...

PERFUSION BIOREACTOR AND RELATED METHODS OF USE

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate. 1. A method of controlling a bioreactor system , comprising:providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI);measuring one or more process parameters (PPs) of the culture within the bioreactor by a RAMAN probe, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes;measuring a weight of the bioreactor with cell culture contents;removing cell-free spent media from the cell culture using a first output conduit at a first specified rate;removing cells from the cell culture using a second output conduit at a second specified rate;introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate; andwherein the input and output conduits are adjusted based on the RAMAN probe measurements and weight measurement of the bioreactor to maintain (i) one or more of the process parameters within predetermined ranges, (ii) the weight of the bioreactor with the cell culture within a predetermined range, and (iii) the third ...

METHOD FOR CONVERTING BIOMASS TO METHANE

A method for enhancing the treatment of lignocellulose-containing materials by biotreatment wherein such lignocellulose-containing materials, normally resistant to biotreatment, are first subjected to a low-temperature, long-residence time pyrolysis at about 175° C. to about 325° C. for about 0.1 hour to about 2.0 hours, wherein a substantial portion of the incoming material is distilled into water-soluble compounds amenable to anaerobic biotreatment. Exemplary applications of the method include pyrolytic pre-treatment of wastewater sludges, cellulosic wastes, wood, peat, plant residues, low-grade coal, and the like to enhance methane gas production in anaerobic digestion and/or oxygen-limited or oxygen-starved fermentation to produce ethanol. 1. A system comprising ,a pyrolizer having an inlet and an outlet,a condenser having an inlet, a non-condensable gas outlet and a liquid outlet, andan anaerobic digester having one or more inlets,wherein the outlet of the pyrolizer is connected to the inlet of the condenser, and the non-condensable gas outlet or the condenser is connected to an inlet of the anaerobic digester.2. The system of further comprising a phase separator having an inlet claim 1 , an aqueous phase outlet and an organic phase outlet claim 1 , wherein the phase separator inlet is connected to the liquid outlet of the condenser and the aqueous phase outlet of the phase separator is connected to an inlet of the anaerobic digester.3. The system of wherein the pyrolizer comprises an internal screw.4. A process comprising the steps of claim 1 ,subjecting one or more biomaterials to pyrolysis to produce pyrolysis products including at least a pyrogas and char, andmixing the pyrogas into a sludge in an anaerobic digester.5. The process of wherein the one or more biomaterials comprise anaerobic digester sludge and undigested lignocellulosic material.6. The process of wherein the one or more biomaterials comprise municipal solid waste.7. The process of wherein the ...

Methods and systems for supplying hydrogen to a hydrocatalytic reaction

Systems and methods involving hydrocatalytic reactions that use molecular hydrogen obtained from a biogas generated from at least a portion of the hydrocatalytic reaction product. Hydrocatalytic reactions can require significant quantities of molecular hydrogen, particularly if the molecular hydrogen is being introduced under dynamic flow conditions. The present disclosure provides systems and methods that can allow for reducing the carbon footprint of the fuels formed from the hydrocatalytic reaction because at least a portion of the hydrogen used in the hydrocatalytic reaction has low carbon footprint. A fuel with low carbon footprint can qualify for certain governmental status that provides certain benefits.

COMPOSITIONS, METHODS, MODULES AND INSTRUMENTS FOR AUTOMATED NUCLEIC ACID-GUIDED NUCLEASE EDITING IN MAMMALIAN CELLS VIA VIRAL DELIVERY

This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells. 1. A method of transducing , transfecting , and performing nucleic acid-guided nuclease editing in mammalian cells in an automated closed editing instrument comprising the steps of:providing an automated closed cell editing instrument comprising a growth module and a liquid handling system;transferring cell growth medium and mammalian cells to the growth module via the liquid handling system;growing mammalian cells into aggregates in the growth module;passaging the mammalian cells into smaller aggregates when the aggregates exceed 50-300 microns in size;synthesizing and amplifying a library of editing cassettes off-instrument, wherein each editing cassette comprises a different gRNA and donor DNA pair;inserting editing cassettes into a viral backbone and producing viral particles off-instrument;delivering the viral particles to the mammalian cells in the growth module at a multiplicity of infection (MOI) such that each mammalian cell receives one viral particle or no viral particle;providing conditions to allow the viral vector to integrate into the mammalian cell DNA;enriching for mammalian cells with an integrated viral vector;delivering a nucleic acid-guided nuclease or nuclease fusion or a coding sequence for a nucleic acid-guided nuclease or nuclease fusion synthesized off-instrument to the enriched mammalian cells in the growth module via the liquid handling system; andproviding conditions to allow editing to take place in the mammalian cells.2. The method of claim 1 , wherein the growth module is a rotating growth module.3. The method of claim 1 , wherein the growth module is a tangential flow filtration module.4. The method of claim 1 , wherein the growth module is a bioreactor.5. The method of claim 1 , wherein the automated closed cell ...

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. An automated stand-alone multi-module cell editing instrument for performing nuclease editing comprising:a receptacle configured to receive cells and/or nucleic acids;a growth module for growing the cells for transformation;a cell concentration module to concentrate the cells and render the cells electrocompetent;a transformation module configured to introduce nucleic acids into the cells;a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells;a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script, wherein the processor comprises a robotics controller, module controller, temperature controller, and a CPU; andan automated liquid handling system to move liquids from the receptacle to the growth module, from the growth module to the cell concentration module, from the cell concentration module to the transformation module, from the transformation module to the nuclease-directed editing module, without user intervention.2. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the nuclease-directed editing module is also a selection module.3. The automated stand-alone multi-module cell editing instrument of claim 1 , further comprising a selection module.4. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the processor also comprises a graphical user interface.5. The automated stand-alone multi-module cell editing instrument of claim 4 , wherein the graphical user interface comprises one or more data entry panes for one or more of the growth module claim 4 , the cell concentration module claim 4 , the transformation module and the nuclease-directed editing module.6. The automated ...

MICROBIAL HUMIC SOIL ENHANCEMENTS

A method enhances soil by preparing a microbial solution with microbes, a growth medium, and water; iteratively and selectively breeding generations of microbes to arrive at a predetermined microbial solution in a concentrated form of at least 1×10cfu/ml (colony-forming units per milliliter); adding humic acid with amino acids and protein to support an active microbial population to support active and healthy plant growth; and storing the microbial solution as a solid for enriching the soil with micronutrients, microbial cultures and organic materials. 1. A method for enhancing soil , comprising:preparing a microbial solution with microbes, a growth medium, and water;{'sup': '7', 'iteratively and selectively breeding generations of microbes to arrive at a predetermined microbial solution in a concentrated form of at least 1×10cfu/ml (colony-forming units per milliliter);'}adding humic acid with amino acids and protein to support an active microbial population to support active and healthy plant growth; andstoring the microbial solution in a container for enriching the soil with micronutrients, microbial cultures and organic materials.2Bacillus. The method of claim 1 , comprising selecting a member of as the microbe and providing a carrier from one of: liquid claim 1 , water claim 1 , dry humic acid claim 1 , wet humic acid claim 1 , urea claim 1 , or a penetrant.3. The method of claim 1 , wherein the growth medium comprises a carbon source.4. The method of claim 1 , wherein the growth medium comprises sugar claim 1 , molasses claim 1 , or maltodextrin.5. The method of claim 1 , comprising mixing the solution with 1 part microbes claim 1 , 10 part carbon source claim 1 , and 1000 parts water.6. The method of claim 1 , comprising aerating the solution for—at least 20 minutes before applying to the soil.7BacillusBacidiceler, B. acidicola, B. acidiproducens, B. acidocaldarius, B. acidoterrestrisr, B. aeolius, B. aerius, B. aerophilus, B. agaradhaerens, B. agri, B. ...

SOIL ENHANCEMENT

Systems and methods are disclosed to provide a plant nutrient by selecting a microbial solution with predetermined characteristics for agriculture use; iteratively and selectively breeding generations of microbes for microbial strain selection with predetermined microbial gene profiles to arrive at a predetermined microbial solution in a highly concentrated form of at least 1×10cfu/ml (colony-forming units per milliliter), wherein multiple single microbial series are separately cultivated and followed with cross cultivation among the microbial series in a specific sequence; and mixing by-products produced by the crossly cultivated microbial series with humic acid and a filler. 1. A method to provide plant nutrient , comprising:selecting a microbial solution with predetermined characteristics for agriculture use;{'sup': '7', 'iteratively and selectively breeding generations of microbes for microbial strain selection with predetermined microbial gene profiles to arrive at a predetermined microbial solution in a highly concentrated form of at least 1×10cfu/ml (colony-forming units per milliliter), wherein multiple single microbial series are separately cultivated and followed with cross cultivation among the microbial series in a specific sequence; and'}mixing by-products produced by the crossly cultivated microbial series with humic acid and a filler.2. The method of claim 1 , comprising applying kelp as the filler.3. The method of claim 1 , comprising mixing fertilizer with the by-products.4. The method of claim 1 , comprising mixing nitrogen phosphorous and potassium (NPK) with the by-products.5. The method of claim 1 , comprising:providing a liquid to the solid during use and binding the mixed by-products including humic acid to soil; andminimizing chemical runoff with the humic bound soil.6. The method of claim 1 , comprising forming a solid from the mixed by-products.7. The method of claim 1 , comprising forming pellets from the mixed by-products.8. The method of ...

Automated cell processing methods, modules, instruments, and systems

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

CONVERSION OF BIOMASS, ORGANIC WASTE AND CARBON DIOXIDE INTO SYNTHETIC HYDROCARBONS

A process and system for producing a synthetic hydrocarbon having a desired H/C ratio is disclosed. Organic material is biochemically digested in a two stage biodigester for separately producing a hydrogen containing biogas substantially free of methane in a first stage and a methane containing biogas in a second stage. The methane containing biogas is reformed in a first reformer to generate hydrogen gas and carbon monoxide gas, which are then combined in a mixer with the hydrogen containing biogas into a syngas in amounts to achieve in the syngas an overall H/C ratio substantially equal to the desired H/C ratio. The syngas is reacted with a catalyst in a second reformer, a Fischer-Tropsch (FT) reactor, to produce the hydrocarbon. Using a two stage biodigester allows for the generation of separate hydrogen and methane streams, a more economical generation of the FT syngas and reduced fouling of the FT catalyst. 1. A process for producing a synthetic hydrocarbon having a desired H/C ratio , comprising the steps ofa) biochemically digesting organic material for separately producing a hydrogen containing biogas substantially free of methane and a methane containing biogas,b) reforming the methane containing biogas to generate hydrogen gas and carbon monoxide gas, andc) combining the hydrogen containing biogas, the hydrogen gas, and the carbon monoxide gas into a syngas in amounts to achieve in the syngas an overall H/C ratio substantially equal to the desired H/C ratio; andd) operating a Fischer-Tropsch synthesis by reacting the syngas with a catalyst to produce the synthetic hydrocarbon.2. The process of claim 1 , wherein in step a) the organic material includes biomass and microorganisms for anaerobic digestion and the biochemically digesting includes subjecting the mixture to multiple stage anaerobic digestion for producing the hydrogen containing biogas in a first stage and the methane containing biogas in a second stage.3. The process of claim 2 , wherein the ...

Integrated Biorefinery

Integrated biorefineries are provided that integrate biomass cultivation with processing to convert cultivated biomass to a fuel, and further integrate carbon recovery from the biomass processing. Biomass processing to fuels begins in a treatment system, such as a hydrothermal treatment system, that produces an organic phase that is suitable for refining to a fuel and also produces a waste stream. The biorefinery optionally includes a dewatering system, a refining system to convert the organic phase to the fuel, and a cogeneration system configured to use at least some of the fuel produced by the treatment system to generate electricity and heat that can be used for biorefinery operations.

SYSTEMS AND METHODS FOR STILLAGE FRACTIONATION

Systems and methods for fractionating whole stillage from an ethanol production facility are provided. Whole stillage undergoes a separation of its liquid portion (thin stillage) from the solid portion (fiber cake). In some embodiments, the solids and liquids in whole stillage may be separated utilizing a screening centrifuge. The fiber cake may be dried to generate a high fiber animal feed. The thin stillage may be provided to a three-phase separator for separation into an oil emulsion, an aqueous clarified stillage, and a protein paste. The protein paste may be dried to generate a high protein animal feed with greater than about 45% protein content. The clarified thin stillage is condensed to yield a syrup with greater than around 60% solids. The oil emulsion is subjected to a pH adjustment to liberate the oil from the emulsion, which is then separated. 119-. (canceled)20. A method for processing whole stillage in an ethanol plant , comprising:a) separating thin stillage liquid from the whole stillage;b) separating protein paste and clarified liquid stillage output from the thin stillage liquid; andc) sending at least a portion of the clarified liquid stillage output to an evaporation system to concentrate the clarified liquid stillage output into a concentrated, clarified liquid stillage having at least 60% solids.21. The method of claim 20 , wherein separating the thin stillage liquid from the whole stillage produces a fiber cake.22. The method of claim 21 , further comprising drying the fiber cake to provide a high fiber animal feed product.23. The method of claim 20 , further comprising drying protein paste to provide a high protein animal feed product.24. The method of claim 20 , wherein the concentrated claim 20 , clarified liquid stillage is used as an animal feed supplement.25. The method of claim 20 , further comprising recycling a portion of the clarified liquid stillage output upstream for utilization as fermentation backset.26. The method of claim 20 , ...

BIOPROCESS AND MICROBE ENGINEERING FOR TOTAL CARBON UTILIZATION IN BIOFUEL PRODUCTION

Some aspects of this invention provide methods and bioreactors for converting a carbon source into a lipid. In some embodiments, lipid production is carried out in an aerobic fermentor and carbon dioxide generated during lipid production is converted into a carbon substrate by COfixation in an anaerobic fermentor. In some embodiments, the carbon substrate generated by COfixation is used as the carbon source for lipid production, thus achieving total carbon utilization in lipid production. 1. A method comprising{'sub': '2', '(a) culturing in a liquid medium a first organism in the presence of a carbon source under aerobic conditions suitable for the first organism to oxidize the carbon source wherein the conditions are oxidizing and conditions, wherein the first organism produces COas part of the oxidation process; and'}{'sub': 2', '2, '(b) culturing in a liquid medium a second organism in the presence of COproduced in (a) under anaerobic conditions suitable for the second organism to reduce the CO, wherein the conditions are reducing and conditions, wherein the second organism produces a carbon substrate as part of the reduction process.'}26.-. (canceled)7. The method of claim 1 , wherein the culturing of (a) and of (b) is carried out in separate fermentors.8. (canceled)9. (canceled)10. The method of claim 1 , further comprising contacting the first organism with an oxidizing agent claim 1 , optionally wherein the oxidizing agent is O.11. (canceled)12. The method of claim 1 , further comprising contacting the second organism with a reducing agent claim 1 , optionally wherein the reducing agent is H claim 1 , CO claim 1 , syngas claim 1 , or HS.1316.-. (canceled)17. The method of claim 1 , further comprising providing electrons to the second organism of (b) by contacting the second organism of (b) with an electric current.18. (canceled)19. The method of claim 1 , wherein the carbon source is a carbohydrate claim 1 , optionally glucose claim 1 , fructose claim 1 , ...

BIOREACTOR USING ACOUSTIC STANDING WAVES

A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor. 1. A system comprising:a bioreactor;an acoustophoretic separator fluidly connected to the bioreactor; anda filter fluidly connected to the acoustophoretic separator.2. The system of claim 1 , wherein the filter is a depth filter.3. The system of claim 1 , wherein the acoustophoretic separator comprises at least two serially coupled flow chambers.4. The system of claim 1 , wherein the bioreactor comprises a flexible bag.5. The system of claim 1 , further comprising at least one separation column fluidly connected to the filter and arranged to receive a filtrate or a permeate from the filter.6. The system of claim 1 , wherein the acoustophoretic separator includes an ultrasonic transducer and a reflector located opposite the ultrasonic transducer that are configured to produce a multi-dimensional standing wave when the ultrasonic transducer is actuated.7. The system of claim 1 , further comprising a recycle path fluidly connected between the bioreactor and one or more of the acoustophoretic separator or the filter.8. A process claim 1 , comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'providing the system of ;'}introducing a cell culture medium and cells into the bioreactor;cultivating the cells in the bioreactor; andwithdrawing a filtrate or a permeate via the acoustophoretic separator and the filter.9. The process of claim 8 , further comprising recycling a fluid from either the acoustophoretic separator or the filter claim 8 , back to the bioreactor.10. A system comprising:a bioreactor;an acoustophoretic separator fluidly connected to the bioreactor; andat least one separation column fluidly connected to the acoustophoretic separator.11. The ...

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. A method for editing nucleic acids in live cells in an automated stand-alone multi-module cell editing instrument , comprising the steps of:providing live cells in a receptacle configured to receive the live cells;providing first nucleic acids to one or more receptacles configured to receive first nucleic acids;growing the live cells in a growth module to a desired optical density to produce grown live cells;transforming the grown live cells in a transformation module configured to introduce the first nucleic acids into the grown live cells to produce transformed cells;providing conditions in a nuclease-directed editing module to allow the first nucleic acids to edit nucleic acids in the transformed cells to produce edited cells; andusing an automated liquid handling system to 1) transfer the first nucleic acids from the one or more receptacles configured to receive nucleic acids to the transformation module, 2) transfer the live cells from the receptacle configured to receive the live cells to the growth module, 3) transfer the grown live cells from the growth module to the transformation module, and 4) transfer the transformed cells from the transformation module to the nuclease-directed editing module without user intervention.2. (canceled)3. The method of editing live cells according to claim 1 , further comprising the step of assembling nucleic acids in a nucleic acid assembly module.4. The method of editing live cells according to claim 1 , wherein the transformation module comprises a flow-through electroporation device.5. The method of editing live cells according to claim 1 , wherein the growth module comprises a rotating growth vial.6. The method of editing live cells according to claim 1 , wherein the growth module further comprises a Peltier device.7. The method of editing live ...

Solar steam systems for optimized algal biomass processing

Systems and methods for algae processing and, more particularly, to systems and methods for having integrated solar steam systems. Trapped heated air accumulated within the solar steam system, such as a greenhouse-enclosed solar steam system, is swept over a cultivated algae slurry in order to facilitate drying thereof and increasing the thermal efficiency of a biofuel algae facility.

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. An automated stand-alone multi-module cell editing instrument for performing RNA-guided nuclease-directed editing in target regions in cells comprising:a housing configured to house all or some of the modules;a receptacle configured to receive the cells;one or more receptacles configured to receive expression vectors, wherein the expression vectors introduce a desired DNA change to the target region and remove a proto-spacer motif (PAM) region from the target region;a growth module for growing the cells for transfection;a transformation module configured to introduce the expression vectors into the cells;an RNA-guided nuclease-directed editing module configured to allow the introduced expression vectors to edit nucleic acids in the target region in the cells;a selection module configured to select cells with a desired edit using the RNA-guided nuclease complexed with an gRNA complementary to the target region of the edited cells;a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; andan automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the transformation module, from the transformation module to the RNA-guided nuclease-directed editing module, and from the RNA-guided nuclease-directed editing module to the selection module without user intervention.2. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the RNA-guided nuclease is MAD7 nuclease.3. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the RNA-guided nuclease-directed editing module is also a recovery module.4. The automated stand-alone multi-module cell editing instrument of claim 1 , further comprising ...

METHODS AND SYSTEMS FOR PRODUCING PRODUCTS USING ENGINEERED SULFUR OXIDIZING BACTERIA

Methods and systems for producing a biofuel using genetically modified sulfur-oxidizing and iron-reducing bacteria (SOIRB) are disclosed. In some embodiments, the methods include the following: providing a SOIRB that have been genetically modified to include a particular metabolic pathway to enable them to generate a biofuel; feeding a first source of ferric iron to the SOIRB; feeding sulfur, water, and carbon dioxide to the SOIRB; producing at least the first particular biofuel, a first source of ferrous iron, sulfate, excess ferric iron, and an SOIRB biomass; electrochemically reducing the excess ferric iron to a second source of ferrous iron; providing an iron-oxidizing bacteria that have been genetically modified to include a particular metabolic pathway to enable them to generate a second biofuel; producing at least the second biofuel, a second source of ferric iron, and an IOB biomass; and feeding the second source of ferric iron to the SOIRB. 1. A method for producing a biofuel using genetically modified bacteria , said method comprising:providing in a first bioreactor bacteria that is genetically modified to oxidize elemental sulfur and reduce iron (SOIRB), wherein said SOIRB includes a valine biosynthesis metabolic pathway that produces 2-keto acids and is genetically modified to include a 2-keto-acid decarboxylase gene and an alcohol dehydrogenase gene, said SOIRB being genetically modified so that said genes in said SOIRB convert said 2-keto acids to isobutanol;feeding a source of ferric iron to said SOIRB;feeding sulfur, water, and carbon dioxide to said SOIRB;oxidizing said sulfur;reducing said first source of ferric iron;producing at said first bioreactor at least one of biofuel, a source of ferrous iron, sulfate, excess ferric iron, and an SOIRB biomass;providing in a second bioreactor an iron-oxidizing bacteria (IOB), said IOB includes a valine biosynthesis metabolic pathway that produces 2-keto acids and is genetically modified to include a 2-keto- ...

METHOD AND SYSTEM OF PROCESSING MEAL FROM OILSEEDS

The method and system for processing meal from oilseeds after oil extraction includes biotransformation of meal and its cascade, sequential refining leading to the separation of the product or products with high added value, and finally obtaining the remaining biomass with an increased nutritional value compared to the original post-extraction meal material. 1. A method of processing meal obtained from oilseed after oil extraction using biotransformation , with microorganisms employed in the solid or liquid phase of fermentation , the method comprising:hydrating oilseed obtained meal and additives stimulating the biosynthesis of products with water from the water tank to water content of 20% to 85%, and{'sub': '2', 'inoculating with microorganisms, while the oilseed obtained meal is pre-treated in a meal tank, which involves its sterilization, and then dosing into the bioreactor, where it is incubated from 6 h to 48 h at 20° C. to 50° C. and, after the fermentation, the mixture is directed to the separator where solid fraction is separated from the liquid fraction, while the solid fraction is then dried at a temperature not exceeding the denaturalization temperature of enzymes, and then dispensed into the supplementary feed mix component tank, whereas the liquid fraction is directed to a the centrifuge, in which microorganism cells and solid impurities of diameter sizes greater than 0.1 μm are centrifuged and directed back to the bioreactor and the purified liquid fraction is directed, either according to the first option, into the absorber, in which sorption of bio surfactants produced by microorganisms is carried out on activated carbon, desorption of bio surfactants produced by microorganisms is carried out by subjecting them to COextraction under supercritical conditions of activated carbon with adsorbed bio surfactants, in the pressure reactor, which has previously been dried in the dryer or, in accordance with the second option, is extracted in the reactor at ...

MULTI-FUNCTIONAL PORT FOR MICROALGAE CULTIVATION AND HARVESTING

The present invention relates to a port installed in a closed-ended photochemical reactor, and more particularly, to a multi-functional port for microalgae cultivation and harvesting that supplies gas for cultivation for culturing microalgae by connecting to the closed-ended photochemical reactor; supplies gas for prevention of precipitation for lifting microalgae during cultivation; and assembles by taking samples of microalgae or selectively combining a plurality of valves which can harvest microalgae, whose cultivation is completed. 1. A multi-functional port for microalgae cultivation and harvesting comprising: a first one touch valve , T-shaped , for supplying each of gas for cultivation for culturing microalgae , which come from a gas supply tube line for cultivation , and gas for prevention of precipitation for lifting microalgae , which come from a gas supply tube line for prevention of precipitation , to the inside of a closed-ended photochemical reactor by inserting a gas supply tube for cultivation and a gas supply tube for prevention of precipitation to the inside of the closed-ended photochemical reactor , and for taking samples of microalgae cultured in the closed-ended photochemical reactor or discharging all microalgae to sampling and harvesting tube lines through the gas supply tube for prevention of precipitation;a second one touch valve, T-shaped, in which one end is connected to the first one touch valve with a connection tube; the other end is connected to the gas supply tube line for prevention of precipitation, thereby intermittently supplying the gas for prevention of precipitation to the first one touch valve; and microalgae is released from the first one touch valve to the other end, thereby being discharged to the sampling and harvesting tube lines; anda one touch hand valve for harvesting, I-shaped, in which one end is connected to the first one touch valve or the second one touch valve with the connection tube; and the other end is ...

Microalgae-based Soil Inoculating System and Methods of Use

A system for inoculating soil with microalgae is provided. The system is portable and can be used with many different water sources to culture microalgae and form an inoculate that is added to irrigation water used for watering crops. The system provides improved crop production metrics as compared to crops grown without the microalgae-based inoculation system. The system can be integrated into existing irrigation systems to add macronutrients and micronutrients into the water, thereby providing highly bioavailable nutrients to crops.

Production of hydrogen using an anaerobic biological process

Various embodiments of the present invention pertain to methods for biological production of hydrogen. More specifically, embodiments of the present invention pertain to a modular energy system and related methods for producing hydrogen using organic waste as a feed stock.

METHOD AND SYSTEM FOR CONVERTING ELECTRICITY INTO ALTERNATIVE ENERGY RESOURCES

A method of using electricity to produce methane includes maintaining a culture comprising living methanogenic microorganisms at a temperature above 50° C. in a reactor having a first chamber and a second chamber separated by a proton permeable barrier, the first chamber comprising a passage between an inlet and an outlet containing at least a porous electrically conductive cathode, the culture, and water, and the second chamber comprising at least an anode. The method also includes coupling electricity to the anode and the cathode, supplying carbon dioxide to the culture in the first chamber, and collecting methane from the culture at the outlet of the first chamber. 1. A method of using electricity to produce methane , the method comprising:maintaining a culture comprising living methanogenic microorganisms at a temperature above 50° C. in a reactor having a first chamber and a second chamber separated by a proton permeable barrier, the first chamber comprising a passage between an inlet and an outlet containing at least a porous electrically conductive cathode, the culture, and water, and the second chamber comprising at least an anode;coupling electricity to the anode and the cathode;supplying carbon dioxide to the culture in the first chamber; andcollecting methane from the culture at the outlet of the first chamber.2. The method of claim 1 , comprising circulating an aqueous electrolytic medium through the passage and the cathode.3. The method of claim 2 , wherein the passage is formed between the proton permeable barrier and a current collector.4. The method of claim 2 , wherein the first chamber consists essentially of the porous electrically conductive cathode disposed in the passage between the inlet and the outlet claim 2 , the culture comprising living methanogenic microorganisms claim 2 , and the aqueous electrolytic medium circulating through the cathode.5. (canceled)6. The method of claim 1 , wherein (i) the proton permeable barrier comprises a solid ...

METHODS AND SYSTEMS FOR THE PRODUCTION OF ALCOHOLS AND/OR ACIDS

Methods and systems for the production of one or more products from a gas stream produced in a methanol production process. The method comprises converting at least a portion of a methane feedstock to a substrate comprising CO and H2. The substrate comprising CO and H2 is anaerobically fermented in a bioreactor to produce one or more alcohols. The method and system may further include process for the production of methanol processes for the production of acetic acid. 1. A method for producing one or more alcohols the method comprising:a. passing one or more products and/or by-products and/or waste products from a methanol carbonylation process to a bioreactor containing a culture of one or more microorganisms; andb. fermenting the culture in the bioreactor to produce one or more alcohols.2. The method of wherein a substrate comprising methanol and/or carbon monoxide is provided to the methanol carbonylation process from a methanol synthesis reaction.3. The method of wherein products and/or by-products and/or waste products of the methanol carbonylation process comprise at least one compound selected from the group comprising CO claim 1 , N2 claim 1 , CO2 claim 1 , CH4 claim 1 , methanol and acetic acid.4. A system for the production of one or more alcohols claim 1 , the system comprising:a. a methanol carbonylation vessel configured to convert methanol and CO to one or more products and/or by-products and/or waste products;b. a bioreactor containing a culture of one or more microorganisms, the bioreactor being configured to convert at least a portion of the one or more products and/or by-products and/or waste products to one or more alcohols by microbial fermentation; andc. a means for passing the one or more products and/or by-products and/or waste products from the methanol carbonylation vessel to the bioreactor.5. The system of wherein the methanol carbonylation vessel is configured to receive at least a portion of the methanol and/or CO from a methanol synthesis ...

INTEGRATED FERMENTATION AND ELECTROLYSIS PROCESS

The invention provides schemes for the integration of a fermentation process, with an electrolysis process, and a C1-generating industrial process. In particular, the invention provides process for utilizing electrolysis products, for example Hand/or O, to improve the process efficiency of at least one of the fermentation process or the C1-generating industrial process. More particularly, the invention provides a process whereby, Hgenerated by electrolysis is used to improve the substrate efficiency for a fermentation process, and the Ogenerated by the electrolysis process is used to improve the composition of the C1-containing tail gas generated by the C1-generating industrial process. 1. A process for improving carbon capture efficiency in an integrated fermentation and industrial process , wherein the process comprises:i. passing one or more feedstocks to an electrolysis process to produce an electrolysis derived substrate;ii. blending at least a portion of the electrolysis derived substrate with a C1-containing tail gas from an industrial process to provide a blended C1-containing substrate;iii. passing the blended C1-containing substrate to a bioreactor containing a culture of at least one C1-fixing bacterium; andiv. fermenting the culture to produce one or more fermentation products.2. The process of claim 1 , wherein{'sub': 2', '2, 'i. COis passed to an electrolysis process to produce an electrolysis derived substrate comprising CO and O;'}ii. the industrial process produces a C1-containing tail gas; andiii. at least a portion of the CO from (i) is blended with at least a portion of the C1-containing tail gas from (ii) to provide the blended C1-containing substrate.3. The process of claim 2 , wherein at least a portion of the Ofrom (i) is passed to the industrial process.4. The process of claim 2 , wherein an exit gas stream is produced by the fermentation claim 2 , the exit gas comprises CO claim 2 , and at least a portion of the COis recycled to the ...

Integrated manufacturing and chromatographic system for virus production

Provided is a method for producing and/or purifying measles virus (MV) particles from a sample, the method comprising in sequential order the following steps loading a sample containing MV particles and one or more impurities onto a stationary phase material for carrying out flow-through chromatography to bind at least a fraction of the impurities contained in the sample and to produce a flow-through comprising at least a fraction of the MV particles contained in the sample; carrying out filtration, preferably ultrafiltration, and obtaining a retentate having an increased MV titer relative to the MV titer comprised in the flow-through. Further provided is a system for producing and/or purifying MV particles, comprising at least one bioreactor; a clarification unit, preferably a dead end filter unit, downstream to the bioreactor; a flow through chromatography unit downstream to the clarification unit; and a filtration unit, downstream to the flow through chromatography unit.

Solid gel amplification method and apparatus for platform molecular diagnostics

The present invention provides for a novel system and method for amplification and detection of nucleic acids within a miniaturized device wherein sample administration occurs via capillary forces through a channel created by drying a hydrogel containing all components needed for a cell-free, enzymatic, nucleic-acid amplification system other than the template nucleic acid or precursor thereto, and wherein an aqueous sample is provided to the desiccated hydrogel, and the hydrogel is rehydrated, through capillary forces.

REFUSE TREATMENT METHOD AND APPARATUS FOR SEPARATING SOLID AND LIQUID AND SEPARATING ORGANICS AND INORGANICS

A refuse treatment method for separating solid and liquid and separating organics and inorganics, comprising the steps of: 1) subjecting municipal domestic refuse to a bag-breaking treatment and water washing; 2) washing the municipal domestic refuse after the bag-breaking treatment with water; 3) performing a solid liquid separation treatment on the washed municipal domestic refuse; 4) performing a cracking treatment and then a dehydration treatment on sorted water-insoluble organic refuse; 5) performing dehydration on marsh gas residue after marsh gas is generated in the refuse water to produce biochar. This application also discloses an apparatus for implementing the method described above. 1. A refuse treatment method for separating solid and liquid and separating organics and inorganics , comprising the steps of:1) subjecting municipal domestic refuse to a bag-breaking treatment and water washing;2) performing a washing treatment on the municipal domestic refuse after the bag-breaking treatment with water to wash off water-soluble organics in the municipal domestic refuse;3) performing a solid liquid separation treatment on the washed municipal domestic refuse; performing a sorting treatment on separated solid refuse; performing an anaerobic treatment on separated refuse water after precipitation to convert organics in the refuse water to marsh gas, and utilizing clear water after a cleaning treatment; andcontinuing to perform spray washing on the solid refuse in the sorting treatment, performing an anaerobic treatment on washed-off refuse water after precipitation to convert organics in the refuse water to marsh gas, and utilizing clear water after a cleaning treatment;4) performing a cracking treatment and then a dehydration treatment on sorted water-insoluble organic refuse, performing a pyrolysis treatment in a hypoxic state to produce biochar, performing an anaerobic treatment on sewage generated during the dehydration treatment to convert organics in the ...

MICROBIAL SOIL ENHANCEMENT

A method enhances soil by preparing a microbial solution with microbes, a growth medium, and water; iteratively and selectively breeding generations of microbes to arrive at a predetermined microbial solution in a concentrated form of at least 1×10cfu/ml (colony-forming units per milliliter); adding humic acid with amino acids and protein to support an active microbial population to support active and healthy plant growth; and storing the microbial solution as a solid for enriching the soil with micronutrients, microbial cultures and organic materials. 1. A method for enhancing soil , comprising:preparing a microbial solution with microbes, a growth medium, and water;{'sup': '7', 'iteratively and selectively breeding generations of microbes to arrive at a predetermined microbial solution in a concentrated form of at least 1×10cfu/ml (colony-forming units per milliliter);'}adding humic acid with amino acids and protein to support an active microbial population to support active and healthy plant growth; andstoring the microbial solution in a container for enriching the soil with micronutrients, microbial cultures and organic materials.2Bacillus. The method of claim 1 , comprising selecting a member of as the microbe and providing a carrier from one of: liquid claim 1 , water claim 1 , dry humic acid claim 1 , wet humic acid claim 1 , urea claim 1 , or a penetrant.3. The method of claim 1 , wherein the growth medium comprises a carbon source.4. The method of claim 1 , wherein the growth medium comprises sugar claim 1 , molasses claim 1 , or maltodextrin.5. The method of claim 1 , comprising mixing the solution with 1 part microbes claim 1 , 10 part carbon source claim 1 , and 1000 parts water.6. The method of claim 1 , comprising aerating the solution for—at least 20 minutes before applying to the soil.7BacillusBacidiceler, B. acidicola, B. acidiproducens, B. acidocaldarius, B. acidoterrestrisr, B. aeolius, B. aerius, B. aerophilus, B. agaradhaerens, B. agri, B. ...

SYMBIOTIC ALGAE SYSTEM

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, biofuel manufacture, and desirably impacts alternative/renewable energy production, nutrient recovery from waste streams, and valued byproducts production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal-oil extraction waste or additional algae harvested as feedstock for fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as biofuels, fertilizer, etc. to compete with petroleum products in the marketplace. 1. A symbiotic algae system for removing nutrients from an input stream including organic carbon waste matter and which is either phosphorus rich or nitrogen rich , the symbiotic algae system comprising:a first algal growth component, wherein the first algal growth component includes a first container suitable for growing a first heterotrophic algae strain, and wherein the first algal growth component produces a first algal biomass, a first effluent including a portion of the organic carbon waste matter and a first growth byproduct, and a carbon dioxide rich off-gas; anda second algal growth component fluidly coupled to the first algal growth component, the second algal growth component including a second container suitable for growing a second algal strain, wherein the second algal strain is a phototrophic or mixotrophic algal strain that is different strain than the first heterotrophic algae strain, and wherein the second algal growth component produces a second algal biomass, a second effluent including a smaller portion of the organic carbon waste matter, a second growth byproduct, and an oxygen rich off-gas, wherein the second ...

PROCESS FOR PRODUCTING AN ORGANIC PRODUCT FROM A CARBON-BASED MATTER FEEDSTOCK USING GASIFICATION FOLLOWED BY FERMENTATION OF THE SYNTHESIS GAS

The invention relates to a process for producing a fuel, in particular a liquid fuel, or another organic product, from a carbon-based matter feedstock, comprising the following steps: a/ gasification of the carbon-based matter feedstock in a first reactor, termed gasifier (), b/ downstream of the gasification, fermentation of the synthesis gas produced according to step a/, by means of microorganisms, water and nutrients in a second reactor, termed fermenter (), c/ recovery, downstream of the fermenter, of the microorganisms and of the water, d/ injection of at least a part of the recovered microorganisms and, where appropriate, of at least a part of the recovered water at the inlet () of the gasifier. 112-. (canceled)13. A process for the production of a fuel , in particular a liquid fuel , or of another organic product , from a carbon-based matter feedstock , comprising the following stages:a/ gasification of the carbon-based matter feedstock in a first reactor, referred to as gasifier,b/ downstream of the gasification, fermentation of the synthesis gas produced according to stage a/ using microorganisms, water and nutrients in a second reactor, referred to as fermenter,c/ recovery, downstream of the fermenter, of the microorganisms and water, a stage c1/ of separation between the microorganisms which have been used for the fermentation and the mixture between the organic product resulting from the fermentation and the water, followed by a distillation of the mixture in order to produce the final organic product,', 'a stage c2/ of adjustment of the concentration of water in the microorganisms recovered by the separation., 'd/ injection of at least a portion of the recovered microorganisms and, if appropriate, of at least a portion of the recovered water at the inlet of the gasifier, and, during the recovery stage c/14. The production process as claimed in claim 13 , according to which claim 13 , between the gasification stage a/ and the fermentation stage b/ claim ...

Recovery of higher alcohols from dilute aqueous solutions

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

VARIABLE DIAMETER BIOREACTORS

A variable diameter bioreactor vessel is provided that includes a first vessel section having a first diameter configured to hold a liquid media and biologic material, and a second vessel section having a second diameter that is greater than the first diameter such that the liquid media can be increased from a first volume to a second volume within the vessel. 12-. (canceled)3. A variable diameter bioreactor vessel configured for mammalian cell production , comprising:a first vessel section having a first diameter configured to hold a liquid media and biologic material;said first vessel section having a conical design such that the base of said first vessel section is narrower than the top of said first vessel section;a second vessel section wherein the diameter of the bottom of said second vessel section is the same as that of the top of said second vessel section; andwherein said second vessel section is situated such that the liquid media and biologic material can be increased from a first volume to a second volume within the vessel.4. The variable diameter bioreactor vessel of claim 3 , wherein the first vessel section has an aspect ratio of greater than 0.3:1.5. The variable diameter bioreactor vessel of claim 3 , wherein the second vessel section has an aspect ratio of greater than 0.3:1.6. The variable diameter bioreactor vessel of claim 3 , wherein the first vessel section is configured such that an inoculum could be added directly to said bioreactor.7. The variable diameter bioreactor vessel of claim 3 , wherein the second vessel section is configured to be a growth stage bioreactor.8. The variable diameter bioreactor vessel of claim 3 , wherein at least one of the vessels of the variable diameter bioreactor is configured to be a production stage bioreactor.930-. (canceled)31. A method of producing a fermentation product using a reduced amount of reactors in a seed stage train and production reactor claim 3 , comprising the steps of:inoculating a variable ...

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. An automated stand-alone multi-module cell editing instrument for performing recursive nuclease editing comprising:a receptacle configured to receive cells;one or more receptacles configured to receive nucleic acids;a growth module for growing cells for transformation;a filtration module to concentrate the cells and render the cells electrocompetent;a transformation module configured to introduce the nucleic acids into the cells;a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells;a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; andan automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the filtration module, from the filtration module to the transformation module, from the transformation module to the nuclease-directed editing module, and from the nuclease-directed editing module to the growth module, without user intervention.2. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the nuclease-directed editing module is also a selection module.3. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the nuclease-directed editing module is also a recovery module.4. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the automated liquid handling system comprises a sipper or pipettor.5. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the recursive nuclease editing system employs an RNA-guided nuclease.6. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the recursive nuclease editing system employs a ...

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. An automated stand-alone multi-module cell editing instrument comprising:a housing configured to house all or some of the modules;a receptacle configured to receive cells;a receptacle configured to receive nucleic acids;a growth module for growing cells for transformation, wherein the growth module comprises a growth vial comprising one or more paddles;a transformation module configured to introduce the nucleic acids into the cells;a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells;a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; andan automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the transformation module and from the transformation module to the nuclease-directed editing module without user intervention.2. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the nucleic acids in the receptacle comprise an editing cassette.3. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the automated liquid handling system comprises a sipper or pipettor.4. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the growth vial is fabricated from polycarbonate or polypropylene.5. The automated stand-alone multi-module cell editing instrument of claim 4 , wherein the growth vial is fabricated by injection molding.6. The automated stand-alone multi-module cell editing instrument of claim 1 , wherein the editing module is also a recovery module.7. The automated stand-alone multi-module cell editing instrument of claim 1 , further comprising a recovery module separate from the ...

SYSTEM AND METHOD FOR INCUBATION AND READING OF BIOLOGICAL CULTURES

The present invention describes an integrated incubator and image capture module that regulates the incubator atmosphere and obtains high-resolution digital images of sample specimens. The incubator has a cabinet type enclosure that enables the provision of a controlled environment to the contents of the incubator by having at least three ports on one face of the cabinet for the passage of sample containers. Additionally, an image capture module is located immediately adjacent to the incubator. In this regard, using at least three separate access/egress points for the sample containers streamlines operation of the system and enhances preservation of the incubator environment. Furthermore, locating the image capture module directly adjacent to the incubator reduces the amount of time a sample container is exposed to the external environment, thereby reducing the extent to which samples are exposed to potential contaminants and reducing the exchange of the lab and ambient atmospheres. 1. An integrated system for incubating and monitoring biological samples disposed in culture media , comprising:an incubator comprising a cabinet with multiple ports for automated receipt and removal of sample containers from the cabinet, wherein the cabinet includes a first port, a second port, and a third port;a first conveyor adapted to cooperate with the first port to receive sample containers containing biological samples inoculated with a sample for incubation,a second conveyor adapted to cooperate with the second port to remove sample containers from the cabinet, andwherein the third port cooperates with an image capture conveyor in an image capture module, wherein the image capture conveyor conveys sample containers from the cabinet to the image capture module for imaging and returns the sample containers to the cabinet after imaging.2. The system of claim 1 , wherein the third port further comprises:an ingress port adapted to receive sample containers in to the image capture ...

METHOD FOR EXTRACTING OIL FROM A WATER AND SOLIDS COMPOSITION, METHOD FOR THE PRODUCTION OF ETHANOL, AND ETHANOL PRODUCTION FACILITY

The present disclosure includes a method for processing a beer stream for the recovery of oil. The method include a step of extracting oil from a beer stream into an organic phase comprising an organic solvent to provide in the organic phase at least a portion of the oil. In general, a beer stream refers to a composition containing alcohol, water, oil, and particulates, and can be a result of a fermentation process. When the beer stream is a beer stream from a fermentation process, it can be referred to as a fermentation broth even if it is no longer being subjected to feinientation. The beer stream can contain other components commonly found in a stream coming off a fermentation process such as, for example, glycerol and acetic acid. A method for producing ethanol, and an ethanol production facility are provided. 124-. (canceled)25. An ethanol production facility comprising:(a) a source of fermentation broth containing oil, ethanol, water, and particulates;(b) a liquid-liquid extractor for the recovery of at least a portion of the oil from the fermentation broth, the extractor comprising an organic phase inlet, a fermentation broth inlet constructed to receive fermentation broth from the source of fermentation broth, a miscella outlet, and a deoiled fermentation broth outlet; and(c) a distillation system constructed to receive deoiled fermentation broth from the liquid-liquid extractor and provide a volatile stream comprising concentrated ethanol.26. An ethanol production facility according to claim 25 , wherein the source of fermentation broth comprises a beer well constructed to provide a continuous stream of the fermentation broth to the liquid-liquid extractor.27. An ethanol production facility according to claim 26 , further comprising:(a) a fermentor that feeds the fermentation broth to the beer well.28. An ethanol production facility according to claim 26 , further comprising:(a) a plurality fermentors that feed the fermentation broth to the beer well.29. An ...

FACILITY FOR COUPLING A BIOREACTOR WITH A DEVICE FOR PHYSICOCHEMICALLY ANALYSING OR COLLECTING SAMPLES

The invention concerns a facility for coupling a bioreactor for culturing bio-organisms, in dynamic mode, with at least one device for physicochemically analyzing a fluid from this bioreactor or collecting samples of this fluid, the device being equipped with a pump and an injector, the bioreactor being equipped with means for supplying culture medium and xenobiotic(s). The facility is remarkable in that the bioreactor is connected to a closed perfusion loop provided with a pump, fitted with a sampling loop and in that the facility comprises: 2. Installation according to claim 1 , wherein it comprises a third valve fitted with at least one input and two outputs claim 1 , this third valve being positioned on the injection loop such that its input is connected to the injector claim 1 , its first output is connected to at least one of the units for analysis or collecting and its second output is connected to a drain tank claim 1 , said third valve occupying either a first position in which the input is connected to its first output claim 1 , or a second position in which the input is connected to its second output.3. The facility according to or :for the first valve, its first and second connecting orifices are connected to the closed perfusion loop of the bioreactor, its third and sixth connecting orifices are connected to the sample loop, its fourth connecting orifice to the third connecting orifice of the second valve and its fifth connecting orifice to the sixth connecting orifice of the second valve, said first valve occupying either a first position, in which its first connecting orifice is connected to its second, its third to its fourth and its fifth to its sixth, or a second position, in which its first connecting orifice is connected to its sixth, its second to its third and its fourth to its fifth,for the second valve, its first connecting orifice is connected to a drain tank, its second connecting orifice to the injection device of a cleaning product, its ...

Combined system for producing steel and method for operating the combined system

The invention relates to a plant complex for steel production comprising a blast furnace for producing pig iron, a converter steel mill for producing crude steel and a gas-conducting system for gases that occur in the production of pig iron and/or in the production of crude steel. According to the invention, the plant complex additionally has a chemical or biotechnological plant connected to the gas-conducting system and a plant for producing hydrogen. The plant for producing hydrogen is connected to the gas-conducting system by a hydrogen-carrying line. Also the subject of the invention is a method for operating the plant complex.

Method and system for providing buffer solutions

A method for delivering a processing solution includes providing a concentrated processing solution in a bioprocess container, the concentrated processing solution being produced at a first site. The method also includes combining the concentrated processing solution with a biopolymer containing solution produced in a bioreactor at a second site different from the first site. In some embodiments, the processing solution is a buffer for processing products of cells cultured in a bioreactor. A system and a pharmaceutical production facility for carrying out the method is also provided.

Automated cell processing methods, modules, instruments, and systems

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

Purification of Biological Molecules

The present invention relates to improved processes and systems for purification of biological molecules, where the processes can be performed in a continuous manner. 2. The process of claim 1 , wherein the process is a continuous process.3. The process of claim 1 , comprising a virus inactivation step between steps (c) and (d).4. The process of claim 3 , wherein the virus inactivation step comprises use of a virus inactivating agent selected from acid claim 3 , detergent claim 3 , solvent and temperature change.5. The process of claim 3 , wherein virus inactivation step comprises use of one or more in-line static mixers.6. The process of claim 3 , wherein virus inactivation comprises use of one or more surge tanks.7. The process of claim 1 , wherein the target molecule is an antibody.8. The process of claim 7 , wherein the antibody is selected from a monoclonal antibody or a polyclonal antibody.9. The process of claim 1 , wherein the precipitant in step (b) is a stimulus responsive polymer.10. The process of claim 9 , wherein the stimulus responsive polymer is a modified polyallylamine polymer.11. The process of claim 1 , wherein the precipitant in step (b) is selected from the group consisting of an acid claim 1 , caprylic acid claim 1 , a flocculant and a salt.12. The process of claim 1 , wherein removing impurities in step (b) comprises use of one or more depth filters.13. The process of claim 1 , wherein removing impurities in step (b) comprises use of centrifugation.14. The process of claim 1 , wherein the bind and elute chromatography step in (c) employs continuous multi-column chromatography.15. The process of claim 1 , wherein the bind and elute chromatography step in (c) is selected from the group consisting of affinity chromatography claim 1 , cation exchange chromatography and mixed-mode chromatography.16. The process of claim 1 , wherein the bind and elute chromatography step in (c) employs Protein A affinity chromatography.17. The process of claim 16 , ...

Method and Apparatus for Reducing CO2 in a Stream by Conversion to a Syngas for Production of Energy

A system and method for producing Syngas from the COin a gaseous stream, such as an exhaust stream, from a power plant or industrial plant, like a cement kiln, is disclosed. A preferred embodiment includes providing the gaseous stream to pyrolysis reactor along with a carbon source such as coke. The COand carbon are heated to about 1330° C. and at about one atmosphere with reactants such as steam such that a reaction takes place that produces Syngas, carbon dioxide (CO) and hydrogen (H). The Syngas is then cleaned and provided to a Fischer-Tropsch synthesis reactor to produce Ethanol or Bio-catalytic synthesis reactor. 1. An apparatus for producing syngas that reduces an amount of carbon dioxide in a gaseous stream , the apparatus comprising:{'sub': 2', '2, 'a reaction chamber, said reaction chamber selected to be one of a pyrolysis reactor, a conventional gasifier or a plasma arc gasifier and wherein said reaction chamber includes a input line for receiving a gaseous stream provided by a source separate from said reaction chamber, and said reaction chamber capable of operating at a preferred temperature of about 1330° C. and up to about 2000° C. and a pressure of about one bar or greater and capable of supporting a reaction in said reaction chamber to form syngas comprising carbon monoxide (CO) and hydrogen (H), and carbon dioxide (CO);'}a source of carbonaceous material provided as a feed to said reaction chamber;{'sub': '2', 'a source of HO provided as a feed to said reaction chamber;'}{'sub': '2', 'a selected one of a power plant or an industrial plant capable of providing said gaseous stream from said separate source as a feed to said reaction chamber at said input line, said gaseous stream being a gaseous exhaust containing between about 30% to about 45% carbon dioxide (CO);'}{'sub': 2', '2', '2', '2, 'said reaction chamber further capable of supporting a reaction that consists essentially of said carbonaceous material, said HO and said carbon dioxide (CO) in ...

DEVICE FOR SUPPORTING AN IMAGE CAPTURING DEVICE ON A BIOREACTOR, BIOREACTOR WITH DEVICE FOR SUPPORTING AN IMAGE CAPTURING DEVICE, AND METHOD FOR PROPAGATION OR CULTIVATION OF BIOLOGICAL MATERIAL

A supporting device for an image capturing device on a bioreactor, a bioreactor, and a method of use are provided. The supporting device for an image capturing device includes a window and a holder. The window has a transparent element that is transparent to electromagnetic radiation. The holder is configured to hold the image capturing device. The supporting device is held at least partially in a through-opening of a feedthrough of a container for holding fluid media containing biological material. The window seals the through-opening. 1. A bioreactor , comprising:a container having an exterior and an interior configured to hold fluid media containing biological material or a precursor of the biological material;a feedthrough with a through-opening between the interior and the exterior; anda supporting device for an image capturing device, the supporting device having a window and a holder for the image capturing device, the window having a transparent element that is transparent to electromagnetic radiation, the supporting device is held at least partially in the through-opening of the feedthrough with the window sealing the through-opening.2. The bioreactor of claim 1 , wherein the container comprises a material selected from a group consisting of stainless steel claim 1 , plastic claim 1 , and a sterilizable plastic.3. The bioreactor of claim 1 , wherein the container including the supporting device held at least partially in the through-opening is autoclavable.4. A device for supporting an image capturing device on a bioreactor claim 1 , comprising:a window including a transparent element, the transparent element is transparent to electromagnetic radiation; anda holder for the image capturing device.5. The device of claim 4 , wherein the holder comprises a main body on which the transparent element of the window is secured in a hermetically sealed manner.6. The device of claim 5 , wherein the transparent element is secured to the main body by a compression ...

SENSOR RECEPTACLE FOR A BIOREACTOR, AND BIOREACTOR WITH SENSOR RECEPTACLE, AND METHOD FOR PROPAGATION OR CULTIVATION OF BIOLOGICAL MATERIAL

A sensor receptacle for a bioreactor and to a bioreactor having a sensor receptacle are provided, as are methods of use. The sensor receptacle has a window and extends at least partially in a through-opening of a container for holding fluid media containing biological material. The sensor receptacle and the window seal the through-opening. 1. A bioreactor , comprising:a container having an interior configured to hold fluid media containing biological material and an exterior;a feedthrough with a through-opening between the interior and the exterior;a sensor receptacle that extends at least partially in through-opening, the sensor receptacle having a window that seals the through-opening.2. The bioreactor of claim 1 , wherein the container comprises a material selected from the group consisting of stainless steel claim 1 , plastic claim 1 , and a sterilizable plastic.3. The bioreactor of claim 1 , wherein the sensor receptacle extends in the feedthrough in a form-fitting manner with respect to the feedthrough.4. The bioreactor of claim 1 , wherein the bioreactor is autoclavable.5. A sensor receptacle for supporting at least one sensor for a bioreactor claim 1 , comprising:a holder body with a sensor receiving area; anda window arranged on the holder body, the window having a transparent element that is transmissive to electromagnetic radiation.6. The sensor receptacle of claim 5 , wherein the holder body has a cylindrically symmetrical shape and the sensor receiving area is hermetically sealed by the window.7. The sensor receptacle of claim 5 , wherein the transparent element exhibits a transmittance of greater than 80% in a spectral range of wavelengths between 250 and 2000 nm.8. The sensor receptacle of claim 5 , wherein the transparent element comprises a material selected from the group consisting of glass claim 5 , quartz glass claim 5 , and borosilicate glass.9. The sensor receptacle of claim 5 , wherein the transparent element is secured to a main body by a ...

AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. 1. A method of creating a cell library of at least 5 ,000 edited cells using an automated stand-alone multi-module cell editing instrument for nuclease-directed genome editing , comprising the steps of:providing live cells in a receptacle configured to receive cells;providing a library of rationally-designed nucleic acids comprising sequences to facilitate nuclease-directed genome edits in the cells in a receptacle configured to receive nucleic acids;growing the live cells in a growth module;transforming the live cells in a transformation module with the library of rationally-designed nucleic acids;providing conditions in a nuclease-directed editing module to allow nuclease-directed editing of nucleic acids in the cells; andusing an automated liquid handling system to move liquids from the receptacle configured to receive the cells to the growth module, from the growth module to the transformation module, from the receptacle configured to receive the rationally-designed nucleic acids to the transformation module, and from the transformation module to the nuclease-directed editing module without user intervention, wherein the nuclease-directed genome edits created in the automated stand-alone multi-module cell editing instrument result in a cell library comprising at least 5,000 cells with rationally-designed genome edits.2. The method of claim 1 , wherein the rationally-designed genome edits in the cells creates a saturation mutagenesis cell library.3. The method of claim 1 , wherein the rationally-designed genome edits in the cells creates a promoter swap cell library.4. The method of claim 1 , wherein the rationally-designed genome edits in the cells creates a terminator swap cell library.5. The method of claim 1 , wherein the rationally-designed genome edits in the cells creates a SNP swap ...

System and method for the production of biomolecules such as viral vaccines

The current disclosure concerns a system for producing biomolecules comprising a bioreactor including a chamber (1) suitable for receiving a liquid comprising cells and viral particles; and a concentrator (2), wherein said concentrator is equipped with a retentate conduit (300, 303) suitable for collecting said retentate and facilitating recirculating of the retentate to an input of said bioreactor or to an input of an intermediate vessel (4) positioned between said concentrator and said bioreactor. In a second and third aspect the disclosure concerns a method for producing biomolecules and the use of the disclosed system for the production of biomolecules.

SYSTEM AND METHOD FOR PRODUCING A SUGAR STREAM

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biochemical production. In particular, after saccharification and prior to a sugar conversion process, a sugar/carbohydrate stream is removed from a saccharified stream. The sugar/carbohydrate stream includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose can be produced, with the such sugar stream being available for biochemical production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein and/or fiber. Sugar stream production occurs on the front end of the system and method. 1. A method for producing a sugar stream comprising:mixing ground grain particles with a liquid to provide a slurry;separating the slurry into an initial solids portion and an initial liquid portion,subjecting the initial solids portion to liquefaction to provide a first liquefied starch solution including starch and the initial solids and subjecting at least a portion of the initial liquid portion to a separate liquefaction step to provide a second liquefied starch solution including starch;thereafter, subjecting the second liquefied starch solution to saccharification to convert the starch to simple sugars and produce a saccharified stream including the simple sugars; andafter saccharification of the second liquefied starch solution but prior to further processing of the simple sugars, separating the saccharified stream into a solids portion and a liquid portion including the simple sugars,wherein, prior to separating the saccharified stream into the solids portion and the liquid portion, the separated initial solids do not recombine with the saccharified stream of the second liquefied starch solution and wherein the separated liquid portion from the saccharified ...

APPARATUS FOR PRODUCING ORGANIC SUBSTANCE FROM WASTE AND METHOD FOR PRODUCING ORGANIC SUBSTANCE FROM WASTE

The present invention provides an apparatus and a method which are suitable for producing an organic substance using a synthesis gas from a waste gasification furnace. The apparatus for producing an organic substance from waste comprises a synthesis gas generation furnace for generating a synthesis gas by partial oxidation of the waste; and an organic substance production unit for producing an organic substance from the synthesis gas. The organic substance production unit further comprises: a synthesis unit for synthesizing an organic substance by subjecting the synthesis gas to catalytic reaction in the presence of a metal catalyst, and a fermenter for producing an organic substance by subjecting the synthesis gas to microbial fermentation. 16-. (canceled)7. A method for producing an organic substance from waste , comprising:a synthesis gas-generating step for generating a synthesis gas by partial oxidation of waste; andan organic substance-producing step for producing an organic substance from the synthesis gas,the organic substance-producing step further comprising:a synthesizing step for synthesizing an organic substance by subjecting the synthesis gas to catalytic reaction in the presence of a metal catalyst in a synthesis unit; anda fermentation step for producing an organic substance by subjecting the synthesis gas to microbial fermentation in a fermenter.8. The method according to claim 7 , wherein the fermentation step is performed after the synthesizing step.9. The method according to claim 7 , wherein the organic substance is ethanol.10. The method according to claim 7 , wherein claim 7 , the organic substance synthesized in the synthesizing step and the organic substance produced in the fermentation step are different from each other.1112-. (canceled) The present invention relates to an apparatus and a method for producing an organic substance from waste.In recent years, the practical application has been considered with respect to a method for producing ...

Automated cell processing methods, modules, instruments, and systems

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

CELL CULTURE APPARATUS, IMAGING UNIT, AND CULTURE MONITORING METHOD

A cell culture apparatus includes a flow passage in which cell suspension containing at least one of cells or cell masses as granular bodies is to flow, and an imaging unit that is provided in a middle of the flow passage and continuously images the plurality of granular bodies contained in the cell suspension to acquire a plurality of images while the cell suspension flows in the flow passage.

DEVICES AND METHODS FOR BIO-PROCESSING CELLULAR SAMPLES

The present disclosure relates to completely closed systems suitable for bio-processing of cellular samples, for example peripheral blood samples used for immunotherapy applications, and related methods of use. The systems are not open to the air, thus allowing for sterile sample processing and transfer of the sample throughout the entirety of bio-processing. Each component of the disclosed systems contains a unique identifier, allowing for traceability of the sample as it proceeds through the various steps involved in bio-processing. The identifier ultimately traces the sample back to the patient from which the sample was derived. Certain embodiments provide a unique freezing bag for long-term storage of cellular samples. The freezing bag has a unique identifier that allows for easy traceability and retrieval of a bio-archived sample and at least two ports, one for sample testing, another for sterile docking to a device that allows for delivery of its contents to a patient. 1. A system for cellular bioprocessing and manufacturing , comprising: one or more processing bagsets , a culture bagset and a washing bagset , wherein:each bagset comprises the same 2D barcode that is unique to the system; andeach bagset is configured to be in fluid connection with the other bagsets via one or more luer connections or via one or more sterile docks using a sterile connection device.2. The system of claim 1 , wherein the one or more processing bagsets comprise flexible components.3. The system of or claim 1 , wherein the flexible components comprise a processing bag claim 1 , a red blood cell bag claim 1 , and a cell concentrate bag;wherein the system is closed to the outside environment and the components are in fluid connection with each other via a plurality of tubes.4. The system of claim 3 , wherein:the processing bag is in fluid connection with the red blood cell bag via a first tube;the processing bag is in fluid connection with the cell concentrate bag via a second tube; ...