09-05-2013 дата публикации
Номер: US20130115663A1
Принадлежит:
CODEXIS, INC.
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations. 1. An enone reductase polypeptide comprising an amino acid sequence that is at least 80% identical to a reference sequence selected from the group consisting of SEQ ID NOs: 8 , 190 , 192 , 194 , 196 , 198 , 200 , 202 , 204 , 206 , 208 , 210 , 212 , 214 , 216 and 217; and wherein the amino acid sequence comprises one or more residue differences as compared to the reference sequence selected from the group consisting of: residue corresponding to X5 is E; residue corresponding to X10 is P; residue corresponding to X28 is P; residue corresponding to X38 is N or S; residue corresponding to X40 is L , Y , E or S; residue corresponding to X44 is Y; residue corresponding to X75 is L or S; residue corresponding to X83 is L , R , V , I , K , E , or M; residue corresponding to X117 is C , L , A , M , V , I , N , Q , E , F , or S; residue corresponding to X119 is P or V; residue corresponding to X122 is T; residue corresponding to X124 is G or P; residue corresponding to X147 is G; residue corresponding to X148 is R; residue corresponding to X153 is E; residue corresponding to X154 is R; residue corresponding to X179 is R; residue corresponding to X209 is D; residue corresponding to X240 is R; residue corresponding to X248 is C; residue corresponding to X251 is A; C , R , E , D , W , Y , R , S , V , G , L , or I; residue corresponding to X252 is H; residue corresponding to X255 is P; residue corresponding to X259 is G; residue corresponding to X294 is A; residue corresponding to X295 is T , N , or G; residue corresponding to X296 is G , F , A , S , R , E , Q , K , or I; residue corresponding to X297 is F; K; Y , W , G , ...
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